FastQCFastQC Report
Thu 26 May 2016
SRR936300_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936300_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2190806
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34650.15816096906800511No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24760.11301776606417913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26950.042.0101051
GTATCAA43350.037.8586921
ATCAACG52450.030.838543
TCAACGC54000.029.8655194
CAACGCA54500.029.6785585
TATCAAC55900.028.9431932
AACGCAG56550.028.6865546
GTACATG75100.027.617361
TACATGG73500.027.1122652
ACATGGG72700.026.2939343
ACGCAGA68450.023.4915247
CATGGGG46700.023.2596684
GTACCTG20350.022.9085331
CGCAGAG70300.022.8058518
TGGTATC16300.022.413362
TACCTGG19750.022.1016452
GTGGTAT17550.021.9555071
GTACTAG4500.021.1423421
GTGTAGC13800.021.0274371
GAGTACA57550.020.7474161
Produced by FastQC (version 0.11.5)