##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936304_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1542108 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.47131848093648 34.0 31.0 34.0 31.0 34.0 2 32.6001401977034 34.0 31.0 34.0 31.0 34.0 3 32.68572499461776 34.0 31.0 34.0 31.0 34.0 4 36.17135051500932 37.0 35.0 37.0 35.0 37.0 5 36.142412204592674 37.0 35.0 37.0 35.0 37.0 6 36.18640977155945 37.0 37.0 37.0 35.0 37.0 7 36.14042466545793 37.0 36.0 37.0 35.0 37.0 8 36.1208942564334 37.0 36.0 37.0 35.0 37.0 9 37.88256788759283 39.0 38.0 39.0 35.0 39.0 10-11 37.81806656861906 39.0 38.0 39.0 35.0 39.0 12-13 37.76654715493338 39.0 38.0 39.0 35.0 39.0 14-15 39.09284790689108 40.0 38.0 41.0 36.0 41.0 16-17 39.02214533612432 40.0 38.0 41.0 35.5 41.0 18-19 39.06475065300226 40.0 38.5 41.0 36.0 41.0 20-21 39.033576442116896 40.0 39.0 41.0 35.0 41.0 22-23 38.911596334368284 40.0 38.0 41.0 35.0 41.0 24-25 39.06685783356289 40.0 39.0 41.0 35.0 41.0 26-27 38.98554770483001 40.0 38.5 41.0 35.0 41.0 28-29 38.88752538732696 40.0 38.0 41.0 35.0 41.0 30-31 38.7558611329427 40.0 38.0 41.0 35.0 41.0 32-33 38.58398374173534 40.0 38.0 41.0 34.5 41.0 34-35 38.43791323305501 40.0 38.0 41.0 34.0 41.0 36-37 38.28590183048139 40.0 38.0 41.0 34.0 41.0 38-39 38.14963057062151 40.0 38.0 41.0 33.5 41.0 40-41 37.999353158144565 40.0 38.0 41.0 33.0 41.0 42-43 37.997252786445564 40.0 38.0 41.0 33.0 41.0 44-45 37.835027118723204 40.0 38.0 41.0 33.0 41.0 46-47 37.5860773045727 40.0 37.0 41.0 33.0 41.0 48-49 37.31981125835544 40.0 37.0 41.0 32.5 41.0 50-51 37.110582397601206 40.0 37.0 41.0 31.5 41.0 52-53 36.9050909534222 40.0 36.0 41.0 31.0 41.0 54-55 36.717895244691036 40.0 36.0 41.0 31.0 41.0 56-57 36.48654179862889 39.0 35.5 41.0 31.0 41.0 58-59 36.24074351472141 39.0 35.0 41.0 30.0 41.0 60-61 35.98054221883292 39.0 35.0 41.0 30.0 41.0 62-63 35.699287922765464 38.0 35.0 40.0 29.0 41.0 64-65 35.34156038357884 38.0 35.0 40.0 29.0 41.0 66-67 35.00606442609727 37.0 34.5 40.0 28.0 41.0 68-69 34.65730837269504 37.0 34.0 39.5 28.0 41.0 70-71 34.28498944302215 36.0 34.0 39.0 28.0 41.0 72-73 33.86982850747159 35.5 34.0 39.0 26.5 40.0 74-75 33.438027686776806 35.0 33.5 37.5 26.0 39.5 76-77 32.70128421615088 35.0 32.5 37.0 26.0 39.0 78-79 32.738444389108935 35.0 33.0 37.0 26.0 39.0 80-81 32.546078484775386 35.0 33.0 36.0 26.0 38.5 82-83 32.251152967237054 35.0 33.0 36.0 26.0 37.0 84-85 31.942843173111093 35.0 33.0 35.5 25.5 37.0 86-87 31.525932035888538 35.0 32.0 35.0 24.5 36.5 88-89 31.025750790476415 35.0 32.0 35.0 21.5 36.0 90-91 30.798192798429163 34.5 32.0 35.0 20.0 36.0 92-93 30.51246216218319 34.0 31.0 35.0 18.5 35.5 94-95 30.34994598303102 34.0 31.0 35.0 18.0 35.0 96-97 30.104925854739097 34.0 31.0 35.0 8.5 35.0 98-99 29.654286535054613 34.0 31.0 35.0 2.0 35.0 100-101 26.318606089845847 31.0 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09994050429392587 1101 2 0.07760739428491803 1101 3 0.22451832064174937 1101 4 0.10542696301138932 1101 5 0.1381086998499086 1101 6 0.1547581347675404 1101 7 0.16386801967398412 1101 8 0.07227504734230195 1101 9 0.16941971098685116 1101 10-11 0.15680918331346305 1101 12-13 0.2239593048247599 1101 14-15 0.3181894758976753 1101 16-17 0.4060385341276245 1101 18-19 0.36422961816974464 1101 20-21 0.12945473504425564 1101 22-23 0.22568587504473925 1101 24-25 0.17805061273448786 1101 26-27 0.18871770166723678 1101 28-29 0.3189472827209059 1101 30-31 0.19211582641865732 1101 32-33 