##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936310_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3049187 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50945579920156 34.0 31.0 34.0 31.0 34.0 2 32.644530492882204 34.0 31.0 34.0 31.0 34.0 3 32.73059376155021 34.0 31.0 34.0 31.0 34.0 4 36.208410307403256 37.0 35.0 37.0 35.0 37.0 5 36.16542278318778 37.0 35.0 37.0 35.0 37.0 6 36.19860507079428 37.0 37.0 37.0 35.0 37.0 7 36.14942474830176 37.0 37.0 37.0 35.0 37.0 8 36.12556330589105 37.0 36.0 37.0 35.0 37.0 9 37.905300330875086 39.0 38.0 39.0 35.0 39.0 10-11 37.84050010707772 39.0 38.0 39.0 35.0 39.0 12-13 37.78884814214412 39.0 38.0 39.0 35.0 39.0 14-15 39.16750415766563 41.0 39.0 41.0 36.0 41.0 16-17 39.09123021972742 40.0 38.0 41.0 36.0 41.0 18-19 39.096024284506 40.0 38.5 41.0 36.0 41.0 20-21 39.03870244757045 40.0 39.0 41.0 35.0 41.0 22-23 38.926955611446594 40.0 38.0 41.0 35.0 41.0 24-25 39.078708357342464 40.0 39.0 41.0 35.0 41.0 26-27 39.004263759487365 40.0 39.0 41.0 35.0 41.0 28-29 38.91672845909417 40.0 38.0 41.0 35.0 41.0 30-31 38.81622134031137 40.0 38.0 41.0 35.0 41.0 32-33 38.683221461983145 40.0 38.0 41.0 35.0 41.0 34-35 38.55658951058102 40.0 38.0 41.0 34.0 41.0 36-37 38.421756356694424 40.0 38.0 41.0 34.0 41.0 38-39 38.29118417466688 40.0 38.0 41.0 34.0 41.0 40-41 38.15561918636017 40.0 38.0 41.0 33.0 41.0 42-43 38.19137265113619 40.0 38.0 41.0 34.0 41.0 44-45 38.07812853065424 40.0 38.0 41.0 33.0 41.0 46-47 37.91229531019252 40.0 37.5 41.0 33.0 41.0 48-49 37.705204862804415 40.0 37.0 41.0 33.0 41.0 50-51 37.50342255165066 40.0 37.0 41.0 33.0 41.0 52-53 37.27937086180677 40.0 36.0 41.0 32.0 41.0 54-55 37.07334020511041 39.5 36.0 41.0 32.0 41.0 56-57 36.81165881266055 39.0 35.0 41.0 31.0 41.0 58-59 36.537917320256184 39.0 35.0 41.0 31.0 41.0 60-61 36.24610560126355 38.0 35.0 40.0 31.0 41.0 62-63 35.935523960977136 38.0 35.0 40.0 30.5 41.0 64-65 35.55306201292345 37.0 35.0 40.0 30.0 41.0 66-67 35.20244183121599 36.5 34.0 39.5 29.0 41.0 68-69 34.84789470111213 36.0 34.0 39.0 29.0 41.0 70-71 34.478218620242046 35.5 34.0 39.0 29.0 40.5 72-73 34.0806628127432 35.0 34.0 37.5 28.5 40.0 74-75 33.66945402167857 35.0 33.5 37.0 28.0 39.0 76-77 32.9599194801762 35.0 32.5 36.0 27.0 39.0 78-79 33.0206638687624 35.0 33.0 36.0 27.0 39.0 80-81 32.87520312791574 35.0 33.0 36.0 27.0 37.0 82-83 32.6107611635495 35.0 33.0 35.5 27.0 37.0 84-85 32.335260677682285 35.0 33.0 35.0 26.5 36.5 86-87 31.952655576716023 35.0 33.0 35.0 26.0 36.0 88-89 31.476322869013938 35.0 32.0 35.0 24.5 36.0 90-91 31.270044113398097 35.0 32.0 35.0 24.0 35.5 92-93 31.000621969069133 34.0 32.0 35.0 23.5 35.0 94-95 30.85217043100341 34.0 32.0 35.0 23.0 35.0 96-97 30.613087521362253 34.0 32.0 35.0 21.0 35.0 98-99 30.17804122869473 34.0 31.0 35.0 10.0 35.0 100-101 26.793266205057282 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03717762403161373 1101 2 0.0881718575974304 1101 3 0.17074232671641454 1101 4 0.12303481483438361 1101 5 0.09817650324657023 1101 6 0.11021333535641276 1101 7 0.08585355872071432 1101 8 0.05160635237474054 1101 9 0.19493478928391994 1101 10-11 0.11267690722085177 1101 12-13 0.23022547197854948 1101 14-15 0.3473827632754052 1101 16-17 0.341634542278463 1101 18-19 0.2417946497383454 1101 20-21 0.16454301942126648 1101 22-23 0.1816084104235074 1101 24-25 0.16673310155544385 1101 26-27 