##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936302_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2259029 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.464455303583975 34.0 31.0 34.0 31.0 34.0 2 32.60322775847499 34.0 31.0 34.0 31.0 34.0 3 32.687671118874526 34.0 31.0 34.0 31.0 34.0 4 36.17817920885478 37.0 35.0 37.0 35.0 37.0 5 36.139566601402635 37.0 35.0 37.0 35.0 37.0 6 36.173114643503915 37.0 37.0 37.0 35.0 37.0 7 36.12393820530856 37.0 36.0 37.0 35.0 37.0 8 36.103259851909826 37.0 36.0 37.0 35.0 37.0 9 37.869080919279924 39.0 38.0 39.0 35.0 39.0 10-11 37.80377985408775 39.0 38.0 39.0 35.0 39.0 12-13 37.744656000432045 39.0 38.0 39.0 35.0 39.0 14-15 39.093469583613135 40.0 38.0 41.0 36.0 41.0 16-17 39.01243122598249 40.0 38.0 41.0 35.5 41.0 18-19 39.03468370702634 40.0 38.5 41.0 35.5 41.0 20-21 38.98757984071918 40.0 38.5 41.0 35.0 41.0 22-23 38.87211518754296 40.0 38.0 41.0 35.0 41.0 24-25 39.03017048475252 40.0 39.0 41.0 35.0 41.0 26-27 38.95280582940724 40.0 38.0 41.0 35.0 41.0 28-29 38.86264186958202 40.0 38.0 41.0 35.0 41.0 30-31 38.74598422596611 40.0 38.0 41.0 35.0 41.0 32-33 38.59419865791895 40.0 38.0 41.0 34.0 41.0 34-35 38.46145622743222 40.0 38.0 41.0 34.0 41.0 36-37 38.317198672527 40.0 38.0 41.0 34.0 41.0 38-39 38.179421999451975 40.0 38.0 41.0 33.5 41.0 40-41 38.032706087438456 40.0 38.0 41.0 33.0 41.0 42-43 38.05757606476057 40.0 38.0 41.0 33.0 41.0 44-45 37.92601622201397 40.0 38.0 41.0 33.0 41.0 46-47 37.72259320265477 40.0 37.0 41.0 33.0 41.0 48-49 37.49447063317912 40.0 37.0 41.0 32.5 41.0 50-51 37.28688454198684 40.0 37.0 41.0 32.0 41.0 52-53 37.06838801095515 40.0 36.0 41.0 31.5 41.0 54-55 36.865560380145624 39.5 36.0 41.0 31.0 41.0 56-57 36.60660531582374 39.0 35.0 41.0 31.0 41.0 58-59 36.33218232258196 39.0 35.0 41.0 30.5 41.0 60-61 36.04546665846255 38.5 35.0 40.0 30.0 41.0 62-63 35.73819105465224 38.0 35.0 40.0 29.5 41.0 64-65 35.35867224369409 37.0 35.0 40.0 29.0 41.0 66-67 35.007502559728096 37.0 34.0 39.5 29.0 41.0 68-69 34.64257851492832 36.0 34.0 39.0 28.5 41.0 70-71 34.2646181168989 36.0 34.0 39.0 28.0 40.5 72-73 33.858225591614804 35.0 34.0 38.0 27.5 40.0 74-75 33.4282065436079 35.0 33.0 37.0 26.5 39.5 76-77 32.71485669285343 35.0 32.5 36.0 26.0 39.0 78-79 32.77780785461364 35.0 33.0 36.0 26.0 39.0 80-81 32.60919625201801 35.0 33.0 36.0 26.0 37.5 82-83 32.32289846655355 35.0 33.0 35.5 26.0 37.0 84-85 32.030447816296295 35.0 33.0 35.0 25.5 37.0 86-87 31.62415201398477 35.0 32.0 35.0 25.0 36.0 88-89 31.12801119419007 34.5 32.0 35.0 23.5 36.0 90-91 30.904628271704347 34.0 32.0 35.0 21.5 36.0 92-93 30.60921440140875 34.0 31.0 35.0 20.0 35.0 94-95 30.437202665392963 34.0 31.0 35.0 19.0 35.0 96-97 30.1700699725413 34.0 31.0 35.0 16.5 35.0 98-99 29.698261288367704 34.0 31.0 35.0 2.0 35.0 100-101 26.260806966178833 30.5 24.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.055827363892952064 1101 2 0.05566795519377621 1101 3 0.07652480218332158 1101 4 0.08449372475595851 1101 5 0.06174008381626095 1101 6 0.11031424027179071 1101 7 0.08698825892584239 1101 8 0.11521931532390539 1101 9 0.22776661695861122 1101 10-11 0.1410541444876472 1101 12-13 0.2788871946150593 1101 14-15 0.43297275553768344 1101 16-17 0.3545018598481917 1101 18-19 0.32991454184838176 1101 20-21 0.23807250684994585 1101 22-23 0.28444852848978286 1101 