##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936307_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 66699 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07665782095684 33.0 31.0 34.0 30.0 34.0 2 32.12049655916881 33.0 31.0 34.0 30.0 34.0 3 32.16286600998516 34.0 31.0 34.0 30.0 34.0 4 35.75540862681599 37.0 35.0 37.0 33.0 37.0 5 35.76674312958215 37.0 35.0 37.0 35.0 37.0 6 35.913956730985475 37.0 35.0 37.0 35.0 37.0 7 35.8489182746368 37.0 35.0 37.0 35.0 37.0 8 35.90927899968515 37.0 35.0 37.0 35.0 37.0 9 37.489887404608766 39.0 37.0 39.0 34.0 39.0 10-11 37.50149177648841 39.0 37.0 39.0 35.0 39.0 12-13 37.44197064423754 39.0 37.0 39.0 34.5 39.0 14-15 38.37864135894091 40.0 38.0 41.0 33.5 41.0 16-17 38.25520622498088 40.0 37.5 41.0 33.5 41.0 18-19 38.26569363858529 40.0 37.0 41.0 34.0 41.0 20-21 38.262207829202836 40.0 37.0 41.0 34.0 41.0 22-23 38.04626756023329 40.0 35.5 41.0 34.0 41.0 24-25 37.92244261533156 40.0 35.5 41.0 33.5 41.0 26-27 37.557227244786276 39.5 35.0 41.0 33.0 41.0 28-29 37.22959114829308 39.0 35.0 41.0 33.0 41.0 30-31 36.71225205775199 38.5 35.0 41.0 33.0 41.0 32-33 36.049895800536746 37.0 35.0 40.0 32.0 41.0 34-35 35.67366827088862 37.0 35.0 40.0 31.0 41.0 36-37 35.33646681359541 36.5 35.0 40.0 30.5 41.0 38-39 34.97251832861062 36.0 35.0 40.0 30.0 41.0 40-41 34.59889953372614 35.0 35.0 40.0 28.0 41.0 42-43 34.10473920148728 35.5 35.0 40.0 23.5 41.0 44-45 33.440628795034414 35.0 34.5 40.0 17.5 41.0 46-47 32.69948574941154 35.0 33.0 40.0 2.0 41.0 48-49 32.06756473110541 35.0 33.0 40.0 2.0 41.0 50-51 31.585630968980045 35.0 33.0 40.0 2.0 41.0 52-53 31.275498883041724 35.0 33.0 40.0 2.0 41.0 54-55 30.9460486663968 35.0 32.5 40.0 2.0 41.0 56-57 30.43627340739741 35.0 31.0 40.0 2.0 41.0 58-59 29.994475179537922 35.0 31.0 40.0 2.0 41.0 60-61 29.394930958485133 35.0 28.0 39.0 2.0 41.0 62-63 28.935823625541612 35.0 27.0 39.0 2.0 41.0 64-65 28.449137168473293 35.0 26.0 38.5 2.0 40.5 66-67 27.8498178383484 35.0 23.5 37.5 2.0 40.0 68-69 27.314270079011678 35.0 21.0 37.0 2.0 39.5 70-71 26.732394788527564 35.0 14.5 36.0 2.0 39.0 72-73 26.239486349120675 34.5 4.5 35.5 2.0 38.5 74-75 25.823235730670625 34.0 2.0 35.0 2.0 37.0 76-77 25.13029430726098 33.0 2.0 35.0 2.0 36.5 78-79 25.11406467863086 34.0 2.0 35.0 2.0 36.0 80-81 24.917832351309613 34.0 2.0 35.0 2.0 36.0 82-83 24.603022534070973 34.0 2.0 35.0 2.0 35.5 84-85 24.295978950209147 33.5 2.0 35.0 2.0 35.0 86-87 23.936700700160422 33.0 2.0 35.0 2.0 35.0 88-89 23.453245176089595 33.0 2.0 35.0 2.0 35.0 90-91 23.147610908709275 33.0 2.0 35.0 2.0 35.0 92-93 22.81645152101231 33.0 2.0 35.0 2.0 35.0 94-95 22.62434219403589 32.5 2.0 35.0 2.0 35.0 96-97 22.330634642198532 32.0 2.0 35.0 2.0 35.0 98-99 21.96414488972848 32.0 2.0 35.0 2.0 35.0 100-101 19.98512721330155 28.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5728861129794041 1101 2 0.4396464294555571 1101 3 0.4725108450559645 1101 4 0.0460531632575254 1101 5 -0.2968594058487284 1101 6 0.315808235222633 1101 7 -0.04648938103776601 1101 8 -0.43119014661025545 1101 9 -0.0029704782857109535 1101 10-11 -0.23064259078726934 1101 12-13 0.18437841102890218 1101 14-15 0.28884132098163917 1101 16-17 0.2890113196492621 1101 18-19 0.6579422077720452 1101 20-21 0.35951018237963694 1101 22-23 0.6395138538538347 1101 24-25 0.9071211408790205 1101 26-27 0.6755342692821671 1101 28-29 1.1073388590988742 1101 30-31 0.6601237297005937 1101 32-33 0.08502208776451425 1101 34-35 0.6457687634068847 1101 36-37 1.256705058871141 1101 38-39 0.18896307811955637 1101 40-41 -0.1875348109330659 1101 42-43 0.79688095906765 1101 44-45 0.9627662454537642 1101 46-47 1.0478849490011584 1101 48-49 0.567177494760891 1101 50-51 0.11727327088477679 