##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936303_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1847593 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.508041002536814 33.0 31.0 34.0 30.0 34.0 2 31.607069847092948 33.0 31.0 34.0 30.0 34.0 3 31.673262996774724 34.0 31.0 34.0 30.0 34.0 4 35.160098571492746 37.0 35.0 37.0 33.0 37.0 5 35.13661720952612 37.0 35.0 37.0 33.0 37.0 6 35.2234133816268 37.0 35.0 37.0 33.0 37.0 7 35.16200808294901 37.0 35.0 37.0 32.0 37.0 8 35.16491781469187 37.0 35.0 37.0 32.0 37.0 9 36.80095832794344 39.0 37.0 39.0 33.0 39.0 10-11 36.70920570710108 39.0 37.0 39.0 33.0 39.0 12-13 36.6018335748187 39.0 37.0 39.0 32.0 39.0 14-15 37.80261183063586 40.0 38.0 41.0 32.5 41.0 16-17 37.74391654439046 40.0 38.0 41.0 32.0 41.0 18-19 37.681721299009034 40.0 38.0 41.0 32.0 41.0 20-21 37.59720836786023 40.0 38.0 41.0 32.0 41.0 22-23 37.421659423909915 40.0 37.5 41.0 32.0 41.0 24-25 37.58948805283414 40.0 38.0 41.0 32.0 41.0 26-27 37.53904783142175 40.0 38.0 41.0 32.0 41.0 28-29 37.40064072552775 40.0 38.0 41.0 32.0 41.0 30-31 37.23978468201601 40.0 37.0 41.0 31.0 41.0 32-33 37.05311045235612 40.0 37.0 41.0 31.0 41.0 34-35 36.79088278641454 40.0 36.5 41.0 30.0 41.0 36-37 36.602323401311864 40.0 36.0 41.0 30.0 41.0 38-39 36.43365070120963 40.0 36.0 41.0 30.0 41.0 40-41 36.244511913608676 40.0 36.0 41.0 29.5 41.0 42-43 36.0546622010367 40.0 35.5 41.0 29.0 41.0 44-45 35.86880065035968 39.0 35.0 41.0 28.0 41.0 46-47 35.59930812684395 39.0 35.0 41.0 27.0 41.0 48-49 35.42500377518209 39.0 35.0 41.0 26.0 41.0 50-51 35.124621872890835 38.5 34.5 40.5 26.0 41.0 52-53 35.15661566156616 39.0 35.0 40.0 26.0 41.0 54-55 35.236607845992054 39.0 35.0 41.0 26.0 41.0 56-57 35.08058132932957 39.0 35.0 41.0 26.0 41.0 58-59 34.84876214620861 38.5 35.0 41.0 26.0 41.0 60-61 34.54538418363785 38.0 34.0 40.0 25.0 41.0 62-63 34.229937545769005 37.5 34.0 40.0 23.5 41.0 64-65 33.892950449584944 37.0 34.0 40.0 23.0 41.0 66-67 33.51373219101826 36.5 33.5 39.5 22.0 41.0 68-69 33.12003076435124 36.0 33.0 39.0 21.5 41.0 70-71 32.705578014205514 35.5 33.0 39.0 20.0 40.5 72-73 32.28351942229701 35.0 32.5 38.0 18.5 40.0 74-75 31.847938912953232 35.0 32.0 37.0 17.5 39.5 76-77 31.43187785405119 35.0 32.0 37.0 14.0 39.0 78-79 31.027499021700127 35.0 31.0 36.0 9.5 39.0 80-81 30.60815206595825 35.0 31.0 36.0 7.0 37.5 82-83 30.245959743298442 35.0 31.0 35.0 3.5 37.0 84-85 29.905772537566445 34.0 30.5 35.0 2.0 37.0 86-87 29.566960364106166 34.0 30.0 35.0 2.0 36.0 88-89 29.22035020699905 34.0 30.0 35.0 2.0 36.0 90-91 28.844685490798028 34.0 29.0 35.0 2.0 35.5 92-93 28.50666380528612 34.0 29.0 35.0 2.0 35.0 94-95 28.095941313914913 34.0 29.0 35.0 2.0 35.0 96-97 27.635599128162966 34.0 28.0 35.0 2.0 35.0 98-99 27.084347039634814 34.0 27.0 35.0 2.0 35.0 100-101 23.76367901372218 29.5 13.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4102643745661574 1101 2 0.39359151969278017 1101 3 0.3088743902510629 1101 4 0.15300627772231223 1101 5 0.4485038035641935 1101 6 0.30159177503455226 1101 7 0.4059821563829402 1101 8 0.3361888168899583 1101 9 0.3330264661799447 1101 10-11 0.569616164173091 1101 12-13 0.5187475478575791 1101 14-15 0.7095490609804003 1101 16-17 0.7297452361706434 1101 18-19 0.7712554324234588 1101 20-21 0.8119913936236713 1101 22-23 0.7594617190929611 1101 24-25 0.676715921131148 1101 26-27 0.6426068108348986 1101 28-29 