##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936308_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 414324 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.053595736669855 33.0 31.0 34.0 30.0 34.0 2 32.132253984804166 34.0 31.0 34.0 30.0 34.0 3 32.19784275108369 34.0 31.0 34.0 30.0 34.0 4 35.68385369903747 37.0 35.0 37.0 35.0 37.0 5 35.72947982738147 37.0 35.0 37.0 35.0 37.0 6 35.862769233739776 37.0 36.0 37.0 35.0 37.0 7 35.82719079754009 37.0 36.0 37.0 35.0 37.0 8 35.841899093463084 37.0 36.0 37.0 35.0 37.0 9 37.549910215193904 39.0 38.0 39.0 35.0 39.0 10-11 37.51095398770045 39.0 38.0 39.0 35.0 39.0 12-13 37.480374055087324 39.0 38.0 39.0 35.0 39.0 14-15 38.69550762205424 40.0 38.0 41.0 35.0 41.0 16-17 38.69656355895386 40.0 38.0 41.0 35.5 41.0 18-19 38.75016050240875 40.0 38.5 41.0 35.0 41.0 20-21 38.69954431797338 40.0 38.5 41.0 35.0 41.0 22-23 38.56395598613645 40.0 38.0 41.0 35.0 41.0 24-25 38.671164354466555 40.0 38.0 41.0 35.0 41.0 26-27 38.56747979841863 40.0 38.0 41.0 35.0 41.0 28-29 38.45697811374673 40.0 38.0 41.0 35.0 41.0 30-31 38.31133484905533 40.0 38.0 41.0 35.0 41.0 32-33 38.05320473832074 40.0 38.0 41.0 34.0 41.0 34-35 37.785602571900256 40.0 37.0 41.0 33.5 41.0 36-37 37.57068622623841 40.0 37.0 41.0 33.0 41.0 38-39 37.40010836929552 40.0 37.0 41.0 33.0 41.0 40-41 37.301795213407864 40.0 37.0 41.0 33.0 41.0 42-43 37.11362967146485 40.0 37.0 41.0 33.0 41.0 44-45 36.87245730394571 40.0 37.0 41.0 32.0 41.0 46-47 36.514702261997854 40.0 36.0 41.0 31.0 41.0 48-49 36.25781393305722 40.0 36.5 41.0 30.0 41.0 50-51 35.87787818229212 39.5 35.5 40.5 29.5 41.0 52-53 35.95731239319953 39.5 36.0 40.5 30.0 41.0 54-55 36.0981900638148 40.0 36.0 41.0 29.5 41.0 56-57 35.974676822969464 40.0 36.0 41.0 28.5 41.0 58-59 35.82377076877033 40.0 36.0 41.0 28.0 41.0 60-61 35.558997306455815 39.5 35.0 41.0 28.0 41.0 62-63 35.31892311331229 39.0 35.0 41.0 27.0 41.0 64-65 35.027056120330954 39.0 35.0 41.0 26.0 41.0 66-67 34.67483901487725 38.5 35.0 40.0 26.0 41.0 68-69 34.25666507371043 37.5 35.0 40.0 25.5 41.0 70-71 33.79389077147353 37.0 34.5 39.0 24.0 41.0 72-73 33.33970757185198 36.5 34.0 39.0 22.5 41.0 74-75 32.9013079136135 36.0 34.0 38.5 21.5 40.0 76-77 32.49247086820942 35.5 34.0 37.5 20.0 39.5 78-79 32.04507583437117 35.0 33.5 37.0 16.0 39.0 80-81 31.58533538969502 35.0 33.0 36.5 9.0 39.0 82-83 31.17237355306475 35.0 32.5 36.0 4.5 37.5 84-85 30.79102345024667 35.0 32.0 36.0 2.0 37.0 86-87 30.52773191994671 35.0 32.0 35.0 2.0 37.0 88-89 30.137035267085665 35.0 32.0 35.0 2.0 36.0 90-91 29.771792124038193 35.0 31.0 35.0 2.0 36.0 92-93 29.46533268649656 34.5 31.0 35.0 2.0 36.0 94-95 29.10628035064346 34.0 31.0 35.0 2.0 35.5 96-97 28.725934292968788 34.0 30.0 35.0 2.0 35.0 98-99 28.39245493864705 34.0 30.0 35.0 2.0 35.0 100-101 25.463806103435957 31.5 24.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07581407685911756 1101 2 0.018382649845484877 1101 3 0.07238271877190527 1101 4 -0.2603940306074577 1101 5 -0.0521297764585853 1101 6 0.040001835151834086 1101 7 0.023453839465886972 1101 8 0.23298159686587638 1101 9 0.16284037219918446 1101 10-11 0.27781182489145095 1101 12-13 0.21424075455647795 1101 14-15 0.3462210448265992 1101 16-17 0.09462979839432961 1101 18-19 0.18265864357488937 1101 20-21 0.22889638938445955 1101 22-23 0.360002119764431 1101 24-25 0.2529849870770633 1101 26-27 0.11810955364411058 1101 28-29 0.05852150449859295 1101 30-31 0.2962842192529678 1101 32-33 0.4705702041753739 1101 34-35 0.25761424095367147 1101 36-37 0.146592010451414 1101 38-39 0.5745596431357782 1101 40-41 0.32806117708993554 1101 42-43 0.09211934054879833 1101 44-45 0.4168008313513951 1101 46-47 0.47340983528750513 1101 48-49 0.40758740447965636 1101 50-51 0.5801581697485929 1101 52-53 0.5321050563596685 1101 54-55 0.5348158867953927 1101 56-57 0.5208489506339689 1101 58-59 