##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936304_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1542108 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.68678588010697 33.0 31.0 34.0 30.0 34.0 2 31.772497127308853 33.0 31.0 34.0 30.0 34.0 3 31.839736905586378 34.0 31.0 34.0 30.0 34.0 4 35.33036920890106 37.0 35.0 37.0 33.0 37.0 5 35.33040357744075 37.0 35.0 37.0 33.0 37.0 6 35.42654600066921 37.0 35.0 37.0 33.0 37.0 7 35.3754899138063 37.0 35.0 37.0 33.0 37.0 8 35.380265843896794 37.0 35.0 37.0 33.0 37.0 9 37.03871583572616 39.0 37.0 39.0 33.0 39.0 10-11 36.95490944862487 39.0 37.0 39.0 33.0 39.0 12-13 36.85521571770589 39.0 37.0 39.0 33.0 39.0 14-15 38.06540981565493 40.0 38.0 41.0 33.0 41.0 16-17 38.019049573700414 40.0 38.0 41.0 33.0 41.0 18-19 37.99095912867322 40.0 38.0 41.0 33.0 41.0 20-21 37.921894251245696 40.0 38.0 41.0 33.0 41.0 22-23 37.76020194435149 40.0 38.0 41.0 32.5 41.0 24-25 37.91817337047729 40.0 38.0 41.0 33.0 41.0 26-27 37.86977014580042 40.0 38.0 41.0 33.0 41.0 28-29 37.73287571298508 40.0 38.0 41.0 32.5 41.0 30-31 37.56452725749429 40.0 37.5 41.0 32.0 41.0 32-33 37.36195389687363 40.0 37.0 41.0 32.0 41.0 34-35 37.086450819268165 40.0 37.0 41.0 31.0 41.0 36-37 36.87768496110519 40.0 37.0 41.0 31.0 41.0 38-39 36.7189084681488 40.0 36.0 41.0 30.0 41.0 40-41 36.52754962687438 40.0 36.0 41.0 30.0 41.0 42-43 36.32987508008518 40.0 36.0 41.0 30.0 41.0 44-45 36.119073696524495 40.0 35.0 41.0 29.5 41.0 46-47 35.800048375340765 39.5 35.0 41.0 27.5 41.0 48-49 35.5907585590633 39.0 35.0 41.0 27.0 41.0 50-51 35.26655493648953 39.0 35.0 40.5 26.5 41.0 52-53 35.28452157695829 39.0 35.0 40.5 26.5 41.0 54-55 35.371699323264 39.0 35.0 41.0 26.5 41.0 56-57 35.23314450090396 39.0 35.0 41.0 26.0 41.0 58-59 35.01957807105599 39.0 35.0 41.0 26.0 41.0 60-61 34.744775981967535 38.5 35.0 41.0 25.5 41.0 62-63 34.46187361715262 38.0 34.0 40.0 25.0 41.0 64-65 34.143270121158835 37.5 34.0 40.0 23.5 41.0 66-67 33.79986323915057 37.0 34.0 40.0 23.0 41.0 68-69 33.42575909080298 36.5 34.0 39.0 22.0 41.0 70-71 33.029618548117256 36.0 33.0 39.0 21.5 41.0 72-73 32.61951335444729 35.5 33.0 39.0 20.0 40.0 74-75 32.194806394882846 35.0 33.0 37.5 18.5 40.0 76-77 31.78209729798432 35.0 32.0 37.0 17.5 39.0 78-79 31.381872086779914 35.0 32.0 36.5 13.5 39.0 80-81 30.97195592007823 35.0 31.5 36.0 9.0 38.5 82-83 30.611652361572602 35.0 31.0 36.0 7.0 37.0 84-85 30.27522942621399 35.0 31.0 35.0 2.0 37.0 86-87 29.942600647944243 34.0 31.0 35.0 2.0 36.0 88-89 29.61494558098395 34.0 30.5 35.0 2.0 36.0 90-91 29.259096314914387 34.0 30.0 35.0 2.0 36.0 92-93 28.954391650909017 34.0 30.0 35.0 2.0 35.0 94-95 28.581682994965334 34.0 29.0 35.0 2.0 35.0 96-97 28.158911049031584 34.0 29.0 35.0 2.0 35.0 98-99 27.668761202198546 34.0 29.0 35.0 2.0 35.0 100-101 24.42295870328148 30.0 14.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37708447462994243 1101 2 0.27878310310696364 1101 3 0.30989299001310044 1101 4 0.15807076622343175 1101 5 0.4926066941797629 1101 6 0.3413992066237199 1101 7 0.3955544732230365 1101 8 0.32641895153992095 1101 9 0.3846422987775142 1101 10-11 0.5754805744906193 1101 12-13 0.5123304391374575 1101 14-15 0.6308136512400537 1101 16-17 0.6936009190940879 1101 18-19 0.7037714585509818 1101 20-21 0.6088384784387202 1101 22-23 0.6789602096599836 1101 24-25 0.5399934268800308 1101 26-27 0.4412246223572538 1101 28-29 0.38050279643116625 1101 30-31 0.5360604645458409 1101 32-33 0.5560032992206914 1101 34-35 0.37902284733318936 1101 36-37 0.5324293274417684 