FastQCFastQC Report
Thu 26 May 2016
SRR936303_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936303_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847593
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43820.23717344674936527No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30060.1626981699973966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21410.11588049965549772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36300.034.8258931
GTATCAA56250.034.2585951
TCAACGC64950.029.0786234
ATCAACG65400.028.8061643
CAACGCA66550.028.4506385
TATCAAC66900.028.0895332
AACGCAG69400.027.4172046
GTACATG69000.027.3106251
TACATGG68650.026.2013192
ACATGGG69500.025.336013
GTACCTG17650.024.6797521
TACCTGG19000.023.1691152
ACGCAGA81900.022.82817
CGCAGAG85650.021.7186838
GTGTAGC10700.021.682531
CATGGGG43100.020.3177014
GAGTACA52000.020.3048231
GCAGAGT91650.019.8320269
TAACGCA2201.3913377E-619.3642164
AGTACAT49800.019.2950882
Produced by FastQC (version 0.11.5)