##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936302_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2259029 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.6777544688448 33.0 31.0 34.0 30.0 34.0 2 31.770133539675676 33.0 31.0 34.0 30.0 34.0 3 31.837014929865884 34.0 31.0 34.0 30.0 34.0 4 35.33177396129045 37.0 35.0 37.0 33.0 37.0 5 35.31310576358249 37.0 35.0 37.0 33.0 37.0 6 35.403791629058325 37.0 35.0 37.0 33.0 37.0 7 35.34920711509237 37.0 35.0 37.0 33.0 37.0 8 35.3490380158909 37.0 35.0 37.0 33.0 37.0 9 37.006185400895696 39.0 37.0 39.0 33.0 39.0 10-11 36.92298970044209 39.0 37.0 39.0 33.0 39.0 12-13 36.82522313790571 39.0 37.0 39.0 33.0 39.0 14-15 38.05832505912939 40.0 38.0 41.0 33.0 41.0 16-17 38.00084616000946 40.0 38.0 41.0 33.0 41.0 18-19 37.946905728080516 40.0 38.0 41.0 33.0 41.0 20-21 37.86642468954582 40.0 38.0 41.0 33.0 41.0 22-23 37.702374117375214 40.0 38.0 41.0 32.0 41.0 24-25 37.86696452325313 40.0 38.0 41.0 33.0 41.0 26-27 37.81787462666482 40.0 38.0 41.0 33.0 41.0 28-29 37.68772180436816 40.0 38.0 41.0 32.0 41.0 30-31 37.533161371545035 40.0 37.5 41.0 32.0 41.0 32-33 37.35078146407151 40.0 37.0 41.0 31.5 41.0 34-35 37.08539664608113 40.0 37.0 41.0 31.0 41.0 36-37 36.882504828401935 40.0 37.0 41.0 30.5 41.0 38-39 36.72421624512124 40.0 36.5 41.0 30.0 41.0 40-41 36.53580432123714 40.0 36.0 41.0 30.0 41.0 42-43 36.34106910535456 40.0 36.0 41.0 30.0 41.0 44-45 36.1534502213119 40.0 35.5 41.0 29.0 41.0 46-47 35.874652339567135 39.0 35.0 41.0 28.5 41.0 48-49 35.69475402927541 39.0 35.0 41.0 27.5 41.0 50-51 35.38750277220876 38.5 35.0 40.5 27.5 41.0 52-53 35.40258757191696 39.0 35.0 40.5 27.0 41.0 54-55 35.47865166848234 39.0 35.0 41.0 27.0 41.0 56-57 35.313977377005784 39.0 35.0 41.0 27.0 41.0 58-59 35.06778974506304 39.0 35.0 41.0 26.0 41.0 60-61 34.77043875930765 38.0 34.5 40.0 26.0 41.0 62-63 34.45985996638379 37.5 34.0 40.0 26.0 41.0 64-65 34.11877713831916 37.0 34.0 40.0 25.5 41.0 66-67 33.759776656253635 36.5 34.0 39.5 24.5 41.0 68-69 33.37437921336999 36.0 33.5 39.0 23.0 41.0 70-71 32.96791984520783 35.5 33.0 39.0 22.5 40.5 72-73 32.562007614776086 35.0 33.0 38.0 21.5 40.0 74-75 32.14257453091572 35.0 32.5 37.0 20.0 39.5 76-77 31.744140292134365 35.0 32.0 37.0 19.0 39.0 78-79 31.351344316518293 35.0 32.0 36.0 18.0 39.0 80-81 30.957332110389018 35.0 31.5 36.0 15.0 37.5 82-83 30.605941313723726 35.0 31.0 35.0 9.5 37.0 84-85 30.287926582615803 34.5 31.0 35.0 7.0 37.0 86-87 29.966414331113057 34.0 31.0 35.0 3.5 36.0 88-89 29.641610399866494 34.0 30.5 35.0 2.0 36.0 90-91 29.284942999846393 34.0 30.0 35.0 2.0 35.5 92-93 28.960412637465033 34.0 29.5 35.0 2.0 35.0 94-95 28.572950369384365 34.0 29.0 35.0 2.0 35.0 96-97 28.13187944909074 34.0 29.0 35.0 2.0 35.0 98-99 27.61860449777316 34.0 29.0 35.0 2.0 35.0 100-101 24.30360610687158 30.0 14.