24-25 0.22922037019977637 1101 26-27 0.23566918328945263 1101 28-29 0.2854009823339254 1101 30-31 0.31816134339336344 1101 32-33 0.3232632839467513 1101 34-35 0.3123688527168582 1101 36-37 0.23875082458813068 1101 38-39 0.3366546628142828 1101 40-41 0.29844148288783146 1101 42-43 0.27943872665273517 1101 44-45 0.29323334793421196 1101 46-47 0.22780015094467387 1101 48-49 0.2520186685846326 1101 50-51 0.31929073008668496 1101 52-53 0.2925863981110908 1101 54-55 0.33911418642657765 1101 56-57 0.30822494065411377 1101 58-59 0.27675516560698554 1101 60-61 0.3146515547323929 1101 62-63 0.3864094232893862 1101 64-65 0.442193515490402 1101 66-67 0.4408161615334265 1101 68-69 0.48793242336702036 1101 70-71 0.49541038248722913 1101 72-73 0.6299503565993589 1101 74-75 0.523342236920449 1101 76-77 0.3703303963707043 1101 78-79 0.39122543615002314 1101 80-81 0.4118713382786723 1101 82-83 0.3029748676073538 1101 84-85 0.42763712866767634 1101 86-87 0.3029041064710576 1101 88-89 0.5086976019761842 1101 90-91 0.6795436061748248 1101 92-93 0.8880835731462291 1101 94-95 0.716497154405598 1101 96-97 0.5865342917025522 1101 98-99 0.6121797955366155 1101 100-101 0.9278488021872953 1102 1 0.011186764910178226 1102 2 0.06731748048981956 1102 3 0.03534318830340055 1102 4 0.03307348400254284 1102 5 -9.88764578870871E-4 1102 6 0.10254023383642163 1102 7 0.029605701315666977 1102 8 0.09561900191709327 1102 9 -0.028593910299832714 1102 10-11 0.07300274824790876 1102 12-13 0.13019419186604608 1102 14-15 0.09533459485165707 1102 16-17 0.19994267334326565 1102 18-19 0.20446574866074485 1102 20-21 0.1300240629932219 1102 22-23 0.1680130199934311 1102 24-25 0.19873701554941192 1102 26-27 0.1031430583128028 1102 28-29 0.12334276877339789 1102 30-31 0.13147473265680532 1102 32-33 0.18283101791019618 1102 34-35 0.14528023270816703 1102 36-37 0.07585412392776192 1102 38-39 3.01072623628329E-4 1102 40-41 0.004703889864543953 1102 42-43 -0.04937247980864612 1102 44-45 0.02416157302554467 1102 46-47 0.08421162229468138 1102 48-49 -0.0152460621033228 1102 50-51 -0.16598658229504082 1102 52-53 -0.07885267698782883 1102 54-55 -0.1492751286308831 1102 56-57 -0.007791530752676579 1102 58-59 -0.22910830835681395 1102 60-61 -0.192913797710915 1102 62-63 0.04036935862600188 1102 64-65 -0.11833252999900878 1102 66-67 -0.16258305329785827 1102 68-69 -0.08613006598736916 1102 70-71 -0.05664614325992545 1102 72-73 -0.23362425662298136 1102 74-75 -0.0687765004919143 1102 76-77 -0.18814304611821342 1102 78-79 -0.1531106338152597 1102 80-81 -0.13145733421223582 1102 82-83 -0.009298735714189377 1102 84-85 -0.005431522495882746 1102 86-87 -0.14252958414191141 1102 88-89 -3.745239150738655E-4 1102 90-91 0.13314331981431593 1102 92-93 0.061715437823853136 1102 94-95 -0.05749831275739936 1102 96-97 -0.09486925673149571 1102 98-99 0.04962243827039714 1102 100-101 0.6608510505405185 1103 1 0.03874322053775359 1103 2 0.06781878782560113 1103 3 0.1926027015569005 1103 4 0.0843862265274069 1103 5 0.13886526827918289 1103 6 0.1598103612512034 1103 7 0.1477535251883566 1103 8 0.18169561423559344 1103 9 0.12905952288302558 1103 10-11 0.2730543988496592 1103 12-13 0.2880331237126583 1103 14-15 0.3387914378213779 1103 16-17 0.302868643804004 1103 18-19 0.2264893858497814 1103 20-21 0.3119505382495191 1103 22-23 0.3179902468868292 1103 24-25 