##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936300_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2190806 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52920888476661 34.0 31.0 34.0 31.0 34.0 2 32.661404980632696 34.0 31.0 34.0 31.0 34.0 3 32.747433592933376 34.0 31.0 34.0 31.0 34.0 4 36.21515095357599 37.0 35.0 37.0 35.0 37.0 5 36.17729867455174 37.0 35.0 37.0 35.0 37.0 6 36.21301018894416 37.0 37.0 37.0 35.0 37.0 7 36.16453442249108 37.0 37.0 37.0 35.0 37.0 8 36.14300307740621 37.0 37.0 37.0 35.0 37.0 9 37.92103956260846 39.0 38.0 39.0 35.0 39.0 10-11 37.85580170037876 39.0 38.0 39.0 35.0 39.0 12-13 37.80001766473161 39.0 38.0 39.0 35.0 39.0 14-15 39.181920717763234 41.0 39.0 41.0 36.0 41.0 16-17 39.10751020400711 40.0 38.0 41.0 36.0 41.0 18-19 39.112436929604904 40.0 38.5 41.0 36.0 41.0 20-21 39.06349854802296 40.0 39.0 41.0 35.5 41.0 22-23 38.946392332319704 40.0 38.0 41.0 35.0 41.0 24-25 39.104103923396224 40.5 39.0 41.0 35.5 41.0 26-27 39.03408448762693 40.5 39.0 41.0 35.0 41.0 28-29 38.94558714920445 40.0 38.5 41.0 35.0 41.0 30-31 38.84749060391472 40.0 38.0 41.0 35.0 41.0 32-33 38.716588324114504 40.0 38.0 41.0 35.0 41.0 34-35 38.59228156212828 40.0 38.0 41.0 34.0 41.0 36-37 38.45836304081694 40.0 38.0 41.0 34.0 41.0 38-39 38.33692029326193 40.0 38.0 41.0 34.0 41.0 40-41 38.20305357936759 40.0 38.0 41.0 33.5 41.0 42-43 38.24705450870593 40.0 38.0 41.0 34.0 41.0 44-45 38.14350837089181 40.0 38.0 41.0 33.0 41.0 46-47 37.96962989876785 40.0 38.0 41.0 33.0 41.0 48-49 37.77029253160709 40.0 37.0 41.0 33.0 41.0 50-51 37.58125799363339 40.0 37.0 41.0 33.0 41.0 52-53 37.36902172077309 40.0 36.5 41.0 32.0 41.0 54-55 37.17317599093667 40.0 36.0 41.0 32.0 41.0 56-57 36.9228792964781 39.0 35.5 41.0 31.0 41.0 58-59 36.662753799286655 39.0 35.0 41.0 31.0 41.0 60-61 36.38048804869076 39.0 35.0 41.0 31.0 41.0 62-63 36.082179115814 38.0 35.0 40.0 30.5 41.0 64-65 35.71060605092372 37.5 35.0 40.0 30.0 41.0 66-67 35.36033907155631 37.0 35.0 40.0 29.0 41.0 68-69 35.00213186379807 36.5 34.0 39.0 29.0 41.0 70-71 34.62816721334522 36.0 34.0 39.0 29.0 40.5 72-73 34.212911823319814 35.5 34.0 38.5 28.5 40.0 74-75 33.78347352526879 35.0 34.0 37.0 28.0 39.5 76-77 33.05857410469024 35.0 33.0 37.0 27.0 39.0 78-79 33.10522793894119 35.0 33.0 36.0 27.0 39.0 80-81 32.9337787097534 35.0 33.0 36.0 27.0 37.5 82-83 32.65138469586079 35.0 33.0 36.0 27.0 37.0 84-85 32.36443322685806 35.0 33.0 35.0 26.5 37.0 86-87 31.961097879045433 35.0 33.0 35.0 26.0 36.0 88-89 31.479279315466545 35.0 32.0 35.0 24.5 36.0 90-91 31.271506240169145 35.0 32.0 35.0 24.0 36.0 92-93 30.988085435223383 34.0 32.0 35.0 23.0 35.0 94-95 30.83511319578274 34.0 32.0 35.0 23.0 35.0 96-97 30.58677263071217 34.0 32.0 35.0 20.0 35.0 98-99 30.14591479117731 34.0 31.0 35.0 10.0 35.0 100-101 26.76422239121127 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05692394484145069 1101 2 0.079069740591315 1101 3 0.17120533152398565 1101 4 0.15756904971988206 1101 5 0.08832499469294675 1101 6 