##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936310_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3049187 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78541296417701 33.0 31.0 34.0 30.0 34.0 2 31.880636379467706 34.0 31.0 34.0 30.0 34.0 3 31.95294188254115 34.0 31.0 34.0 30.0 34.0 4 35.440037951099754 37.0 35.0 37.0 33.0 37.0 5 35.42305112805479 37.0 35.0 37.0 33.0 37.0 6 35.50699350351422 37.0 35.0 37.0 33.0 37.0 7 35.45061388494704 37.0 35.0 37.0 33.0 37.0 8 35.45018885361901 37.0 35.0 37.0 33.0 37.0 9 37.13132320188955 39.0 37.0 39.0 34.0 39.0 10-11 37.055764372601615 39.0 37.0 39.0 33.5 39.0 12-13 36.96496541537138 39.0 37.0 39.0 33.0 39.0 14-15 38.24287736370383 40.0 38.0 41.0 33.0 41.0 16-17 38.19194575472085 40.0 38.0 41.0 33.0 41.0 18-19 38.12798329521935 40.0 38.0 41.0 33.0 41.0 20-21 38.04141792549949 40.0 38.0 41.0 33.0 41.0 22-23 37.89194644342902 40.0 38.0 41.0 33.0 41.0 24-25 38.03587759622483 40.0 38.0 41.0 33.0 41.0 26-27 37.99027708041521 40.0 38.0 41.0 33.0 41.0 28-29 37.8657533959052 40.0 38.0 41.0 33.0 41.0 30-31 37.73003066063183 40.0 38.0 41.0 32.5 41.0 32-33 37.57520348866763 40.0 38.0 41.0 32.0 41.0 34-35 37.32964606631211 40.0 37.0 41.0 31.5 41.0 36-37 37.14331164339872 40.0 37.0 41.0 31.0 41.0 38-39 36.990246088547536 40.0 37.0 41.0 31.0 41.0 40-41 36.816644239923626 40.0 36.5 41.0 30.0 41.0 42-43 36.63698028359691 40.0 36.0 41.0 30.0 41.0 44-45 36.468904826106105 40.0 36.0 41.0 30.0 41.0 46-47 36.231146695824165 39.5 35.5 41.0 29.5 41.0 48-49 36.09029013307482 39.0 35.0 41.0 29.0 41.0 50-51 35.78346752757374 39.0 35.0 40.5 28.5 41.0 52-53 35.79211868606288 39.0 35.0 40.5 29.0 41.0 54-55 35.8614235532291 39.0 35.0 41.0 29.0 41.0 56-57 35.68576476286958 39.0 35.0 41.0 28.0 41.0 58-59 35.44095819639792 38.5 35.0 41.0 28.0 41.0 60-61 35.14103693869874 38.0 35.0 40.0 28.0 41.0 62-63 34.829305319745885 37.5 34.5 40.0 27.5 41.0 64-65 34.493927397696496 37.0 34.0 40.0 26.5 41.0 66-67 34.13283917975512 36.5 34.0 39.5 26.0 41.0 68-69 33.75554647845475 36.0 34.0 39.0 26.0 41.0 70-71 33.357296059572604 35.0 33.5 39.0 26.0 40.5 72-73 32.96901649521659 35.0 33.0 37.5 25.0 40.0 74-75 32.56618911860768 35.0 33.0 37.0 24.5 39.0 76-77 32.186666970572816 35.0 33.0 36.5 23.5 39.0 78-79 31.814697819451546 35.0 32.0 36.0 22.5 38.5 80-81 31.449588201707535 35.0 32.0 36.0 20.5 37.0 82-83 31.123757906615765 35.0 32.0 35.0 20.0 37.0 84-85 30.828675807682508 35.0 31.5 35.0 18.5 36.5 86-87 30.530324968589987 34.5 31.0 35.0 17.5 36.0 88-89 30.223697497070532 34.0 31.0 35.0 12.5 36.0 90-91 29.90086078682613 34.0 31.0 35.0 7.0 35.5 92-93 29.61614030231665 34.0 30.5 35.0 2.0 35.0 94-95 29.264128274192434 34.0 30.0 35.0 2.0 35.0 96-97 28.863774343784097 34.0 30.0 35.0 2.0 35.0 98-99 28.403807473926655 34.0 29.5 35.0 2.0 35.0 100-101 25.227834993393323 30.5 23.