1101 52-53 -0.39377565810944404 1101 54-55 0.26269414098237576 1101 56-57 0.8366613988263545 1101 58-59 -0.25189732112880137 1101 60-61 -0.03856845076854398 1101 62-63 0.2722068158695343 1101 64-65 0.9967664291464722 1101 66-67 0.22901675146458444 1101 68-69 -0.11463545141700493 1101 70-71 -0.4100440811543393 1101 72-73 0.0815842594823053 1101 74-75 0.039859596473835524 1101 76-77 0.055175239351694216 1101 78-79 -0.632423453703268 1101 80-81 -0.1829405573255265 1101 82-83 -1.2579319677684673 1101 84-85 -0.9678696437464112 1101 86-87 -0.5815614959028217 1101 88-89 -0.40346650848379895 1101 90-91 -0.46567496647457673 1101 92-93 -0.22464920911848552 1101 94-95 -0.2734686929989181 1101 96-97 -1.1377215949428852 1101 98-99 -0.3746809530914419 1101 100-101 -0.18515923815835222 1102 1 0.14478153781600867 1102 2 0.016988477385837086 1102 3 0.15442805638493695 1102 4 -0.2611363792261301 1102 5 -0.7521970964805362 1102 6 0.20796509796772966 1102 7 0.5352099653674642 1102 8 -0.07171302242725375 1102 9 -0.03347156761032721 1102 10-11 -0.266045640896202 1102 12-13 0.42511915176963555 1102 14-15 0.5246801009380704 1102 16-17 0.7356344133311836 1102 18-19 0.6595761947001506 1102 20-21 0.995131097412326 1102 22-23 0.9581413048342213 1102 24-25 0.7110427095064722 1102 26-27 0.6689983215697524 1102 28-29 0.6770556346980001 1102 30-31 0.9689472591123618 1102 32-33 1.0539763361305248 1102 34-35 1.356553077132368 1102 36-37 1.9097551678036027 1102 38-39 1.272296411452892 1102 40-41 1.0581078905919838 1102 42-43 0.44829708107199906 1102 44-45 -0.821002817727063 1102 46-47 -0.16562267627116967 1102 48-49 -0.24328002157898254 1102 50-51 -0.08316245896272179 1102 52-53 0.6612352351367434 1102 54-55 0.49798825862943374 1102 56-57 0.49679211778060406 1102 58-59 -0.13806289847085296 1102 60-61 0.2364860154623898 1102 62-63 0.101727513037293 1102 64-65 0.28652677773034796 1102 66-67 0.6663805858872429 1102 68-69 0.7502882958596828 1102 70-71 1.0850539580613443 1102 72-73 0.1763555013123721 1102 74-75 0.11229968361980269 1102 76-77 0.3411229517699965 1102 78-79 0.35123667701568806 1102 80-81 0.7457086801472386 1102 82-83 0.9163599712293511 1102 84-85 0.6535900512426984 1102 86-87 0.4930572404806206 1102 88-89 0.287709962104433 1102 90-91 0.875283639189913 1102 92-93 -0.23772110454332207 1102 94-95 -0.225538409774515 1102 96-97 0.6612980129002572 1102 98-99 0.42542797937043275 1102 100-101 0.11004773351920605 1103 1 -0.26632957329510276 1103 2 -0.018196707799347678 1103 3 0.38035398231086504 1103 4 -0.15002526811502293 1103 5 -0.05960450388794669 1103 6 0.3246317646343968 1103 7 0.5740988542563557 1103 8 0.38939808868386194 1103 9 0.1646765805378223 1103 10-11 0.49043584058527756 1103 12-13 0.4093784110288965 1103 14-15 0.15060602686398994 1103 16-17 0.3198936725904389 1103 18-19 0.4540206391445949 1103 20-21 0.39235331963454456 1103 22-23 0.008141304834225593 1103 24-25 -0.08895729049353207 1103 26-27 -0.20044612287469477 1103 28-29 0.2955741532165206 1103 30-31 0.6022805924457018 1103 32-33 0.1511985583527533 1103 34-35 -0.04344692286762353 1103 36-37 0.4032736863221231 1103 38-39 0.5306297447862249 1103 40-41 1.025700483184579 1103 42-43 0.914037821812741 1103 44-45 0.7632564415321994 1103 46-47 1.6214143607658684 1103 48-49 1.2039422006432403 1103 50-51 1.3260968002965363 1103 52-53 1.371420420321929 1103 54-55 1.422988258629431 1103 56-57 1.2440143400028276 1103 58-59 1.5221222867143354 1103 60-61 0.8790786080549857 1103 62-63 0.6035793648891428 1103 64-65 0.6457860369896089 1103 66-67 1.0598991044057584 1103 68-69 1.1706586662300538 1103 70-71 0.3433872913946807 1103 72-73 0.8457999457568164 1103 74-75 0.4271144984346158 1103 76-77 0.4022340628811101 1103 78-79 -0.5015411007620934 1103 80-81 0.08274571718428092 1103 82-83 0.5876562675256487 1103 84-85 1.035071532724178 1103 86-87 0.1449090923324725 1103 88-89 -0.5456233712288956 1103 90-91 -0.13675339784712293 1103 92-93 -0.15160999343221349 1103 94-95 0.11427640504030023 1103 96-97 -0.42203532043307845 1103 98-99 -1.2477201687777182 1103 100-101 0.2118995853710537 1104 1 0.4985827074066478 1104 2 0.4107506606217086 1104 3 0.06281012266174457 1104 4 0.06050104767444964 1104 5 0.1737288294453876 1104 6 0.4272633435817639 1104 7 0.2662041174142473 1104 8 0.05694194833298383 1104 9 0.6234485103623797 1104 10-11 0.6321025072519504 1104 12-13 0.7545538496253883 1104 14-15 0.6918340970394325 1104 16-17 0.4510340234676349 1104 18-19 0.9943715163375728 1104 20-21 0.3971778810380542 1104 22-23 0.7695448136061529 1104 24-25 0.9303409551205064 1104 26-27 0.9526240525639054 1104 28-29 1.3183811707603823 1104 30-31 1.5785963819193753 1104 32-33 0.4735669794053834 1104 34-35 0.42234255081658745 1104 36-37 0.8186245635151082 1104 38-39 0.7415946570669263 1104 40-41 0.9182443428337024 1104 42-43 1.0662308042688835 1104 44-45 0.6180810029357033 1104 46-47 1.204747694099197 1104 48-49 -0.27544376426904194 1104 50-51 -0.1309207435631059 1104 52-53 0.06133270102368371 1104 54-55 0.5769356270504886 1104 56-57 0.5084880242133529 1104 58-59 0.21817491829328262 1104 60-61 0.6843417659497248 1104 62-63 0.5632284876961613 1104 64-65 -0.12965255950161847 1104 66-67 0.3910394552829537 1104 68-69 0.4276762100897038 1104 70-71 0.34382588788590596 1104 72-73 0.38395784049365744 1104 74-75 0.010447831767951499 1104 76-77 0.3513568698986482 1104 78-79 -0.5054884691831454 1104 80-81 -0.9233946336929151 1104 82-83 -0.7171682938778616 1104 84-85 -0.7201916251705605 1104 86-87 -0.4840382760885831 1104 88-89 0.011394172630751598 1104 90-91 0.37333432145112155 1104 92-93 0.32953035744498393 1104 94-95 0.45769745767187686 1104 96-97 0.13936818833885312 1104 98-99 0.5535956206959654 1104 100-101 0.03207502396754691 1105 1 0.568758146003141 1105 2 0.6037331167620579 1105 3 0.39614345599507317 1105 4 0.07804490732357294 1105 5 0.4719744434804767 1105 6 0.550070361125627 1105 7 0.42409885425635707 1105 8 0.3376437027189496 1105 9 0.16730815948518796 1105 10-11 0.2022779458484365 1105 12-13 0.087887182958724 1105 14-15 0.4199042724780213 1105 16-17 0.5738410410114909 1105 18-19 0.1434943233551209 1105 20-21 -0.12036597861106912 1105 22-23 0.18182551536054348 1105 24-25 -0.29772922031808235 1105 26-27 0.02279949116039859 1105 28-29 -0.19039075906417935 1105 30-31 -0.6231580040455356 1105 32-33 0.13146171624748604 1105 34-35 0.4486583402902653 1105 36-37 -0.16383157683576854 1105 38-39 -0.2408614832839504 1105 40-41 -0.3449135519031401 1105 42-43 -0.8285060378363767 1105 44-45 -0.4170067163625433 1105 46-47 -0.5233224813393917 1105 48-49 -0.3807069221637782 1105 50-51 -0.2624996909315307 1105 52-53 0.08764849049736512 1105 54-55 1.0330759779276804 1105 56-57 0.2190143400028255 1105 58-59 -0.07129876591724482 1105 60-61 -0.7893424445765937 1105 62-63 -0.4981750210757703 1105 64-65 0.08964568611241575 1105 66-67 -0.5826447552433613 1105 68-69 -0.5986395793839812 1105 70-71 -0.9456477963246215 1105 72-73 -1.2563930366993254 1105 74-75 -1.1913065541969594 1105 76-77 -1.2100466388732762 1105 78-79 -1.1370674165515666 1105 80-81 -1.4497104231666 1105 82-83 -1.0855893465094404 1105 84-85 -0.8254547830652967 1105 86-87 -1.2647400304745453 1105 88-89 -0.9272023185973204 1105 90-91 -0.7582446259172961 1105 92-93 -1.1353819232567695 1105 94-95 -0.770372717766719 1105 96-97 -1.3606318116611469 1105 98-99 -0.7797377126373668 1105 100-101 -0.8275740988394702 1106 1 0.10033709337156438 1106 2 0.315136625533988 