##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936305_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1961951 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46834044275316 34.0 31.0 34.0 31.0 34.0 2 32.60602634826252 34.0 31.0 34.0 31.0 34.0 3 32.68966961967959 34.0 31.0 34.0 31.0 34.0 4 36.17902536811572 37.0 35.0 37.0 35.0 37.0 5 36.134399381024295 37.0 35.0 37.0 35.0 37.0 6 36.16440726603264 37.0 36.0 37.0 35.0 37.0 7 36.11653553019418 37.0 36.0 37.0 35.0 37.0 8 36.096970821391565 37.0 36.0 37.0 35.0 37.0 9 37.86530856275208 39.0 38.0 39.0 35.0 39.0 10-11 37.79775208453218 39.0 38.0 39.0 35.0 39.0 12-13 37.733663327983216 39.0 38.0 39.0 35.0 39.0 14-15 39.09578399256658 40.0 38.0 41.0 36.0 41.0 16-17 39.01583806119521 40.0 38.0 41.0 35.5 41.0 18-19 39.01218098719081 40.0 38.5 41.0 35.0 41.0 20-21 38.96187799797242 40.0 38.0 41.0 35.0 41.0 22-23 38.84306871068645 40.0 38.0 41.0 35.0 41.0 24-25 39.000513009754066 40.0 38.0 41.0 35.0 41.0 26-27 38.93168789638477 40.0 38.0 41.0 35.0 41.0 28-29 38.83758360937658 40.0 38.0 41.0 35.0 41.0 30-31 38.735606037051895 40.0 38.0 41.0 35.0 41.0 32-33 38.60097627310773 40.0 38.0 41.0 34.0 41.0 34-35 38.47246516350306 40.0 38.0 41.0 34.0 41.0 36-37 38.33591256866252 40.0 38.0 41.0 34.0 41.0 38-39 38.210528448467876 40.0 38.0 41.0 33.5 41.0 40-41 38.07409359357089 40.0 38.0 41.0 33.0 41.0 42-43 38.11622436034335 40.0 38.0 41.0 33.0 41.0 44-45 38.00855806286701 40.0 38.0 41.0 33.0 41.0 46-47 37.82653440376441 40.0 37.5 41.0 33.0 41.0 48-49 37.62000350671347 40.0 37.0 41.0 33.0 41.0 50-51 37.42343488700788 40.0 37.0 41.0 32.0 41.0 52-53 37.199349270190744 40.0 36.0 41.0 32.0 41.0 54-55 36.99163664128207 39.5 36.0 41.0 31.0 41.0 56-57 36.72751434668858 39.0 35.5 41.0 31.0 41.0 58-59 36.45104719740707 39.0 35.0 41.0 31.0 41.0 60-61 36.14810614536245 38.5 35.0 40.0 30.0 41.0 62-63 35.83053552305843 38.0 35.0 40.0 30.0 41.0 64-65 35.433334471656025 37.0 35.0 40.0 29.0 41.0 66-67 35.07042275775491 37.0 34.0 39.5 29.0 41.0 68-69 34.701905399268384 36.0 34.0 39.0 29.0 41.0 70-71 34.307766350943524 36.0 34.0 39.0 28.0 40.0 72-73 33.88419639430343 35.0 34.0 37.5 28.0 40.0 74-75 33.443446344990264 35.0 33.0 37.0 27.0 39.0 76-77 32.736873652807844 35.0 32.5 36.0 26.0 39.0 78-79 32.796169476199964 35.0 33.0 36.0 26.0 38.5 80-81 32.62923819198339 35.0 33.0 36.0 26.0 37.0 82-83 32.352013888216376 35.0 33.0 35.0 26.0 37.0 84-85 32.06917043290072 35.0 33.0 35.0 26.0 36.5 86-87 31.666866552732458 35.0 32.0 35.0 25.0 36.0 88-89 31.18534892053879 34.5 32.0 35.0 23.5 36.0 90-91 30.97041388903189 34.0 32.0 35.0 23.0 35.5 92-93 30.67283484653796 34.0 31.0 35.0 20.0 35.0 94-95 30.500396798900688 34.0 31.0 35.0 20.0 35.0 96-97 30.23582597118888 34.0 31.0 35.0 17.5 35.0 98-99 29.74593606058459 34.0 31.0 35.0 2.0 35.0 100-101 26.26938720691801 30.5 24.