##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936300_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2190806 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.725274624955382 33.0 31.0 34.0 30.0 34.0 2 31.82010912878639 34.0 31.0 34.0 30.0 34.0 3 31.891075704558048 34.0 31.0 34.0 30.0 34.0 4 35.37348628769503 37.0 35.0 37.0 33.0 37.0 5 35.35194809581496 37.0 35.0 37.0 33.0 37.0 6 35.43810953594248 37.0 35.0 37.0 33.0 37.0 7 35.38054715935596 37.0 35.0 37.0 33.0 37.0 8 35.37760121160888 37.0 35.0 37.0 33.0 37.0 9 37.04350636249855 39.0 37.0 39.0 33.0 39.0 10-11 36.96129780546521 39.0 37.0 39.0 33.0 39.0 12-13 36.86844978514756 39.0 37.0 39.0 33.0 39.0 14-15 38.14006032483022 40.0 38.0 41.0 33.0 41.0 16-17 38.08398986491729 40.0 38.0 41.0 33.0 41.0 18-19 38.0148673593189 40.0 38.0 41.0 33.0 41.0 20-21 37.929522513631966 40.0 38.0 41.0 33.0 41.0 22-23 37.77396446787164 40.0 38.0 41.0 32.5 41.0 24-25 37.9356624913388 40.0 38.0 41.0 33.0 41.0 26-27 37.89392625362538 40.0 38.0 41.0 33.0 41.0 28-29 37.76042926667172 40.0 38.0 41.0 32.5 41.0 30-31 37.621319733467956 40.0 38.0 41.0 32.0 41.0 32-33 37.46152192389468 40.0 37.5 41.0 32.0 41.0 34-35 37.21006789282118 40.0 37.0 41.0 31.0 41.0 36-37 37.01856120532808 40.0 37.0 41.0 31.0 41.0 38-39 36.87022333333029 40.0 37.0 41.0 30.0 41.0 40-41 36.69643158727884 40.0 36.5 41.0 30.0 41.0 42-43 36.52249309158364 40.0 36.0 41.0 30.0 41.0 44-45 36.35907675074836 40.0 36.0 41.0 30.0 41.0 46-47 36.11404387243782 39.5 35.5 41.0 29.0 41.0 48-49 35.96952537102783 39.0 35.0 41.0 28.5 41.0 50-51 35.67032703945489 39.5 35.0 40.5 28.0 41.0 52-53 35.687896600611836 39.0 35.0 40.5 28.0 41.0 54-55 35.76490433201297 39.0 35.0 41.0 28.0 41.0 56-57 35.59907449587047 39.0 35.0 41.0 28.0 41.0 58-59 35.368365797793146 39.0 35.0 41.0 27.5 41.0 60-61 35.08184453575534 38.0 35.0 40.5 27.0 41.0 62-63 34.7746279223263 38.0 34.5 40.0 26.0 41.0 64-65 34.440033713619556 37.0 34.0 40.0 26.0 41.0 66-67 34.07854962967967 37.0 34.0 40.0 26.0 41.0 68-69 33.704367707592546 36.0 34.0 39.0 25.5 41.0 70-71 33.304816811712215 36.0 33.5 39.0 24.5 41.0 72-73 32.89818998122152 35.0 33.0 38.5 24.0 40.0 74-75 32.47906386964432 35.0 33.0 37.0 22.5 39.5 76-77 32.07621122089313 35.0 32.5 37.0 21.5 39.0 78-79 31.68412196241931 35.0 32.0 36.0 20.0 39.0 80-81 31.29763132837869 35.0 32.0 36.0 19.5 37.5 82-83 30.95642334373742 35.0 31.5 35.5 18.0 37.0 84-85 30.639960589846844 35.0 31.0 35.0 16.0 37.0 86-87 30.32010502070927 34.5 31.0 35.0 9.0 36.0 88-89 30.012714042229206 34.0 31.0 35.0 6.0 36.0 90-91 29.67584509993126 34.0 30.5 35.0 2.0 35.5 92-93 29.375466837319234 34.0 30.0 35.0 2.0 35.0 94-95 29.019632044097012 34.0 30.0 35.0 2.0 35.0 96-97 28.61523019381908 34.0 29.5 35.0 2.0 35.0 98-99 28.13779403561977 34.0 29.0 35.0 2.0 35.0 100-101 24.896589656957303 30.5 23.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3063382012467635 1101 2 0.2415129257660098 1101 3 0.3452713302767698 1101 4 0.17059649221062045 1101 5 0.36787685319915653 1101 6 0.3681875079097523 1101 7 0.40561789186325825 1101 8 0.3513203060449186 1101 9 0.3523572493215781 1101 10-11 0.6807319250499333 1101 12-13 0.5481521099912428 1101 14-15 0.6601357527058767 1101 16-17 0.6961975839447021 1101 18-19 0.6652884984182208 1101 20-21 0.6621655015313834 1101 22-23 0.6904767058475301 1101 