##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936309_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2321778 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.71528802495329 33.0 31.0 34.0 30.0 34.0 2 31.80562741140626 34.0 31.0 34.0 30.0 34.0 3 31.8775968245026 34.0 31.0 34.0 30.0 34.0 4 35.36274053763969 37.0 35.0 37.0 33.0 37.0 5 35.35259787972838 37.0 35.0 37.0 33.0 37.0 6 35.44299713409293 37.0 35.0 37.0 33.0 37.0 7 35.38548603699406 37.0 35.0 37.0 33.0 37.0 8 35.386207897568156 37.0 35.0 37.0 33.0 37.0 9 37.05985068339867 39.0 37.0 39.0 33.0 39.0 10-11 36.97844475225452 39.0 37.0 39.0 33.0 39.0 12-13 36.87775553907393 39.0 37.0 39.0 33.0 39.0 14-15 38.13204277066972 40.0 38.0 41.0 33.0 41.0 16-17 38.08013061541629 40.0 38.0 41.0 33.0 41.0 18-19 38.025222480357726 40.0 38.0 41.0 33.0 41.0 20-21 37.950675086076274 40.0 38.0 41.0 33.0 41.0 22-23 37.78903818539068 40.0 38.0 41.0 32.5 41.0 24-25 37.946396899272884 40.0 38.0 41.0 33.0 41.0 26-27 37.91183868569691 40.0 38.0 41.0 33.0 41.0 28-29 37.7811892437606 40.0 38.0 41.0 32.5 41.0 30-31 37.64266587933902 40.0 38.0 41.0 32.0 41.0 32-33 37.47759109613408 40.0 37.5 41.0 32.0 41.0 34-35 37.22780967861699 40.0 37.0 41.0 31.5 41.0 36-37 37.04387779537923 40.0 37.0 41.0 31.0 41.0 38-39 36.893666621012 40.0 37.0 41.0 30.5 41.0 40-41 36.72358812944218 40.0 36.5 41.0 30.0 41.0 42-43 36.551515045796805 40.0 36.0 41.0 30.0 41.0 44-45 36.38269248825684 40.0 36.0 41.0 30.0 41.0 46-47 36.11747484040248 40.0 36.0 41.0 29.0 41.0 48-49 35.95915350218668 39.5 35.5 41.0 28.0 41.0 50-51 35.65719870719767 39.5 35.5 40.5 28.0 41.0 52-53 35.68067274304434 39.0 35.0 40.5 28.0 41.0 54-55 35.76216352295525 39.0 35.0 41.0 28.0 41.0 56-57 35.614299472214825 39.0 35.0 41.0 28.0 41.0 58-59 35.39496648689065 39.0 35.0 41.0 27.5 41.0 60-61 35.11037338625829 38.5 35.0 41.0 27.0 41.0 62-63 34.810527320010785 38.0 34.5 40.0 26.0 41.0 64-65 34.48195930015704 37.5 34.0 40.0 26.0 41.0 66-67 34.133953375387314 37.0 34.0 40.0 26.0 41.0 68-69 33.75586274829032 36.0 34.0 39.0 25.5 41.0 70-71 33.351610059187394 36.0 33.5 39.0 24.5 41.0 72-73 32.94054448788816 35.0 33.0 38.5 23.5 40.0 74-75 32.51941636969599 35.0 33.0 37.5 22.5 39.5 76-77 32.10699666376372 35.0 32.5 37.0 21.5 39.0 78-79 31.71607061484776 35.0 32.0 36.5 20.0 39.0 80-81 31.31659249936902 35.0 32.0 36.0 18.5 37.5 82-83 30.967526611071342 35.0 31.5 35.5 18.0 37.0 84-85 30.64966697935806 35.0 31.0 35.0 14.5 37.0 86-87 30.330214387422053 34.5 31.0 35.0 8.5 36.0 88-89 30.016077979892998 34.0 31.0 35.0 6.0 36.0 90-91 29.676445594712327 34.0 30.5 35.0 2.0 36.0 92-93 29.37063211900535 34.0 30.0 35.0 2.0 35.0 94-95 29.008451712437623 34.0 30.0 35.0 2.0 35.0 96-97 28.609601348621617 34.0 29.5 35.0 2.0 35.0 98-99 28.132649202464663 34.0 29.0 35.0 2.0 35.0 100-101 24.887765324677897 30.5 23.