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.056321158821035056 1101 2 0.15623178114442737 1101 3 0.24321501508255494 1101 4 0.19737964645153028 1101 5 0.11594342743899944 1101 6 0.16255767927446385 1101 7 0.14094841532388358 1101 8 0.13050802255141036 1101 9 0.20878340071870127 1101 10-11 0.16062026538116925 1101 12-13 0.2799356597393583 1101 14-15 0.3909263370923739 1101 16-17 0.45033699027586493 1101 18-19 0.3742148038662023 1101 20-21 0.34263244096351997 1101 22-23 0.33283587872461595 1101 24-25 0.34129899109841233 1101 26-27 0.32400377891293886 1101 28-29 0.3440783631203317 1101 30-31 0.3172014935531351 1101 32-33 0.37716730129923093 1101 34-35 0.32821981349946583 1101 36-37 0.2755207874287109 1101 38-39 0.32229177291716127 1101 40-41 0.2955490360381745 1101 42-43 0.277311620141397 1101 44-45 0.39016785746974136 1101 46-47 0.3551647141711385 1101 48-49 0.3676116661051907 1101 50-51 0.3228945432706496 1101 52-53 0.3056680677113235 1101 54-55 0.32841629794612714 1101 56-57 0.1311090345507182 1101 58-59 0.001151505567428046 1101 60-61 0.19065944965375792 1101 62-63 0.34979371852650587 1101 64-65 0.2666468013920422 1101 66-67 0.15860464328913082 1101 68-69 0.3688637460628428 1101 70-71 0.4194536598245193 1101 72-73 0.32610446068243704 1101 74-75 0.329584711807577 1101 76-77 0.2593168045470904 1101 78-79 0.25772887175580905 1101 80-81 0.3756820063136104 1101 82-83 0.37247558214816223 1101 84-85 0.5024157346808735 1101 86-87 0.33750923814826805 1101 88-89 0.3549237509508991 1101 90-91 0.6149527609865721 1101 92-93 0.7633406282207602 1101 94-95 0.6792871520022565 1101 96-97 0.5843393383582232 1101 98-99 0.7527652401419864 1101 100-101 0.8784198473208171 1102 1 0.0542064565018876 1102 2 0.06741428374048297 1102 3 0.07646181328262713 1102 4 0.04565651561041051 1102 5 0.0723729878405237 1102 6 0.03378258408581303 1102 7 0.07476526371432612 1102 8 0.07442784775044231 1102 9 0.09570902377166135 1102 10-11 0.13995729809144564 1102 12-13 0.18779467237349223 1102 14-15 0.2024095492765241 1102 16-17 0.24925638279922424 1102 18-19 0.32258307575958867 1102 20-21 0.1751220567475329 1102 22-23 0.23255788460900106 1102 24-25 0.20098227251066447 1102 26-27 0.24409399178936297 1102 28-29 0.17553496575099814 1102 30-31 0.222653747793359 1102 32-33 0.29190856125076436 1102 34-35 0.24720670342677664 1102 36-37 0.19561640875097197 1102 38-39 0.22289535547167816 1102 40-41 0.10922537300597668 1102 42-43 0.1702947025228383 1102 44-45 0.10716011257531477 1102 46-47 0.2026032742265258 1102 48-49 0.3658458085420264 1102 50-51 0.07503628781893212 1102 52-53 6.641736303336643E-4 1102 54-55 0.07785922802573708 1102 56-57 0.10435615725753422 1102 58-59 -0.017772646388266367 1102 60-61 -0.006286400986603269 1102 62-63 0.17311061018451568 1102 64-65 0.14353164476344915 1102 66-67 0.09003095862316002 1102 68-69 0.020105525225183385 1102 70-71 0.11931195854379695 1102 72-73 0.10314128138164591 1102 74-75 0.1092202907276203 1102 76-77 -0.039540390849246876 1102 78-79 0.1005323937357403 1102 80-81 0.19853106740741566 1102 82-83 0.22480337723296628 1102 84-85 0.20875335150330443 1102 86-87 0.2775160527900091 1102 88-89 0.36948599134550264 1102 90-91 0.5700383225995829 1102 92-93 0.6795827318898517 1102 94-95 0.4029101616941908 1102 96-97 0.36160601800515835 1102 98-99 0.629593294832187 1102 100-101 1.1467139153374326 1103 1 -0.0027485098280735087 1103 2 0.046328425154626984 1103 3 0.10205103887184919 1103 4 0.14309969742858897 1103 5 0.1273814053489417 1103 6 0.1673473989006311 1103 