1106 3 0.2803539823108636 1106 4 0.3666413985516428 1106 5 0.04039549611205473 1106 6 -0.19203490203226892 1106 7 0.47409885425635423 1106 8 0.2727314220171948 1106 9 0.681343247204488 1106 10-11 0.13210250725195039 1106 12-13 0.37604507769556506 1106 14-15 0.6922726935306542 1106 16-17 0.8032270059237732 1106 18-19 0.6706873058112635 1106 20-21 0.8340199863012145 1106 22-23 0.5331413048342242 1106 24-25 0.4360427095064736 1106 26-27 0.7912205437919724 1106 28-29 1.012240819883182 1106 30-31 0.5439472591123646 1106 32-33 0.7178652250194162 1106 34-35 0.37321974379903367 1106 36-37 0.16160701965545599 1106 38-39 0.4389630781195635 1106 40-41 0.23403381651790767 1106 42-43 -0.08596217818725904 1106 44-45 0.729923108198868 1106 46-47 1.0964143607658627 1106 48-49 1.3039422006432417 1106 50-51 1.0344301336298791 1106 52-53 0.7130870869885975 1106 54-55 0.914654925296098 1106 56-57 1.7190143400028326 1106 58-59 2.263788953380999 1106 60-61 1.4124119413883207 1106 62-63 1.428579364889142 1106 64-65 2.1541193703229418 1106 66-67 2.2598991044057612 1106 68-69 2.6539919995633845 1106 70-71 2.2683872913946814 1106 72-73 2.504133279090148 1106 74-75 2.7687811651012844 1106 76-77 2.785567396214443 1106 78-79 2.115125565904574 1106 80-81 2.6077457171842795 1106 82-83 2.4876562675256473 1106 84-85 2.093404866057508 1106 86-87 1.3949090923324725 1106 88-89 1.821043295437768 1106 90-91 2.0465799354862106 1106 92-93 3.0400566732344565 1106 94-95 2.2309430717069674 1106 96-97 2.2529646795669223 1106 98-99 2.1439464978889475 1106 100-101 0.8118995853710551 1107 1 0.21621010924457806 1107 2 0.2762477366450966 1107 3 0.3660682680251526 1107 4 0.5166413985516485 1107 5 0.2530939088104702 1107 6 -0.29203490203227034 1107 7 0.170130600288104 1107 8 0.13463618392195542 1107 9 0.08293054879178641 1107 10-11 0.7035310786805198 1107 12-13 0.46215618880667364 1107 14-15 1.1847330109909748 1107 16-17 0.8996555773523482 1107 18-19 0.4730682581922139 1107 20-21 0.33243268471390763 1107 22-23 -0.12519202849910727 1107 24-25 -0.0032430047792431083 1107 26-27 0.23249038506181563 1107 28-29 0.5130344706768355 1107 30-31 0.5364075765726781 1107 32-33 0.7960398281940186 1107 34-35 -0.2109072403279484 1107 36-37 -0.020139012090574226 1107 38-39 0.18896307811956348 1107 40-41 -0.08342650094240867 1107 42-43 -0.20778757501265233 1107 44-45 -0.026029272753511634 1107 46-47 0.6571286464801602 1107 48-49 -0.06867684697580856 1107 50-51 -0.41914129494155716 1107 52-53 -0.6654843415828324 1107 54-55 -0.3988371381959652 1107 56-57 0.0761571971456867 1107 58-59 0.1542651438571916 1107 60-61 -0.3812388522624772 1107 62-63 -0.32816666685688745 1107 64-65 -0.12088062967705682 1107 66-67 -0.6841485146418584 1107 68-69 -1.379341333769947 1107 70-71 -1.2205015974942057 1107 72-73 -0.8395175145606473 1107 74-75 -1.2832029618828429 1107 76-77 -1.4882421275950826 1107 78-79 -1.601144275365268 1107 80-81 -1.1418574574188938 1107 82-83 -0.8508357959664146 1107 84-85 -0.7022300545774094 1107 86-87 -0.420963923540544 1107 88-89 -0.09721067281619966 1107 90-91 -0.44746768356140976 1107 92-93 -0.07105443787665422 1107 94-95 0.46229227805617157 1107 96-97 0.5303456319478741 1107 98-99 0.0748988788413314 1107 100-101 0.9539630774345476 1108 1 -0.8379142727486553 1108 2 -0.5616393307501646 1108 3 -0.6300285313503373 1108 4 -0.2429214429784068 1108 5 -0.6623367443251027 1108 6 -0.8275540277153297 1108 7 -0.4174312003884566 1108 8 -0.4851920752505663 1108 9 -0.453629430391139 1108 10-11 -0.542760880726199 1108 12-13 -0.20359973104760343 1108 14-15 -0.7775360496387407 1108 16-17 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0.5911295656923592 2316 64-65 0.8108462779534626 2316 66-67 0.6075898674579676 