0.4267414927056734 1101 60-61 0.3919807799683497 1101 62-63 0.5212827251450634 1101 64-65 0.40671069010815586 1101 66-67 0.5517951647186621 1101 68-69 0.591662837429368 1101 70-71 0.44699073819647595 1101 72-73 0.5856333019761948 1101 74-75 0.608187880476919 1101 76-77 0.48800877704292844 1101 78-79 0.5939352998127916 1101 80-81 0.8710499211180966 1101 82-83 1.0378488936897803 1101 84-85 0.7398186433639218 1101 86-87 0.5815652072827504 1101 88-89 0.3492412090785315 1101 90-91 0.31903535008093087 1101 92-93 0.4528627112130117 1101 94-95 0.6092629957366427 1101 96-97 0.848208707240456 1101 98-99 0.38033106260616023 1101 100-101 0.5985948455752066 1102 1 0.14480588507927195 1102 2 0.2223260520937913 1102 3 0.19119898885240616 1102 4 0.17089785917587363 1102 5 0.2688922455989342 1102 6 0.29676625942219914 1102 7 0.155532991208851 1102 8 0.259631478067341 1102 9 0.37697917144738113 1102 10-11 0.23887427391446892 1102 12-13 0.2554744862490921 1102 14-15 0.4389636952751914 1102 16-17 0.26151514893867756 1102 18-19 -0.016169329048260295 1102 20-21 0.17422246477720194 1102 22-23 0.060461355907563075 1102 24-25 0.13741351291130854 1102 26-27 0.16674603011491484 1102 28-29 0.04194290242050869 1102 30-31 0.17641294722204037 1102 32-33 0.18172130882447135 1102 34-35 0.04609078267500877 1102 36-37 -0.02764289149546073 1102 38-39 -0.10127106008065567 1102 40-41 -0.028955029158524326 1102 42-43 0.1132637073703151 1102 44-45 0.34789767875733446 1102 46-47 0.4486039911086337 1102 48-49 0.35409259969934226 1102 50-51 0.10982234223698129 1102 52-53 0.085830365767805 1102 54-55 -0.11596960205172735 1102 56-57 -0.05979220685389208 1102 58-59 -0.0798456203125113 1102 60-61 0.20045803093469772 1102 62-63 0.2075925233648519 1102 64-65 0.08654862762367088 1102 66-67 0.07588289832623474 1102 68-69 0.37248839861948113 1102 70-71 0.4634097601476128 1102 72-73 0.45404175617670006 1102 74-75 0.5667413752816941 1102 76-77 0.6130486720746688 1102 78-79 0.35582401927092633 1102 80-81 -0.016206719188293306 1102 82-83 0.17082346725621989 1102 84-85 0.25390602234898907 1102 86-87 0.17485752261308107 1102 88-89 4.965727439092404E-4 1102 90-91 0.12802680367857633 1102 92-93 0.0832751371855629 1102 94-95 0.42862715758635517 1102 96-97 0.258834970583127 1102 98-99 0.23068036577294038 1102 100-101 0.3648188251489515 1103 1 0.21514700695502853 1103 2 0.2550542129665416 1103 3 0.3131154587701772 1103 4 0.3094063668694318 1103 5 0.3304244445731257 1103 6 0.1123080651699766 1103 7 0.12462034771785113 1103 8 0.31022172474974496 1103 9 0.0765965287705086 1103 10-11 0.223206277496665 1103 12-13 0.20821811565559045 1103 14-15 0.15454213702394526 1103 16-17 0.3803379121072865 1103 18-19 0.45032908339170064 1103 20-21 0.2735304514254224 1103 22-23 0.26210183461797243 1103 24-25 0.3923308783757733 1103 26-27 0.3954360895466422 1103 28-29 0.24002154770260375 1103 30-31 0.03624360748418809 1103 32-33 0.039329385027357944 1103 34-35 0.060582356394782266 1103 36-37 0.1693000377563436 1103 38-39 0.11402650566061823 1103 40-41 0.3967512315269843 1103 42-43 0.18784646402585992 1103 44-45 -0.00691384937097439 1103 46-47 0.2907476042324575 1103 48-49 0.296211788822518 1103 50-51 0.05602441013569859 1103 52-53 -0.14126399228803876 1103 54-55 -0.20053363364009869 1103 56-57 -0.0038205386946472686 1103 58-59 0.049756268476869536 1103 60-61 0.042369616052340575 1103 62-63 0.21521688223484148 1103 64-65 0.09149855842233023 1103 66-67 0.10407633641138858 1103 68-69 0.2978323699619949 1103 70-71 0.13949052217643043 1103 72-73 0.07001505260264906 1103 74-75 0.026022495529190337 1103 76-77 -0.15302639102066706 1103 78-79 -0.15637576905439943 1103 80-81 -0.27884206885856955 1103 82-83 -0.16345154699111575 1103 84-85 -0.18231477061689816 1103 86-87 -0.33722421637576616 1103 88-89 -0.14002691500338216 1103 90-91 -0.08576461535585977 1103 92-93 -0.16917133354634117 1103 