7 0.2107816778557421 1103 8 0.17384914404674134 1103 9 0.12118250862014435 1103 10-11 0.2295879799096241 1103 12-13 0.1653567514980736 1103 14-15 0.25185188934386105 1103 16-17 0.29897229189013075 1103 18-19 0.20793135017036235 1103 20-21 0.2776473092727798 1103 22-23 0.24599433242045166 1103 24-25 0.20385474725814134 1103 26-27 0.2667581332035027 1103 28-29 0.3323005381415669 1103 30-31 0.3676926787697923 1103 32-33 0.3203597397019422 1103 34-35 0.28334938019445133 1103 36-37 0.3197115265960875 1103 38-39 0.2903195978959232 1103 40-41 0.30128661037971227 1103 42-43 0.4334049718831139 1103 44-45 0.4560132270534254 1103 46-47 0.48648374560699637 1103 48-49 0.399626322010036 1103 50-51 0.5164188635765115 1103 52-53 0.4814406887818379 1103 54-55 0.3924004738169842 1103 56-57 0.4241846505235358 1103 58-59 0.4499567307161101 1103 60-61 0.3580075804948777 1103 62-63 0.37057483577374484 1103 64-65 0.33526670368600264 1103 66-67 0.32878306300026594 1103 68-69 0.2271236228682767 1103 70-71 0.25699083059766537 1103 72-73 0.21909245983281522 1103 74-75 0.4259658715356949 1103 76-77 0.3031942471978937 1103 78-79 0.16545242740577493 1103 80-81 0.3658237862626379 1103 82-83 0.3258660876706827 1103 84-85 0.25846926059421094 1103 86-87 0.16904067400213663 1103 88-89 0.3875888953859068 1103 90-91 0.617034324283086 1103 92-93 0.6748741881188067 1103 94-95 0.6166517441857771 1103 96-97 0.5200498311364719 1103 98-99 0.5314451466840424 1103 100-101 0.969520102206122 1104 1 0.08846072861116028 1104 2 0.10279908446174346 1104 3 0.012653496011800769 1104 4 0.03512035164846594 1104 5 0.020191854552784605 1104 6 0.04286502620896471 1104 7 0.06122903757492537 1104 8 0.10962838299469979 1104 9 -0.09369418924830342 1104 10-11 0.1266974701675494 1104 12-13 0.1423457058145985 1104 14-15 0.20698412595168492 1104 16-17 0.1219393010980454 1104 18-19 0.17471942184966593 1104 20-21 0.1822608057371511 1104 22-23 0.10067205536902435 1104 24-25 0.08473957836157098 1104 26-27 0.18243241590178627 1104 28-29 0.18699861007523566 1104 30-31 0.13395485636555549 1104 32-33 0.08979556409020262 1104 34-35 0.08634099895621716 1104 36-37 0.18398792940424613 1104 38-39 0.1634265063763607 1104 40-41 0.22010671899242595 1104 42-43 0.2625035436387222 1104 44-45 0.319665488334401 1104 46-47 0.3918196310422104 1104 48-49 0.40981385062579534 1104 50-51 0.49301451016550857 1104 52-53 0.4833924426099969 1104 54-55 0.36397882376865454 1104 56-57 0.4217961883980834 1104 58-59 0.33134631203574116 1104 60-61 0.22667713841317294 1104 62-63 0.2863623097016017 1104 64-65 0.2524391804118551 1104 66-67 0.2061301912497413 1104 68-69 0.35453926996600416 1104 70-71 0.4187414156309899 1104 72-73 0.1671429316749098 1104 74-75 0.3406112586751391 1104 76-77 0.33579300336618445 1104 78-79 0.33681870277840886 1104 80-81 0.3693948741702542 1104 82-83 0.3358633214805167 1104 84-85 0.4360284634227227 1104 86-87 0.39544093853894324 1104 88-89 0.5691888025436604 1104 90-91 0.7342899456902181 1104 92-93 0.794904584415292 1104 94-95 0.7307016055583127 1104 96-97 0.5841036669201607 1104 98-99 0.5721348500975836 1104 100-101 0.5355411125332274 1105 1 0.00720962414321491 1105 2 0.05791135976387807 1105 