##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936306_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 62810 Sequences flagged as poor quality 0 Sequence length 101 %GC 33 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12674733322719 33.0 31.0 34.0 30.0 34.0 2 32.18930106670912 33.0 31.0 34.0 30.0 34.0 3 32.231268906225125 34.0 31.0 34.0 30.0 34.0 4 35.79996815793663 37.0 35.0 37.0 35.0 37.0 5 35.83274956217163 37.0 35.0 37.0 35.0 37.0 6 35.96436873109378 37.0 35.0 37.0 35.0 37.0 7 35.89914026428912 37.0 35.0 37.0 35.0 37.0 8 35.89598790001592 37.0 35.0 37.0 35.0 37.0 9 37.49170514249323 39.0 37.0 39.0 34.0 39.0 10-11 37.53541633497851 39.0 37.0 39.0 35.0 39.0 12-13 37.4779016080242 39.0 37.0 39.0 34.5 39.0 14-15 38.44513612482089 40.0 38.0 41.0 33.5 41.0 16-17 38.35925807992358 40.0 37.5 41.0 33.5 41.0 18-19 38.454601178156345 40.0 37.0 41.0 34.0 41.0 20-21 38.41578570291355 40.0 37.0 41.0 34.0 41.0 22-23 38.23584620283394 40.0 36.5 41.0 34.0 41.0 24-25 38.128323515363796 40.0 36.0 41.0 34.0 41.0 26-27 37.76253781245025 40.0 35.0 41.0 33.0 41.0 28-29 37.43679350421907 39.5 35.0 41.0 33.0 41.0 30-31 36.87551345327177 38.5 35.0 41.0 33.0 41.0 32-33 36.29030409170514 38.0 35.0 41.0 33.0 41.0 34-35 35.91340550867696 37.0 35.0 41.0 31.5 41.0 36-37 35.523658653080716 37.0 35.0 40.5 31.0 41.0 38-39 35.0515761821366 36.5 35.0 40.0 30.0 41.0 40-41 34.56386721859576 36.0 35.0 40.0 26.5 41.0 42-43 34.065093138035344 36.0 35.0 40.0 22.5 41.0 44-45 33.41580162394523 35.0 34.5 40.0 13.5 41.0 46-47 32.6614711033275 35.0 33.0 40.0 2.0 41.0 48-49 31.99789046330202 35.0 33.0 40.0 2.0 41.0 50-51 31.4535742716128 35.0 33.0 40.0 2.0 41.0 52-53 31.0424295494348 35.0 33.0 40.0 2.0 41.0 54-55 30.656654991243432 35.0 31.5 40.0 2.0 41.0 56-57 30.11176564241363 35.0 31.0 40.0 2.0 41.0 58-59 29.57489253303614 35.0 28.5 40.0 2.0 41.0 60-61 29.003980257920713 35.0 26.5 39.5 2.0 41.0 62-63 28.48247890463302 35.0 25.5 39.0 2.0 41.0 64-65 27.94876612004458 35.0 21.0 39.0 2.0 41.0 66-67 27.374852730456933 35.0 13.0 38.0 2.0 40.5 68-69 26.861264129915618 35.0 2.0 37.0 2.0 40.0 70-71 26.352698614870242 35.0 2.0 37.0 2.0 39.5 72-73 25.903494666454385 35.0 2.0 36.0 2.0 39.0 74-75 25.480480815156824 34.0 2.0 35.5 2.0 39.0 76-77 24.79570928196147 33.0 2.0 35.0 2.0 37.0 78-79 24.76362840312052 34.0 2.0 35.0 2.0 37.0 80-81 24.53577455819137 34.0 2.0 35.0 2.0 37.0 82-83 24.252165260308868 34.0 2.0 35.0 2.0 36.0 84-85 23.935686992517116 33.0 2.0 35.0 2.0 36.0 86-87 23.551281643050473 33.0 2.0 35.0 2.0 35.5 88-89 23.05167966884254 33.0 2.0 35.0 2.0 35.0 90-91 22.737080082789365 33.0 2.0 35.0 2.0 35.0 92-93 22.412084063047285 33.0 2.0 35.0 2.0 35.0 94-95 22.23296449609935 32.0 2.0 35.0 2.0 35.0 96-97 22.010109855118614 32.0 2.0 35.0 2.0 35.0 98-99 21.610595446584938 32.0 2.0 35.0 2.0 35.0 100-101 19.7402961311893 28.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5523619925166159 1101 2 0.3174992773184755 1101 3 0.3362663989092596 1101 4 0.4405923452801517 1101 5 0.19915078487409232 1101 6 0.4538254967282995 1101 7 0.4007324658118847 1101 8 0.3014162340575339 1101 9 0.6918316148839736 1101 10-11 0.4662995770278613 1101 12-13 0.6478410403323664 1101 14-15 0.7733908240416127 1101 16-17 0.7895396949692568 1101 18-19 0.8940146197109655 1101 20-21 0.3364739380822215 1101 22-23 0.012776912579560928 1101 24-25 0.14012590357354782 1101 26-27 0.29348447493929086 1101 28-29 0.7453757787812236 1101 30-31 0.7440716097239601 1101 32-33 1.5634052166724288 