##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936303_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1847593 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.47144365669279 34.0 31.0 34.0 31.0 34.0 2 32.60195941422164 34.0 31.0 34.0 31.0 34.0 3 32.687314251569475 34.0 31.0 34.0 31.0 34.0 4 36.17391113735547 37.0 35.0 37.0 35.0 37.0 5 36.13493826833074 37.0 35.0 37.0 35.0 37.0 6 36.17200974457037 37.0 37.0 37.0 35.0 37.0 7 36.122264481409054 37.0 36.0 37.0 35.0 37.0 8 36.10072456433858 37.0 36.0 37.0 35.0 37.0 9 37.8710960693183 39.0 38.0 39.0 35.0 39.0 10-11 37.802350409424584 39.0 38.0 39.0 35.0 39.0 12-13 37.74149068544858 39.0 38.0 39.0 35.0 39.0 14-15 39.08409400771707 40.0 38.0 41.0 36.0 41.0 16-17 39.01087008881285 40.0 38.0 41.0 35.5 41.0 18-19 39.024883456475536 40.0 38.5 41.0 35.0 41.0 20-21 38.97491059989943 40.0 38.0 41.0 35.0 41.0 22-23 38.85276979291435 40.0 38.0 41.0 35.0 41.0 24-25 39.017282756537824 40.0 39.0 41.0 35.0 41.0 26-27 38.93975540067537 40.0 38.0 41.0 35.0 41.0 28-29 38.84928147054032 40.0 38.0 41.0 35.0 41.0 30-31 38.74287275390196 40.0 38.0 41.0 35.0 41.0 32-33 38.59413139149152 40.0 38.0 41.0 34.0 41.0 34-35 38.461109670798706 40.0 38.0 41.0 34.0 41.0 36-37 38.32202844457627 40.0 38.0 41.0 34.0 41.0 38-39 38.19335697851204 40.0 38.0 41.0 33.5 41.0 40-41 38.05541128376217 40.0 38.0 41.0 33.0 41.0 42-43 38.089053162682475 40.0 38.0 41.0 33.0 41.0 44-45 37.96520093981738 40.0 38.0 41.0 33.0 41.0 46-47 37.77594551397412 40.0 37.5 41.0 33.0 41.0 48-49 37.56017153128421 40.0 37.0 41.0 33.0 41.0 50-51 37.367826951065524 40.0 37.0 41.0 32.0 41.0 52-53 37.15863342197118 40.0 36.5 41.0 32.0 41.0 54-55 36.971420383168805 39.5 36.0 41.0 31.0 41.0 56-57 36.72057130547691 39.0 35.5 41.0 31.0 41.0 58-59 36.45160595434167 39.0 35.0 41.0 31.0 41.0 60-61 36.165468260596356 38.5 35.0 40.5 30.0 41.0 62-63 35.8600741613548 38.0 35.0 40.0 30.0 41.0 64-65 35.48696953279212 37.5 35.0 40.0 29.0 41.0 66-67 35.11852935143183 37.0 34.0 40.0 29.0 41.0 68-69 34.75175187392462 36.5 34.0 39.0 28.5 41.0 70-71 34.36740667452193 36.0 34.0 39.0 28.0 40.5 72-73 33.94844021383497 35.5 34.0 38.5 28.0 40.0 74-75 33.50241043346668 35.0 33.0 37.0 27.0 39.5 76-77 32.77936753386704 35.0 32.5 37.0 26.0 39.0 78-79 32.83277377647566 35.0 33.0 36.0 26.0 39.0 80-81 32.655974557167085 35.0 33.0 36.0 26.0 37.5 82-83 32.3579029580649 35.0 33.0 36.0 26.0 37.0 84-85 32.051606333212995 35.0 33.0 35.0 26.0 37.0 86-87 31.637991159308356 35.0 32.0 35.0 25.0 36.0 88-89 31.136373649391395 34.5 32.0 35.0 23.5 36.0 90-91 30.89949761662877 34.0 31.5 35.0 21.5 36.0 92-93 30.59341992527575 34.0 31.0 35.0 19.5 35.0 94-95 30.412807636746837 34.0 31.0 35.0 19.0 35.0 96-97 30.13334403193777 34.0 31.0 35.0 14.5 35.0 98-99 29.6510595136483 34.0 31.0 35.0 2.0 35.0 100-101 26.196801189439448 30.5 24.