24-25 0.5937390702431742 1101 26-27 0.5185351759254786 1101 28-29 0.4865894038539196 1101 30-31 0.5581252181731315 1101 32-33 0.6818625144543446 1101 34-35 0.4920240123788915 1101 36-37 0.4776381648693331 1101 38-39 0.46094432460124324 1101 40-41 0.4191831995101154 1101 42-43 0.3750744634649763 1101 44-45 0.4251506546968429 1101 46-47 0.4485211689011024 1101 48-49 0.40420140927167125 1101 50-51 0.6079605933743224 1101 52-53 0.6824537141451756 1101 54-55 0.5441110781905891 1101 56-57 0.4962177831126624 1101 58-59 0.6003436981545462 1101 60-61 0.4628084859912036 1101 62-63 0.4103180386668228 1101 64-65 0.40269642784146953 1101 66-67 0.5581419528429095 1101 68-69 0.5486256524200357 1101 70-71 0.6775824863707314 1101 72-73 0.6121210366989303 1101 74-75 0.5895377863147928 1101 76-77 0.5079413774030641 1101 78-79 0.5962153110272048 1101 80-81 0.6029790743110226 1101 82-83 0.6721662502025723 1101 84-85 0.5557672301993968 1101 86-87 0.5129711001651884 1101 88-89 0.43025041384939655 1101 90-91 0.5610346224472131 1101 92-93 0.6554076876895394 1101 94-95 0.820533815779914 1101 96-97 0.9022048960965101 1101 98-99 1.061566937155746 1101 100-101 1.0379652282848255 1102 1 0.29235224938555504 1102 2 0.2520491686703821 1102 3 0.187327074752897 1102 4 0.2217480192745711 1102 5 0.25328271870381514 1102 6 0.10846267952764066 1102 7 0.08035525618677752 1102 8 0.05489713730428747 1102 9 0.09543360904299192 1102 10-11 0.22807963855584745 1102 12-13 0.27940934373332027 1102 14-15 0.3534098196728692 1102 16-17 0.26820186040669824 1102 18-19 0.3757662745025172 1102 20-21 0.2947036194827817 1102 22-23 0.33796606119393147 1102 24-25 0.3852913920783081 1102 26-27 0.2868423560452129 1102 28-29 0.16107468901014244 1102 30-31 0.10993748138042747 1102 32-33 -0.004501723840427019 1102 34-35 0.05036979003895681 1102 36-37 0.07254069657147966 1102 38-39 -0.021685532954599296 1102 40-41 -0.0015741318154667283 1102 42-43 -0.038957379019969096 1102 44-45 0.03848120072309058 1102 46-47 0.07516638107652085 1102 48-49 -0.2012988582154236 1102 50-51 -0.28549792447235234 1102 52-53 -0.11795316606141881 1102 54-55 -0.10194187009458489 1102 56-57 -0.04676296420212367 1102 58-59 -0.03305351559331626 1102 60-61 -0.19686742570669225 1102 62-63 -0.0014410658191579273 1102 64-65 -0.14950020057190017 1102 66-67 -0.0479118893794066 1102 68-69 -0.06339771876125866 1102 70-71 0.014759055334508275 1102 72-73 0.014263376476669976 1102 74-75 0.05055671362909919 1102 76-77 -0.10691080509447914 1102 78-79 -0.047816700806084356 1102 80-81 -0.04370032401931212 1102 82-83 0.0027719647928741153 1102 84-85 -0.05312514004715396 1102 86-87 -0.04602514168977834 1102 88-89 -0.1332242587183643 1102 90-91 0.07697335499426927 1102 92-93 0.08127448050683483 1102 94-95 0.01173891799551896 1102 96-97 0.06463396759842865 1102 98-99 0.17564469802645277 1102 100-101 0.1721238155087974 1103 1 0.147398727667845 1103 2 0.12755651338328278 1103 3 0.1903430728661526 1103 4 0.1364160633938809 1103 5 0.0833540034900011 1103 6 0.08349420138180363 1103 7 0.05274925706891054 1103 8 0.12759107259087443 1103 9 0.1472116457646777 1103 10-11 0.21128548767965327 1103 12-13 0.17560708554904636 1103 14-15 0.1660358332206826 1103 16-17 0.32226848850039147 1103 18-19 0.21826713541042153 1103 20-21 0.2434403815127908 1103 22-23 0.22269304459948813 1103 24-25 