1101 38-39 0.4915735137872659 1101 40-41 0.44207217943451127 1101 42-43 0.4029628545934756 1101 44-45 0.5394280253587524 1101 46-47 0.5649413858028467 1101 48-49 0.6265921791220066 1101 50-51 0.7190888729348259 1101 52-53 0.7942868120868667 1101 54-55 0.8865582806867707 1101 56-57 0.8494411673811371 1101 58-59 0.7858545961902621 1101 60-61 0.8033816013993089 1101 62-63 0.6788491816568225 1101 64-65 0.5954746574170571 1101 66-67 0.6314780910756852 1101 68-69 0.7525227896384195 1101 70-71 0.8880707784066004 1101 72-73 0.8018849403766097 1101 74-75 0.6282055097355226 1101 76-77 0.6369196548060643 1101 78-79 0.6871807620994446 1101 80-81 0.6855757867246481 1101 82-83 0.7838686550934355 1101 84-85 0.7410613300026796 1101 86-87 0.6801775630191322 1101 88-89 0.6538818392183217 1101 90-91 0.7259626936737504 1101 92-93 0.5700216032393008 1101 94-95 0.7687060475445726 1101 96-97 0.9655344785755524 1101 98-99 0.9209172139195445 1101 100-101 1.2190543341295275 1102 1 0.30675433918976225 1102 2 0.477188523401491 1102 3 0.40153513502008664 1102 4 0.3270686701253993 1102 5 0.38926637385298335 1102 6 0.3808941947995734 1102 7 0.3241527748461692 1102 8 0.2928998582366873 1102 9 0.45832988611493164 1102 10-11 0.48252584886447636 1102 12-13 0.6212292822525853 1102 14-15 0.6940165494986772 1102 16-17 0.625252671217055 1102 18-19 0.6683460581425109 1102 20-21 0.5480440774390374 1102 22-23 0.6006227445949506 1102 24-25 0.6764253910313798 1102 26-27 0.6396250039545848 1102 28-29 0.5584015589271374 1102 30-31 0.5909537113479502 1102 32-33 0.4027395670912739 1102 34-35 0.3375476511599089 1102 36-37 0.46409199674199186 1102 38-39 0.567524315947864 1102 40-41 0.4723136002127504 1102 42-43 0.3302578132089735 1102 44-45 0.3206511523070432 1102 46-47 0.28424312314564304 1102 48-49 0.3219458284824199 1102 50-51 0.3320606629487983 1102 52-53 0.12063315197721636 1102 54-55 0.2480369367654518 1102 56-57 0.4136686007359742 1102 58-59 0.3804435392731449 1102 60-61 0.32428967540769094 1102 62-63 0.5491203643398279 1102 64-65 0.3701810693271952 1102 66-67 0.3817846118216792 1102 68-69 0.4222336691934032 1102 70-71 0.5435070288096853 1102 72-73 0.3530873414837785 1102 74-75 0.29822710895082594 1102 76-77 0.38058433286690985 1102 78-79 0.35391209204677665 1102 80-81 0.5283617159947767 1102 82-83 0.45474683283180184 1102 84-85 0.6030749277668406 1102 86-87 0.47685941296001033 1102 88-89 0.26408486512394447 1102 90-91 0.39230184502277865 1102 92-93 0.4274365500306594 1102 94-95 0.4041781398803792 1102 96-97 0.5379489550885026 1102 98-99 0.5638961252922208 1102 100-101 0.6797340207897449 1103 1 0.3821325812646883 1103 2 0.3520663023228572 1103 3 0.4409139088639229 1103 4 0.30004280214131995 1103 5 0.3040632724395067 1103 6 0.2334162374783162 1103 7 0.31121878280555393 1103 8 0.41182096774677035 1103 9 0.3728330252668428 1103 10-11 0.40209258586186536 1103 12-13 0.44557936630637585 1103 14-15 0.506545496596253 1103 16-17 0.6174605413968308 1103 18-19 0.4323693187435893 1103 20-21 0.5394939625083381 1103 22-23 0.6449857797946237 1103 24-25 0.6786586060032462 1103 26-27 0.6742628864851241 1103 28-29 0.6597131840815322 1103 30-31 0.5603791436246084 1103 32-33 0.7224441780422808 1103 34-35 0.45326313764268633 1103 36-37 0.48673398761615516 1103 38-39 0.7470295564446374 1103 40-41 0.7090761997050024 1103 42-43 0.5315066124404879 1103 44-45 0.5272219290327271 1103 46-47 0.5864711919668224 1103 48-49 0.5415663856390083 1103 50-51 0.5313228335951621 1103 52-53 0.5900821707778192 1103 54-55 0.5602764128873119 