##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936309_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2321778 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48995726550945 34.0 31.0 34.0 31.0 34.0 2 32.623540665817316 34.0 31.0 34.0 31.0 34.0 3 32.70987923910038 34.0 31.0 34.0 31.0 34.0 4 36.19350428852371 37.0 35.0 37.0 35.0 37.0 5 36.155349047152654 37.0 35.0 37.0 35.0 37.0 6 36.189849761691256 37.0 37.0 37.0 35.0 37.0 7 36.138726872250494 37.0 36.0 37.0 35.0 37.0 8 36.120709215092916 37.0 36.0 37.0 35.0 37.0 9 37.89064501429508 39.0 38.0 39.0 35.0 39.0 10-11 37.82698647329762 39.0 38.0 39.0 35.0 39.0 12-13 37.76949648071435 39.0 38.0 39.0 35.0 39.0 14-15 39.13163166332009 40.5 38.5 41.0 36.0 41.0 16-17 39.05800166941025 40.0 38.0 41.0 36.0 41.0 18-19 39.07315751118324 40.0 38.5 41.0 36.0 41.0 20-21 39.027012487843365 40.0 38.5 41.0 35.0 41.0 22-23 38.91152685571144 40.0 38.0 41.0 35.0 41.0 24-25 39.06703827842283 40.0 39.0 41.0 35.0 41.0 26-27 38.99899473593082 40.0 39.0 41.0 35.0 41.0 28-29 38.90772352050885 40.0 38.0 41.0 35.0 41.0 30-31 38.80819074864178 40.0 38.0 41.0 35.0 41.0 32-33 38.674235219732466 40.0 38.0 41.0 35.0 41.0 34-35 38.547602957733254 40.0 38.0 41.0 34.0 41.0 36-37 38.413482899743215 40.0 38.0 41.0 34.0 41.0 38-39 38.28981819105874 40.0 38.0 41.0 34.0 41.0 40-41 38.15870875682344 40.0 38.0 41.0 33.0 41.0 42-43 38.194695616893604 40.0 38.0 41.0 34.0 41.0 44-45 38.076289808930916 40.0 38.0 41.0 33.0 41.0 46-47 37.89213848180145 40.0 38.0 41.0 33.0 41.0 48-49 37.68780348508772 40.0 37.0 41.0 33.0 41.0 50-51 37.49867752214036 40.0 37.0 41.0 33.0 41.0 52-53 37.29209209493759 40.0 37.0 41.0 32.0 41.0 54-55 37.10105466586383 40.0 36.0 41.0 32.0 41.0 56-57 36.86435223350381 39.0 36.0 41.0 31.0 41.0 58-59 36.610304688906524 39.0 35.0 41.0 31.0 41.0 60-61 36.336478121508605 39.0 35.0 41.0 31.0 41.0 62-63 36.04656496012969 38.0 35.0 40.0 30.0 41.0 64-65 35.67344896885059 37.5 35.0 40.0 29.5 41.0 66-67 35.326794594487495 37.0 35.0 40.0 29.0 41.0 68-69 34.972993111313826 36.5 34.0 39.0 29.0 41.0 70-71 34.59732024336522 36.0 34.0 39.0 29.0 41.0 72-73 34.18163945906973 35.5 34.0 38.5 28.5 40.0 74-75 33.74536497460136 35.0 34.0 37.5 28.0 39.5 76-77 33.00819673543293 35.0 32.5 37.0 26.5 39.0 78-79 33.05285819746763 35.0 33.0 36.5 27.0 39.0 80-81 32.87283581806702 35.0 33.0 36.0 27.0 38.0 82-83 32.58415942437219 35.0 33.0 36.0 27.0 37.0 84-85 32.29105495874282 35.0 33.0 35.0 26.0 37.0 86-87 31.88739556495065 35.0 32.5 35.0 25.5 36.0 88-89 31.40153322152247 35.0 32.0 35.0 24.5 36.0 90-91 31.188308270644306 35.0 32.0 35.0 24.0 36.0 92-93 30.905025588148394 34.0 32.0 35.0 22.5 35.0 94-95 30.749685585788136 34.0 32.0 35.0 20.0 35.0 96-97 30.50737581284688 34.0 31.0 35.0 19.5 35.0 98-99 30.0640330384731 34.0 31.0 35.0 9.0 35.0 100-101 26.67624682463181 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13779343096530283 1101 2 0.11704666876186565 1101 3 0.18521185428200226 1101 4 0.1050809967979589 1101 5 0.10666602656854707 1101 6 0.13737858232389755 1101 7 0.11452332179212732 1101 8 0.1273646033996414 1101 9 0.12237767147360046 1101 10-11 0.13541803237106365 1101 12-13 0.2534390971732421 1101 14-15 