0.2248638537132237 1101 28-29 0.21219928210591377 1101 30-31 0.21809471668689184 1101 32-33 0.2728959613177864 1101 34-35 0.23906518050247172 1101 36-37 0.161497074985526 1101 38-39 0.20476439779523758 1101 40-41 0.2704383071935297 1101 42-43 0.2435184781729518 1101 44-45 0.25044498105007307 1101 46-47 0.39766270753792554 1101 48-49 0.35439523402015993 1101 50-51 0.3017781681631746 1101 52-53 0.3796254191858779 1101 54-55 0.3654097334875601 1101 56-57 0.39730206457315376 1101 58-59 0.25296737786873535 1101 60-61 0.40367187419021633 1101 62-63 0.48073830837282827 1101 64-65 0.45438361241969005 1101 66-67 0.3736745213210355 1101 68-69 0.43209118990188955 1101 70-71 0.4166728638744459 1101 72-73 0.3575885767353313 1101 74-75 0.36234512996387735 1101 76-77 0.28796267260539565 1101 78-79 0.23918740597528654 1101 80-81 0.39391634743835624 1101 82-83 0.3417338464917634 1101 84-85 0.5430370824013053 1101 86-87 0.44785523801127525 1101 88-89 0.3828697136138963 1101 90-91 0.5566645823299261 1101 92-93 0.7640064480638991 1101 94-95 0.599685496604053 1101 96-97 0.5264780738965769 1101 98-99 0.5145819225452684 1101 100-101 0.645709762496427 1102 1 0.05101848289691446 1102 2 0.12907266311359678 1102 3 0.12560057317532625 1102 4 0.10882191983010614 1102 5 0.11821776890266023 1102 6 0.13590611885249615 1102 7 0.13611553844569357 1102 8 0.1559725344803482 1102 9 0.09013630030495534 1102 10-11 0.15992403042977799 1102 12-13 0.26218685178242396 1102 14-15 0.23840894951637637 1102 16-17 0.2716356567737961 1102 18-19 0.3570177027809862 1102 20-21 0.3195122021589114 1102 22-23 0.35590367543142776 1102 24-25 0.2841237013739004 1102 26-27 0.34593727402607755 1102 28-29 0.3194237048046631 1102 30-31 0.28395046579639427 1102 32-33 0.2408688849469982 1102 34-35 0.18541858110634735 1102 36-37 0.0725273525975112 1102 38-39 0.16718669474078496 1102 40-41 0.18641540431445947 1102 42-43 0.13924050250708575 1102 44-45 0.1467142079275945 1102 46-47 0.21316350454872435 1102 48-49 0.208116988529099 1102 50-51 0.2664605443258736 1102 52-53 0.13046437021114343 1102 54-55 0.05555172899145333 1102 56-57 0.1476224086589255 1102 58-59 0.03389249698042818 1102 60-61 0.043892617587935945 1102 62-63 0.12570694852720976 1102 64-65 0.12696541417736995 1102 66-67 0.014875434034053114 1102 68-69 0.03654220563240784 1102 70-71 0.11451558218721658 1102 72-73 0.09260044757705543 1102 74-75 0.2154806012707624 1102 76-77 0.1072508473699898 1102 78-79 0.12786875269625142 1102 80-81 0.16459566167177542 1102 82-83 0.2161201041777474 1102 84-85 0.2473500326904272 1102 86-87 0.07939178979204442 1102 88-89 0.17989071158580927 1102 90-91 0.35190942668918623 1102 92-93 0.3370602972521475 1102 94-95 0.3008296873704559 1102 96-97 0.07686015515794864 1102 98-99 0.26495190566653193 1102 100-101 0.7260408262675604 1103 1 -0.0018701256147366507 1103 2 0.048662922581989676 1103 3 0.1214958126715473 1103 4 0.0244717789499731 1103 5 0.11828028799786239 1103 6 0.08973930319613288 1103 7 0.11643758250892944 1103 8 0.10155150090674425 1103 9 0.04807792274620226 1103 10-11 0.15205737432440714 1103 12-13 0.1530559520732382 1103 14-15 0.164237681142545 1103 16-17 0.22238348133559782 1103 18-19 0.06812912821635564 1103 20-21 0.06463684430073613 1103 22-23 0.14342998437194154 1103 24-25 0.15980377334158646 1103 26-27 0.17535463000584883 1103 28-29 0.16891930866557203 1103 30-31 