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1330072300553624 1101 2 0.16619578606673002 1101 3 0.22549943943479178 1101 4 0.19356719183845428 1101 5 0.15433883229226808 1101 6 0.2572511507500792 1101 7 0.19737698990241626 1101 8 0.15143591567817793 1101 9 0.1772288290731936 1101 10-11 0.12304426945761548 1101 12-13 0.28606716603924554 1101 14-15 0.38415528834344315 1101 16-17 0.44356207177377627 1101 18-19 0.42629708896557617 1101 20-21 0.2293376020671971 1101 22-23 0.30632697517052065 1101 24-25 0.2336948977541482 1101 26-27 0.22507657501711975 1101 28-29 0.2794606370192767 1101 30-31 0.3652040648175259 1101 32-33 0.3952384345197757 1101 34-35 0.3371988839321389 1101 36-37 0.3454165910507925 1101 38-39 0.37529941613441054 1101 40-41 0.4116791965389055 1101 42-43 0.4253162740686989 1101 44-45 0.46123662774327556 1101 46-47 0.4057896759812323 1101 48-49 0.36432867271544467 1101 50-51 0.4313484962204015 1101 52-53 0.486966616780407 1101 54-55 0.4154236506030813 1101 56-57 0.4284033526121718 1101 58-59 0.35085304439964915 1101 60-61 0.5084525793567778 1101 62-63 0.3745484139973314 1101 64-65 0.4020969492497599 1101 66-67 0.40304792216091556 1101 68-69 0.4345823672889537 1101 70-71 0.507410026103166 1101 72-73 0.45012761380746014 1101 74-75 0.42045676678687727 1101 76-77 0.3741929789678693 1101 78-79 0.3416283436333387 1101 80-81 0.42779871353107524 1101 82-83 0.4132999785734839 1101 84-85 0.5302794568035125 1101 86-87 0.48689015510803557 1101 88-89 0.36368396033949324 1101 90-91 0.6681727903934238 1101 92-93 0.7104303180242368 1101 94-95 0.7804551998754903 1101 96-97 0.7239543829303869 1101 98-99 0.7357595975592801 1101 100-101 0.9634116324094713 1102 1 0.12953707904592449 1102 2 0.17925296444122552 1102 3 0.15102115738152122 1102 4 0.14932760456824212 1102 5 0.1432214474618192 1102 6 0.1761293034837763 1102 7 0.1115851428030794 1102 8 0.12587405098712168 1102 9 0.06563795551343077 1102 10-11 0.21442213000801047 1102 12-13 0.19939914849913976 1102 14-15 0.2138000921101053 1102 16-17 0.18573933824342248 1102 18-19 0.27353360413709993 1102 20-21 0.26087328713457936 1102 22-23 0.30024083498021525 1102 24-25 0.25378534556109855 1102 26-27 0.20885281269798384 1102 28-29 0.20355388219672932 1102 30-31 0.23738961866803976 1102 32-33 0.258932458650321 1102 34-35 0.3474899971581067 1102 36-37 0.23946409626076104 1102 38-39 0.40033098710915027 1102 40-41 0.325554184585954 1102 42-43 0.3987583299462827 1102 44-45 0.45941807129573675 1102 46-47 0.4506124914746863 1102 48-49 0.38813383274840874 1102 50-51 0.3491899674278187 1102 52-53 0.36995479436107104 1102 54-55 0.30963337446330996 1102 56-57 0.4134646276241938 1102 58-59 0.3627822813990065 1102 60-61 0.32816713524575647 1102 62-63 0.31920377430866864 1102 64-65 0.3009542848719917 1102 66-67 0.28610629962530965 1102 68-69 0.17156484236230085 1102 70-71 0.2797933538964017 1102 72-73 0.16172334444025438 1102 74-75 0.17237891549527973 1102 76-77 0.10158785748792809 1102 78-79 0.21526760426539937 1102 80-81 0.24296773208358502 1102 82-83 0.297075153769633 1102 84-85 0.22671789416169474 1102 86-87 0.28391440100950405 1102 88-89 0.31317552345184296 1102 90-91 0.3784459172707919 1102 92-93 0.36845386116794643 1102 94-95 0.2987396133763305 1102 96-97 0.2694427692134056 1102 98-99 0.3152364907932288 1102 100-101 0.6226456492240509 1103 1 0.04101216760596316 1103 2 0.03104228398951392 1103 3 0.1237487326483091 1103 4 -0.002854411272728896 1103 5 0.08468751362116222 1103 6 0.047030951920760344 1103 7 0.12115554341245627 1103 8 0.11843130995221429 1103 9 0.171444461995506 1103 10-11 0.1645515496901595 1103 12-13 0.1650759394826551 1103 14-15 0.3129908493261766 1103 16-17 0.3108295622504116 1103 18-19 0.13868476632584503 1103 20-21 0.12575655058128632 1103 22-23 0.288575684372681 1103 24-25 0.25512280553343913 