FastQCFastQC Report
Thu 26 May 2016
SRR936304_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936304_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1542108
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61410.39822113626282984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46940.3043885382865532No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33700.2185320353697666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70250.038.1254231
GGTATCA41100.036.2288971
ATCAACG82750.031.8674893
TCAACGC83900.031.430694
CAACGCA85250.030.8773235
AACGCAG87350.030.297896
TATCAAC89550.029.4514452
GTACATG78800.027.5894221
GTACCTG14250.027.3748421
TACATGG79600.026.9949512
ACGCAGA101200.026.010797
ACATGGG79900.025.9403463
CGCAGAG102400.025.7059788
TACCTGG15450.025.1757722
GCAGAGT111300.023.4799699
GTGTAGC11150.022.1861081
CATGGGG32950.022.0230314
GAGTACT57350.021.95767212-13
AGTACAT56300.021.6532042
AGTACTT59250.021.29356812-13
Produced by FastQC (version 0.11.5)