3 0.09900079963740183 1105 4 0.0991104629788282 1105 5 0.08138858238243074 1105 6 0.08598774977782142 1105 7 0.14064611166754304 1105 8 0.07384914404673992 1105 9 0.13161911149095573 1105 10-11 0.16257242967039076 1105 12-13 0.178763848786744 1105 14-15 0.29125579683987723 1105 16-17 0.25693182139571746 1105 18-19 0.11377764203638918 1105 20-21 0.11746489300484342 1105 22-23 0.04338268967723735 1105 24-25 0.2180692608147652 1105 26-27 0.17373580465486071 1105 28-29 0.0723124998640543 1105 30-31 0.12194407430407495 1105 32-33 0.1270084637848825 1105 34-35 0.11416576775425824 1105 36-37 0.290036486723686 1105 38-39 0.2453734256471236 1105 40-41 0.3392600952281981 1105 42-43 0.22387048224993578 1105 44-45 0.10411430360845486 1105 46-47 0.18033841067876466 1105 48-49 0.21417975103715037 1105 50-51 0.2822731299241994 1105 52-53 0.2531312788934841 1105 54-55 0.2032856412810986 1105 56-57 0.24316890092225663 1105 58-59 0.25147686628229593 1105 60-61 0.3001676682141792 1105 62-63 0.28638424566209864 1105 64-65 0.2736638328726144 1105 66-67 0.15971906778495537 1105 68-69 0.2547851061218651 1105 70-71 0.19082755308570398 1105 72-73 0.17179381549470207 1105 74-75 0.11383469131241242 1105 76-77 0.14173092981352653 1105 78-79 0.08012547365776612 1105 80-81 0.0731503412865635 1105 82-83 0.20128973200879585 1105 84-85 0.29181256202961947 1105 86-87 0.3596158334917696 1105 88-89 0.35308431005895 1105 90-91 0.3801473625605958 1105 92-93 0.5031237893947242 1105 94-95 0.21489237416982476 1105 96-97 0.07957933671860573 1105 98-99 0.20458111159632253 1105 100-101 0.2724905966239852 1106 1 0.013257029790082697 1106 2 0.04183923235613207 1106 3 0.00583603244193398 1106 4 0.009322864903118955 1106 5 0.055670553632644726 1106 6 0.02953455885710099 1106 7 0.047166953352821395 1106 8 -0.005380997411364774 1106 9 0.015113040819173307 1106 10-11 0.030760820942369094 1106 12-13 0.07173813195112899 1106 14-15 0.10446064144594658 1106 16-17 0.07806146985036122 1106 18-19 0.17186602471780077 1106 20-21 0.15493858437417884 1106 22-23 0.01573416821034357 1106 24-25 0.010508780792612527 1106 26-27 -0.05961966133601493 1106 28-29 -0.005779390634771175 1106 30-31 -0.10981467853558513 1106 32-33 0.09393773950409923 1106 34-35 0.17928678745531101 1106 36-37 0.08739440818675348 1106 38-39 0.1297460990335253 1106 40-41 -0.018613045867191147 1106 42-43 -0.06411178298878895 1106 44-45 -0.06245422768986231 1106 46-47 0.04746282365625376 1106 48-49 0.03336667713912789 1106 50-51 0.1910704735280433 1106 52-53 -0.04702126343894264 1106 54-55 0.0036308749446902766 1106 56-57 0.0952055230133908 1106 58-59 0.06911540120774617 1106 60-61 6.21835119467562E-4 1106 62-63 -0.03785113365943715 1106 64-65 -0.0015324975208415026 1106 66-67 0.022097189026574426 1106 68-69 -0.03563264629781315 1106 70-71 0.048899649471941586 1106 72-73 -0.08722956756850664 1106 74-75 0.08575634289785228 1106 76-77 -0.1464428336211796 1106 78-79 -0.280834606425465 1106 80-81 -0.12393441187763443 1106 82-83 -0.03331106882352941 1106 84-85 -0.10277752444881827 1106 86-87 -0.012980297409480812 1106 88-89 -0.022367888173299377 1106 90-91 0.22508840975148203 1106 92-93 0.3045049626761305 1106 94-95 