94-95 -0.16186376482673737 1103 96-97 -0.016618908762314533 1103 98-99 -0.09665416162264862 1103 100-101 -0.030589462728602967 1104 1 0.2480631668671478 1104 2 0.1808653471813031 1104 3 0.3712189543957436 1104 4 0.2536019021983975 1104 5 0.17077126084681282 1104 6 0.11252776778679419 1104 7 0.11664043481424358 1104 8 0.07160114332246792 1104 9 0.04139703094791969 1104 10-11 -0.023523684447710025 1104 12-13 0.10286286437072079 1104 14-15 0.09321764410543665 1104 16-17 0.3026377277140213 1104 18-19 0.08144446649813375 1104 20-21 0.36110156053130993 1104 22-23 0.2762059579653027 1104 24-25 0.23535335866139206 1104 26-27 0.16578800605964972 1104 28-29 0.39079246849195926 1104 30-31 0.06116023818763239 1104 32-33 0.31491885499479366 1104 34-35 0.3668517274960479 1104 36-37 0.4672167861576213 1104 38-39 0.12732636049995705 1104 40-41 -0.00848632192744958 1104 42-43 0.026510201324306593 1104 44-45 0.12909384022061232 1104 46-47 0.2506754162297895 1104 48-49 0.1695924628387644 1104 50-51 0.36368261563611526 1104 52-53 0.3153133548028606 1104 54-55 0.32483417208000986 1104 56-57 0.3644755890467337 1104 58-59 0.24655881075000252 1104 60-61 0.3675647982878161 1104 62-63 0.5511971089993253 1104 64-65 0.4319042236112409 1104 66-67 0.5067441539012876 1104 68-69 -0.07985996790924332 1104 70-71 0.0363322970597153 1104 72-73 -0.08024584425481862 1104 74-75 0.02034161358011488 1104 76-77 0.1085805766908976 1104 78-79 0.016671427775605707 1104 80-81 0.09581366498739996 1104 82-83 0.31623380747551977 1104 84-85 0.019254533788920725 1104 86-87 0.320243710965439 1104 88-89 0.3898447943614407 1104 90-91 0.629838193699026 1104 92-93 0.5230645329058525 1104 94-95 0.4330791517255008 1104 96-97 0.9164581048514044 1104 98-99 1.0274464307897624 1104 100-101 1.3597710849586342 1105 1 -0.18007515474444702 1105 2 -0.15339649125881039 1105 3 -0.03425050496804971 1105 4 -0.06092279039459214 1105 5 -0.2650233804988531 1105 6 -0.10830511668357445 1105 7 0.06450051268555512 1105 8 -0.2289036351444267 1105 9 -0.3228898925196617 1105 10-11 -0.29304537607115577 1105 12-13 -0.07752618439888437 1105 14-15 -0.25057419633532163 1105 16-17 -0.2583088858072742 1105 18-19 -0.14532495131377487 1105 20-21 -0.18533267206691306 1105 22-23 0.06040546857641971 1105 24-25 -0.06104239996832206 1105 26-27 0.0918410385778472 1105 28-29 -0.09569162636945094 1105 30-31 0.09916867168153232 1105 32-33 -0.13328364119627878 1105 34-35 -0.115415620416357 1105 36-37 0.04406853826213819 1105 38-39 -0.19231152250839756 1105 40-41 -0.22677222968226118 1105 42-43 -0.12012178747207969 1105 44-45 -0.517765144989994 1105 46-47 -0.33749922480807726 1105 48-49 -0.12174012153464986 1105 50-51 -0.2715687732741472 1105 52-53 -0.2566791350784925 1105 54-55 -0.2276404735747377 1105 56-57 0.01829836255817696 1105 58-59 0.13750819439702866 1105 60-61 0.12021180732150327 1105 62-63 0.03380687523149106 1105 64-65 0.15671916382269302 1105 66-67 0.10677262463798343 1105 68-69 0.09607764270341335 1105 70-71 0.09098456933229215 1105 72-73 0.26642000163385404 1105 74-75 0.3089810201576313 1105 76-77 0.47191719313621405 1105 78-79 0.45610185914573975 1105 80-81 0.2594934692318489 1105 82-83 0.3517068064444153 1105 84-85 0.4002430069879601 1105 86-87 0.08764933432068034 1105 88-89 0.19289270214703436 1105 90-91 0.35229467964063943 1105 92-93 0.2697711612011453 1105 94-95 0.06644531617862981 1105 96-97 0.15383864317917073 1105 98-99 0.10214748805441687 1105 100-101 -0.10691815310289243 1106 1 0.2810744867272845 1106 2 0.20830563640076605 1106 3 0.25662759541986446 1106 4 0.3302109817930159 1106 5 0.352615034923744 1106 6 0.3394731835397735 1106 7 0.25416784547032023 1106 8 0.14402454165115586 1106 9 0.29244159919304735 1106 10-11 0.3478092142662135 1106 12-13 0.3204034617748519 1106 14-15 0.3328497486511637 1106 16-17 0.29504423515193423 1106 18-19 0.10623258953494741 1106 20-21 