FastQCFastQC Report
Thu 26 May 2016
SRR936310_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936310_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3049187
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42540.13951259794823997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40600.040.6550141
GTATCAA70400.038.108081
ATCAACG85750.030.6490333
CAACGCA86700.030.3136985
TCAACGC87400.030.12474
AACGCAG89650.029.4220376
TATCAAC91750.028.8031582
TACATGG113250.025.3459242
GTACATG117550.025.3315851
ACGCAGA105700.024.8646897
CGCAGAG109700.023.91488
ACATGGG116500.023.5365643
ACCGTAT2257.25704E-821.084628
ATACCGT4000.020.1621696
TACCTGG25150.019.8080772
CATGGGG70500.019.7833294
GCAGAGT131450.019.5969059
GTACCTG28700.019.2259041
GAGTACA92400.019.0990871
GTGTAGC15200.018.4637171
Produced by FastQC (version 0.11.5)