##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936301_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2189577 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.847898475367618 33.0 31.0 34.0 30.0 34.0 2 31.936607847086446 34.0 31.0 34.0 30.0 34.0 3 32.006819125337906 34.0 31.0 34.0 30.0 34.0 4 35.51162165112257 37.0 35.0 37.0 33.0 37.0 5 35.4961693514318 37.0 35.0 37.0 33.0 37.0 6 35.59467056879023 37.0 35.0 37.0 33.0 37.0 7 35.54045872787301 37.0 35.0 37.0 33.0 37.0 8 35.546648507908145 37.0 35.0 37.0 33.0 37.0 9 37.22661089333693 39.0 37.0 39.0 34.0 39.0 10-11 37.14657602815521 39.0 37.0 39.0 34.0 39.0 12-13 37.05380810996827 39.0 37.0 39.0 33.0 39.0 14-15 38.31683128750439 40.0 38.0 41.0 33.5 41.0 16-17 38.26600343353991 40.0 38.0 41.0 33.0 41.0 18-19 38.21473417011596 40.0 38.0 41.0 33.5 41.0 20-21 38.147039131302535 40.0 38.0 41.0 33.5 41.0 22-23 37.992127474850165 40.0 38.0 41.0 33.0 41.0 24-25 38.141708192952336 40.0 38.0 41.0 33.5 41.0 26-27 38.09837607903262 40.0 38.0 41.0 33.0 41.0 28-29 37.969348417525396 40.0 38.0 41.0 33.0 41.0 30-31 37.82490225280956 40.0 38.0 41.0 33.0 41.0 32-33 37.655749946222485 40.0 37.5 41.0 33.0 41.0 34-35 37.399757350392335 40.0 37.0 41.0 32.0 41.0 36-37 37.199786077402166 40.0 37.0 41.0 31.0 41.0 38-39 37.0444042388096 40.0 37.0 41.0 31.0 41.0 40-41 36.854344925983426 40.0 36.5 41.0 31.0 41.0 42-43 36.664320551412445 40.0 36.0 41.0 30.0 41.0 44-45 36.47902357395972 40.0 36.0 41.0 30.0 41.0 46-47 36.182936932567344 39.5 35.5 41.0 29.5 41.0 48-49 35.99799139285807 39.0 35.0 41.0 29.0 41.0 50-51 35.679188720013045 39.0 35.0 40.5 28.5 41.0 52-53 35.68706101680827 39.0 35.0 40.5 28.0 41.0 54-55 35.75282919942984 39.0 35.0 41.0 28.0 41.0 56-57 35.5842927652236 39.0 35.0 41.0 28.0 41.0 58-59 35.34920238018576 39.0 35.0 41.0 28.0 41.0 60-61 35.049761437939836 38.0 35.0 40.5 27.0 41.0 62-63 34.74112419887494 37.5 34.0 40.0 26.5 41.0 64-65 34.4041673346039 37.0 34.0 40.0 26.0 41.0 66-67 34.05100551385039 36.5 34.0 39.5 26.0 41.0 68-69 33.67801771757742 36.0 34.0 39.0 26.0 41.0 70-71 33.28160599056348 35.5 33.5 39.0 25.0 40.5 72-73 32.89132010429412 35.0 33.0 37.5 24.5 40.0 74-75 32.484933162889455 35.0 33.0 37.0 23.5 39.0 76-77 32.09318101167486 35.0 33.0 36.5 22.5 39.0 78-79 31.719397399589052 35.0 32.0 36.0 21.5 38.5 80-81 31.34802155850194 35.0 32.0 36.0 20.0 37.0 82-83 31.023930421264016 35.0 32.0 35.0 19.0 37.0 84-85 30.73692498596761 35.0 31.5 35.0 18.0 36.5 86-87 30.42440366335598 34.5 31.0 35.0 15.5 36.0 88-89 30.127642005739006 34.0 31.0 35.0 8.5 36.0 90-91 29.804058044087967 34.0 31.0 35.0 4.5 35.5 92-93 29.517779233157817 34.0 30.5 35.0 2.0 35.0 94-95 29.166473478667342 34.0 30.0 35.0 2.0 35.0 96-97 28.767755598455775 34.0 30.0 35.0 2.0 35.0 98-99 28.302686317950908 34.0 29.5 35.0 2.0 35.0 100-101 25.06527196805593 30.5 23.