2316 68-69 0.5122249312902944 2316 70-71 0.14429090585251458 2316 72-73 -0.22257354821908848 2316 74-75 -0.06786542124409678 2316 76-77 0.12994490625460386 2316 78-79 0.17988460204915313 2316 80-81 -0.22428239526551863 2316 82-83 -0.7150545758478479 2316 84-85 -0.11693649940433559 2316 86-87 -0.017540706864316746 2316 88-89 0.3168264279678894 2316 90-91 0.7318208993416313 2316 92-93 0.07017715516216683 2316 94-95 -0.08743042226893749 2316 96-97 0.16832612535005254 2316 98-99 -0.5731217752034183 2316 100-101 -1.3192249126209141 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 4.0 5 16.0 6 30.0 7 60.0 8 108.0 9 157.0 10 307.0 11 434.0 12 590.0 13 805.0 14 990.0 15 1273.0 16 1593.0 17 1988.0 18 1677.0 19 1101.0 20 1049.0 21 1035.0 22 1119.0 23 1039.0 24 1073.0 25 1147.0 26 1136.0 27 986.0 28 1149.0 29 1309.0 30 1467.0 31 1678.0 32 1944.0 33 2504.0 34 3399.0 35 8503.0 36 6366.0 37 9183.0 38 8209.0 39 1268.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.10268742860578 22.556063247760477 15.457223591655142 12.884025731978596 2 14.81098265029166 29.440520641204433 36.08349453416707 19.665002174336827 3 22.585046252567505 35.385837868633715 29.967465779097136 12.061650099701644 4 10.064618659949923 24.430651134199913 34.96004437847644 30.544685827373723 5 7.457383169162957 44.98118412569904 31.826564116403546 15.734868588734463 6 22.201232402284894 40.158023358671045 15.82032714133645 21.82041709770761 7 16.192146808797734 44.72630774074574 21.580533441280977 17.50101200917555 8 24.60906460366722 32.20138232957016 21.120256675512376 22.069296391250244 9 24.390170767177917 21.62701089971364 22.340664777582873 31.64215355552557 10-11 21.630759081845305 32.10392959414684 23.021334652693444 23.24397667131441 12-13 27.1195969954572 32.12641868693684 25.828723069311383 14.925261248294577 14-15 23.414144140092056 31.263587160227292 31.49072699740626 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32.7158781681747 38.225747205709276 16.327203760204505 42-43 14.143382986858184 32.49619539549145 37.483038585811634 15.877383031838729 44-45 14.568761947599235 31.618126616439895 37.42868923123056 16.384422204730313 46-47 14.6454839217568 32.68205639568448 37.28023152070416 15.392228161854565 48-49 14.581661955568217 32.08670420549886 36.92126592338779 16.410367915545127 50-51 12.584429701263167 32.65789572322801 37.612354286142654 17.14532028936617 52-53 13.667133957553995 33.60521166777867 34.444835935918675 18.28281843874866 54-55 13.35497316261357 32.15688626345617 34.5887432906534 19.89939728327686 56-57 15.107126257552814 31.54414741292712 33.913069553353225 19.435656776166844 58-59 15.398397265305816 31.35527785482432 34.46179449312953 18.784530386740332 60-61 14.565544952285284 32.900290111471776 33.94379183939669 18.590373096846253 62-63 12.652363603652228 33.178908229508686 34.08671794179823 20.082010225040854 64-65 12.123483814864237 33.57197475148807 33.35007571554943 20.954465718098263 66-67 14.173375912682348 30.99821586530533 32.23511596875515 22.593292253257168 68-69 14.5197079416483 30.325791990884422 32.96301293872472 22.19148712874256 70-71 15.821826414189118 30.2350859832981 32.137663233331835 21.805424369180948 72-73 15.171596923399852 28.64221779090514 34.3223833155914 21.8638019701036 74-75 14.563936490801959 30.012443964677132 32.8813025682544 22.54231697626651 76-77 14.507686415284212 28.938771525342265 33.15484728553512 23.3986947738384 78-79 15.081711361555891 28.926872458243917 33.90908954484746 22.082326635352732 80-81 15.42434760444701 28.060692839954122 33.18015188203279 23.33480767356608 82-83 14.22959864465734 27.702814135144454 33.8018560997916 24.265731120406603 84-85 14.417757387666983 28.22381145144605 