##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936308_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 414324 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.39313677218795 34.0 31.0 34.0 31.0 34.0 2 32.52356851159962 34.0 31.0 34.0 31.0 34.0 3 32.61171209005513 34.0 31.0 34.0 31.0 34.0 4 36.102366746797195 37.0 35.0 37.0 35.0 37.0 5 36.09907705081048 37.0 35.0 37.0 35.0 37.0 6 36.16347834062231 37.0 36.0 37.0 35.0 37.0 7 36.119208638649944 37.0 36.0 37.0 35.0 37.0 8 36.113809482434036 37.0 36.0 37.0 35.0 37.0 9 37.85135304737355 39.0 38.0 39.0 35.0 39.0 10-11 37.79869256910051 39.0 38.0 39.0 35.0 39.0 12-13 37.78271714889796 39.0 38.0 39.0 35.0 39.0 14-15 39.007835655187726 40.0 38.0 41.0 35.5 41.0 16-17 38.95840694722005 40.0 38.0 41.0 35.5 41.0 18-19 39.07568352303994 40.0 38.5 41.0 35.5 41.0 20-21 39.044234705206556 40.0 38.5 41.0 35.0 41.0 22-23 38.9181473918962 40.0 38.0 41.0 35.0 41.0 24-25 39.030222724244794 40.0 38.5 41.0 35.0 41.0 26-27 38.87961112559253 40.0 38.0 41.0 35.0 41.0 28-29 38.77887957250847 40.0 38.0 41.0 35.0 41.0 30-31 38.644394242187275 40.0 38.0 41.0 35.0 41.0 32-33 38.399554454967614 40.0 38.0 41.0 34.0 41.0 34-35 38.23100037651693 40.0 37.5 41.0 34.0 41.0 36-37 38.056716482752634 40.0 37.0 41.0 33.5 41.0 38-39 37.89013911817804 40.0 37.0 41.0 33.0 41.0 40-41 37.82042194031724 40.0 37.0 41.0 33.0 41.0 42-43 37.75923552582037 40.0 37.0 41.0 33.0 41.0 44-45 37.53865091088134 40.0 37.0 41.0 33.0 41.0 46-47 37.23998851140653 40.0 37.0 41.0 33.0 41.0 48-49 36.9443225591566 40.0 37.0 41.0 32.0 41.0 50-51 36.722435823172205 40.0 37.0 41.0 31.0 41.0 52-53 36.5939578687211 40.0 37.0 41.0 31.0 41.0 54-55 36.47623840279588 40.0 36.5 41.0 31.0 41.0 56-57 36.25400411272338 40.0 36.0 41.0 30.0 41.0 58-59 36.055997238875854 40.0 36.0 41.0 29.5 41.0 60-61 35.794932226952824 39.0 35.0 41.0 28.0 41.0 62-63 35.55345936996167 39.0 35.0 41.0 28.0 41.0 64-65 35.21282498720808 39.0 35.0 40.0 28.0 41.0 66-67 34.85138683735434 38.0 35.0 40.0 27.0 41.0 68-69 34.44053325416823 37.0 35.0 40.0 26.0 41.0 70-71 34.02122976221508 37.0 34.0 39.0 26.0 41.0 72-73 33.57774471186801 36.0 34.0 39.0 26.0 40.5 74-75 33.120980199071255 36.0 34.0 38.5 25.5 40.0 76-77 32.33634788233363 35.0 32.5 37.0 23.5 39.0 78-79 32.34046181249457 35.0 33.0 37.0 23.5 39.0 80-81 32.09987835606916 35.0 33.0 36.5 23.0 39.0 82-83 31.766222811133318 35.0 33.0 36.0 22.0 37.5 84-85 31.421006265627867 35.0 33.0 36.0 20.5 37.0 86-87 31.082481343103467 35.0 32.5 35.0 19.0 37.0 88-89 30.54606298452419 35.0 32.0 35.0 9.0 36.0 90-91 30.31290487637694 35.0 32.0 35.0 4.5 36.0 92-93 30.046020747048203 34.5 31.0 35.0 2.0 36.0 94-95 29.90219731417924 34.0 31.0 35.0 2.0 35.0 96-97 29.682345217752292 34.0 31.0 35.0 2.0 35.0 98-99 29.375552707542887 34.0 31.0 35.0 2.0 35.0 100-101 26.33197207982159 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.01214402108285384 1101 2 0.09293424248987492 1101 3 0.0844727204750626 1101 4 0.10033625583951533 1101 5 0.03844841861515391 1101 6 0.06160805927650159 1101 7 0.030630355653507024 1101 8 0.10190155451751792 1101 9 0.2934742122083165 1101 10-11 -0.0669581483048276 1101 12-13 0.1756220197009739 1101 14-15 0.16544209845449132 1101 16-17 0.2783727200757866 1101 18-19 0.39073416702758124 1101 20-21 0.31285976475081156 1101 22-23 0.17059830749676053 1101 24-25 0.28083373588253835 1101 26-27 0.3362992754758949 1101 28-29 0.23564074716686179 1101 30-31 0.37075440232712964 1101 32-33 0.27506027210544204 1101 34-35 0.14547796279072855 1101 36-37 0.12007660314522894 1101 38-39 0.24130029579180245 1101 40-41 0.24683155107933175 1101 42-43 0.09407009699983604 1101 44-45 0.3270894084146434 1101 46-47 -0.006452031800606051 1101 48-49 0.198980669945378 1101 50-51 0.3372035076173532 1101 