0.23659617489376217 1101 34-35 0.3282885541739091 1101 36-37 0.39138987586500207 1101 38-39 0.41502202580380043 1101 40-41 0.5485829605514922 1101 42-43 0.4700987772128187 1101 44-45 0.38672428925328006 1101 46-47 0.37744217660252133 1101 48-49 0.44939923581930685 1101 50-51 0.3449776662108732 1101 52-53 0.4026032936329571 1101 54-55 0.40245901608598444 1101 56-57 0.16532302525795473 1101 58-59 0.0424178426637809 1101 60-61 0.23683946596963779 1101 62-63 0.18066023516010432 1101 64-65 0.3089583807132854 1101 66-67 0.2914957681319805 1101 68-69 0.25392503291423196 1101 70-71 0.33724877763497574 1101 72-73 0.33442170923904513 1101 74-75 0.31821750473169885 1101 76-77 0.17293444535068403 1101 78-79 0.06263058128546817 1101 80-81 0.09596193099645944 1101 82-83 0.3006420447626965 1101 84-85 0.3475123606589392 1101 86-87 0.18105795524663648 1101 88-89 0.3511529540939371 1101 90-91 0.6251694719005165 1101 92-93 0.5761007951426649 1101 94-95 0.487036113072957 1101 96-97 0.4219157292562663 1101 98-99 0.5790165757906607 1101 100-101 0.9474110571784387 1102 1 -0.05837032285860744 1102 2 0.05870388197705978 1102 3 0.0966462632409062 1102 4 0.04045464225464457 1102 5 0.05596449905446832 1102 6 0.12480307994651696 1102 7 0.051735172742887414 1102 8 0.12431471196661192 1102 9 0.0756159648834398 1102 10-11 0.1740692405530595 1102 12-13 0.19965598869017498 1102 14-15 0.17009376752402972 1102 16-17 0.28905846049546113 1102 18-19 0.4265692196423885 1102 20-21 0.33759911723279146 1102 22-23 0.31543598925521366 1102 24-25 0.20750263761892995 1102 26-27 0.28430094766314795 1102 28-29 0.21438003962727947 1102 30-31 0.1423586916234285 1102 32-33 0.12498903203661627 1102 34-35 0.11972763968395128 1102 36-37 0.06509812186091324 1102 38-39 0.0687971856984646 1102 40-41 -0.10230670541647413 1102 42-43 -0.0479162448230781 1102 44-45 -0.03738621273532772 1102 46-47 -0.03990807331685886 1102 48-49 -0.07803479083876397 1102 50-51 -0.25558297283030385 1102 52-53 -0.31835197617247957 1102 54-55 -0.3012361964798558 1102 56-57 -0.19761685300711918 1102 58-59 -0.27108317851218544 1102 60-61 -0.27964463190201627 1102 62-63 -0.11849477532038577 1102 64-65 -0.2407500466757213 1102 66-67 -0.23044355923553184 1102 68-69 -0.32844674903782334 1102 70-71 -0.29841646475995276 1102 72-73 -0.25308534493702695 1102 74-75 -0.1046568368785401 1102 76-77 -0.31195282219741927 1102 78-79 -0.287049125529542 1102 80-81 -0.15310926915402945 1102 82-83 -0.07463256423440612 1102 84-85 -0.010782678575715465 1102 86-87 -0.14796813176830526 1102 88-89 -0.09361032791186474 1102 90-91 -0.010193448662739968 1102 92-93 0.016704632614448656 1102 94-95 0.19416771712541347 1102 96-97 0.032813255591861434 1102 98-99 0.15799647474798917 1102 100-101 0.614842766035796 1103 1 0.10246618393431106 1103 2 0.14393914683228104 1103 3 0.2111781350674491 1103 4 0.03121730178531834 1103 5 0.10330050838615534 1103 6 0.0714096224194094 1103 7 0.2016832823902064 1103 8 0.10179172582691365 1103 9 0.04966650008311291 1103 10-11 0.08208072504599073 1103 12-13 0.09273184295118853 1103 14-15 0.1728533908258001 1103 16-17 0.2655941576273406 1103 18-19 0.1236571416267509 1103 20-21 0.3136534348532152 1103 22-23 0.34863616593972324 1103 24-25 0.17451688387939868 1103 26-27 0.22658365145071713 1103 28-29 0.16338704696912032 1103 30-31 0.1917295747028902 1103 32-33 0.11435794242498076 1103 34-35 0.23323349771880686 1103 36-37 0.29957282853033007 1103 38-39 0.4303560833929865 1103 40-41 0.34603987925485313 