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2779691038182932 1101 2 0.16346584720588808 1101 3 0.26542066600991276 1101 4 0.0802107385498303 1101 5 0.3096724077821733 1101 6 0.16988899204633157 1101 7 0.30820779561868505 1101 8 0.27059718284270673 1101 9 0.25725989949958716 1101 10-11 0.5493721072802984 1101 12-13 0.46414054855655706 1101 14-15 0.5191630878960538 1101 16-17 0.5649209170963942 1101 18-19 0.5892914003901168 1101 20-21 0.6509584798889563 1101 22-23 0.6545248913359956 1101 24-25 0.5018182463791518 1101 26-27 0.6579125149293077 1101 28-29 0.6154313763003358 1101 30-31 0.5956637111546428 1101 32-33 0.5944437503879669 1101 34-35 0.45118453045892437 1101 36-37 0.39040740332011836 1101 38-39 0.37648146488199785 1101 40-41 0.3167717369530081 1101 42-43 0.22398999242465578 1101 44-45 0.274343340964208 1101 46-47 0.16061569491288452 1101 48-49 0.03727947830206091 1101 50-51 0.07791987487919982 1101 52-53 0.22587796041039354 1101 54-55 0.2379650610139734 1101 56-57 0.19944209227825382 1101 58-59 0.32336546469664995 1101 60-61 0.17049384002954326 1101 62-63 0.1558402693758083 1101 64-65 0.2736952991113455 1101 66-67 0.28765417904112667 1101 68-69 0.1963075948720885 1101 70-71 0.42626118891177356 1101 72-73 0.520580316147786 1101 74-75 0.38972968511101413 1101 76-77 0.31631932698809706 1101 78-79 0.4100352449075011 1101 80-81 0.40673221454502695 1101 82-83 0.5441917651364179 1101 84-85 0.5458673545692925 1101 86-87 0.35511771538853054 1101 88-89 0.32221190246648135 1101 90-91 0.40093800808729085 1101 92-93 0.5286971868899712 1101 94-95 0.6064198439363011 1101 96-97 0.6346208831814231 1101 98-99 0.7762316663499007 1101 100-101 1.0818749275975286 1102 1 0.3689742457133285 1102 2 0.25624345121287107 1102 3 0.18340325933773372 1102 4 0.2932644006919176 1102 5 0.2398999064427656 1102 6 0.13411044554784723 1102 7 0.14255794617989892 1102 8 0.1342110235317051 1102 9 0.14848161830265383 1102 10-11 0.30223919636484453 1102 12-13 0.34901761174065626 1102 14-15 0.4356756835259077 1102 16-17 0.40106056274849067 1102 18-19 0.4605942116574866 1102 20-21 0.3619459900700406 1102 22-23 0.3278924867645543 1102 24-25 0.4630813811285961 1102 26-27 0.44185433853164824 1102 28-29 0.36841240569100364 1102 30-31 0.3812003119294829 1102 32-33 0.23929749387542643 1102 34-35 0.3173458338018236 1102 36-37 0.4092399422972477 1102 38-39 0.4140691202047009 1102 40-41 0.23390074576783348 1102 42-43 0.20345041345925097 1102 44-45 0.24727471147897973 1102 46-47 0.23403428604809307 1102 48-49 0.07906253699151478 1102 50-51 -0.024241610148806103 1102 52-53 0.1230952878017959 1102 54-55 0.2220393547389179 1102 56-57 0.17447716473035513 1102 58-59 0.24068390088336855 1102 60-61 0.16940682628238335 1102 62-63 0.1985734327922799 1102 64-65 0.0571590379637712 1102 66-67 0.14416707403849216 1102 68-69 0.215980685552573 1102 70-71 0.16367884994310344 1102 72-73 0.06799535513265909 1102 74-75 0.19898612848048458 1102 76-77 0.12170025149690034 1102 78-79 0.153838853627974 1102 80-81 0.027460678923905135 1102 82-83 0.1698951497441783 1102 84-85 0.33540644732057245 1102 86-87 0.168508011132662 1102 88-89 0.17165533697571078 1102 90-91 0.1565826340792036 1102 92-93 0.3628717171152509 1102 94-95 0.4843701623497765 1102 96-97 0.5061715759075156 1102 98-99 0.5766825757561307 1102 100-101 0.646530696254576 1103 1 0.42396482003912084 1103 2 0.38766072080210634 1103 3 0.40474240770327086 1103 4 0.3178050392259095 1103 5 0.38202477183477157 1103 6 0.30381967234357177 1103 7 0.4380071120712614 1103 8 0.39334804737070783 1103 9 0.366355965569106 1103 10-11 0.5122561989555905 1103 12-13 0.6040399039709428 1103 14-15 0.6857040876194773 1103 16-17 0.6782967248471721 1103 18-19 0.5668542994709824 1103 20-21 0.6083649564301226 1103 22-23 0.6771484739239071 1103 24-25 