0.2679566029758007 1101 16-17 0.294084022130896 1101 18-19 0.3246106473905357 1101 20-21 0.22878093648054687 1101 22-23 0.30465896076388077 1101 24-25 0.21887822087686715 1101 26-27 0.23704420723667852 1101 28-29 0.2656081434108586 1101 30-31 0.26539079789708353 1101 32-33 0.24095029595903839 1101 34-35 0.33261027247082353 1101 36-37 0.30764968173255625 1101 38-39 0.36460436470412105 1101 40-41 0.4065950644851881 1101 42-43 0.320601148119799 1101 44-45 0.3005602299993697 1101 46-47 0.3585695794894761 1101 48-49 0.3462893406106602 1101 50-51 0.36346181039256464 1101 52-53 0.28441370891031426 1101 54-55 0.3106385178589832 1101 56-57 0.3167373052239455 1101 58-59 0.26301650811074495 1101 60-61 0.35059513653451546 1101 62-63 0.46453225492908246 1101 64-65 0.39590814673851327 1101 66-67 0.3360393292368329 1101 68-69 0.49237690870383943 1101 70-71 0.43606023696615637 1101 72-73 0.44113624389535033 1101 74-75 0.42962220323014577 1101 76-77 0.26745926830275124 1101 78-79 0.2605468455068092 1101 80-81 0.32684833973922167 1101 82-83 0.2994191113054896 1101 84-85 0.5142673163505833 1101 86-87 0.32945553931304516 1101 88-89 0.37769108869906276 1101 90-91 0.49934198614619874 1101 92-93 0.6710547963057287 1101 94-95 0.7039924849966717 1101 96-97 0.6496350327887939 1101 98-99 0.8027209278889771 1101 100-101 1.0335452061245824 1102 1 0.02816264572109617 1102 2 0.10509434223768466 1102 3 0.1591521258687436 1102 4 0.0402033363269112 1102 5 0.10685377748238523 1102 6 0.11652641955886622 1102 7 0.143666299157438 1102 8 0.106590444618476 1102 9 0.07292486819005006 1102 10-11 0.17627440292197605 1102 12-13 0.2371584107873943 1102 14-15 0.25238041313821213 1102 16-17 0.2537357847871675 1102 18-19 0.3824287012526497 1102 20-21 0.25758261565778184 1102 22-23 0.2612824709677568 1102 24-25 0.2398226596480484 1102 26-27 0.30172956451091437 1102 28-29 0.1059843096257822 1102 30-31 0.202830618561741 1102 32-33 0.13111921026415274 1102 34-35 0.09738950520198131 1102 36-37 0.07319111427448632 1102 38-39 0.04531486534450835 1102 40-41 0.019474211215111836 1102 42-43 0.024851750066943623 1102 44-45 -0.017044924661369976 1102 46-47 0.08083344211388521 1102 48-49 0.05178763719558077 1102 50-51 0.042919695008187375 1102 52-53 0.028548707476957702 1102 54-55 0.023150036980418065 1102 56-57 0.0412463045952407 1102 58-59 0.038318773548219554 1102 60-61 -0.0034612551783652634 1102 62-63 0.10250456966655008 1102 64-65 0.19737287454274366 1102 66-67 0.20848276096919705 1102 68-69 0.09637405922077136 1102 70-71 0.10487180813125718 1102 72-73 0.00819457094366527 1102 74-75 0.12233510550739624 1102 76-77 0.08169499535770797 1102 78-79 0.01467791926732076 1102 80-81 -0.0010069567189887607 1102 82-83 0.0997105804502425 1102 84-85 0.1274672144778748 1102 86-87 -0.025323435045301323 1102 88-89 -0.033483166541291354 1102 90-91 0.1615579144612731 1102 92-93 0.07614474080478217 1102 94-95 -0.014071958105326132 1102 96-97 -0.09877751231879373 1102 98-99 -0.015861860298215902 1102 100-101 0.5598644146671248 1103 1 0.031207821837668348 1103 2 0.10533168373041235 1103 3 0.11431130124832833 1103 4 -0.013981161365016703 1103 5 0.07545006030962043 1103 6 0.12261206263540458 1103 7 0.19869773866420104 