0.17293111070021894 1106 22-23 0.23539371355114724 1106 24-25 0.16530061166914578 1106 26-27 0.3603184689293215 1106 28-29 0.36745636939873805 1106 30-31 0.32836227694778586 1106 32-33 0.5022077188601415 1106 34-35 0.3088967106051541 1106 36-37 0.2650409727278742 1106 38-39 0.258486349832026 1106 40-41 0.3657765536726174 1106 42-43 0.2517998652844753 1106 44-45 0.24631884466558063 1106 46-47 0.27405596503557916 1106 48-49 0.5331911115675041 1106 50-51 0.7813802686912936 1106 52-53 0.7554358878438094 1106 54-55 0.737131185059333 1106 56-57 0.7502250112004702 1106 58-59 0.7606567779164806 1106 60-61 0.6461904131755034 1106 62-63 0.45720819179432226 1106 64-65 0.6362580729563518 1106 66-67 0.735534232725449 1106 68-69 0.7204237694225881 1106 70-71 0.6509866264617159 1106 72-73 0.5934080702832034 1106 74-75 0.6677590852804656 1106 76-77 0.7942840674749831 1106 78-79 0.6671633379279172 1106 80-81 0.7893291927536517 1106 82-83 0.9082558248998076 1106 84-85 0.9564026402311683 1106 86-87 0.9404690710081134 1106 88-89 0.8760653834682977 1106 90-91 0.949427922852113 1106 92-93 0.7196359784105049 1106 94-95 0.8964970382898372 1106 96-97 0.6219898715793128 1106 98-99 0.8352569861148389 1106 100-101 1.207727139009485 1107 1 0.26199074346882156 1107 2 0.3284976591590194 1107 3 0.3962885922787507 1107 4 0.451373489942128 1107 5 0.2738353276991816 1107 6 0.24066147252217007 1107 7 0.21134795570560527 1107 8 0.2777292770271984 1107 9 0.4452967523963878 1107 10-11 0.420766009145602 1107 12-13 0.4399102181311676 1107 14-15 0.5695407638825927 1107 16-17 0.3722756107223759 1107 18-19 0.5382689790329565 1107 20-21 0.6995416719519199 1107 22-23 0.7177101362382814 1107 24-25 0.7701723001655623 1107 26-27 0.6783228250011533 1107 28-29 0.7027701843694061 1107 30-31 0.5458399039257955 1107 32-33 0.6756430889780844 1107 34-35 0.7038990812564876 1107 36-37 0.7443755605308766 1107 38-39 0.7401397309734961 1107 40-41 0.697641811777288 1107 42-43 0.554365354527647 1107 44-45 0.7836899404991584 1107 46-47 0.7534609315501299 1107 48-49 0.8938264461256722 1107 50-51 0.8177216128505975 1107 52-53 0.6077924634379528 1107 54-55 0.6131350077346056 1107 56-57 0.85890455840606 1107 58-59 0.8301242703600309 1107 60-61 0.6461163303212345 1107 62-63 0.8381051869937579 1107 64-65 0.4917928029984253 1107 66-67 0.510922426881784 1107 68-69 0.4229255474110829 1107 70-71 0.6477529098730841 1107 72-73 0.376578668279997 1107 74-75 0.5050212793182851 1107 76-77 0.31888698337613164 1107 78-79 0.46235387902908087 1107 80-81 0.49971338643586805 1107 82-83 0.6602449495368035 1107 84-85 0.7407030017554987 1107 86-87 0.5876531817175312 1107 88-89 0.8884520367012776 1107 90-91 0.8721780265681076 1107 92-93 0.7974377919587781 1107 94-95 0.9080095138424937 1107 96-97 0.9986308068012626 1107 98-99 0.6151901633802943 1107 100-101 0.47536997075250653 1108 1 -0.010627640932291627 1108 2 0.0866035087411916 1108 3 0.045404191164543306 1108 4 0.0459556626440758 1108 5 0.034051205136506724 1108 6 0.054526375029134044 1108 7 -0.07657683538074167 1108 8 -0.08847545834884585 1108 9 0.12244159919304565 1108 10-11 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-0.8551889994633584 2316 56-57 -0.8211457287731676 2316 58-59 -1.0830120226860345 2316 60-61 -1.250787557074915 2316 62-63 -1.0708232523781476 2316 64-65 -0.7840841413159154 2316 66-67 -0.8060445659941919 2316 68-69 -0.9438602637161821 2316 70-71 -0.8831194740843245 2316 72-73 -0.9397942456648352 2316 74-75 -0.6254295797543676 2316 76-77 -0.8744647557221512 2316 78-79 -1.0855668447118845 2316 80-81 -1.082693496005522 2316 82-83 -1.233151160601981 2316 84-85 -1.1600537709908245 2316 86-87 -1.3086812773255367 2316 88-89 -1.259483477386528 2316 90-91 -1.3789965734762646 2316 92-93 -1.410913823886613 2316 94-95 -1.1345423139978692 2316 96-97 -1.5136649015330939 2316 98-99 -1.4480065235820128 2316 100-101 -1.5534736706308827 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2582.0 3 342.0 4 233.0 5 250.0 6 261.0 7 304.0 8 394.0 9 427.0 10 624.0 11 854.0 12 1364.0 13 2256.0 14 2483.0 15 2357.0 16 3010.0 17 4746.0 18 3922.0 19 1986.0 20 1800.0 21 1650.0 22 1731.0 23 1879.0 24 2047.0 25 2264.0 26 2645.0 27 3086.0 28 3864.0 29 4656.0 30 5578.0 31 7164.0 32 9335.0 33 12292.0 34 17533.0 35 30879.0 36 42448.0 37 80122.0 38 126633.0 39 28309.