0.38895767207181464 1106 96-97 0.4936061918051813 1106 98-99 0.36290597366989275 1106 100-101 0.42786662510650686 1107 1 -2.899620865122188E-4 1107 2 0.08713388541763578 1107 3 0.0345052984487495 1107 4 -0.010902589592561185 1107 5 0.07082051341068762 1107 6 0.09121818220537392 1107 7 0.044840425711662135 1107 8 0.04701238018167686 1107 9 0.07601026733941296 1107 10-11 0.04980310283701783 1107 12-13 0.12146559941115953 1107 14-15 0.11585810718262479 1107 16-17 0.15735565381122996 1107 18-19 0.008269043137779875 1107 20-21 0.15302609715156734 1107 22-23 0.039476322128855657 1107 24-25 0.14558752313006806 1107 26-27 0.09981809603941372 1107 28-29 0.0441930481472923 1107 30-31 0.038649296837256486 1107 32-33 0.04883315308101288 1107 34-35 0.06452791078336162 1107 36-37 0.1317505488945443 1107 38-39 0.12766236519151875 1107 40-41 0.05229254234083669 1107 42-43 -0.022582378031124506 1107 44-45 -0.09626664716041233 1107 46-47 -0.015553132609134934 1107 48-49 -0.11625060766405682 1107 50-51 0.038390347281492154 1107 52-53 0.020693476088872842 1107 54-55 0.036941997544822414 1107 56-57 0.052743827195918414 1107 58-59 -0.017982091467992234 1107 60-61 0.1428489184022581 1107 62-63 0.15607905246898923 1107 64-65 0.1429693194664594 1107 66-67 -0.028013677994586317 1107 68-69 0.0844260099466041 1107 70-71 0.21319794895101296 1107 72-73 0.17493329745431652 1107 74-75 0.1610698595288369 1107 76-77 0.12287334954208973 1107 78-79 0.019431486868320746 1107 80-81 0.281866882317523 1107 82-83 0.26537616485764914 1107 84-85 0.19194910622028516 1107 86-87 0.1827342131673717 1107 88-89 0.41996275187532817 1107 90-91 0.271876376884574 1107 92-93 0.4645754459804401 1107 94-95 0.5621152233167059 1107 96-97 0.44411000838060843 1107 98-99 0.4202315960836991 1107 100-101 0.41959157161721095 1108 1 -0.1967515655958394 1108 2 -0.04740247629686678 1108 3 -0.0486938045200489 1108 4 0.014537818131415747 1108 5 -0.06182791474273586 1108 6 -0.078661004460713 1108 7 0.028357117122354225 1108 8 0.03918527850051845 1108 9 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-0.6770819175057952 2316 76-77 -0.6008684117658447 2316 78-79 -0.6942109782333503 2316 80-81 -0.7814495314468317 2316 82-83 -0.9300643952157372 2316 84-85 -0.7051030633648026 2316 86-87 -0.8394216470501128 2316 88-89 -0.7790389387104533 2316 90-91 -0.782719054831805 2316 92-93 -0.8653708885471332 2316 94-95 -0.838667409582122 2316 96-97 -0.6205644955839738 2316 98-99 -0.871622399279186 2316 100-101 -0.8985436286273973 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 22.0 5 54.0 6 80.0 7 147.0 8 320.0 9 537.0 10 962.0 11 1454.0 12 2149.0 13 3171.0 14 4073.0 15 5444.0 16 6834.0 17 9117.0 18 8896.0 19 6306.0 20 6232.0 21 6516.0 22 6996.0 23 8138.0 24 9835.0 25 11910.0 26 14727.0 27 18596.0 28 23291.0 29 29026.0 30 36013.0 31 44420.0 32 56125.0 33 73333.0 34 101277.0 35 149839.0 36 256941.0 37 479604.0 38 500706.0 39 78811.0 40 38.