0.7562308306223429 1101 30-31 0.6722917961656023 1101 32-33 0.720706923961508 1101 34-35 0.5608968118170807 1101 36-37 0.5864548317096379 1101 38-39 0.6642239499217979 1101 40-41 0.5578598701274728 1101 42-43 0.5344940372253575 1101 44-45 0.4937137403200751 1101 46-47 0.4991225547930469 1101 48-49 0.48963388887293036 1101 50-51 0.5512402347973335 1101 52-53 0.7569953008870485 1101 54-55 0.6735966342362616 1101 56-57 0.5681174769571342 1101 58-59 0.5994766612334885 1101 60-61 0.4952258282571478 1101 62-63 0.4555143812779434 1101 64-65 0.5993875331620089 1101 66-67 0.6398872898962011 1101 68-69 0.6246081706858746 1101 70-71 0.8151020961147353 1101 72-73 0.9038008565634215 1101 74-75 0.5311041774691354 1101 76-77 0.6056355640310152 1101 78-79 0.7208818559569501 1101 80-81 0.8260128841076906 1101 82-83 0.9812227714688149 1101 84-85 0.9075145152601145 1101 86-87 0.8283325815057658 1101 88-89 0.6501859901873139 1101 90-91 1.0161695185587405 1101 92-93 1.080732159555449 1101 94-95 1.1617706318272703 1101 96-97 1.2665838464987331 1101 98-99 1.3908231709622392 1101 100-101 1.367352851239989 1102 1 0.3474381348362101 1102 2 0.3490335664633619 1102 3 0.35111119381129896 1102 4 0.2428496067601742 1102 5 0.4428806031476995 1102 6 0.4811448636017559 1102 7 0.36769893438282963 1102 8 0.4112419790721802 1102 9 0.49380772637790216 1102 10-11 0.4871060667735989 1102 12-13 0.5404055400953709 1102 14-15 0.591407210162366 1102 16-17 0.6696048060464648 1102 18-19 0.6853015564032816 1102 20-21 0.551296915660366 1102 22-23 0.6755656483132597 1102 24-25 0.7632192535386011 1102 26-27 0.7331604868043797 1102 28-29 0.7375047270422286 1102 30-31 0.7054170342760173 1102 32-33 0.64394505866332 1102 34-35 0.5118314568637032 1102 36-37 0.44110066001513815 1102 38-39 0.5539653563176543 1102 40-41 0.6489049452830855 1102 42-43 0.6688846946453637 1102 44-45 0.6579738790805791 1102 46-47 0.6961979137990397 1102 48-49 0.551989599652444 1102 50-51 0.4344911876184625 1102 52-53 0.44628473386930523 1102 54-55 0.4123972862983152 1102 56-57 0.533069900408897 1102 58-59 0.4689329574420853 1102 60-61 0.555934687710149 1102 62-63 0.7192638822895816 1102 64-65 0.4964065804774904 1102 66-67 0.5466322465088709 1102 68-69 0.5409896740410431 1102 70-71 0.609939900232014 1102 72-73 0.568525642662479 1102 74-75 0.6886203841558078 1102 76-77 0.6420376188764756 1102 78-79 0.622587789023445 1102 80-81 0.5677864063811633 1102 82-83 0.6913213472839317 1102 84-85 0.6687451685516201 1102 86-87 0.5235087490626462 1102 88-89 0.7024166009794257 1102 90-91 0.7205452860142252 1102 92-93 0.5734901877659375 1102 94-95 0.5755968594145351 1102 96-97 0.8228420333230098 1102 98-99 0.8575531693077991 1102 100-101 0.714208579474608 1103 1 0.38519170497058397 1103 2 0.3885557489968825 1103 3 0.47204981930031664 1103 4 0.3696346739474805 1103 5 0.37746413061055506 1103 6 0.3937673500866481 1103 7 0.42084790013468165 1103 8 0.3136114685669966 1103 9 0.34102742183490165 1103 10-11 0.4675084140768533 1103 12-13 0.6290513664955029 1103 14-15 0.5792879813557548 1103 16-17 0.5845294837914565 1103 18-19 0.30328978290991415 1103 20-21 0.6253440170292563 1103 22-23 0.7013127696497534 1103 24-25 0.6565940751243247 1103 26-27 0.6144216261413007 1103 28-29 0.699999827582829 1103 30-31 0.7333820391939696 1103 32-33 0.7386214724809079 1103 34-35 0.6255959796008952 1103 36-37 0.5945367525760901 1103 38-39 