1101 34-35 1.149859444166097 1101 36-37 1.3285301228284823 1101 38-39 1.3421050904283973 1101 40-41 0.8864339499736857 1101 42-43 0.5660677812617863 1101 44-45 0.3540914345615036 1101 46-47 0.07998835486682054 1101 48-49 0.8622209834042565 1101 50-51 0.6918870563431767 1101 52-53 1.2999539931940767 1101 54-55 0.7529630734464625 1101 56-57 0.4497093488428945 1101 58-59 0.10824071747176944 1101 60-61 -0.14599904946495457 1101 62-63 0.22255291738281002 1101 64-65 1.0488650775365471 1101 66-67 0.33349356811601893 1101 68-69 0.9203356417588502 1101 70-71 1.5380933869954667 1101 72-73 0.9443033729105714 1101 74-75 0.9941376757559084 1101 76-77 1.0129797226217967 1101 78-79 0.7591100687912977 1101 80-81 0.5105421669759878 1101 82-83 0.42642765783487135 1101 84-85 1.3054901601883415 1101 86-87 1.1043636917020372 1101 88-89 1.1639785071842574 1101 90-91 -0.6045532836667711 1101 92-93 0.413227519132235 1101 94-95 0.19783952470208988 1101 96-97 0.5625794238472963 1101 98-99 1.225589380735446 1101 100-101 -0.7138060322386863 1102 1 0.29466968482430644 1102 2 0.5090377388569323 1102 3 0.48549716814002863 1102 4 0.27674619143400037 1102 5 0.3706892464125531 1102 6 0.4745947274975322 1102 7 0.581501696581121 1102 8 0.15910854174983768 1102 9 -0.22047607742371866 1102 10-11 0.3701457308740146 1102 12-13 0.8443795018708329 1102 14-15 0.6556985163493039 1102 16-17 1.1176166180461777 1102 18-19 0.7251684658648117 1102 20-21 0.5464739380822223 1102 22-23 0.5523922971949489 1102 24-25 -0.473335634887988 1102 26-27 -0.12959244813762894 1102 28-29 -0.04923960583415976 1102 30-31 -0.3078514671991215 1102 32-33 -1.6715947833275706 1102 34-35 -2.1493713250646778 1102 36-37 -1.4910852617869068 1102 38-39 -0.5286641403408368 1102 40-41 -0.14971989618015868 1102 42-43 -1.2635476033536008 1102 44-45 -1.2493701039000342 1102 46-47 -1.177703952825489 1102 48-49 -0.6200867089034432 1102 50-51 -0.685420635964519 1102 52-53 -0.8142767760366887 1102 54-55 -0.040113849630458276 1102 56-57 0.546247810381356 1102 58-59 0.9993945636256178 1102 60-61 0.9893855659196618 1102 62-63 1.4794759943058864 1102 64-65 1.3800189236903933 1102 66-67 0.732724337346788 1102 68-69 0.3395664109896188 1102 70-71 0.5592472331493106 1102 72-73 -0.44531201170481793 1102 74-75 -0.17470847809024548 1102 76-77 -0.23278950814743382 1102 78-79 -0.09358223890101058 1102 80-81 0.7720806285144484 1102 82-83 0.5029661193733332 1102 84-85 1.0151055448037276 1102 86-87 0.5947483070866504 1102 88-89 0.1497477379534935 1102 90-91 0.5100621009486126 1102 92-93 0.9932275191322333 1102 94-95 1.4255318323943982 1102 96-97 0.5345025007703761 1102 98-99 0.4209739961200576 1102 100-101 1.0242708908382383 1103 1 -0.3363172527664062 1103 2 0.09938606977130604 1103 3 0.07475696494699946 1103 4 -0.008086900002865605 1103 5 0.10254701128918953 1103 6 -0.2137216730830218 1103 7 0.11960039034018877 1103 8 0.5165105736801721 1103 9 0.49069953941227595 1103 10-11 0.6644127845750276 1103 12-13 0.05501085165312247 1103 14-15 0.1143342202680202 1103 16-17 0.6827472421390723 1103 18-19 0.6549580159373747 1103 20-21 0.678549409780338 1103 22-23 0.7644750257871067 1103 24-25 0.8646542054603401 1103 26-27 1.312352399467592 1103 28-29 0.7363191750076368 1103 30-31 1.6818074587805611 1103 32-33 2.4675561600686606 1103 34-35 1.959482085675532 1103 36-37 2.2072093681114993 1103 38-39 2.1785201847680185 1103 40-41 2.936999987709534 1103 42-43 2.590407403903299 1103 44-45 3.928242377957737 1103 46-47 3.9554600529800226 1103 