52-53 0.5591562589932906 1101 54-55 0.40402850276485935 1101 56-57 0.3559132892287167 1101 58-59 0.20156218631736778 1101 60-61 0.5590195450492388 1101 62-63 0.30057414417333206 1101 64-65 0.5039088256353637 1101 66-67 0.4371546347678432 1101 68-69 0.42217188864657373 1101 70-71 0.4235799601215149 1101 72-73 0.4339377904374331 1101 74-75 0.40195852080016437 1101 76-77 0.23444648282426073 1101 78-79 0.27347658165970756 1101 80-81 0.22748440104367518 1101 82-83 0.16941687029290264 1101 84-85 0.4223450369654529 1101 86-87 0.6339107904734718 1101 88-89 0.5611128075851184 1101 90-91 0.6098032530897761 1101 92-93 0.9374408250564912 1101 94-95 0.9003880956003236 1101 96-97 0.7320067596058948 1101 98-99 0.5817581194191312 1101 100-101 0.9548752045256386 1102 1 0.07131278593267609 1102 2 0.19153702982276144 1102 3 0.16997220627243337 1102 4 0.11411334013328656 1102 5 0.07836118147908167 1102 6 0.03603977671328806 1102 7 0.08103994464602238 1102 8 0.13089194424765083 1102 9 0.3736720915657443 1102 10-11 0.33179712670492023 1102 12-13 0.2701732449217289 1102 14-15 0.19629425633242903 1102 16-17 0.432199096897655 1102 18-19 0.35714255030358544 1102 20-21 0.2309153695594972 1102 22-23 0.22753295056921274 1102 24-25 0.020797919009602595 1102 26-27 0.11717803004165717 1102 28-29 0.21779436963574028 1102 30-31 0.01835715419775852 1102 32-33 0.18505708050290792 1102 34-35 0.20977988839092632 1102 36-37 0.13114082528076665 1102 38-39 -0.036236141670528355 1102 40-41 -0.3995477531513174 1102 42-43 -0.5407839758396236 1102 44-45 -0.3387320746166651 1102 46-47 -0.11642933596033345 1102 48-49 -0.375268416901676 1102 50-51 -0.390127248792453 1102 52-53 -0.5211125448650122 1102 54-55 -0.4955424040048868 1102 56-57 -0.308693611725225 1102 58-59 -0.5052093304030834 1102 60-61 -0.668967262993597 1102 62-63 -0.5418138837709208 1102 64-65 -0.46969839447881867 1102 66-67 -0.4372824020065096 1102 68-69 -0.3341315263681395 1102 70-71 -0.3764395441162236 1102 72-73 -0.6234046685150147 1102 74-75 -0.520902218942382 1102 76-77 -0.613243151559935 1102 78-79 -0.3922562457455143 1102 80-81 -0.2892537811613636 1102 82-83 -0.3881206310369336 1102 84-85 -0.5028828079935863 1102 86-87 -0.5947675314528382 1102 88-89 -0.5471711741163645 1102 90-91 -0.4588516637512505 1102 92-93 -0.430205190759672 1102 94-95 -0.6519719171660867 1102 96-97 -0.7880737397025896 1102 98-99 -0.5818647040852483 1102 100-101 0.21421064195249429 1103 1 0.09280590650582354 1103 2 -0.0695473958061612 1103 3 0.04248706420616344 1103 4 0.05769389862876295 1103 5 0.0930237232034159 1103 6 0.1573049399470321 1103 7 0.11468807620997268 1103 8 0.19146346586289553 1103 9 0.17878484903541647 1103 10-11 0.2561559966878306 1103 12-13 0.40607652587914345 1103 14-15 0.4336914719963545 1103 16-17 0.40752678557306155 1103 18-19 0.3071344089700361 1103 20-21 0.1821935589269117 1103 22-23 0.44289157631210685 1103 24-25 0.31777748426238617 1103 26-27 0.2814172224213678 1103 28-29 0.24556038770950295 1103 30-31 0.3563242632102117 1103 32-33 0.6088501278040539 1103 34-35 0.628675923561488 1103 36-37 0.621346801019591 1103 38-39 0.7001311952992708 1103 40-41 0.5141174752130908 1103 42-43 0.3663763270179814 1103 44-45 0.22527916042363216 1103 46-47 -0.0037777036229513783 1103 48-49 -0.009824307156499401 1103 50-51 0.012127900601022645 1103 52-53 -0.04639545620588592 1103 54-55 0.3083532240989939 1103 56-57 0.30504488864114165 1103 58-59 0.3543445245183534 1103 60-61 0.6211857940771495 1103 62-63 0.5307823874983129 1103 64-65 0.634095466955678 1103 66-67 0.49706995490955563 1103 68-69 0.45995379480746124 1103 70-71 0.05049605793539058 1103 72-73 0.0024766308418193717 1103 74-75 0.31146772325415384 1103 76-77 0.2884298924846789 1103 78-79 0.17953810416899785 1103 80-81 0.1815758207274243 