0.6599299718619847 1103 26-27 0.8361299419566848 1103 28-29 0.7826254403793769 1103 30-31 0.707121962476414 1103 32-33 0.777748165183759 1103 34-35 0.6585011727996957 1103 36-37 0.6031716319471485 1103 38-39 0.7210970653479194 1103 40-41 0.6956124013627161 1103 42-43 0.6768878519481945 1103 44-45 0.7746176380872924 1103 46-47 0.8211478969409214 1103 48-49 0.9396794141059388 1103 50-51 0.7568590036287759 1103 52-53 0.7627412731323275 1103 54-55 0.8891315429653659 1103 56-57 0.8492567713884753 1103 58-59 0.8089932543513854 1103 60-61 0.7460469259857376 1103 62-63 0.9062510842022888 1103 64-65 0.8151151740702147 1103 66-67 0.8971338068650994 1103 68-69 0.9675922441250506 1103 70-71 0.8281902521934086 1103 72-73 0.9409168108374715 1103 74-75 0.9830088756745781 1103 76-77 0.8805280693698485 1103 78-79 0.9192306332839593 1103 80-81 0.9184581011278325 1103 82-83 0.9828647827782362 1103 84-85 0.9749470385144079 1103 86-87 1.1088261519302556 1103 88-89 1.1393357141820708 1103 90-91 1.1046446277575512 1103 92-93 1.2445902195916396 1103 94-95 1.1399908341514404 1103 96-97 1.2609868172451364 1103 98-99 1.1403190820924891 1103 100-101 1.3063234061695468 1104 1 0.2808380359990146 1104 2 0.25578418428350247 1104 3 0.29343873932516473 1104 4 0.38629904300234585 1104 5 0.3987012744909748 1104 6 0.3490700084132996 1104 7 0.35547991633053044 1104 8 0.4700804424495715 1104 9 0.3416018693619378 1104 10-11 0.4166933168176712 1104 12-13 0.4222327464519111 1104 14-15 0.5480272769905881 1104 16-17 0.5035047037240403 1104 18-19 0.35065588428972916 1104 20-21 0.5100129266078568 1104 22-23 0.5545388504825652 1104 24-25 0.43287089705478365 1104 26-27 0.3935000740541028 1104 28-29 0.4812144340287219 1104 30-31 0.38930204428135795 1104 32-33 0.5246405844333637 1104 34-35 0.4722625898049486 1104 36-37 0.5893118874817773 1104 38-39 0.437697110011257 1104 40-41 0.5520162387122625 1104 42-43 0.5380306009426619 1104 44-45 0.6009086299588517 1104 46-47 0.5588371383793529 1104 48-49 0.6308698867053693 1104 50-51 0.6047433576913122 1104 52-53 0.6233996827574302 1104 54-55 0.7174709425013148 1104 56-57 0.6786386804007876 1104 58-59 0.6935117636078374 1104 60-61 0.6249305809064651 1104 62-63 0.6660969664905494 1104 64-65 0.5923457016084654 1104 66-67 0.7262820604458256 1104 68-69 0.7599839805315654 1104 70-71 0.5558359590394062 1104 72-73 0.7054562359648386 1104 74-75 0.6354862557367298 1104 76-77 0.7124070871850741 1104 78-79 0.7146714453588316 1104 80-81 0.6895790439037803 1104 82-83 0.8133064730374926 1104 84-85 0.6286986373695065 1104 86-87 0.5043500631154068 1104 88-89 0.6227750053680694 1104 90-91 0.73773517513645 1104 92-93 0.681313871748884 1104 94-95 0.5276624145962927 1104 96-97 0.8164259167388117 1104 98-99 0.7178799139982637 1104 100-101 0.9400526441633552 1105 1 0.17114771392906647 1105 2 0.220019785277497 1105 3 0.24729826379209285 1105 4 0.24344334733898165 1105 5 0.17093764256498645 1105 6 0.15701494073780964 1105 7 0.29610990199725506 1105 8 0.21848220202181778 1105 9 0.19763065653681622 1105 10-11 0.30588147004897337 1105 12-13 0.3136441595471098 1105 14-15 0.28483531474524426 1105 16-17 0.28806429707739056 1105 18-19 0.3382860122526239 1105 20-21 0.33936978090129344 1105 22-23 0.430710437989525 1105 24-25 0.3554543594127395 1105 26-27 0.29353336287669407 1105 28-29 