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3460232875709295 1101 2 0.24492898998684254 1101 3 0.29126519833817355 1101 4 0.17722781481928962 1101 5 0.4082370318827131 1101 6 0.32238522769124245 1101 7 0.34899850102932106 1101 8 0.3770429603679659 1101 9 0.4146220506852387 1101 10-11 0.5373455509918443 1101 12-13 0.47398689743683775 1101 14-15 0.6788960404903008 1101 16-17 0.706802346129173 1101 18-19 0.6380383049829206 1101 20-21 0.6808529239131076 1101 22-23 0.69412265270212 1101 24-25 0.62016556880846 1101 26-27 0.5926491165887597 1101 28-29 0.5960772619148074 1101 30-31 0.674205056497108 1101 32-33 0.5745527428068158 1101 34-35 0.3981981548183313 1101 36-37 0.4032290270051817 1101 38-39 0.5013876278206126 1101 40-41 0.452418969681581 1101 42-43 0.48911205077368436 1101 44-45 0.4526502102399448 1101 46-47 0.436681545415432 1101 48-49 0.28366558412426457 1101 50-51 0.6091718584537631 1101 52-53 0.5918418345969414 1101 54-55 0.5976744759708268 1101 56-57 0.6378350444017116 1101 58-59 0.665420682858958 1101 60-61 0.6157233760099601 1101 62-63 0.6116548116324694 1101 64-65 0.5676875830392802 1101 66-67 0.6706545499867147 1101 68-69 0.6314494654550629 1101 70-71 0.7099775846416563 1101 72-73 0.7980448820401946 1101 74-75 0.6055715548344125 1101 76-77 0.6115170611241112 1101 78-79 0.7232435534110486 1101 80-81 0.7358208935713471 1101 82-83 0.7737990674729609 1101 84-85 0.7862641756662576 1101 86-87 0.6478445420262879 1101 88-89 0.6142370385526839 1101 90-91 0.6215137028277411 1101 92-93 0.6031262699878468 1101 94-95 0.9010513461787006 1101 96-97 0.923717170189331 1101 98-99 0.8963590351245259 1101 100-101 1.2769375195428303 1102 1 0.2338324239384164 1102 2 0.2177990072199414 1102 3 0.16333091012165823 1102 4 0.1670828872830583 1102 5 0.25072170572512675 1102 6 0.20989264357394433 1102 7 0.11162951495705897 1102 8 0.17810915241813774 1102 9 0.21630326660748977 1102 10-11 0.2079326063920206 1102 12-13 0.32460601770126374 1102 14-15 0.4494592238109192 1102 16-17 0.3839739990941169 1102 18-19 0.37500866970309943 1102 20-21 0.2649510113216422 1102 22-23 0.24243380901479838 1102 24-25 0.3958822962147366 1102 26-27 0.31811963668933174 1102 28-29 0.22467686075086846 1102 30-31 0.2705020590679439 1102 32-33 0.08988059544969218 1102 34-35 0.14102904444174413 1102 36-37 0.19544333895252208 1102 38-39 0.19839938022901293 1102 40-41 0.24707812158711562 1102 42-43 0.1977017617661403 1102 44-45 0.0633419355273972 1102 46-47 0.11928812224397944 1102 48-49 0.10971341303631732 1102 50-51 0.01652163385845995 1102 52-53 0.08261605275102823 1102 54-55 0.11047089939653887 1102 56-57 -0.014507103237917818 1102 58-59 0.04765533551313439 1102 60-61 0.09838956240993468 1102 62-63 0.10216121047982796 1102 64-65 0.10928404601636288 1102 66-67 0.14340125168459394 1102 68-69 0.07143971886637246 1102 70-71 0.2886882098358825 1102 72-73 0.26490958808872733 1102 74-75 0.29335399402417295 1102 76-77 0.19790432558155402 1102 78-79 0.2357687674122353 1102 80-81 0.10474297736375959 1102 82-83 0.23493602823235094 1102 84-85 0.2845271640551026 1102 86-87 0.24107122790362467 1102 88-89 0.06250327580442416 1102 90-91 0.1744275090825056 1102 92-93 0.19263611545497383 1102 94-95 0.24536046842070292 1102 96-97 0.1422238232955273 1102 98-99 -0.02321197371803052 1102 100-101 0.007508754393239059 1103 1 0.19501219014000526 1103 2 0.19141749561083188 1103 3 0.2632721911645888 1103 4 0.127778895471387 1103 5 0.22588897394685858 1103 6 0.16709783670005862 1103 7 0.24581310597144324 1103 8 0.24704413060054264 1103 9 0.28309257867989146 1103 10-11 0.35879291991892615 1103 12-13 0.3149720684473678 1103 14-15 0.3063182983139612 1103 16-17 0.4193667157886196 1103 18-19 