33.00274366932038 24.35568749156659 86-87 14.452107739761496 28.239284936996707 32.3789653800836 24.929641943158195 88-89 14.496008924331619 27.71517513247055 31.81403622494743 25.974779718250407 90-91 15.086877739599858 26.712931287264198 32.12107997563965 26.079110997496297 92-93 15.086099982712357 27.28441180670911 32.248220500139055 25.38126771043948 94-95 14.453206992713424 26.43857387028097 32.703379411676515 26.404839725329094 96-97 13.790621837400202 26.807601484313505 33.20514262153754 26.196634056748753 98-99 15.216983890192434 26.851428442918507 31.01793893415894 26.913648732730124 100-101 13.988965351804374 26.569363858528618 30.48246600398807 28.959204785678942 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3764.0 1 2145.5 2 733.5 3 935.5 4 635.0 5 424.0 6 773.5 7 1353.5 8 1652.5 9 1400.5 10 804.5 11 364.5 12 274.5 13 243.5 14 218.5 15 210.5 16 214.5 17 215.0 18 222.0 19 228.5 20 232.5 21 262.5 22 285.0 23 317.0 24 346.5 25 373.5 26 451.0 27 509.5 28 586.0 29 724.0 30 835.0 31 958.5 32 1076.5 33 1139.0 34 1211.0 35 1294.5 36 1352.5 37 1361.5 38 1269.5 39 1140.5 40 1088.0 41 1082.5 42 1084.5 43 1043.0 44 1001.5 45 1006.0 46 1129.5 47 1990.5 48 3483.0 49 3963.5 50 4528.5 51 4679.0 52 3303.0 53 1954.5 54 1005.0 55 635.5 56 510.0 57 454.0 58 405.5 59 352.0 60 322.0 61 280.0 62 195.5 63 119.0 64 82.5 65 60.0 66 57.0 67 60.0 68 52.0 69 30.5 70 19.5 71 18.0 72 14.0 73 11.0 74 7.5 75 5.0 76 2.0 77 1.0 78 0.0 79 0.5 80 1.5 81 1.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2503785663952983 2 0.017991274231997482 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0014992728526664568 26-27 7.496364263332284E-4 28-29 0.0022489092789996852 30-31 0.0 32-33 0.0 34-35 7.496364263332284E-4 36-37 7.496364263332284E-4 38-39 0.0 40-41 7.496364263332284E-4 42-43 0.005247454984332598 44-45 0.0022489092789996852 46-47 0.01424309210033134 48-49 0.01874091065833071 50-51 0.0022489092789996852 52-53 0.003748182131666142 54-55 0.0014992728526664568 56-57 0.0029985457053329136 58-59 7.496364263332284E-4 60-61 7.496364263332284E-4 62-63 0.0 64-65 0.0029985457053329136 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0029985457053329136 74-75 0.0 76-77 0.18066237874630806 78-79 0.09295491686532031 80-81 0.003748182131666142 82-83 0.0 84-85 0.0 86-87 0.11319510037631748 88-89 0.544985681944257 90-91 0.2961063884016252 92-93 0.26612093134829606 94-95 0.0014992728526664568 96-97 0.0022489092789996852 98-99 7.496364263332284E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 66699.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.57033838588285 #Duplication Level Percentage of deduplicated Percentage of total 1 84.22115627366826 35.011019655467095 2 7.963357016626393 6.620788917375073 3 2.762650124427453 3.445329015427517 4 1.4101778050275904 2.3448627415703385 5 0.7285317560500595 1.5142655811931214 6 0.55902189201861 1.3943237529798047 7 0.4075449922458254 1.1859248264591673 8 0.30656039239730226 1.0195055398131905 9 0.19475601399358025 0.728646606395898 >10 1.110830598333754 7.826204290918905 >50 0.15869008547625058 4.482825829472706 >100 0.13344393551411982 11.839757717507009 >500 0.025246149962130777 7.177019145714328 >1k 0.01803296425866484 15.409526379705843 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4083 6.121531057437143 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1936 2.9025922427622604 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1643 2.4633052969309883 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1453 2.178443454924362 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1163 1.7436543276510892 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 975 1.4617910313497953 