1103 82-83 0.2261307733431046 1103 84-85 0.15533993889235376 1103 86-87 0.4115786380497255 1103 88-89 -0.013608526554691025 1103 90-91 0.18404502272599643 1103 92-93 0.33174221622558875 1103 94-95 0.22343311417804657 1103 96-97 0.16947327649866395 1103 98-99 -0.0686350370657891 1103 100-101 0.0209557367990314 1104 1 -0.06326141665319085 1104 2 -0.008062049186044362 1104 3 -0.05421347328059767 1104 4 -0.03889678754958936 1104 5 0.034928074099880746 1104 6 0.1396110327713842 1104 7 -0.041434172603239006 1104 8 -0.057428212900497044 1104 9 -0.14208297630100475 1104 10-11 -0.10400721097037291 1104 12-13 -0.19386462520564152 1104 14-15 -0.10791863432401527 1104 16-17 -0.06203263221500066 1104 18-19 0.09212008906733615 1104 20-21 -0.11877548654368297 1104 22-23 -0.06978055676490413 1104 24-25 0.04495783657122132 1104 26-27 0.17349615843298238 1104 28-29 0.26457251058603504 1104 30-31 -0.16603832457275303 1104 32-33 -0.14230134930371463 1104 34-35 -0.16124914215313169 1104 36-37 -0.389348008506083 1104 38-39 -0.12728062940942664 1104 40-41 -0.009555481748741101 1104 42-43 0.044033355540086916 1104 44-45 0.07829418651331821 1104 46-47 -0.019435438332038757 1104 48-49 -0.11930290576766112 1104 50-51 0.29059312089211886 1104 52-53 0.13791542299690462 1104 54-55 0.15440446188784307 1104 56-57 0.03683507264207719 1104 58-59 0.016406590507607177 1104 60-61 -0.046851398437269154 1104 62-63 -0.0795632517970759 1104 64-65 0.030768541615337597 1104 66-67 0.007792227262335416 1104 68-69 0.2304626417610507 1104 70-71 0.15336788499804754 1104 72-73 -0.17479984136187454 1104 74-75 -0.0631840873308036 1104 76-77 -0.08531956806692875 1104 78-79 -0.08450913189361131 1104 80-81 -0.19829785026790248 1104 82-83 -0.0014693678942911959 1104 84-85 -0.03980698723264098 1104 86-87 0.07833292421238625 1104 88-89 -0.19855105078082858 1104 90-91 9.032753055429055E-4 1104 92-93 0.024727425531565927 1104 94-95 0.07380778560493795 1104 96-97 -0.24317139375124697 1104 98-99 -0.3556019639754382 1104 100-101 -0.006114764201399225 1105 1 -0.1020816508827096 1105 2 -0.03190052007663979 1105 3 0.02659725018391157 1105 4 0.010226775447691239 1105 5 -0.05200001035817081 1105 6 -0.07851328431567595 1105 7 0.06542342754450203 1105 8 0.03299330789852917 1105 9 -0.06523507159332809 1105 10-11 -0.1789891282051812 1105 12-13 -0.04982962928387735 1105 14-15 0.08316233029532327 1105 16-17 0.13611600042015937 1105 18-19 -0.30324610616497694 1105 20-21 -0.21928103448993852 1105 22-23 -0.19028872094101956 1105 24-25 -0.157426779997202 1105 26-27 -0.019139154514277834 1105 28-29 -0.2994827995910896 1105 30-31 -0.20734782456505485 1105 32-33 0.10391665959146223 1105 34-35 0.10997309887858364 1105 36-37 0.03855946929443377 1105 38-39 0.08701937520744707 1105 40-41 0.17676240556870226 1105 42-43 0.3863708799710608 1105 44-45 0.6376595199287323 1105 46-47 0.9783715454627213 1105 48-49 0.8778692575466351 1105 50-51 0.6784145708990508 1105 52-53 0.7917766704768638 1105 54-55 0.6309545780792263 1105 56-57 0.6669155601842078 1105 58-59 0.7184757205342294 1105 60-61 0.8057317438086855 1105 62-63 0.9877584983068104 1105 64-65 0.6885425916930572 1105 66-67 0.577487821223464 1105 68-69 0.409393527124017 1105 70-71 0.5878005478583503 1105 72-73 0.9358359024938352 1105 74-75 0.8943406514309515 1105 76-77 0.782399233392006 1105 78-79 0.6532599935161301 1105 80-81 0.7854276610511377 1105 82-83 0.7928026276735061 1105 84-85 1.0587209987320563 1105 86-87 0.8888255449052238 1105 88-89 1.0563082114423494 1105 90-91 1.111023467983177 1105 92-93 1.1645956906633756 1105 94-95 1.0638968913160127 1105 96-97 1.1698351160442257 1105 98-99 1.1173816153348 1105 100-101 1.3192788244964895 1106 1 0.06980620617618172 1106 2 0.052485867604090686 1106 3 -0.008726600762372527 