1103 26-27 0.2114770740813654 1103 28-29 0.1718883987957298 1103 30-31 0.25318935834959433 1103 32-33 0.1924338526851841 1103 34-35 0.2147595746583022 1103 36-37 0.21297077801406772 1103 38-39 0.15192303408479546 1103 40-41 0.33472375395120935 1103 42-43 0.3224506365008395 1103 44-45 0.3004675909638337 1103 46-47 0.3993907696752359 1103 48-49 0.43891090549705325 1103 50-51 0.4669718619840637 1103 52-53 0.4699241034078767 1103 54-55 0.3091428738679767 1103 56-57 0.2854055161088027 1103 58-59 0.3613533032228702 1103 60-61 0.32480887937934 1103 62-63 0.3424861902413383 1103 64-65 0.4214356891125206 1103 66-67 0.2952699526622382 1103 68-69 0.3658176840487499 1103 70-71 0.1429059712060976 1103 72-73 0.2671157079394746 1103 74-75 0.32150533264079684 1103 76-77 0.2952718072287155 1103 78-79 0.324348243820495 1103 80-81 0.3326884813865618 1103 82-83 0.4146033857488689 1103 84-85 0.40770078118200814 1103 86-87 0.20037418106999638 1103 88-89 0.38370758625367074 1103 90-91 0.5366202492803502 1103 92-93 0.8061484529043135 1103 94-95 0.6168404350064272 1103 96-97 0.6251091326682428 1103 98-99 0.48392062541928027 1103 100-101 0.6728606707816702 1104 1 0.07423957849294993 1104 2 0.08261185104060331 1104 3 0.05104036598697803 1104 4 0.046155565312837155 1104 5 0.11566582981784279 1104 6 0.09941275265082083 1104 7 0.02415251844190891 1104 8 0.05780428300379725 1104 9 -0.04055361831164106 1104 10-11 0.050822291825966204 1104 12-13 0.17372510107134786 1104 14-15 0.2483253776198282 1104 16-17 0.20556931298119707 1104 18-19 0.2842169357241957 1104 20-21 0.22350672873460553 1104 22-23 0.2148360281722148 1104 24-25 0.19194527757427693 1104 26-27 0.3227125491326319 1104 28-29 0.3264533047744109 1104 30-31 0.18609536611562305 1104 32-33 0.14892899528054926 1104 34-35 0.03943780795916041 1104 36-37 0.1373216091538083 1104 38-39 0.19359036731147938 1104 40-41 0.3727134937746541 1104 42-43 0.2745164877828117 1104 44-45 0.3324688344012401 1104 46-47 0.25009065769314986 1104 48-49 0.17269840858747187 1104 50-51 0.23437780430602118 1104 52-53 0.2249705687007051 1104 54-55 0.16214984729767679 1104 56-57 0.1258434096821972 1104 58-59 0.25134763140308536 1104 60-61 0.12859700055268064 1104 62-63 0.06046458642522623 1104 64-65 0.05626138972769468 1104 66-67 -0.015282540756992091 1104 68-69 0.09488176106915347 1104 70-71 0.11978567045421329 1104 72-73 -0.023161665862538428 1104 74-75 -0.09211703323520481 1104 76-77 0.0014579083616226285 1104 78-79 0.024331155645505476 1104 80-81 0.14101660343249023 1104 82-83 0.12802632898704047 1104 84-85 0.22676082248449347 1104 86-87 0.22478609334584831 1104 88-89 0.20205519609064027 1104 90-91 0.41074838877802833 1104 92-93 0.46367688471886126 1104 94-95 0.43942373077001307 1104 96-97 0.35197428878872117 1104 98-99 0.23843699370944194 1104 100-101 0.49310252299440904 1105 1 0.03560720443049803 1105 2 0.057879642440482826 1105 3 0.11058580653392625 1105 4 0.05625443675336328 1105 5 0.026930706728990117 1105 6 0.06855689805609444 1105 7 0.18023059930287388 1105 8 0.06337253831412681 1105 9 0.11792625603777651 1105 10-11 0.1653221882370275 1105 12-13 0.1034312584537389 1105 14-15 0.11431016544449335 1105 16-17 0.20901154535078348 1105 18-19 0.1104487691643854 1105 20-21 0.07751632051019897 1105 22-23 -0.019125009788929503 1105 24-25 0.06508312645202352 