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 820 1.2294037391864945 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 755 1.1319510037631748 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 600 0.8995637115998741 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 577 0.8650804359885457 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 552 0.8275986146718841 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 508 0.76163060915456 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 452 0.6776713294052384 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 375 0.5622273197499212 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 332 0.4977585870852636 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 331 0.49625931423259717 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 276 0.41379930733594206 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 271 0.40630294307260983 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 269 0.40330439736727686 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 257 0.3853131231352794 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 254 0.38081530457728 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 250 0.3748182131666142 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 250 0.3748182131666142 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 249 0.37331894031394774 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 242 0.36282403034528254 RNA PCR Primer, Index 26 (100% over 22bp) GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 239 0.35832621178728313 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 231 0.34633202896595155 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 225 0.3373363918499528 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 224 0.3358371189972863 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 214 0.32084439047062174 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 213 0.3193451176179553 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 205 0.3073509347966236 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 200 0.29985457053329134 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 194 0.2908589334172926 No Hit TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG 186 0.278864750595961 RNA PCR Primer, Index 26 (100% over 29bp) ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 183 0.2743669320379616 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 182 0.27286765918529515 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 179 0.26836984062729574 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 162 0.24288220213196599 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 148 0.22189238219463558 No Hit ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT 141 0.2113974722259704 RNA PCR Primer, Index 26 (100% over 24bp) GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 133 0.19940328940463875 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 124 0.18590983373064063 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 123 0.1844105608779742 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 122 0.1829112880253077 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 122 0.1829112880253077 No Hit TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 116 0.173915650909309 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 114 0.17091710520397607 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 109 0.1634207409406438 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 98 0.14692873956131278 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 96 0.14393019385597985 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 92 0.137933102445314 