1103 56-57 0.6739598299798288 1103 58-59 0.6398663555898665 1103 60-61 0.5616633802931048 1103 62-63 0.5698925871338503 1103 64-65 0.6265322769572208 1103 66-67 0.5793313989082733 1103 68-69 0.602733832154847 1103 70-71 0.5552884975210972 1103 72-73 0.5963683985031238 1103 74-75 0.4465788397728403 1103 76-77 0.45709901655018115 1103 78-79 0.4342810281658913 1103 80-81 0.44388765259419927 1103 82-83 0.5227714400089667 1103 84-85 0.5841799778560421 1103 86-87 0.5682507605653342 1103 88-89 0.5081882341372541 1103 90-91 0.6858104047867428 1103 92-93 0.7188244668688633 1103 94-95 0.5613523027732015 1103 96-97 0.5469311322533166 1103 98-99 0.8444117267056974 1103 100-101 0.8017899937552997 1104 1 0.2563689742003916 1104 2 0.2686184236748481 1104 3 0.20536653203673083 1104 4 0.23248234161809478 1104 5 0.2824989680955028 1104 6 0.17403155516540636 1104 7 0.28825287271664735 1104 8 0.22903779232598254 1104 9 0.2279667318170624 1104 10-11 0.19377235801029968 1104 12-13 0.26387892494098253 1104 14-15 0.42745740324982506 1104 16-17 0.3724800260143937 1104 18-19 0.20997686709259966 1104 20-21 0.3568172478517866 1104 22-23 0.31728218698636823 1104 24-25 0.42571957406603644 1104 26-27 0.25465761338390536 1104 28-29 0.34496614711181195 1104 30-31 0.30065649306304465 1104 32-33 0.41766692357603574 1104 34-35 0.27850792080994324 1104 36-37 0.3935764787878284 1104 38-39 0.3757401097955224 1104 40-41 0.4499080642031643 1104 42-43 0.3366657980896832 1104 44-45 0.47025903762985166 1104 46-47 0.5895955728604676 1104 48-49 0.6286727361129465 1104 50-51 0.42876971554574794 1104 52-53 0.4350842336146954 1104 54-55 0.5179941289796872 1104 56-57 0.5422773107862611 1104 58-59 0.5393135255206474 1104 60-61 0.6629486706322822 1104 62-63 0.582385515279725 1104 64-65 0.43319467838527714 1104 66-67 0.5384604222060787 1104 68-69 0.4859504586200174 1104 70-71 0.5710766033262473 1104 72-73 0.5058024561440959 1104 74-75 0.3223817632824364 1104 76-77 0.5689913221686567 1104 78-79 0.538734100669501 1104 80-81 0.5774846784327607 1104 82-83 0.5894033686388944 1104 84-85 0.4482096684100867 1104 86-87 0.3824555369517739 1104 88-89 0.4294195230835136 1104 90-91 0.3876166124960321 1104 92-93 0.3267892127825043 1104 94-95 0.07849146460786116 1104 96-97 0.4838739365268623 1104 98-99 0.6747995604614694 1104 100-101 0.8049549676022032 1105 1 0.3209271204616009 1105 2 0.3744272800152437 1105 3 0.34464902890049487 1105 4 0.3128615721918848 1105 5 0.26573887060850154 1105 6 0.21348348051385813 1105 7 0.40928305337267545 1105 8 0.43393977787330584 1105 9 0.41374474335761846 1105 10-11 0.5134038250549509 1105 12-13 0.5184438612183442 1105 14-15 0.40707076358473415 1105 16-17 0.3604434718559659 1105 18-19 0.479410997745191 1105 20-21 0.472421853968946 1105 22-23 0.6642559743543615 1105 24-25 0.6560514330100133 1105 26-27 0.6062286527516321 1105 28-29 0.6230970453502209 1105 30-31 0.6795827295170653 1105 32-33 0.581155010836909 1105 34-35 0.3184707069650443 1105 36-37 0.3622346854212424 1105 38-39 0.4226214321004065 1105 40-41 0.3837789528632456 1105 42-43 0.4199678062208605 1105 44-45 0.45511943123287324 1105 46-47 0.4614769818358653 1105 48-49 0.5079171771896256 1105 50-51 0.3864346334489497 1105 52-53 0.377895805466288 1105 54-55 0.5809857675032006 1105 56-57 0.6650857228783309 1105 58-59 0.7320231976039793 1105 60-61 0.671643704807714 1105 62-63 0.5171317761896859 1105 64-65 0.4974990038385272 1105 66-67 0.5209269760524862 1105 68-69 0.5783939779185729 1105 70-71 0.6321399465994517 1105 72-73 0.6831910089989961 