FastQCFastQC Report
Thu 26 May 2016
SRR936305_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936305_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1961951
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33990.1732459169469574No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25080.12783193871814333No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG19670.10025734587662996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22200.051.2172851
GTATCAA41050.038.824271
TACCTGG18550.032.2272452
ATCAACG49350.031.9115453
TAACGCA753.1041488E-431.6231924
TCAACGC50000.031.4966964
CAACGCA51300.030.6060685
TATCAAC52800.030.0129782
AACGCAG52900.029.8597036
GTACATG66500.029.403011
TAAACCG650.005884437429.1906365
GTACCTG21600.029.0731321
TACATGG66450.028.560222
ACATGGG66400.027.6464793
ACGCAGA63450.024.6705747
CGCAGAG64700.023.9739938
TGCACCG9200.023.2017975
GCACCGT9700.021.516816
CATGGGG47300.021.4609814
CGTCTAG1150.00369066720.684441
Produced by FastQC (version 0.11.5)