0.13769407113869647 1101 7 0.12879259565498558 1101 8 0.07775030282073914 1101 9 0.21217969581779528 1101 10-11 0.1087005049939549 1101 12-13 0.20216588737844887 1101 14-15 0.25698085122582626 1101 16-17 0.3588877147944771 1101 18-19 0.29825841406125875 1101 20-21 0.34134003824128456 1101 22-23 0.30146687460351984 1101 24-25 0.2935389297234181 1101 26-27 0.29343369419071763 1101 28-29 0.24496240390471513 1101 30-31 0.23960647966335813 1101 32-33 0.2932647374144537 1101 34-35 0.3088470094804947 1101 36-37 0.27227689937223687 1101 38-39 0.242736839452931 1101 40-41 0.21980134051523237 1101 42-43 0.1690681852663829 1101 44-45 0.28087441074149666 1101 46-47 0.2146019302871025 1101 48-49 0.19874867505767924 1101 50-51 0.15373213121994667 1101 52-53 0.20933108348377516 1101 54-55 0.24843698126348812 1101 56-57 0.28401942485435683 1101 58-59 0.21837567576892525 1101 60-61 0.35348693683241805 1101 62-63 0.32113344689347656 1101 64-65 0.4140872764940795 1101 66-67 0.35106757923416865 1101 68-69 0.39965943851017016 1101 70-71 0.4032429796356709 1101 72-73 0.19724833244625017 1101 74-75 0.11323721566547107 1101 76-77 0.01351666969348031 1101 78-79 -0.06218040809488912 1101 80-81 0.15348946531960195 1101 82-83 0.10278756167559777 1101 84-85 0.16597573298381718 1101 86-87 0.19593900948995824 1101 88-89 0.1763626036083572 1101 90-91 0.4672165162178814 1101 92-93 0.5586254926860903 1101 94-95 0.3219415005804116 1101 96-97 0.2316593027773166 1101 98-99 0.3130089489398671 1101 100-101 0.6502452639381744 1102 1 0.10753744390295594 1102 2 0.1877200259987859 1102 3 0.11821984523189855 1102 4 0.1004589596959704 1102 5 0.1229533986292708 1102 6 0.15287739904455577 1102 7 0.10954823904958033 1102 8 0.08652365727541422 1102 9 0.03179151055737606 1102 10-11 0.048895555413956515 1102 12-13 0.1827670351159938 1102 14-15 0.19580903845179876 1102 16-17 0.20921445992217258 1102 18-19 0.2336782577444083 1102 20-21 0.19877635979546682 1102 22-23 0.18812119827484253 1102 24-25 0.18115701201726608 1102 26-27 0.21183833289158827 1102 28-29 0.1342313548861469 1102 30-31 0.1562008956656129 1102 32-33 0.1435481737865274 1102 34-35 0.20680311383572558 1102 36-37 0.07161841195274121 1102 38-39 0.13586998639203784 1102 40-41 0.060936496314312194 1102 42-43 0.11193824539614639 1102 44-45 0.012942850790658156 1102 46-47 0.07972208571001005 1102 48-49 0.13482273080881413 1102 50-51 0.10276799781009771 1102 52-53 0.18329627490430767 1102 54-55 0.18924918145099667 1102 56-57 0.3296451988942479 1102 58-59 0.09006238245176945 1102 60-61 0.07046310841060688 1102 62-63 0.20026745501053966 1102 64-65 0.2040489732311812 1102 66-67 0.12092832215144966 1102 68-69 0.011174903483791354 1102 70-71 0.1003745796748774 1102 72-73 0.04099228553065615 1102 74-75 0.14336789428313068 1102 76-77 0.056545167687524156 1102 78-79 0.061511896522233656 1102 80-81 0.16174103255520578 1102 82-83 0.12621343415962372 1102 84-85 0.21536478802841685 1102 86-87 0.07936252775445851 1102 88-89 0.11256464228474172 1102 90-91 0.2860378073573848 