1103 8 0.1722440932141467 1103 9 0.11764560931711543 1103 10-11 0.2517754866166442 1103 12-13 0.24622895277600065 1103 14-15 0.27751730243419104 1103 16-17 0.3298848484302397 1103 18-19 0.2652504043426802 1103 20-21 0.254668354094747 1103 22-23 0.25787304158803437 1103 24-25 0.19884157750740883 1103 26-27 0.25274444662306905 1103 28-29 0.29376357732838443 1103 30-31 0.2983530347358112 1103 32-33 0.23665600113875485 1103 34-35 0.2898180054886481 1103 36-37 0.2808284244513217 1103 38-39 0.35125264090375197 1103 40-41 0.4285582920289528 1103 42-43 0.4207133432452608 1103 44-45 0.33569774700799115 1103 46-47 0.29403002938809664 1103 48-49 0.30324105951627445 1103 50-51 0.3819435339362016 1103 52-53 0.2938622372370645 1103 54-55 0.1761276955642117 1103 56-57 0.23762378587928623 1103 58-59 0.3001462324049058 1103 60-61 0.2193008064433073 1103 62-63 0.27513459830998954 1103 64-65 0.1399409424582032 1103 66-67 0.11987680082514629 1103 68-69 0.02685195620561842 1103 70-71 -0.059402410766821845 1103 72-73 0.07291356968278961 1103 74-75 0.06430770057054502 1103 76-77 0.10398202080716601 1103 78-79 -0.013907720865994122 1103 80-81 0.08969481357070919 1103 82-83 0.21698376686573084 1103 84-85 0.2912269580172051 1103 86-87 0.14626928412767626 1103 88-89 0.26548564589182533 1103 90-91 0.3312821580235621 1103 92-93 0.384824540524356 1103 94-95 0.3571775812802933 1103 96-97 0.2646987399931078 1103 98-99 0.16615575430205354 1103 100-101 0.6143885602789574 1104 1 0.006658303453029646 1104 2 0.08285934959695851 1104 3 0.007481774675717645 1104 4 0.012472633178546744 1104 5 0.048249284510347934 1104 6 0.04446881194039776 1104 7 0.10565488787634081 1104 8 0.07414276587007862 1104 9 0.04494553281215019 1104 10-11 0.0639946682078758 1104 12-13 0.15773683255913795 1104 14-15 0.1643470119783217 1104 16-17 0.1167740237203958 1104 18-19 0.13156523201209325 1104 20-21 0.1567663513570352 1104 22-23 0.14322731448778114 1104 24-25 0.1356217132036619 1104 26-27 0.23297688907565828 1104 28-29 0.19281852939556643 1104 30-31 0.1257176844082295 1104 32-33 0.1289466102395238 1104 34-35 0.19503864055995024 1104 36-37 0.22215700357450174 1104 38-39 0.2751067531316238 1104 40-41 0.35906948016599216 1104 42-43 0.2833407228757281 1104 44-45 0.29478211174799895 1104 46-47 0.3298556255036118 1104 48-49 0.2553400288147998 1104 50-51 0.3105951595810268 1104 52-53 0.3119208407297549 1104 54-55 0.27172597242986996 1104 56-57 0.23533835715983997 1104 58-59 0.32605545794361035 1104 60-61 0.26777305137813556 1104 62-63 0.21238828718749403 1104 64-65 0.12304180665559272 1104 66-67 0.11583324445333432 1104 68-69 0.20897220737978017 1104 70-71 0.14958983924547198 1104 72-73 0.054569759323626954 1104 74-75 0.04452905037437205 1104 76-77 0.059671691950157424 1104 78-79 0.13300457745078376 1104 80-81 0.11564143391507997 1104 82-83 -0.013994684806341695 1104 84-85 0.20593237957788801 1104 86-87 0.08553428750693115 1104 88-89 0.15675801844929538 1104 90-91 0.35866521920581107 1104 92-93 0.31303554786265764 1104 94-95 0.23957103181451345 1104 96-97 0.17480883167328187 1104 98-99 0.19882737873469836 1104 100-101 0.5830470244845785 1105 1 -0.016588282582461034 1105 2 0.1066673780212426 