##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936301_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2189577 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.4567535190587 34.0 31.0 34.0 31.0 34.0 2 32.58646989806707 34.0 31.0 34.0 31.0 34.0 3 32.670060929576806 34.0 31.0 34.0 31.0 34.0 4 36.169790329364986 37.0 35.0 37.0 35.0 37.0 5 36.12539408296671 37.0 35.0 37.0 35.0 37.0 6 36.16657600988684 37.0 36.0 37.0 35.0 37.0 7 36.11162475674525 37.0 36.0 37.0 35.0 37.0 8 36.09420678057908 37.0 36.0 37.0 35.0 37.0 9 37.85378454377261 39.0 38.0 39.0 35.0 39.0 10-11 37.78284664115489 39.0 38.0 39.0 35.0 39.0 12-13 37.72591760874361 39.0 38.0 39.0 35.0 39.0 14-15 39.06009288552081 40.0 38.0 41.0 36.0 41.0 16-17 38.983679496085315 40.0 38.0 41.0 35.0 41.0 18-19 39.01090279081302 40.0 38.5 41.0 35.0 41.0 20-21 38.96708131296593 40.0 38.0 41.0 35.0 41.0 22-23 38.84640229596858 40.0 38.0 41.0 35.0 41.0 24-25 38.994205730147875 40.0 38.0 41.0 35.0 41.0 26-27 38.9108113119566 40.0 38.0 41.0 35.0 41.0 28-29 38.81863369043427 40.0 38.0 41.0 35.0 41.0 30-31 38.69983060655095 40.0 38.0 41.0 35.0 41.0 32-33 38.54718194427508 40.0 38.0 41.0 34.0 41.0 34-35 38.40654267924809 40.0 38.0 41.0 34.0 41.0 36-37 38.25507004320926 40.0 38.0 41.0 34.0 41.0 38-39 38.11955825257573 40.0 38.0 41.0 33.0 41.0 40-41 37.964689755144484 40.0 37.5 41.0 33.0 41.0 42-43 37.97928755188788 40.0 38.0 41.0 33.0 41.0 44-45 37.83452739958449 40.0 37.0 41.0 33.0 41.0 46-47 37.60596635788556 40.0 37.0 41.0 33.0 41.0 48-49 37.355848869439164 40.0 37.0 41.0 32.5 41.0 50-51 37.13876515874984 40.0 36.0 41.0 32.0 41.0 52-53 36.9136394837907 40.0 36.0 41.0 31.0 41.0 54-55 36.708317405599345 39.0 35.5 41.0 31.0 41.0 56-57 36.45009835233016 39.0 35.0 41.0 31.0 41.0 58-59 36.17713375688547 39.0 35.0 41.0 30.5 41.0 60-61 35.883773212816905 38.0 35.0 40.0 30.0 41.0 62-63 35.58177081692035 37.5 35.0 40.0 29.0 41.0 64-65 35.20022885698927 37.0 34.5 40.0 29.0 41.0 66-67 34.84977395177242 36.5 34.0 39.5 29.0 41.0 68-69 34.49581403166 36.0 34.0 39.0 28.0 41.0 70-71 34.12830332068705 35.5 34.0 39.0 28.0 40.0 72-73 33.72821188750156 35.0 34.0 37.5 27.5 40.0 74-75 33.30675719556791 35.0 33.0 37.0 26.5 39.0 76-77 32.590321783613916 35.0 32.5 36.0 26.0 39.0 78-79 32.65752129292552 35.0 33.0 36.0 26.0 38.5 80-81 32.502359359821554 35.0 33.0 36.0 26.0 37.0 82-83 32.240903836677134 35.0 33.0 35.0 26.0 37.0 84-85 31.966260606500708 35.0 33.0 35.0 25.5 36.5 86-87 31.56556951411163 35.0 32.0 35.0 25.0 36.0 88-89 31.09125118687308 34.5 32.0 35.0 23.5 36.0 90-91 30.887506810676214 34.0 32.0 35.0 22.0 35.5 92-93 30.60389152790699 34.0 31.0 35.0 20.0 35.0 94-95 30.454335015393383 34.0 31.0 35.0 19.0 35.0 96-97 30.20235552346412 34.0 31.0 35.0 17.0 35.0 98-99 29.750681981040174 34.0 31.0 35.0 2.0 35.0 100-101 26.349406072497107 30.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20672350802251316 1101 2 0.15347692522788492 1101 3 0.24023566722756584 1101 4 0.07763052964926942 1101 5 0.09658758541117862 1101 6 0.15234231319181646 1101 7 0.15596217071115603 1101 8 0.17448213801504409 1101 9 0.22586577600364421 1101 10-11 0.11535442074654867 1101 12-13 0.2851739040685075 1101 14-15 0.41325625771950314 1101 16-17 0.37617826257993414 1101 18-19 0.27949585954723943 1101 20-21 0.35131126552303726 1101 22-23 0.3255230435459282 1101 24-25 0.25165083096290175 1101 26-27 0.25429523943540744 1101 28-29 0.36664833833387434 1101 30-31 