0.2722879670694027 1103 32-33 0.19394773821666433 1103 34-35 0.140220669979648 1103 36-37 0.2418638580912713 1103 38-39 0.2249428815851786 1103 40-41 0.17853828121126014 1103 42-43 0.1765542182347346 1103 44-45 0.2134274574495052 1103 46-47 0.27283628356494205 1103 48-49 0.2086314273282781 1103 50-51 0.3167179758943419 1103 52-53 0.278046635156322 1103 54-55 0.27308352603353114 1103 56-57 0.28928869813647395 1103 58-59 0.2560942724095696 1103 60-61 0.19094813793917353 1103 62-63 0.34154224324392146 1103 64-65 0.2766641343747125 1103 66-67 0.2859623327474594 1103 68-69 0.2952281164875288 1103 70-71 0.1604483048513785 1103 72-73 0.15435234012342391 1103 74-75 0.2541592700949451 1103 76-77 0.23756355600271917 1103 78-79 0.16759794034405928 1103 80-81 0.2298213244873466 1103 82-83 0.3399944775723327 1103 84-85 0.30633170695744383 1103 86-87 0.29239463002604893 1103 88-89 0.443369800221479 1103 90-91 0.5877812427416025 1103 92-93 0.5701703817802297 1103 94-95 0.6186853955618759 1103 96-97 0.6377161292676874 1103 98-99 0.4162097842040957 1103 100-101 0.8729680551723114 1104 1 0.048319446291472445 1104 2 0.08332890453168318 1104 3 0.012199687149816896 1104 4 0.03544564315748744 1104 5 0.024615923145695717 1104 6 0.02428072493079725 1104 7 0.03893162765654523 1104 8 0.0034264599329887346 1104 9 -0.0652822529492596 1104 10-11 0.015186458041981155 1104 12-13 0.11622491691229442 1104 14-15 0.13755639139800735 1104 16-17 0.14954990033867688 1104 18-19 0.23462516195687755 1104 20-21 0.2018049568856881 1104 22-23 0.2231963247054196 1104 24-25 0.1741948487114371 1104 26-27 0.253591010329707 1104 28-29 0.2006805245480905 1104 30-31 0.099879693648802 1104 32-33 0.1257223388591342 1104 34-35 0.07504890799891939 1104 36-37 0.15355339721847372 1104 38-39 0.12093537519327668 1104 40-41 0.14965178397562084 1104 42-43 0.21054768428661674 1104 44-45 0.13478680274353394 1104 46-47 0.13507459785003562 1104 48-49 0.15932661537049597 1104 50-51 0.20303110279279224 1104 52-53 0.20381830754110553 1104 54-55 0.0859987267503044 1104 56-57 0.04870282807245019 1104 58-59 0.1039185660220312 1104 60-61 -0.001528261177980994 1104 62-63 0.04546944107613626 1104 64-65 -0.05163998922398605 1104 66-67 -0.026196765611402384 1104 68-69 0.09372301388924598 1104 70-71 0.12775698473162578 1104 72-73 -0.06489330573222674 1104 74-75 -0.022458373750247063 1104 76-77 0.08829889832478699 1104 78-79 0.08391505535465882 1104 80-81 0.1743360313337874 1104 82-83 0.035722280723490485 1104 84-85 0.17646757592884654 1104 86-87 0.20363946733292337 1104 88-89 0.338980964750224 1104 90-91 0.40334416750286195 1104 92-93 0.3956175420258532 1104 94-95 0.18572310234275946 1104 96-97 0.18414103256962733 1104 98-99 0.2274674599541875 1104 100-101 0.26521418178667844 1105 1 -0.015718785426159343 1105 2 0.06978976957690008 1105 3 0.05335377349311443 1105 4 0.04188555917291836 1105 5 -0.04609398827896172 1105 6 0.013005702696659682 1105 7 0.0198415494457862 1105 8 0.04491059033625078 1105 9 0.06592740433361399 1105 10-11 0.10322093442878355 1105 12-13 0.10446804605452797 1105 14-15 0.11681727068144454 1105 16-17 0.17462408827586984 1105 18-19 0.11356213479589883 1105 20-21 0.10134488436498401 1105 22-23 -0.012648423196928604 1105 24-25 0.14070721999818403 1105 26-27 0.1364000464701789 1105 28-29 0.026797819223261854 1105 30-31 0.19339320242285396 1105 32-33 0.10541304752995018 1105 