0.2963039769564588 1105 30-31 0.34605814393390943 1105 32-33 0.5030272332831629 1105 34-35 0.14520391289547518 1105 36-37 0.2163784802046962 1105 38-39 0.43791962446940147 1105 40-41 0.3488889269884723 1105 42-43 0.3985370056838562 1105 44-45 0.32169091493379653 1105 46-47 0.5355228296984293 1105 48-49 0.5266107314545962 1105 50-51 0.420033858620954 1105 52-53 0.46712468080203706 1105 54-55 0.6357444093543236 1105 56-57 0.6764315584303446 1105 58-59 0.7215827458377788 1105 60-61 0.7128770351403375 1105 62-63 0.5195400390700868 1105 64-65 0.7008782181680715 1105 66-67 0.6547914279629126 1105 68-69 0.670404065211919 1105 70-71 0.6597624616539761 1105 72-73 0.645749917355225 1105 74-75 0.6927532787406463 1105 76-77 0.7550097124080573 1105 78-79 0.8159261253479357 1105 80-81 0.8653016210287809 1105 82-83 0.8845168715892768 1105 84-85 0.9159909532820834 1105 86-87 0.8453922836141601 1105 88-89 0.8430943566106173 1105 90-91 0.8094227605966076 1105 92-93 0.5863542659563912 1105 94-95 0.5271246811386945 1105 96-97 0.6867471055809027 1105 98-99 0.8581299877378363 1105 100-101 1.0173204069188877 1106 1 0.09148712468608622 1106 2 0.09014899761227468 1106 3 0.07290819195922538 1106 4 0.04797554731329967 1106 5 0.049262693984012174 1106 6 0.14433339134750867 1106 7 0.08285166364816376 1106 8 0.04672594174479627 1106 9 0.026259247501897676 1106 10-11 0.07234075721945032 1106 12-13 0.08952845815911559 1106 14-15 0.20139789219033588 1106 16-17 0.25107109325185206 1106 18-19 0.20408870069406504 1106 20-21 0.2052663079240915 1106 22-23 0.10482215971720166 1106 24-25 0.08899778564701677 1106 26-27 0.21764237119960939 1106 28-29 0.21458097397714226 1106 30-31 0.19428972760355379 1106 32-33 0.14847273816639017 1106 34-35 -0.03556559965041117 1106 36-37 0.07073941911509962 1106 38-39 0.06592065013640536 1106 40-41 0.0685417558938255 1106 42-43 0.037777179992978915 1106 44-45 0.06189439315691203 1106 46-47 0.08681952061088083 1106 48-49 0.244384753679185 1106 50-51 0.27939876814699005 1106 52-53 0.3049646025836168 1106 54-55 0.20861379871162455 1106 56-57 0.19974535826428763 1106 58-59 0.1434380453886206 1106 60-61 0.19368715831082284 1106 62-63 0.12014291238483565 1106 64-65 0.003157613838553175 1106 66-67 0.0029488452357213646 1106 68-69 0.0029327232885449916 1106 70-71 0.24660656388783053 1106 72-73 0.3575114868609006 1106 74-75 0.32795387171004364 1106 76-77 0.3331711455199162 1106 78-79 0.27764247144090604 1106 80-81 0.2479577186824038 1106 82-83 0.31675388387738224 1106 84-85 0.31910589672568435 1106 86-87 0.28094620710700013 1106 88-89 0.34590340889068827 1106 90-91 0.4429580013931904 1106 92-93 0.25497835266957125 1106 94-95 0.24840080444304036 1106 96-97 0.18203098198712553 1106 98-99 0.3162323048046076 1106 100-101 0.3543560303055422 1107 1 -0.09682762912575527 1107 2 0.08622744574729424 1107 3 0.14091959423815936 1107 4 0.045193368455954896 1107 5 0.012807032169291688 1107 6 0.1516041964221131 1107 7 -0.01844852290695087 1107 8 0.0751651479546851 1107 9 -0.02275278929239022 1107 10-11 0.07140964392996096 1107 12-13 0.054198633657705386 1107 14-15 0.21712518166992112 1107 16-17 -0.025066332515550016 1107 18-19 0.07558319332874675 1107 20-21 0.18473930872578137 1107 22-23 0.25443218923926736 1107 24-25 0.14376486721833004 1107 26-27 0.17963129187759108 1107 