1102 92-93 0.2685052747986276 1102 94-95 0.2795756820713038 1102 96-97 0.1433352600049247 1102 98-99 0.22387564320198194 1102 100-101 0.4951532786398296 1103 1 0.045034992800111695 1103 2 0.10759166073530935 1103 3 0.11560461922275778 1103 4 0.03346689401661962 1103 5 0.1142763602384207 1103 6 0.1289226701754771 1103 7 0.14799200766117337 1103 8 0.1311890636051487 1103 9 0.16911961773651285 1103 10-11 0.1522570180065017 1103 12-13 0.11455519321360441 1103 14-15 0.2768290679241261 1103 16-17 0.22215010484023878 1103 18-19 0.1507177606580612 1103 20-21 0.19706313963458655 1103 22-23 0.14902133994186784 1103 24-25 0.15145837544826435 1103 26-27 0.17281261202560216 1103 28-29 0.15628416225175812 1103 30-31 0.2430690550740664 1103 32-33 0.25799291907866717 1103 34-35 0.29045247155276144 1103 36-37 0.40083093774639167 1103 38-39 0.33951150595224533 1103 40-41 0.3145047648337709 1103 42-43 0.34017312363610586 1103 44-45 0.3634078485047496 1103 46-47 0.3839122762486724 1103 48-49 0.39781908249332076 1103 50-51 0.3855876605705788 1103 52-53 0.3196250036562702 1103 54-55 0.36406425413525767 1103 56-57 0.383757491369785 1103 58-59 0.40504534963734784 1103 60-61 0.3027650713880732 1103 62-63 0.3188080267393474 1103 64-65 0.3921953592714118 1103 66-67 0.2907980765898941 1103 68-69 0.3277088479677275 1103 70-71 0.22646213065110032 1103 72-73 0.26911944955227085 1103 74-75 0.3354702931674183 1103 76-77 0.340419140440396 1103 78-79 0.37202257473501277 1103 80-81 0.4303886201059086 1103 82-83 0.48646914395334306 1103 84-85 0.4769040430113378 1103 86-87 0.25846870926124055 1103 88-89 0.4224471666332583 1103 90-91 0.6688448721207578 1103 92-93 0.7259433847874703 1103 94-95 0.6711736775661095 1103 96-97 0.6255426708505922 1103 98-99 0.5631211709130106 1103 100-101 0.83706278580296 1104 1 0.031795663787569595 1104 2 0.13287242185541004 1104 3 0.12980593094696502 1104 4 0.014610535819379322 1104 5 0.13774311156575436 1104 6 0.12996879733684352 1104 7 0.12112334269288283 1104 8 0.09127026558022067 1104 9 0.045403788513610266 1104 10-11 0.14558289190855334 1104 12-13 0.22918393809330695 1104 14-15 0.2608734992909092 1104 16-17 0.23445635966370304 1104 18-19 0.2956226335509058 1104 20-21 0.2389766257184931 1104 22-23 0.2824732756513768 1104 24-25 0.2283022616828987 1104 26-27 0.3044807370239866 1104 28-29 0.26012551124731687 1104 30-31 0.18702678668424255 1104 32-33 0.20023252943307313 1104 34-35 0.22152732655845142 1104 36-37 0.19707799204537935 1104 38-39 0.09343996768329532 1104 40-41 0.2217787427755411 1104 42-43 0.34753937654954115 1104 44-45 0.30202627923657843 1104 46-47 0.36089528474829535 1104 48-49 0.23199837728024164 1104 50-51 0.34588361157063474 1104 52-53 0.25697107222624993 1104 54-55 0.24326999247698922 1104 56-57 0.33684747688970873 1104 58-59 0.433778872424746 1104 60-61 0.3511181889915562 1104 62-63 0.21862438018732888 1104 64-65 0.1558015942202715 1104 66-67 0.16608503239341132 1104 68-69 0.30088509445418055 1104 70-71 0.28773774502173666 1104 72-73 