34-35 0.06510060530481354 1105 36-37 0.059670650016521165 1105 38-39 0.137006112979158 1105 40-41 0.18423535003232416 1105 42-43 0.22981050562153627 1105 44-45 0.21591973328876435 1105 46-47 0.1504923142801573 1105 48-49 0.2106597866044737 1105 50-51 0.18998997841303122 1105 52-53 0.2644493774334862 1105 54-55 0.2654377408372426 1105 56-57 0.21241835731289171 1105 58-59 0.09612857088501414 1105 60-61 0.21511602303994692 1105 62-63 0.23465646743935764 1105 64-65 0.08830680589377238 1105 66-67 0.002187939607694034 1105 68-69 0.05960892961886799 1105 70-71 0.009678356772681695 1105 72-73 0.058105295048470396 1105 74-75 0.14274752296716287 1105 76-77 -0.010704177549754945 1105 78-79 -0.003084467246161182 1105 80-81 0.047548139998632166 1105 82-83 0.15493505889880765 1105 84-85 0.16867535606976958 1105 86-87 0.12374858602368377 1105 88-89 0.2812127849759314 1105 90-91 0.37483533587976936 1105 92-93 0.36013237464038284 1105 94-95 0.16456697710713186 1105 96-97 0.1958136911543562 1105 98-99 0.17382541982648192 1105 100-101 0.42910389367732193 1106 1 0.07000093119882678 1106 2 0.07893418741100788 1106 3 0.04158804693707907 1106 4 0.039397548052818365 1106 5 0.0793971039886543 1106 6 0.03747251545109265 1106 7 0.08223646218201708 1106 8 0.11146681644085277 1106 9 0.045486836789358165 1106 10-11 0.1323782672070024 1106 12-13 0.07873490608280775 1106 14-15 0.1604091275891264 1106 16-17 0.02155386130698389 1106 18-19 0.1342563977758786 1106 20-21 0.1443983656645642 1106 22-23 0.032439680320919706 1106 24-25 0.07434360513416038 1106 26-27 0.045273430062870545 1106 28-29 0.1602149968324511 1106 30-31 0.14939095583973483 1106 32-33 0.14107439270510014 1106 34-35 0.18459197757326962 1106 36-37 0.1992334920579779 1106 38-39 0.2581982311197848 1106 40-41 0.17536195379281594 1106 42-43 0.1389764104220106 1106 44-45 0.19059881678301593 1106 46-47 0.2004835310558235 1106 48-49 0.20544777449234886 1106 50-51 0.2928505778298387 1106 52-53 0.20115489558745026 1106 54-55 0.2812975628533252 1106 56-57 0.2355231695713087 1106 58-59 0.25320746396857885 1106 60-61 0.2784131607205609 1106 62-63 0.20882461894940008 1106 64-65 0.21039893578330293 1106 66-67 0.16107564573681543 1106 68-69 0.2402925009676764 1106 70-71 0.1738359342670961 1106 72-73 0.07375638111408023 1106 74-75 0.16895238974607452 1106 76-77 0.1850086351700284 1106 78-79 0.11978032080116918 1106 80-81 0.10591914718848017 1106 82-83 0.2762279722848291 1106 84-85 0.38108508456885914 1106 86-87 0.36850188997674493 1106 88-89 0.3070777681638681 1106 90-91 0.5048503894030567 1106 92-93 0.5404765050373079 1106 94-95 0.4680882090501477 1106 96-97 0.4855701119189888 1106 98-99 0.5435758940510382 1106 100-101 0.8790094787583094 1107 1 0.025020831430808244 1107 2 0.07204535523340638 1107 3 0.07091243230643585 1107 4 0.012586102502559982 1107 5 0.026086492840363462 1107 6 0.057070002749654236 1107 7 0.04214040497465987 1107 8 -0.008046614841731525 1107 9 0.028150929110857703 1107 10-11 0.09464861132547497 1107 12-13 0.0659206346506167 1107 14-15 0.05823114046517475 1107 16-17 0.134288645704693 1107 18-19 0.08304500186198283 1107 20-21 0.10385660100207161 1107 22-23 0.046262438536906814 1107 24-25 0.15229903756706875 1107 26-27 0.08663694922219634 1107 28-29 0.15958871528280127 1107 30-31 0.07783958002670488 1107 32-33 0.01150761293516922 1107 34-35 0.08914820564464065 1107 36-37 