1105 3 0.11765233831632571 1105 4 0.09405741254941091 1105 5 0.03307396554468056 1105 6 0.08460016657998182 1105 7 0.15077036152466405 1105 8 0.0792334310772489 1105 9 0.044538638488035076 1105 10-11 0.08395750040770622 1105 12-13 0.17056925669824352 1105 14-15 0.0982843314105537 1105 16-17 0.15306657490743447 1105 18-19 0.16692522357812578 1105 20-21 0.18448552249491001 1105 22-23 0.04517363314839429 1105 24-25 0.11306442499507341 1105 26-27 0.11793862216734396 1105 28-29 0.002341179995809739 1105 30-31 0.017572171461189612 1105 32-33 0.04952617669744086 1105 34-35 -0.028648485967586623 1105 36-37 -0.017255556764290247 1105 38-39 -8.708923543991887E-4 1105 40-41 0.08056478316095195 1105 42-43 0.240666278710421 1105 44-45 0.1554552369835278 1105 46-47 0.06307605725123722 1105 48-49 0.1012639077244799 1105 50-51 0.029824841781753264 1105 52-53 0.1395003802707322 1105 54-55 0.11506178810270029 1105 56-57 0.06017977155502052 1105 58-59 0.09945737712102698 1105 60-61 0.13007521191072868 1105 62-63 0.11553083927176999 1105 64-65 0.04436697219474439 1105 66-67 0.03488964888924073 1105 68-69 0.12278822116125099 1105 70-71 0.07575321157845138 1105 72-73 0.01724853262920334 1105 74-75 0.22617405638582255 1105 76-77 0.13132367393374977 1105 78-79 0.07529175422325807 1105 80-81 0.05951300873518761 1105 82-83 0.14303556982785892 1105 84-85 0.21786712395672936 1105 86-87 0.2631435478295714 1105 88-89 0.25742881088384095 1105 90-91 0.272663034447973 1105 92-93 0.41731892934739534 1105 94-95 0.2899304850418538 1105 96-97 0.3725703521211763 1105 98-99 0.3869810116236039 1105 100-101 0.6095964305175592 1106 1 -0.07897637490629705 1106 2 -0.024843375607012774 1106 3 -0.04457794669853854 1106 4 -0.04534720364223688 1106 5 -0.03461356724077547 1106 6 -0.03801217823554737 1106 7 0.017752351383030884 1106 8 -0.006296818966461615 1106 9 -0.133710181235692 1106 10-11 0.08266203791426818 1106 12-13 0.036192180286285236 1106 14-15 0.039012605584360927 1106 16-17 -0.050489116647035814 1106 18-19 0.04958543515357405 1106 20-21 0.06722092204027774 1106 22-23 0.06403488511552524 1106 24-25 0.11988379901151092 1106 26-27 -0.014233523312640273 1106 28-29 -0.035348757056127056 1106 30-31 0.09685438863202478 1106 32-33 -0.06811283361905396 1106 34-35 0.0658090240866187 1106 36-37 -0.06549410371305697 1106 38-39 0.0968273503453645 1106 40-41 0.07321056913751534 1106 42-43 0.0638746630433431 1106 44-45 0.06603204236908056 1106 46-47 0.09876498888264251 1106 48-49 0.03357724921774974 1106 50-51 -0.02659804683514011 1106 52-53 -0.10552934520574553 1106 54-55 -0.03291075954549427 1106 56-57 -0.022442627465785847 1106 58-59 0.019078158726209438 1106 60-61 -0.06650679543320592 1106 62-63 -0.1507998129401642 1106 64-65 0.012328591358929941 1106 66-67 -0.047924872880294345 1106 68-69 -0.01575610476985645 1106 70-71 -0.1713592643788857 1106 72-73 -0.1901740937040728 1106 74-75 -0.01342399397263705 1106 76-77 -0.04623883699473197 1106 78-79 -0.1902612270671682 1106 80-81 -0.06477816979252538 1106 82-83 0.04752499105534724 1106 84-85 0.068488298987333 1106 86-87 0.007512974302734676 1106 88-89 -0.003840860387359868 1106 90-91 0.11129107256652304 1106 92-93 0.18886115700957262 