0.31510821450955007 1101 32-33 0.3298724432400064 1101 34-35 0.41684323424735226 1101 36-37 0.303647964257614 1101 38-39 0.3371513790257268 1101 40-41 0.3808586264669245 1101 42-43 0.303277421025264 1101 44-45 0.39847666799563086 1101 46-47 0.44745562498871294 1101 48-49 0.5358681212234657 1101 50-51 0.3988187363001856 1101 52-53 0.4843168839413394 1101 54-55 0.45387847716370544 1101 56-57 0.5343062548256299 1101 58-59 0.33969947523988253 1101 60-61 0.4050551159635205 1101 62-63 0.390382401783107 1101 64-65 0.42664507427079457 1101 66-67 0.34714762074064964 1101 68-69 0.5892146603815078 1101 70-71 0.5921416914246436 1101 72-73 0.5336391479419405 1101 74-75 0.5260188029617368 1101 76-77 0.3554774294210006 1101 78-79 0.3413354649765026 1101 80-81 0.39067844025746723 1101 82-83 0.3953766770206997 1101 84-85 0.5212142155178974 1101 86-87 0.4742851618583508 1101 88-89 0.4081286743450505 1101 90-91 0.5791126325114639 1101 92-93 0.7751825804245556 1101 94-95 0.6777859912378261 1101 96-97 0.6318458365101094 1101 98-99 0.7086938711996318 1101 100-101 1.2144900754354957 1102 1 0.026257649333928157 1102 2 0.177107176379117 1102 3 0.1500574035328981 1102 4 0.11620329005269525 1102 5 0.15778825213435255 1102 6 0.08956665423766452 1102 7 0.09049532323820841 1102 8 0.11149007654090326 1102 9 0.07812161689411568 1102 10-11 0.10380104051657923 1102 12-13 0.22377816431654907 1102 14-15 0.15675570682535778 1102 16-17 0.2803688719536126 1102 18-19 0.20227603865846078 1102 20-21 0.22566883819869332 1102 22-23 0.2711709798117141 1102 24-25 0.2370623065116746 1102 26-27 0.24744409385809973 1102 28-29 0.24066873554266976 1102 30-31 0.2822085337456457 1102 32-33 0.27126945428614135 1102 34-35 0.24845947291164805 1102 36-37 0.1341121278873061 1102 38-39 0.20993636080384448 1102 40-41 0.1673235811241014 1102 42-43 0.2107074292180542 1102 44-45 0.3237052184377518 1102 46-47 0.24402313070954307 1102 48-49 0.29039717735816595 1102 50-51 0.18645610669880597 1102 52-53 0.2338563929392805 1102 54-55 0.21232707450246124 1102 56-57 0.35316322010517354 1102 58-59 0.15738204720925353 1102 60-61 0.31800098708254154 1102 62-63 0.31761677192746873 1102 64-65 0.24259430725663833 1102 66-67 0.4297547625888214 1102 68-69 0.3081960712355354 1102 70-71 0.2770406941649881 1102 72-73 0.17941152978220742 1102 74-75 0.1927902807705948 1102 76-77 0.14012358587493878 1102 78-79 0.1789464207072129 1102 80-81 0.18620602734111458 1102 82-83 0.24355660752328845 1102 84-85 0.31324291851532493 1102 86-87 0.3494406835127286 1102 88-89 0.3645492749609218 1102 90-91 0.4924657415320013 1102 92-93 0.2494780009404174 1102 94-95 0.16268442895853852 1102 96-97 0.15919434062062976 1102 98-99 0.3664284249753784 1102 100-101 0.8738532983074876 1103 1 -0.05284863736542178 1103 2 0.11247538194933071 1103 3 0.14941957559560848 1103 4 0.0689889389241074 1103 5 0.08100755935839743 1103 6 0.022768722868811153 1103 7 0.16489439666383987 1103 8 0.15130961002148524 1103 9 0.1700140911709056 1103 10-11 0.2665815716807245 1103 12-13 0.2304998687824238 1103 14-15 0.27859215567522 1103 16-17 0.29595552281823956 1103 18-19 0.22636427870586573 1103 20-21 0.2470196435641938 1103 22-23 0.28438813221165304 1103 24-25 0.27103095382084064 1103 26-27 0.2780986216822683 1103 28-29 0.3648243138435987 1103 30-31 0.3853680446005683 1103 32-33 0.2876450401289432 1103 34-35 0.21402915453638371 1103 36-37 0.3854448866008724 1103 38-39 0.36554806149877805 1103 