1106 4 0.11208406944086846 1106 5 0.1847902799909491 1106 6 0.11714911135847927 1106 7 -0.05951779732913565 1106 8 0.055562956423273135 1106 9 -0.18027268767420424 1106 10-11 0.06315704553409063 1106 12-13 0.04939013091360778 1106 14-15 0.09413123825347469 1106 16-17 0.07440946462610754 1106 18-19 0.2103970700428519 1106 20-21 0.12633874921759514 1106 22-23 0.1632803986075828 1106 24-25 0.2550864444062242 1106 26-27 0.14603364435723876 1106 28-29 -0.00554839263212159 1106 30-31 0.07401605224228547 1106 32-33 0.11986675950917913 1106 34-35 0.15178425439756893 1106 36-37 0.34100892268575933 1106 38-39 0.4239624808851232 1106 40-41 0.19399086448488845 1106 42-43 0.09699889219628943 1106 44-45 0.3113032838878169 1106 46-47 0.17870656368300786 1106 48-49 0.10862040366159675 1106 50-51 0.36874312385543817 1106 52-53 0.5752931862873751 1106 54-55 0.5818773475631787 1106 56-57 0.5776287963426654 1106 58-59 0.5749468619471827 1106 60-61 0.5012839361208989 1106 62-63 0.5940165798866772 1106 64-65 0.8631634921279883 1106 66-67 0.7705641113374853 1106 68-69 0.7208355748494171 1106 70-71 0.7047850900001151 1106 72-73 0.3911153782197019 1106 74-75 0.6226379360201122 1106 76-77 0.6885647441478469 1106 78-79 0.23262845478332395 1106 80-81 0.5134457637900525 1106 82-83 0.7524755440952795 1106 84-85 0.7858501276846823 1106 86-87 0.8870843317866175 1106 88-89 0.7308850305325123 1106 90-91 0.8308497874847625 1106 92-93 0.8968456025006866 1106 94-95 0.821155619422278 1106 96-97 0.760943908803128 1106 98-99 0.7917747621550646 1106 100-101 0.9943038039251952 1107 1 0.17629290089556093 1107 2 0.13121371683066485 1107 3 0.10456089997845908 1107 4 0.17558789211614823 1107 5 0.11849353370990912 1107 6 0.06440211912236293 1107 7 0.192549114304839 1107 8 0.11527373696022636 1107 9 0.22560504598311582 1107 10-11 0.21075602022739304 1107 12-13 0.32702673969686913 1107 14-15 0.1998808085729209 1107 16-17 0.12181137892705607 1107 18-19 0.24114515870520847 1107 20-21 0.17788189507191987 1107 22-23 0.2519464627337058 1107 24-25 0.33325867222582417 1107 26-27 0.5147217851739327 1107 28-29 0.3662438197782336 1107 30-31 0.3548530403297576 1107 32-33 0.5095092356544981 1107 34-35 0.43624389405856334 1107 36-37 0.6677550555607681 1107 38-39 0.6582346909248358 1107 40-41 0.7258205628195071 1107 42-43 0.479966503172399 1107 44-45 0.39166466005092104 1107 46-47 0.44574562016377683 1107 48-49 0.3890536401933389 1107 50-51 0.29894966685312596 1107 52-53 0.3858262865066564 1107 54-55 0.5137359382109636 1107 56-57 0.3320996965975169 1107 58-59 0.2879943342401887 1107 60-61 0.20523773805298617 1107 62-63 -0.12413149692242342 1107 64-65 0.21739806807773476 1107 66-67 0.18188693478322904 1107 68-69 0.27920915986690176 1107 70-71 0.32746259251893406 1107 72-73 0.4213004371896645 1107 74-75 0.25157914386696945 1107 76-77 0.15562750714197904 1107 78-79 -0.024620552506426918 1107 80-81 8.664951359946826E-4 1107 82-83 0.03613508893021944 1107 84-85 -0.09412453597915871 1107 86-87 0.11175910805639688 1107 88-89 0.4407247152358842 1107 90-91 0.17081970984593298 1107 92-93 0.45091126954270777 1107 94-95 0.6086267271091153 1107 96-97 0.23872275666657927 1107 98-99 0.12072115580172138 1107 100-101 0.588592756689966 1108 1 -0.2617363470153009 1108 2 -0.11873753665123132 1108 3 -0.15888617523045667 1108 4 -0.03382018587827673 1108 5 -0.0885607838388367 1108 6 -0.05822322906705324 1108 7 -0.21228375477593886 1108 8 -0.041564703151195204 1108 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sequence quality scores pass #Quality Count 3 2.0 4 2.0 5 10.0 6 31.0 7 52.0 8 94.0 9 217.0 10 360.0 11 667.0 12 985.0 13 1449.0 14 1768.0 15 2343.0 16 3106.0 17 4550.0 18 4052.0 19 2144.0 20 1718.0 21 1622.0 22 1709.0 23 1929.0 24 2133.0 25 2511.0 26 2804.0 27 3357.0 28 4221.0 29 5046.0 30 6544.0 31 7970.0 32 10074.0 33 13052.0 34 17619.0 35 29416.0 36 40487.0 37 80856.0 38 132258.0 39 27157.