FastQCFastQC Report
Thu 26 May 2016
SRR936309_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936309_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2321778
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45520.1960566427970288No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33560.1445443965788288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23240.10009570251763951No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA66000.037.2627451
GGTATCA44000.035.4608731
TCAACGC78700.030.6792244
ATCAACG78650.030.6384143
CAACGCA80700.029.7425585
AACGCAG81750.029.4765916
TATCAAC83050.029.2531032
GTACATG90900.027.8404351
TACATGG92550.026.9168682
ACATGGG89950.026.5785123
ACGCAGA96750.024.857557
TACCTGG20050.024.1394542
CGCAGAG99600.024.0033868
CATGGGG49150.023.9347844
GTACCTG21650.022.850921
GCAGAGT110600.021.1442939
CTCGCGC4900.019.84600660-61
AGTACAT63450.019.8178222
GAGTACT51400.019.79588312-13
TCGCGGA4950.019.792682-83
Produced by FastQC (version 0.11.5)