28-29 0.2390577837707184 1107 30-31 0.07243737020802854 1107 32-33 0.17347767750748488 1107 34-35 0.005113436266199756 1107 36-37 -0.04573594307158402 1107 38-39 0.032447352974244836 1107 40-41 0.1043515038734526 1107 42-43 -0.2790682528262849 1107 44-45 0.0467042570210765 1107 46-47 0.12423859140683646 1107 48-49 0.21076945046063145 1107 50-51 0.20529440366921392 1107 52-53 0.14738299896954032 1107 54-55 0.3081707303169523 1107 56-57 0.06273729763957903 1107 58-59 0.22007192242942608 1107 60-61 0.24518216387708236 1107 62-63 0.36329123889807846 1107 64-65 0.20994027903102364 1107 66-67 0.12402111919438141 1107 68-69 0.32829566987147274 1107 70-71 0.25768042413487535 1107 72-73 0.11854117800010755 1107 74-75 0.040772506704129796 1107 76-77 0.028619447716408786 1107 78-79 0.02058942481732373 1107 80-81 0.0014485030117761255 1107 82-83 0.11265888515021061 1107 84-85 0.002690044100422284 1107 86-87 -0.21323083217596306 1107 88-89 -0.04566143135962264 1107 90-91 0.11194307128486969 1107 92-93 -0.16476685331995 1107 94-95 0.019440022811309632 1107 96-97 0.14886805592146501 1107 98-99 0.1886605228803191 1107 100-101 0.3482502448173257 1108 1 -0.16858161755654066 1108 2 -0.08694690302393937 1108 3 -0.058042308418336575 1108 4 -0.05099505496248469 1108 5 0.00447738329858538 1108 6 -0.10226725267703785 1108 7 -0.1480697601494967 1108 8 -0.05187143875914302 1108 9 -0.07902835025182497 1108 10-11 -0.02706360687140119 1108 12-13 -0.13962298308449306 1108 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-1.595313706043953 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20269.0 3 4356.0 4 3714.0 5 2995.0 6 3033.0 7 3235.0 8 3837.0 9 4535.0 10 5334.0 11 6323.0 12 8138.0 13 11729.0 14 11829.0 15 10385.0 16 12215.0 17 15038.0 18 14183.0 19 10872.0 20 10755.0 21 11239.0 22 11748.0 23 12814.0 24 14422.0 25 17084.0 26 20123.0 27 23780.0 28 28484.0 29 34604.0 30 42046.0 31 52708.0 32 66766.0 33 87088.0 34 121382.0 35 182315.0 36 302954.0 37 497570.0 38 477722.0 39 91351.0 40 54.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.31595363583502 17.72690172924536 12.940797692863315 24.01634694205631 2 15.682619906072691 18.716796058736442 40.81906596870507 24.7815180664858 3 20.203709213000838 23.12578138558036 28.00578889396531 28.664720507453488 4 12.734891841736198 14.908770239832272 35.841148305024724 36.515189613406804 5 14.319606418672889 37.195037999474955 33.48743850291764 14.99791707893452 6 34.581605544977684 34.43396790220412 16.70682617139513 14.277600381423067 7 30.698229535716294 31.33995809462929 19.430970574602938 18.530841795051483 8 27.340429346736162 35.53636467068969 18.478927337064768 18.644278645509374 9 26.999985832658908 16.24595566684789 19.231014434749653 37.52304406574354 10-11 26.217071123076202 25.91560743052667 26.321985800218993 21.545335646178128 12-13 26.52135291775213 22.723379934758448 27.287344613034186 23.467922534455237 14-15 23.17356583671801 24.3976041626777 25.040170052299093 27.3886599483052 16-17 23.377736001847154 27.688240181297306 25.645878295527275 23.288145521328268 18-19 23.23969024755271 26.331348699945966 27.560778060109225 22.868182992392104 20-21 23.84359257733638 25.58533369426855 27.771706855385908 22.799366873009166 22-23 23.48548470997089 25.50870474693701 27.60386484123383 23.401945701858267 24-25 