40-41 0.3620792779403459 1103 42-43 0.3501272428257707 1103 44-45 0.38696896890912313 1103 46-47 0.35871903115666726 1103 48-49 0.23043192389697964 1103 50-51 0.2512489742309967 1103 52-53 0.19937651360942965 1103 54-55 0.26896689565206344 1103 56-57 0.33247313768315223 1103 58-59 0.33155701892718525 1103 60-61 0.2952780974634024 1103 62-63 0.36486748571295635 1103 64-65 0.36459802432823807 1103 66-67 0.2467129590010444 1103 68-69 0.19584812101197002 1103 70-71 0.18792632687522115 1103 72-73 0.3872041818319829 1103 74-75 0.4065035814224274 1103 76-77 0.2844978788232808 1103 78-79 0.23653220964219202 1103 80-81 0.3917810960261363 1103 82-83 0.5749294998359531 1103 84-85 0.6217323868124645 1103 86-87 0.41137733168381985 1103 88-89 0.2844339918709018 1103 90-91 0.4341190303323046 1103 92-93 0.7511525686288323 1103 94-95 0.6844589266151679 1103 96-97 0.7248574877412466 1103 98-99 0.5569649761793833 1103 100-101 0.7889306026225249 1104 1 -0.01223408230167422 1104 2 -0.0043080223044213994 1104 3 0.004628069048564498 1104 4 0.024274184870463955 1104 5 0.08288361483634077 1104 6 -0.010749243142896603 1104 7 0.062315712242494214 1104 8 0.10661295549636662 1104 9 0.08858588622968 1104 10-11 0.11266383692328219 1104 12-13 0.20560462308263538 1104 14-15 0.2389119559823385 1104 16-17 0.280034495520475 1104 18-19 0.3164671417365028 1104 20-21 0.26173954417942724 1104 22-23 0.1903163573508948 1104 24-25 0.15154691629343375 1104 26-27 0.27101554169860975 1104 28-29 0.3720885202684556 1104 30-31 0.18384736943045255 1104 32-33 0.12366606601022312 1104 34-35 0.2526964364121511 1104 36-37 0.27703802283996026 1104 38-39 0.3297213563526853 1104 40-41 0.4354451921737663 1104 42-43 0.3158599825645112 1104 44-45 0.3448480871420614 1104 46-47 0.42615105431147526 1104 48-49 0.37954161071218806 1104 50-51 0.559449713216388 1104 52-53 0.49869628611146766 1104 54-55 0.3886742918538957 1104 56-57 0.4339541775089302 1104 58-59 0.43389962254174463 1104 60-61 0.3309912215102315 1104 62-63 0.2574787860093224 1104 64-65 0.15666172207765072 1104 66-67 0.23309542459751498 1104 68-69 0.38483841389382434 1104 70-71 0.3878613259597543 1104 72-73 0.34438266469278034 1104 74-75 0.34896740990814834 1104 76-77 0.21492355350287085 1104 78-79 0.30691170166974047 1104 80-81 0.39398530382550945 1104 82-83 0.47678585568213094 1104 84-85 0.5962858924288348 1104 86-87 0.6207520218277587 1104 88-89 0.559285037997423 1104 90-91 0.5769050304256851 1104 92-93 0.6210716506207419 1104 94-95 0.5255981843876043 1104 96-97 0.5264760315213834 1104 98-99 0.38044525844808774 1104 100-101 0.4996872835782007 1105 1 -0.0416817897592594 1105 2 0.019328452521946815 1105 3 -0.005835311064515736 1105 4 -0.0043640028002656095 1105 5 -0.06776972774963497 1105 6 0.0036324304071087 1105 7 0.025383833695336477 1105 8 0.0040019173157404 1105 9 0.031455659786992385 1105 10-11 0.05770796404380718 1105 12-13 0.08518389046204078 1105 14-15 0.09347009137115236 1105 16-17 -0.01471414651268077 1105 18-19 0.1314070056107326 1105 20-21 0.022930559235355474 1105 22-23 -0.04535288799734616 1105 24-25 0.03741460282373765 1105 26-27 0.07286743481919444 1105 28-29 -0.13081761430565564 1105 30-31 0.011927548167449231 1105 32-33 0.013066591213394929 1105 34-35 -0.0726336035611368 1105 36-37 0.02296477010964537 1105 38-39 0.005933912823429921 1105 40-41 0.07563074944935266 1105 42-43 0.07325332885086056 1105 44-45 0.11038793888903342 1105 46-47 -0.006032747104306679 1105 48-49 0.06571807566054844 1105 50-51 -0.09071115422397469 1105 52-53 -0.05000822310031339 1105 54-55 0.039243417432594185 1105 56-57 0.06810707620649481 1105 58-59 0.059886969778567334 1105 60-61 0.08930719750242133 1105 62-63 0.15107720511357314 1105 64-65 0.08930470053001471 1105 66-67 -0.025763038486154244 1105 68-69 -0.022685999968764747 1105 70-71 0.005628315898796643 1105 72-73 6.309965482174107E-4 1105 74-75 0.01925614208717974 1105 76-77 -0.007506130478638795 1105 78-79 0.017379979325703232 1105 80-81 -0.03638031718551815 1105 82-83 -0.0032676700564095995 1105 84-85 -0.03212560897124206 1105 86-87 0.045138624016157536 1105 88-89 0.17757093426590842 1105 90-91 0.2968066494134334 1105 92-93 0.28467277503439803 1105 94-95 0.0509900007748314 1105 96-97 -0.054799858730124384 1105 98-99 -0.11562362121695102 1105 100-101 0.04550781376701707 1106 1 -0.06991104296172779 1106 2 -5.1286649664916695E-6 1106 3 -0.04512177334884626 1106 4 -0.0664152483647058 1106 5 -0.028052862048532745 1106 6 -0.07333512449062596 1106 7 0.028354068217602446 1106 8 0.057157490864625515 1106 9 -0.04695793033868512 1106 10-11 0.0679168925848117 1106 12-13 0.05458641636391093 1106 14-15 0.06544852410145552 1106 16-17 -0.011285449678020143 1106 18-19 0.09263387080076058 1106 20-21 0.1385813101615483 1106 22-23 -0.013154303167787873 1106 24-25 0.07880347807288501 1106 26-27 0.0498894540375332 1106 28-29 0.0073684382185916775 1106 30-31 0.048843033771731825 1106 32-33 0.05128405967970906 1106 34-35 0.23068731705123469 1106 36-37 0.13785893295746376 1106 38-39 0.20143602175006237 1106 40-41 0.05694853548224188 1106 42-43 0.12643170025243222 1106 44-45 0.13298512037297172 1106 46-47 0.13286304516366698 1106 48-49 0.1472750136734362 1106 50-51 0.13344635238239988 1106 52-53 0.10939235872093178 1106 54-55 0.2590322542009673 1106 56-57 0.23256752869627206 1106 58-59 0.2586454137524683 1106 60-61 0.210080951612305 1106 62-63 0.04813187286116971 1106 64-65 0.13603153014538094 1106 66-67 0.1851569325330118 1106 68-69 0.287591773076862 1106 70-71 0.07821642834682763 1106 72-73 -0.09407003090841926 1106 74-75 0.061144565888739066 1106 76-77 0.1542250933602034 1106 78-79 -0.06374279738260924 1106 80-81 -0.07117334775668382 1106 82-83 0.07279999597459863 1106 84-85 0.26368003707981913 1106 86-87 0.1743668741131117 1106 88-89 0.16038360806821927 1106 90-91 0.23964742219550317 1106 92-93 0.40880432716231496 1106 94-95 0.3628513477421187 1106 96-97 0.404937129207557 1106 98-99 0.3836841758490941 1106 100-101 0.6664624475433136 1107 1 0.0019365663750861017 1107 2 0.008930947435878522 1107 3 0.06805580445926296 1107 4 0.038827976441268675 1107 5 0.06943226525778101 1107 6 0.03539141782290045 1107 7 0.038668519455569594 1107 8 0.04232710888079083 1107 9 0.027009157904842596 1107 10-11 0.0958626048517246 1107 12-13 0.014910887428676745 1107 14-15 0.12445855483065316 1107 16-17 0.15822432121058938 1107 18-19 0.11130942621326056 1107 20-21 0.07168066582957522 1107 22-23 -0.05008567150752441 1107 24-25 0.04620101236483265 1107 26-27 0.12132212313768775 1107 28-29 0.05764097231585197 1107 30-31 -0.10153743410745619 1107 32-33 0.0465006307080742 1107 34-35 -0.014311335723348861 1107 36-37 -0.09021088498798946 1107 38-39 0.05938330188624974 1107 40-41 0.017686129716636856 1107 42-43 -0.2129064772085698 1107 44-45 -0.17388362664509316 1107 46-47 -0.13347680036486764 1107 48-49 -0.2527032334383321 1107 