0.3233323471193046 1103 26-27 0.3121652703782871 1103 28-29 0.2804842270638872 1103 30-31 0.21189261531942805 1103 32-33 0.2881772818794346 1103 34-35 0.2017708538578873 1103 36-37 0.13429264012815167 1103 38-39 0.16242962831857 1103 40-41 0.1608049180108182 1103 42-43 0.052285428168353576 1103 44-45 0.07844545894280941 1103 46-47 0.1878778944639734 1103 48-49 0.32381937224143087 1103 50-51 0.35049570830113197 1103 52-53 0.3868829605890127 1103 54-55 0.40094667067054246 1103 56-57 0.4230386428617834 1103 58-59 0.3141084699369827 1103 60-61 0.18896275544123142 1103 62-63 0.3056164449777796 1103 64-65 0.3856312886725206 1103 66-67 0.4673683529319206 1103 68-69 0.3216326641694991 1103 70-71 0.3107557127017557 1103 72-73 0.28901588895781316 1103 74-75 0.3740344645991982 1103 76-77 0.3105958175839518 1103 78-79 0.39157833746335413 1103 80-81 0.3585604389411756 1103 82-83 0.5553656479583502 1103 84-85 0.5001539117668727 1103 86-87 0.4332237544409452 1103 88-89 0.3434110230872811 1103 90-91 0.5271430384648923 1103 92-93 0.5722440803847775 1103 94-95 0.547890737559964 1103 96-97 0.7487442938194029 1103 98-99 0.8314228271513571 1103 100-101 0.95727612233906 1104 1 0.36419225628857177 1104 2 0.26877800170759514 1104 3 0.33028148738855023 1104 4 0.27737221277867263 1104 5 0.4102126020977437 1104 6 0.46725716182100996 1104 7 0.45912926476064087 1104 8 0.36973195517839486 1104 9 0.4609098975733943 1104 10-11 0.5593997228040237 1104 12-13 0.47324291043567257 1104 14-15 0.6658175996862852 1104 16-17 0.518974723707359 1104 18-19 0.4261955558435844 1104 20-21 0.5085151435982311 1104 22-23 0.5960894604589129 1104 24-25 0.5203328297883871 1104 26-27 0.5263574191332907 1104 28-29 0.6504416902377841 1104 30-31 0.5638974987948586 1104 32-33 0.6018310094011312 1104 34-35 0.6371693397740117 1104 36-37 0.7050707697983682 1104 38-39 0.700129568178518 1104 40-41 0.746215922349684 1104 42-43 0.7124430312388554 1104 44-45 0.7056616714617476 1104 46-47 0.708491477856029 1104 48-49 0.698657213497512 1104 50-51 0.7821967425551506 1104 52-53 0.7879928413671706 1104 54-55 0.8751216808014348 1104 56-57 0.9561476770332078 1104 58-59 0.8606549339342564 1104 60-61 0.8515639030063298 1104 62-63 0.9454229643658536 1104 64-65 0.9520953375692258 1104 66-67 0.9590072080511831 1104 68-69 0.7868150588792417 1104 70-71 0.8204611038831118 1104 72-73 0.8768380447592676 1104 74-75 0.8093861032220175 1104 76-77 0.7494146721353658 1104 78-79 0.9298034057984559 1104 80-81 0.9055197527767476 1104 82-83 0.843336992385332 1104 84-85 0.7737119056186543 1104 86-87 0.75105166613573 1104 88-89 0.8322555597636772 1104 90-91 0.8951769233831577 1104 92-93 0.8494170591757282 1104 94-95 0.4941476001076843 1104 96-97 0.6523391460374874 1104 98-99 0.8806501694514957 1104 100-101 0.8805996216124754 1105 1 0.233353271754428 1105 2 0.20733390111436023 1105 3 0.20673115539214493 1105 4 0.09588580731479368 1105 5 0.20063963550592945 1105 6 0.23322036105236776 1105 7 0.24407379547822217 1105 8 0.2677451903465524 1105 9 0.3014048943521175 1105 10-11 0.2689879578790837 1105 12-13 0.2649454207977584 1105 14-15 0.26000142477649746 1105 16-17 0.3420945589566955 1105 18-19 0.3228577164456823 1105 20-21 0.18809782988304136 1105 22-23 0.3197641747334572 1105 24-25 0.31766196242682554 1105 26-27 0.2953825078580721 1105 28-29 0.3176595119093477 