23.16346346275434 25.68587585430086 27.86118839691628 23.289472286028516 26-27 23.167664398942392 25.981413293578985 27.580396857281674 23.270525450196946 28-29 23.022644676288472 26.12741346673429 27.71698814493319 23.132953712044053 30-31 23.212847636204415 25.91386976423362 27.65780072687218 23.215481872689786 32-33 23.075361631659558 25.87849813758063 27.736977286828964 23.30916294393085 34-35 23.38765158556212 26.06501590311307 27.477476877681404 23.069855633643407 36-37 23.55036375584161 25.850314721354017 27.381347103018012 23.217974419786362 38-39 23.231386571580103 26.13241626834158 27.465896367653226 23.170300792425095 40-41 23.469780398206744 25.979191077492754 27.44675437215133 23.104274152149177 42-43 23.291775520647658 25.918571180440107 27.333882356970484 23.45577094194175 44-45 23.356058499694154 26.107012178168272 27.387933047878555 23.148996274259023 46-47 23.482642886760118 26.304096944286755 27.03247818682365 23.180781982129478 48-49 23.57202635909935 26.339322460271593 26.86339887514221 23.22525230548685 50-51 23.411537170276063 26.523731135310936 26.788442494694316 23.27628919971869 52-53 23.63336851905201 26.456538592875283 26.70231213551359 23.20778075255912 54-55 23.536859309789207 26.34599690330238 26.741836803012266 23.37530698389615 56-57 23.39390518158098 26.616315052792682 26.711889257588382 23.277890508037952 58-59 23.477993002777982 26.391270598544953 26.761816430794006 23.368919967883063 60-61 23.401802186510622 26.48595871407109 26.88574030765946 23.22649879175883 62-63 23.324959222915766 26.487512893653985 27.001902411663735 23.18562547176651 64-65 23.271586442866166 26.461408693946165 26.92950969289825 23.337495170289422 66-67 23.26139717998656 26.628703863897858 26.67969502261357 23.430203933502014 68-69 23.278102960365192 26.428187242887148 26.954813980088062 23.3388958166596 70-71 23.368673087884723 26.308171763172123 26.815127588515995 23.508027560427156 72-73 23.274755156923543 26.640448056212986 26.6271252298966 23.457671556966872 74-75 23.20775412899628 26.825023362443424 26.53980246306109 23.427420045499208 76-77 23.4751926534249 26.6740168597742 26.454845578107072 23.39594490869383 78-79 23.460761794993328 26.689003364961916 26.520632939349504 23.329601900695256 80-81 23.41356119115559 26.734139949193743 26.479797273206295 23.37250158644437 82-83 23.521934408632525 26.85219310722794 26.452275581876663 23.17359690226287 84-85 23.516772862880437 26.75357206962842 26.27758527003243 23.45206979745872 86-87 23.413549023240833 26.61095142016937 26.59119981440608 23.384299742183714 88-89 23.46074359482024 26.735799821587076 26.388628734269297 23.414827849323387 90-91 23.35929931024907 26.9009538313241 26.463579023632395 23.27616783479444 92-93 23.347681633708294 26.751314741727256 26.448151552226236 23.452852072338217 94-95 23.422664187119924 26.614535686634046 26.58146171816041 23.381338408085618 96-97 23.562658905913466 26.47649915791262 26.48403725197113 23.47680468420278 98-99 23.29265960805589 26.56770974613014 26.366557126276795 23.773073519537174 100-101 23.39801546394045 26.652127159023493 26.217164920532284 23.732692456503777 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 103.0 1 80.5 2 134.0 3 240.5 4 214.0 5 207.0 6 414.5 7 826.5 8 1297.0 9 1527.5 10 1442.0 11 1399.5 12 1573.5 13 1784.5 14 2025.5 