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27168476531158703 1101 2 0.19194979093218123 1101 3 0.2976070160432229 1101 4 0.08078223956612618 1101 5 0.2682198474441293 1101 6 0.17938853866500182 1101 7 0.2778226388282974 1101 8 0.1551719171124546 1101 9 0.14431097823996453 1101 10-11 0.41315804240147713 1101 12-13 0.26595719641922244 1101 14-15 0.41846807034230693 1101 16-17 0.43333328667932136 1101 18-19 0.4627738986324985 1101 20-21 0.4759750564530947 1101 22-23 0.44959238105318633 1101 24-25 0.3721719152120002 1101 26-27 0.370459664829653 1101 28-29 0.3162494186555662 1101 30-31 0.34651320697847154 1101 32-33 0.4188074332985252 1101 34-35 0.2641129592877931 1101 36-37 0.20847681744147195 1101 38-39 0.24290127677517148 1101 40-41 0.07453701348991615 1101 42-43 0.0782306646384825 1101 44-45 0.19488804007749394 1101 46-47 -0.05190088915428959 1101 48-49 -0.010875253971242671 1101 50-51 0.09504660754865313 1101 52-53 0.13127241178737847 1101 54-55 0.12740506613808122 1101 56-57 0.09141181185819391 1101 58-59 0.12159164101527153 1101 60-61 0.08611587711491353 1101 62-63 0.04908051019317128 1101 64-65 0.04515664741122549 1101 66-67 0.21260646629568924 1101 68-69 0.19208956596050797 1101 70-71 0.2626723226468428 1101 72-73 0.233831474137574 1101 74-75 0.10589233374825824 1101 76-77 0.08307737027453754 1101 78-79 0.044846393135703266 1101 80-81 0.20028191494933267 1101 82-83 0.2712341646961356 1101 84-85 0.24632930356255756 1101 86-87 0.23951160724392295 1101 88-89 0.1507083999012977 1101 90-91 0.3073617680737897 1101 92-93 0.2693545265171444 1101 94-95 0.5122487592089016 1101 96-97 0.297840718017337 1101 98-99 0.25268873784678547 1101 100-101 0.5551230485222725 1102 1 0.310124002586182 1102 2 0.32707472439240703 1102 3 0.2862765579123234 1102 4 0.2150852327193178 1102 5 0.34482541728119287 1102 6 0.2946675364404143 1102 7 0.18038260527612238 1102 8 0.23895586339378383 1102 9 0.27869726525477034 1102 10-11 0.39100972067013373 1102 12-13 0.35711690213380365 1102 14-15 0.45602113718417314 1102 16-17 0.3835704835475866 1102 18-19 0.4303393715671291 1102 20-21 0.3356696260745977 1102 22-23 0.4436896618312218 1102 24-25 0.4668491734948219 1102 26-27 0.46246360075789994 1102 28-29 0.2707590920351137 1102 30-31 0.4601663613668663 1102 32-33 0.19616286785638692 1102 34-35 0.19949503321695516 1102 36-37 0.25972028627887056 1102 38-39 0.2666956787824333 1102 40-41 0.2567270848029537 1102 42-43 0.1882158829109315 1102 44-45 0.04655443056439168 1102 46-47 0.17375926602452552 1102 48-49 0.21174166905378655 1102 50-51 0.027152945067690837 1102 52-53 0.1297241431412104 1102 54-55 0.17062035584087454 1102 56-57 0.26122255295359764 1102 58-59 0.24220027278160927 1102 60-61 0.2098781640593259 1102 62-63 0.33670096000935246 1102 64-65 0.2304895955593338 1102 66-67 0.289374444498506 1102 68-69 0.34409125823368214 1102 70-71 0.43772735085384085 1102 72-73 0.3077432647649587 1102 74-75 0.31307019693809224 1102 76-77 0.2685895175404269 1102 78-79 0.1876463866833582 1102 80-81 0.2131475903994975 1102 82-83 0.1659337862076562 1102 84-85 0.1883413049175502 1102 86-87 0.11877297546291743 1102 88-89 0.0711169357623831 1102 90-91 0.11009800075695253 1102 92-93 0.19327012989171877 1102 94-95 0.10280561093556173 1102 96-97 0.15573288472824132 1102 98-99 0.1098042039624616 1102 100-101 0.25580245074217345 1103 1 0.34480682426248066 1103 2 0.25100822218019303 1103 3 0.37391984262598044 1103 4 0.2687481161791965 1103 5 0.3362226766251126 1103 6 0.2190468717577616 1103 7 0.29216052387116775 1103 8 