48-49 3.874107775857084 1103 50-51 3.3205663016261937 1103 52-53 3.5705200309299236 1103 54-55 3.533906469672875 1103 56-57 3.3276338771447804 1103 58-59 3.5471086420000724 1103 60-61 3.501359441101087 1103 62-63 3.511043483420547 1103 64-65 2.9230160209327707 1103 66-67 4.1285879077386625 1103 68-69 3.3297696040229994 1103 70-71 3.9607348964294253 1103 72-73 4.106756203099248 1103 74-75 4.217345222925719 1103 76-77 4.118262741489719 1103 78-79 3.8768459178478984 1103 80-81 3.8341270726363668 1103 82-83 3.897559733306572 1103 84-85 4.198886386603437 1103 86-87 4.623231616230338 1103 88-89 3.789261526052183 1103 90-91 4.394314640861531 1103 92-93 4.648887896490724 1103 94-95 3.9433112228153 1103 96-97 3.6580511219605008 1103 98-99 4.046155418471294 1103 100-101 4.0233637790820715 1104 1 0.19851583867045974 1104 2 0.41288389270308556 1104 3 0.19703562967849564 1104 4 -0.12709996241215293 1104 5 0.23607386179717338 1104 6 0.14767165057445197 1104 7 -0.14926753418811245 1104 8 -0.2639683813270821 1104 9 -0.7589376158852588 1104 10-11 -0.2452388845105986 1104 12-13 -0.07869742120609402 1104 14-15 0.7903139009646907 1104 16-17 1.156078156507725 1104 18-19 0.6867069274032715 1104 20-21 0.6810893226976091 1104 22-23 0.35046922027186866 1104 24-25 0.6708951343427714 1104 26-27 0.30309985955467766 1104 28-29 0.39306808647353364 1104 30-31 0.6440716097239587 1104 32-33 1.3187898320570426 1104 34-35 1.6006286749353222 1104 36-37 1.9992993535977064 1104 38-39 1.7021050904283967 1104 40-41 -0.6112583577186186 1104 42-43 -0.08085529566129424 1104 44-45 -0.11475471928464742 1104 46-47 -0.2257808759024087 1104 48-49 0.5433748295580898 1104 50-51 0.21842551788163433 1104 52-53 0.15687707011715446 1104 54-55 -0.3093446188612283 1104 56-57 0.47894011807366255 1104 58-59 1.3070868713179244 1104 60-61 1.5855394120735085 1104 62-63 0.8448606096905031 1104 64-65 0.7165573852288531 1104 66-67 1.521185875808328 1104 68-69 1.7530279494511554 1104 70-71 1.982324156226234 1104 72-73 1.4969956806028755 1104 74-75 1.8060607526789845 1104 76-77 1.3056720303141027 1104 78-79 1.4929562226374493 1104 80-81 1.3105421669759885 1104 82-83 1.78181227321949 1104 84-85 1.5151055448037276 1104 86-87 0.9505175378558839 1104 88-89 0.3997477379534935 1104 90-91 0.47160056248707605 1104 92-93 0.8105352114399267 1104 94-95 1.1466856785482449 1104 96-97 1.197964039231909 1104 98-99 0.6902047653508312 1104 100-101 0.764655506222855 1105 1 -0.056728916574293464 1105 2 0.36113564095483497 1105 3 -0.04736996472710331 1105 4 0.4351377998256112 1105 5 0.2864235121468184 1105 6 0.4665527694555749 1105 7 -0.17199480691538582 1105 8 0.28323441587571097 1105 9 0.7081952512476093 1105 10-11 0.1299359406642253 1105 12-13 0.46238649487781913 1105 14-15 0.8270271876779702 1105 16-17 0.6149942404238047 1105 18-19 0.6221964378927822 1105 20-21 0.8101103017185878 1105 22-23 0.5291405489431966 1105 24-25 0.24921681266445717 1105 26-27 0.9116662931211081 1105 28-29 0.421739415144863 1105 30-31 0.7258897915421372 1105 32-33 0.9852233984906107 1105 34-35 0.7834958078024599 1105 36-37 1.1094392137375735 1105 38-39 1.233923272246578 1105 40-41 1.2646157681555081 1105 42-43 1.6960677812617888 1105 44-45 2.018636889106965 1105 46-47 2.507261082139543 1105 48-49 2.57222098340425 1105 50-51 2.9137052381613557 1105 52-53 3.243590356830442 1105 54-55 3.719326709810108 1105 56-57 3.3960729852065334 1105 58-59 3.346422535653584 1105 60-61 2.7785464050805047 1105 62-63 2.7252801901100803 1105 64-65 