0.3184676201629273 1103 26-27 0.3398173027265372 1103 28-29 0.357363223707317 1103 30-31 0.37417650013890835 1103 32-33 0.30359413624115206 1103 34-35 0.3703613712438951 1103 36-37 0.44351122971009005 1103 38-39 0.46168620572172614 1103 40-41 0.3306213047133113 1103 42-43 0.3970349754187765 1103 44-45 0.4273462200648126 1103 46-47 0.42949963232880606 1103 48-49 0.3283444296014366 1103 50-51 0.3658177802747389 1103 52-53 0.3390334554858754 1103 54-55 0.48388326206293186 1103 56-57 0.3618872488479994 1103 58-59 0.41618087272327386 1103 60-61 0.37542969050113584 1103 62-63 0.39745827290693825 1103 64-65 0.3970672227358847 1103 66-67 0.23398755105653635 1103 68-69 0.2787469268463312 1103 70-71 0.14479481633998859 1103 72-73 0.1842071855126477 1103 74-75 0.2812572355981757 1103 76-77 0.1512580909424912 1103 78-79 0.12596220175542783 1103 80-81 0.3278952866633702 1103 82-83 0.38766429361727717 1103 84-85 0.3545151176735857 1103 86-87 0.3138954218254355 1103 88-89 0.5269990492152488 1103 90-91 0.5901689228656153 1103 92-93 0.6978571303988765 1103 94-95 0.6529245693810601 1103 96-97 0.6010904602840021 1103 98-99 0.3002878166204006 1103 100-101 0.7667021443223625 1104 1 0.060645285219806055 1104 2 0.06433572851336322 1104 3 0.04785261434662402 1104 4 0.011530979047265077 1104 5 0.056133243935235555 1104 6 0.015879101925058592 1104 7 9.505299227683395E-4 1104 8 0.04867500536433056 1104 9 -0.06366886709117381 1104 10-11 0.0459409544547853 1104 12-13 0.09623680118885858 1104 14-15 0.14639084659163615 1104 16-17 0.1333235919716671 1104 18-19 0.18883960189287308 1104 20-21 0.20598360371634072 1104 22-23 0.17178045179813495 1104 24-25 0.2676276769311059 1104 26-27 0.33440746793711185 1104 28-29 0.3077031126911862 1104 30-31 0.1773075072485497 1104 32-33 0.05135926643381339 1104 34-35 0.021630822055108467 1104 36-37 0.0864988665280535 1104 38-39 0.030176560532545693 1104 40-41 0.2511615511011982 1104 42-43 0.32114646445460693 1104 44-45 0.28112341033209987 1104 46-47 0.2611413457468288 1104 48-49 0.1708170027595486 1104 50-51 0.27804069607071824 1104 52-53 0.2799512287216359 1104 54-55 0.14296940538360303 1104 56-57 0.263048203748248 1104 58-59 0.3220891318893493 1104 60-61 0.19753872451290277 1104 62-63 0.3072296919701998 1104 64-65 0.25663702428566637 1104 66-67 0.27028150102289317 1104 68-69 0.38748099363797195 1104 70-71 0.456099384796282 1104 72-73 0.3501747914555011 1104 74-75 0.3188263774149789 1104 76-77 0.3670081698817853 1104 78-79 0.34166265476234514 1104 80-81 0.43239594518003344 1104 82-83 0.3475914748594491 1104 84-85 0.3528740562040795 1104 86-87 0.30550632824309076 1104 88-89 0.37216832959544277 1104 90-91 0.42721074049809715 1104 92-93 0.36687952872287966 1104 94-95 0.3779401018113653 1104 96-97 0.25733150511421954 1104 98-99 0.1363176544554996 1104 100-101 0.4104738222189219 1105 1 0.03619272497245163 1105 2 0.0604206991164844 1105 3 0.09444094587220064 1105 4 0.08767851284731876 1105 5 -0.0054904213314017625 1105 6 0.0303631558015951 1105 7 0.035902615692492645 1105 8 0.07281705840593133 1105 9 0.06930337007790399 1105 10-11 0.1282695370435718 1105 12-13 0.07799285372347953 1105 14-15 0.11251151845572593 1105 16-17 0.11541547760324278 1105 18-19 0.10812846029121914 1105 20-21 0.0451474676510486 1105 22-23 8.235006302257375E-4 1105 24-25 0.1258799595595761 1105 26-27 