0.2607135665408933 1103 20-21 0.35934415898096717 1103 22-23 0.3975929966461109 1103 24-25 0.4046467739392341 1103 26-27 0.3391200137835497 1103 28-29 0.32497530389044016 1103 30-31 0.3300358090006057 1103 32-33 0.4693725956651704 1103 34-35 0.3533969267883492 1103 36-37 0.3067513171887981 1103 38-39 0.3236417979416686 1103 40-41 0.44815284011320955 1103 42-43 0.33626772631929214 1103 44-45 0.4838695980819452 1103 46-47 0.5662242317090431 1103 48-49 0.5834065122121004 1103 50-51 0.6022114469274698 1103 52-53 0.6676580718212151 1103 54-55 0.7531905567795079 1103 56-57 0.7189651264663297 1103 58-59 0.6587451378080189 1103 60-61 0.759779693315501 1103 62-63 0.6583512120959512 1103 64-65 0.7083488523515555 1103 66-67 0.8052212700005938 1103 68-69 0.680058207257261 1103 70-71 0.6554403511923468 1103 72-73 0.6593399064639911 1103 74-75 0.6739840107240553 1103 76-77 0.6821256260178998 1103 78-79 0.6757679593531911 1103 80-81 0.6702191934926169 1103 82-83 0.7376788499745253 1103 84-85 0.680641208121255 1103 86-87 0.760052265451435 1103 88-89 0.7605790178719793 1103 90-91 0.9277157706781551 1103 92-93 0.8697346447875169 1103 94-95 0.8329519137689729 1103 96-97 0.9478091273950788 1103 98-99 1.1086010414196075 1103 100-101 1.1531549322739671 1104 1 0.28225401610438183 1104 2 0.23009067950676254 1104 3 0.2960221076182137 1104 4 0.3122904731364642 1104 5 0.38285070839582147 1104 6 0.28055276389011397 1104 7 0.34240320726060247 1104 8 0.32671904208335434 1104 9 0.32390072000521286 1104 10-11 0.37806695045309624 1104 12-13 0.44014447301433535 1104 14-15 0.4951948789405449 1104 16-17 0.47969913330329206 1104 18-19 0.3545446701347217 1104 20-21 0.45142380530043624 1104 22-23 0.492322125397763 1104 24-25 0.5419082208417763 1104 26-27 0.5431065529805679 1104 28-29 0.49951351671676747 1104 30-31 0.5461468026542278 1104 32-33 0.6159773339643593 1104 34-35 0.53478541769163 1104 36-37 0.5277352810065281 1104 38-39 0.48777527383283115 1104 40-41 0.4625317351186311 1104 42-43 0.48266628738053896 1104 44-45 0.5843450648268131 1104 46-47 0.42341488584698794 1104 48-49 0.3806040494171228 1104 50-51 0.41228574942672225 1104 52-53 0.44195957429193555 1104 54-55 0.653835031150841 1104 56-57 0.5780374757903743 1104 58-59 0.6206430071525091 1104 60-61 0.6196338635780236 1104 62-63 0.6792732178175029 1104 64-65 0.7416445089368651 1104 66-67 0.7163933744826139 1104 68-69 0.608659633080407 1104 70-71 0.5646940368072109 1104 72-73 0.6451472529839251 1104 74-75 0.5646185277315823 1104 76-77 0.6559703645898836 1104 78-79 0.7123304496804401 1104 80-81 0.6726138398094683 1104 82-83 0.8893614241157017 1104 84-85 0.5809027069615773 1104 86-87 0.5558958792295279 1104 88-89 0.5569984065066294 1104 90-91 0.6677850315406246 1104 92-93 0.747418817982691 1104 94-95 0.35715819698095075 1104 96-97 0.6950722950643886 1104 98-99 0.7698020002224588 1104 100-101 0.7479573772317316 1105 1 0.04199377777802127 1105 2 0.035395729054865654 1105 3 0.04984413453760084 1105 4 0.016122321054538702 1105 5 0.029802648729756243 1105 6 0.09793479141067962 1105 7 0.09334682643595471 1105 8 0.07284493018408966 1105 9 0.1142687820138022 1105 10-11 0.13466295602747635 1105 12-13 0.15006261926584585 1105 14-15 0.10688166008360866 1105 16-17 0.10937259416622425 1105 18-19 