0.37506134810065106 1103 9 0.35798703701764367 1103 10-11 0.38369739111247014 1103 12-13 0.4033377501303548 1103 14-15 0.492987037402564 1103 16-17 0.5515003093993727 1103 18-19 0.44869042335896125 1103 20-21 0.5063154964196102 1103 22-23 0.5366961513698811 1103 24-25 0.5252912315894847 1103 26-27 0.6127548379004679 1103 28-29 0.4658387543754188 1103 30-31 0.4916386436250235 1103 32-33 0.5703847738684047 1103 34-35 0.3797993965405979 1103 36-37 0.39713230691865675 1103 38-39 0.4132414280497443 1103 40-41 0.4407694393682604 1103 42-43 0.34392973216353084 1103 44-45 0.45162286209890823 1103 46-47 0.48729012386571924 1103 48-49 0.5489918755648304 1103 50-51 0.5300837044625339 1103 52-53 0.47155323686893524 1103 54-55 0.5864847884164348 1103 56-57 0.5121121063239684 1103 58-59 0.4738531814754481 1103 60-61 0.4702194164780522 1103 62-63 0.5291640143358975 1103 64-65 0.4735837815663686 1103 66-67 0.44652524281926276 1103 68-69 0.4588358634864207 1103 70-71 0.5253274945628945 1103 72-73 0.5228911323263219 1103 74-75 0.5702850702591249 1103 76-77 0.5090960919466383 1103 78-79 0.4281863423259189 1103 80-81 0.4393080127004261 1103 82-83 0.5088409807127654 1103 84-85 0.5110760485609696 1103 86-87 0.5232921725027389 1103 88-89 0.4588264171650742 1103 90-91 0.5609574261470591 1103 92-93 0.6570093375682688 1103 94-95 0.5898040437148069 1103 96-97 0.6299750175063501 1103 98-99 0.6793411383202397 1103 100-101 0.9820504280655662 1104 1 0.2788134019159436 1104 2 0.23326003048857658 1104 3 0.2972147881035667 1104 4 0.31489658321444125 1104 5 0.39855195264618004 1104 6 0.25739885270223084 1104 7 0.42063127407330114 1104 8 0.274493178698755 1104 9 0.26821026203457876 1104 10-11 0.3544664411769318 1104 12-13 0.37757946250552266 1104 14-15 0.48344261092587004 1104 16-17 0.5473950123123359 1104 18-19 0.355660059074971 1104 20-21 0.43215491207923407 1104 22-23 0.5066140399649655 1104 24-25 0.5193549539731777 1104 26-27 0.4293931871498273 1104 28-29 0.436915653918696 1104 30-31 0.4352456452202702 1104 32-33 0.5293391782739079 1104 34-35 0.5833867853651427 1104 36-37 0.6130183780272418 1104 38-39 0.4909235700483876 1104 40-41 0.5936070084773277 1104 42-43 0.5720131438022662 1104 44-45 0.6074015273378706 1104 46-47 0.615409319250432 1104 48-49 0.6093229981364559 1104 50-51 0.6829895631631686 1104 52-53 0.5675386283595074 1104 54-55 0.6167575643610164 1104 56-57 0.6570436528373733 1104 58-59 0.6636427949017261 1104 60-61 0.5920434041531522 1104 62-63 0.539136753130947 1104 64-65 0.5044236342793837 1104 66-67 0.6333090215828392 1104 68-69 0.5236776501606641 1104 70-71 0.5018459819211287 1104 72-73 0.5584844869295864 1104 74-75 0.47627324796503245 1104 76-77 0.6024668115550398 1104 78-79 0.5752294030107841 1104 80-81 0.6238822134390425 1104 82-83 0.7166918253184384 1104 84-85 0.48579770018571367 1104 86-87 0.5328467058389812 1104 88-89 0.4763910464345358 1104 90-91 0.48417861886575153 1104 92-93 0.38563906593959274 1104 94-95 0.1868853465709499 1104 96-97 0.3743419456766297 1104 98-99 0.48466253645402446 1104 100-101 0.5662708887198313 1105 1 0.1843093029309948 1105 2 0.17008289707173319 1105 3 0.1425984374957494 1105 4 0.029357564468675434 1105 5 0.0784958477733042 