0.7508925632930001 1103 40-41 0.7875356110918901 1103 42-43 0.608530750303224 1103 44-45 0.7269161135298816 1103 46-47 0.7795608287740095 1103 48-49 0.7777877493207583 1103 50-51 0.6724013555682262 1103 52-53 0.7577226604920426 1103 54-55 0.8095252786662499 1103 56-57 0.8654788074411996 1103 58-59 0.8063892320780468 1103 60-61 0.7151061059280082 1103 62-63 0.7475134256229836 1103 64-65 0.8085857121084032 1103 66-67 0.8344002376935507 1103 68-69 0.7867522887623437 1103 70-71 0.7993775902016225 1103 72-73 0.6935023471196899 1103 74-75 0.8086144308504295 1103 76-77 0.738906956765387 1103 78-79 0.8498090005395795 1103 80-81 0.7849568853818951 1103 82-83 0.9399504230794911 1103 84-85 1.0221479817657375 1103 86-87 0.9756826299521641 1103 88-89 0.9492210441419999 1103 90-91 1.169698141751951 1103 92-93 1.404699189459489 1103 94-95 1.3119133164213181 1103 96-97 1.2389096717466046 1103 98-99 1.2751477750954443 1103 100-101 1.6314746441548706 1104 1 0.3910562211934412 1104 2 0.27749751831379044 1104 3 0.282590314639247 1104 4 0.33299326384582173 1104 5 0.32141964318309135 1104 6 0.22898680588281195 1104 7 0.3043265435517313 1104 8 0.32579860953353545 1104 9 0.3191301981179677 1104 10-11 0.32013962944603236 1104 12-13 0.34664164296035693 1104 14-15 0.5060533352627701 1104 16-17 0.4563456295717714 1104 18-19 0.3530872698574541 1104 20-21 0.41440067705455874 1104 22-23 0.5385515387194388 1104 24-25 0.48202564243719337 1104 26-27 0.37992369126543224 1104 28-29 0.43428961394428 1104 30-31 0.4739984496592058 1104 32-33 0.543942439308033 1104 34-35 0.5151172925544572 1104 36-37 0.5895853629802517 1104 38-39 0.5587304001481996 1104 40-41 0.5175224495613762 1104 42-43 0.674026634598107 1104 44-45 0.6131050505726847 1104 46-47 0.6350112294371826 1104 48-49 0.6556156604913355 1104 50-51 0.37656196259837316 1104 52-53 0.35946110961291566 1104 54-55 0.5399184157643191 1104 56-57 0.5676119206885275 1104 58-59 0.6060358340762733 1104 60-61 0.4987789000991185 1104 62-63 0.5674540533043739 1104 64-65 0.6258747640419031 1104 66-67 0.5487815730435273 1104 68-69 0.4490794073324693 1104 70-71 0.30132746004063193 1104 72-73 0.5101685896118084 1104 74-75 0.3916262398700745 1104 76-77 0.5611773934955018 1104 78-79 0.46039221631596305 1104 80-81 0.5943248410544442 1104 82-83 0.7223352415085422 1104 84-85 0.5444829536247866 1104 86-87 0.5122222381603017 1104 88-89 0.45469689527854484 1104 90-91 0.7268135487413829 1104 92-93 0.7200132846588012 1104 94-95 0.508453324565469 1104 96-97 0.7361646564036057 1104 98-99 0.9672466756349962 1104 100-101 1.1907486148650115 1105 1 0.2847116787905719 1105 2 0.3132871459815547 1105 3 0.1939213344863262 1105 4 0.11031204619986568 1105 5 0.13237368329160404 1105 6 0.2126599995857248 1105 7 0.22840880156658017 1105 8 0.3087596292966239 1105 9 0.16669592809800804 1105 10-11 0.23931261177151697 1105 12-13 0.3355810742291041 1105 14-15 0.3319873505099409 1105 16-17 0.3663989358786637 1105 18-19 0.34088442312148715 1105 20-21 0.30143519176839106 1105 22-23 0.4663853960634654 1105 24-25 0.33788443671795676 1105 26-27 0.3337148013748745 1105 28-29 0.3816174393325298 1105 30-31 0.6519694923518387 1105 32-33 0.5197645715043109 1105 34-35 0.32825719921242325 1105 36-37 0.3935021796642246 1105 38-39 0.36697975411718886 1105 40-41 0.38593475312735137 1105 42-43 0.4167808334496641 1105 44-45 0.4357084715273203 1105 46-47 0.5101708123937172 