FastQCFastQC Report
Thu 26 May 2016
SRR936301_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936301_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2189577
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53730.24538986297353324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37770.17249907173851386No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG30500.13929631157068237No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG29670.13550562505908675No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24040.1097928960707936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55000.033.3130871
GTATCAA85950.033.1051251
GTACATG106500.029.8290581
TACATGG110500.028.3565372
ATCAACG100500.027.880673
ACATGGG109700.027.7860493
TCAACGC101250.027.6267684
TATCAAC103900.027.151152
CAACGCA104500.026.8128555
TATAACG1955.5842975E-1026.7002662
AACGCAG106200.026.381846
CATGGGG52400.023.7553864
ACGCAGA123400.022.5895737
CGCAGAG124650.022.2876198
GAGTACT53350.021.73598112-13
TAACGCA2205.8631485E-821.5136934
CATGGGA59550.020.9826244
AGTACAT75600.020.9739632
GTACCTG19800.020.5636351
GCAGAGT132000.020.4724629
Produced by FastQC (version 0.11.5)