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 89 0.13343528388731465 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 88 0.1319360110346482 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 86 0.1289374653293153 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 83 0.1244396467713159 No Hit GTATCAACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 82 0.12294037391864945 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 81 0.12144110106598299 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 81 0.12144110106598299 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 78 0.11694328250798362 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 78 0.11694328250798362 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 78 0.11694328250798362 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 75 0.11244546394998427 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 74 0.11094619109731779 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 73 0.10944691824465134 No Hit GAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 72 0.1079476453919849 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 70 0.10494909968665198 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 69 0.10344982683398551 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0014992728526664568 0.0 12-13 0.0 0.0 0.0 0.0029985457053329136 0.0 14-15 0.0 0.0 0.0 0.005997091410665827 0.0 16-17 0.0 0.0 0.0 0.01949054708466394 0.0 18-19 0.0 0.0 0.0 0.031484729905995594 0.0 20-21 0.0 0.0 0.0 0.05697236840132536 0.0 22-23 0.0 0.0 0.0 0.11544400965531718 0.0 24-25 0.0 0.0 0.0 0.1874091065833071 0.0 26-27 0.0 0.0 0.0 0.2503785663952983 0.0 28-29 0.0 0.0 0.0 0.37256930388761456 0.0 30-31 0.0 0.0 0.0 0.6799202386842381 0.0 32-33 0.0 0.0 0.0 1.0847239089041816 0.0 34-35 0.0 0.0 0.0 1.6919294142340964 0.0 36-37 0.0 0.0 0.0 2.4835454804419856 0.0 38-39 0.0 0.0 0.0 3.3846084648945265 0.0 40-41 0.0 0.0 0.0 4.485074738751706 0.0 42-43 0.0 0.0 0.0 5.420620998815575 0.0 44-45 0.0 0.0 0.0 6.171007061575136 0.0 46-47 0.0 0.0 0.0 7.182266600698661 0.0 48-49 0.0 0.0 0.0 8.219013778317516 0.0 50-51 0.0 0.0 0.0 9.01887584521507 0.0 52-53 0.0 0.0 0.0 9.544370980074664 0.0 54-55 0.0 0.0 0.0 10.068366842081591 0.0 56-57 0.0 0.0 0.0 10.512901242897195 0.0 58-59 0.0 0.0 0.0 10.881722364653143 0.0 60-61 0.0 0.0 0.0 11.286526034873086 0.0 62-63 0.0 0.0 0.0 11.650099701644702 0.0 64-65 0.0 0.0 0.0 11.99043463919999 0.0 66-67 0.0 0.0 0.0 12.344263032429271 0.0 68-69 0.0 0.0 0.0 12.75956161261788 0.0 70-71 0.0 0.0 0.0 13.251323108292478 0.0 72-73 0.0 0.0 0.0 13.66287350634942 0.0 74-75 0.0 0.0 0.0 14.071425358701031 0.0 76-77 0.0 0.0 0.0 14.425253751930313 0.0 78-79 0.0 0.0 0.0 14.834555240708257 0.0 80-81 0.0 0.0 0.0 15.305326916445523 0.0 82-83 0.0 0.0 0.0 15.799337321399122 0.0 84-85 0.0 0.0 0.0 16.25211772290439 0.0 86-87 0.0 0.0 0.0 16.64867539243467 0.0 88-89 0.0 0.0 0.0 17.108952158203273 0.0014992728526664568 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTCTCA 20 0.0020644458 71.25901 1 CGCAGAA 100 2.9744115E-8 37.976295 8 GCCAAAA 25 0.0016156408 37.976295 16-17 ATGGGCA 75 3.0588414E-4 31.646912 5 TGGGCAG 60 0.003936543 31.64691 6 GCAGAAA 130 2.9812327E-7 29.212536 9 GTACAAA 85 6.338841E-4 27.94471 1 CCAAACC 35 0.008193098 27.258844 88-89 CATGGGG 110 9.3857256E-5 25.89293 4 CATGGGC 410 0.0 25.471905 4 CAGAAAA 155 0.0 24.500835 10-11 GTAATGG 145 2.289686E-5 22.933933 1 TAATGGG 125 2.2345196E-4 22.785776 2 CCCAACC 65 2.8467452E-4 22.008465 90-91 TACAAAA 110 0.0028596665 21.57744 2 TGGGCTT 335 1.8189894E-12 21.255388 6 AGTACTT 1235 0.0 21.14065 12-13 GGCTTGA 320 1.6370905E-11 20.768288 8 GGGCTTG 325 2.0008883E-11 20.448772 7 GCTTGAT 325 2.0008883E-11 20.448772 9 >>END_MODULE