1105 26-27 0.07315892645953426 1105 28-29 -0.09096444001044546 1105 30-31 -0.022184889553066967 1105 32-33 0.024372222943839006 1105 34-35 0.09781157364098192 1105 36-37 0.10470806728007886 1105 38-39 0.17270057334147282 1105 40-41 0.2011868378215027 1105 42-43 0.15083549691264153 1105 44-45 0.16054147124363283 1105 46-47 0.20375315653827641 1105 48-49 0.29880586531109543 1105 50-51 0.15336145685192548 1105 52-53 0.20858461872015965 1105 54-55 0.1543105095389521 1105 56-57 0.15499739603644969 1105 58-59 0.3725578861478738 1105 60-61 0.39833594243368964 1105 62-63 0.3864783931506679 1105 64-65 0.23949971987403273 1105 66-67 0.20174328088525328 1105 68-69 0.23855436683485465 1105 70-71 0.18021266685099846 1105 72-73 0.3580828922186612 1105 74-75 0.2945802924904015 1105 76-77 0.31203626359328496 1105 78-79 0.3003743242351007 1105 80-81 0.3520321669703037 1105 82-83 0.3056803055423458 1105 84-85 0.44268933631921925 1105 86-87 0.3415282002888276 1105 88-89 0.3902035018515839 1105 90-91 0.5330921724619309 1105 92-93 0.5802702575466405 1105 94-95 0.39992764895155375 1105 96-97 0.37220807782005494 1105 98-99 0.5554653350825873 1105 100-101 0.5437932092291184 1106 1 0.032801946357643885 1106 2 0.06163710974762182 1106 3 0.0279987503199024 1106 4 -0.01043729103522395 1106 5 -0.009634604142320313 1106 6 0.07794563347868433 1106 7 0.05431274988733037 1106 8 0.0191929555307766 1106 9 -0.013472834957909186 1106 10-11 0.07092215829975856 1106 12-13 0.08481333963816695 1106 14-15 0.20933989859457114 1106 16-17 0.11264620182063112 1106 18-19 0.16405586974000386 1106 20-21 0.1577951453363582 1106 22-23 0.08635878326290225 1106 24-25 0.07687211634139146 1106 26-27 -0.010865118609665103 1106 28-29 0.00976392010767313 1106 30-31 0.13871720877724414 1106 32-33 0.039365180881972606 1106 34-35 -0.026613154542232564 1106 36-37 -0.010939768391537541 1106 38-39 0.24715523879250867 1106 40-41 0.15978648809978324 1106 42-43 0.14789330985914972 1106 44-45 0.04576164624056389 1106 46-47 0.02024059649364318 1106 48-49 0.03531385311846691 1106 50-51 -0.06296558058326696 1106 52-53 -0.04652019573682509 1106 54-55 -0.08183312811124921 1106 56-57 0.08645874294134615 1106 58-59 0.07606667001441281 1106 60-61 0.07854553732801861 1106 62-63 -0.017559225747916685 1106 64-65 0.05158519077364332 1106 66-67 0.04868763839066048 1106 68-69 0.15324523052503736 1106 70-71 0.1046999911396469 1106 72-73 0.0533822308771974 1106 74-75 0.314404887316698 1106 76-77 0.2090689020191263 1106 78-79 0.020942928205869293 1106 80-81 0.10453056804804817 1106 82-83 0.2481679223997446 1106 84-85 0.38019494955692323 1106 86-87 0.3715898098252097 1106 88-89 0.37766301850078676 1106 90-91 0.5885941660118128 1106 92-93 0.5309991279590953 1106 94-95 0.5338935204662434 1106 96-97 0.5019478617274089 1106 98-99 0.3939519041356121 1106 100-101 0.7103677394181318 1107 1 -0.048129431807097944 1107 2 0.058028448961735535 1107 3 0.027414490954569715 1107 4 0.041438639770134955 1107 5 -0.03857262682533502 1107 6 -0.00469842655110142 1107 7 0.051322716664735424 1107 8 0.030964063332362457 1107 9 0.004040625919621732 1107 10-11 0.08803179285125395 1107 12-13 0.030208001111418525 1107 14-15 0.10026812634116311 1107 16-17 0.10878837159953036 1107 18-19 0.05095156225918629 1107 20-21 -0.08436031347908113 1107 22-23 0.057188775243659506 