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.17524224211947 17.542417923872602 12.64391430557259 24.63842552843534 2 16.41506779201341 18.697402308741694 39.212362636802425 25.67516726244247 3 20.418144999543824 22.33689832212935 27.79432309981238 29.450633578514445 4 13.385400552817067 14.711019796111117 34.63480994173656 37.268769709335245 5 14.854754272660225 36.327563736301265 33.41174167958323 15.405940311455282 6 36.079902097452994 33.488807824456366 16.098771070225503 14.33251900786513 7 31.88647422896902 30.32104267639712 18.794862868644525 18.997620225989333 8 27.98204440375932 35.22075729720059 18.087403813856717 18.70979448518337 9 27.082939380239363 15.811047268764614 18.732221141098833 38.37379220989719 10-11 26.888732071676852 25.371971531304784 25.947472010548715 21.791824386469646 12-13 26.947718824495436 21.973761805225315 26.979982660099726 24.098536710179523 14-15 23.31141297616505 23.79330574514858 24.54656105070922 28.348720227977154 16-17 24.253380449890745 27.253187274044148 24.96548064852037 23.527951627544734 18-19 23.836298663931974 25.938950565024303 26.708592620304994 23.51615815073873 20-21 24.349856010601698 25.331863707026177 26.835750146537883 23.482530135834246 22-23 24.063686638293536 25.123499579499985 26.847651571140958 23.965162211065522 24-25 24.054420562050517 25.43942610895377 26.857078488235036 23.64907484076068 26-27 23.991590813404542 25.888219697008314 26.64755935821463 23.472630131372515 28-29 23.58009324601013 25.79133465551629 26.904792123174033 23.723779975299543 30-31 23.755984531581863 25.355096223979107 27.010020219854603 23.878899024584427 32-33 23.633763217905706 25.488526047416766 27.115654954682196 23.762055779995332 34-35 24.173163068107534 25.65609853003653 26.568597743179573 23.602140658676362 36-37 24.035933637452533 25.537526440531106 26.592242411886136 23.834297510130227 38-39 23.865516450470196 25.733938173755703 26.744412446800375 23.656132928973726 40-41 24.11594360979573 25.454187737472644 26.737791972370157 23.69207668036147 42-43 23.992400137113556 25.41723425647116 26.65938622569452 23.930979380720764 44-45 23.95271448992912 25.75617287490325 26.554314647330006 23.736797987837623 46-47 23.818015123350353 25.989126093425064 26.195047736149103 23.997811047075487 48-49 24.26491222838284 25.868865220080917 26.086130909459797 23.780091642076442 50-51 23.934329631769547 25.873719338871876 26.054188732603283 24.137762296755298 52-53 24.275819602773772 25.815270026975174 26.054616057465473 23.85429431278558 54-55 24.05094647317466 25.39884599005628 26.339750701027526 24.210456835741535 56-57 23.93800342273301 26.233044027866093 26.129903294412383 23.699049254988513 58-59 24.169443068950518 25.832735917313716 26.088862372933878 23.908958640801885 60-61 24.208176047414632 25.684888216986778 26.483822529493978 23.623113206104613 62-63 24.201827670517766 25.87077862800855 26.293470122342505 23.63392357913118 64-65 23.941663729516083 25.65746937144468 26.327165189681313 24.073701709357927 66-67 23.80309192227533 26.172366180399 26.07096201689033 23.953579880435342 68-69 23.693660035342372 25.851690485644134 26.465747615511294 23.9889018635022 70-71 23.671842307666825 25.88260539148101 26.36294456476143 24.08260773609073 72-73 23.988746749361088 25.848428224975308 26.224870305751196 23.937954719912412 74-75 23.733390895083517 25.88787895314409 26.151927341712405 24.226802810059986 76-77 23.789079984465417 