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.494161615770416 23.20394647663678 15.043763676148798 20.258128231444008 2 13.804616264837735 25.15915032916416 39.22207657080229 21.81415683519582 3 18.145943754163408 29.890858362054818 32.31915119568261 19.644046688099166 4 10.10199747057858 19.718867359843987 42.839420357015285 27.33971481256215 5 9.115330031569496 42.64030082737181 36.863420897654976 11.380948243403713 6 24.73740357787625 43.039505314681264 18.030575105473012 14.192516001969475 7 23.044525540398336 36.982651258435425 24.46563558953862 15.507187611627614 8 23.214199515355133 35.034417509002616 22.33397051582819 19.41741245981406 9 24.288479547407345 18.35520027804327 24.56845367393634 32.78786650061304 10-11 21.769998008797494 28.86181658007953 29.850056417404318 19.518128993718662 12-13 24.165807835915455 28.173912623725933 29.22273202308344 18.437547517275167 14-15 21.142753014549 30.10928645214856 29.30508490939458 19.44287562390786 16-17 17.17762314726987 33.02632499921558 30.40118651963091 19.394865333883633 18-19 16.788069240497773 32.91976810418899 32.94149023469555 17.35067242061768 20-21 18.448967474729923 30.81429026558925 32.01540340409921 18.721338855581624 22-23 18.139065079503 30.42908448460625 31.976665604695842 19.45518483119491 24-25 19.153858407592274 31.15810154568011 31.843327315382464 17.844712731345158 26-27 17.975168711804514 30.217153090877495 34.20468917444656 17.602989022871434 28-29 17.157014779835126 31.336581268577373 33.89840326611789 17.608000685469612 30-31 18.00570330045629 30.756550166959716 32.80508011290749 18.432666419676508 32-33 17.29729990659371 31.59377194866757 33.36740530847004 17.741522836268672 34-35 18.392635608397416 30.429283786849716 32.941846600920066 18.236234003832806 36-37 18.654626155648906 29.620091546822714 33.05798488379249 18.667297413735884 38-39 17.541606981018457 31.18493158124026 33.288239745030154 17.985221692711132 40-41 16.853558343510603 31.43726377069043 33.918908481808835 17.790269403990134 42-43 17.217787775731523 31.185328753798515 31.90906743067339 19.687816039796576 44-45 17.741559812943127 30.410016593754712 32.101734801629206 19.746688791672952 46-47 18.75477505205033 30.64795872182493 32.217615642255815 18.379650583868923 48-49 18.440205085621315 30.90864323888516 31.887241530803877 18.763910144689643 50-51 17.0650082910734 30.882619158534496 32.76530831448633 19.287064235905778 52-53 17.59566785260851 32.10732371384849 31.532375246803024 18.764633186739978 54-55 18.060853709015827 30.441577374092343 31.42378563506842 20.073783281823417 56-57 19.085346721054282 30.725422992445274 30.474041176896527 19.71518910960392 58-59 19.111217309347015 30.015845259515906 31.373855201118943 19.49908223001814 60-61 18.648312049411203 31.527639331046853 31.386564458786587 18.43748416075536 62-63 17.761220687191667 31.527379538718492 31.967976752493215 18.743423021596627 64-65 17.7330110385559 31.774676850409282 31.471167851940706 19.021144259094108 66-67 18.20519689904519 31.304727701026252 29.859602629825932 20.630472770102624 68-69 17.719103889709505 30.66682113515027 31.15098328844093 20.463091686699297 70-71 19.310946558473844 30.250143608111564 31.077881454520877 19.36102837889371 72-73 18.759782725441664 29.3857685405652 31.774895640871847 20.079553093121287 74-75 17.70148482829863 31.92875623907859 30.190261727536903 20.179497205085877 76-77 17.70417664272413 30.241239207719655 31.33667077801156 20.717913371544658 78-79 17.600991673231107 30.42254848421877 31.399965204448034 20.576494638102098 80-81 18.802993842878763 30.668666745511285 30.08167659062988 20.446662820980073 