FastQCFastQC Report
Thu 26 May 2016
SRR936302_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936302_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259029
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52070.23049726231934164No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39860.17644749137793275No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25670.11363289271629537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33200.053.8908461
GTATCAA61300.040.7534261
ATCAACG75550.032.2720683
TCAACGC76050.032.247014
CAACGCA77550.031.6232785
TATCAAC78750.031.2106132
AACGCAG80450.030.660236
GTACATG89150.026.2608661
TACATGG88700.026.158232
ACGCAGA96250.025.528617
TGGTATC17000.025.3989412
ACATGGG87800.024.7979813
CGCAGAG97150.024.755028
GTACCTG20400.024.0254941
GTGGTAT19900.023.9117971
TACCTGG20400.023.2591022
GCAGAGT109150.021.4684839
GAGTACA65450.020.3569931
CATGGGG54650.020.0502574
TACGCTC1456.205589E-419.628244
Produced by FastQC (version 0.11.5)