3.0406832593547293 1105 66-67 3.640766295388751 1105 68-69 3.78397200539521 1105 70-71 4.08354793245001 1105 72-73 3.632485191092389 1105 74-75 3.2950467666649956 1105 76-77 2.8275251771672494 1105 78-79 2.2754737051549334 1105 80-81 2.2332694397032604 1105 82-83 1.2891549305621481 1105 84-85 1.0191265238247063 1105 86-87 0.8770909644293106 1105 88-89 0.9530694162751701 1105 90-91 1.4481739890605034 1105 92-93 1.3614093373140506 1105 94-95 1.1132940701566376 1105 96-97 0.7734885147563872 1105 98-99 1.291953017099079 1105 100-101 1.976193967761315 1106 1 -0.4018752956189786 1106 2 -0.6262295362408494 1106 3 -0.571869194311077 1106 4 -1.2431364682791681 1106 5 -0.4510187066513325 1106 6 -0.8197338253055975 1106 7 -0.47553872062879066 1106 8 0.08446708151515736 1106 9 0.14979771657888818 1106 10-11 0.025621610926165772 1106 12-13 0.04173934541711333 1106 14-15 0.31084845116025406 1106 16-17 -0.9135111524883754 1106 18-19 -0.48751080401785174 1106 20-21 0.04647393808222233 1106 22-23 -0.12247732470857642 1106 24-25 -0.17004358795188068 1106 26-27 -0.7404138301454566 1106 28-29 -0.26208184833741655 1106 30-31 -0.6016911021404496 1106 32-33 -0.5847303765479097 1106 34-35 -0.7445473354949215 1106 36-37 -1.2018088602223713 1106 38-39 -1.03942033330042 1106 40-41 -1.6552609652805543 1106 42-43 -1.0293559475517782 1106 44-45 -0.929128904421546 1106 46-47 -1.4166218146247047 1106 48-49 -1.5633722369347325 1106 50-51 -1.046079045351739 1106 52-53 -0.8268256678228703 1106 54-55 -0.7526301468925176 1106 56-57 -0.17588387149608664 1106 58-59 -1.5141321638841596 1106 60-61 -0.37599904946495144 1106 62-63 -0.13558267583752937 1106 64-65 -0.0021518716159931728 1106 66-67 -0.17633694035855285 1106 68-69 -0.39152876502081213 1106 70-71 -0.5263133926655534 1106 72-73 -1.1179000169199398 1106 74-75 -1.1839979174644313 1106 76-77 -1.118545701107017 1106 78-79 -0.9405509481578562 1106 80-81 -0.7614917313290981 1106 82-83 -1.6344197997922478 1106 84-85 -1.7021369584557284 1106 86-87 -1.3193651218572846 1106 88-89 -0.8628011538326881 1106 90-91 -0.6076041311243969 1106 92-93 -0.5703318029016629 1106 94-95 -0.954194373602995 1106 96-97 -0.9343697286950778 1106 98-99 -1.7918682463831992 1106 100-101 -2.1678738288488546 1107 1 0.5309334210880436 1107 2 0.7439278487470418 1107 3 0.46626639890926214 1107 4 0.44844948813729957 1107 5 0.4270079277312391 1107 6 0.06525406815686807 1107 7 0.35073246581188755 1107 8 0.6907019483432464 1107 9 0.34683161488397474 1107 10-11 0.4752281484564307 1107 12-13 0.6060553260466577 1107 14-15 0.5348193954701799 1107 16-17 1.0963254092549732 1107 18-19 0.691514619710965 1107 20-21 0.25183108093936113 1107 22-23 0.6052769125795621 1107 24-25 0.14548304643068377 1107 26-27 0.33812733208215207 1107 28-29 0.38894720735265764 1107 30-31 -0.07021410456175659 1107 32-33 -1.5733804976132788 1107 34-35 -1.5676405558339042 1107 36-37 -1.7493270200286588 1107 38-39 -2.0300377667144645 1107 40-41 -1.7053517643120273 1107 42-43 -2.27178936159536 1107 44-45 -2.47876570829564 1107 46-47 -2.2717973594188976 1107 48-49 -3.097421873738604 1107 50-51 -3.8056129436568256 1107 52-53 -4.6879031496630645 1107 54-55 -4.384894069410677 1107 56-57 -3.9956477940142463 1107 58-59 -4.0253307110996595 1107 60-61 -3.5724276208935244 1107 62-63 -3.070661368331475 1107 64-65 -3.296492065320596 1107 66-67 -3.277577860455409 1107 68-69 -2.5635929296697206 1107 70-71 -2.661906613004536 1107 72-73 -2.721410912803716 1107 74-75 -2.708362324244092 1107 76-77 -3.073448848806777 1107 78-79 -3.5894613597801346 1107 80-81 -2.6894578330240115 1107 82-83 -2.101429485022269 1107 84-85 -1.7266526969545133 1107 86-87 -2.5563505940122475 1107 88-89 -1.9203072071014553 1107 90-91 -1.901338997952486 1107 92-93 -2.221058195153482 1107 94-95 -1.6678747610121931 1107 96-97 -1.5692062904384194 1107 98-99 -3.0872677621217015 1107 100-101 -2.3452346036672544 1108 1 -0.22700308684846604 1108 2 -0.123135643316445 1108 3 -0.23611455347168686 1108 4 -0.23210606741825757 1108 5 -0.17418254845924253 1108 6 -0.13315863025582786 1108 7 -0.026251661172238983 1108 8 -0.6287424961011965 1108 9 -0.6075334644811079 1108 10-11 -0.16862105789277138 1108 12-13 -0.3413653088739821 1108 14-15 -0.3223234616726742 1108 16-17 -0.8114126859831217 1108 18-19 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2316 80-81 1.1824933864881864 2316 82-83 0.013988633444629528 2316 84-85 0.426221867505415 2316 86-87 -0.03588021073698755 2316 88-89 -0.9194361269620792 2316 90-91 -0.3135776739106717 2316 92-93 -0.7689676028189858 2316 94-95 -1.3638677923710816 2316 96-97 -0.5515669176161175 2316 98-99 -0.7553862290206546 2316 100-101 -1.4079523737020985 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 1.0 4 5.0 5 6.0 6 28.0 7 61.0 8 90.0 9 144.0 10 267.0 11 410.0 12 618.0 13 835.0 14 990.0 15 1238.0 16 1593.0 17 1916.0 18 1692.0 19 1223.0 20 1114.0 21 1233.0 22 1130.0 23 1074.0 24 1062.0 25 1056.0 26 977.0 27 833.0 28 1022.0 29 1086.0 30 1201.0 31 1461.0 32 1742.0 33 2075.0 34 2956.0 35 7234.0 36 5586.0 37 7722.0 38 8960.0 39 2166.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.77327288979696 23.111671561741794 15.144936789682035 13.97011875877921 2 14.247495899485644 28.79596159052184 37.26451900568499 19.692023504307528 3 19.78984238178634 36.88106989332909 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15.901816850050157 48-49 13.554828385124324 33.41931990987333 36.42088836872318 16.60496333627917 50-51 13.334500931424841 33.92456254876049 35.64172783288487 17.0992086869298 52-53 13.150122202673334 34.05275015723145 35.629045226930764 17.168082413164452 54-55 12.775933576927056 33.46309077448475 35.559907338857975 18.201068309730218 56-57 13.821487708572155 33.950292956311294 33.986912495223535 18.241306839893006 58-59 13.369792786123119 33.695539687467665 34.37139286254468 18.563274663864544 60-61 13.559146632701799 34.581276866740964 32.91832510746696 18.941251393090273 62-63 13.036140741920077 34.63540837446267 33.47556121636682 18.852889667250437 64-65 13.007586431988791 33.88261329894363 33.38348498236732 19.72631528670026 66-67 13.672185957650058 32.91514090113039 32.97006846043624 20.44260468078331 68-69 14.35519821684445 32.37462187549753 33.2455023085496 20.024677599108422 70-71 14.679191211590512 32.72966088202516 32.32606272886483 20.265085177519502 72-73 14.586169289677517 31.94580437672645 32.81509962664884 20.6529267069472 74-75 14.625855755452955 31.916096163031366 32.366661359656106 21.091386721859575 76-77 14.745757305091233 31.513174684190382 32.90321551614138 20.837852494577007 78-79 14.733067729083665 31.538645418326695 33.45418326693227 20.27410358565737 80-81 14.68010412437609 32.02541016884389 32.47120260470152 20.82328310207851 82-83 14.855118611686038 31.29517592740009 32.1891418563923 21.660563604521574 84-85 14.580480815156822 31.21159051106512 31.923260627288645 22.28466804648941 86-87 15.147481928383 32.009276896224684 30.976385357806063 21.866855817586252 88-89 14.908572709514203 