1105 6 0.140212272853816 1105 7 0.22376928378984218 1105 8 0.2374344130583097 1105 9 0.05923557623076192 1105 10-11 0.22913620081795472 1105 12-13 0.2430459396084288 1105 14-15 0.27197538579289215 1105 16-17 0.3013715066843119 1105 18-19 0.3196744600934167 1105 20-21 0.2959597181070208 1105 22-23 0.38281172562587784 1105 24-25 0.34107946952031654 1105 26-27 0.33589603662618117 1105 28-29 0.3258976394281703 1105 30-31 0.31371666965504375 1105 32-33 0.38668535444781327 1105 34-35 0.257910472373446 1105 36-37 0.3385165325548627 1105 38-39 0.3711023958243871 1105 40-41 0.35125121888683 1105 42-43 0.5525116883105738 1105 44-45 0.4550895961796684 1105 46-47 0.32892355040480936 1105 48-49 0.2867842997506571 1105 50-51 0.2141335126258639 1105 52-53 0.24681144262243748 1105 54-55 0.44328198317201384 1105 56-57 0.4944661343604082 1105 58-59 0.47041619675300694 1105 60-61 0.3751540079113127 1105 62-63 0.20673906809819442 1105 64-65 0.18428187760743953 1105 66-67 0.2101237011888344 1105 68-69 0.1884863685584861 1105 70-71 0.21911875011922177 1105 72-73 0.2815315866510488 1105 74-75 0.33082648189952835 1105 76-77 0.3467613809823362 1105 78-79 0.2959459755149929 1105 80-81 0.4481398367763276 1105 82-83 0.38255715582576144 1105 84-85 0.42924336802371954 1105 86-87 0.36570608613087785 1105 88-89 0.334173137264969 1105 90-91 0.5433191249821654 1105 92-93 0.32750877993455774 1105 94-95 0.3476280582506348 1105 96-97 0.46801524184052923 1105 98-99 0.6112094380443587 1105 100-101 0.5621194919346344 1106 1 0.22939348593114417 1106 2 0.2988249837913095 1106 3 0.30094865329495235 1106 4 0.22947305533203632 1106 5 0.33341789966097934 1106 6 0.2654898866092239 1106 7 0.23416459970705716 1106 8 0.2380475314393493 1106 9 0.26710711578897417 1106 10-11 0.32072790444604493 1106 12-13 0.32808380232428647 1106 14-15 0.35345967861812966 1106 16-17 0.3310355347582501 1106 18-19 0.26985606226735825 1106 20-21 0.33681543306464334 1106 22-23 0.30251819520095324 1106 24-25 0.3525270018010431 1106 26-27 0.28569045606010945 1106 28-29 0.3710989320860918 1106 30-31 0.4129628679227082 1106 32-33 0.4710536306809132 1106 34-35 0.3193997455208475 1106 36-37 0.4107422066594282 1106 38-39 0.37753463745372784 1106 40-41 0.25621445518060426 1106 42-43 0.2979290141406281 1106 44-45 0.4630295930356709 1106 46-47 0.3812874391991983 1106 48-49 0.46943091225262634 1106 50-51 0.5158671149657295 1106 52-53 0.4499253707168478 1106 54-55 0.32823445448298827 1106 56-57 0.5156364240702231 1106 58-59 0.3433619561376844 1106 60-61 0.5099190215654588 1106 62-63 0.5078556947734683 1106 64-65 0.3762150583272401 1106 66-67 0.49869125657784963 1106 68-69 0.596857420012249 1106 70-71 0.5931775571259195 1106 72-73 0.6051197355077989 1106 74-75 0.6485004665708161 1106 76-77 0.6846699163161318 1106 78-79 0.6112975593219439 1106 80-81 0.5921694131618658 1106 82-83 0.7370200139160481 1106 84-85 0.7524308020309718 1106 86-87 0.5705775443699643 1106 88-89 0.4625305706107916 1106 90-91 0.46431259934368896 1106 92-93 0.4354685184942042 1106 94-95 0.6225972979952274 1106 96-97 0.5900661430600458 1106 98-99 0.6512316635856727 1106 100-101 0.5564040094687712 1107 1 -0.048825305243489936 1107 2 