0.10979553575599965 1105 20-21 0.09636179307887005 1105 22-23 0.08842603933782556 1105 24-25 0.14914795530685154 1105 26-27 0.03764281267783787 1105 28-29 0.0657779197684718 1105 30-31 0.25720446979648415 1105 32-33 0.13231154643686693 1105 34-35 -0.03391933510165046 1105 36-37 0.059219614829267186 1105 38-39 0.11090403671349947 1105 40-41 -0.03234553867577716 1105 42-43 0.10927645177731904 1105 44-45 0.14022900912646463 1105 46-47 0.04269959582175176 1105 48-49 -0.014500040212574561 1105 50-51 -0.011104889477181246 1105 52-53 0.08636492189050671 1105 54-55 0.15447520997074804 1105 56-57 0.2811990858951745 1105 58-59 0.23442666221208697 1105 60-61 0.1626594256423317 1105 62-63 -0.13363597966438334 1105 64-65 -0.06878896766038878 1105 66-67 -0.08965952209008066 1105 68-69 0.11554301299653957 1105 70-71 0.2044132909917522 1105 72-73 0.1977721286666707 1105 74-75 0.14389121929158932 1105 76-77 0.16075622276637347 1105 78-79 0.14762470908644687 1105 80-81 0.07978661527552333 1105 82-83 0.12773869972401997 1105 84-85 0.05916422434992441 1105 86-87 0.08132267806593418 1105 88-89 0.044697411294819744 1105 90-91 -0.034114878230568024 1105 92-93 -0.05791680881728212 1105 94-95 -0.1535555019906667 1105 96-97 -0.11932106521492969 1105 98-99 0.09342178712492455 1105 100-101 0.2792031825769463 1106 1 0.18586726823448885 1106 2 0.21935135190723187 1106 3 0.11902284064171198 1106 4 0.20642877429990136 1106 5 0.14678920350823432 1106 6 0.1437354269446658 1106 7 0.18228049821301795 1106 8 0.20186273856388226 1106 9 0.06927233447041203 1106 10-11 0.1522959677190201 1106 12-13 0.07942084217572898 1106 14-15 0.13256782534448774 1106 16-17 0.13809037278354452 1106 18-19 0.06677122773345445 1106 20-21 0.0745848332401664 1106 22-23 0.16563760641712122 1106 24-25 0.1311890094795558 1106 26-27 0.19118885582239642 1106 28-29 0.25988785652572943 1106 30-31 0.21309112588762247 1106 32-33 0.18432898103208828 1106 34-35 0.0974681973289151 1106 36-37 0.19699881336700287 1106 38-39 0.18037084754944033 1106 40-41 0.15728624844538786 1106 42-43 0.25418812137179714 1106 44-45 0.22118894402982647 1106 46-47 0.25217503476810066 1106 48-49 0.3608638485900215 1106 50-51 0.2848811351885914 1106 52-53 0.28173425211465286 1106 54-55 0.2862147312258614 1106 56-57 0.37609928140555127 1106 58-59 0.35053311966628087 1106 60-61 0.4515816233352865 1106 62-63 0.2891068575509266 1106 64-65 0.3414957198987949 1106 66-67 0.3148688329270968 1106 68-69 0.3321248683108422 1106 70-71 0.33010962760230456 1106 72-73 0.443226838101765 1106 74-75 0.2912307349349206 1106 76-77 0.4104623559399059 1106 78-79 0.4416518721016125 1106 80-81 0.47068085227899203 1106 82-83 0.47162451605249345 1106 84-85 0.4028493694695321 1106 86-87 0.46112312921289345 1106 88-89 0.3834547128708259 1106 90-91 0.5168290530812563 1106 92-93 0.46311809344256005 1106 94-95 0.49557276824856444 1106 96-97 0.45453721331169916 1106 98-99 0.49484211840027115 1106 100-101 0.48642013095158276 1107 1 -0.08674158835790635 1107 2 -0.041285109567571254 1107 3 0.012054959637801232 1107 4 -0.03055985760956048 1107 5 -0.12744514933814344 1107 6 0.014273553943112915 1107 7 -0.05816315687443563 1107 8 0.033679167030364 1107 9 0.07892822869841609 1107 10-11 0.059711989370157426 1107 12-13 0.015428024262980955 1107 