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29133978857961296 1101 2 0.2344040187051597 1101 3 0.3089636240267133 1101 4 0.13218976535631555 1101 5 0.4029982542607584 1101 6 0.27743082255232565 1101 7 0.396654860832129 1101 8 0.46012829100120456 1101 9 0.45542541058188846 1101 10-11 0.5501366108816583 1101 12-13 0.5185700080092843 1101 14-15 0.659570086487733 1101 16-17 0.6531625919033317 1101 18-19 0.4883876361353998 1101 20-21 0.5894497070838653 1101 22-23 0.6316075123757301 1101 24-25 0.6310602513048948 1101 26-27 0.5903674979691473 1101 28-29 0.5029123977128833 1101 30-31 0.6851749930691042 1101 32-33 0.6185282757010455 1101 34-35 0.4480898651915197 1101 36-37 0.42226361354810393 1101 38-39 0.45720568278105134 1101 40-41 0.4144889975214383 1101 42-43 0.4690328076283876 1101 44-45 0.5698101291385242 1101 46-47 0.5038165709332887 1101 48-49 0.5690035331549268 1101 50-51 0.6949563076272298 1101 52-53 0.8031914844225767 1101 54-55 0.7860881745027655 1101 56-57 0.707681793005257 1101 58-59 0.7538048156061308 1101 60-61 0.6639339257007677 1101 62-63 0.7375957067518399 1101 64-65 0.6499682368176352 1101 66-67 0.7392399300380319 1101 68-69 0.681790118836112 1101 70-71 0.8236099057936173 1101 72-73 0.8459179438728768 1101 74-75 0.65040412827355 1101 76-77 0.5138635978332289 1101 78-79 0.7131460855974119 1101 80-81 0.56036676416117 1101 82-83 0.8240764857631184 1101 84-85 0.7849856903588233 1101 86-87 0.6531370985045442 1101 88-89 0.5861993378040928 1101 90-91 0.709210917738293 1101 92-93 0.7090777289901489 1101 94-95 0.9505349650164057 1101 96-97 0.9505636482692559 1101 98-99 1.1380977739512268 1101 100-101 1.2310798012518056 1102 1 0.28790096941720833 1102 2 0.2682607005338049 1102 3 0.34919276380517417 1102 4 0.29764261293234284 1102 5 0.34926335215329374 1102 6 0.33483141976263653 1102 7 0.23471416108472454 1102 8 0.3327787900913606 1102 9 0.33964338373326086 1102 10-11 0.48013947758958153 1102 12-13 0.5276646081404479 1102 14-15 0.5774357055551533 1102 16-17 0.5812271703434746 1102 18-19 0.5026724540256282 1102 20-21 0.49738421326574667 1102 22-23 0.49282169236807505 1102 24-25 0.6731434005988675 1102 26-27 0.5863042778318146 1102 28-29 0.5070655561975457 1102 30-31 0.45368599052351755 1102 32-33 0.39664311380587236 1102 34-35 0.3568459969321296 1102 36-37 0.38559398994808447 1102 38-39 0.47936398165441574 1102 40-41 0.44164410364500384 1102 42-43 0.4083629330376368 1102 44-45 0.513596693505967 1102 46-47 0.6206611252340295 1102 48-49 0.693851862159498 1102 50-51 0.633577015101217 1102 52-53 0.662156344489091 1102 54-55 0.6048412426799743 1102 56-57 0.6078533033420115 1102 58-59 0.6328452435699461 1102 60-61 0.5446312616309541 1102 62-63 0.7075738927039836 1102 64-65 0.6563863406318333 1102 66-67 0.6760825403909081 1102 68-69 0.8034356091846675 1102 70-71 0.844866606569596 1102 72-73 0.7911477049764528 1102 74-75 0.926627554321712 1102 76-77 0.8257619121351496 1102 78-79 0.8389497788522782 1102 80-81 0.7640704769785494 1102 82-83 0.7912862684961866 1102 84-85 0.933445234712206 1102 86-87 0.7755887120427865 1102 88-89 0.7947674725615101 1102 90-91 0.7296248309914652 1102 92-93 0.7228170111320331 1102 94-95 0.7848570870200504 1102 96-97 0.8493526179645876 1102 98-99 0.7037725760807056 1102 100-101 0.8017170839571897 1103 1 0.3120718929652284 1103 2 0.41440113464740236 1103 3 0.4016169787593391 1103 4 0.26104116416031786 1103 5 0.1941420854843514 1103 6 0.17386891524478898 1103 7 0.2535201779741101 1103 8 0.19520431183648412 1103 9 0.2876262753673302 1103 10-11 0.4084704700796564 1103 12-13 0.3840489883389111 1103 14-15 0.42559142323651145 1103 16-17 0.4588775131112044 1103 18-19 