0.013135576796106108 1107 3 0.09701421725228698 1107 4 0.054123094525948545 1107 5 -0.037712123354772586 1107 6 0.07002462060245307 1107 7 0.016645456005704773 1107 8 0.09910427461372251 1107 9 0.16288891288726148 1107 10-11 0.15197224205431326 1107 12-13 0.09816066848504335 1107 14-15 0.25629002359477937 1107 16-17 -0.022107666259586267 1107 18-19 0.15812963221919318 1107 20-21 0.28922207453613424 1107 22-23 0.24580581179109373 1107 24-25 0.24852195181836834 1107 26-27 0.2665253665749532 1107 28-29 0.3039983401977153 1107 30-31 0.2678575118447597 1107 32-33 0.27259389393636013 1107 34-35 0.12770203516328849 1107 36-37 0.174958195260551 1107 38-39 0.16721898969863247 1107 40-41 0.3541375948469536 1107 42-43 0.060734048284160735 1107 44-45 0.21275535482062935 1107 46-47 0.27492227851739415 1107 48-49 0.21451313206778622 1107 50-51 0.28826164664975806 1107 52-53 0.400104997906503 1107 54-55 0.3353843145595903 1107 56-57 0.36595122689483617 1107 58-59 0.5090780539308355 1107 60-61 0.4341884844664534 1107 62-63 0.4887390100052471 1107 64-65 0.3723015601388795 1107 66-67 0.3587654038635364 1107 68-69 0.39022622541694574 1107 70-71 0.3994445310576964 1107 72-73 0.21410682939321646 1107 74-75 0.18153022971083743 1107 76-77 0.2476137945232395 1107 78-79 0.2851651836118201 1107 80-81 0.30354201863481123 1107 82-83 0.4757135394838663 1107 84-85 0.3315519869148744 1107 86-87 0.151471427854581 1107 88-89 0.39087880963923993 1107 90-91 0.5085666283576451 1107 92-93 0.28012983578851447 1107 94-95 0.3822144411845869 1107 96-97 0.4977572221700406 1107 98-99 0.4315389428207652 1107 100-101 0.5554037336082374 1108 1 -0.08822468765516334 1108 2 -0.044439287321203835 1108 3 -0.043683540123009124 1108 4 -0.1279886888345203 1108 5 -0.07849505008702806 1108 6 -0.1301772857011514 1108 7 -0.10136720007235311 1108 8 -0.05689732396901803 1108 9 -0.15228013471224244 1108 10-11 -0.1134248182454698 1108 12-13 -0.09484565829127689 1108 14-15 -0.20198949225942187 1108 16-17 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-1.5737428024501803 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23707.0 3 5278.0 4 4398.0 5 3765.0 6 3769.0 7 3865.0 8 4552.0 9 5417.0 10 6234.0 11 7575.0 12 9855.0 13 13787.0 14 14248.0 15 11341.0 16 12906.0 17 15499.0 18 14337.0 19 12032.0 20 12340.0 21 12632.0 22 13756.0 23 15355.0 24 17728.0 25 20844.0 26 24535.0 27 30030.0 28 36471.0 29 44768.0 30 55303.0 31 68372.0 32 87826.0 33 115692.0 34 164292.0 35 248459.0 36 424244.0 37 697191.0 38 663501.0 39 123206.0 40 77.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.42295725066057 17.419579013059828 12.469489717472896 24.687974018806706 2 16.19408631969848 18.723549270884003 39.32231030339845 25.760054106019066 3 20.169262599413816 22.59228986982727 27.783521249826165 29.454926280932753 4 13.062584986713702 14.540017304000058 35.244043468592714 37.153354240693524 5 14.698505606394901 36.75470960458574 33.3528265171147 15.193958271904659 6 35.42448954826395 33.88474638530804 16.30096932943918 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23.59561655302624 26.031530011142635 26.61636245014598 23.756490985685144 30-31 23.737289665812163 25.69897920442463 26.58817984225992 