1105 30-31 0.5096042619881302 1105 32-33 0.4526657485521284 1105 34-35 0.2279255765770145 1105 36-37 0.28722265769045663 1105 38-39 0.4106823245121447 1105 40-41 0.39882590197928636 1105 42-43 0.422037969550459 1105 44-45 0.3973574785872884 1105 46-47 0.45436098073655984 1105 48-49 0.3306480375114802 1105 50-51 0.370552848272645 1105 52-53 0.5449286429811622 1105 54-55 0.5905033742624255 1105 56-57 0.6306750057562027 1105 58-59 0.6643260731493044 1105 60-61 0.5326082672602084 1105 62-63 0.42332723469993283 1105 64-65 0.4771661870554951 1105 66-67 0.37063732345857403 1105 68-69 0.3368372168000491 1105 70-71 0.5145451252291906 1105 72-73 0.6340568719163144 1105 74-75 0.5563173242910366 1105 76-77 0.6056574335709044 1105 78-79 0.53259195220161 1105 80-81 0.5291955842573799 1105 82-83 0.36585204673740535 1105 84-85 0.3491181031332431 1105 86-87 0.38992410726828197 1105 88-89 0.37726257954194864 1105 90-91 0.4609933962145689 1105 92-93 0.440308105513715 1105 94-95 0.29934856686335465 1105 96-97 0.29035580730432287 1105 98-99 0.34373097696805743 1105 100-101 0.358257734204507 1106 1 0.06537049485373458 1106 2 0.12046361221824498 1106 3 0.16659027694055695 1106 4 0.16103278021288503 1106 5 0.21331777714473787 1106 6 0.11484879715935392 1106 7 0.1398860884605142 1106 8 0.08908624911636309 1106 9 0.19001243908341792 1106 10-11 0.14779710786825007 1106 12-13 0.09409680297953571 1106 14-15 0.10872589581723702 1106 16-17 0.2003729213499028 1106 18-19 0.13190697795354822 1106 20-21 0.15684287209916903 1106 22-23 0.2016245383881099 1106 24-25 0.16538895153807687 1106 26-27 0.24044977893905184 1106 28-29 0.1408994018997518 1106 30-31 0.21506619201227295 1106 32-33 0.14656515922882107 1106 34-35 0.040022786868313176 1106 36-37 0.08859182830400414 1106 38-39 0.1287005072449361 1106 40-41 0.22149334931553 1106 42-43 0.06890107086918107 1106 44-45 0.14709423235617436 1106 46-47 0.27491314726392346 1106 48-49 0.20106692429794748 1106 50-51 0.23549918812219772 1106 52-53 0.1580882458941133 1106 54-55 0.2624985702907807 1106 56-57 0.3322696225219275 1106 58-59 0.3068918641504226 1106 60-61 0.42298549898847426 1106 62-63 0.4449367635121533 1106 64-65 0.2448556808101401 1106 66-67 0.32803194273946445 1106 68-69 0.3819733648002952 1106 70-71 0.4607676808724577 1106 72-73 0.5284740928874001 1106 74-75 0.4612185652364289 1106 76-77 0.35497434769144576 1106 78-79 0.39134299696386776 1106 80-81 0.5074216021266835 1106 82-83 0.4352512262120847 1106 84-85 0.35415605799177285 1106 86-87 0.3690798909947546 1106 88-89 0.30629661944412945 1106 90-91 0.5417696013671822 1106 92-93 0.4296269834003539 1106 94-95 0.37433843363681873 1106 96-97 0.4190780146935005 1106 98-99 0.4273296121989887 1106 100-101 0.4394835257086527 1107 1 -0.04205592229742905 1107 2 0.16316513232483487 1107 3 0.12104133273836126 1107 4 0.045874864757458056 1107 5 -0.0033173468055238686 1107 6 0.19454437209475373 1107 7 0.027084035408186935 1107 8 0.10682662984956437 1107 9 0.09791325868935985 1107 10-11 0.1830965618007454 1107 12-13 0.06426698224310456 1107 14-15 0.17168692416026232 1107 16-17 0.02442828007890796 1107 18-19 0.14019955809727946 1107 20-21 0.25996547485272004 1107 22-23 0.27193022516136267 1107 24-25 0.355206836510618 1107 26-27 0.24617440345456032 1107 28-29 0.2583063130350851 1107 30-31 0.194670166899229 