1105 74-75 0.6258724556114998 1105 76-77 0.6682265910280307 1105 78-79 0.7298019854779412 1105 80-81 0.688782750236566 1105 82-83 0.6696057913523177 1105 84-85 0.6537200810063339 1105 86-87 0.631774182851192 1105 88-89 0.7068290749672101 1105 90-91 0.8353315070972513 1105 92-93 0.6995021634877823 1105 94-95 0.5861110414477153 1105 96-97 0.6673586461137582 1105 98-99 0.7651610062446004 1105 100-101 0.7241163832735218 1106 1 0.07042889337357394 1106 2 0.056183109919224705 1106 3 0.025007234765336506 1106 4 -8.144378503018856E-4 1106 5 0.013972429114595286 1106 6 0.04201571645350555 1106 7 0.10550688027434774 1106 8 0.0897748582366873 1106 9 0.14672090610316957 1106 10-11 0.0888496412415094 1106 12-13 0.10471479322652044 1106 14-15 0.13041884961626238 1106 16-17 0.07995456226154118 1106 18-19 0.06141141185601384 1106 20-21 0.12229115023935577 1106 22-23 0.09174775684265057 1106 24-25 0.15373500057478395 1106 26-27 0.09348180387620886 1106 28-29 0.2912195703909859 1106 30-31 0.3007230872052844 1106 32-33 0.30526014322296646 1106 34-35 0.2321868339935378 1106 36-37 0.25336883144706945 1106 38-39 0.3100026987618989 1106 40-41 0.28188180763975623 1106 42-43 0.2628709819332613 1106 44-45 0.41726711350634105 1106 46-47 0.3743907691381949 1106 48-49 0.516904676219049 1106 50-51 0.4246945915202289 1106 52-53 0.4885539828610135 1106 54-55 0.37648619455499244 1106 56-57 0.37774457320315236 1106 58-59 0.41740249723903844 1106 60-61 0.5825063494229781 1106 62-63 0.4637891253627515 1106 64-65 0.3743269149082238 1106 66-67 0.4291755460760456 1106 68-69 0.4460661574356166 1106 70-71 0.6349804880493508 1106 72-73 0.6734335226517132 1106 74-75 0.4781901821430665 1106 76-77 0.47380155802858326 1106 78-79 0.5795315193444424 1106 80-81 0.5557108243624036 1106 82-83 0.5281610622066779 1106 84-85 0.6445578789720159 1106 86-87 0.5335846236204027 1106 88-89 0.39528832387370016 1106 90-91 0.513832684724914 1106 92-93 0.3592842376653813 1106 94-95 0.44637451946102047 1106 96-97 0.5296603258508021 1106 98-99 0.3971911845710743 1106 100-101 0.7517841628630357 1107 1 -0.018313842628419508 1107 2 0.024878458466425002 1107 3 0.13557463718459317 1107 4 -0.0020200744633456225 1107 5 0.10022308380969491 1107 6 0.15118045021083049 1107 7 0.12014411683751547 1107 8 0.19195667641850633 1107 9 0.23529254845259118 1107 10-11 0.2072220068731312 1107 12-13 0.12227284285864215 1107 14-15 0.277429698849339 1107 16-17 0.1293554850698726 1107 18-19 0.14989800619446214 1107 20-21 0.33378136098883004 1107 22-23 0.3303475822572892 1107 24-25 0.3273277178712064 1107 26-27 0.24165638923597754 1107 28-29 0.46677737061545344 1107 30-31 0.3314650749843082 1107 32-33 0.41605774890945213 1107 34-35 0.2712089065711609 1107 36-37 0.2622529815904855 1107 38-39 0.3333714479824934 1107 40-41 0.5881883296499879 1107 42-43 0.27065175260291596 1107 44-45 0.3472020181079145 1107 46-47 0.3040740214140385 1107 48-49 0.2683397678763626 1107 50-51 0.29033095515659113 1107 52-53 0.3271612905053587 1107 54-55 0.37213677442779414 1107 56-57 0.2074919232467991 1107 58-59 0.3756905630826637 1107 60-61 0.38862382042933774 1107 62-63 0.3186623015692618 1107 64-65 0.00262371001983297 1107 66-67 0.06766962264419618 1107 68-69 0.18128426443149692 1107 70-71 0.24934063270579543 1107 72-73 0.22658977654871393 1107 74-75 0.09450443579065393 1107 76-77 0.06272935451193007 1107 78-79 0.14727762234980446 1107 80-81 0.19822535235841343 1107 82-83 0.25406853196599855 1107 84-85 0.23857059876251085 1107 86-87 0.07984101469161331 1107 88-89 