23.975551287503286 32-33 23.513939824513585 25.608267298787744 26.710772790323222 24.167020086375448 34-35 23.98747102415384 25.78812904036207 26.556795617916546 23.667604317567548 36-37 24.111702181115866 25.50191662535547 26.478294199314867 23.908086994213797 38-39 23.82572686705034 25.702204732166727 26.45177775445722 24.02029064632572 40-41 24.016334811230703 25.62854304431698 26.614774656490614 23.740347487961703 42-43 23.99109297265859 25.515535809759342 26.400059973325053 24.093311244257016 44-45 23.802521191883503 25.856808600639468 26.418926389957022 23.921743817520007 46-47 24.076690980181088 25.923745730330765 26.18804622803243 23.811517061455717 48-49 24.170751005860716 25.919538723886475 26.11364887027005 23.79606139998276 50-51 24.05634526191841 26.126075342058208 26.028416243023965 23.78916315299942 52-53 24.31258069375285 25.80179554209556 26.018945582951282 23.866678181200303 54-55 24.111095364901917 25.852668213457076 26.131930895380723 23.904305526260284 56-57 23.88171356615499 26.22394169253397 25.962802494202307 23.931542247108734 58-59 24.027512214798545 25.801516856191302 26.150511100257905 24.02045982875225 60-61 23.92619214586255 25.995097095825116 26.215872781533072 23.862837976779254 62-63 23.81002987354778 26.042991480494535 26.35357356404392 23.79340508191376 64-65 23.775479448600944 26.001054995383367 26.232867242459264 23.990598313556426 66-67 23.769511145554496 26.15628620344706 26.045921292470474 24.028281358527966 68-69 23.911014740515416 25.838657363806085 26.4206711631921 23.8296567324864 70-71 23.866776551006918 25.775066843377907 26.230877060248996 24.12727954536617 72-73 23.860641762882985 26.09491014564968 26.020497696690835 24.0239503947765 74-75 23.792335435324826 26.316768717488554 25.974467930827917 23.9164279163587 76-77 23.923332313369226 26.104177057859978 26.03116476440889 23.94132586436191 78-79 23.910813986980056 26.12474467954688 26.10811877846918 23.856322555003885 80-81 23.927254307880943 26.250073533010266 25.915702251370682 23.906969907738112 82-83 24.05223607971356 26.308163605292023 25.92682855692318 23.712771758071238 84-85 23.97211189713218 26.37429780870011 25.738607779845406 23.914982514322304 86-87 24.000455463827457 26.194673874552016 25.94878273007721 23.85608793154333 88-89 23.877536759507173 26.25351822986109 25.842158504681027 24.026786505950707 90-91 23.766624685968996 26.44615810678043 25.8998265823536 23.88739062489697 92-93 23.865695753140635 26.23648817595382 26.01143464456327 23.886381426342275 94-95 23.840730914320147 26.146638647361627 26.08787786061302 23.924752577705206 96-97 24.00810568959973 25.89502930989947 26.053258617690318 24.04360638281048 98-99 23.720355547297537 26.093839000893777 25.925131603958974 24.260673847849716 100-101 23.870762941513497 26.096043341933502 25.839916341950303 24.193277374602708 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 111.0 1 92.0 2 149.0 3 248.0 4 219.0 5 207.5 6 411.0 7 772.0 8 1141.5 9 1284.5 10 1182.0 11 1124.0 12 1287.5 13 1552.5 14 1780.0 15 1999.5 16 2252.0 17 2419.5 18 2522.5 19 2609.5 20 2683.5 21 2809.0 22 3001.0 23 3344.5 24 3963.5 25 4890.5 26 6511.0 27 9111.0 28 12106.5 29 15804.0 30 20443.0 