0.1341425898536457 1107 38-39 0.19179364741160754 1107 40-41 -0.008180455692027522 1107 42-43 -0.027991985601936165 1107 44-45 0.0509423353251961 1107 46-47 0.07480316667093945 1107 48-49 -0.012970200437052881 1107 50-51 0.15284844298978584 1107 52-53 0.10227068059740674 1107 54-55 0.3120457488021344 1107 56-57 0.1681155951005593 1107 58-59 0.10861058917840438 1107 60-61 0.23523873568053233 1107 62-63 0.2479143745380341 1107 64-65 0.1701305816218195 1107 66-67 0.11295645703430068 1107 68-69 0.22511686071100456 1107 70-71 0.29065605028743136 1107 72-73 0.2631477490764098 1107 74-75 0.23910062472203464 1107 76-77 0.16410806110729936 1107 78-79 0.09728139782267675 1107 80-81 0.18938179152691248 1107 82-83 0.38512626900826774 1107 84-85 0.2858128812060059 1107 86-87 0.3654356398775782 1107 88-89 0.4472160454088687 1107 90-91 0.19736594744719937 1107 92-93 0.4504363814337786 1107 94-95 0.5253947587049765 1107 96-97 0.237261131698439 1107 98-99 0.17510011819494053 1107 100-101 0.2938702830818123 1108 1 -0.08949754501017537 1108 2 -0.040845270013214474 1108 3 -0.03597694160092857 1108 4 0.0058018486688311555 1108 5 -0.037569678023636754 1108 6 -0.06026950604601922 1108 7 -5.340517313356941E-4 1108 8 -0.06629854062254026 1108 9 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-0.4543817179152967 2316 66-67 -0.439258746095021 2316 68-69 -0.30606106168921343 2316 70-71 -0.3559979014616559 2316 72-73 -0.3446720555511149 2316 74-75 -0.4102988965556378 2316 76-77 -0.3488390282911311 2316 78-79 -0.501655030257929 2316 80-81 -0.6297212473377627 2316 82-83 -0.6585163249540713 2316 84-85 -0.4657037213213471 2316 86-87 -0.5600608944401309 2316 88-89 -0.4953919170499468 2316 90-91 -0.5376097040658827 2316 92-93 -0.5539008644253407 2316 94-95 -0.44404987759991243 2316 96-97 -0.44635673119380215 2316 98-99 -0.6500712855837172 2316 100-101 -0.5612675297078589 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 24.0 5 51.0 6 80.0 7 192.0 8 465.0 9 883.0 10 1397.0 11 2149.0 12 3270.0 13 4688.0 14 5972.0 15 7624.0 16 9497.0 17 12359.0 18 11892.0 19 8736.0 20 8323.0 21 9085.0 22 9918.0 23 11578.0 24 14030.0 25 17173.0 26 21363.0 27 26922.0 28 34097.0 29 42504.0 30 53051.0 31 66436.0 32 83370.0 33 108937.0 34 152652.0 35 234679.0 36 412657.0 37 752050.0 38 782209.0 39 138791.0 40 73.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.71344466546594 17.833716056036785 12.88210748616131 24.570731792335966 2 16.390888359973026 19.081744180248926 38.866339257832486 25.661028201945555 3 20.143139794312386 22.687260571424446 28.156226561375213 29.013373072887955 4 13.320895045138261 14.869930902893133 35.10611189146484 36.70306216050377 5 14.599366978804515 36.75960182173149 33.46774074531998 15.173290454144006 6 35.387170416245375 34.12981886647162 16.171064614928504 14.311946102354497 7 31.16512696663078 30.86117709409098 19.295995949084134 18.677699990194107 8 27.81915310540154 34.6986262239738 18.550977686839147 18.931242983785516 9 26.94009911494441 15.649220595522676 18.93567039345242 38.4750098960805 10-11 26.465603768359248 25.426684597179204 26.22844152941522 21.879270105046324 12-13 26.527297207729738 22.2145225339685 27.159859575614536 24.09832068268723 14-15 23.271563862760793 24.048311894285263 24.544001401029192 28.13612284192475 16-17 23.832464489909295 27.29066957106503 25.222449989234768 23.654415949790902 18-19 