1103 42-43 0.35852244736849315 1103 44-45 0.331043868230779 1103 46-47 0.28831127277892676 1103 48-49 0.2853306201671373 1103 50-51 0.25731885576857394 1103 52-53 0.2285701968754168 1103 54-55 0.26813069833282555 1103 56-57 0.18656795384068658 1103 58-59 0.4234756248362501 1103 60-61 0.42386525728420565 1103 62-63 0.4577593844847456 1103 64-65 0.2779219033723024 1103 66-67 0.33281657010438437 1103 68-69 0.36745755829029747 1103 70-71 0.24055276125898928 1103 72-73 0.1411719797507729 1103 74-75 0.20141905169014507 1103 76-77 0.2472321990733306 1103 78-79 0.18901376900867461 1103 80-81 0.2707770180698006 1103 82-83 0.27404380292629327 1103 84-85 0.1887292948015329 1103 86-87 0.1182840553457325 1103 88-89 0.2343206193229328 1103 90-91 0.39441836003768316 1103 92-93 0.5165245173406738 1103 94-95 0.43870314837283786 1103 96-97 0.0712303425274996 1103 98-99 0.19709006607500967 1103 100-101 0.5896620074931853 1104 1 0.0354888322492215 1104 2 0.08264787222375958 1104 3 0.03474635267427573 1104 4 0.09364007305347144 1104 5 0.14656851174387953 1104 6 0.06988751432267293 1104 7 0.09885801284278273 1104 8 0.049793939802327714 1104 9 -0.021127099812318306 1104 10-11 0.07607194675602358 1104 12-13 0.13260944115550188 1104 14-15 0.2350885154231861 1104 16-17 0.18864472572444413 1104 18-19 0.24359757465909837 1104 20-21 0.11028044126843639 1104 22-23 0.11761503105265803 1104 24-25 0.22134608915617093 1104 26-27 0.21983220082706367 1104 28-29 0.23401240311484628 1104 30-31 0.02126138221774454 1104 32-33 0.13253286282193955 1104 34-35 0.2421519482628085 1104 36-37 0.2159133048618287 1104 38-39 0.2759364043377275 1104 40-41 0.36442287045002075 1104 42-43 0.26816636291881224 1104 44-45 0.37420975095135134 1104 46-47 0.21279127449455615 1104 48-49 0.28982214109063165 1104 50-51 0.2621481406994448 1104 52-53 0.2045858887325167 1104 54-55 0.11957019083410358 1104 56-57 0.11534059653895667 1104 58-59 0.22402329781328945 1104 60-61 0.13744520855224351 1104 62-63 0.21792574921473573 1104 64-65 0.16146174165405824 1104 66-67 0.11181755454732212 1104 68-69 0.1455371592845367 1104 70-71 0.026103252765615537 1104 72-73 -0.12766085285104367 1104 74-75 -0.041817243209742117 1104 76-77 -0.007148520958857318 1104 78-79 -0.011737532518843352 1104 80-81 0.029832054965986288 1104 82-83 -0.2027250286297999 1104 84-85 0.0453399210665566 1104 86-87 0.01801486534620267 1104 88-89 0.18637753319643124 1104 90-91 0.35540444712207986 1104 92-93 0.36809549016228615 1104 94-95 0.33133376577378826 1104 96-97 0.18080042485715708 1104 98-99 0.4041154042474986 1104 100-101 0.5532373633325456 1105 1 0.11556991623493218 1105 2 0.15534116110254814 1105 3 0.1593552829074767 1105 4 0.16594755430416086 1105 5 0.09203443017156587 1105 6 0.14024713754859164 1105 7 0.21208289411654846 1105 8 0.14369426672352859 1105 9 0.17201146752894658 1105 10-11 0.20107411351902016 1105 12-13 0.27978539370165834 1105 14-15 0.32554356536769546 1105 16-17 0.22734930356224936 1105 18-19 0.21072443702284716 1105 20-21 0.18240591442756227 1105 22-23 0.15639464079759335 1105 24-25 0.19822388025644244 1105 26-27 0.3622707427614813 1105 28-29 0.2441318053755026 1105 30-31 0.28558863821422875 1105 32-33 0.26350043764707465 1105 34-35 0.4556261434775095 1105 36-37 0.4122524906839189 1105 38-39 0.4157082181932239 1105 40-41 0.51754813510464 1105 42-43 0.5668542703012065 1105 44-45 0.5430270846591014 1105 46-47 0.5811127537770844 1105 48-49 0.5948743885562706 1105 50-51 