26.026223418665452 26.033628134652464 24.15106846221667 78-79 23.81924962096851 25.962872644143577 26.131348091857387 24.086529643030527 80-81 23.565549695388846 26.382065596205774 26.151985738514803 23.90039896989058 82-83 23.859540834608 26.2399519661806 25.98742272360523 23.91308447560617 84-85 23.936983833866563 25.947085055055176 25.81038369655357 24.305547414524696 86-87 23.901337373640466 25.846951423948006 26.265636585668307 23.98607461674322 88-89 23.715819791811928 25.976240474452254 26.123173842784368 24.18476589095145 90-91 23.802489005821553 26.178990312239016 26.12310872394419 23.895411957995247 92-93 23.80527241312327 25.92829538599953 26.197948083548884 24.068484117328317 94-95 23.81839761068535 25.86513645053026 26.126413583964332 24.190052354820054 96-97 23.82259791369919 25.932651288156062 25.9455979644155 24.299152833729252 98-99 23.610317873547356 25.71830369237113 26.05626749423326 24.615110939848254 100-101 23.52865470994871 25.891395622949254 26.1491000605269 24.430849606575133 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 58.0 1 48.5 2 73.0 3 127.0 4 101.0 5 83.5 6 199.0 7 411.0 8 654.5 9 779.5 10 730.5 11 710.0 12 773.0 13 842.0 14 960.5 15 1093.0 16 1182.5 17 1237.5 18 1284.5 19 1375.5 20 1552.0 21 1839.0 22 2226.0 23 2757.5 24 3467.0 25 4296.0 26 5359.5 27 6661.0 28 8168.5 29 9862.0 30 12080.5 31 14938.0 32 18040.5 33 21131.5 34 25071.0 35 28658.0 36 32345.5 37 36873.5 38 39904.0 39 42189.5 40 44704.0 41 48689.5 42 53674.0 43 59007.0 44 63118.0 45 65797.0 46 68341.5 47 72256.0 48 79674.0 49 84368.5 50 84949.5 51 84595.0 52 83397.0 53 83737.0 54 84393.5 55 81274.5 56 75183.0 57 69702.5 58 64084.5 59 57374.0 60 48958.0 61 39057.5 62 31590.0 63 25248.5 64 19813.0 65 15771.0 66 11639.0 67 9227.5 68 6497.5 69 3768.0 70 2843.0 71 2173.5 72 1717.5 73 1488.5 74 1273.0 75 1058.5 76 670.0 77 345.5 78 183.5 79 83.5 80 48.5 81 29.0 82 16.5 83 5.5 84 1.0 85 2.0 86 2.5 87 1.5 88 0.5 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2666223570313428 2 0.018501991130257585 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.313028714784416E-4 12-13 1.0193934507028973E-4 14-15 0.0 16-17 4.077573802811589E-4 18-19 0.0 20-21 5.096967253514486E-5 22-23 5.096967253514486E-5 24-25 0.002319120100349091 26-27 0.0024975139542220983 28-29 0.002242665591546374 30-31 9.174541056326075E-4 32-33 0.001478120503519201 34-35 0.0016055446848570632 36-37 5.096967253514486E-5 38-39 5.096967253514486E-5 40-41 7.390602517596005E-4 42-43 0.003746270931333147 44-45 0.004154028311614306 46-47 0.014398932491178424 48-49 0.015163477579205597 50-51 0.006243784885555246 52-53 0.007492541862666294 54-55 4.842118890838762E-4 56-57 0.0034914225686574233 58-59 0.0015036053397867735 60-61 0.0012742418133786216 62-63 0.0 64-65 7.900299242947454E-4 66-67 0.0 68-69 0.0 70-71 2.0387869014057945E-4 72-73 0.001478120503519201 74-75 0.0 76-77 0.1906010904451742 78-79 0.10303519302979533 80-81 0.0011977873045759043 82-83 0.0 84-85 5.096967253514486E-4 86-87 0.12049230587308246 88-89 0.5814110546083975 90-91 0.307321640550656 92-93 0.2921071932989152 94-95 0.0025229987904896705 96-97 0.0029307561707708294 98-99 0.0027778471531653953 100-101 6.880905792244557E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1961951.