1107 32-33 0.257211709212676 1107 34-35 0.20986075824266948 1107 36-37 0.16209317076201302 1107 38-39 0.16601326968034158 1107 40-41 0.2477410530057753 1107 42-43 -0.057417667018420104 1107 44-45 0.05871873271954797 1107 46-47 0.2188137952774909 1107 48-49 0.1924692630011151 1107 50-51 0.2413815262197474 1107 52-53 0.14825978780302762 1107 54-55 0.1678146535433669 1107 56-57 0.13977729371055858 1107 58-59 0.34048435244177 1107 60-61 0.40010580038543964 1107 62-63 0.3951791377450391 1107 64-65 0.27610303122195745 1107 66-67 0.21290746256342885 1107 68-69 0.25465131618918946 1107 70-71 0.3764615701650982 1107 72-73 0.3317155485963852 1107 74-75 0.11293373198385126 1107 76-77 0.18577883836489661 1107 78-79 0.19513922596903654 1107 80-81 0.29001393396615427 1107 82-83 0.4219443635263609 1107 84-85 0.46900245757995407 1107 86-87 0.2694192906233077 1107 88-89 0.30115843711208967 1107 90-91 0.40527581402060875 1107 92-93 0.21142090600209684 1107 94-95 0.22631717132254536 1107 96-97 0.3189339275649452 1107 98-99 0.296593127622117 1107 100-101 0.500119351168923 1108 1 -0.07282599689154878 1108 2 -0.11750255818270006 1108 3 -0.019050569167934128 1108 4 0.014859799670432494 1108 5 -0.0400817658977175 1108 6 0.04070374998953952 1108 7 -0.0778523401951432 1108 8 0.01619599263051441 1108 9 0.05811753931926944 1108 10-11 -0.0038013222614665665 1108 12-13 -4.12336171407901E-4 1108 14-15 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-1.6013626528345242 2316 94-95 -1.4969685977542504 2316 96-97 -1.7519062996460626 2316 98-99 -1.5488045616340749 2316 100-101 -1.4256451343728287 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19471.0 3 4434.0 4 3557.0 5 2950.0 6 2748.0 7 3050.0 8 3549.0 9 4274.0 10 4908.0 11 5942.0 12 7373.0 13 10608.0 14 11126.0 15 9019.0 16 9916.0 17 12214.0 18 11478.0 19 9609.0 20 9481.0 21 9911.0 22 10645.0 23 11694.0 24 13294.0 25 15386.0 26 18548.0 27 21725.0 28 26265.0 29 31819.0 30 39421.0 31 48656.0 32 61522.0 33 80406.0 34 113450.0 35 170137.0 36 285238.0 37 486158.0 38 504740.0 39 95900.0 40 184.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.37549565956802 17.500921334265552 12.643354816671607 24.480228189494827 2 16.004459200913754 18.48094088774071 40.022012967655726 25.492586943689805 3 20.165217724750338 22.56268248724986 27.761891458090243 29.51020832990956 4 12.836260206319782 14.627624245838158 35.649710112444694 36.88640543539737 5 14.515160824846744 36.61071124840399 33.701662502687604 15.172465424061668 6 35.41869135073226 33.97662177478955 16.50507995038963 14.099606924088556 7 31.39738601235418 30.6684329403344 19.228128668958806 18.70605237835262 8 27.607711199773576 35.33735357759132 18.43867834089602 18.616256881739083 9 26.91424666176967 16.130880450974384 19.055886511353567 37.89898637590238 10-11 26.4792053663924 25.487944628894592 26.49406332228002 21.53878668243299 12-13 26.56068934711559 22.253182868851354 27.3371452361448 23.848982547888255 14-15 23.21339921770488 24.212826088494708 24.80930500722289 27.764469686577524 16-17 23.878990942901957 27.53666427515024 25.266192213548187 23.318152568399615 18-19 23.563701005393888 26.306684510104727 26.954403137681695 23.17521134681969 20-21 24.027868374288534 25.8059767374027 26.992340898706228 23.173813989602536 22-23 23.660079668471063 25.570853902486014 