0.5397758546629632 1105 52-53 0.379294836745558 1105 54-55 0.5642261113468336 1105 56-57 0.5538065085282682 1105 58-59 0.47529797352934366 1105 60-61 0.5530858200796089 1105 62-63 0.5252881374778582 1105 64-65 0.5048387024507619 1105 66-67 0.5500506942834846 1105 68-69 0.5046502672844255 1105 70-71 0.36487157057997166 1105 72-73 0.3870810596751255 1105 74-75 0.4819958535477369 1105 76-77 0.5138277258708612 1105 78-79 0.37262864101732873 1105 80-81 0.34941300547910004 1105 82-83 0.3835143672321024 1105 84-85 0.41290018555525876 1105 86-87 0.46104732320823416 1105 88-89 0.5407542211048053 1105 90-91 0.6963023834227151 1105 92-93 0.662450614445337 1105 94-95 0.5714645936852598 1105 96-97 0.5057834250537745 1105 98-99 0.3113108403265059 1105 100-101 0.4091104796726093 1106 1 0.055070912689110685 1106 2 0.05874981084243558 1106 3 7.896592824536697E-4 1106 4 0.0017396706693020292 1106 5 0.07647939225454792 1106 6 -0.020984324521442943 1106 7 -0.025073199783079758 1106 8 -0.017783073649688674 1106 9 -0.12291308665486866 1106 10-11 0.049949543806050656 1106 12-13 0.033007693569665264 1106 14-15 0.1270033060307938 1106 16-17 0.008025857124899005 1106 18-19 0.0478688840554824 1106 20-21 0.09709261370545619 1106 22-23 0.00419597112862391 1106 24-25 0.11996987502829626 1106 26-27 -0.0826201649176852 1106 28-29 0.028185950366065526 1106 30-31 0.03257339375943502 1106 32-33 0.28357834224629386 1106 34-35 0.265853386793502 1106 36-37 0.11662265399887417 1106 38-39 0.17023108487541805 1106 40-41 0.06686614633739651 1106 42-43 0.051357466719146316 1106 44-45 0.0855316787010807 1106 46-47 0.07874455748855524 1106 48-49 0.15873157208501 1106 50-51 0.21382534090671612 1106 52-53 0.05322785431938826 1106 54-55 0.016182785001625177 1106 56-57 0.19158894505284252 1106 58-59 0.10012003528362357 1106 60-61 0.212059084049379 1106 62-63 0.025068141101321828 1106 64-65 0.11530276539560447 1106 66-67 0.20570460116031342 1106 68-69 0.20530637412502273 1106 70-71 0.19165677647070112 1106 72-73 -0.025826257145531883 1106 74-75 0.23141998067976743 1106 76-77 0.1201765624782567 1106 78-79 -0.0024845924117684604 1106 80-81 0.05730311081853756 1106 82-83 0.1892058029025776 1106 84-85 0.14235640627633472 1106 86-87 0.14394195788484154 1106 88-89 0.19953984110635758 1106 90-91 0.4469947249606179 1106 92-93 0.3917903664964797 1106 94-95 0.3016519788342116 1106 96-97 0.36553573501572956 1106 98-99 0.20904487764824253 1106 100-101 0.5137640500843936 1107 1 -0.005436881300163066 1107 2 0.04639814604632164 1107 3 0.021969856314505876 1107 4 0.023785811085815567 1107 5 0.04029133888130332 1107 6 0.0848458288642675 1107 7 -8.108229281091894E-4 1107 8 0.08218051283241579 1107 9 0.11466466618214355 1107 10-11 0.0488860695573905 1107 12-13 0.12941248219667045 1107 14-15 0.14287461167987203 1107 16-17 0.07650272456472607 1107 18-19 0.10852457678524985 1107 20-21 0.0713125912587671 1107 22-23 0.13384156284828208 1107 24-25 0.18786113328992116 1107 26-27 0.09758260350730552 1107 28-29 0.12051018031992555 1107 30-31 0.06442200331174774 1107 32-33 0.2236264779240642 1107 34-35 0.12123061587443118 1107 36-37 0.17080023225052798 1107 38-39 0.30770059478937384 1107 40-41 0.21044464739737379 1107 42-43 0.003104317947482116 1107 44-45 0.06436270501359331 1107 46-47 0.17255675352296862 1107 48-49 -0.07887137092533436 1107 50-51 0.03595652933420013 1107 52-53 0.186102028156526 1107 54-55 0.2777922126110539 1107 56-57 0.04312205391928359 1107 58-59 0.013453618024612979 