1107 24-25 0.009325719274137612 1107 26-27 0.11874308393358746 1107 28-29 0.0633410767120921 1107 30-31 0.0028998184690820494 1107 32-33 -0.01705021607071444 1107 34-35 0.003174049032061532 1107 36-37 0.14440878241611443 1107 38-39 0.09960540490546066 1107 40-41 0.025567218996798147 1107 42-43 -0.2483949247610866 1107 44-45 -0.05845705005912549 1107 46-47 -0.03849178980490109 1107 48-49 -0.06449282576801352 1107 50-51 0.07916267007545486 1107 52-53 0.01959132906556249 1107 54-55 0.14161457494753193 1107 56-57 -0.10276396298144164 1107 58-59 -0.19385972476162294 1107 60-61 0.0667672694852044 1107 62-63 -0.07457177014016736 1107 64-65 0.13664504871842809 1107 66-67 0.03396086556445255 1107 68-69 0.07463227371440695 1107 70-71 0.3183370056888535 1107 72-73 0.1002031725658199 1107 74-75 0.0376750213526762 1107 76-77 0.03249798897067535 1107 78-79 -0.030885746327300012 1107 80-81 0.20582939952931412 1107 82-83 0.34191577438736687 1107 84-85 0.12706457952599237 1107 86-87 0.07946442318298352 1107 88-89 0.27622671422767553 1107 90-91 0.07614571830875505 1107 92-93 0.26829864680432536 1107 94-95 0.2922636086779491 1107 96-97 0.10261804158764676 1107 98-99 0.17810100483443492 1107 100-101 0.153080249442187 1108 1 -0.10056401297730844 1108 2 -0.05211997009472924 1108 3 -0.048256698222367334 1108 4 -0.03688980062727154 1108 5 -0.06541397406898142 1108 6 -0.11097327133169443 1108 7 -0.08001045633510273 1108 8 0.021173353829006203 1108 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66-67 -0.7285686294310665 2316 68-69 -0.6041058316253114 2316 70-71 -0.6288438318122687 2316 72-73 -0.6120898660927381 2316 74-75 -0.6695227502991088 2316 76-77 -0.4830538940065594 2316 78-79 -0.761590297909315 2316 80-81 -0.8189240599280012 2316 82-83 -0.8025852768780126 2316 84-85 -0.7072602802878691 2316 86-87 -0.7784830804274776 2316 88-89 -0.7137661484536579 2316 90-91 -0.7699200568374955 2316 92-93 -0.6506716251661793 2316 94-95 -0.5876913676947026 2316 96-97 -0.5325331744735138 2316 98-99 -0.5884159314062174 2316 100-101 -0.5203558104235064 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 16.0 5 29.0 6 46.0 7 142.0 8 269.0 9 606.0 10 888.0 11 1454.0 12 2225.0 13 3306.0 14 4106.0 15 5508.0 16 7043.0 17 9395.0 18 8887.0 19 6454.0 20 5846.0 21 6317.0 22 6768.0 23 8013.0 24 9250.0 25 11171.0 26 14113.0 27 17746.0 28 21748.0 29 26819.0 30 33720.0 31 41564.0 32 52546.0 33 67922.0 34 94825.0 35 137766.0 36 232257.0 37 434699.0 38 484520.0 39 89546.0 40 63.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.37567999479066 18.04986311925311 13.429778413268615 24.144678472687612 2 15.718234496736724 19.092595159006162 40.55057751523367 24.63859282902344 3 20.232107395947054 23.075807280066552 28.460218240705608 28.231867083280786 4 13.136984173462446 15.182348060422399 35.914673848623586 35.76599391749157 5 14.203615190141985 37.04333151294684 33.75884190944651 14.994211387464665 6 34.413369178168566 34.76479939034192 16.52988509915333 14.291946332336181 7 30.482525101578105 31.20773893384528 19.496609913546976 18.813126051029638 8 26.905384465085113 36.183185366041116 18.3899267858235 18.521503383050273 9 26.640986407720746 16.347918616275336 19.263658175799538 37.747436800204376 10-11 26.04120942613257 25.948223277171795 26.508548717868223 21.50201857882741 12-13 26.319697833639093 