1105 48-49 0.6040476179582726 1105 50-51 0.5105852239140631 1105 52-53 0.5039209563012861 1105 54-55 0.5867159427884303 1105 56-57 0.6488231973241838 1105 58-59 0.68631865987048 1105 60-61 0.6512558655592073 1105 62-63 0.5121734322747074 1105 64-65 0.4924590378414635 1105 66-67 0.4422393017581001 1105 68-69 0.42581564190261645 1105 70-71 0.369814095667941 1105 72-73 0.4466029534217455 1105 74-75 0.43722392003783384 1105 76-77 0.34288838176195924 1105 78-79 0.44682888987922453 1105 80-81 0.49871295240415137 1105 82-83 0.49656647861241865 1105 84-85 0.4520077799733322 1105 86-87 0.4888055390637689 1105 88-89 0.4686922505745983 1105 90-91 0.5594388493592746 1105 92-93 0.4550130116879565 1105 94-95 0.3591762423578473 1105 96-97 0.45334656137615426 1105 98-99 0.5835936747754893 1105 100-101 0.6438013038378294 1106 1 0.317177284952205 1106 2 0.4508145317776844 1106 3 0.39863849534471285 1106 4 0.2757967577815066 1106 5 0.3095274655932343 1106 6 0.2678095498463122 1106 7 0.18728247990424052 1106 8 0.25672661665493024 1106 9 0.3597416368628217 1106 10-11 0.35042086901430025 1106 12-13 0.48459229390061154 1106 14-15 0.3474269860917403 1106 16-17 0.3695369058597464 1106 18-19 0.40732950864681783 1106 20-21 0.24860424888053245 1106 22-23 0.38248722359759313 1106 24-25 0.3508472736823194 1106 26-27 0.3969788820966329 1106 28-29 0.46142186052266965 1106 30-31 0.3770361708119765 1106 32-33 0.4532155560321911 1106 34-35 0.4832504293075459 1106 36-37 0.5748682716260731 1106 38-39 0.47770920286323104 1106 40-41 0.4722222937807956 1106 42-43 0.6450764843811214 1106 44-45 0.5563216412035956 1106 46-47 0.609007001107372 1106 48-49 0.5422713854273553 1106 50-51 0.49758955801397775 1106 52-53 0.47388333059878107 1106 54-55 0.565851708297977 1106 56-57 0.5747917359772785 1106 58-59 0.5746210126675706 1106 60-61 0.6554176560799831 1106 62-63 0.5604022006282889 1106 64-65 0.5409618342544391 1106 66-67 0.5793382104086078 1106 68-69 0.6210722859349005 1106 70-71 0.7241840862785267 1106 72-73 0.7674137771048493 1106 74-75 0.6110946386683445 1106 76-77 0.6098310042666206 1106 78-79 0.6933411981786541 1106 80-81 0.7629336649111238 1106 82-83 0.6178370774938564 1106 84-85 0.6765473243825468 1106 86-87 0.6338451379043768 1106 88-89 0.5663651382962378 1106 90-91 0.689783672253121 1106 92-93 0.6753793690028544 1106 94-95 0.7862267751051384 1106 96-97 0.7840491006007788 1106 98-99 0.6157959690588335 1106 100-101 0.7084547459432358 1107 1 0.19097726204007515 1107 2 0.28482186365991424 1107 3 0.3647314040398655 1107 4 0.27657577026861446 1107 5 0.16837441255164975 1107 6 0.2512927667096392 1107 7 0.14319094593700754 1107 8 0.33737732120299313 1107 9 0.27919690092514315 1107 10-11 0.22433683877028443 1107 12-13 0.30275302250640124 1107 14-15 0.3507807494094166 1107 16-17 0.30690659310914015 1107 18-19 0.3276582977406406 1107 20-21 0.4425225098497094 1107 22-23 0.4612304931588227 1107 24-25 0.41098302806426545 1107 26-27 0.3744834645230313 1107 28-29 0.48160028875042116 1107 30-31 0.36887229178803693 1107 32-33 0.41250785755585184 1107 34-35 0.4706659408208935 1107 36-37 0.44753696219773076 1107 38-39 0.4321026041692235 1107 40-41 0.45219995445211936 1107 42-43 0.08153541438456102 1107 44-45 0.31025250384536207 1107 46-47 0.34574230870273936 1107 48-49 0.24610343949998992 1107 50-51 0.40971007582815844 1107 52-53 0.31801639280521954 1107 54-55 