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.470860669691476 22.899275833009362 14.50024267683414 20.12962082046502 2 13.781750332498968 24.833991894507914 39.60467019240285 21.779587580590267 3 18.409917618690155 29.963335127554654 32.08647020345504 19.54027705030015 4 10.181989331659869 19.29642055465714 43.379112258936544 27.142477854746446 5 9.30248275395971 42.455430632057116 36.79863286812874 11.443453745854432 6 25.018766367772532 42.84572959534642 18.217496771702006 13.918007265179035 7 23.488136329624176 36.83868787564267 24.646020903232035 15.027154891501121 8 23.228234317218472 34.8885766976605 22.45169886742172 19.431490117699312 9 24.671822873668102 18.66590385701664 24.654683796088428 32.00758947322683 10-11 21.82392183743809 28.871803164734168 29.990393805694204 19.313881192133543 12-13 24.40858592868393 28.14709769299934 29.271330553426704 18.17298582489002 14-15 21.362950625776943 30.01412071400123 29.334998853446297 19.287929806775526 16-17 17.393461262701003 32.9032273639131 30.397668978536547 19.30564239484935 18-19 16.92237134305301 33.071835473592735 32.90516076083808 17.100632422516174 20-21 18.656228276820887 30.806726461728584 32.07632559666332 18.46071966478721 22-23 18.31853867831733 30.367442506019444 32.002437949949616 19.311580865713612 24-25 19.47465495638823 31.096293542367277 31.755358828457137 17.673692672787364 26-27 18.150088944994096 30.23091906608963 34.03720966157456 17.581782327341717 28-29 17.426406777492527 31.412770199889685 33.736106031693154 17.424716990924637 30-31 18.18367250874743 30.789628365452508 32.705071276925736 18.321627848874332 32-33 17.54421537176637 31.7791874557967 33.21553832079348 17.461058851643458 34-35 18.687476781628114 30.51296417080009 32.76830757278922 18.03125147478258 36-37 18.866014011115276 29.64647217967794 32.90477801586407 18.58273579334272 38-39 17.823774005434988 31.272153149733768 33.13692844418952 17.767144400641723 40-41 17.020840484123124 31.572915011625213 33.8215478210457 17.584696683205962 42-43 17.453512640996195 31.315859365809708 31.768939058598388 19.461688934595713 44-45 17.856419449562207 30.382511937957794 32.14461151453477 19.61645709794523 46-47 19.025363593357675 30.66447520044638 32.17137104110819 18.13879016508776 48-49 18.583476071017863 31.13703302941933 31.75873045140692 18.520760448155894 50-51 17.374710356791905 30.950029722549104 32.681216562132256 18.99404335852673 52-53 17.802728878365002 32.16100091221396 31.49430836713701 18.541961842284028 54-55 18.255276464801938 30.558193636342683 31.359563740277004 19.826966158578372 56-57 19.2670197708421 30.88549746087615 30.451535906134286 19.39594686214747 58-59 19.405984543809517 29.859894921190893 31.399449864406613 19.33467067059297 60-61 18.893899114168452 31.497229957212298 31.456237204924953 18.152633723694294 62-63 18.08012515386528 31.313327310101446 32.123067967523056 18.48347956851022 64-65 18.035715801871056 31.801327668163008 31.55025210654822 18.612704423417707 66-67 18.566849900137154 31.257738309619697 29.879546124266493 20.295865665976663 68-69 17.996050662144818 30.67589957813891 31.227788006918615 20.10026175279765 70-71 19.67642140164033 30.176172187181283 31.227602532649517 18.91980387852887 72-73 19.15269079071843 29.478870027992183 31.814058186863566 19.55438099442582 74-75 18.181564560911085 32.181416578625296 30.295855404325206 19.341163456138414 76-77 18.32566206390501 30.428438881510722 