1107 60-61 0.12798550879062276 1107 62-63 0.12788644762333945 1107 64-65 0.1169014684757883 1107 66-67 -0.05741947914895462 1107 68-69 0.09353458213100652 1107 70-71 0.20224911965563308 1107 72-73 0.22675935203266562 1107 74-75 0.27982801160631254 1107 76-77 -0.002661696656296897 1107 78-79 0.09666218399426896 1107 80-81 0.32075815508839867 1107 82-83 0.40306738165304523 1107 84-85 0.23664048159744766 1107 86-87 0.16771449810182304 1107 88-89 0.32375682576778786 1107 90-91 0.21774269852340566 1107 92-93 0.431659178068994 1107 94-95 0.4974217755669734 1107 96-97 0.3114249980161041 1107 98-99 0.32240340115491506 1107 100-101 0.5781406556461839 1108 1 -0.13434464506924115 1108 2 0.010926347771608391 1108 3 -0.06245748276977281 1108 4 -0.12436548287569593 1108 5 -0.05979094379951988 1108 6 -0.06184030252259731 1108 7 0.02926216977262186 1108 8 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-0.6125506701666019 2316 86-87 -0.7504152386911542 2316 88-89 -0.8242948822777585 2316 90-91 -0.8595597095408074 2316 92-93 -0.9423559423342169 2316 94-95 -0.7828393780637946 2316 96-97 -0.832631003477271 2316 98-99 -1.0929813291045605 2316 100-101 -0.9623265834801948 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 11.0 5 22.0 6 71.0 7 117.0 8 266.0 9 518.0 10 934.0 11 1439.0 12 2207.0 13 3486.0 14 4320.0 15 5984.0 16 7992.0 17 11402.0 18 10592.0 19 6451.0 20 5299.0 21 5418.0 22 5545.0 23 6213.0 24 7380.0 25 8836.0 26 10844.0 27 13755.0 28 16966.0 29 21075.0 30 26275.0 31 32743.0 32 41200.0 33 54136.0 34 74341.0 35 110206.0 36 184552.0 37 346100.0 38 427870.0 39 87452.0 40 86.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.08213776672666 18.594268562927205 13.250241887660088 23.073351782686046 2 15.769408382885548 19.83191162583149 40.465349771177735 23.93333022010523 3 20.08808721568139 24.514949666300932 28.660119784087755 26.736843333929922 4 12.499967576849352 15.613886965115285 36.8920983484944 34.994047109540965 5 13.563576610717279 37.99766293930127 33.624622918757964 14.814137531223496 6 32.94795176472724 35.527602476609935 17.206382432358826 14.318063326303996 7 29.89051350489071 32.147164790014706 19.732016175261396 18.230305529833192 8 27.105883634609253 35.34467106065205 19.040300679329853 18.509144625408855 9 27.21929981557712 15.986688351269821 19.838818033497006 36.95519379965606 10-11 25.773895484147925 26.486138067480663 26.687065244105284 21.052901204266128 12-13 26.29155398252584 23.480008339247867 27.664741484937316 22.563696193288983 14-15 22.91493202810698 24.938882361027893 26.12595875256467 26.020226858300454 16-17 22.463707733325723 28.47065216651206 26.453984619011045 22.61165548115117 18-19 22.111453931890633 26.86371512241685 29.170460175292522 21.854370770399996 20-21 23.124360655790856 25.94339240292911 29.144920182283013 21.787326758997022 22-23 22.485008340858208 26.079472410321593 28.96027027947144 22.475248969348765 24-25 22.193271003188922 26.161119154117145 29.42182765558353 22.2237821871104 26-27 22.260544026229883 26.554708058970544 29.12478000178982 22.059967913009757 28-29 21.979646745950067 26.506731566738846 29.372438091050757 22.141183596260333 30-31 22.200635245077756 26.32784767606392 29.3216573437729 22.14985973508542 32-33 21.93554139714346 26.39476030672957 29.264692054536905 22.405006241590065 34-35 22.43651889372293 26.441031001774885 29.051121444964018 22.071328659538167 36-37 22.427561771005514 26.306313375141933 29.011540703725487 22.254584150127066 