FastQCFastQC Report
Thu 26 May 2016
SRR936303_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936303_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847593
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45700.2473488479335005No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33000.17861076546620386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22980.12437804213373832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54900.034.054831
GGTATCA36850.032.403711
GTACCTG16350.028.8055341
ATCAACG65750.028.0657393
TCAACGC66100.027.917134
GTACATG62550.027.6081811
CAACGCA67700.027.2573475
TATCAAC68400.026.8469562
TACATGG63850.026.3819962
AACGCAG70600.026.2049036
ACATGGG65350.023.9547163
TACCTGG17850.023.924592
GTGTAGC11450.022.0205841
ACGCAGA84050.021.8421757
CATGGGG39950.021.7298624
CGCAGAG85050.021.4738068
TAACGCA1456.2026456E-419.6293494
AGAGTAC74500.019.38944610-11
GCAGAGT93450.019.2399
GAGTACT44600.019.09255212-13
Produced by FastQC (version 0.11.5)