0.36651616012522226 1107 56-57 0.29122689464379903 1107 58-59 0.35451704886873614 1107 60-61 0.5259225821883504 1107 62-63 0.6543562618036844 1107 64-65 0.3275682611074586 1107 66-67 0.49117232657311405 1107 68-69 0.4919810383693104 1107 70-71 0.6524275849610817 1107 72-73 0.3584064452226201 1107 74-75 0.39071143326107105 1107 76-77 0.39049746090540793 1107 78-79 0.4381186640968586 1107 80-81 0.5358228847530313 1107 82-83 0.5118899472383838 1107 84-85 0.5296118220340524 1107 86-87 0.2938162113377629 1107 88-89 0.5368586083386226 1107 90-91 0.6533476543816548 1107 92-93 0.4353891066589419 1107 94-95 0.7856883400037518 1107 96-97 0.6518904913671904 1107 98-99 0.6883028426984232 1107 100-101 0.5648529587969406 1108 1 -0.06757984709174991 1108 2 -0.0365272321144694 1108 3 -0.014614186512623917 1108 4 -0.10592811367205002 1108 5 -0.03085308408964238 1108 6 -0.1488042626977304 1108 7 0.03792716969621779 1108 8 -0.11743489510347871 1108 9 -0.025640609372139522 1108 10-11 -0.09194171026688736 1108 12-13 -0.11588567702310115 1108 14-15 -0.2902909555990689 1108 16-17 -0.19456405203966654 1108 18-19 -0.09808379754168328 1108 20-21 -0.16899911029259584 1108 22-23 -0.1045387205988959 1108 24-25 -0.27521046129014337 1108 26-27 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68-69 -2.176963500589167 2316 70-71 -2.0565323102049824 2316 72-73 -2.0681726995949354 2316 74-75 -1.934112981384743 2316 76-77 -2.294494680729091 2316 78-79 -2.4846032720288456 2316 80-81 -2.2476581151403785 2316 82-83 -2.345881213396222 2316 84-85 -2.515447204531821 2316 86-87 -2.466749084155591 2316 88-89 -2.4039774088912615 2316 90-91 -2.5973812299185397 2316 92-93 -2.6708585812887087 2316 94-95 -2.5784007322702216 2316 96-97 -2.7980777407722677 2316 98-99 -2.7154424992345234 2316 100-101 -2.463725967616572 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21820.0 3 4501.0 4 3733.0 5 3028.0 6 2861.0 7 3133.0 8 3524.0 9 4323.0 10 4892.0 11 5722.0 12 7085.0 13 9814.0 14 9874.0 15 9056.0 16 10131.0 17 12505.0 18 11868.0 19 9511.0 20 9251.0 21 9811.0 22 10457.0 23 11357.0 24 12919.0 25 15069.0 26 17238.0 27 20579.0 28 24941.0 29 29582.0 30 35960.0 31 44173.0 32 55799.0 33 73294.0 34 101165.0 35 147131.0 36 238330.0 37 395582.0 38 386003.0 39 71522.0 40 49.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.172756055680914 17.60099028719303 12.951654729395687 24.27459892773037 2 15.420200295663724 18.776920041421068 41.203246346560974 24.59963331635423 3 20.32584048885817 23.011226004881436 28.046716289372075 28.616217216888316 4 12.822254614501505 14.795741900772452 36.14918827750717 36.23281520721887 5 14.21468940009451 37.114798253367766 33.620667723624194 15.049844622913533 6 34.429301450230824 34.52683871547427 16.745611229018255 14.298248605276648 7 30.593808491267648 31.277598135424235 19.458424312232005 18.670169061076113 8 26.93697575424268 36.1187193355476 18.430878589337755 18.513426320871964 9 26.85456398752572 16.49144896853335 19.22735680480003 37.4266302391409 10-11 26.105639303055778 25.927268042939005 26.48741496175207 21.479677692253148 12-13 26.506912008366186 22.80889686630721 27.392815018861267 23.291376106465336 14-15 23.167662510348844 24.482679573255474 25.21139519066638 27.138262725729305 16-17 23.228805005536653 27.758765874067425 25.735849996305287 23.276579124090635 18-19 23.15606653871939 26.702259502737803 27.579937051238364 