22.788289510966035 27.49403750774995 23.39797514764492 14-15 23.029287294333763 24.481961124555028 25.287603925756375 27.20114765535483 16-17 23.100238392860284 27.806826279017343 25.79722507354889 23.295710254573482 18-19 22.980791765285968 26.71421682156189 27.70531713423898 22.59967427891316 20-21 23.650771571444576 25.84790048457642 27.867636432915692 22.633691511063315 22-23 23.242462826624376 25.75413680235225 27.852434993105895 23.15096537791748 24-25 22.935963049453857 25.933797208790775 27.951387526349077 23.17885221540629 26-27 23.024485663585377 26.137147536697913 27.855206122917885 22.98316067679882 28-29 22.94049098482749 26.29953040563946 27.781383149161705 22.978595460371345 30-31 23.23279505015097 26.0693293872524 27.677332108662643 23.02054345393399 32-33 22.937902370496545 26.128342335715654 27.886005726992774 23.047749566795027 34-35 23.17600364588182 26.149324191205288 27.781283895984117 22.893388266928778 36-37 23.180157296717727 26.146966509362734 27.744262664193535 22.928613529726004 38-39 23.090987128644812 26.398510276354454 27.703777513529783 22.80672508147095 40-41 23.358018811118882 26.2480193658994 27.568884119881304 22.825077703100412 42-43 23.073279948384986 26.192264297442335 27.536901405096387 23.19755434907629 44-45 23.167695660634156 26.29500196615294 27.44787394320971 23.089428430003196 46-47 23.09574655244746 26.406100713193403 27.221306483699404 23.276846250659737 48-49 23.25557224635622 26.433275141082373 27.194916974544274 23.116235638017134 50-51 23.112105851436677 26.604104476602103 27.087001588637587 23.196788083323636 52-53 23.174893319775986 26.7007061855991 27.012674966528376 23.111725528096542 54-55 23.129161677051833 26.359807045674426 27.200281448104626 23.31074982916912 56-57 23.17739385439935 26.54266527309661 27.128744114448832 23.1511967580552 58-59 23.281360449369604 26.657613313949273 27.089478806169847 22.971547430511276 60-61 23.341641566061273 26.494548229696925 27.225726835050455 22.938083369191343 62-63 23.27701501358795 26.525863650706622 27.13468280081165 23.06243853489378 64-65 23.16788420726173 26.484579533085768 27.14576983800946 23.20176642164305 66-67 23.145059545040496 26.61143444470725 27.00776090838188 23.23574510187038 68-69 22.954703768633028 26.513063212514876 27.234190646966077 23.298042371886016 70-71 23.093462294123697 26.418090593692323 27.09218495445418 23.3962621577298 72-73 23.18928121704269 26.59598968477622 26.965016842479585 23.249712255701503 74-75 22.943527064672793 26.7437417223382 26.93986175526753 23.372869457721478 76-77 23.056713290581186 26.657567465856307 26.92566310832604 23.360056135236466 78-79 23.11485654952224 26.56533972931746 26.909943781401672 23.40985993975863 80-81 23.133221618906585 26.821854459484033 26.73977358343509 23.30515033817429 82-83 23.137211496254857 26.79700020513176 26.91426087888404 23.151527419729344 84-85 23.300048387568182 26.71938241442058 26.708882203875117 23.27168699413612 86-87 22.977316465930265 26.726151076709908 26.962033170343663 23.33449928701616 88-89 23.071969043278113 26.659750980871443 26.83673519383609 23.431544782014356 90-91 23.101817317120048 26.734524247724963 26.867074279050524 23.29658415610447 92-93 23.177430732447558 26.728754119086208 26.84226054202438 23.251554606441857 94-95 23.01895044047379 26.716077888843426 