FastQCFastQC Report
Thu 26 May 2016
SRR936304_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936304_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1542108
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61660.39984229379524655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43240.28039540680678654No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29250.18967543129274994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68850.035.81991
GTACCTG15800.035.0525321
TATCACG958.9594687E-734.8700332
TACCTGG15550.034.693842
GGTATCA42400.033.7156641
GTACATG84300.031.388811
TACATGG83750.031.2477112
ACATGGG83800.030.157083
ATCAACG82700.029.4136983
TCAACGC83350.029.1843194
CAACGCA84400.028.8212415
AACGCAG86700.028.0566646
TATCAAC87600.027.9295752
GTGTAGC10550.024.22881
CATGGGA48750.024.0751554
ACGCAGA101650.023.7906077
ATAACGC1201.7545068E-423.6625773
ACCTGGG22000.023.6625753
CGCAGAG103150.023.4905268
CATGGGG35700.023.3311674
Produced by FastQC (version 0.11.5)