14-15 0.0760789751274018 1107 16-17 -0.15206953430722336 1107 18-19 0.0346618361446005 1107 20-21 0.020271791007893114 1107 22-23 0.0011801904214294723 1107 24-25 0.050249835019840816 1107 26-27 0.04219286323395721 1107 28-29 0.0836766126187456 1107 30-31 0.1383062276875009 1107 32-33 0.19089573150880312 1107 34-35 0.023294352814822616 1107 36-37 0.10460134397030885 1107 38-39 -0.03970750957275726 1107 40-41 0.0622431294446244 1107 42-43 -0.21645417548400303 1107 44-45 -0.005960399671515404 1107 46-47 0.05872527589289689 1107 48-49 0.049087982251393214 1107 50-51 0.04161661331863087 1107 52-53 0.042950617554858184 1107 54-55 0.1821379336663469 1107 56-57 0.20652526421817186 1107 58-59 0.2653146225468461 1107 60-61 0.3512581075286221 1107 62-63 0.45079882730870224 1107 64-65 0.27613166532655953 1107 66-67 0.24600264122444315 1107 68-69 0.36838755598142825 1107 70-71 0.349944861155123 1107 72-73 0.2715201033764245 1107 74-75 0.3373780076990087 1107 76-77 0.2864034019786672 1107 78-79 0.20379377361554063 1107 80-81 0.23547813768467662 1107 82-83 0.4566366981890617 1107 84-85 0.3679920533606058 1107 86-87 0.17197649657556724 1107 88-89 0.22533198193329085 1107 90-91 0.47257010233882824 1107 92-93 0.16199621029658573 1107 94-95 0.3732331490746681 1107 96-97 0.4086035757148174 1107 98-99 0.46797561416209277 1107 100-101 0.6892437492927463 1108 1 -0.03844630659578385 1108 2 -0.03769478343647137 1108 3 -0.092674412172844 1108 4 -0.09518303031993725 1108 5 -0.08423689865098538 1108 6 -0.14966284627426774 1108 7 -0.07603557473346001 1108 8 -0.0888058879918745 1108 9 -0.040914356100962834 1108 10-11 -0.10014298990208914 1108 12-13 -0.08811278773929843 1108 14-15 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-1.2206673552820249 2316 76-77 -1.5495251203128895 2316 78-79 -1.4950695261454179 2316 80-81 -1.3804990565472757 2316 82-83 -1.4339068468826603 2316 84-85 -1.5867801758251012 2316 86-87 -1.5091314684555392 2316 88-89 -1.6325448868784136 2316 90-91 -1.7523274714834258 2316 92-93 -1.835722222416102 2316 94-95 -1.742177507807778 2316 96-97 -2.060273125462384 2316 98-99 -1.921855250658151 2316 100-101 -1.7789029849086369 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14474.0 3 3320.0 4 2854.0 5 2504.0 6 2333.0 7 2543.0 8 3049.0 9 3558.0 10 4200.0 11 5274.0 12 7064.0 13 10571.0 14 10682.0 15 9130.0 16 11035.0 17 14501.0 18 13001.0 19 9099.0 20 9099.0 21 9613.0 22 10088.0 23 10941.0 24 12565.0 25 14656.0 26 17533.0 27 20946.0 28 25678.0 29 31379.0 30 39199.0 31 49092.0 32 62935.0 33 83709.0 34 118096.0 35 181084.0 36 302545.0 37 496358.0 38 480746.0 39 84071.0 40 52.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.51580192116928 17.72258783503148 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25.964315925829162 27.28602527538181 23.073857501219557 20-21 24.177657344781267 25.48132981534199 27.200552453132143 23.140460386744596 22-23 23.89852521602661 25.29721429186413 27.23774836751095 23.566512124598315 24-25 23.762239423333874 25.600225659906766 27.339337226804194 23.298197689955167 26-27 23.79940696568677 25.8555469992482 27.243746968372417 23.10129906669261 28-29 23.269475678697894 25.790291577897236 27.593308398715145 23.34692434468973 30-31 23.53971200882852 25.575803059332376 27.433638222081942 