26.880294832741196 23.384676837941583 96-97 22.974980758176557 26.670818137434793 26.80951623187182 23.54468487251683 98-99 22.951542386710997 26.582636123390934 26.753538822516937 23.712282667381128 100-101 22.953932736913835 26.76870459265385 26.672172965623698 23.605189704808613 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 79.0 1 73.5 2 134.0 3 226.5 4 213.0 5 184.0 6 359.0 7 779.0 8 1275.0 9 1483.5 10 1366.5 11 1228.5 12 1228.0 13 1293.0 14 1364.0 15 1444.0 16 1537.0 17 1616.0 18 1705.0 19 1821.0 20 2008.5 21 2466.0 22 3017.5 23 3783.0 24 4776.0 25 5893.0 26 7388.5 27 9289.5 28 11069.0 29 13022.5 30 15747.0 31 18630.0 32 21507.5 33 25146.0 34 29380.5 35 32815.5 36 36913.5 37 40914.5 38 43370.5 39 46077.0 40 49293.5 41 52259.0 42 55362.5 43 59714.0 44 64057.5 45 67451.0 46 69535.0 47 71841.5 48 75053.5 49 75797.0 50 74524.5 51 72955.5 52 71828.5 53 71281.0 54 70338.0 55 66710.5 56 61067.0 57 56254.0 58 52171.0 59 46581.0 60 38724.5 61 31470.0 62 25102.5 63 19334.0 64 14579.5 65 10678.0 66 7993.5 67 6534.5 68 5141.0 69 3528.5 70 2332.0 71 1612.0 72 1185.5 73 908.5 74 647.0 75 427.0 76 314.0 77 201.0 78 104.0 79 48.5 80 28.0 81 14.0 82 8.0 83 5.5 84 4.5 85 3.0 86 0.5 87 0.5 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25698300437379873 2 0.019971931047584615 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.2474684630218886E-4 12-13 8.118671157554722E-5 14-15 0.0 16-17 6.765559297962268E-4 18-19 0.0 20-21 0.0 22-23 2.706223719184907E-5 24-25 0.0023002901613071707 26-27 0.0023002901613071707 28-29 0.0022191034497316235 30-31 0.00116367619924951 32-33 0.0014342985711680006 34-35 0.001569609757127246 36-37 1.0824894876739628E-4 38-39 1.0824894876739628E-4 40-41 8.389293529473211E-4 42-43 0.0037075264952833227 44-45 0.004140522290352907 46-47 0.014505359134831102 48-49 0.014505359134831102 50-51 0.005304198489602417 52-53 0.007171492855840004 54-55 2.976846091103398E-4 56-57 0.0032474684630218885 58-59 0.0014613608083598499 60-61 0.001515485282743548 62-63 0.0 64-65 8.659915901391703E-4 66-67 0.0 68-69 0.0 70-71 1.6237342315109443E-4 72-73 0.0017590454174701896 74-75 0.0 76-77 0.18808254848335104 78-79 0.1002114643214171 80-81 0.0013531118595924536 82-83 0.0 84-85 5.953692182206796E-4 86-87 0.1179642919192701 88-89 0.5794566227518723 90-91 0.3056409068447434 92-93 0.292623970755464 94-95 0.002002605552196831 96-97 0.0029768460911033974 98-99 0.0027332859563767562 100-101 6.494936926043777E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1847593.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.726766529632904 #Duplication Level Percentage of deduplicated Percentage of total 1 75.74462991829363 45.99726456921665 2 14.017534741260086 17.02479119107039 3 4.6004048765024015 8.38103138631438 4 2.076595845174292 5.044198042652198 5 1.0866495062349886 3.2994355432336517 6 0.6101801215554147 2.2232559469631217 7 0.40503092049147943 1.7217352702177866 8 0.2629959718036763 1.2776715982364615 9 0.20458561260836916 1.118144045897132 >10 0.9298374075904885 9.793345682408312 >50 0.04531102635758772 1.8553640181624562 >100 0.015530377077353576 1.5151325768589285 >500 4.460469064785016E-4 0.19778917433489196 >1k 2.6762814388709967E-4 0.5508409544336277 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4570 0.2473488479335005 