22.561736907304443 20-21 23.853885603713078 25.870827187724117 27.72189821517977 22.553388993383038 22-23 23.353044643695668 25.8098809878552 27.691752238869793 23.145322129579334 24-25 23.056815579581276 25.93622085906841 27.832374307528152 23.174589253822163 26-27 23.12924636149879 26.142322665015392 27.682731571494763 23.045699401991055 28-29 23.078959341970748 26.30893584517939 27.5967256608185 23.01537915203136 30-31 23.30714509396126 26.11636087857587 27.57492500665149 23.00156902081138 32-33 23.017584809470154 26.19820213856357 27.692495486129815 23.091717565836465 34-35 23.287189542802864 26.272684921776413 27.61068391408524 22.829441621335487 36-37 23.268516572735965 26.18748642420212 27.614756440331327 22.929240562730584 38-39 23.192555651156248 26.411290487006234 27.56219595242076 22.833957909416757 40-41 23.435120813719777 26.313120491781884 27.48677988149205 22.76497881300629 42-43 23.118553933196758 26.210844413510724 27.45974500525405 23.210856648038465 44-45 23.256089668637188 26.388129021030366 27.333190121657413 23.022591188675037 46-47 23.18133549793262 26.40841039279591 27.1827985059914 23.227455603280074 48-49 23.3784133281372 26.471672051808316 27.104780869017997 23.045133751036484 50-51 23.159112655500426 26.625747481830064 26.999753581901924 23.215386280767582 52-53 23.295545556774808 26.719126271547122 26.902639705312453 23.082688466365617 54-55 23.229546644188087 26.42132383432017 27.110636764715878 23.238492756775862 56-57 23.210331461187415 26.618641265808467 27.02714901614454 23.14387825685958 58-59 23.33070406052711 26.720100450728594 26.970221242294695 22.9789742464496 60-61 23.402575368686648 26.593482185583156 27.09281332927307 22.911129116457126 62-63 23.357137536017607 26.599741929309513 27.063355647620945 22.97976488705193 64-65 23.163469557738175 26.560019926921928 27.0670034592027 23.209507056137195 66-67 23.23762055395282 26.715620443036116 26.903743873755044 23.14301512925602 68-69 23.026375363741156 26.554729158491753 27.086842937513882 23.332052540253205 70-71 23.145235771968693 26.47197241843567 27.012032541721847 23.37075926787379 72-73 23.22296079440603 26.64455572422445 26.88551387956497 23.246969601804548 74-75 23.009390067141837 26.839873928898726 26.861384334659494 23.289351669299947 76-77 23.163024560256744 26.748538720871963 26.82293160721535 23.265505111655944 78-79 23.17574300835965 26.645113838900315 26.838572673497772 23.340570479242263 80-81 23.22722125015125 26.84006237568708 26.679201623834363 23.25351475032731 82-83 23.242786286909446 26.890354606419315 26.78474463405356 23.08211447261768 84-85 23.40718557012745 26.797398594340383 26.566575232808297 23.228840602723874 86-87 23.128043066960117 26.865349402412754 26.790219331948744 23.216388198678384 88-89 23.208331463676306 26.7716122154552 26.711783190491534 23.308273130376964 90-91 23.236826385420645 26.825846822762127 26.73874629645951 23.19858049535772 92-93 23.24184215357626 26.84028368022738 26.721861464502574 23.196012701693785 94-95 23.16074634669866 26.872875201110695 26.724766368989755 23.24161208320089 96-97 23.083526632308242 26.819223572860963 26.686050478555977 23.411199316274818 98-99 23.13646877828546 26.72224321216849 26.562091432205946 23.5791965773401 100-101 23.22008935707055 26.963479728297095 26.274925762302363 23.541505152329993 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 64.0 1 64.5 2 136.5 