FastQCFastQC Report
Thu 26 May 2016
SRR936302_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936302_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259029
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49750.22022736317240726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35260.15608476031073526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23920.10588620154942675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29850.048.843821
GTATCAA54200.043.145041
ATCAACG66950.034.500423
TCAACGC66950.034.428964
CAACGCA68100.033.917065
TATCAAC71450.032.3930742
AACGCAG72050.032.1211856
GTACATG88150.027.0115131
ACGCAGA86750.026.4059727
CGCAGAG87400.026.210758
TACATGG88000.025.81692
ACATGGG89500.024.4328483
TACCTGG18600.023.4110222
GCAGAGT101150.022.2729119
GTACCTG21100.022.2103791
GTGGTAT18900.021.5397111
TGGTATC17000.021.438152
GTGTAGG11200.020.7100561
CATGGGG55150.020.4257014
GAGTACA67950.019.7151571
Produced by FastQC (version 0.11.5)