23.450846709757165 32-33 23.543328342406227 25.705611732667794 27.491702850126938 23.259357074799045 34-35 23.83472718372311 25.73042629704102 27.118399969960056 23.316446549275813 36-37 24.13875700728944 25.6833261557826 26.905100542687077 23.27281629424088 38-39 23.71197338610227 25.66219851443976 27.092947890676534 23.53288020878144 40-41 23.636302721095458 25.60598122845944 27.41190614367404 23.34580990677106 42-43 23.879077489860485 25.591643882009897 26.906981696096278 23.622296932033347 44-45 23.55299773311737 25.74915215652184 26.95651156183578 23.74133854852501 46-47 23.82226763267928 26.062764197863213 26.56314367659003 23.551824492867475 48-49 24.26107645125737 25.880766022805883 26.371912803142383 23.48624472279436 50-51 23.840005986473507 26.019467975121245 26.347874710087577 23.79265132831767 52-53 24.03947869431669 26.06576615056795 26.274603320692325 23.62015183442304 54-55 23.847421846690704 25.78231334667567 26.302788466106126 24.067476340527502 56-57 23.746165454114873 26.229372392003953 26.297985750451154 23.726476403430024 58-59 24.07275334063969 25.816412137366886 26.29063703002032 23.8201974919731 60-61 24.09616298942445 25.812579050458282 26.623551850675636 23.46770610944163 62-63 23.859975438635136 25.89344718205148 26.63586399832586 23.610713380987523 64-65 23.737645189392236 25.91503855958537 26.506628246307674 23.840688004714725 66-67 23.800409815096806 26.110810679971397 26.188084067388452 23.900695437543348 68-69 23.284881445987853 25.990726998626005 26.647566508414112 24.076825046972022 70-71 23.58120645951947 25.864327512753672 26.610491544935165 23.943974482791695 72-73 23.682878969824323 26.125108168583967 26.24468737644228 23.947325485149438 74-75 23.507947728138586 26.339822180197466 26.14419285691335 24.0080372347506 76-77 23.724149040677936 26.233800088438773 26.126177395700644 23.915873475182643 78-79 23.720388782617317 26.04217533218536 26.29583497024192 23.94160091495541 80-81 23.58719049039631 26.681230924570297 26.16437570277899 23.5672028822544 82-83 23.718073020734394 26.52759900487397 26.044906427540234 23.709421546851402 84-85 23.835643095852067 26.08531224190581 25.858562261469046 24.220482400773076 86-87 23.64515458343641 25.92520347293522 26.412813344648477 24.01682859897989 88-89 23.805780525126295 26.189020954512603 26.109700484896862 23.89549803546424 90-91 23.84481226427288 26.303433681432946 26.1965909975282 23.655163056765975 92-93 23.714767034847277 26.09802851714474 26.151305633670585 24.035898814337397 94-95 23.708654133662986 26.084047142990567 26.263108629487753 23.944190093858694 96-97 23.759384765880405 26.093835958674223 26.031412324962616 24.115366950482755 98-99 23.707987470364845 26.01179327248974 25.95125554422592 24.328963712919492 100-101 23.734370400992717 26.16176300890328 25.665084560366164 24.438782029737848 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 191.0 1 136.0 2 186.5 3 316.5 4 261.5 5 270.5 6 544.5 7 990.5 8 1585.5 9 1829.0 10 1573.5 11 1473.5 12 1653.0 13 1913.0 14 2150.5 15 2338.5 16 2552.0 17 2738.0 18 2794.5 19 2788.5 20 2855.0 21 2991.0 22 3216.5 23 3587.0 24 4158.5 25 4992.0 26 6200.0 27 7682.0 28 9460.0 29 11925.5 30 15205.5 31 19079.5 32 22909.5 33 27066.0 34 31732.5 35 35502.5 36 40326.0 37 45546.5 38 