0.4292403382839751 1103 20-21 0.5208698367992781 1103 22-23 0.57260033846201 1103 24-25 0.6248883197132145 1103 26-27 0.5867463499078127 1103 28-29 0.495443239975323 1103 30-31 0.4862214357566117 1103 32-33 0.5919443173485703 1103 34-35 0.34854740343947554 1103 36-37 0.29377344177871123 1103 38-39 0.3632063944378956 1103 40-41 0.4794078899952794 1103 42-43 0.3544174250432519 1103 44-45 0.4051631826766666 1103 46-47 0.3748739955944842 1103 48-49 0.32798736107697124 1103 50-51 0.35928665556888717 1103 52-53 0.39656921273041945 1103 54-55 0.4350381207446077 1103 56-57 0.4932265452078255 1103 58-59 0.5721193741713364 1103 60-61 0.47475173362971645 1103 62-63 0.39439979165255323 1103 64-65 0.39673992765528254 1103 66-67 0.33506778778055946 1103 68-69 0.30648902632528774 1103 70-71 0.3666982565756598 1103 72-73 0.2907875722253266 1103 74-75 0.3054531848483535 1103 76-77 0.3269316666350406 1103 78-79 0.29443812427833294 1103 80-81 0.30783019697209824 1103 82-83 0.4096623393878289 1103 84-85 0.5103830820860864 1103 86-87 0.4879038629300503 1103 88-89 0.49064860873972194 1103 90-91 0.417540781964437 1103 92-93 0.5638093952485193 1103 94-95 0.5833634602748639 1103 96-97 0.6911028231072187 1103 98-99 0.6406491573272 1103 100-101 0.7784055814890003 1104 1 0.28983490595734906 1104 2 0.19717299316924297 1104 3 0.27671764168804813 1104 4 0.29096707212792694 1104 5 0.29737896614003034 1104 6 0.2252690476704302 1104 7 0.23896319206406247 1104 8 0.20185281095535856 1104 9 0.24027141467155388 1104 10-11 0.33515698153309614 1104 12-13 0.38404841655289346 1104 14-15 0.5010088556199577 1104 16-17 0.43794991608769607 1104 18-19 0.2780977382814598 1104 20-21 0.41095929843987733 1104 22-23 0.49412407958931936 1104 24-25 0.5214589758834123 1104 26-27 0.3521144142863406 1104 28-29 0.509623418898606 1104 30-31 0.4670886750479184 1104 32-33 0.5830357768095098 1104 34-35 0.42986646804328643 1104 36-37 0.4232682230877103 1104 38-39 0.2941863327536183 1104 40-41 0.3181888193305298 1104 42-43 0.23612999278559954 1104 44-45 0.26632255888099365 1104 46-47 0.28705120809189566 1104 48-49 0.3449299526170577 1104 50-51 0.36412007714785943 1104 52-53 0.3775334269306114 1104 54-55 0.4747444285736151 1104 56-57 0.491995375550907 1104 58-59 0.4107955523078459 1104 60-61 0.3809603005852864 1104 62-63 0.4681791714476944 1104 64-65 0.47227652024516686 1104 66-67 0.4732748726667779 1104 68-69 0.2703563811173666 1104 70-71 0.2777265222457359 1104 72-73 0.3110752263359515 1104 74-75 0.2909799084115434 1104 76-77 0.4144022339496942 1104 78-79 0.4209972418066634 1104 80-81 0.43658371488558245 1104 82-83 0.4890010802921445 1104 84-85 0.28437379399398566 1104 86-87 0.2979873093817176 1104 88-89 0.3067211706727271 1104 90-91 0.4845801768775999 1104 92-93 0.5600766617568382 1104 94-95 0.45108591076677484 1104 96-97 0.5730166595283883 1104 98-99 0.7792424264908924 1104 100-101 1.1083715144779696 1105 1 0.29327219847264985 1105 2 0.22092068279905064 1105 3 0.19595270727564795 1105 4 0.07533192220060414 1105 5 0.2086235154608289 1105 6 0.21228611300680456 1105 7 0.26706738751689585 1105 8 0.2755657328783059 1105 9 0.3203127321657675 1105 10-11 0.37397434116128636 1105 12-13 0.3651022593938862 1105 14-15 0.3437646113577415 1105 16-17 