3 231.0 4 217.5 5 230.0 6 427.0 7 843.0 8 1374.5 9 1603.5 10 1520.0 11 1455.0 12 1483.5 13 1630.5 14 1816.0 15 1983.0 16 2171.0 17 2317.5 18 2405.5 19 2499.0 20 2631.5 21 2947.0 22 3347.5 23 3944.0 24 4781.5 25 5961.5 26 7490.5 27 9275.5 28 11066.5 29 12880.5 30 15645.0 31 18609.5 32 21490.0 33 24963.0 34 28941.0 35 32467.5 36 36510.0 37 40472.0 38 43412.5 39 46179.0 40 48883.0 41 51822.0 42 54893.0 43 59457.0 44 63660.0 45 66531.0 46 68907.0 47 71346.0 48 74533.5 49 75638.5 50 74023.0 51 72421.5 52 71956.5 53 71088.5 54 69844.0 55 66379.0 56 60849.0 57 55803.5 58 51377.0 59 46172.0 60 39069.0 61 31790.5 62 25152.0 63 19659.5 64 14940.0 65 10944.0 66 8286.0 67 6727.0 68 5300.0 69 3738.0 70 2533.5 71 1757.5 72 1253.0 73 941.5 74 715.0 75 536.0 76 381.0 77 225.5 78 126.5 79 78.0 80 52.0 81 38.5 82 33.0 83 27.0 84 22.0 85 20.0 86 20.0 87 17.5 88 15.5 89 15.0 90 14.0 91 13.0 92 16.0 93 18.0 94 13.0 95 10.5 96 14.0 97 10.5 98 10.5 99 12.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04633055007244561 2 0.012123882261948384 3 0.010067152235367855 4 0.00552069638713721 5 0.008226920106322118 6 0.005087700592067625 7 0.005141825066451324 8 0.009580031965914572 9 0.014721857032365894 10-11 0.002273227924115322 12-13 0.008443418003856909 14-15 0.008903476036118345 16-17 0.019403624066555783 18-19 0.011772073178454346 20-21 0.0036804642580914736 22-23 0.009552969728722722 24-25 0.021974536599781443 26-27 0.00698205719549706 28-29 0.017292769565591556 30-31 0.014207674525720764 32-33 0.01252981581982612 34-35 0.2579843071498972 36-37 0.4536984065213497 38-39 0.45169580096915285 40-41 0.4724525368953011 42-43 0.46197945110205546 44-45 0.47754023748736874 46-47 0.48254675136786074 48-49 0.4896370575121252 50-51 0.5008678859467426 52-53 0.48904168829390454 54-55 0.47659305918565403 56-57 0.4711535495100923 58-59 0.4698816243620754 60-61 0.467256587354466 62-63 0.4634137496732235 64-65 0.48032764791812915 66-67 0.45329247296347197 68-69 0.4810853905595009 70-71 0.4621418245252066 72-73 0.47004399778522654 74-75 0.48430579678533103 76-77 0.4712888606960516 78-79 0.47090998937536566 80-81 0.4728043459787951 82-83 0.4736703375689343 84-85 0.47310203058790545 86-87 0.47532113403763704 88-89 0.4882298211781491 90-91 0.5134788884781443 92-93 0.5009490726583182 94-95 0.47318321729948104 96-97 0.44693284722338744 98-99 0.4377587488153506 100-101 0.4454985486522194 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1847593.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.64878204391186 #Duplication Level Percentage of deduplicated Percentage of total 1 77.49919014965812 48.552298722656126 2 13.049448794977451 16.350641466994613 3 4.284265167255342 8.052119840451102 4 1.871345041838957 4.689499506204957 5 0.9866963896564622 3.090766352955121 6 0.5709156880345426 2.146030350307562 7 0.38220200691828726 1.676114315971863 8 0.24557875465772613 1.230816790013375 9 0.19863139813095662 1.1199613651725404 >10 0.8587031748235198 9.349797602045314 >50 0.03960851831400313 1.696957360954279 >100 0.012809352346503797 1.3711666467171122 >500 3.460362217256189E-4 0.15738770927619786 >1k 2.595271662942131E-4 0.516441970279919 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4382 0.23717344674936527 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3006 0.1626981699973966 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2141 