1106 94-95 0.22210197481279081 1106 96-97 0.0897208592028349 1106 98-99 -0.03133469042220938 1106 100-101 0.30467271133588625 1107 1 -0.04569972644635811 1107 2 -0.020228250881523024 1107 3 0.059939199537168975 1107 4 0.07528909344656398 1107 5 0.006423653323444967 1107 6 0.09813400116644999 1107 7 0.021963174064488555 1107 8 -0.012774661187307856 1107 9 -0.03400411670503445 1107 10-11 0.0815465471771688 1107 12-13 0.04287555209776173 1107 14-15 0.10578719874839493 1107 16-17 0.15847309969397116 1107 18-19 0.12793500438607452 1107 20-21 0.1075317503526847 1107 22-23 0.1269421434070992 1107 24-25 0.11686842652432006 1107 26-27 0.11358504755284571 1107 28-29 0.10753822982377415 1107 30-31 0.018556624118254206 1107 32-33 -0.07204098272654846 1107 34-35 -0.021599613787138594 1107 36-37 0.08870770945210182 1107 38-39 0.107279515444759 1107 40-41 -0.024968382380023968 1107 42-43 -0.10443774193441158 1107 44-45 -0.028842105950076302 1107 46-47 0.08257714046775533 1107 48-49 -0.019291718629538934 1107 50-51 0.0751909052454991 1107 52-53 0.04481132712940905 1107 54-55 0.1630450938246284 1107 56-57 0.12432689784532869 1107 58-59 -0.030734893810539177 1107 60-61 0.07964670420077624 1107 62-63 0.14977003896591157 1107 64-65 0.27310566213994747 1107 66-67 0.01063754617482715 1107 68-69 0.12673558958230302 1107 70-71 0.2833667900160748 1107 72-73 0.17324445463761862 1107 74-75 0.1292349395258725 1107 76-77 -0.044125542327236644 1107 78-79 -0.008350083421696297 1107 80-81 0.12184495087868896 1107 82-83 0.29832740110095557 1107 84-85 0.2751390113022154 1107 86-87 0.321346523489062 1107 88-89 0.3751258607118011 1107 90-91 0.07401307267914348 1107 92-93 0.26640775310169573 1107 94-95 0.25999149561914336 1107 96-97 0.1563467953478863 1107 98-99 0.13468873432017148 1107 100-101 0.2035853753372372 1108 1 -0.10506077872882713 1108 2 -0.048075454889144 1108 3 0.005943175076978946 1108 4 -0.02876166513585332 1108 5 -0.15037172343156868 1108 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-0.7240620983916628 2316 74-75 -0.6854297584372446 2316 76-77 -0.7492107827390839 2316 78-79 -0.9411687343321375 2316 80-81 -0.8628313960715133 2316 82-83 -1.0179871990452938 2316 84-85 -0.8341271684648248 2316 86-87 -0.8900281806489225 2316 88-89 -0.8210279125130064 2316 90-91 -0.9709656203094532 2316 92-93 -0.8900838984575614 2316 94-95 -0.7283630622840711 2316 96-97 -0.579719639328335 2316 98-99 -0.682073935499055 2316 100-101 -0.6586127959449151 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 13.0 5 33.0 6 66.0 7 141.0 8 330.0 9 661.0 10 1059.0 11 1710.0 12 2736.0 13 4082.0 14 4971.0 15 6489.0 16 8577.0 17 11879.0 18 10988.0 19 7288.0 20 6736.0 21 7058.0 22 7741.0 23 8769.0 24 10500.0 25 13073.0 26 16173.0 27 20304.0 28 25772.0 29 32196.0 30 39796.0 31 49505.0 32 63096.0 33 81038.0 34 112417.0 35 166764.0 36 284915.0 37 543302.0 38 650058.0 39 121384.0 40 150.