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3300 0.17861076546620386 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2298 0.12437804213373832 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 2.706223719184907E-5 0.0 0.0 0.0 14-15 0.0 1.0824894876739628E-4 0.0 2.706223719184907E-5 0.0 16-17 0.0 1.894356603429435E-4 0.0 8.118671157554722E-5 0.0 18-19 0.0 2.1649789753479257E-4 0.0 1.6237342315109443E-4 0.0 20-21 0.0 2.1649789753479257E-4 0.0 2.706223719184907E-4 0.0 22-23 0.0 2.1649789753479257E-4 0.0 5.412447438369815E-4 0.0 24-25 0.0 2.1649789753479257E-4 0.0 0.0010013027760984157 0.0 26-27 0.0 2.1649789753479257E-4 0.0 0.0012448629108250573 0.0 28-29 0.0 2.4356013472664166E-4 0.0 0.0016778587058946424 0.0 30-31 0.0 2.976846091103398E-4 0.0 0.0028956593795278506 0.0 32-33 0.0 3.2474684630218886E-4 0.0 0.004979451643300229 0.0 34-35 0.0 4.0593355787773606E-4 0.0 0.007387990753374797 0.0 36-37 0.0 5.141825066451324E-4 0.0 0.013368745172773443 0.0 38-39 0.0 6.224314554125287E-4 0.0 0.023219399510606503 0.0 40-41 0.0 7.036181669880759E-4 0.0 0.03371954754104394 0.0 42-43 0.0 7.036181669880759E-4 0.0 0.04508568716162055 0.0 44-45 0.0 7.036181669880759E-4 0.0 0.057804938641789615 0.0 46-47 0.0 7.036181669880759E-4 0.0 0.07379872082217241 0.0 48-49 0.0 7.036181669880759E-4 0.0 0.09184923302913575 0.0 50-51 0.0 7.036181669880759E-4 0.0 0.11149641723041817 0.0 52-53 0.0 7.036181669880759E-4 0.0 0.13393101186246104 0.0 54-55 0.0 7.036181669880759E-4 0.0 0.15742103374498606 0.0 56-57 0.0 7.036181669880759E-4 0.0 0.18548457371293353 0.0 58-59 0.0 7.306804041799249E-4 0.0 0.2197724282350063 0.0 60-61 0.0 9.471783017147176E-4 0.0 0.25503452329598564 0.0 62-63 0.0 0.0010283650132902648 0.0 0.29056724072888346 0.0 64-65 0.0 0.0010283650132902648 0.0 0.3307817251959712 0.0 66-67 0.0 0.0011907384364413592 0.0 0.3771934619799924 0.0 68-69 0.0 0.0011907384364413592 0.0 0.43028957135040025 0.0 70-71 2.706223719184907E-5 0.0012178006736332082 0.0 0.49012417778157846 0.0 72-73 5.412447438369814E-5 0.0012448629108250573 0.0 0.5527732568807091 0.0 74-75 5.412447438369814E-5 0.0012989873852087554 0.0 0.6173708170576528 0.0 76-77 5.412447438369814E-5 0.0014072363339761517 0.0 0.6877596959936523 0.0 78-79 5.412447438369814E-5 0.0014072363339761517 0.0 0.7743859172447611 0.0 80-81 5.412447438369814E-5 0.0014342985711680008 0.0 0.8718099711354179 0.0 82-83 5.412447438369814E-5 0.001488423045551699 0.0 0.9699647054302544 0.0 84-85 5.412447438369814E-5 0.0015696097571272461 0.0 1.0745331899395594 0.0 86-87 5.412447438369814E-5 0.0016778587058946424 0.0 1.1865979141510063 0.0 88-89 5.412447438369814E-5 0.0017319831802783405 0.0 1.3144940471196849 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5490 0.0 34.05483 1 GGTATCA 3685 0.0 32.40371 1 GTACCTG 1635 0.0 28.805534 1 ATCAACG 6575 0.0 28.065739 3 TCAACGC 6610 0.0 27.91713 4 GTACATG 6255 0.0 27.608181 1 CAACGCA 6770 0.0 27.257347 5 TATCAAC 6840 0.0 26.846956 2 TACATGG 6385 0.0 26.381996 2 AACGCAG 7060 0.0 26.204903 6 ACATGGG 6535 0.0 23.954716 3 TACCTGG 1785 0.0 23.92459 2 GTGTAGC 1145 0.0 22.020584 1 ACGCAGA 8405 0.0 21.842175 7 CATGGGG 3995 0.0 21.729862 4 CGCAGAG 8505 0.0 21.473806 8 TAACGCA 145 6.2026456E-4 19.629349 4 AGAGTAC 7450 0.0 19.389446 10-11 GCAGAGT 9345 0.0 19.239 9 GAGTACT 4460 0.0 19.092552 12-13 >>END_MODULE