15 2261.5 16 2458.0 17 2655.5 18 2821.5 19 3012.0 20 3213.0 21 3502.0 22 4028.0 23 4777.0 24 5796.0 25 7177.5 26 9218.5 27 11930.0 28 14364.0 29 17050.0 30 20520.0 31 24522.5 32 29086.0 33 32948.5 34 36219.0 35 39892.5 36 45238.5 37 50264.0 38 53270.5 39 56225.0 40 59675.5 41 63307.5 42 65749.5 43 67802.0 44 70753.0 45 74182.5 46 77208.0 47 79795.5 48 82524.5 49 84094.0 50 83936.0 51 84390.5 52 85138.5 53 86329.5 54 88185.0 55 85397.5 56 78877.5 57 72253.0 58 67328.5 59 61304.5 60 52158.0 61 43721.5 62 36471.0 63 29181.5 64 22171.5 65 16507.0 66 11873.5 67 8976.0 68 6698.5 69 4736.5 70 3480.0 71 2341.0 72 1761.0 73 1445.0 74 1112.0 75 780.0 76 541.5 77 324.5 78 199.0 79 121.5 80 75.0 81 47.0 82 37.0 83 32.5 84 31.5 85 28.5 86 22.0 87 22.0 88 22.0 89 19.5 90 23.0 91 20.0 92 15.5 93 15.5 94 16.0 95 21.0 96 22.0 97 16.5 98 13.5 99 15.5 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04391267221447799 2 0.011553636540301166 3 0.009782964273588343 4 0.005179216380135005 5 0.00805655881354334 6 0.005312016800138467 7 0.005754684866816672 8 0.008941894946899752 9 0.013855510487027834 10-11 0.002235473736724938 12-13 0.008521360283555457 14-15 0.008609893896891098 16-17 0.018525658590482903 18-19 0.011398702716963792 20-21 0.0036520115500951957 22-23 0.009030428560235393 24-25 0.020229930647193993 26-27 0.006927755243513917 28-29 0.016577919097098798 30-31 0.013722710067024373 32-33 0.012106971623648922 34-35 0.26608777488026936 36-37 0.4697372189555778 38-39 0.4670812105555086 40-41 0.48919248048608494 42-43 0.47788231138245674 44-45 0.49375196157287043 46-47 0.5004362493797113 48-49 0.5071205371865523 50-51 0.5168371012501388 52-53 0.5060360004231906 54-55 0.4935970277495331 56-57 0.4885948785960693 58-59 0.4866692725060192 60-61 0.4836812630559413 62-63 0.4794095162124966 64-65 0.49682850463628403 66-67 0.46922815067889784 68-69 0.49720477249296047 70-71 0.478324979449135 72-73 0.4872668743960347 74-75 0.5001042483297027 76-77 0.48762100884937726 78-79 0.4873554080093704 80-81 0.4903655508627822 82-83 0.4898343491827683 84-85 0.489922882796104 86-87 0.4918263554828202 88-89 0.5061466674398603 90-91 0.5305819447204971 92-93 0.5190061747768621 94-95 0.4885948785960693 96-97 0.46258812967872476 98-99 0.4532478334718147 100-101 0.46227826203205 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2259029.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.24208556316439 #Duplication Level Percentage of deduplicated Percentage of total 1 72.46018034509032 37.85470941510531 2 14.297021005409102 14.938123893258817 3 5.377839904194087 8.428487172597217 4 2.608375089629428 5.4506781845298855 5 1.4513099382172194 3.79097289855074 6 0.9088706043812644 2.8488777527958566 7 0.5990758664747184 2.190788087263936 8 0.404706993591838 1.6914189909788653 9 0.3026952796760646 1.4232089430362647 >10 1.461227223941281 13.51498873285932 >50 0.08851471437667961 3.168562493777923 >100 0.03780179348241422 3.382576311440889 >500 0.0018709754922444236 0.6783854414989186 >1k 5.10266043339343E-4 0.6382216823060647 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4975 0.22022736317240726 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3526 0.15608476031073526 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2392 0.10588620154942675 