50-51 -0.10050868611087793 1107 52-53 -0.018751751308194287 1107 54-55 0.04305644507399364 1107 56-57 -0.056294473368957654 1107 58-59 -0.08355230806079561 1107 60-61 -0.07604480707808392 1107 62-63 0.0276593679408208 1107 64-65 -0.12244015553732623 1107 66-67 -0.279225827965611 1107 68-69 -0.05150603237341045 1107 70-71 0.07692462347490192 1107 72-73 0.13855478472417104 1107 74-75 0.01923482324253456 1107 76-77 -0.08380627544678276 1107 78-79 -0.10937882535713328 1107 80-81 0.08743648693693729 1107 82-83 0.12538851179942867 1107 84-85 -0.07480593594140927 1107 86-87 -4.34974726641002E-4 1107 88-89 0.2344161107250322 1107 90-91 -0.01316630610936187 1107 92-93 0.08912110424608954 1107 94-95 0.23340621771539816 1107 96-97 -0.07589333272722598 1107 98-99 -0.17318145619691094 1107 100-101 0.2136525869954191 1108 1 -0.14328207075280375 1108 2 -0.06297955730904192 1108 3 -0.04691973031295049 1108 4 -0.0013287422753194278 1108 5 -0.08193774825989664 1108 6 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-0.8943967019995327 2316 74-75 -0.8340890027038839 2316 76-77 -0.8186840826005692 2316 78-79 -1.033656899044182 2316 80-81 -1.0932406181702703 2316 82-83 -1.0692572009901475 2316 84-85 -1.0294095027626362 2316 86-87 -1.1207938396485488 2316 88-89 -0.9399087305594769 2316 90-91 -0.9406089716512405 2316 92-93 -0.9172500348107526 2316 94-95 -0.955834263419927 2316 96-97 -0.9323139118156689 2316 98-99 -1.0051608496739668 2316 100-101 -1.0381184612734096 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 19.0 5 45.0 6 72.0 7 189.0 8 393.0 9 724.0 10 1228.0 11 1940.0 12 2925.0 13 4214.0 14 5368.0 15 7111.0 16 9537.0 17 13024.0 18 12054.0 19 7627.0 20 7028.0 21 7601.0 22 7917.0 23 8849.0 24 10673.0 25 13005.0 26 16056.0 27 20244.0 28 25641.0 29 31903.0 30 39105.0 31 49537.0 32 62387.0 33 80507.0 34 113463.0 35 171644.0 36 296747.0 37 526424.0 38 546468.0 39 87864.0 40 37.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.78593206352753 18.07536224451012 13.261340833595117 23.87736485836723 2 16.233365796258298 19.335920013301884 39.147912171038804 25.282802019401014 3 19.7369172219109 23.73152439946163 28.334605268506202 28.19695311012127 4 13.149343457663285 15.112827728826161 35.26795358190189 36.46987523160866 5 14.469050414760476 37.199422536864425 33.009663510349256 15.321863538025838 6 34.74483884330169 34.354717829060135 16.374852311656543 14.525591015981625 7 31.155013045898823 31.438903495972053 19.314598207781685 18.091485250347443 8 28.011346483818567 34.76461435245255 18.455345484538793 18.76869367919009 9 27.71466817563392 15.765556543569831 19.109261743249952 37.410513537546294 10-11 26.520023091270804 25.59771955432386 26.0622167254605 21.820040628944835 12-13 26.723068797156706 22.54047532979581 27.292517336130835 23.443938536916647 14-15 23.49351039036307 24.179898674492836 24.97292856108737 27.353662374056725 16-17 23.70435815573118 27.671019973633992 25.3443668972075 23.28025497342733 18-19 23.488737779032206 25.95540143141803 27.506454443027124 23.049406346522638 20-21 24.07405546738522 25.382574243860113 27.42687507849697 23.1164952102577 22-23 23.793168175025666 25.259662144634394 27.490071137060323 23.45709854327961 24-25 23.638188030838744 25.5765365764208 27.54818011357292 23.237095279167544 26-27 23.65119502137796 25.83363533995898 27.489271057626063 23.025898581036998 28-29 23.13093193187387 25.753758688520396 27.807604108101884 23.307705271503846 