0.40233522303046954 1105 18-19 0.3642794814221304 1105 20-21 0.4422299347561989 1105 22-23 0.5042428281313178 1105 24-25 0.4733044681941365 1105 26-27 0.36478264512597036 1105 28-29 0.38251481355469963 1105 30-31 0.47817934922740335 1105 32-33 0.47964996293114126 1105 34-35 0.26921240896958665 1105 36-37 0.31212719181023374 1105 38-39 0.4194518010185533 1105 40-41 0.42064308297292285 1105 42-43 0.4603679070820803 1105 44-45 0.43372783370289625 1105 46-47 0.4575785108356243 1105 48-49 0.3639826559745032 1105 50-51 0.35318742780373213 1105 52-53 0.3323904755784852 1105 54-55 0.475044776699292 1105 56-57 0.5624963221297676 1105 58-59 0.5780281101343903 1105 60-61 0.5371803554958348 1105 62-63 0.36669066497865543 1105 64-65 0.39146593919827666 1105 66-67 0.3809616761910988 1105 68-69 0.3313296301707922 1105 70-71 0.42131367479297666 1105 72-73 0.5300737593630274 1105 74-75 0.621231963070862 1105 76-77 0.6562188129867863 1105 78-79 0.6081596585884732 1105 80-81 0.671069354701114 1105 82-83 0.6536003768629293 1105 84-85 0.5098210637196132 1105 86-87 0.51713422200935 1105 88-89 0.5974421183282601 1105 90-91 0.7399296932700139 1105 92-93 0.521920112866713 1105 94-95 0.5550222881063043 1105 96-97 0.6526270817653632 1105 98-99 0.6711337000207784 1105 100-101 0.7039767641773942 1106 1 0.13876092027715714 1106 2 0.0951071664500347 1106 3 0.07371936228526366 1106 4 0.04905110387571909 1106 5 0.15360253277154357 1106 6 0.09067394304702248 1106 7 0.03292112068702835 1106 8 -0.06709207443066845 1106 9 0.04238608415037959 1106 10-11 0.11764019183447516 1106 12-13 0.196050414665784 1106 14-15 0.11192687748818742 1106 16-17 0.21276536816073843 1106 18-19 0.12429521843908731 1106 20-21 0.09874527834773517 1106 22-23 0.05918818571131368 1106 24-25 0.1500847182378564 1106 26-27 0.07192681368690756 1106 28-29 0.14079620016075012 1106 30-31 0.18042274842306227 1106 32-33 0.18582258926441142 1106 34-35 -0.03142953018060979 1106 36-37 -0.035420237810328103 1106 38-39 -0.07095714285274823 1106 40-41 -0.20652228684696894 1106 42-43 -0.13117366487105642 1106 44-45 -0.07674842962985196 1106 46-47 -0.10040452815720613 1106 48-49 -0.008136595642916689 1106 50-51 0.023294745516622584 1106 52-53 -0.0668574261525876 1106 54-55 -0.04995391188262488 1106 56-57 0.07279073548874493 1106 58-59 0.06224584924962073 1106 60-61 0.03463183302687156 1106 62-63 -0.04218326402991579 1106 64-65 -0.08513814368334494 1106 66-67 -0.049393718108603935 1106 68-69 0.10628744622254516 1106 70-71 0.03931835218412516 1106 72-73 0.06675652732945991 1106 74-75 0.06607787144645272 1106 76-77 0.06253066820622877 1106 78-79 0.16106051538162092 1106 80-81 0.2013786307983345 1106 82-83 0.161843950914335 1106 84-85 0.2261193676188995 1106 86-87 0.1843784954837311 1106 88-89 0.135755416107596 1106 90-91 0.12929627833732837 1106 92-93 0.07240446327762484 1106 94-95 0.1202150665640751 1106 96-97 0.08728873590070307 1106 98-99 0.1284243102673237 1106 100-101 0.05724591088136677 1107 1 -0.004822614831798688 1107 2 0.1295370329965735 1107 3 0.2313149476222769 1107 4 0.17997063763325372 1107 5 0.24304112728700034 1107 6 0.20370958694589092 1107 7 0.23953297946286511 1107 8 0.3162436989800028 1107 9 0.3514631718242356 1107 10-11 0.310590245328477 1107 