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.426680666782055E-5 2 0.0 0.0 0.0 0.0 8.85336133356411E-5 3 0.0 0.0 0.0 0.0 8.85336133356411E-5 4 0.0 0.0 0.0 0.0 8.85336133356411E-5 5 0.0 0.0 0.0 0.0 8.85336133356411E-5 6 0.0 0.0 0.0 0.0 8.85336133356411E-5 7 0.0 0.0 0.0 0.0 8.85336133356411E-5 8 0.0 0.0 0.0 0.0 8.85336133356411E-5 9 0.0 0.0 0.0 0.0 8.85336133356411E-5 10-11 0.0 0.0 0.0 0.0 1.3280042000346167E-4 12-13 0.0 0.0 0.0 0.0 1.3280042000346167E-4 14-15 0.0 0.0 0.0 0.0 1.3280042000346167E-4 16-17 0.0 0.0 0.0 1.1066701666955139E-4 1.3280042000346167E-4 18-19 0.0 0.0 0.0 1.770672266712822E-4 1.770672266712822E-4 20-21 0.0 0.0 0.0 2.877342433408336E-4 2.2133403333910278E-4 22-23 0.0 0.0 0.0 5.754684866816672E-4 2.6560084000692335E-4 24-25 0.0 0.0 0.0 9.738697466920522E-4 2.6560084000692335E-4 26-27 0.0 0.0 0.0 0.0010624033600276934 2.6560084000692335E-4 28-29 0.0 0.0 0.0 0.0013058707967007065 2.6560084000692335E-4 30-31 0.0 0.0 0.0 0.002810942223406605 2.6560084000692335E-4 32-33 0.0 0.0 0.0 0.005887485286820133 2.6560084000692335E-4 34-35 0.0 0.0 0.0 0.009229629190240585 2.6560084000692335E-4 36-37 0.0 0.0 0.0 0.01509498107372681 2.6560084000692335E-4 38-39 0.0 0.0 0.0 0.02543128043066291 3.098676466747439E-4 40-41 0.0 0.0 0.0 0.037848119700986574 3.098676466747439E-4 42-43 0.0 0.0 0.0 0.05088469426465973 3.098676466747439E-4 44-45 0.0 0.0 0.0 0.06533780664170313 3.541344533425644E-4 46-47 0.0 0.0 0.0 0.08435040010553208 4.2053466334429526E-4 48-49 0.0 0.0 0.0 0.10581980133942503 4.869348733460261E-4 50-51 0.0 0.0 0.0 0.12985667736005158 4.869348733460261E-4 52-53 0.0 0.0 0.0 0.15411488741401724 6.861355033512186E-4 54-55 0.0 0.0 0.0 0.1785280312913203 7.082689066851288E-4 56-57 0.0 0.0 0.0 0.21040013209215108 7.525357133529494E-4 58-59 0.0 0.0 0.0 0.24756211628978647 7.9680252002077E-4 60-61 0.0 0.0 0.0 0.2872915752741554 7.9680252002077E-4 62-63 0.0 0.0 0.0 0.328570372491898 9.738697466920522E-4 64-65 0.0 0.0 0.0 0.37051317180965804 9.738697466920522E-4 66-67 0.0 0.0 0.0 0.4180114553642295 9.960031500259624E-4 68-69 0.0 0.0 0.0 0.47805937860912806 0.0010181365533598727 70-71 0.0 0.0 0.0 0.5461195938609023 0.0010181365533598727 72-73 0.0 0.0 0.0 0.6167472838994099 0.0010624033600276934 74-75 0.0 0.0 0.0 0.6873528405345837 0.0011066701666955139 76-77 0.0 0.0 0.0 0.7662584234199739 0.0011509369733633344 78-79 0.0 0.0 0.0 0.8601040535557534 0.0011730703766972446 80-81 0.0 0.0 0.0 0.9680929284219015 0.001195203780031155 82-83 0.0 0.0 0.0 1.0803756835348284 0.001195203780031155 84-85 0.0 0.0 0.0 1.1977933882212224 0.0012394705866989755 86-87 0.0 0.0 0.0 1.321983914327793 0.0012837373933667962 88-89 0.0 0.0 0.0 1.4617120895747686 0.001350137603368527 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2985 0.0 48.84382 1 GTATCAA 5420 0.0 43.14504 1 ATCAACG 6695 0.0 34.50042 3 TCAACGC 6695 0.0 34.42896 4 CAACGCA 6810 0.0 33.91706 5 TATCAAC 7145 0.0 32.393074 2 AACGCAG 7205 0.0 32.121185 6 GTACATG 8815 0.0 27.011513 1 ACGCAGA 8675 0.0 26.405972 7 CGCAGAG 8740 0.0 26.21075 8 TACATGG 8800 0.0 25.8169 2 ACATGGG 8950 0.0 24.432848 3 TACCTGG 1860 0.0 23.411022 2 GCAGAGT 10115 0.0 22.272911 9 GTACCTG 2110 0.0 22.210379 1 GTGGTAT 1890 0.0 21.539711 1 TGGTATC 1700 0.0 21.43815 2 GTGTAGG 1120 0.0 20.710056 1 CATGGGG 5515 0.0 20.425701 4 GAGTACA 6795 0.0 19.715157 1 >>END_MODULE