FastQCFastQC Report
Thu 26 May 2016
SRR936305_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936305_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1961951
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30190.15387744138360235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20800.10601691887310132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20350.046.525411
GTATCAA37600.042.5552831
ATCAACG44450.035.0325133
TCAACGC44850.034.9311334
CAACGCA44750.034.7976535
AACGCAG47450.033.1143156
TATCAAC48300.032.6303752
GTACATG65650.029.9252641
TACATGG65400.028.9472452
ACATGGG66550.027.5950243
ACGCAGA57450.027.0207717
CGCAGAG59200.026.2226858
CATGGGG45150.025.9980664
GTACCTG18400.025.9853271
TACCTGG18000.024.9790612
GTGTAGC11300.021.7845971
GCAGAGT69350.021.7022959
CGCGGAA5800.021.32450582-83
AATTCGC5800.021.3231478-79
ATGGGGA30800.020.8994715
Produced by FastQC (version 0.11.5)