12-13 0.20045494194772573 1107 14-15 0.37413927683499537 1107 16-17 0.15007321207087188 1107 18-19 0.2667199045137565 1107 20-21 0.2841735119194482 1107 22-23 0.25849445295609286 1107 24-25 0.38142938854586816 1107 26-27 0.3809464656943433 1107 28-29 0.41829042843937003 1107 30-31 0.33602948303650493 1107 32-33 0.39010379879197643 1107 34-35 0.3384387056434761 1107 36-37 0.22492268690390205 1107 38-39 0.3722339152023153 1107 40-41 0.46328119307869997 1107 42-43 0.09726656261922528 1107 44-45 0.38622640003396924 1107 46-47 0.4694715909936491 1107 48-49 0.4118926852850606 1107 50-51 0.3832108443960678 1107 52-53 0.42891542141765626 1107 54-55 0.591390195330618 1107 56-57 0.527290988881397 1107 58-59 0.6240815381960729 1107 60-61 0.6210499872022197 1107 62-63 0.6869850449964972 1107 64-65 0.4261901968763695 1107 66-67 0.4206631544630497 1107 68-69 0.6092949353829695 1107 70-71 0.6408330771123332 1107 72-73 0.535517718837994 1107 74-75 0.46290314313967684 1107 76-77 0.4730261916028198 1107 78-79 0.5779060584865299 1107 80-81 0.7019434148514883 1107 82-83 0.7723304647946172 1107 84-85 0.5960833295536929 1107 86-87 0.4219386058503076 1107 88-89 0.5702016124024318 1107 90-91 0.7995113805390304 1107 92-93 0.5317901268848111 1107 94-95 0.6257165024557381 1107 96-97 0.8454969683462217 1107 98-99 0.8600944493517311 1107 100-101 0.7382645493182132 1108 1 -0.03921580308900019 1108 2 0.07534201477715641 1108 3 0.03950729091469185 1108 4 0.041039611298010925 1108 5 0.04028946412837797 1108 6 0.04822219313550136 1108 7 0.035523190183816666 1108 8 0.0478511913768358 1108 9 0.05532772600668068 1108 10-11 0.016796986404649772 1108 12-13 -0.021483469700562807 1108 14-15 -0.04391788943317465 1108 16-17 0.051314430224827845 1108 18-19 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-1.3073325359206933 2316 52-53 -1.4218243675815074 2316 54-55 -1.4982267896015458 2316 56-57 -1.456824755718067 2316 58-59 -1.5885205553239388 2316 60-61 -1.6124586168072454 2316 62-63 -1.547010211318316 2316 64-65 -1.4994216634907858 2316 66-67 -1.545240928123576 2316 68-69 -1.682368990103079 2316 70-71 -1.3616136223825492 2316 72-73 -1.387913480826306 2316 74-75 -1.3950612437715755 2316 76-77 -1.560359244772524 2316 78-79 -1.6516740421086205 2316 80-81 -1.4450287141431097 2316 82-83 -1.5303077463562538 2316 84-85 -1.6552212663138448 2316 86-87 -1.6730130050982552 2316 88-89 -1.4856786528284367 2316 90-91 -1.6236405896896855 2316 92-93 -1.6983656628596293 2316 94-95 -1.6722649993155194 2316 96-97 -1.8959730959004553 2316 98-99 -1.5698988481525156 2316 100-101 -1.3322585668723939 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19514.0 3 4464.0 4 3592.0 5 2911.0 6 2919.0 7 3095.0 8 3697.0 9 4456.0 10 5072.0 11 6140.0 12 7929.0 13 11250.0 14 11924.0 15 10038.0 16 11786.0 17 14529.0 18 13403.0 19 10227.0 20 9890.0 21 10461.0 22 11047.0 23 12386.0 24 13946.0 25 16292.0 26 19245.0 27 23431.0 28 27753.0 29 33968.0 30 41241.0 31 51166.0 32 65041.0 33 85285.0 34 118653.0 35 176164.0 36 294471.0 37 510725.0 38 547909.0 39 105618.0 40 140.