31.2574054504115 31.077272872834406 22.75674896723989 90-91 14.883304987983168 30.666475036130343 31.33159268929504 23.11862728659145 92-93 14.93872989262763 31.07172793677404 30.87374765497146 23.115794515626874 94-95 14.568656015411682 30.522452813666725 31.075713068882894 23.833178102038705 96-97 14.906540568080498 30.378295758502098 30.39262514329385 24.322538530123552 98-99 15.293986530592749 30.800522218153446 29.98933273893869 23.916158512315114 100-101 14.91096092151665 31.0319298832183 29.365780654200403 24.691328541064646 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2802.0 1 1601.5 2 625.0 3 814.5 4 501.5 5 343.0 6 669.0 7 1125.0 8 1395.0 9 1195.0 10 687.0 11 340.0 12 272.0 13 242.5 14 218.5 15 213.0 16 211.0 17 227.5 18 259.0 19 245.0 20 230.5 21 292.0 22 371.5 23 415.0 24 454.0 25 510.0 26 569.0 27 706.5 28 846.5 29 957.0 30 1112.0 31 1331.5 32 1652.0 33 1915.5 34 1982.0 35 2024.0 36 2129.5 37 2073.5 38 1858.5 39 1593.5 40 1466.0 41 1494.0 42 1421.0 43 1398.0 44 1368.0 45 1270.0 46 1166.5 47 1314.0 48 2089.5 49 2597.5 50 3243.0 51 3127.5 52 1650.0 53 755.0 54 489.0 55 391.0 56 326.5 57 300.5 58 301.0 59 268.0 60 184.5 61 126.5 62 124.5 63 100.5 64 63.5 65 41.5 66 20.0 67 20.5 68 21.0 69 15.5 70 12.0 71 6.0 72 6.0 73 10.0 74 6.0 75 1.5 76 1.0 77 1.0 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2579207132622194 2 0.020697341187708964 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.960515841426525E-4 12-13 0.0 14-15 0.0 16-17 7.960515841426525E-4 18-19 0.0 20-21 7.960515841426525E-4 22-23 0.0 24-25 0.0023881547524279576 26-27 0.001592103168285305 28-29 0.001592103168285305 30-31 0.0 32-33 7.960515841426525E-4 34-35 0.001592103168285305 36-37 0.0 38-39 7.960515841426525E-4 40-41 0.0 42-43 0.00318420633657061 44-45 0.0023881547524279576 46-47 0.014328928514567744 48-49 0.015124980098710395 50-51 0.004776309504855915 52-53 0.007164464257283872 54-55 7.960515841426525E-4 56-57 0.00318420633657061 58-59 7.960515841426525E-4 60-61 0.0 62-63 0.0 64-65 7.960515841426525E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 7.960515841426525E-4 74-75 0.0 76-77 0.18149976118452474 78-79 0.0955261900971183 80-81 7.960515841426525E-4 82-83 0.0 84-85 0.0 86-87 0.1170195828689699 88-89 0.5667887279095686 90-91 0.3017035503900653 92-93 0.2825983123706416 94-95 7.960515841426525E-4 96-97 0.00318420633657061 98-99 0.001592103168285305 100-101 7.960515841426525E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 62810.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.55755452953352 #Duplication Level Percentage of deduplicated Percentage of total 1 77.45891485671814 29.866263333864033 2 9.50532661656619 7.330042986785544 3 4.158064249731605 4.8097436713899056 4 2.3205879924023454 3.579047922305365 5 1.399785283673301 2.6986148702435915 6 1.0116442315633 2.3403916573793984 7 0.6606656206127673 1.7831555484795414 8 0.5574366173920224 1.7194714217481293 9 0.45833677430010733 1.5905110651170193 >10 2.1554215872491533 15.101098551186118 >50 0.17342472541085144 4.402165260308868 >100 0.10322900322074491 7.110332749562172 >500 0.02064580064414898 5.438624422862602 >1k 0.016516640515319186 12.230536538767712 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3275 5.214137876134373 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1904 3.0313644324152205 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1391 2.214615507084859 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1112 1.770418723133259 