49217.5 39 52821.5 40 55519.5 41 60123.5 42 65048.0 43 69576.5 44 74178.0 45 77114.5 46 78192.0 47 77537.0 48 81322.0 49 82280.0 50 79174.5 51 78314.0 52 80005.0 53 83880.5 54 86060.0 55 82833.5 56 75412.0 57 69496.5 58 66738.5 59 62005.5 60 54330.0 61 46435.0 62 39781.5 63 33443.5 64 26806.0 65 20875.0 66 15152.0 67 12328.0 68 8717.5 69 4892.5 70 3760.0 71 3076.0 72 2602.0 73 2385.0 74 1827.5 75 1302.0 76 885.5 77 478.5 78 282.0 79 164.0 80 93.0 81 68.0 82 55.0 83 37.5 84 24.5 85 23.5 86 23.0 87 20.0 88 22.5 89 20.5 90 20.5 91 21.5 92 20.5 93 22.0 94 20.5 95 19.0 96 21.0 97 19.0 98 11.5 99 7.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046310314732023586 2 0.012239807049489468 3 0.010686995707390057 4 0.005937219837438921 5 0.008723146068852567 6 0.005617523384653748 7 0.00493245955725695 8 0.008768816990679022 9 0.01342725101697725 10-11 0.002215039708582982 12-13 0.008038082241455769 14-15 0.008152259546021903 16-17 0.018199862347841614 18-19 0.011554743222092668 20-21 0.0031969645278517264 22-23 0.00938537443533614 24-25 0.02018654744729233 26-27 0.007261676570406064 28-29 0.017583304903184497 30-31 0.014089479383460824 32-33 0.012376819814968826 34-35 0.2657134231863049 36-37 0.4717121160845223 38-39 0.4687891770876293 40-41 0.4883820025511777 42-43 0.4785855898194035 44-45 0.4938853486312653 46-47 0.4992060110240471 48-49 0.5071299159609368 50-51 0.5175885570591945 52-53 0.5062850039071474 54-55 0.4931774493429553 56-57 0.4887245344648761 58-59 0.4865323302172064 60-61 0.4836093912203133 62-63 0.48159987065994936 64-65 0.49648859117537314 66-67 0.470479001195208 68-69 0.49719649046368314 70-71 0.4794761727950193 72-73 0.4880623060983925 74-75 0.5008729996707126 76-77 0.4872402295055164 78-79 0.4874457486537354 80-81 0.48991197843236384 82-83 0.4913049415480707 84-85 0.49075689048615323 86-87 0.49260656282012466 88-89 0.5056912819234035 90-91 0.5301708960223823 92-93 0.5180681017383724 94-95 0.4894324337531861 96-97 0.46191570335274806 98-99 0.45417448210316425 100-101 0.46120780406443806 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2189577.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.95954545669785 #Duplication Level Percentage of deduplicated Percentage of total 1 65.91605617021844 24.36227474349437 2 14.425314821813974 10.663061577680216 3 6.447880699394597 7.149322194259179 4 3.596764698100305 5.317391534259373 5 2.209624017718054 4.083334966253088 6 1.5048368008351247 3.3370850487226535 7 1.0434495839173084 2.699579562999495 8 0.7857154508253756 2.3231748736648257 9 0.5695917738497105 1.8946667751623587 >10 3.1415017413016506 20.839962968598375 >50 0.22315301733380621 5.732031575631956 >100 0.12817961835724506 8.46366895395247 >500 0.005329048005941906 1.2537597683433936 >1k 0.0024786269795078627 1.6345780758842008 >5k 1.2393134897539313E-4 0.24610738109414926 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5373 0.24538986297353324 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3777 0.17249907173851386 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 3050 0.13929631157068237 No Hit CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG 2967 0.13550562505908675 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2404 0.1097928960707936 