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.819044524242344 #Duplication Level Percentage of deduplicated Percentage of total 1 75.92059661784035 38.58212179829071 2 13.456410729479359 13.676838719958075 3 4.328075140353412 6.598459297856593 4 1.9645339925425311 3.993429617456258 5 1.043523002412457 2.6515420960834852 6 0.6445338886831168 1.9652757831822223 7 0.4416409440440524 1.5710639559372186 8 0.31844921471670207 1.2946627857118471 9 0.24875820122484452 1.1377488693434228 >10 1.4106963902393914 13.569743444790886 >50 0.13516054424820695 4.730983186878093 >100 0.08129922450148258 7.425007748291828 >500 0.004616143600796058 1.5358374835233306 >1k 0.0017059661133376736 1.2672852126960672 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3399 0.1732459169469574 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2508 0.12783193871814333 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 1967 0.10025734587662996 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 5.096967253514486E-5 0.0 22-23 0.0 0.0 0.0 1.7839385387300702E-4 0.0 24-25 0.0 0.0 0.0 2.548483626757243E-4 0.0 26-27 0.0 0.0 0.0 2.548483626757243E-4 0.0 28-29 0.0 0.0 0.0 6.116360704217384E-4 0.0 30-31 0.0 0.0 0.0 0.0015545750123219183 0.0 32-33 0.0 0.0 0.0 0.0038227254401358647 0.0 34-35 0.0 0.0 0.0 0.006473148411963398 0.0 36-37 0.0 0.0 0.0 0.010958479595056145 0.0 38-39 0.0 0.0 0.0 0.019113627200679325 0.0 40-41 0.0 0.0 0.0 0.029740803924257028 0.0 42-43 0.0 0.0 0.0 0.041310919589734915 0.0 44-45 0.0 0.0 0.0 0.05349267132563454 0.0 46-47 0.0 0.0 0.0 0.06870711857737527 0.0 48-49 0.0 0.0 0.0 0.0862406859294651 0.0 50-51 0.0 0.0 0.0 0.10560916149282015 0.0 52-53 0.0 0.0 0.0 0.12650672723222955 0.0 54-55 0.0 0.0 0.0 0.14959598889065018 0.0 56-57 0.0 0.0 0.0 0.17625312762653095 0.0 58-59 0.0 0.0 0.0 0.2052293864627608 0.0 60-61 0.0 0.0 0.0 0.24154527814405152 0.0 62-63 0.0 0.0 0.0 0.2790844419661857 0.0 64-65 0.0 0.0 0.0 0.3153493639749413 0.0 66-67 0.0 0.0 0.0 0.35984588809812273 0.0 68-69 0.0 0.0 0.0 0.41252304466319495 0.0 70-71 0.0 0.0 0.0 0.4743237726120581 0.0 72-73 0.0 0.0 0.0 0.5368125911401458 0.0 74-75 0.0 0.0 0.0 0.6033789834710449 0.0 76-77 0.0 0.0 0.0 0.6767753119216535 0.0 78-79 0.0 0.0 0.0 0.7640353913018215 0.0 80-81 0.0 0.0 0.0 0.8622794351133132 0.0 82-83 0.0 0.0 0.0 0.9648813859265598 0.0 84-85 0.0 0.0 0.0 1.0676107609211443 0.0 86-87 0.0 0.0 0.0 1.1730160437238237 0.0 88-89 0.0 0.0 0.0 1.300134407026475 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2220 0.0 51.217285 1 GTATCAA 4105 0.0 38.82427 1 TACCTGG 1855 0.0 32.227245 2 ATCAACG 4935 0.0 31.911545 3 TAACGCA 75 3.1041488E-4 31.623192 4 TCAACGC 5000 0.0 31.496696 4 CAACGCA 5130 0.0 30.606068 5 TATCAAC 5280 0.0 30.012978 2 AACGCAG 5290 0.0 29.859703 6 GTACATG 6650 0.0 29.40301 1 TAAACCG 65 0.0058844374 29.190636 5 GTACCTG 2160 0.0 29.073132 1 TACATGG 6645 0.0 28.56022 2 ACATGGG 6640 0.0 27.646479 3 ACGCAGA 6345 0.0 24.670574 7 CGCAGAG 6470 0.0 23.973993 8 TGCACCG 920 0.0 23.201797 5 GCACCGT 970 0.0 21.51681 6 CATGGGG 4730 0.0 21.460981 4 CGTCTAG 115 0.003690667 20.68444 1 >>END_MODULE