FastQCFastQC Report
Thu 26 May 2016
SRR936301_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936301_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2189577
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57590.2630188387985442No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43810.20008430852169165No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG32990.15066837110546924No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG31690.14473115126803032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28620.13071017826730916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56450.032.876071
GTATCAA86700.032.547291
GTACATG111100.028.8685551
TACATGG114300.027.355642
ACATGGG112450.026.9147013
ATCAACG105750.026.4667133
TCAACGC107550.026.0678624
TATCAAC109400.025.7184682
CAACGCA110400.025.3949185
AACGCAG111600.025.1643586
CATGGGG54650.023.8710164
ACGCAGA126900.022.167747
CGCAGAG128650.021.7555778
GAGTACT55400.021.36432312-13
CATGGGA60300.021.0836524
GCAGAGT137400.020.1284459
ATGGGGA28250.019.4790575
TATAGCG1951.0450198E-519.4618345
GTGTAGG16650.019.4345251
GTACTTT60100.019.37806114-15
Produced by FastQC (version 0.11.5)