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.55073560275896 18.33146776804114 13.051313409786964 24.066483219412934 2 16.018189917140756 19.233347377272768 39.72565207389353 25.02281063169295 3 19.913101080292776 23.30369225653788 28.521676060329625 28.26153060283972 4 12.851314811321323 15.234574537272728 36.06042438165923 35.85368626974672 5 14.113709407187077 37.1625538703528 33.891052460657306 14.83268426180281 6 34.457342605537654 34.8695697866032 16.57445285466569 14.098634753193458 7 30.567780382103717 31.249499306135213 19.65106052344367 18.531659788317402 8 27.203677526447407 35.54086566415911 18.691881825049595 18.563574984343894 9 26.9125644226106 15.822830606543778 19.336689382016715 37.92791558882891 10-11 25.98670106292003 26.061385640102603 26.731500976950933 21.220412320026426 12-13 26.20724958119336 22.939276428750325 27.560459656844515 23.293014333211804 14-15 22.878608549137773 24.532448838777867 25.34811683115268 27.240825780931683 16-17 23.199234030016857 28.022079782510005 25.78892668585324 22.98975950161989 18-19 23.010382560262006 26.652612782100615 27.70269164407622 22.634313013561158 20-21 23.66020041998049 25.868855911717525 27.742444410514455 22.728499257787522 22-23 23.34989033186858 25.890594598319606 27.531901743384108 23.227613326427704 24-25 23.07333985580078 26.02916761872725 27.81179113660851 23.085701388863463 26-27 23.184842398694414 26.34925160861829 27.558595743746107 22.90731024894119 28-29 22.87775817300455 26.309426206118886 27.796711351335983 23.01610426954058 30-31 23.007214190542584 26.189154831272276 27.780954269926887 23.022676708258256 32-33 22.91938194576649 26.200846090299596 27.73986556622668 23.139906397707236 34-35 23.28338653344457 26.19316916601665 27.512806144709756 23.010638155829017 36-37 23.302688675689936 26.198511914375857 27.428626804922956 23.07017260501125 38-39 23.02084697231774 26.23446447891614 27.724035393595248 23.02065315517087 40-41 23.1863661726509 26.184917483739127 27.648444312264715 22.98027203134526 42-43 23.188150173494456 26.167684891337682 27.475698715761983 23.168466219405882 44-45 23.14066406493125 26.35907868329302 27.35619044255274 23.144066809222988 46-47 23.059173719618638 26.583190730703414 27.019043239558414 23.338592310119527 48-49 23.434279341391267 26.469078015834146 26.963577332852594 23.133065309921992 50-51 23.215813281943888 26.535954666099247 26.925015226449826 23.32321682550704 52-53 23.390740172403582 26.536712486606685 26.89379573244601 23.178751608543724 54-55 23.323977681677704 26.352185262145962 26.994949326365088 23.32888772981125 56-57 23.185097701736094 26.770689177670416 26.93574000876079 23.108473111832705 58-59 23.386600239346816 26.521604205130174 26.89096152425632 23.200834031266695 60-61 23.402094425525398 26.382344751305542 27.241637663565754 22.973923159603306 62-63 23.30961444203537 26.466203917859506 27.009386771689627 23.2147948684155 64-65 23.19844255746192 26.3857512958947 27.040708429614668 23.375097717028716 66-67 23.20460870935981 26.65857803803809 26.886377595101685 23.250435657500415 68-69 22.96769975424007 26.512870739579753 27.086202901397115 23.43322660478306 70-71 22.963607745206623 26.485358270613624 27.128078710588017 23.42295527359174 72-73 23.23028779486769 26.512717506543044 26.814796916365115 23.442197782224156 74-75 23.029376624293967 26.642900397884723 26.765715757492746 23.56200722032856 76-77 23.15042066468753 26.68569563135736 26.782444302088017 23.381439401867095 78-79 