82-83 18.740526737529084 29.869136231548254 31.23207924233209 20.15825778859057 84-85 17.409626559021053 29.942858522045025 31.196229989923307 21.451284929010615 86-87 17.778902814915416 30.59542523383118 30.910422207105377 20.71524974414803 88-89 18.042454834378557 30.02661014438917 30.42309158405625 21.50784343717602 90-91 18.013349408791147 28.511664390924146 31.39181023212574 22.083175968158972 92-93 19.1131885248977 30.10102379330002 30.64928997713742 20.136497704664862 94-95 18.08808231378389 28.607201732007137 31.5335841879527 21.77113176625627 96-97 17.35475353175015 28.839790206920856 31.84404061663525 21.961415644693748 98-99 17.78210083861212 29.639972436464486 30.511047740188335 22.066878984735062 100-101 17.145743100466667 28.761360441106724 31.23299177088324 22.85990468754337 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2784.0 1 1617.0 2 725.5 3 934.5 4 606.5 5 564.5 6 1238.0 7 1992.0 8 2281.0 9 2029.0 10 1371.0 11 909.5 12 841.0 13 771.5 14 692.0 15 647.0 16 632.0 17 660.5 18 714.0 19 740.0 20 804.0 21 959.5 22 1151.0 23 1331.5 24 1465.5 25 1793.0 26 2852.0 27 4133.5 28 4385.0 29 4863.5 30 6701.0 31 8349.0 32 10819.0 33 14128.5 34 16945.5 35 19754.5 36 23659.5 37 26794.5 38 25568.5 39 22668.0 40 20024.0 41 16715.0 42 12741.5 43 12161.0 44 13399.5 45 12877.0 46 11440.0 47 13658.5 48 22260.0 49 20174.5 50 11193.0 51 7420.0 52 4765.5 53 3211.5 54 2104.5 55 1757.0 56 1544.0 57 1392.5 58 1280.0 59 1072.0 60 871.5 61 666.0 62 425.0 63 208.5 64 99.0 65 58.0 66 42.5 67 39.0 68 34.0 69 53.5 70 54.5 71 28.5 72 18.5 73 15.5 74 12.5 75 8.5 76 5.5 77 2.0 78 2.0 79 1.5 80 0.0 81 0.5 82 0.5 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28866297873162067 2 0.022446201523445418 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.6203550844266804E-4 12-13 2.413570056284454E-4 14-15 0.0 16-17 7.240710168853361E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0019308560450275632 26-27 0.0019308560450275632 28-29 0.002292891553470231 30-31 6.033925140711134E-4 32-33 0.0012067850281422268 34-35 0.0014481420337706722 36-37 1.206785028142227E-4 38-39 1.206785028142227E-4 40-41 4.827140112568908E-4 42-43 0.004103069095683571 44-45 0.002775605564727122 46-47 0.01484345584614939 48-49 0.013998706326449832 50-51 0.005551211129454244 52-53 0.006275282146339579 54-55 6.033925140711134E-4 56-57 0.0033789980787982347 58-59 0.0010861065253280043 60-61 0.0012067850281422268 62-63 0.0 64-65 8.447495196995587E-4 66-67 0.0 68-69 0.0 70-71 4.827140112568908E-4 72-73 0.002292891553470231 74-75 0.0 76-77 0.20129174269412345 78-79 0.11536864869039688 80-81 0.0016894990393991173 82-83 0.0 84-85 3.6203550844266804E-4 86-87 0.12321275137332137 88-89 0.5915660207953196 90-91 0.30700611115938253 92-93 0.290473156273834 94-95 0.0016894990393991173 96-97 0.002654927061912899 98-99 0.0020515345478417856 100-101 6.033925140711134E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 414324.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.48891205916143 #Duplication Level Percentage of deduplicated Percentage of total 1 69.15043600856352 12.785163302150007 2 10.378048143700068 3.837576389492282 3 4.643360660017754 2.5755206070611405 4 2.720484569996345 2.011951998918721 5 1.822359145736515 1.6846718992865488 6 1.3824343376324997 1.5335824137631417 7 1.0652185264477052 1.3786312161496799 8 0.8028301394183072 1.1874764676919514 9 0.693175291107514 1.1534451298983406 >10 5.575426870659496 21.470636506695243 >50 1.007780272570623 13.210917060078586 >100 0.693175291107514 23.624989138934748 >500 0.04438410526865438 5.414603064268544 >1k 0.019581222912641638 6.813990982902269 >5k 0.0013054148608427757 1.316843822708798 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5456 1.316843822708798 