0.18184206117266655 1105 28-29 0.0749884457254808 1105 30-31 0.1854393564296899 1105 32-33 0.14429197636543023 1105 34-35 0.04931249232944168 1105 36-37 0.16659064284601754 1105 38-39 0.19556405373578656 1105 40-41 0.4035541528311839 1105 42-43 0.40850182416708236 1105 44-45 0.27601076076798137 1105 46-47 0.2515946627082499 1105 48-49 0.2641908543776239 1105 50-51 0.22447290231158235 1105 52-53 0.15939079855371574 1105 54-55 0.22900145783152226 1105 56-57 0.2717929394967129 1105 58-59 0.1730799514304806 1105 60-61 0.18221891622636122 1105 62-63 0.21814498616988232 1105 64-65 0.2013104357279758 1105 66-67 0.15420234006142408 1105 68-69 0.05607545143773507 1105 70-71 0.05541304136495029 1105 72-73 0.0357664561039428 1105 74-75 0.04201792031067697 1105 76-77 0.020833628080168864 1105 78-79 0.009297202387742232 1105 80-81 0.21709241614077968 1105 82-83 0.26277400503613535 1105 84-85 0.24110512633725278 1105 86-87 0.24507498237576186 1105 88-89 0.4288472029490258 1105 90-91 0.5174503069376897 1105 92-93 0.6057547481746717 1105 94-95 0.32201794252823746 1105 96-97 0.157394360442364 1105 98-99 0.2932131996430165 1105 100-101 0.5739106328246031 1106 1 -0.04961101265215717 1106 2 -0.06777049100093535 1106 3 -0.04303551534148653 1106 4 -0.04908115727501894 1106 5 -0.06563936916348467 1106 6 0.016288725300029228 1106 7 -0.022692455679099055 1106 8 -0.019990881378191716 1106 9 0.02388142659270187 1106 10-11 0.027223474284333804 1106 12-13 0.011288093868841997 1106 14-15 0.14602726796143628 1106 16-17 0.008859168123144912 1106 18-19 0.044773172498459246 1106 20-21 0.07683415622997103 1106 22-23 0.11820290335556649 1106 24-25 0.06782530796979103 1106 26-27 -0.052162643871184855 1106 28-29 0.1138419146325731 1106 30-31 0.13925811184518722 1106 32-33 0.0868580161150021 1106 34-35 0.10900264863964537 1106 36-37 0.0460385916024606 1106 38-39 0.13120525841513597 1106 40-41 0.052050203018318086 1106 42-43 0.09980296506333275 1106 44-45 0.0253633876151369 1106 46-47 0.030223570237225772 1106 48-49 -0.009851268517905964 1106 50-51 0.06847089620099922 1106 52-53 -0.050946007334736976 1106 54-55 0.0010937732883391504 1106 56-57 -0.02046992249409385 1106 58-59 0.005700186917835026 1106 60-61 -0.06414579974347134 1106 62-63 -0.056104259955823466 1106 64-65 -0.01937755709415967 1106 66-67 -0.028649258405323508 1106 68-69 0.0708763960643779 1106 70-71 -0.023834432002985295 1106 72-73 -0.0032970755551531283 1106 74-75 0.004222453143782445 1106 76-77 -0.06561228200483527 1106 78-79 -0.23918329094541946 1106 80-81 -0.12696097642919568 1106 82-83 -0.1358427916629168 1106 84-85 0.07234627385421533 1106 86-87 0.04846031660391503 1106 88-89 0.06360664152748896 1106 90-91 0.275755586680674 1106 92-93 0.3052818017246217 1106 94-95 0.2288427886329636 1106 96-97 0.17277936544407524 1106 98-99 0.1357970574171965 1106 100-101 0.5645136598989566 1107 1 -0.01964630994350358 1107 2 0.0574911990896112 1107 3 0.012671228378899002 1107 4 0.02280525307783421 1107 5 -0.008833622050147483 1107 6 0.017615223345188724 1107 7 0.01712290795139637 1107 8 0.03219088257449698 1107 9 0.008336750252524894 1107 10-11 0.07980608476409401 1107 12-13 0.0783211266622672 1107 14-15 0.06727992285727424 1107 16-17 0.09348694127820778 1107 18-19 -0.008649838371887597 1107 20-21 0.0069363056032401005 1107 22-23 0.046894015961150615 1107 24-25 