FastQCFastQC Report
Thu 26 May 2016
SRR936300_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936300_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2190806
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32010.14611060951996663No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22140.1010586971187773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23950.046.4579471
GTATCAA42700.040.5836261
ATCAACG51750.032.8354453
TCAACGC52800.032.0913544
CAACGCA53700.031.4653765
TATCAAC56400.030.2961142
AACGCAG56150.030.0896936
GTACATG75600.029.874061
ACATGGG73300.029.1226833
TACATGG76350.028.3312662
ACGCAGA67000.025.0061977
CGCAGAG67750.024.7305018
CATGGGG48250.022.8558624
GCAGAGT77250.021.260339
GTACCTG19850.021.2288841
TACCTGG20000.020.8262712
GTATAGG7200.020.3857571
GAGTACA59200.020.1547051
AGTACAT51750.019.8476092
GTCCAAG15400.019.676911
Produced by FastQC (version 0.11.5)