31.43971231223583 19.806186742348437 78-79 18.210452268620152 30.7377715249057 31.525390838194806 19.526385368279342 80-81 19.48074000492431 31.019756671166466 30.113732153924712 19.385771169984512 82-83 19.593550562397365 30.031800860151414 31.15744457893575 19.217203998515476 84-85 18.25417136148242 30.35666853852862 31.177053479055562 20.2121066209334 86-87 18.642805596152797 30.935663648657663 30.924262730519892 19.497268024669644 88-89 18.967796873332105 30.42254170346146 30.450683655348428 20.158977767858012 90-91 18.990818898000832 29.00667683418811 31.336470947945443 20.666033319865615 92-93 20.355554045840606 30.64257646874708 30.455627927515888 18.546241557896423 94-95 19.466784393583076 29.254087594162332 31.29749050038387 19.98163751187073 96-97 18.8389734734759 29.326384409058498 31.53644304970146 20.298199067764138 98-99 19.31162754605296 30.357547738303523 30.13384548418504 20.196979231458478 100-101 18.93805738952244 29.512983273009464 30.63834173017345 20.91061760729464 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2832.0 1 1664.5 2 728.5 3 917.0 4 603.5 5 567.0 6 1270.5 7 1991.0 8 2388.0 9 2199.0 10 1506.5 11 1071.5 12 1023.0 13 993.0 14 926.0 15 879.5 16 858.5 17 860.0 18 809.0 19 787.5 20 880.5 21 973.5 22 1134.5 23 1389.5 24 1527.0 25 1863.0 26 2993.5 27 4281.0 28 4447.0 29 4986.0 30 6770.0 31 8395.0 32 11001.0 33 14249.5 34 16825.0 35 19422.5 36 23471.5 37 26888.5 38 25635.0 39 22468.0 40 19744.0 41 16445.0 42 12576.5 43 12129.0 44 13215.5 45 12545.0 46 11256.0 47 13467.0 48 22307.0 49 20865.0 50 11391.5 51 6595.0 52 3757.0 53 2703.0 54 2022.0 55 1756.0 56 1628.5 57 1423.0 58 1262.5 59 1112.5 60 947.5 61 781.5 62 502.5 63 229.5 64 131.0 65 96.5 66 60.0 67 36.5 68 35.0 69 54.0 70 50.5 71 27.0 72 18.5 73 14.5 74 12.0 75 9.0 76 7.0 77 5.5 78 4.0 79 3.0 80 2.0 81 2.5 82 2.0 83 1.5 84 2.0 85 1.5 86 2.0 87 4.0 88 3.0 89 1.0 90 0.5 91 0.5 92 1.5 93 2.0 94 1.5 95 3.0 96 2.5 97 1.5 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046581902086289956 2 0.00893020920825248 3 0.007482067174481806 4 0.0033789980787982347 5 0.006275282146339579 6 0.004585783106940462 7 0.008206138191367143 8 0.011585136270165377 9 0.015929562371477395 10-11 0.0019308560450275632 12-13 0.010016315733580484 14-15 0.00941292321950937 16-17 0.020515345478417858 18-19 0.010619708247651596 20-21 0.004827140112568907 22-23 0.010016315733580484 24-25 0.02437705756847298 26-27 0.005551211129454244 28-29 0.0170156688968054 30-31 0.0143607418348925 32-33 0.010861065253280042 34-35 0.27213002384607216 36-37 0.4847655458047325 38-39 0.48162790473156275 40-41 0.5004537511705814 42-43 0.49104082795107207 44-45 0.5040741062550081 46-47 0.5114354949266757 48-49 0.5179521340786437 50-51 0.5259169152643824 52-53 0.5165039920448731 54-55 0.505039534277522 56-57 0.5009364651818383 58-59 0.4976781456058544 60-61 0.4962300035720837 62-63 0.4903167569341868 64-65 0.5062463193056641 66-67 0.4847655458047325 68-69 0.5075737828366206 70-71 0.48959268591730143 72-73 0.49864357362836814 74-75 0.5098666743900909 76-77 0.49997103715932456 78-79 0.49936764464525346 80-81 0.502867321226866 82-83 0.49985035865651034 84-85 0.5016605361987237 86-87 0.5011778221874669 88-89 0.5136077079773318 90-91 0.537622730037362 92-93 0.5257962367615683 94-95 0.4997296801536962 96-97 0.4783695851555787 98-99 0.46956005445014043 100-101 0.47462855156833783 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 414324.