0.07302657662054912 1107 26-27 0.14281309636506734 1107 28-29 0.10190010766962132 1107 30-31 0.0014697303152857444 1107 32-33 0.040320161764775264 1107 34-35 0.06899351085862548 1107 36-37 -0.026392571080428695 1107 38-39 -0.05661627369247668 1107 40-41 -0.108951894301903 1107 42-43 -0.23863356250389245 1107 44-45 -0.30009240908158574 1107 46-47 -0.28396008016380847 1107 48-49 -0.31837794855879054 1107 50-51 -0.1885011737248803 1107 52-53 -0.15287643481570967 1107 54-55 -0.04610614274286462 1107 56-57 -0.14288009297735016 1107 58-59 -0.09042675224848296 1107 60-61 -0.05788922187095835 1107 62-63 -0.13667678658077165 1107 64-65 -0.06650077085744499 1107 66-67 -0.21619224017155858 1107 68-69 -0.13961457444015224 1107 70-71 0.052399073447382705 1107 72-73 -0.09699115176539408 1107 74-75 -0.08583527065586338 1107 76-77 -0.14814534709395133 1107 78-79 -0.2792940651681306 1107 80-81 -0.08493644500168784 1107 82-83 -0.04348304995246366 1107 84-85 -0.25815050037610376 1107 86-87 -0.12423543328305087 1107 88-89 -0.030790103446729944 1107 90-91 -0.32845002479075447 1107 92-93 -0.0027250601600066204 1107 94-95 -0.037323342305171536 1107 96-97 -0.34359573761717854 1107 98-99 -0.17927282476052397 1107 100-101 0.12877828699770433 1108 1 -0.14204415821733107 1108 2 -0.075352100715115 1108 3 -0.0030365568906205453 1108 4 -0.06619294153547628 1108 5 -0.05072872539932405 1108 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-0.5647913747393005 2316 74-75 -0.6200301469011222 2316 76-77 -0.4446684531881502 2316 78-79 -0.5645164478640083 2316 80-81 -0.6276686250362289 2316 82-83 -0.7334957294823568 2316 84-85 -0.4777869325400914 2316 86-87 -0.6904574718051748 2316 88-89 -0.5585618106752257 2316 90-91 -0.7030966132476308 2316 92-93 -0.7563768914599684 2316 94-95 -0.7278163870393115 2316 96-97 -0.5417630123945223 2316 98-99 -0.8309359969022978 2316 100-101 -0.8455269254398523 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 13.0 5 35.0 6 52.0 7 151.0 8 360.0 9 696.0 10 1195.0 11 1888.0 12 2793.0 13 4209.0 14 5348.0 15 7215.0 16 9138.0 17 12293.0 18 11493.0 19 7965.0 20 7248.0 21 7763.0 22 8316.0 23 9483.0 24 11234.0 25 13581.0 26 16707.0 27 20934.0 28 26256.0 29 32544.0 30 40979.0 31 51051.0 32 63420.0 33 82319.0 34 114248.0 35 170733.0 36 294338.0 37 538339.0 38 577400.0 39 107235.0 40 50.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.485062722097304 18.152834012589693 13.459085272047277 23.903017993265728 2 15.921084381357604 19.083243492140095 40.18099587579114 24.814676250711166 3 20.1520653342653 23.162650855743774 28.430090981567744 28.255192828423187 4 13.003772859932297 15.276829115518217 35.71224627926423 36.007151745285256 5 14.247094658811374 37.18513573752262 33.59368117894901 14.974088424716992 6 34.60300863778198 34.669364580977046 16.462426998502455 14.265199782738513 7 30.525460275189033 31.408760135438722 19.433969196499913 18.631810392872335 8 27.266936369564093 35.71972736959109 18.31769313275748 18.695643128087333 9 26.741843508870407 16.10568080356649 19.25849557486867 37.893980112694436 10-11 26.132957595347715 25.913393456723067 26.328064236675974 21.625584711253236 12-13 26.37882894298542 22.75718201748319 27.390923749268214 23.47306529026318 14-15 23.00005887485287 24.45183306633071 25.11514903084467 27.432959027971755 16-17 23.285570670782914 27.739476949487322 