23.16279170035031 26.60757747237941 26.79023443815683 23.439396389113444 80-81 22.887745402280782 26.935900478475496 26.827598504403362 23.348755614840364 82-83 23.154862351180864 26.899384867976178 26.696889194401873 23.248863586441082 84-85 23.184503838033354 26.64058589984675 26.521452241874922 23.65345802024497 86-87 23.061496432598865 26.502221944314474 26.947408594105433 23.488873028981224 88-89 23.177029628846576 26.583580139297343 26.74648074046988 23.492909491386197 90-91 23.102962149756607 26.856511946716644 26.778290040175946 23.2622358633508 92-93 23.146305440637324 26.63001618622758 26.834946700355218 23.38873167277988 94-95 23.00923110320125 26.69986337691604 26.743107253428068 23.547798266454638 96-97 23.036160769086646 26.68398145336529 26.7010808820204 23.57877689552766 98-99 22.935970478560453 26.60734956352074 26.645037424045505 23.81164253387331 100-101 22.90640728258971 26.65066297865431 26.623937523164038 23.818992215591944 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 103.0 1 84.5 2 118.5 3 191.5 4 159.0 5 144.0 6 328.0 7 688.0 8 1099.0 9 1236.0 10 1078.5 11 997.5 12 1074.5 13 1180.0 14 1300.0 15 1448.0 16 1567.0 17 1655.0 18 1733.0 19 1886.0 20 2155.0 21 2608.0 22 3245.0 23 3986.5 24 4955.0 25 6208.0 26 8132.0 27 10474.5 28 12797.5 29 15640.0 30 19161.0 31 23211.0 32 27415.0 33 31930.5 34 36907.0 35 41187.5 36 46118.0 37 51358.0 38 55598.5 39 59456.0 40 63475.5 41 69940.0 42 74946.0 43 78778.5 44 84401.5 45 89431.5 46 91903.5 47 92442.0 48 96248.5 49 96414.0 50 93115.0 51 90704.0 52 87870.5 53 86704.0 54 85385.5 55 81228.5 56 74450.5 57 67204.0 58 61281.5 59 55192.0 60 47362.0 61 39164.0 62 31688.5 63 24948.0 64 19549.0 65 15524.0 66 11476.5 67 9092.5 68 6568.0 69 4005.5 70 2841.0 71 2070.5 72 1609.0 73 1325.5 74 1023.5 75 822.5 76 522.0 77 233.5 78 125.5 79 65.5 80 38.5 81 20.0 82 8.5 83 6.0 84 4.0 85 1.0 86 1.5 87 1.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2638495153283389 2 0.021190656471032115 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.8145093975393E-4 12-13 1.0767609995443147E-4 14-15 0.0 16-17 6.891270397083615E-4 18-19 0.0 20-21 1.5074653993620406E-4 22-23 2.1535219990886296E-5 24-25 0.002562691178915469 26-27 0.0027995785988152184 28-29 0.002778043378824332 30-31 0.0014859301793711543 32-33 0.0017228175992709035 34-35 0.0017658880392526764 36-37 1.2921131994531778E-4 38-39 8.614087996354519E-5 40-41 0.0010121553395716558 42-43 0.003876339598359533 44-45 0.0052761288977671425 46-47 0.015096189213611291 48-49 0.014600879153820906 50-51 0.006891270397083614 52-53 0.008204918816527679 54-55 3.66098739845067E-4 56-57 0.0029718603587423086 58-59 0.001830493699225335 60-61 0.0018735641392071075 62-63 0.0 64-65 9.906201195807695E-4 66-67 0.0 68-69 0.0 70-71 2.7995785988152184E-4 72-73 0.0016151414993164721 74-75 0.0 76-77 0.19095279565918877 78-79 0.10349826727619953 80-81 0.001249042759471405 82-83 0.0 84-85 6.891270397083615E-4 86-87 0.12331066966781493 88-89 0.5867055334317062 90-91 0.3104948018285986 92-93 0.2949033025551969 94-95 0.0026703672788699006 96-97 0.0027134377188516735 98-99 0.003251818218623831 100-101 9.260144596081106E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2321778.