26.930266806294963 23.83879962274797 24-25 23.57388044727094 25.76647412883728 27.167853982013014 23.49179144187877 26-27 23.6463242503976 26.071633339845924 26.927050142698803 23.354992267057664 28-29 23.338518093091928 26.036913906813115 27.24153736724991 23.38303063284505 30-31 23.588694168468987 25.78368723500174 27.121190159612414 23.506428436916863 32-33 23.42112459307217 25.86561326894009 27.229115375081204 23.484146762906537 34-35 23.849803053522805 25.983731234706696 26.82060215987802 23.345863551892474 36-37 23.901602798769748 25.974402089033877 26.688471767981127 23.435523344215248 38-39 23.569199980097125 25.99861184326532 26.93104318809203 23.501144988545526 40-41 23.70659020057671 25.82968926056762 27.05621684126949 23.407503697586186 42-43 23.788273598454943 25.77784371967975 26.810076544330425 23.623806137534885 44-45 23.60396701781699 26.05299800392243 26.732072629425662 23.61096234883491 46-47 23.56178510146229 26.3083349981662 26.539033812812846 23.590846087558663 48-49 23.99087508989 26.056366620445715 26.558672506792647 23.39408578287164 50-51 23.70956948523179 26.18567003235382 26.449010791717626 23.65574969069677 52-53 23.82953803608564 26.26664232146765 26.28093289212248 23.62288675032423 54-55 23.80255802293808 26.036711202485456 26.437781273121676 23.72294950145479 56-57 23.627113691858924 26.40684023034084 26.41103716905246 23.555008908747773 58-59 23.86902473995481 26.162004551880152 26.45901885975494 23.5099518484101 60-61 23.899561888721937 26.17322732801656 26.575815450210712 23.3513953330508 62-63 23.638955832410876 26.335784119811745 26.489316593592548 23.53594345418483 64-65 23.63108498735416 26.18369419149065 26.533103390856418 23.652117430298784 66-67 23.592595284769136 26.43630824531381 26.277378957725634 23.69371751219142 68-69 23.35097437835844 26.130941295091546 26.66326904145684 23.854815285093174 70-71 23.521606752315055 26.018501170799073 26.614575605688724 23.84531647119715 72-73 23.592496371845893 26.249419770550215 26.397252143408867 23.760831714195028 74-75 23.51824675542132 26.342042886102686 26.274011212610677 23.86569914586532 76-77 23.63837433069721 26.349023386122262 26.211258299560974 23.801343983619557 78-79 23.663259228646787 26.272743805962946 26.311868988889227 23.75212797650104 80-81 23.426460918591474 26.602654591432216 26.323998863359844 23.646885626616463 82-83 23.75600972702543 26.62749130033028 26.11857237577069 23.4979265968736 84-85 23.829992376545448 26.235027159130208 26.001277914824637 23.933702549499706 86-87 23.500930475136336 26.153805223596134 26.524986135759924 23.820278165507606 88-89 23.82125070190956 26.243480957606497 26.322961893192304 23.61230644729164 90-91 23.70534646995688 26.43981808428676 26.28838991011132 23.566445535645045 92-93 23.63672818894719 26.21031301323211 26.32914714487234 23.82381165294836 94-95 23.686255928301016 26.331071143281694 26.318755276385712 23.66391765203158 96-97 23.557749849016858 26.35376967194584 26.341525936313314 23.746954542723987 98-99 23.576683486302684 26.14440432054257 26.23361128643506 24.045300906719685 100-101 23.58563201234094 26.370895887530153 25.989827161716672 24.053644938412234 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 78.0 1 63.5 2 92.5 3 159.0 4 147.5 5 133.5 6 246.5 7 489.0 8 778.5 9 933.0 10 938.0 11 994.0 12 1173.0 13 