25.704410369643078 23.27054201008669 18-19 23.11597150811256 26.389501861197886 27.628861780880193 22.865664849809363 20-21 23.68724513374779 25.562764162285728 27.915169710158526 22.834820993807956 22-23 23.32191101659652 25.501875142760515 27.75888568003584 23.417328160607127 24-25 23.05416080576635 25.71411496624011 27.99641871784344 23.235305510150102 26-27 23.076543394599362 25.948290230053605 27.760462882809385 23.21470349253765 28-29 22.92284754814337 26.086418572613685 27.918748356560606 23.071985522682343 30-31 23.136433699483224 25.899999203187956 27.745349495042497 23.21821760228632 32-33 22.950101594075228 25.840486234998828 27.84454557125087 23.36486659967508 34-35 23.259017689538755 26.069830749620237 27.648035704444084 23.023115856396917 36-37 23.452724679093105 25.827463366426485 27.570890571274585 23.148921383205824 38-39 23.177843017300376 26.062227703443874 27.631643017831582 23.128286261424172 40-41 23.349247348955185 25.926521072237236 27.581977100638994 23.142254478168585 42-43 23.249659076597258 25.92011690446641 27.42748419224339 23.402739826692937 44-45 23.272039903627473 26.085192518180524 27.44153514310347 23.201232435088542 46-47 23.401200017266437 26.322592356060465 27.126389200951422 23.149818425721676 48-49 23.455973259102255 26.352933363586555 26.974238642589526 23.21685473472166 50-51 23.371863515456894 26.520726989078874 26.84814610436108 23.25926339110315 52-53 23.554367146186895 26.407494062257204 26.789568166172685 23.248570625383213 54-55 23.440959744455334 26.269843799385395 26.882610054457288 23.40658640170198 56-57 23.314304635790904 26.59050760826957 26.845780341498386 23.249407414441137 58-59 23.39454993480524 26.38113625915532 26.848245496182937 23.376068309856503 60-61 23.340300278818 26.471663020880253 26.95878110695416 23.229255593347588 62-63 23.24742179051265 26.419470489312 27.16487924679143 23.168228473383916 64-65 23.180024856048508 26.39893847187316 27.071446199271136 23.349590472807193 66-67 23.230135602508867 26.58195180318624 26.797486884851857 23.390425709453044 68-69 23.232968678135606 26.355261486240327 27.073866692282394 23.337903143341673 70-71 23.344497431858937 26.27553809671155 26.933918895170354 23.44604557625916 72-73 23.299207704627612 26.55940267533597 26.69585649617575 23.44553312386067 74-75 23.20282298279482 26.688856141289026 26.63659917601766 23.471721699898495 76-77 23.336415851684297 26.623178763887967 26.58545672277294 23.454948661654804 78-79 23.35879648657673 26.610283917445233 26.64591180016303 23.385007795815007 80-81 23.373981130580404 26.729228299791192 26.50820119884958 23.388589370778828 82-83 23.419088466770457 26.79266180292506 26.57267790718933 23.21557182311515 84-85 23.442339641250403 26.716623587380862 26.386567939184058 23.454468832184673 86-87 23.296898746211134 26.54837761785877 26.657738996162266 23.49698463976783 88-89 23.385190540002302 26.739875443853446 26.405720508082926 23.46921350806133 90-91 23.26121688840971 26.872819166556393 26.51917890094431 23.34678504408958 92-93 23.282593306471966 26.723611105253237 26.523116483778956 23.470679104495833 94-95 23.268832826576837 26.568825810118707 26.61752047223198 23.544820891072476 96-97 23.38848033112546 26.425582540657494 26.544973632807096 23.64096349540995 98-99 23.076490610489707 26.558717142468847 26.462920719606437 23.901871527435006 