31 27223.5 32 35652.0 33 40573.5 34 43550.5 35 48951.5 36 56538.0 37 63044.0 38 67685.5 39 71775.0 40 75751.0 41 80068.0 42 83160.0 43 88318.5 44 95510.5 45 102957.0 46 108573.5 47 112829.5 48 117768.0 49 119161.0 50 118746.0 51 119821.5 52 121328.5 53 123182.0 54 125470.5 55 120465.5 56 110147.0 57 101077.0 58 95829.0 59 89232.0 60 77375.5 61 66357.5 62 55666.5 63 43156.5 64 32349.0 65 24705.0 66 17717.0 67 14061.5 68 11213.0 69 8303.5 70 6647.0 71 4879.0 72 3688.5 73 3033.5 74 2352.5 75 1764.0 76 1202.5 77 696.0 78 458.0 79 291.0 80 171.5 81 108.0 82 69.0 83 50.5 84 42.5 85 42.0 86 38.0 87 35.0 88 37.5 89 30.5 90 32.5 91 31.0 92 26.0 93 22.0 94 22.0 95 28.0 96 33.5 97 28.5 98 22.0 99 19.5 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.047356885622298665 2 0.011740834524087897 3 0.010330622556110858 4 0.0059360085163684614 5 0.008461271807862228 6 0.005640847871908152 7 0.00485375282001399 8 0.008986001842458333 9 0.014200506561257148 10-11 0.0020169310704787865 12-13 0.0083300892992132 14-15 0.008510465248605612 16-17 0.018824689991135343 18-19 0.011330889184559687 20-21 0.0033943474112935676 22-23 0.009691107826446853 24-25 0.021858285503644086 26-27 0.007182242348534216 28-29 0.017578456158969587 30-31 0.014348086883487302 32-33 0.013249433373551705 34-35 0.2648738827759662 36-37 0.46882988809804055 38-39 0.4654519385003281 40-41 0.4856540448322782 42-43 0.47568417417495223 44-45 0.4902126370078319 46-47 0.49654219305014746 48-49 0.5031669097369234 50-51 0.5140878535819549 52-53 0.5030849206690177 54-55 0.4901962391942508 56-57 0.4850473257297765 58-59 0.483833887524773 60-61 0.4807510985715208 62-63 0.47758632055036304 64-65 0.49359058660554433 66-67 0.46750166519796915 68-69 0.4941153166401405 70-71 0.4749134769366392 72-73 0.48368630720254285 74-75 0.4971653099662303 76-77 0.48427662849146347 78-79 0.48422743505072013 80-81 0.4865395267656592 82-83 0.48693307429160626 84-85 0.48690027866444396 86-87 0.48922876819296424 88-89 0.5032816944319912 90-91 0.5262714290727332 92-93 0.5144486054807396 94-95 0.48703146117309304 96-97 0.46028662722227265 98-99 0.45107105598967856 100-101 0.458286093965375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3049187.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.764208343020286 #Duplication Level Percentage of deduplicated Percentage of total 1 66.45185192186177 27.088571365223064 2 14.739507304756575 12.016886932891328 3 6.5454904755781325 8.004652123611658 4 3.5511304699189776 5.79036089316099 5 2.114086682910075 4.308953499867549 6 1.4448983162866909 3.534008159975393 7 0.9763951771995657 2.786138349993832 8 0.7039906959009495 2.2958098719403317 9 0.5227431160263126 1.9178288362418612 >10 2.6931837928733997 19.379475283780433 >50 0.1693770564430001 4.771107209029587 >100 0.08162571367607184 5.852941410368027 >500 0.004188765937576067 1.185246846091327 >1k 0.0015305106309463198 1.0680192178245431 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3732 0.12239328056954198 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.279562716225669E-5 2 0.0 0.0 0.0 0.0 3.279562716225669E-5 3 0.0 0.0 0.0 0.0 3.279562716225669E-5 4 0.0 0.0 0.0 0.0 3.279562716225669E-5 5 0.0 