0.11588049965549772 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 5.412447438369814E-5 0.0 0.0 0.0 4 0.0 5.412447438369814E-5 0.0 0.0 0.0 5 0.0 1.0824894876739628E-4 0.0 0.0 0.0 6 0.0 1.0824894876739628E-4 0.0 0.0 0.0 7 0.0 1.0824894876739628E-4 0.0 0.0 0.0 8 0.0 1.0824894876739628E-4 0.0 0.0 0.0 9 0.0 1.0824894876739628E-4 0.0 5.412447438369814E-5 0.0 10-11 0.0 1.0824894876739628E-4 0.0 5.412447438369814E-5 0.0 12-13 0.0 1.0824894876739628E-4 0.0 5.412447438369814E-5 0.0 14-15 0.0 1.3531118595924536E-4 0.0 8.118671157554722E-5 0.0 16-17 0.0 1.6237342315109443E-4 0.0 1.3531118595924536E-4 0.0 18-19 0.0 1.6237342315109443E-4 0.0 2.1649789753479257E-4 0.0 20-21 0.0 1.6237342315109443E-4 0.0 3.2474684630218886E-4 0.0 22-23 0.0 2.1649789753479257E-4 0.0 5.953692182206796E-4 0.0 24-25 0.0 2.1649789753479257E-4 0.0 0.0010013027760984157 0.0 26-27 0.0 2.1649789753479257E-4 0.0 0.0012448629108250573 0.0 28-29 0.0 2.1649789753479257E-4 0.0 0.0017049209430864915 0.0 30-31 0.0 2.1649789753479257E-4 0.0 0.0029227216167196997 0.0 32-33 0.0 2.4356013472664166E-4 0.0 0.004898264931724682 0.0 34-35 0.0 2.706223719184907E-4 0.0 0.007360928516182948 0.0 36-37 0.0 3.5180908349403793E-4 0.0 0.013314620698389745 0.0 38-39 0.0 5.141825066451324E-4 0.0 0.023138212799030958 0.0 40-41 0.0 5.953692182206796E-4 0.0 0.0335842363550847 0.0 42-43 0.0 5.953692182206796E-4 0.0 0.04484212702689391 0.0 44-45 0.0 5.953692182206796E-4 0.0 0.05750725403267927 0.0 46-47 0.0 6.224314554125287E-4 0.0 0.07336572502710283 0.0 48-49 0.0 6.494936926043777E-4 0.0 0.09147036170844985 0.0 50-51 0.0 6.494936926043777E-4 0.0 0.11114460814692413 0.0 52-53 0.0 6.494936926043777E-4 0.0 0.13349801606739148 0.0 54-55 0.0 7.57742641371774E-4 0.0 0.1570151001871083 0.0 56-57 0.0 8.389293529473212E-4 0.0 0.18488920449471286 0.0 58-59 0.0 9.201160645228684E-4 0.0 0.2190417478308264 0.0 60-61 0.0 9.742405389065665E-4 0.0 0.25387084709673613 0.0 62-63 0.0 0.0010283650132902648 0.0 0.28926825334367473 0.0 64-65 0.0 0.0010554272504821138 0.0 0.32915799096446025 0.0 66-67 0.0 0.001136613962057661 0.0 0.37573210117163247 0.0 68-69 0.0 0.001136613962057661 0.0 0.42912589515115074 0.0 70-71 0.0 0.0011636761992495101 0.0 0.4891769994798638 0.0 72-73 0.0 0.0012178006736332082 0.0 0.5522861366112558 0.0 74-75 0.0 0.0012448629108250573 0.0 0.6169107590253914 0.0 76-77 0.0 0.0012989873852087554 0.0 0.6877867582308441 0.0 78-79 0.0 0.0012989873852087554 0.0 0.774061170398459 0.0 80-81 0.0 0.0013260496224006045 0.0 0.8718099711354178 0.0 82-83 0.0 0.0013531118595924536 0.0 0.9708848214947773 0.0 84-85 0.0 0.0013531118595924536 0.0 1.0749932479718205 0.0 86-87 0.0 0.0013531118595924536 0.0 1.187518030215529 0.0 88-89 0.0 0.0013801740967843026 0.0 1.3163884037231144 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3630 0.0 34.825893 1 GTATCAA 5625 0.0 34.258595 1 TCAACGC 6495 0.0 29.078623 4 ATCAACG 6540 0.0 28.806164 3 CAACGCA 6655 0.0 28.450638 5 TATCAAC 6690 0.0 28.089533 2 AACGCAG 6940 0.0 27.417204 6 GTACATG 6900 0.0 27.310625 1 TACATGG 6865 0.0 26.201319 2 ACATGGG 6950 0.0 25.33601 3 GTACCTG 1765 0.0 24.679752 1 TACCTGG 1900 0.0 23.169115 2 ACGCAGA 8190 0.0 22.8281 7 CGCAGAG 8565 0.0 21.718683 8 GTGTAGC 1070 0.0 21.68253 1 CATGGGG 4310 0.0 20.317701 4 GAGTACA 5200 0.0 20.304823 1 GCAGAGT 9165 0.0 19.832026 9 TAACGCA 220 1.3913377E-6 19.364216 4 AGTACAT 4980 0.0 19.295088 2 >>END_MODULE