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.53230548560323 #Duplication Level Percentage of deduplicated Percentage of total 1 69.89752222447369 33.92287901284898 2 15.301278196067578 14.852126154635034 3 5.8628015754847675 8.536058311887079 4 2.903242403018272 5.636041888081584 5 1.691381812388909 4.104332940582589 6 1.0114096594356263 2.9451625537691846 7 0.6835548155936958 2.32221437885839 8 0.479402901025428 1.8613222434600385 9 0.33754312222793104 1.4743571330277232 >10 1.6626551899493924 14.15882609352987 >50 0.10749718126966169 3.6130429315020094 >100 0.0588683578119441 5.121562697460764 >500 0.002131920939868088 0.7234932654863353 >1k 7.106403132891558E-4 0.7285803948704828 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4552 0.1960566427970288 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3356 0.1445443965788288 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2324 0.10009570251763951 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.1535219990886296E-5 0.0 18-19 0.0 0.0 0.0 4.307043998177259E-5 0.0 20-21 0.0 0.0 0.0 1.9381697991797665E-4 0.0 22-23 0.0 0.0 0.0 6.675918197174751E-4 0.0 24-25 0.0 0.0 0.0 0.0011629018795078598 0.0 26-27 0.0 0.0 0.0 0.001529000619352927 0.0 28-29 0.0 0.0 0.0 0.0020889163391159707 0.0 30-31 0.0 0.0 0.0 0.0036825226184415564 0.0 32-33 0.0 0.0 0.0 0.006439030777275002 0.0 34-35 0.0 0.0 0.0 0.010423046475588968 0.0 36-37 0.0 0.0 0.0 0.018046514352362715 0.0 38-39 0.0 0.0 0.0 0.031182998546803353 0.0 40-41 0.0 0.0 0.0 0.044276412301262225 0.0 42-43 0.0 0.0 0.0 0.058080488315420335 0.0 44-45 0.0 0.0 0.0 0.07453339638845746 0.0 46-47 0.0 0.0 0.0 0.09505646103977211 0.0 48-49 0.0 0.0 0.0 0.11928358352951919 0.0 50-51 0.0 0.0 0.0 0.14594418587823643 0.0 52-53 0.0 0.0 0.0 0.1733369857066438 0.0 54-55 0.0 0.0 0.0 0.20075132075504204 0.0 56-57 0.0 0.0 0.0 0.23389402432101605 0.0 58-59 0.0 0.0 0.0 0.2728512372845293 0.0 60-61 0.0 0.0 0.0 0.318936608065026 0.0 62-63 0.0 0.0 0.0 0.3665509794648756 0.0 64-65 0.0 0.0 0.0 0.41573742192405994 0.0 66-67 0.0 0.0 0.0 0.47399019199940734 0.0 68-69 0.0 0.0 0.0 0.5367438230528501 0.0 70-71 0.0 0.0 0.0 0.6082407534225924 0.0 72-73 0.0 0.0 0.0 0.6841524038904667 0.0 74-75 0.0 0.0 0.0 0.7608608574980036 0.0 76-77 0.0 0.0 0.0 0.8485092028609109 0.0 78-79 0.0 0.0 0.0 0.9506076808377029 0.0 80-81 0.0 0.0 0.0 1.0653257977291541 0.0 82-83 0.0 0.0 0.0 1.1861814523180079 0.0 84-85 0.0 0.0 0.0 1.3085230370862329 0.0 86-87 0.0 0.0 0.0 1.4379712444514505 0.0 88-89 0.0 0.0 0.0 1.5899668271471261 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6600 0.0 37.262745 1 GGTATCA 4400 0.0 35.460873 1 TCAACGC 7870 0.0 30.679224 4 ATCAACG 7865 0.0 30.638414 3 CAACGCA 8070 0.0 29.742558 5 AACGCAG 8175 0.0 29.476591 6 TATCAAC 8305 0.0 29.253103 2 GTACATG 9090 0.0 27.840435 1 TACATGG 9255 0.0 26.916868 2 ACATGGG 8995 0.0 26.578512 3 ACGCAGA 9675 0.0 24.85755 7 TACCTGG 2005 0.0 24.139454 2 CGCAGAG 9960 0.0 24.003386 8 CATGGGG 4915 0.0 23.934784 4 GTACCTG 2165 0.0 22.85092 1 GCAGAGT 11060 0.0 21.144293 9 CTCGCGC 490 0.0 19.846006 60-61 AGTACAT 6345 0.0 19.817822 2 GAGTACT 5140 0.0 19.795883 12-13 TCGCGGA 495 0.0 19.7926 82-83 >>END_MODULE