0.0 0.0 0.0 3.279562716225669E-5 6 0.0 0.0 0.0 0.0 3.279562716225669E-5 7 0.0 0.0 0.0 0.0 3.279562716225669E-5 8 0.0 0.0 0.0 0.0 3.279562716225669E-5 9 0.0 0.0 0.0 0.0 3.279562716225669E-5 10-11 0.0 0.0 0.0 0.0 6.559125432451338E-5 12-13 0.0 0.0 0.0 3.279562716225669E-5 2.459672037169252E-4 14-15 0.0 0.0 0.0 3.279562716225669E-5 2.9516064446031024E-4 16-17 0.0 0.0 0.0 1.6397813581128345E-4 3.607518987848236E-4 18-19 0.0 0.0 0.0 2.787628308791819E-4 3.607518987848236E-4 20-21 0.0 0.0 0.0 5.575256617583638E-4 4.099453395282087E-4 22-23 0.0 0.0 0.0 0.0011642447642601127 4.2634315310933704E-4 24-25 0.0 0.0 0.0 0.0017217704260184764 4.2634315310933704E-4 26-27 0.0 0.0 0.0 0.002000533256897658 4.75536593852722E-4 28-29 0.0 0.0 0.0 0.002541661105074894 4.919344074338504E-4 30-31 0.0 0.0 0.0 0.004411011853323526 4.919344074338504E-4 32-33 0.0 0.0 0.0 0.007756165823873708 5.247300345961071E-4 34-35 0.0 0.0 0.0 0.011986801727804822 5.247300345961071E-4 36-37 0.0 0.0 0.0 0.02090721231593864 5.247300345961071E-4 38-39 0.0 0.0 0.0 0.03402546318084132 5.575256617583638E-4 40-41 0.0 0.0 0.0 0.04994774016811694 5.575256617583638E-4 42-43 0.0 0.0 0.0 0.06667351002086785 5.575256617583638E-4 44-45 0.0 0.0 0.0 0.08574416721572012 5.739234753394922E-4 46-47 0.0 0.0 0.0 0.10865191278855643 6.067191025017488E-4 48-49 0.0 0.0 0.0 0.1358230898924861 7.542994247319039E-4 50-51 0.0 0.0 0.0 0.1653883477792605 8.198906790564173E-4 52-53 0.0 0.0 0.0 0.19265791176467695 8.526863062186741E-4 54-55 0.0 0.0 0.0 0.22628982741957118 9.182775605431875E-4 56-57 0.0 0.0 0.0 0.2636768423845438 9.510731877054442E-4 58-59 0.0 0.0 0.0 0.3069014789843981 9.510731877054442E-4 60-61 0.0 0.0 0.0 0.35483228808203626 9.510731877054442E-4 62-63 0.0 0.0 0.0 0.40351739660440633 9.510731877054442E-4 64-65 0.0 0.0 0.0 0.45602319569117933 9.838688148677008E-4 66-67 0.0 0.0 0.0 0.5198762817760931 0.0010166644420299576 68-69 0.0 0.0 0.0 0.591403544616975 0.0010166644420299576 70-71 0.0 0.0 0.0 0.6696375132125383 0.0010494600691922143 72-73 0.0 0.0 0.0 0.7542010378504171 0.001180642577841241 74-75 0.0 0.0 0.0 0.8416505776785747 0.001279029459328011 76-77 0.0 0.0 0.0 0.937905743399798 0.001279029459328011 78-79 0.0 0.0 0.0 1.0513786133812062 0.001279029459328011 80-81 0.0 0.0 0.0 1.1790027964831282 0.0013118250864902676 82-83 0.0 0.0 0.0 1.3145143279175728 0.001459405408720423 84-85 0.0 0.0 0.0 1.4522067685583075 0.0014758032223015512 86-87 0.0 0.0 0.0 1.6016564415367114 0.0015413944766260648 88-89 0.0 0.0 0.0 1.7674219390283377 0.001606985730950578 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3805 0.0 39.07167 1 GTATCAA 6690 0.0 37.7922 1 ATCAACG 8040 0.0 30.557152 3 TCAACGC 8050 0.0 30.518188 4 CAACGCA 8050 0.0 30.400583 5 AACGCAG 8510 0.0 28.978855 6 TATCAAC 8615 0.0 28.571651 2 GTACATG 11885 0.0 26.730701 1 TACATGG 11675 0.0 26.475464 2 ACATGGG 11830 0.0 25.289127 3 ACGCAGA 9960 0.0 24.71252 7 GTGTAGC 1560 0.0 23.976704 1 CGCAGAG 10435 0.0 23.497272 8 CATGGGG 7430 0.0 22.234322 4 TACCTGG 2385 0.0 21.43496 2 TATACCG 180 5.3311214E-6 21.038015 5 GAGTACA 9345 0.0 19.809998 1 GTACCTG 2685 0.0 19.573372 1 CCTATAC 560 0.0 19.442019 3 GCAGAGT 12465 0.0 19.404783 9 >>END_MODULE