100-101 23.043664856363186 26.54371034081912 26.531337683462276 23.881287119355417 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 107.0 1 87.0 2 120.0 3 199.0 4 166.5 5 153.5 6 326.5 7 708.5 8 1168.0 9 1352.0 10 1194.5 11 1077.0 12 1161.0 13 1244.0 14 1357.5 15 1527.0 16 1712.0 17 1829.0 18 1953.5 19 2176.5 20 2494.5 21 3005.0 22 3708.0 23 4678.5 24 5827.0 25 7137.5 26 9238.0 27 12085.0 28 14615.5 29 17303.0 30 20813.0 31 24935.0 32 29547.0 33 33216.0 34 36597.5 35 40601.0 36 45726.0 37 50729.0 38 53926.5 39 56754.0 40 60127.5 41 63494.0 42 65844.5 43 67896.5 44 70948.0 45 74988.5 46 78087.5 47 80483.0 48 82941.0 49 84351.5 50 84627.5 51 84930.0 52 85284.0 53 86680.5 54 88460.5 55 85697.0 56 79219.0 57 72548.5 58 67548.5 59 61337.5 60 52230.0 61 43643.0 62 36316.0 63 28914.5 64 21716.0 65 15989.5 66 11472.0 67 8819.0 68 6460.5 69 4336.0 70 3232.0 71 2219.5 72 1593.5 73 1336.5 74 1048.5 75 689.5 76 456.5 77 263.0 78 147.0 79 71.0 80 34.5 81 19.5 82 7.5 83 5.5 84 4.0 85 1.5 86 1.0 87 1.0 88 0.5 89 0.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26892085050700987 2 0.018326457960477712 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.541344533425644E-4 12-13 6.640021000173082E-5 14-15 0.0 16-17 5.312016800138466E-4 18-19 0.0 20-21 4.426680666782055E-5 22-23 4.426680666782055E-5 24-25 0.002722408610070964 26-27 0.002456807770064041 28-29 0.0020805399133875663 30-31 0.001195203780031155 32-33 0.0017264054600450015 34-35 0.0017042720567110913 36-37 6.640021000173082E-5 38-39 1.3280042000346165E-4 40-41 6.197352933494878E-4 42-43 0.004006146003437761 44-45 0.003829078776766478 46-47 0.013789110277026104 48-49 0.014010444310365204 50-51 0.0066621544035069934 52-53 0.007702424360200776 54-55 4.8693487334602615E-4 56-57 0.0032093434834169904 58-59 0.0012616039900328858 60-61 0.0012616039900328858 62-63 0.0 64-65 9.51736343358142E-4 66-67 0.0 68-69 0.0 70-71 8.85336133356411E-5 72-73 0.0010181365533598727 74-75 0.0 76-77 0.1938443463983862 78-79 0.10489019839940081 80-81 0.0013280042000346167 82-83 0.0 84-85 7.082689066851288E-4 86-87 0.12164518472317087 88-89 0.5849637167119147 90-91 0.31088578322810373 92-93 0.2921387906042818 94-95 0.0024125409633962203 96-97 0.002943742643410067 98-99 0.00318721008008308 100-101 6.861355033512186E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2259029.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.25122466905007 #Duplication Level Percentage of deduplicated Percentage of total 1 70.4050274104178 35.37938850231534 2 15.426118100650923 15.503626528942188 3 5.68443015381528 8.569487303246834 4 2.7679678872782905 5.56375104721349 5 1.570181202433763 3.94517641873091 6 0.9638065302701325 2.905947509406122 7 0.6597914886723637 2.3208731232401365 8 0.43158698464951395 1.7350219623888472 9 0.3343530673831744 1.512148599707214 >10 1.612279754373786 14.190725257554798 >50 0.10185313121567784 3.5038916818474557 >100 0.039960775339261456 3.4526057774251653 >500 0.002114810800047157 0.7338070044185218 >1k 4.405855833430263E-4 0.4529836866521748 >5k 8.811711666860527E-5 0.23056559691083814 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5207 0.23049726231934164 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3986 0.17644749137793275 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2567 0.11363289271629537 