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.572846371438775 #Duplication Level Percentage of deduplicated Percentage of total 1 71.14618657130526 13.925333796738784 2 9.989518465996671 3.9104662051920718 3 4.086565139651026 2.399571349958004 4 2.4983044577347555 1.9559571736129215 5 1.744867131142487 1.707600814821251 6 1.2688821752265862 1.4901381527500217 7 0.9458043035945496 1.2958457632191231 8 0.7448054750601147 1.166237051196648 9 0.6658856896232813 1.1729950473542443 >10 5.276527529440779 21.463878510537647 >50 0.913743140760836 12.723858622720382 >100 0.6572538380911277 23.39159691449204 >500 0.04069301436586719 5.088529749664514 >1k 0.019729946359208335 7.001525376275572 >5k 0.001233121647450521 1.306465471466775 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5413 1.306465471466775 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3721 0.8980894179434453 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2929 0.7069346694857165 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2912 0.7028316003900329 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2716 0.6555256272868576 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2234 0.539191550573947 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1978 0.47740415713306494 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1592 0.38424035296048503 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1379 0.33283131076162614 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1369 0.3304177407053417 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1363 0.32896959867157105 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1234 0.2978345449455016 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1226 0.295903688900474 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1226 0.295903688900474 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1077 0.25994149506183567 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1052 0.2539075699211245 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1001 0.2415983626340738 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 929 0.22422065822882575 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 870 0.20998059489674747 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 861 0.20780838184609146 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 814 0.19646460258155454 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 768 0.18536218032264604 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 767 0.1851208233170176 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 748 0.18053504021007713 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 739 0.17836282715942114 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 727 0.17546654309187978 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 713 0.17208754501308154 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 698 0.16846718992865486 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 697 0.16822583292302642 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 685 0.1653295488554851 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 655 0.1580888386866317 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 643 0.15519255461909037 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 613 0.147951844450237 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 579 0.13974570625886987 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 577 0.139262992247613 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 575 0.13878027823635608 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 572 0.13805620721947076 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 561 0.13540128015755787 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 558 0.13467720914067252 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 548 0.13226363908438807 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 542 0.13081549705061737 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 534 0.12888464100558983 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 527 0.12719514196619072 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 527 0.12719514196619072 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 524 0.12647107094930538 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 