30-31 23.474267388246837 25.489068170052565 27.63551721783484 23.401147223865763 32-33 23.388095291378956 25.66775532909469 27.71508314314853 23.229066236377825 34-35 23.7022515080274 25.6798882866548 27.33528732731759 23.282572878000213 36-37 24.02402381829915 25.603461307823384 27.14174929678198 23.230765577095486 38-39 23.599641665374556 25.60073913938034 27.319726878316715 23.47989231692839 40-41 23.560153829606833 25.524904370296298 27.60750934374879 23.307432456348074 42-43 23.84287350016922 25.611430387892742 27.02979268754422 23.515903424393823 44-45 23.42719224368027 25.728296792132603 27.139377535976383 23.705133428210743 46-47 23.65879605331974 26.055882222627226 26.75097280707885 23.53434891697419 48-49 24.133135702768502 25.85012355716746 26.5333878416093 23.483352898454736 50-51 23.74178956344525 25.93984121046533 26.496993065346874 23.821376160742545 52-53 23.989640169213917 26.002983441261556 26.418504826396788 23.588871563127736 54-55 23.751433499986984 25.736091681886542 26.418051755343857 24.094423062782617 56-57 23.629189754045203 26.234341230998016 26.383164619631444 23.75330439532534 58-59 24.016126619769608 25.788867334460658 26.428565719629283 23.766440326140447 60-61 23.96397067067906 25.75406493436207 26.779001799228563 23.50296259573031 62-63 23.82891764025654 25.89573237205177 26.68563380050119 23.589716187190493 64-65 23.61282758834872 25.866360486709215 26.678828916468223 23.84198300847384 66-67 23.699554754183115 26.075104917525167 26.33323696768828 23.89210336060344 68-69 23.17141621418201 26.012627096466577 26.8013182454876 24.014638443863813 70-71 23.505661493282364 25.861852028192718 26.714301536030433 23.918184942494484 72-73 23.684000797426496 26.116014593909643 26.3147318151021 23.88525279356176 74-75 23.45763588126839 26.27621682178795 26.26954886720129 23.996598429742367 76-77 23.60154624255965 26.24093515871409 26.214142468016366 23.943376130709893 78-79 23.644694778157113 26.043219902139736 26.372872522204858 23.93921279749829 80-81 23.480051782175977 26.69435568344548 26.20845819689969 23.617134337478845 82-83 23.651919982718123 26.440746317667752 26.120456142898835 23.78687755671529 84-85 23.773352241198594 25.994957902597093 25.912019054013015 24.319670802191304 86-87 23.485881519079953 25.875140041612365 26.46352058897501 24.17545785033267 88-89 23.600360259292973 26.19379533532666 26.147051669093436 24.058792736286936 90-91 23.714680941079564 26.237617298799933 26.2870026504541 23.7606991096664 92-93 23.627276764355678 25.99365280616095 26.192263129363063 24.186807300120307 94-95 23.553103381500424 25.990360492726523 26.289556987318292 24.166979138454764 96-97 23.566153751998733 25.97301317690281 26.15309888545992 24.30773418563854 98-99 23.49613406122931 25.84031473751854 26.08518721337726 24.578363987874894 100-101 23.343229567124073 26.055060997596087 25.909049493424114 24.692659941855723 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 208.0 1 146.5 2 179.5 3 293.5 4 238.5 5 208.0 6 447.5 7 949.5 8 1493.5 9 1717.5 10 1453.0 11 1212.0 12 1250.5 13 1393.0 14 1565.0 15 1660.0 16 1797.5 17 1964.5 18 2071.0 19 2213.0 20 2438.5 21 2682.5 22 3088.5 23 3661.0 24 4404.5 25 5430.0 26 6665.5 27 8133.5 28 9791.0 29 12171.5 30 15697.5 31 19651.5 32 23527.5 33 27633.5 34 32010.5 35 35362.5 36 40142.0 37 45739.0 38 49385.0 39 53046.5 40 55537.0 41 60328.0 42 65502.5 43 69465.5 44 73931.0 45 77274.5 46 78378.0 47 