0.36734568763476716 1107 90-91 0.4940718666677988 1107 92-93 0.12749174483585435 1107 94-95 0.26494491477215476 1107 96-97 0.46138672565127337 1107 98-99 0.4055246426082384 1107 100-101 0.36613607706680185 1108 1 -0.001867923583962039 1108 2 0.03396671327730516 1108 3 0.02613867169023365 1108 4 0.04967468333360614 1108 5 0.06927948726026045 1108 6 -0.08423826246335864 1108 7 -0.1180297385042266 1108 8 -0.12422646890332345 1108 9 -0.014586681806200374 1108 10-11 -0.13025908138168063 1108 12-13 -0.16076896205177604 1108 14-15 -0.10554309478039414 1108 16-17 0.06864679230277915 1108 18-19 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-1.7372601947893074 2316 50-51 -1.6320302544756657 2316 52-53 -1.7860599153934658 2316 54-55 -1.7950773379485412 2316 56-57 -1.7164686890361978 2316 58-59 -1.801262208991261 2316 60-61 -1.800188574567862 2316 62-63 -1.7432956973107068 2316 64-65 -1.6253790900548353 2316 66-67 -1.6870535433068952 2316 68-69 -1.7303625528169633 2316 70-71 -1.4462374893774594 2316 72-73 -1.4960917616256424 2316 74-75 -1.4144505763430075 2316 76-77 -1.8127580451520622 2316 78-79 -1.9426688028881998 2316 80-81 -1.6142204195288379 2316 82-83 -1.6921338200967213 2316 84-85 -1.8017297559136125 2316 86-87 -1.8225838499714477 2316 88-89 -1.7712596885085965 2316 90-91 -2.067756395419188 2316 92-93 -1.9891720595322617 2316 94-95 -2.1086173402213113 2316 96-97 -2.3336883163498463 2316 98-99 -1.906040225788253 2316 100-101 -1.7245282873162502 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13192.0 3 3014.0 4 2495.0 5 2103.0 6 1997.0 7 2101.0 8 2618.0 9 3085.0 10 3556.0 11 4476.0 12 5833.0 13 8286.0 14 8711.0 15 8151.0 16 9927.0 17 12915.0 18 12037.0 19 8080.0 20 7544.0 21 7480.0 22 7964.0 23 8578.0 24 9548.0 25 11089.0 26 12955.0 27 15541.0 28 18571.0 29 22815.0 30 27916.0 31 34427.0 32 43045.0 33 57140.0 34 78632.0 35 117955.0 36 190626.0 37 325268.0 38 358207.0 39 74144.0 40 86.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.89349477879964 18.160798438829953 12.817242417322344 23.12846436504806 2 15.589828798084469 19.516032587900035 40.9384521396024 23.955686474413103 3 20.237659797451542 24.584228302826546 28.214107832150415 26.964004067571494 4 12.280766604345855 15.309130031303075 37.04943058492719 35.360672779423886 5 13.645469420101596 37.9625636439454 33.51826907924048 14.87369785671252 6 32.9636016158037 35.27193893201056 17.439149582462992 14.325309869722746 7 30.050835356090676 32.07510585024873 19.77961618300988 18.094442610650717 8 27.224271524810828 35.10377512665468 19.0835466942674 18.58840665426709 9 27.49259839353523 16.062766919926457 19.698098768074352 36.74653591846397 10-11 25.839124526569346 26.43747667499855 26.688892224956284 21.034506573475824 12-13 26.412199879051805 23.51780905425321 27.570059971497727 22.499931095197255 14-15 23.10575207006039 24.886866530392016 26.019595534065203 25.987785865482394 16-17 22.611988167361403 28.418179094158464 26.39251101121584 22.577321727264295 18-19 22.302532698415785 26.831070750503024 29.031750741358653 21.834645809722538 20-21 23.27842721716098 25.964645264294788 29.017386846720594 21.739540671823633 22-23 22.6120107408648 26.17785852629913 28.805907281083215 22.40422345175285 24-25 22.34210738815261 26.21196854714248 29.180804637697705 22.265119427007207 26-27 22.418930324842908 26.505334173372553 28.953045461015837 22.12269004076871 28-29 22.049009545397798 26.576709359094718 29.175677801072418 22.198603294435067 30-31 22.315015986873252 26.345005156009833 29.207822866741466 