38-39 22.267196763908082 26.49404353913185 29.09918053675912 22.139579160200945 40-41 22.45636287824405 26.43858055253477 29.0440654357422 22.06099113347898 42-43 22.27362602372573 26.42423440652279 28.86259686039069 22.439542709360786 44-45 22.270567632180597 26.73390926302594 28.694087041841314 22.301436062952142 46-47 22.245775834253482 26.90092728160078 28.258043385558796 22.595253498586946 48-49 22.61646687081331 26.866675465531852 28.045961207381577 22.470896456273262 50-51 22.525440232840996 27.011734462432308 27.8853097267082 22.5775155780185 52-53 22.611224044102126 27.205065739106526 27.756147750291593 22.427562466499758 54-55 22.47286495964599 26.905580824095942 27.930869683068956 22.690684533189113 56-57 22.483202544147428 27.333696918839806 27.715234729895094 22.467865807117672 58-59 22.624830221690186 26.94070915050375 27.89305644405349 22.541404183752576 60-61 22.694884380262774 26.978965436964724 28.0565352922447 22.2696148905278 62-63 22.593715874633943 26.93812625315477 28.07021298119198 22.397944891019307 64-65 22.45764855106297 26.8741051140785 27.953253234558677 22.71499310029985 66-67 22.45776560396548 27.23463596583378 27.701107834211353 22.60649059598939 68-69 22.39570769362457 27.098523579412078 27.85096763650795 22.654801090455404 70-71 22.35104689727577 27.03140416229899 27.988116239852744 22.629432700572497 72-73 22.515461189932864 27.019260262229043 27.817366983834248 22.64791156400385 74-75 22.432086468652003 27.18872478451574 27.650106218241522 22.729082528590734 76-77 22.519266242216997 27.245724911652395 27.58131260594728 22.65369624018333 78-79 22.507558704722832 27.111235894880853 27.652833520238502 22.72837188015781 80-81 22.25654793170308 27.47858425902509 27.62267442237483 22.642193386897002 82-83 22.552246664954726 27.475604821452194 27.387381428538077 22.584767085055002 84-85 22.516919391618462 27.207108008830865 27.365918299919294 22.910054299631376 86-87 22.414425025053816 27.10759652496178 27.647044883259113 22.830933566725285 88-89 22.574388148124953 27.17768125613385 27.502112702011335 22.745817893729868 90-91 22.43743112772936 27.38527359611154 27.59808647299817 22.579208803160935 92-93 22.45742083753789 27.279314037522152 27.535375997101823 22.727889127838136 94-95 22.254058907125902 27.331385088175224 27.554007709759244 22.86054829493963 96-97 22.343845600887906 27.411302641182502 27.399694758068044 22.845156999861548 98-99 22.254949357784003 27.119533268678868 27.380193879096147 23.24532349444098 100-101 22.19783262590895 27.312006738877887 27.379220431406203 23.11094020380696 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 197.0 1 155.0 2 242.5 3 385.5 4 307.5 5 267.0 6 559.5 7 1214.5 8 1893.5 9 2041.5 10 1683.5 11 1438.0 12 1482.0 13 1612.5 14 1715.0 15 1768.0 16 1876.0 17 2019.5 18 2144.5 19 2269.5 20 2504.0 21 2865.5 22 3347.5 23 3963.5 24 4814.0 25 5889.5 26 7463.5 27 9364.5 28 11110.5 29 13132.5 30 15647.5 31 18596.0 32 21880.0 33 25227.0 34 29191.5 35 32786.0 36 36593.0 37 40299.0 38 42397.0 39 44138.5 40 45299.5 41 47742.0 42 50765.0 43 53170.5 44 55577.5 45 56888.0 46 57435.0 47 58404.5 48 62515.0 49 63224.5 50 59597.5 51 56857.0 52 54200.5 53 52895.0 54 51150.5 55 47464.0 56 42723.0 57 38007.5 58 34608.5 59 30834.5 60 26563.5 61 22294.5 62 18056.0 63 14110.5 64 10772.5 65 8441.0 66 6194.0 67 4947.5 68 3653.0 69 2295.5 70 1822.5 71 1533.0 72 1340.5 73 1337.0 74 1096.0 75 786.0 76 517.5 77 270.5 78 155.0 79 82.0 80 41.0 81 26.0 82 15.0 83 7.0 