77590.5 48 81525.5 49 82071.0 50 79317.0 51 78733.0 52 80063.0 53 84014.0 54 86751.5 55 83681.0 56 76378.5 57 70460.0 58 67076.0 59 61977.5 60 54417.5 61 46606.5 62 39546.0 63 32940.0 64 26512.5 65 20885.5 66 14791.5 67 11876.0 68 8434.0 69 4558.0 70 3385.0 71 2827.0 72 2438.5 73 2239.0 74 1806.5 75 1410.5 76 945.0 77 439.0 78 224.0 79 100.5 80 56.0 81 34.0 82 15.5 83 5.0 84 4.0 85 2.5 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26630714517004883 2 0.018862090714325187 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.2835460913226617E-4 12-13 1.1417730456613308E-4 14-15 0.0 16-17 7.078992883100252E-4 18-19 0.0 20-21 9.134184365290646E-5 22-23 4.567092182645323E-5 24-25 0.0026717489268475144 26-27 0.0025347361613681546 28-29 0.002283546091322662 30-31 0.0013929631157068238 32-33 0.0018268368730581293 34-35 0.002032356021277169 36-37 0.0 38-39 1.5984822639258632E-4 40-41 5.252156010042123E-4 42-43 0.0036536737461162587 44-45 0.0038820283552485255 46-47 0.01342725101697725 48-49 0.014020973000721144 50-51 0.0063025872120505465 52-53 0.007261676570406064 54-55 2.7402553095871943E-4 56-57 0.0031512936060252733 58-59 0.0013472921938803705 60-61 0.001255950350227464 62-63 0.0 64-65 6.850638273967985E-4 66-67 0.0 68-69 0.0 70-71 2.0551914821903956E-4 72-73 0.0015299758811861835 74-75 0.0 76-77 0.19544870995630662 78-79 0.10387851169426789 80-81 9.362538974422914E-4 82-83 0.0 84-85 5.480510619174389E-4 86-87 0.12454460382073798 88-89 0.5851130149796057 90-91 0.3079818613366874 92-93 0.2926364316029991 94-95 0.0021008624040168493 96-97 0.002922938996893007 98-99 0.0031969645278517264 100-101 0.001073266662921651 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2189577.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.17848947275409 #Duplication Level Percentage of deduplicated Percentage of total 1 63.586957065570225 22.368930997356287 2 15.330075862069581 10.785778246606728 3 6.838921706267842 7.21748805746798 4 3.860552258327453 5.432335879143573 5 2.4191448845451418 4.2550931427019085 6 1.6087844308279424 3.3956763698286876 7 1.1432524756425582 2.815252662536425 8 0.8206460051033747 2.3095269481109413 9 0.6349415979700508 2.010265768800278 >10 3.3652829508867685 21.346446732802733 >50 0.24214789816177432 5.90554555395804 >100 0.14084919316957656 8.877655369475534 >500 0.005715716063660104 1.3022954987874376 >1k 0.0025980527562091374 1.714535224191377 >5k 1.299026378104569E-4 0.2631735482320462 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5759 0.2630188387985442 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4381 0.20008430852169165 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 3299 0.15066837110546924 No Hit CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG 3169 0.14473115126803032 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2862 0.13071017826730916 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.567092182645324E-5 0.0 5 0.0 0.0 0.0 4.567092182645324E-5 0.0 6 0.0 0.0 0.0 4.567092182645324E-5 0.0 7 4.567092182645324E-5 0.0 0.0 4.567092182645324E-5 0.0 8 4.567092182645324E-5 0.0 0.0 4.567092182645324E-5 0.0 9 4.567092182645324E-5 0.0 0.0 4.567092182645324E-5 0.0 10-11 4.567092182645324E-5 0.0 0.0 4.567092182645324E-5 0.0 12-13 6.850638273967986E-5 0.0 0.0 4.567092182645324E-5 4.567092182645324E-5 14-15 9.134184365290648E-5 0.0 0.0 6.850638273967986E-5 4.567092182645324E-5 16-17 9.134184365290648E-5 0.0 0.0 