23.723225240039394 26.079509062579632 26.839022992030337 23.35824270535064 20-21 24.20054919557246 25.391440406901904 26.941410940030902 23.466599457494734 22-23 23.845036019449125 25.2462624451247 26.808613839890384 24.100087695535795 24-25 23.72065947112436 25.44609738579454 27.02226223389776 23.810980909183336 26-27 23.535618010564065 25.78953763728799 26.804718950001977 23.87012540214597 28-29 23.47596041739297 25.97668439748294 26.863580505438133 23.683774679685953 30-31 23.637973324377835 25.662750057311033 26.772632775484063 23.92664384282707 32-33 23.42701932791202 25.606257109587805 26.862152420379587 24.104571142120587 34-35 23.845060849274343 25.81004597209206 26.713991602529575 23.630901576104023 36-37 24.030888899529252 25.490475162879534 26.710718860705775 23.767917076885436 38-39 23.70089360016988 25.73683274123474 26.643108439721136 23.919165218874237 40-41 23.904519402695296 25.569418371724183 26.79962442164475 23.726437803935767 42-43 23.913041695821725 25.49032565826208 26.56014349291038 24.03648915300581 44-45 23.698317959492165 25.785236750485275 26.615868572147296 23.900576717875268 46-47 23.89856529231179 25.93899872389884 26.35361269796911 23.808823285820253 48-49 24.05385171099232 25.939793209823392 26.25538234206398 23.750972737120303 50-51 23.98428326850291 26.07452328319635 26.167652788801497 23.773540659499243 52-53 24.207830654026044 25.799655521148484 26.16984811345926 23.822665711366213 54-55 23.998983331556897 25.853714529339904 26.279895381537628 23.867406757565572 56-57 23.775215334951085 26.206664486779157 26.086136604170363 23.931983574099394 58-59 23.94097823768061 25.820159838171108 26.25946309893605 23.979398825212236 60-61 23.82774515478929 25.983169052308106 26.36870335782654 23.820382435076063 62-63 23.76226843417606 26.045385212517303 26.44803352500191 23.744312828304725 64-65 23.743725123300212 25.987602829431744 26.355998948564093 23.91267309870396 66-67 23.65097647340094 26.14675649607584 26.202951803218365 23.99931522730485 68-69 23.810691177681132 25.83773969913948 26.566114180599616 23.785454942579776 70-71 23.820618893448213 25.75890795701407 26.322058622897142 24.09841452664057 72-73 23.754185184128424 26.09501864290659 26.132898064345166 24.017898108619814 74-75 23.683116188019955 26.26688687837119 26.084887545434242 23.965109388174618 76-77 23.82167160159473 26.072833426380086 26.12705136870731 23.978443603317874 78-79 23.73312037767347 26.110421230344212 26.2313988675377 23.925059524444624 80-81 23.813749024524668 26.21156127050958 26.061585268965544 23.91310443600021 82-83 23.917522933162182 26.23933855155489 26.03516937465626 23.807969140626664 84-85 23.78027223199539 26.318787707056757 25.844608555000647 24.056331505947202 86-87 23.833608308321804 26.103206822947904 26.0634258857169 23.999758983013393 88-89 23.689183908411913 26.220610652143183 25.95414294275431 24.136062496690595 90-91 23.586492705648503 26.364019631888112 26.031184876267684 24.018302786195697 92-93 23.65872403566199 26.168299614937457 26.123384149264734 24.049592200135816 94-95 23.640843326670197 25.996116906053068 26.207752085441182 24.15528768183555 96-97 23.743266856799526 25.768129307834194 26.220984842720597 24.26761899264569 98-99 23.41350466285898 25.97787212271182 26.07606519311468 24.53255802131452 100-101 23.39599040851696 25.979141492412587 26.155944081056987 