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 982 1.5634453112561695 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 687 1.0937748766120046 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 672 1.0698933290877248 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 546 0.8692883298837766 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 529 0.8422225760229264 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 369 0.5874860690972774 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 323 0.5142493233561535 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 266 0.4234994427638911 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 239 0.38051265722018784 RNA PCR Primer, Index 17 (95% over 21bp) GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 222 0.3534469033593377 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 219 0.3486705938544818 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 210 0.33434166533991405 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 210 0.33434166533991405 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 184 0.29294698296449606 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 184 0.29294698296449606 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 184 0.29294698296449606 No Hit TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG 176 0.28021015761821366 RNA PCR Primer, Index 17 (96% over 28bp) ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT 169 0.26906543544021655 RNA PCR Primer, Index 17 (95% over 23bp) CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 164 0.26110491959879 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 143 0.2276707530647986 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 141 0.22448654672822796 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 137 0.2181181340550868 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 135 0.21493392771851616 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 131 0.20856551504537493 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 122 0.1942365865308072 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 116 0.18468396752109537 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 114 0.18149976118452474 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 106 0.1687629358382423 No Hit TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 101 0.1608024199968158 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 101 0.1608024199968158 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 99 0.15761821366024517 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 91 0.14488138831396274 No Hit GTATCAACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 86 0.13692087247253623 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 80 0.1273682534628244 No Hit TATCAACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 79 0.1257761502945391 No Hit GTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 76 0.12099984078968316 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 76 0.12099984078968316 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 76 0.12099984078968316 No Hit GGTATCAACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 75 0.11940773762139786 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 74 0.11781563445311258 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 73 0.11622353128482726 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 73 0.11622353128482726 No Hit GAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 