2.0551914821903956E-4 4.567092182645324E-5 18-19 1.5984822639258635E-4 0.0 0.0 2.5119007004549283E-4 4.567092182645324E-5 20-21 1.8268368730581296E-4 0.0 0.0 5.480510619174388E-4 4.567092182645324E-5 22-23 2.283546091322662E-4 0.0 0.0 0.0011646085065745576 4.567092182645324E-5 24-25 2.7402553095871943E-4 0.0 0.0 0.0016898241075787696 4.567092182645324E-5 26-27 2.7402553095871943E-4 0.0 0.0 0.0018953432557978094 4.567092182645324E-5 28-29 3.653673746116259E-4 0.0 0.0 0.002443394317715248 4.567092182645324E-5 30-31 4.110382964380791E-4 0.0 0.0 0.005092307783649536 4.567092182645324E-5 32-33 4.110382964380791E-4 0.0 0.0 0.010161780106385845 4.567092182645324E-5 34-35 4.338737573513058E-4 0.0 0.0 0.0158706453346925 4.567092182645324E-5 36-37 5.023801400909857E-4 0.0 0.0 0.02498199423906992 4.567092182645324E-5 38-39 5.480510619174389E-4 0.0 0.0 0.04235977999403538 4.567092182645324E-5 40-41 5.480510619174389E-4 0.0 0.0 0.06197544091849704 4.567092182645324E-5 42-43 5.480510619174389E-4 0.0 0.0 0.08083753163282223 4.567092182645324E-5 44-45 5.480510619174389E-4 0.0 0.0 0.10467775282623082 4.567092182645324E-5 46-47 6.393929055703454E-4 0.0 0.0 0.13395281371698736 4.567092182645324E-5 48-49 7.76405671049705E-4 0.0 0.0 0.16660752282290142 4.567092182645324E-5 50-51 7.76405671049705E-4 0.0 0.0 0.20207099362114234 4.567092182645324E-5 52-53 7.76405671049705E-4 0.0 0.0 0.23965816228431336 4.567092182645324E-5 54-55 7.76405671049705E-4 0.0 0.0 0.2772453309474844 4.567092182645324E-5 56-57 8.220765928761583E-4 0.0 0.0 0.32579352084900415 4.567092182645324E-5 58-59 8.220765928761583E-4 0.0 0.0 0.37804105541846666 4.567092182645324E-5 60-61 8.220765928761583E-4 0.0 0.0 0.43807548215933945 4.567092182645324E-5 62-63 8.220765928761583E-4 0.0 0.0 0.5014667216544565 4.567092182645324E-5 64-65 8.677475147026115E-4 0.0 0.0 0.5653375058287514 4.567092182645324E-5 66-67 8.677475147026115E-4 0.0 0.0 0.6395984247185644 4.567092182645324E-5 68-69 8.677475147026115E-4 0.0 0.0 0.7226738315208828 4.567092182645324E-5 70-71 8.677475147026115E-4 0.0 0.0 0.8202269205421869 4.567092182645324E-5 72-73 8.905829756158381E-4 0.0 0.0 0.9174146421888794 4.567092182645324E-5 74-75 9.134184365290648E-4 0.0 0.0 1.0192836333227833 4.567092182645324E-5 76-77 9.134184365290648E-4 0.0 0.0 1.1335522797325694 4.567092182645324E-5 78-79 9.134184365290648E-4 0.0 0.0 1.2664546622475483 4.567092182645324E-5 80-81 0.0012102794284010107 0.0 0.0 1.417762426258588 4.567092182645324E-5 82-83 0.0012331148893142375 0.0 0.0 1.575167258333459 4.567092182645324E-5 84-85 0.0013016212720539174 0.0 0.0 1.734992649265132 4.567092182645324E-5 86-87 0.001370127654793597 0.0 0.0 1.90872483589296 4.567092182645324E-5 88-89 0.001370127654793597 0.0 0.0 2.1029632664208657 4.567092182645324E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5645 0.0 32.87607 1 GTATCAA 8670 0.0 32.54729 1 GTACATG 11110 0.0 28.868555 1 TACATGG 11430 0.0 27.35564 2 ACATGGG 11245 0.0 26.914701 3 ATCAACG 10575 0.0 26.466713 3 TCAACGC 10755 0.0 26.067862 4 TATCAAC 10940 0.0 25.718468 2 CAACGCA 11040 0.0 25.394918 5 AACGCAG 11160 0.0 25.164358 6 CATGGGG 5465 0.0 23.871016 4 ACGCAGA 12690 0.0 22.16774 7 CGCAGAG 12865 0.0 21.755577 8 GAGTACT 5540 0.0 21.364323 12-13 CATGGGA 6030 0.0 21.083652 4 GCAGAGT 13740 0.0 20.128445 9 ATGGGGA 2825 0.0 19.479057 5 TATAGCG 195 1.0450198E-5 19.461834 5 GTGTAGG 1665 0.0 19.434525 1 GTACTTT 6010 0.0 19.378061 14-15 >>END_MODULE