1409.5 14 1648.0 15 1835.0 16 1984.0 17 2090.5 18 2186.0 19 2312.5 20 2376.5 21 2435.0 22 2666.0 23 3109.5 24 3798.5 25 4631.5 26 6135.5 27 8150.5 28 10069.0 29 12561.0 30 15681.0 31 19283.0 32 23036.0 33 27112.0 34 31758.0 35 36340.0 36 41632.0 37 46740.0 38 49779.0 39 52963.0 40 56630.5 41 61683.5 42 67110.5 43 71722.0 44 76018.5 45 79280.0 46 81704.5 47 83063.0 48 86383.5 49 87730.0 50 85855.0 51 84064.0 52 84011.0 53 85143.0 54 85909.5 55 82836.0 56 75232.0 57 68666.0 58 63624.0 59 57186.0 60 49512.5 61 41502.0 62 34314.5 63 28164.0 64 22376.0 65 17528.5 66 13160.5 67 10841.0 68 8115.5 69 5221.5 70 3996.0 71 2964.5 72 2366.0 73 1982.5 74 1519.0 75 1242.5 76 839.5 77 436.5 78 240.0 79 140.5 80 104.0 81 79.0 82 53.0 83 28.0 84 21.0 85 25.5 86 23.0 87 18.0 88 20.5 89 19.0 90 14.5 91 20.0 92 22.0 93 18.5 94 21.0 95 26.0 96 25.0 97 14.5 98 8.5 99 10.0 100 7.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04779063048028899 2 0.012735039067813397 3 0.011046162919035277 4 0.00588824387006426 5 0.008809543154437225 6 0.006162115677974225 7 0.005933889171382587 8 0.010315838097942037 9 0.01593021016009633 10-11 0.002761540729758819 12-13 0.009653981228826287 14-15 0.009151882914324682 16-17 0.019376430409630063 18-19 0.01239269930792594 20-21 0.00360597880414788 22-23 0.01040712870057869 24-25 0.02284547330982296 26-27 0.008124863634662312 28-29 0.01880586414315097 30-31 0.015679161002845526 32-33 0.01401310750472657 34-35 0.2657241216246441 36-37 0.4690739389977935 38-39 0.46633522091869384 40-41 0.4869212518132596 42-43 0.47633154190740756 44-45 0.49207917086223063 46-47 0.49787612412965826 48-49 0.5047685646287257 50-51 0.5162711805609442 52-53 0.504243643663565 54-55 0.4912803780891599 56-57 0.48591705518425643 58-59 0.4842053563848191 60-61 0.4810101852925362 62-63 0.47861380697332395 64-65 0.4948178889413303 66-67 0.4690739389977935 68-69 0.495342809906491 70-71 0.47733573853641076 72-73 0.4853921342190956 74-75 0.49931395112118554 76-77 0.48591705518425643 78-79 0.48495850385657147 80-81 0.4882449655514911 82-83 0.4881536749488545 84-85 0.4880395616955586 86-87 0.49009360025488335 88-89 0.5038328359516999 90-91 0.5274314567332753 92-93 0.515677791643806 94-95 0.48801673904489945 96-97 0.46097189801379035 98-99 0.45291550233110556 100-101 0.4608806074111537 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2190806.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.7470831096523 #Duplication Level Percentage of deduplicated Percentage of total 1 74.6980617592088 38.65406809983718 2 13.41869642046295 13.887567977857804 3 4.853663666426691 7.53488811398645 4 2.2770825492628175 4.713295196969678 5 1.2402098739818115 3.2088621711174103 6 0.7370327716216621 2.2883577652586125 7 0.5166190716378961 1.871347102525434 8 0.3518259024353867 1.4564771370761902 9 0.2630884381384722 1.2252653346186126 >10 1.4646622936693159 14.075207807475998 >50 0.11716568334028746 4.150395696825424 >100 0.058975768470840025 5.277284587038248 >500 0.001590437096243432 0.5740915746741005 >1k 0.001325364246869502 1.0828914347388667 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3201 0.14611060951996663 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2214 0.1010586971187773 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.564530131832759E-5 2 0.0 0.0 0.0 0.0 