24.468924018013468 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 122.0 1 90.0 2 121.0 3 205.5 4 171.0 5 162.0 6 358.0 7 677.0 8 1012.5 9 1085.5 10 874.5 11 743.5 12 774.5 13 873.0 14 949.0 15 1057.0 16 1181.5 17 1300.5 18 1429.0 19 1560.5 20 1771.5 21 2151.0 22 2640.0 23 3300.0 24 4259.5 25 5448.0 26 7218.5 27 9942.0 28 12992.0 29 16769.5 30 21513.0 31 28152.5 32 36547.0 33 41392.5 34 44208.0 35 49544.0 36 56834.0 37 63138.5 38 67923.5 39 72057.5 40 75813.0 41 80072.0 42 83549.5 43 88899.0 44 96023.0 45 103559.5 46 108664.0 47 112998.5 48 118836.0 49 120114.5 50 118849.5 51 119773.0 52 121432.0 53 123570.0 54 126546.5 55 121430.0 56 110532.0 57 101271.0 58 96135.5 59 89250.0 60 76845.0 61 65674.0 62 55405.5 63 42748.0 64 31580.0 65 24136.5 66 17168.0 67 13540.5 68 10814.5 69 7948.0 70 6154.0 71 4459.5 72 3541.5 73 2968.0 74 2266.5 75 1658.0 76 1075.5 77 584.0 78 364.0 79 204.0 80 111.0 81 62.5 82 34.5 83 15.0 84 7.5 85 4.5 86 3.5 87 3.0 88 2.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2707606978515913 2 0.019775763178840788 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.787628308791819E-4 12-13 4.9193440743385034E-5 14-15 0.0 16-17 6.067191025017488E-4 18-19 0.0 20-21 0.0 22-23 3.279562716225669E-5 24-25 0.0025252632914937656 26-27 0.002508865477912637 28-29 0.002295693901357969 30-31 0.001180642577841241 32-33 0.001557792290207193 34-35 0.0017873616803429897 36-37 3.279562716225669E-5 38-39 1.4758032223015512E-4 40-41 7.54299424731904E-4 42-43 0.003820690564402905 44-45 0.004017464327376445 46-47 0.01467604315510987 48-49 0.014577656273623101 50-51 0.006788694822587135 52-53 0.007657778942386938 54-55 3.9354752594708027E-4 56-57 0.0032795627162256692 58-59 0.001328222900071396 60-61 0.0013774163408147811 62-63 0.0 64-65 7.706972383130323E-4 66-67 0.0 68-69 0.0 70-71 9.838688148677007E-5 72-73 0.0012462338321657543 74-75 0.0 76-77 0.19395333903758608 78-79 0.1039457402907726 80-81 0.0010658578827733424 82-83 0.0 84-85 7.54299424731904E-4 86-87 0.12326236468934179 88-89 0.5919774680923144 90-91 0.31288668094150995 92-93 0.2961937067159213 94-95 0.002361285155682482 96-97 0.002984402071765359 98-99 0.003017197698927616 100-101 7.706972383130323E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3049187.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.89641560250156 #Duplication Level Percentage of deduplicated Percentage of total 1 64.25102989069606 24.99134761517265 2 15.649482321448529 12.174175366781258 3 6.906276742547042 8.058882313320014 4 3.7142924617623856 5.7789065304779355 5 2.3123905056160043 4.497185107085941 6 1.5368042442267615 3.5865705949879456 7 1.0646049253512428 2.8986520940252465 8 0.7452436396792854 2.318984506726913 9 0.5805474183108054 2.0323092293618767 >10 2.958018225728628 20.216876680109827 >50 0.1840427029710523 4.913693138966385 >100 0.091122428865778 6.158962843107404 >500 0.0045452412467629 1.2432845697981911 >1k 0.0015992515497020826 1.1301694100784294 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4254 0.13951259794823997 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 3.279562716225669E-5 6 0.0 0.0 0.0 0.0 3.279562716225669E-5 7 0.0 0.0 0.0 0.0 3.279562716225669E-5 8 0.0 0.0 0.0 0.0 3.279562716225669E-5 9 0.0 0.0 0.0 0.0 3.279562716225669E-5 10-11 0.0 0.0 0.0 0.0 3.279562716225669E-5 12-13 0.0 0.0 0.0 3.279562716225669E-5 1.6397813581128345E-4 