22.13215599037545 32-33 22.098681080856718 26.426894237750474 29.098003024178187 22.376421657214625 34-35 22.587616710396325 26.47715595444487 28.91440029361883 22.020827041539977 36-37 22.539203323703045 26.343454309670015 28.758083919332318 22.35925844729462 38-39 22.34972131132055 26.590492955337826 28.916226616194006 22.143559117147618 40-41 22.550838056172324 26.461006644826764 28.90436108500133 22.083794213999585 42-43 22.411909234308634 26.4982157388965 28.68751095413175 22.402364072663115 44-45 22.431739051954626 26.74484829488547 28.549696368213244 22.273716284946655 46-47 22.346930662614014 26.871853958505486 28.149397706566702 22.631817672313794 48-49 22.729522665588277 26.920646734474484 27.9450307631296 22.404799836807634 50-51 22.589441183860746 27.103907518700694 27.809242980782923 22.497408316655633 52-53 22.69520070203268 27.247529411304665 27.62363696855093 22.43363291811172 54-55 22.542804732224024 26.923109505175667 27.817781354568172 22.71630440803214 56-57 22.56407633251534 27.43191624952595 27.595992321396224 22.408015096562483 58-59 22.725338667743973 27.000925268295227 27.736709042216994 22.537027021743803 60-61 22.791499576308045 27.01890601140857 27.9422319394992 22.247362472784186 62-63 22.65353812793875 27.06943402397813 27.934773437253117 22.34225441083 64-65 22.46558568844263 26.988777063555368 27.87741701726124 22.668220230740758 66-67 22.52774386390555 27.30091326402308 27.579654890698883 22.591687981372488 68-69 22.503298263723597 27.166191110637566 27.71681949952412 22.61369112611472 70-71 22.42041528240285 27.099883959653663 27.834371789099766 22.64532896884372 72-73 22.603849453194155 27.124287028189165 27.6777228950166 22.59414062360008 74-75 22.527895407049414 27.205043786763223 27.5260967544748 22.740964051712563 76-77 22.61876409300889 27.217014987907827 27.5338256648577 22.630395254225583 78-79 22.65641468401033 27.11218264602152 27.541690762626963 22.689711907341188 80-81 22.322072045655066 27.61002920558464 27.457941221317338 22.609957527442955 82-83 22.65509999420059 27.512235776622383 27.28941464409545 22.543249585081572 84-85 22.637993714392582 27.32828451659274 27.2225578108414 22.811163958173278 86-87 22.521624330287615 27.151127254149905 27.605182801668693 22.72206561389379 88-89 22.727326050105805 27.261790167186632 27.394415902575155 22.616467880132404 90-91 22.589939688193436 27.436298424800594 27.51788102955096 22.455880857455014 92-93 22.557514483928273 27.334334723525377 27.509605165687272 22.59854562685907 94-95 22.41610016665119 27.440788326923045 27.420327535566102 22.722783970859663 96-97 22.560429226739767 27.427209527832996 27.317603227799676 22.69475801762756 98-99 22.512069616355078 27.18612076358339 27.254224569286585 23.047585050774956 100-101 22.501618856650733 27.354149810362504 27.144708195388507 22.999523137598253 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 189.0 1 150.0 2 247.5 3 403.0 4 322.5 5 299.0 6 616.0 7 1254.5 8 1963.5 9 2177.0 10 1879.0 11 1665.0 12 1755.5 13 1936.0 14 2147.5 15 2351.0 16 2526.5 17 2617.0 18 2593.0 19 2703.5 20 2874.5 21 3099.5 22 3484.5 23 4088.0 24 4874.5 25 5843.0 26 7407.5 27 9310.0 28 11027.0 29 12984.5 30 15597.5 31 18551.5 32 21812.5 33 25243.0 34 28967.5 35 32418.5 36 36114.0 37 39634.0 38 41808.5 39 43705.0 40 45013.5 41 47421.0 42 50464.0 43 52508.5 44 54634.0 45 56511.0 46 57157.5 47 57719.5 48 61772.5 49 62968.5 50 59576.0 51 56857.5 52 54406.5 53 52893.0 54 50880.0 55 47169.0 56 42651.0 57 38401.5 58 34870.5 59 30867.0 60 26580.5 61 22254.0 62 