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3575 0.8628512951216922 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2914 0.7033143144012899 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2908 0.7018661723675191 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2709 0.6538361282474585 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2233 0.5389501935683185 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2085 0.5032293567353086 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1522 0.36734536256649386 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1473 0.35551886929070003 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1413 0.3410374489529933 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1379 0.33283131076162614 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1264 0.30507525511435496 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1261 0.30435118409746964 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1222 0.29493826087796027 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1213 0.2927660478273042 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1061 0.2560797829717805 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 997 0.24063293461156002 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 990 0.2389434355721609 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 943 0.227599656307624 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 861 0.20780838184609146 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 815 0.19670595958718298 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 802 0.19356831851401318 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 798 0.1926028904914994 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 758 0.18294861026636158 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 750 0.18101775422133404 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 749 0.18077639721570557 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 735 0.17739739913690736 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 731 0.17643197111439357 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 691 0.16677769088925576 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 668 0.1612264797598015 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 634 0.15302034156843436 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 634 0.15302034156843436 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 609 0.14698641642772323 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 600 0.1448142033770672 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 586 0.14143520529826897 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 579 0.13974570625886987 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 573 0.1382975642250992 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 564 0.1361253511744432 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 556 0.13419449512941561 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 549 0.1325049960900165 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 548 0.13226363908438807 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 548 0.13226363908438807 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 543 0.13105685405624584 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 542 0.13081549705061737 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 530 0.12791921298307604 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 524 0.12647107094930538 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 513 0.12381614388739248 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 507 0.12236800185362182 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 506 0.12212664484799336 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 