71 0.11303932494825665 No Hit GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 71 0.11303932494825665 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTAAGTTTTTTTGTTTTGTTTTGG 70 0.11144722177997134 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 69 0.10985511861168604 No Hit GAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 69 0.10985511861168604 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 68 0.10826301544340072 No Hit CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT 65 0.10348670593854482 RNA PCR Primer, Index 17 (96% over 26bp) GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 65 0.10348670593854482 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 64 0.10189460277025952 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 64 0.10189460277025952 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 63 0.10030249960197421 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 63 0.10030249960197421 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0015921031682853048 0.0 10-11 0.0 0.0 0.0 0.0031842063365706097 0.0 12-13 0.0 0.0 0.0 0.0031842063365706097 0.0 14-15 0.0 0.0 0.0 0.0031842063365706097 0.0 16-17 0.0 0.0 0.0 0.0031842063365706097 0.0 18-19 0.0 0.0 0.0 0.011144722177997134 0.0 20-21 0.0 0.0 0.0 0.01990128960356631 0.0 22-23 0.0 0.0 0.0 0.03980257920713262 0.0 24-25 0.0 0.0 0.0 0.07960515841426524 0.0 26-27 0.0 0.0 0.0 0.1289603566311097 0.0 28-29 0.0 0.0 0.0 0.224486546728228 0.0 30-31 0.0 0.0 0.0 0.4768348989014488 0.0 32-33 0.0 0.0 0.0 0.8692883298837765 0.0 34-35 0.0 0.0 0.0 1.406623149180067 0.0 36-37 0.0 0.0 0.0 2.1294379875815954 0.0 38-39 0.0 0.0 0.0 2.89921986944754 0.0 40-41 0.0 0.0 0.0 3.7979621079445947 0.0 42-43 0.0 0.0 0.0 4.483362521891419 0.0 44-45 0.0 0.0 0.0 5.040598630791275 0.0 46-47 0.0 0.0 0.0 5.8064002547365074 0.0 48-49 0.0 0.0 0.0 6.654195191848432 0.0 50-51 0.0 0.0 0.0 7.263970705301704 0.0 52-53 0.0 0.0 0.0 7.678713580640025 0.0 54-55 0.0 0.0 0.0 8.13564718993791 0.0 56-57 0.0 0.0 0.0 8.509791434484955 0.0 58-59 0.0 0.0 0.0 8.858462028339437 0.0 60-61 0.0 0.0 0.0 9.212704983282915 0.0 62-63 0.0 0.0 0.0 9.523165101098552 0.0 64-65 0.0 0.0 0.0 9.848750199012896 0.0 66-67 0.0 0.0 0.0 10.154434007323674 0.0 68-69 0.0 0.0 0.0 10.494348033752587 0.0 70-71 0.0 0.0 0.0 10.89157777423977 0.0 72-73 0.0 0.0 0.0 11.272090431459958 0.0 74-75 0.0 0.0 0.0 11.65101098551186 0.0 76-77 0.0 0.0 0.0 12.054609138672186 0.0 78-79 0.0 0.0 0.0 12.45741124024837 0.0 80-81 0.0 0.0 0.0 12.896035663110968 0.0 82-83 0.0 0.0 0.0 13.35217322082471 0.0 84-85 0.0 0.0 0.0 13.812291036459161 0.0 86-87 0.0 0.0 0.0 14.220665499124344 0.0 88-89 0.0 0.0 0.0 14.638592580799237 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGAGGC 25 5.2745974E-5 75.889175 5 TATATGG 40 7.492359E-6 59.335663 2 TAGAAGG 25 0.005028217 56.91688 4 GGAGGCT 25 0.005028217 56.91688 6 GGCGGCG 25 0.005028217 56.91688 9 ATATGGG 45 1.5124779E-5 52.700813 3 CTAGGAT 25 0.0016219266 37.944588 28-29 TCAGACA 25 0.0016219266 37.944588 48-49 TGTATCA 25 0.0016219266 37.944588 34-35 GTGTACA 65 1.2883803E-4 36.66031 1 TGTACAG 65 1.3190601E-4 36.51425 2 ACAGGCC 65 1.325268E-4 36.48518 5 GTATATG 70 1.9919346E-4 34.041714 1 AGGCCAG 70 2.0488776E-4 33.879097 7 GCGTTGC 35 2.0605614E-4 33.879097 12-13 GGCCAGC 75 3.0718508E-4 31.620491 8 CAGGCCA 75 3.0718508E-4 31.620491 6 TACAGGC 90 2.4009301E-5 31.62049 4 GGCGCTT 45 2.4200346E-5 31.62049 48-49 TGACTCA 45 2.4200346E-5 31.62049 56-57 >>END_MODULE