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.85336133356411E-5 2 0.0 0.0 0.0 0.0 8.85336133356411E-5 3 0.0 0.0 0.0 0.0 8.85336133356411E-5 4 0.0 0.0 0.0 0.0 8.85336133356411E-5 5 0.0 0.0 0.0 0.0 8.85336133356411E-5 6 0.0 0.0 0.0 0.0 8.85336133356411E-5 7 0.0 0.0 0.0 0.0 8.85336133356411E-5 8 0.0 0.0 0.0 0.0 8.85336133356411E-5 9 0.0 0.0 0.0 0.0 8.85336133356411E-5 10-11 0.0 0.0 0.0 0.0 8.85336133356411E-5 12-13 0.0 0.0 0.0 0.0 1.5493382333737194E-4 14-15 0.0 0.0 0.0 0.0 1.992006300051925E-4 16-17 0.0 0.0 0.0 1.5493382333737194E-4 2.2133403333910278E-4 18-19 0.0 0.0 0.0 2.2133403333910278E-4 2.2133403333910278E-4 20-21 0.0 0.0 0.0 3.320010500086542E-4 2.2133403333910278E-4 22-23 0.0 0.0 0.0 6.197352933494878E-4 2.2133403333910278E-4 24-25 0.0 0.0 0.0 0.0010624033600276934 2.2133403333910278E-4 26-27 0.0 0.0 0.0 0.0011509369733633344 2.6560084000692335E-4 28-29 0.0 0.0 0.0 0.001350137603368527 2.6560084000692335E-4 30-31 0.0 0.0 0.0 0.002810942223406605 2.6560084000692335E-4 32-33 0.0 0.0 0.0 0.005887485286820133 2.6560084000692335E-4 34-35 0.0 0.0 0.0 0.009296029400242316 2.6560084000692335E-4 36-37 0.0 0.0 0.0 0.015227781493730271 2.6560084000692335E-4 38-39 0.0 0.0 0.0 0.025586214254000282 3.098676466747439E-4 40-41 0.0 0.0 0.0 0.03806945373432567 3.098676466747439E-4 42-43 0.0 0.0 0.0 0.0510396280879971 3.762678566764747E-4 44-45 0.0 0.0 0.0 0.06560340748171006 3.98401260010385E-4 46-47 0.0 0.0 0.0 0.08497013539888155 4.4266806667820556E-4 48-49 0.0 0.0 0.0 0.10674940427944926 4.4266806667820556E-4 50-51 0.0 0.0 0.0 0.13107401454341666 4.4266806667820556E-4 52-53 0.0 0.0 0.0 0.15557569203405536 4.869348733460261E-4 54-55 0.0 0.0 0.0 0.1804315039780366 4.869348733460261E-4 56-57 0.0 0.0 0.0 0.21261347242554213 4.869348733460261E-4 58-59 0.0 0.0 0.0 0.24975332321984356 5.090682766799364E-4 60-61 0.0 0.0 0.0 0.28954918241421423 5.312016800138467E-4 62-63 0.0 0.0 0.0 0.33111571387529776 6.640021000173084E-4 64-65 0.0 0.0 0.0 0.37339051424306635 7.304023100190391E-4 66-67 0.0 0.0 0.0 0.4208666643943039 7.525357133529494E-4 68-69 0.0 0.0 0.0 0.4811359216725416 7.525357133529494E-4 70-71 0.0 0.0 0.0 0.549351070747653 7.525357133529494E-4 72-73 0.0 0.0 0.0 0.6204878290628407 8.410693266885905E-4 74-75 0.0 0.0 0.0 0.6919787218313709 8.853361333564111E-4 76-77 0.0 0.0 0.0 0.7708400379100933 8.853361333564111E-4 78-79 0.0 0.0 0.0 0.8648184684658763 8.853361333564111E-4 80-81 0.0 0.0 0.0 0.9725196090886836 9.074695366903214E-4 82-83 0.0 0.0 0.0 1.084669563781607 9.738697466920522E-4 84-85 0.0 0.0 0.0 1.2021758020813367 9.738697466920522E-4 86-87 0.0 0.0 0.0 1.3266097956245804 0.001018136553359873 88-89 0.0 0.0 0.0 1.467001972971573 0.0010845367633616036 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3320 0.0 53.890846 1 GTATCAA 6130 0.0 40.753426 1 ATCAACG 7555 0.0 32.272068 3 TCAACGC 7605 0.0 32.24701 4 CAACGCA 7755 0.0 31.623278 5 TATCAAC 7875 0.0 31.210613 2 AACGCAG 8045 0.0 30.66023 6 GTACATG 8915 0.0 26.260866 1 TACATGG 8870 0.0 26.15823 2 ACGCAGA 9625 0.0 25.52861 7 TGGTATC 1700 0.0 25.398941 2 ACATGGG 8780 0.0 24.797981 3 CGCAGAG 9715 0.0 24.75502 8 GTACCTG 2040 0.0 24.025494 1 GTGGTAT 1990 0.0 23.911797 1 TACCTGG 2040 0.0 23.259102 2 GCAGAGT 10915 0.0 21.468483 9 GAGTACA 6545 0.0 20.356993 1 CATGGGG 5465 0.0 20.050257 4 TACGCTC 145 6.205589E-4 19.62824 4 >>END_MODULE