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.567092182645324E-5 0.0 5 0.0 0.0 0.0 4.567092182645324E-5 0.0 6 0.0 0.0 0.0 4.567092182645324E-5 0.0 7 0.0 0.0 0.0 4.567092182645324E-5 0.0 8 0.0 0.0 0.0 4.567092182645324E-5 0.0 9 0.0 0.0 0.0 4.567092182645324E-5 0.0 10-11 0.0 0.0 0.0 4.567092182645324E-5 0.0 12-13 0.0 0.0 0.0 4.567092182645324E-5 0.0 14-15 9.134184365290648E-5 0.0 0.0 4.567092182645324E-5 0.0 16-17 1.141773045661331E-4 0.0 0.0 1.5984822639258635E-4 0.0 18-19 1.5984822639258635E-4 0.0 0.0 2.0551914821903956E-4 0.0 20-21 1.8268368730581296E-4 0.0 0.0 5.023801400909857E-4 0.0 22-23 1.8268368730581296E-4 0.0 0.0 0.001073266662921651 0.0 24-25 2.283546091322662E-4 0.0 0.0 0.0015984822639258634 0.0 26-27 2.283546091322662E-4 0.0 0.0 0.0018040014121449028 0.0 28-29 2.9686099187194604E-4 0.0 0.0 0.0023292170131491152 0.0 30-31 3.1969645278517265E-4 0.0 0.0 0.004955295018170176 0.0 32-33 3.1969645278517265E-4 0.0 0.0 0.010093273723646165 0.0 34-35 3.653673746116259E-4 0.0 0.0 0.015847809873779276 0.0 36-37 3.653673746116259E-4 0.0 0.0 0.024707968708111202 0.0 38-39 4.567092182645324E-4 0.0 0.0 0.0416975516275518 0.0 40-41 4.79544679177759E-4 0.0 0.0 0.06131321255201347 0.0 42-43 5.023801400909857E-4 0.0 0.0 0.08015246780542543 0.0 44-45 5.937219837438921E-4 0.0 0.0 0.1037186634678753 0.0 46-47 6.850638273967985E-4 0.0 0.0 0.13294805343680538 0.0 48-49 8.220765928761583E-4 0.0 0.0 0.16537440793358715 0.0 50-51 8.220765928761583E-4 0.0 0.0 0.20083787873182812 0.0 52-53 8.220765928761583E-4 0.0 0.0 0.23867623746504463 0.0 54-55 8.677475147026115E-4 0.0 0.0 0.27612639336273626 0.0 56-57 0.0010047602801819713 0.0 0.0 0.3239895194368593 0.0 58-59 0.0010047602801819713 0.0 0.0 0.37552915471801174 0.0 60-61 0.0010047602801819713 0.0 0.0 0.43538089777157873 0.0 62-63 0.0010047602801819713 0.0 0.0 0.49877213726669584 0.0 64-65 0.0010047602801819713 0.0 0.0 0.5625515795973377 0.0 66-67 0.0010504312020084244 0.0 0.0 0.6365156374952787 0.0 68-69 0.0010504312020084244 0.0 0.0 0.7187689677047211 0.0 70-71 0.0010504312020084244 0.0 0.0 0.8162763858041987 0.0 72-73 0.0010504312020084244 0.0 0.0 0.9136696265991102 0.0 74-75 0.0010504312020084244 0.0 0.0 1.0154929468111877 0.0 76-77 0.0010504312020084244 0.0 0.0 1.1294647322291018 0.0 78-79 0.0010504312020084244 0.0 0.0 1.2626868111968659 0.0 80-81 0.0013016212720539174 0.0 0.0 1.4139717397469922 0.0 82-83 0.001324456732967144 0.0 0.0 1.5719702938056073 0.0 84-85 0.001324456732967144 0.0 0.0 1.7320468748073259 0.0 86-87 0.001324456732967144 0.0 0.0 1.905482200443282 0.0 88-89 0.001324456732967144 0.0 0.0 2.0997891373539272 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5500 0.0 33.313087 1 GTATCAA 8595 0.0 33.105125 1 GTACATG 10650 0.0 29.829058 1 TACATGG 11050 0.0 28.356537 2 ATCAACG 10050 0.0 27.88067 3 ACATGGG 10970 0.0 27.786049 3 TCAACGC 10125 0.0 27.626768 4 TATCAAC 10390 0.0 27.15115 2 CAACGCA 10450 0.0 26.812855 5 TATAACG 195 5.5842975E-10 26.700266 2 AACGCAG 10620 0.0 26.38184 6 CATGGGG 5240 0.0 23.755386 4 ACGCAGA 12340 0.0 22.589573 7 CGCAGAG 12465 0.0 22.287619 8 GAGTACT 5335 0.0 21.735981 12-13 TAACGCA 220 5.8631485E-8 21.513693 4 CATGGGA 5955 0.0 20.982624 4 AGTACAT 7560 0.0 20.973963 2 GTACCTG 1980 0.0 20.563635 1 GCAGAGT 13200 0.0 20.472462 9 >>END_MODULE