18169.0 63 14230.0 64 10898.0 65 8718.5 66 6602.0 67 5206.5 68 3797.0 69 2430.0 70 1951.0 71 1649.5 72 1397.0 73 1345.5 74 1122.5 75 860.5 76 586.5 77 317.5 78 186.0 79 110.5 80 72.5 81 50.5 82 39.0 83 25.0 84 15.5 85 14.0 86 12.0 87 14.5 88 16.5 89 17.0 90 16.0 91 13.0 92 12.0 93 10.5 94 12.5 95 17.5 96 15.0 97 10.0 98 7.5 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04539241090766665 2 0.012061412041179996 3 0.010116023002279996 4 0.005641628212809998 5 0.00849486546986333 6 0.005771320815403331 7 0.004604087392063331 8 0.007781556155599997 9 0.013812262176189995 10-11 0.0025614289012183323 12-13 0.008138210812731663 14-15 0.008040941360786664 16-17 0.01757334765139666 18-19 0.011380525877564995 20-21 0.003339584516778332 22-23 0.009499983139961662 24-25 0.02000508395002166 26-27 0.007554594101061663 28-29 0.017216692994264992 30-31 0.014136493682673326 32-33 0.01306652971127833 34-35 0.2632435601138182 36-37 0.4664718683775715 38-39 0.4637483237231115 40-41 0.48346159931729815 42-43 0.47331315316436984 44-45 0.4875793394496365 46-47 0.4954257419065331 48-49 0.5007755617635081 50-51 0.5105673532593048 52-53 0.4997380209427615 54-55 0.48783872465482314 56-57 0.4833643298653531 58-59 0.4810298630186731 60-61 0.47885751192523485 62-63 0.4756151968604015 64-65 0.49101619341835984 66-67 0.4652073655022865 68-69 0.4920861573897548 70-71 0.4731834605617765 72-73 0.48132167137450815 74-75 0.4945178936883798 76-77 0.48229436589395813 78-79 0.4828131363043314 80-81 0.4844018706860998 82-83 0.4843370243848032 84-85 0.4857636430133298 86-87 0.4859581819172198 88-89 0.5002892145037832 90-91 0.5239581144770664 92-93 0.5127721275033915 94-95 0.4841100623302648 96-97 0.4583660807154882 98-99 0.45077906346377816 100-101 0.45872273537261976 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1542108.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.79530306455867 #Duplication Level Percentage of deduplicated Percentage of total 1 77.07585837719854 44.54622593871193 2 13.068895609116584 15.106415648959459 3 4.37062762197383 7.578052439829265 4 1.9174608543107916 4.432809247572791 5 0.9792431028884663 2.829782595265886 6 0.6075031713922667 2.1066497941977955 7 0.3766988673353042 1.5239997641203922 8 0.26806982806542823 1.2394541564404449 9 0.18292223947622124 0.9514841640998386 >10 1.0245826731404644 10.868156081921347 >50 0.08691111397793672 3.446660919650393 >100 0.03942437990646348 3.896365233179222 >500 0.001238985837124552 0.4557495829155592 >1k 4.5054030440892795E-4 0.6168014995179151 >5k 1.1263507610223199E-4 0.40139293361767436 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6166 0.39984229379524655 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4324 0.28039540680678654 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2925 0.18967543129274994 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.484630129666663E-5 2 6.484630129666663E-5 0.0 0.0 0.0 6.484630129666663E-5 3 6.484630129666663E-5 0.0 0.0 0.0 6.484630129666663E-5 4 6.484630129666663E-5 0.0 0.0 0.0 6.484630129666663E-5 5 6.484630129666663E-5 0.0 0.0 0.0 6.484630129666663E-5 6 6.484630129666663E-5 0.0 0.0 0.0 6.484630129666663E-5 7 1.2969260259333327E-4 0.0 0.0 0.0 6.484630129666663E-5 8 1.2969260259333327E-4 0.0 0.0 0.0 6.484630129666663E-5 9 1.2969260259333327E-4 0.0 0.0 0.0 6.484630129666663E-5 10-11 1.2969260259333327E-4 0.0 0.0 3.242315064833332E-5 6.484630129666663E-5 12-13 1.2969260259333327E-4 0.0 0.0 6.484630129666663E-5 6.484630129666663E-5 14-15 1.9453890388999992E-4 0.0 0.0 