FastQCFastQC Report
Thu 26 May 2016
SRR936309_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936309_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2321778
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45480.19588436103710172No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30580.13170940546426058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58900.037.7042581
GGTATCA39200.033.580841
ATCAACG70100.030.6610093
TCAACGC71250.030.166134
GTACATG91700.029.7947181
CAACGCA73000.029.4429685
TATCAAC74850.028.9676282
AACGCAG74650.028.9177786
TACATGG93250.028.6839452
ACATGGG92700.027.9865573
CATGGGG47900.024.6100464
ACGCAGA88500.024.3927547
CGCAGAG90750.023.6846628
GTACCTG20000.023.2021851
TACCTGG19400.021.9624122
CATGGGA49050.021.427064
AGTACAT63100.021.3823172
GCAGAGT102350.020.3522979
GAGTACA77700.019.988751
ATACCGT1951.0638929E-519.4216126
Produced by FastQC (version 0.11.5)