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.36510493219901 17.877750961029246 12.632800000344723 24.124344106427014 2 15.83099181261057 18.91378709513732 40.16800366315356 25.08721742909855 3 19.985173609196668 23.229964988697137 28.156875447979267 28.62798595412692 4 12.655391566719143 14.820159068932023 36.24984116796999 36.27460819637884 5 14.17433063818258 37.14849862830309 33.806158486235866 14.871012247278466 6 34.46440647303834 34.63557931281039 16.75310019253714 14.146914021614124 7 30.755758860116124 31.23279233645182 19.597827397873914 18.41362140555814 8 27.332796625026546 35.33108570576927 18.783627726023763 18.552489943180422 9 27.10131617376561 15.874854507531147 19.292710601156358 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23.367422839789395 26.203476892151134 27.385356806259832 23.043743461799636 36-37 23.398332125779188 26.168689941849184 27.296139511770246 23.136838420601386 38-39 23.140958937004722 26.253967086433267 27.562115865966025 23.042958110595986 40-41 23.320778086315503 26.25542552636721 27.490278465093464 22.933517922223825 42-43 23.309096746862462 26.178499149058254 27.345217525635352 23.167186578443935 44-45 23.25702826800911 26.326535072581308 27.28913573028485 23.12730092912473 46-47 23.225928477940208 26.53362031952245 26.938606711771918 23.30184449076543 48-49 23.5540319290648 26.428405245690183 26.91181037775765 23.10575244748737 50-51 23.310077749247906 26.566516071374075 26.831444885824123 23.291961293553896 52-53 23.49639994745302 26.608942898874982 26.779266103929185 23.11539104974282 54-55 23.37462610054598 26.381510199992253 26.937596037763978 23.306267661697785 56-57 23.320303582730624 26.75786410182896 26.824660957811087 23.09717135762933 58-59 23.483528422286206 26.548203239290917 26.80517235820664 23.163095980216234 60-61 23.518492634060618 26.42200516782 27.136853757444808 22.92264844067458 62-63 23.38754695099787 26.542372734668447 26.932801654752218 23.137278659581465 64-65 23.266121109246228 26.41830358418068 26.985767841484815 23.32980746508828 66-67 23.385332709978304 26.69530897936287 26.73955103293837 23.17980727772046 68-69 23.09408190293178 26.542215239727508 26.939135440200126 23.424567417140583 70-71 23.015540061770796 26.568193988435556 27.01151576453534 23.404750185258308 72-73 23.29600127404605 26.54064256668348 26.760592942070506 23.40276321719997 74-75 23.15953353201238 26.68857892687779 26.69457349108487 23.457314050024962 76-77 23.18493711645125 26.720774886134933 26.726876828824714 23.367411168589104 78-79 23.268646753283285 26.596992544652565 26.672119424041686 23.462241278022468 80-81 23.042098186184464 26.938481136409326 26.756391999503176 23.263028677903034 82-83 23.253357222301563 26.995702582345533 26.544430451510152 23.206509743842748 84-85 23.305082361881617 26.697449319843148 26.43588548489495 23.56158283338029 86-87 23.141895949159302 26.549459604439146 26.879299215789715 23.429345230611833 88-89 23.352942869614655 26.623521791343368 26.616423316671394 23.407112022370583 90-91 23.25940084813823 26.912759523154957 26.660764190296415 23.1670754384104 92-93 23.289647095338825 26.725190107752077 26.720125329454085 23.26503746745501 94-95 23.197820262067957 26.75662067050916 26.665567450031624 23.37999161739125 96-97 23.237941665183012 26.73336230698325 26.590974628730947 23.4377213991028 98-99 23.16459118803036 26.621609636969694 26.502813090116224 23.71098608488372 