84 2.5 85 2.0 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2726138506511866 2 0.019453890388999993 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.539241090766665E-4 12-13 1.621157532416666E-4 14-15 0.0 16-17 6.160398623183331E-4 18-19 0.0 20-21 3.242315064833332E-5 22-23 3.242315064833332E-5 24-25 0.003274738215481665 26-27 0.003242315064833332 28-29 0.0029180835583499987 30-31 0.001686003833713333 32-33 0.002139927942789999 34-35 0.002269620545383332 36-37 6.484630129666663E-5 38-39 6.484630129666663E-5 40-41 9.726945194499996E-4 42-43 0.0044743947894699985 44-45 0.005155280953084998 46-47 0.015271303955364995 48-49 0.015725228064441658 50-51 0.00723036259457833 52-53 0.00917575163347833 54-55 3.8907780777999983E-4 56-57 0.0038259317765033315 58-59 0.0015238880804716662 60-61 0.0017508501350099995 62-63 0.0 64-65 7.781556155599997E-4 66-67 0.0 68-69 0.0 70-71 2.2696205453833325E-4 72-73 0.0013617723272299994 74-75 0.0 76-77 0.19564129101204325 78-79 0.10800151480959828 80-81 0.0011672334233399995 82-83 0.0 84-85 0.0010699639713949995 86-87 0.12473186054413829 88-89 0.5913009983736548 90-91 0.31398579087845985 92-93 0.2976120998010515 94-95 0.0023668899973283324 96-97 0.0034044308180749983 98-99 0.004182586433634998 100-101 7.457324649116664E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1542108.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.32153580208907 #Duplication Level Percentage of deduplicated Percentage of total 1 75.86991144087558 42.731099335186016 2 13.787936731038291 15.531155444682243 3 4.57674225992921 7.733074591486113 4 2.013333811120399 4.53576209298296 5 1.0420440200426746 2.9344759791093167 6 0.6192974420336959 2.0927869832185793 7 0.3818014157505038 1.5052549474536208 8 0.27146741344311676 1.2231569316269615 9 0.20684890480101487 1.0485043197635946 >10 1.0919577400235572 11.334527336446254 >50 0.09400457944208382 3.643259109158409 >100 0.04257739323354154 4.064681188040769 >500 0.0014999459701552844 0.5432666300859605 >1k 4.615218369708567E-4 0.6799280802140437 >5k 1.1538045924271418E-4 0.3990670305452014 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6141 0.39822113626282984 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4694 0.3043885382865532 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3370 0.2185320353697666 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 6.484630129666663E-5 5 0.0 0.0 0.0 0.0 6.484630129666663E-5 6 0.0 0.0 0.0 0.0 6.484630129666663E-5 7 1.2969260259333327E-4 0.0 0.0 0.0 6.484630129666663E-5 8 1.2969260259333327E-4 0.0 0.0 0.0 1.2969260259333327E-4 9 1.2969260259333327E-4 0.0 0.0 6.484630129666663E-5 1.2969260259333327E-4 10-11 1.9453890388999992E-4 0.0 0.0 6.484630129666663E-5 1.2969260259333327E-4 12-13 2.2696205453833323E-4 0.0 0.0 6.484630129666663E-5 1.2969260259333327E-4 14-15 2.5938520518666654E-4 0.0 0.0 9.726945194499996E-5 1.2969260259333327E-4 16-17 3.5665465713166655E-4 0.0 0.0 1.2969260259333327E-4 1.2969260259333327E-4 18-19 4.5392410907666645E-4 0.0 0.0 1.6211575324166658E-4 1.2969260259333327E-4 20-21 5.187704103733331E-4 0.0 0.0 3.8907780777999983E-4 1.6211575324166658E-4 22-23 5.187704103733331E-4 0.0 0.0 0.0010375408207466664 1.9453890388999992E-4 24-25 5.187704103733331E-4 0.0 0.0 0.001815696436306666 1.9453890388999992E-4 26-27 5.187704103733331E-4 0.0 0.0 0.002010235340196666 1.9453890388999992E-4 28-29 8.105787662083331E-4 6.484630129666663E-5 0.0 0.0027883909557566656 2.5938520518666654E-4 