4.564530131832759E-5 3 0.0 0.0 0.0 0.0 4.564530131832759E-5 4 0.0 0.0 0.0 0.0 4.564530131832759E-5 5 0.0 0.0 0.0 0.0 4.564530131832759E-5 6 0.0 0.0 0.0 0.0 4.564530131832759E-5 7 0.0 0.0 0.0 0.0 4.564530131832759E-5 8 0.0 0.0 0.0 0.0 4.564530131832759E-5 9 0.0 0.0 0.0 1.8258120527331036E-4 4.564530131832759E-5 10-11 0.0 0.0 0.0 1.8258120527331036E-4 4.564530131832759E-5 12-13 0.0 0.0 0.0 2.0540385593247415E-4 4.564530131832759E-5 14-15 0.0 0.0 0.0 2.2822650659163796E-4 9.129060263665518E-5 16-17 0.0 0.0 0.0 2.7387180790996557E-4 9.129060263665518E-5 18-19 0.0 0.0 0.0 2.9669445856912935E-4 9.129060263665518E-5 20-21 0.0 0.0 0.0 3.4233975988745693E-4 9.129060263665518E-5 22-23 0.0 0.0 0.0 3.8798506120578456E-4 9.129060263665518E-5 24-25 0.0 0.0 0.0 5.477436158199311E-4 9.129060263665518E-5 26-27 0.0 0.0 0.0 5.933889171382587E-4 9.129060263665518E-5 28-29 0.0 0.0 0.0 8.90083375707388E-4 1.3693590395498278E-4 30-31 0.0 0.0 0.0 0.0021453291619613966 1.3693590395498278E-4 32-33 0.0 0.0 0.0 0.004678643385128578 1.3693590395498278E-4 34-35 0.0 0.0 0.0 0.007622765320160707 1.3693590395498278E-4 36-37 0.0 0.0 0.0 0.013579477142202458 1.3693590395498278E-4 38-39 0.0 0.0 0.0 0.023347571624324562 1.5975855461414657E-4 40-41 0.0 0.0 0.0 0.035854384185546324 1.8258120527331036E-4 42-43 0.0 0.0 0.0 0.04763087192567484 1.8258120527331036E-4 44-45 0.0 0.0 0.0 0.06189502858765221 1.8258120527331036E-4 46-47 0.0 0.0 0.0 0.08042702092289322 1.8258120527331036E-4 48-49 0.0 0.0 0.0 0.09921006241538502 1.8258120527331036E-4 50-51 0.0 0.0 0.0 0.12107416174686395 1.8258120527331036E-4 52-53 0.0 0.0 0.0 0.14371423120075444 2.2822650659163796E-4 54-55 0.0 0.0 0.0 0.16849962981660632 3.1951710922829314E-4 56-57 0.0 0.0 0.0 0.19896786844658998 3.651624105466207E-4 58-59 0.0 0.0 0.0 0.23427450901631636 3.651624105466207E-4 60-61 0.0 0.0 0.0 0.27400874381392054 3.651624105466207E-4 62-63 0.0 0.0 0.0 0.31406249572075295 3.651624105466207E-4 64-65 0.0 0.0 0.0 0.3586579551087591 3.651624105466207E-4 66-67 0.0 0.0 0.0 0.4081831070391445 4.1080771186494835E-4 68-69 0.0 0.0 0.0 0.46747635345165206 4.1080771186494835E-4 70-71 0.0 0.0 0.0 0.5335707497605904 4.1080771186494835E-4 72-73 0.0 0.0 0.0 0.6028146718604934 4.564530131832759E-4 74-75 0.0 0.0 0.0 0.6706435896195282 4.564530131832759E-4 76-77 0.0 0.0 0.0 0.7499979459614408 4.564530131832759E-4 78-79 0.0 0.0 0.0 0.8432284739041248 4.564530131832759E-4 80-81 0.0 0.0 0.0 0.9472084703072751 4.564530131832759E-4 82-83 0.0 0.0 0.0 1.0573734050390586 4.564530131832759E-4 84-85 0.0 0.0 0.0 1.1664428525392023 4.564530131832759E-4 86-87 0.0 0.0 0.0 1.283842567529941 4.564530131832759E-4 88-89 0.0 0.0 0.0 1.4189298367815315 4.564530131832759E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2395 0.0 46.457947 1 GTATCAA 4270 0.0 40.583626 1 ATCAACG 5175 0.0 32.835445 3 TCAACGC 5280 0.0 32.091354 4 CAACGCA 5370 0.0 31.465376 5 TATCAAC 5640 0.0 30.296114 2 AACGCAG 5615 0.0 30.089693 6 GTACATG 7560 0.0 29.87406 1 ACATGGG 7330 0.0 29.122683 3 TACATGG 7635 0.0 28.331266 2 ACGCAGA 6700 0.0 25.006197 7 CGCAGAG 6775 0.0 24.730501 8 CATGGGG 4825 0.0 22.855862 4 GCAGAGT 7725 0.0 21.26033 9 GTACCTG 1985 0.0 21.228884 1 TACCTGG 2000 0.0 20.826271 2 GTATAGG 720 0.0 20.385757 1 GAGTACA 5920 0.0 20.154705 1 AGTACAT 5175 0.0 19.847609 2 GTCCAAG 1540 0.0 19.67691 1 >>END_MODULE