100-101 23.27655505704508 26.736900399337177 26.358698238676432 23.62784630494131 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 119.0 1 89.0 2 139.5 3 249.5 4 212.5 5 194.5 6 421.5 7 772.0 8 1171.5 9 1392.5 10 1332.0 11 1365.5 12 1562.5 13 1797.0 14 2061.5 15 2315.0 16 2497.5 17 2641.0 18 2627.5 19 2643.5 20 2773.0 21 2934.5 22 3305.0 23 3987.5 24 4800.5 25 5916.5 26 7820.5 27 10105.0 28 12530.5 29 15325.0 30 18875.0 31 22902.0 32 26904.5 33 31350.0 34 36581.5 35 40945.0 36 45658.0 37 50991.5 38 55464.0 39 59189.5 40 62615.5 41 68856.5 42 74509.0 43 78474.0 44 84070.0 45 89136.5 46 91203.5 47 91819.5 48 95608.5 49 95805.0 50 92642.5 51 90058.0 52 87697.0 53 87024.5 54 85161.5 55 80841.0 56 74404.0 57 66985.0 58 61189.0 59 55129.5 60 47347.5 61 39491.0 62 32028.5 63 25528.0 64 20123.0 65 15847.5 66 11781.5 67 9412.5 68 6848.5 69 4252.5 70 3057.5 71 2252.0 72 1715.5 73 1393.5 74 1079.5 75 820.5 76 533.5 77 297.5 78 190.0 79 112.0 80 67.0 81 52.0 82 43.5 83 32.0 84 26.5 85 24.5 86 19.5 87 18.5 88 20.5 89 18.5 90 19.0 91 23.0 92 18.0 93 16.5 94 20.5 95 24.5 96 24.5 97 22.0 98 19.5 99 13.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04690370914015035 2 0.012102793634878097 3 0.010423046475588966 4 0.00615907291739348 5 0.008657158436336292 6 0.006374425117302344 7 0.005943720717484617 8 0.009432426356008198 9 0.015160794873583952 10-11 0.0025196207389336965 12-13 0.009389355916026425 14-15 0.009174003716117561 16-17 0.019144810571897916 18-19 0.011930511874951007 20-21 0.003811733938386874 22-23 0.01063839867549783 24-25 0.022073600490658454 26-27 0.007515791776819317 28-29 0.018907923151998168 30-31 0.014751625693757112 32-33 0.01313648419444064 34-35 0.26102840150953277 36-37 0.4579464531061971 38-39 0.4553837619272816 40-41 0.4762513900984504 42-43 0.4655483857629799 44-45 0.48077378629653655 46-47 0.4879880849934835 48-49 0.49533159501037566 50-51 0.504936303126311 52-53 0.4937810591710319 54-55 0.4808168567365183 56-57 0.4755191926187603 58-59 0.4735379523795987 60-61 0.4711475429606104 62-63 0.4676588373220868 64-65 0.4839394636351968 66-67 0.45777417134627 68-69 0.4840256045151604 70-71 0.4658283436228614 72-73 0.4750454177789608 74-75 0.4889140994530915 76-77 0.4754330517387968 78-79 0.4746577838191248 80-81 0.47793113725773956 82-83 0.47756503851789445 84-85 0.4764021366383866 86-87 0.4784910529775026 88-89 0.4919721006917974 90-91 0.5174051955010341 92-93 0.5042041056466208 94-95 0.475562263058742 96-97 0.45148588710893117 98-99 0.4420319255329321 100-101 0.44999995692956 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2321778.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.387601474660514 #Duplication Level Percentage of deduplicated Percentage of total 1 71.52565396324766 36.040061471145954 2 14.536549737645426 14.649237499941172 3 5.689142318091749 8.599867075699 4 2.7361185250011757 5.514657993007808 5 1.5105630736901328 3.8056825079718326 6 0.9461911776814788 2.860578238791243 7 0.6304967677423838 2.223845390484538 8 0.4300420130374686 1.733502845623418 9 0.3198355384443286 1.4504171083709663 >10 1.5182913816623342 13.367506937225807 >50 0.10075388895884134 3.539776083702543 >100 0.05362363981019662 4.799914675049169 >500 0.0021390429585713346 