14-15 0.0 0.0 0.0 3.279562716225669E-5 2.2956939013579685E-4 16-17 0.0 0.0 0.0 1.6397813581128345E-4 2.6236501729805353E-4 18-19 0.0 0.0 0.0 2.787628308791819E-4 2.6236501729805353E-4 20-21 0.0 0.0 0.0 5.411278481772354E-4 4.5913878027159375E-4 22-23 0.0 0.0 0.0 0.0010986535099355992 4.919344074338504E-4 24-25 0.0 0.0 0.0 0.0016561791716939631 4.919344074338504E-4 26-27 0.0 0.0 0.0 0.0019349420025731449 5.575256617583637E-4 28-29 0.0 0.0 0.0 0.002459672037169252 5.903212889206205E-4 30-31 0.0 0.0 0.0 0.004296227158255627 5.903212889206205E-4 32-33 0.0 0.0 0.0 0.007657778942386938 6.231169160828771E-4 34-35 0.0 0.0 0.0 0.011954006100642565 6.559125432451339E-4 36-37 0.0 1.6397813581128346E-5 0.0 0.02089081450235751 6.559125432451339E-4 38-39 0.0 3.279562716225669E-5 0.0 0.03423863475739599 6.559125432451339E-4 40-41 0.0 3.279562716225669E-5 0.0 0.05040687894838854 6.559125432451339E-4 42-43 0.0 3.279562716225669E-5 0.0 0.06716544442830172 6.559125432451339E-4 44-45 0.0 3.279562716225669E-5 0.0 0.08643287538612751 7.215037975696473E-4 46-47 0.0 3.279562716225669E-5 0.0 0.10955379253551849 8.034928654752889E-4 48-49 0.0 3.279562716225669E-5 0.0 0.13713491497897634 8.198906790564173E-4 50-51 0.0 3.279562716225669E-5 0.0 0.1671265160188601 8.526863062186741E-4 52-53 0.0 3.279562716225669E-5 0.0 0.1944124778178577 9.510731877054442E-4 54-55 0.0 3.279562716225669E-5 0.0 0.22819197379498207 9.838688148677008E-4 56-57 0.0 3.279562716225669E-5 0.0 0.26566097782786036 0.001000266628448829 58-59 0.0 3.279562716225669E-5 0.0 0.3091479794450127 0.0010166644420299576 60-61 0.0 3.279562716225669E-5 0.0 0.3572099710512999 0.0010166644420299576 62-63 0.0 3.279562716225669E-5 0.0 0.40561631674279075 0.001082255696354471 64-65 0.0 3.279562716225669E-5 0.0 0.4583188895925373 0.0010986535099355992 66-67 0.0 3.279562716225669E-5 0.0 0.5223851472540058 0.0011150513235167277 68-69 0.0 3.279562716225669E-5 0.0 0.5943223554344158 0.0011150513235167277 70-71 0.0 3.279562716225669E-5 0.0 0.6729006781151828 0.0011150513235167277 72-73 0.0 3.279562716225669E-5 0.0 0.7577101699567786 0.001229836018584626 74-75 0.0 3.279562716225669E-5 0.0 0.8454056769886531 0.0013446207136525244 76-77 0.0 3.279562716225669E-5 0.0 0.9414640689469029 0.0013938141543959094 78-79 0.0 3.279562716225669E-5 0.0 1.0547401651653376 0.001459405408720423 80-81 0.0 3.279562716225669E-5 0.0 1.182544724216652 0.0015249966630449363 82-83 0.0 3.279562716225669E-5 0.0 1.3179906643967718 0.0016397813581128346 84-85 0.0 3.279562716225669E-5 0.0 1.4554863312745332 0.0016397813581128346 86-87 0.0 3.279562716225669E-5 0.0 1.6040341245059748 0.0016561791716939631 88-89 0.0 3.279562716225669E-5 0.0 1.7693732788444918 0.0016889747988562197 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4060 0.0 40.655014 1 GTATCAA 7040 0.0 38.10808 1 ATCAACG 8575 0.0 30.649033 3 CAACGCA 8670 0.0 30.313698 5 TCAACGC 8740 0.0 30.1247 4 AACGCAG 8965 0.0 29.422037 6 TATCAAC 9175 0.0 28.803158 2 TACATGG 11325 0.0 25.345924 2 GTACATG 11755 0.0 25.331585 1 ACGCAGA 10570 0.0 24.864689 7 CGCAGAG 10970 0.0 23.9148 8 ACATGGG 11650 0.0 23.536564 3 ACCGTAT 225 7.25704E-8 21.08462 8 ATACCGT 400 0.0 20.162169 6 TACCTGG 2515 0.0 19.808077 2 CATGGGG 7050 0.0 19.783329 4 GCAGAGT 13145 0.0 19.596905 9 GTACCTG 2870 0.0 19.225904 1 GAGTACA 9240 0.0 19.099087 1 GTGTAGC 1520 0.0 18.463717 1 >>END_MODULE