501 0.12091985981985112 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 483 0.11657543371853911 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 479 0.11561000569602534 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 475 0.11464457767351155 No Hit ACCTAAAACCTGGTGAACTACGACTGCTAGAAGTTGATAACCGAGTCGTT 475 0.11464457767351155 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 475 0.11464457767351155 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 475 0.11464457767351155 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 468 0.11295507863411244 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 465 0.1122310076172271 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 460 0.11102422258908488 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 453 0.10933472354968576 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 441 0.1064384394821444 No Hit CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC 441 0.1064384394821444 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 439 0.10595572547088751 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 435 0.10499029744837374 No Hit GCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGT 433 0.10450758343711684 No Hit TATATAATCCCAACAAACGACCTAAAACCTGGTGAACTACGACTGCTAGA 428 0.10330079840897462 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 424 0.10233537038646084 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.6203550844266804E-4 0.0 16-17 0.0 0.0 0.0 0.0012067850281422268 0.0 18-19 0.0 0.0 0.0 0.0028962840675413443 0.0 20-21 0.0 0.0 0.0 0.0049478186153831295 0.0 22-23 0.0 0.0 0.0 0.008447495196995588 0.0 24-25 0.0 0.0 0.0 0.014240063332078277 0.0 26-27 0.0 0.0 0.0 0.019911952964346745 0.0 28-29 0.0 0.0 0.0 0.03282455276546857 0.0 30-31 0.0 0.0 0.0 0.06794199708440737 0.0 32-33 0.0 0.0 0.0 0.13129821106187428 0.0 34-35 0.0 0.0 0.0 0.21927283961344263 0.0 36-37 0.0 0.0 0.0 0.35093308618375957 0.0 38-39 0.0 0.0 0.0 0.5306233768741372 0.0 40-41 0.0 0.0 0.0 0.7315530840598179 0.0 42-43 0.0 0.0 0.0 0.907743698168583 0.0 44-45 0.0 0.0 0.0 1.102880837219181 0.0 46-47 0.0 0.0 0.0 1.3506338034967804 0.0 48-49 0.0 0.0 0.0 1.6079203714967032 0.0 50-51 0.0 0.0 0.0 1.8478292350913779 0.0 52-53 0.0 0.0 0.0 2.044052480667304 0.0 54-55 0.0 0.0 0.0 2.2735829930199554 0.0 56-57 0.0 0.0 0.0 2.5397997702281305 0.0 58-59 0.0 0.0 0.0 2.8138606501192305 0.0 60-61 0.0 0.0 0.0 3.110247053030961 0.0 62-63 0.0 0.0 0.0 3.3817736843629627 0.0 64-65 0.0 0.0 0.0 3.665609522982014 0.0 66-67 0.0 0.0 0.0 3.998320155240826 0.0 68-69 0.0 0.0 0.0 4.376043869049343 0.0 70-71 0.0 0.0 0.0 4.794315559803438 0.0 72-73 0.0 0.0 0.0 5.165522634459988 0.0 74-75 0.0 0.0 0.0 5.558572518125912 0.0 76-77 0.0 0.0 0.0 5.975999459360308 0.0 78-79 0.0 0.0 0.0 6.4922620943995515 0.0 80-81 0.0 0.0 0.0 7.054985953022273 0.0 82-83 0.0 0.0 0.0 7.5707658740502595 0.0 84-85 0.0 0.0 0.0 8.113698458211449 0.0 86-87 0.0 0.0 0.0 8.684628455025535 0.0 88-89 0.0 0.0 0.0 9.299123391355558 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAAAGT 25 5.3022588E-5 75.91041 4 AGGACCG 30 1.3078579E-4 63.25867 5 TCCGACC 25 0.005042503 56.932804 8 GGTCACC 25 0.005042503 56.932804 8 GACCGCG 45 1.5248814E-5 52.715557 7 ATAGGAC 50 2.8435952E-5 47.444004 3 GGACCGC 40 5.41557E-4 47.444 6 GGTACTC 45 9.6739334E-4 42.172443 3 GGGATGC 85 3.6669553E-7 39.07153 7 GTTATGG 50 0.0016240068 37.955204 3 TACTCCC 80 1.08467975E-5 35.583 5 ACCATAC 55 0.002542428 34.642696 1 TGGTACT 55 0.0025911492 34.508896 2 CTCCCGC 55 0.0025926835 34.50473 7 GGTATGG 55 0.0025926835 34.50473 8 TGTTATG 70 2.0638708E-4 33.892662 2 GGACTGG 60 0.003970924 31.629335 6 TCTAGAC 120 1.5174919E-7 31.629335 3 CCGCGGT 75 3.0964243E-4 31.629335 9 ACTCCCG 60 0.003970924 31.629335 6 >>END_MODULE