522 0.1259883569380485 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 504 0.12164393083673648 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 503 0.12140257383110802 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 502 0.12116121682547956 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 501 0.12091985981985112 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 496 0.1197130747917089 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 489 0.11802357575230978 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 487 0.1175408617410529 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 485 0.117058147729796 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 481 0.11609271970728222 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 466 0.11247236462285555 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 464 0.11198965061159866 No Hit ACCTAAAACCTGGTGAACTACGACTGCTAGAAGTTGATAACCGAGTCGTT 459 0.11078286558345643 No Hit GCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGT 441 0.1064384394821444 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 437 0.10547301145963062 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 433 0.10450758343711684 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 429 0.10354215541460306 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 422 0.10185265637520395 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 419 0.10112858535831862 No Hit CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC 415 0.10016315733580483 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.6203550844266804E-4 0.0 16-17 0.0 0.0 0.0 0.0012067850281422268 0.0 18-19 0.0 0.0 0.0 0.0028962840675413443 0.0 20-21 0.0 0.0 0.0 0.005068497118197353 0.0 22-23 0.0 0.0 0.0 0.008688852202624034 0.0 24-25 0.0 0.0 0.0 0.014602098840520945 0.0 26-27 0.0 0.0 0.0 0.019549917455904075 0.0 28-29 0.0 0.0 0.0 0.03197980324576901 0.0 30-31 0.0 0.0 0.0 0.06649385505063671 0.0 32-33 0.0 0.0 0.0 0.1293673550168467 0.0 34-35 0.0 0.0 0.0 0.21613519854027285 0.0 36-37 0.0 0.0 0.0 0.34670933858526176 0.0 38-39 0.0 0.0 0.0 0.5254342012531256 0.0 40-41 0.0 0.0 0.0 0.7232262673656366 0.0 42-43 0.0 0.0 0.0 0.8972446684237456 0.0 44-45 0.0 0.0 0.0 1.0917784149602725 0.0 46-47 0.0 0.0 0.0 1.3379625607012868 0.0 48-49 0.0 0.0 0.0 1.592714880142111 0.0 50-51 0.0 0.0 0.0 1.830572209188944 0.0 52-53 0.0 0.0 0.0 2.0247439202170283 0.0 54-55 0.0 0.0 0.0 2.2534296830499803 0.0 56-57 0.0 0.0 0.0 2.517836282715942 0.0 58-59 0.0 0.0 0.0 2.7890008785395004 0.0 60-61 0.0 0.0 0.0 3.0852666029484173 0.0 62-63 0.0 0.0 0.0 3.3569139127832326 0.0 64-65 0.0 0.0 0.0 3.6393016093685135 0.0 66-67 0.0 0.0 0.0 3.9710468136048114 0.0 68-69 0.0 0.0 0.0 4.348287813402072 0.0 70-71 0.0 0.0 0.0 4.765835433139282 0.0 72-73 0.0 0.0 0.0 5.137042507795831 0.0 74-75 0.0 0.0 0.0 5.525989322366071 0.0 76-77 0.0 0.0 0.0 5.9413647290526255 0.0 78-79 0.0 0.0 0.0 6.458954827622827 0.0 80-81 0.0 0.0 0.0 7.025661076838416 0.0 82-83 0.0 0.0 0.0 7.542285747386104 0.0 84-85 0.0 0.0 0.0 8.085218331547292 0.0 86-87 0.0 0.0 0.0 8.657475791892335 0.0 88-89 0.0 0.0 0.0 9.277039225340555 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGGACC 25 0.005090199 56.797535 6 CATGGGT 185 0.0 43.49361 4 ATGGGTA 170 0.0 38.978703 5 GTAAGCG 25 0.0016054036 38.04877 94-95 TGGGTAC 50 0.0016430938 37.865025 6 GATGACC 100 3.119021E-8 37.865025 9 ACTCCCG 50 0.0016430938 37.865025 6 GTACAGA 70 2.0914237E-4 33.816223 1 TGGGTAT 110 2.8046034E-6 30.119904 6 GGGATGA 270 0.0 29.801178 7 CATTCCG 80 4.5832474E-4 29.58205 9 CATGGGG 665 0.0 29.181694 4 GTGGTAC 65 0.005935228 29.133974 1 CGGTATA 75 3.2890966E-8 28.54004 34-35 GGATGAC 260 0.0 27.306507 8 GTCTAGA 140 5.961265E-7 27.052979 1 ACCCTAC 70 0.008532286 27.046448 7 CCAGGAC 105 7.064405E-5 27.046446 3 TACCTCC 70 0.008537312 27.04318 2 CCTTTAG 90 9.112598E-4 26.301508 1 >>END_MODULE