30-31 9.07848218153333E-4 6.484630129666663E-5 0.0 0.004215009584283331 2.918083558349999E-4 32-33 9.07848218153333E-4 6.484630129666663E-5 0.0 0.007327632046523331 3.242315064833332E-4 34-35 0.001005117670098333 6.484630129666663E-5 0.0 0.010861755467191661 3.242315064833332E-4 36-37 0.0010375408207466661 6.484630129666663E-5 0.0 0.018319080116308326 3.242315064833332E-4 38-39 0.001102387122043333 6.484630129666663E-5 0.0 0.03076956996526832 3.242315064833332E-4 40-41 0.001134810272691666 6.484630129666663E-5 0.0 0.04555452666090831 3.8907780777999983E-4 42-43 0.0012969260259333328 6.484630129666663E-5 0.0 0.05969102034358164 3.8907780777999983E-4 44-45 0.0014914649298233328 6.484630129666663E-5 0.0 0.07577290306515497 3.8907780777999983E-4 46-47 0.0015887343817683327 6.484630129666663E-5 0.0 0.09509710085156163 4.2150095842833317E-4 48-49 0.0016211575324166661 6.484630129666663E-5 0.0 0.1189929628793833 4.539241090766665E-4 50-51 0.0016211575324166661 6.484630129666663E-5 0.0 0.14525571490453326 4.539241090766665E-4 52-53 0.0016211575324166661 6.484630129666663E-5 0.0 0.1725235845997816 5.187704103733332E-4 54-55 0.0016211575324166661 6.484630129666663E-5 0.0 0.2018016896352266 6.484630129666664E-4 56-57 0.0016860038337133327 6.484630129666663E-5 0.0 0.24054735465998492 8.430019168566663E-4 58-59 0.0016860038337133327 6.484630129666663E-5 0.0 0.28227594954438984 8.430019168566663E-4 60-61 0.0016860038337133327 6.484630129666663E-5 0.0 0.3317536774337465 9.07848218153333E-4 62-63 0.0016860038337133327 6.484630129666663E-5 0.0 0.3801290182010598 0.0010375408207466661 64-65 0.0016860038337133327 6.484630129666663E-5 0.0 0.4281477043112415 0.0010375408207466661 66-67 0.0016860038337133327 6.484630129666663E-5 0.0 0.48556910410943976 0.0011023871220433327 68-69 0.0016860038337133327 6.484630129666663E-5 0.0 0.5576457680006848 0.001232079724636666 70-71 0.0017184269843616659 6.484630129666663E-5 0.0 0.6364664472267831 0.0012969260259333328 72-73 0.0017508501350099992 6.484630129666663E-5 0.0 0.718659134120308 0.0014266186285266662 74-75 0.0017508501350099992 6.484630129666663E-5 0.0 0.797836468003538 0.0014266186285266662 76-77 0.0017508501350099992 6.484630129666663E-5 0.0 0.8887834055721129 0.0014266186285266662 78-79 0.0017508501350099992 6.484630129666663E-5 0.0 0.9943207609324378 0.0014266186285266662 80-81 0.001815696436306666 6.484630129666663E-5 0.0 1.1245969802374411 0.0014266186285266662 82-83 0.001815696436306666 6.484630129666663E-5 0.0 1.2512418066698312 0.0014266186285266662 84-85 0.0018805427376033326 6.484630129666663E-5 0.0 1.373217699408861 0.0014266186285266662 86-87 0.0019453890388999992 6.484630129666663E-5 0.0 1.5093625089812126 0.0014266186285266662 88-89 0.0019453890388999992 6.484630129666663E-5 0.0 1.6656420951061794 0.0014266186285266662 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7025 0.0 38.125423 1 GGTATCA 4110 0.0 36.228897 1 ATCAACG 8275 0.0 31.867489 3 TCAACGC 8390 0.0 31.43069 4 CAACGCA 8525 0.0 30.877323 5 AACGCAG 8735 0.0 30.29789 6 TATCAAC 8955 0.0 29.451445 2 GTACATG 7880 0.0 27.589422 1 GTACCTG 1425 0.0 27.374842 1 TACATGG 7960 0.0 26.994951 2 ACGCAGA 10120 0.0 26.01079 7 ACATGGG 7990 0.0 25.940346 3 CGCAGAG 10240 0.0 25.705978 8 TACCTGG 1545 0.0 25.175772 2 GCAGAGT 11130 0.0 23.479969 9 GTGTAGC 1115 0.0 22.186108 1 CATGGGG 3295 0.0 22.023031 4 GAGTACT 5735 0.0 21.957672 12-13 AGTACAT 5630 0.0 21.653204 2 AGTACTT 5925 0.0 21.293568 12-13 >>END_MODULE