0.7554171838840112 >1k 5.989320283997821E-4 0.6595349891025497 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4548 0.19588436103710172 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3058 0.13170940546426058 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 4.307043998177259E-5 0.0 0.0 0.0 0.0 12-13 4.307043998177259E-5 0.0 0.0 0.0 0.0 14-15 4.307043998177259E-5 0.0 0.0 0.0 0.0 16-17 4.307043998177259E-5 0.0 0.0 2.1535219990886296E-5 0.0 18-19 4.307043998177259E-5 0.0 0.0 4.307043998177259E-5 0.0 20-21 4.307043998177259E-5 0.0 0.0 1.9381697991797665E-4 0.0 22-23 4.307043998177259E-5 0.0 0.0 6.029861597448162E-4 0.0 24-25 4.307043998177259E-5 0.0 0.0 0.001076760999544315 0.0 26-27 4.307043998177259E-5 0.0 0.0 0.0014428597393893816 0.0 28-29 4.307043998177259E-5 0.0 0.0 0.001981240239161539 0.0 30-31 4.307043998177259E-5 0.0 0.0 0.0035317760785053528 0.0 32-33 4.307043998177259E-5 0.0 0.0 0.006202143357375253 0.0 34-35 4.307043998177259E-5 0.0 0.0 0.010186159055689217 0.0 36-37 4.307043998177259E-5 0.0 0.0 0.017809626932462966 0.0 38-39 4.307043998177259E-5 0.0 0.0 0.030644618047031197 0.0 40-41 4.307043998177259E-5 0.0 0.0 0.043544214821572086 0.0 42-43 4.307043998177259E-5 0.0 0.0 0.05713293863582134 0.0 44-45 4.307043998177259E-5 0.0 0.0 0.07339202972894049 0.0 46-47 4.307043998177259E-5 0.0 0.0 0.09387202394027336 0.0 48-49 4.307043998177259E-5 0.0 0.0 0.11790532945010246 0.0 50-51 4.307043998177259E-5 0.0 0.0 0.14428597393893816 0.0 52-53 4.307043998177259E-5 0.0 0.0 0.17185105552727264 0.0 54-55 4.307043998177259E-5 0.0 0.0 0.19894236227580758 0.0 56-57 4.307043998177259E-5 0.0 0.0 0.23202046018180894 0.0 58-59 4.307043998177259E-5 0.0 0.0 0.2710638140252858 0.0 60-61 4.307043998177259E-5 0.0 0.0 0.3167615508459465 0.0 62-63 4.307043998177259E-5 0.0 0.0 0.36403135872594194 0.0 64-65 4.307043998177259E-5 0.0 0.0 0.41319626596513537 0.0 66-67 4.307043998177259E-5 0.0 0.0 0.47106140208064684 0.0 68-69 4.307043998177259E-5 0.0 0.0 0.5335996809341806 0.0 70-71 4.307043998177259E-5 0.0 0.0 0.6050750760839323 0.0 72-73 4.307043998177259E-5 0.0 0.0 0.6809651913318155 0.0 74-75 4.307043998177259E-5 0.0 0.0 0.7572429405395349 0.0 76-77 4.307043998177259E-5 0.0 0.0 0.8446328632625514 0.0 78-79 4.307043998177259E-5 0.0 0.0 0.9467744116793251 0.0 80-81 4.307043998177259E-5 0.0 0.0 1.061772486430658 0.0 82-83 4.307043998177259E-5 0.0 0.0 1.1830588454193296 0.0 84-85 6.460565997265889E-5 0.0 0.0 1.3051850779876455 0.0 86-87 8.614087996354519E-5 0.0 0.0 1.4346548205728542 0.0 88-89 8.614087996354519E-5 2.1535219990886296E-5 0.0 1.5863058397486753 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5890 0.0 37.704258 1 GGTATCA 3920 0.0 33.58084 1 ATCAACG 7010 0.0 30.661009 3 TCAACGC 7125 0.0 30.16613 4 GTACATG 9170 0.0 29.794718 1 CAACGCA 7300 0.0 29.442968 5 TATCAAC 7485 0.0 28.967628 2 AACGCAG 7465 0.0 28.917778 6 TACATGG 9325 0.0 28.683945 2 ACATGGG 9270 0.0 27.986557 3 CATGGGG 4790 0.0 24.610046 4 ACGCAGA 8850 0.0 24.392754 7 CGCAGAG 9075 0.0 23.684662 8 GTACCTG 2000 0.0 23.202185 1 TACCTGG 1940 0.0 21.962412 2 CATGGGA 4905 0.0 21.42706 4 AGTACAT 6310 0.0 21.382317 2 GCAGAGT 10235 0.0 20.352297 9 GAGTACA 7770 0.0 19.98875 1 ATACCGT 195 1.0638929E-5 19.421612 6 >>END_MODULE