9.726945194499996E-5 3.242315064833332E-4 16-17 1.9453890388999992E-4 0.0 0.0 1.2969260259333327E-4 3.242315064833332E-4 18-19 1.9453890388999992E-4 0.0 0.0 1.6211575324166658E-4 3.242315064833332E-4 20-21 1.9453890388999992E-4 0.0 0.0 3.8907780777999983E-4 3.8907780777999983E-4 22-23 1.9453890388999992E-4 0.0 0.0 9.726945194499996E-4 4.863472597249998E-4 24-25 3.242315064833332E-4 0.0 0.0 0.001718426984361666 5.187704103733331E-4 26-27 3.242315064833332E-4 0.0 0.0 0.0019453890388999992 5.187704103733331E-4 28-29 4.5392410907666645E-4 0.0 0.0 0.002658698353163332 5.836167116699997E-4 30-31 5.187704103733331E-4 0.0 0.0 0.003955624379096665 5.836167116699997E-4 32-33 5.187704103733331E-4 0.0 0.0 0.007035823690688331 6.484630129666664E-4 34-35 5.187704103733331E-4 0.0 0.0 0.010505100810059995 7.133093142633331E-4 36-37 5.187704103733331E-4 0.0 0.0 0.01793000230852833 7.133093142633331E-4 38-39 5.836167116699997E-4 0.0 0.0 0.030088683801653318 7.133093142633331E-4 40-41 6.160398623183331E-4 0.0 0.0 0.04464667844275498 7.457324649116664E-4 42-43 6.484630129666664E-4 0.0 0.0 0.05862105637218665 8.430019168566663E-4 44-45 7.133093142633331E-4 0.0 0.0 0.0745408233405183 8.430019168566663E-4 46-47 7.781556155599997E-4 0.0 0.0 0.09392986742822163 8.430019168566663E-4 48-49 7.781556155599997E-4 0.0 0.0 0.11776088315474662 8.430019168566663E-4 50-51 7.781556155599997E-4 0.0 0.0 0.14415332778248996 8.430019168566663E-4 52-53 7.781556155599997E-4 0.0 0.0 0.17138877432708993 9.402713688016663E-4 54-55 7.781556155599997E-4 0.0 0.0 0.20034264785605158 0.001102387122043333 56-57 7.781556155599997E-4 0.0 0.0 0.23879650452497492 0.0011672334233399995 58-59 7.781556155599997E-4 0.0 0.0 0.27977936694446826 0.0011672334233399995 60-61 7.781556155599997E-4 0.0 0.0 0.3289004401766932 0.0011672334233399995 62-63 7.781556155599997E-4 0.0 0.0 0.37682185683492986 0.0011672334233399995 64-65 7.781556155599997E-4 0.0 0.0 0.4248729660957598 0.0011672334233399995 66-67 8.430019168566663E-4 0.0 0.0 0.4818404417848815 0.001199656573988333 68-69 8.430019168566663E-4 0.0 0.0 0.5534307584164014 0.0012969260259333328 70-71 8.754250675049996E-4 0.0 0.0 0.6317650903827747 0.0012969260259333328 72-73 9.402713688016663E-4 0.0 0.0 0.7133093142633331 0.0013617723272299994 74-75 9.726945194499996E-4 0.0 0.0 0.791708492531003 0.0013617723272299994 76-77 9.726945194499996E-4 0.0 0.0 0.8826554300995779 0.0013617723272299994 78-79 9.726945194499996E-4 0.0 0.0 0.9887439790209247 0.0013617723272299994 80-81 0.001102387122043333 0.0 0.0 1.1186959668194445 0.0013617723272299994 82-83 0.001102387122043333 0.0 0.0 1.2461837951686912 0.0013617723272299994 84-85 0.001102387122043333 0.0 0.0 1.3683542268116113 0.0013617723272299994 86-87 0.001134810272691666 0.0 0.0 1.5044990363839625 0.0013617723272299994 88-89 0.0011672334233399995 0.0 0.0 1.6612649697686543 0.0013941954778783328 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6885 0.0 35.8199 1 GTACCTG 1580 0.0 35.052532 1 TATCACG 95 8.9594687E-7 34.870033 2 TACCTGG 1555 0.0 34.69384 2 GGTATCA 4240 0.0 33.715664 1 GTACATG 8430 0.0 31.38881 1 TACATGG 8375 0.0 31.247711 2 ACATGGG 8380 0.0 30.15708 3 ATCAACG 8270 0.0 29.413698 3 TCAACGC 8335 0.0 29.184319 4 CAACGCA 8440 0.0 28.821241 5 AACGCAG 8670 0.0 28.056664 6 TATCAAC 8760 0.0 27.929575 2 GTGTAGC 1055 0.0 24.2288 1 CATGGGA 4875 0.0 24.075155 4 ACGCAGA 10165 0.0 23.790607 7 ATAACGC 120 1.7545068E-4 23.662577 3 ACCTGGG 2200 0.0 23.662575 3 CGCAGAG 10315 0.0 23.490526 8 CATGGGG 3570 0.0 23.331167 4 >>END_MODULE