0.16266226642968462 1104 74-75 0.1457819889878138 1104 76-77 0.15850193754644692 1104 78-79 0.2436856407419441 1104 80-81 0.319759317704694 1104 82-83 0.3038594270761905 1104 84-85 0.4403556689866761 1104 86-87 0.3699748174771962 1104 88-89 0.45992835673518684 1104 90-91 0.5448966132139645 1104 92-93 0.6327237238947632 1104 94-95 0.6439706287494218 1104 96-97 0.6458685886316573 1104 98-99 0.6382727470727723 1104 100-101 0.8272652487058423 1105 1 0.014011232426845766 1105 2 0.033601212628774135 1105 3 0.08945244693977372 1105 4 0.08656978014117556 1105 5 0.0778660787161769 1105 6 0.04844850328819916 1105 7 0.06449812437219293 1105 8 0.10012449625348552 1105 9 0.11293070387035442 1105 10-11 0.09770688688858087 1105 12-13 0.11497754544005545 1105 14-15 0.08984116362260863 1105 16-17 0.06969676379438994 1105 18-19 0.06668178216538223 1105 20-21 0.07403418045227994 1105 22-23 -0.08059710460833003 1105 24-25 0.07377068757676142 1105 26-27 0.12704643805218296 1105 28-29 0.016892242339537233 1105 30-31 0.07880005343209007 1105 32-33 0.093883447217884 1105 34-35 0.08998487458899973 1105 36-37 0.09573399311789643 1105 38-39 0.20092450368898795 1105 40-41 0.18011067960125615 1105 42-43 0.2566371581506388 1105 44-45 0.22048140665392424 1105 46-47 0.1853527880906043 1105 48-49 0.15887165259334068 1105 50-51 0.06313550802797607 1105 52-53 0.1661202453075461 1105 54-55 0.13732384925862107 1105 56-57 0.2525036306470625 1105 58-59 0.16198087818208506 1105 60-61 0.228725654907457 1105 62-63 0.2201583041502886 1105 64-65 0.2273731680284925 1105 66-67 0.15559856362117586 1105 68-69 0.18068079470033638 1105 70-71 0.18032359299977685 1105 72-73 0.1447731900984479 1105 74-75 0.1754810509071021 1105 76-77 0.03491846176934388 1105 78-79 -0.08947973927570985 1105 80-81 -0.003146758779237757 1105 82-83 0.007013672273131988 1105 84-85 0.01867814680082347 1105 86-87 0.17528266361557954 1105 88-89 0.2400588464066189 1105 90-91 0.32513547187622294 1105 92-93 0.2327285651211568 1105 94-95 0.14664447532723557 1105 96-97 0.047378306462640296 1105 98-99 0.028949662243313412 1105 100-101 0.23405972374932205 1106 1 -0.002242801516317172 1106 2 -0.02333107516840016 1106 3 -0.053016848658295146 1106 4 -0.06235188977586148 1106 5 -0.001137537322087212 1106 6 0.026956872994738035 1106 7 0.008242997298147259 1106 8 0.02460898346868845 1106 9 -0.0015962800170896685 1106 10-11 0.0904260789777922 1106 12-13 0.07835826551342251 1106 14-15 0.08192539661233411 1106 16-17 -0.055143861592561905 1106 18-19 0.11198262532722225 1106 20-21 0.12076177707344016 1106 22-23 0.01888131234467494 1106 24-25 0.04848332958703594 1106 26-27 -0.010851538978521091 1106 28-29 0.018748509171814476 1106 30-31 0.07993006538881531 1106 32-33 0.06806911307969443 1106 34-35 0.08854264317029248 1106 36-37 9.411656042743743E-4 1106 38-39 0.048336975828661366 1106 40-41 0.0010395167808638917 1106 42-43 0.07132124332585477 1106 44-45 0.005052154471535175 1106 46-47 0.012897727378309298 1106 48-49 -0.01996637346380936 1106 50-51 -0.0840994270806803 1106 52-53 -0.03119979439342302 1106 54-55 0.07135473068971265 1106 56-57 0.06897675026846883 1106 58-59 -0.04556658594774632 1106 60-61 -0.013431735624671148 1106 62-63 -0.09024116747087874 1106 64-65 -0.05297608728818659 1106 66-67 -0.055688762445250006 1106 68-69 0.06304733599553458 1106 70-71 -0.06846477299205134 1106 72-73 -0.21123414451955824 1106 74-75 0.01669278648937933 1106 76-77 -0.17024962886959116 1106 78-79 -0.2683897136596869 1106 80-81 -0.16502686650583342 1106 82-83 -0.134435529064163 1106 84-85 -0.021791275523035836 1106 86-87 -0.057493511548489806 1106 88-89 -0.02015539757245577 1106 90-91 0.16076208255137203 1106 92-93 0.16761992435685613 1106 94-95 0.08643726898282011 1106 96-97 0.19150966887131915 1106 98-99 -0.21502590483042283 1106 100-101 0.08897090444675726 1107 1 -0.07840231177148382 1107 2 0.024640601995685074 1107 3 0.052123352407598134 1107 4 -0.045190128632448534 1107 5 -3.812691534790247E-4 1107 6 0.05631178659292857 1107 7 0.03446483596093941 1107 8 0.017010973940266183 1107 9 0.07494275220189905 1107 10-11 -0.014027374066458265 1107 12-13 0.03471715097045802 1107 14-15 0.09280410098894976 1107 16-17 0.10144553803599621 1107 18-19 0.13597351074388797 1107 20-21 0.03990530400334791 1107 22-23 0.04143851821515199 1107 24-25 0.09948992075305085 1107 26-27 0.12171568485869244 1107 28-29 0.10352082122768991 1107 30-31 -0.027818465982313967 1107 32-33 0.041212887304503454 1107 34-35 0.08132604816868394 1107 36-37 -0.059235271734223716 1107 38-39 0.09768896226277235 1107 40-41 0.10540641661128802 1107 42-43 -0.02993920362182223 1107 44-45 0.030758963660808547 1107 46-47 0.02823417460860611 1107 48-49 -0.06833384863337955 1107 50-51 -0.020307058904002417 1107 52-53 0.013466963116265163 1107 54-55 0.0909512110272459 1107 56-57 -0.032544996541936655 1107 58-59 -0.07234496429884274 1107 60-61 0.009529721699294669 1107 62-63 -0.0346017082896779 1107 64-65 -0.030248803090842102 1107 66-67 -0.1743901866619808 1107 68-69 -0.035558728280953744 1107 70-71 0.023486030464020757 1107 72-73 -0.02439452283028487 1107 74-75 -0.17923403945538752 1107 76-77 -0.22261558494194134 1107 78-79 -0.1550728834755759 1107 80-81 -0.07023116237146354 1107 82-83 0.06100616990224239 1107 84-85 -0.07053978670682426 1107 86-87 0.10638755474672834 1107 88-89 0.1550401672692736 1107 90-91 -0.18908562921380678 1107 92-93 -0.02323205703041964 1107 94-95 -0.11132458603171358 1107 96-97 -0.17564061072332038 1107 98-99 -0.026167423120153188 1107 100-101 0.4311179035988886 1108 1 -0.0919925804078261 1108 2 -0.032311765026889816 1108 3 -0.0682327979507491 1108 4 -0.021126211709827203 1108 5 -0.050041207723033665 1108 6 -0.10198254327884371 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2316 64-65 -0.5151637389392789 2316 66-67 -0.6327332484115331 2316 68-69 -0.5491356779535437 2316 70-71 -0.4555019902862014 2316 72-73 -0.5362116789972546 2316 74-75 -0.4927020868572711 2316 76-77 -0.4088454667171888 2316 78-79 -0.4760467889237674 2316 80-81 -0.5251495416593244 2316 82-83 -0.6824419167492479 2316 84-85 -0.5106494168590316 2316 86-87 -0.7305367539758088 2316 88-89 -0.6490219714145447 2316 90-91 -0.7053750572949156 2316 92-93 -0.6261819367899939 2316 94-95 -0.46650192316123196 2316 96-97 -0.38477356387854655 2316 98-99 -0.5215599332195069 2316 100-101 -0.35172284992643554 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 12.0 5 37.0 6 51.0 7 134.0 8 295.0 9 613.0 10 987.0 11 1583.0 12 2309.0 13 3423.0 14 4366.0 15 5453.0 16 6976.0 17 9333.0 18 9072.0 19 6687.0 20 6355.0 21 6820.0 22 7241.0 23 8358.0 24 10067.0 25 12143.0 26 15244.0 27 18815.0 28 23696.0 29 30136.0 30 38032.0 31 46255.0 32 58881.0 33 76572.0 34 106914.0 35 159956.0 36 276259.0 37 520881.0 38 602536.0 39 114130.0 40 176.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.57947899031202 17.847810833856247 13.05897413716953 24.513736038662202 2 16.257589585529534 18.701140509428456 39.60280997855581 25.438459926486196 3 20.165181216410762 22.54635965028396 28.040684569971052 29.24777456333422 4 13.001835854019022 14.969239631441578 35.47374801785279 36.555176496686606 5 14.510869515602934 36.590688541112264 33.756024038641485 15.142417904643313 6 35.380266440752855 34.14341571093013 16.397298528486775 14.079019319830236 7 31.277255950549705 30.647807245369968 19.220916868038522 18.854019936041805 8 27.527174930139864 35.45562683322942 18.338182385843382 18.679015850787337 9 26.7106261348563 16.00844620655594 19.081926925524215 38.199000733063535 10-11 26.460906026023395 25.486991498075128 26.45622736555356 21.59587511034792 12-13 26.478995710475957 22.244086356483525 27.40671511923142 23.870202813809094 14-15 23.013699067831656 24.232314499777708 24.889561193460306 27.86442523893033 16-17 23.73859717591103 27.566819350966203 25.37021948653343 23.324363986589344 18-19 23.434662859239932 26.290027505858575 27.095347557017828 23.179962077883665 20-21 23.919238837039618 25.72499019196364 27.133262172292337 23.222508798704403 22-23 23.516076621133188 25.5408113591097 27.102223360311996 23.840888659445124 24-25 23.436810313858974 25.74671541076366 27.31530276465575 23.501171510721615 26-27 23.503271237711463 26.09221179307099 27.079654056284554 23.324862912932996 28-29 23.23013843385222 26.019379141689576 27.37690045566431 23.3735819687939 30-31 23.483094754413315 25.71065296472565 27.26333877202436 23.542913508836673 32-33 23.265844371269022 25.81660487735407 27.42459528076327 23.492955470613637 34-35 23.70455542404439 25.948649175428034 27.0149166527066 23.331878747820976 36-37 23.802232512706517 25.92220667745418 26.89315114763751 23.382409662201795 38-39 23.51787825790857 26.01397158622396 27.07100385771704 23.397146298150428 40-41 23.605837150440824 25.809785945284702 27.17273866336193 23.411638240912545 42-43 23.64021874087058 25.773010699678643 26.96999160093485 23.616778958515923 44-45 23.495379272583776 25.98971163219142 26.916344874634053 23.59856422059075 46-47 23.41490371128519 26.28328459684144 26.66942552264381 23.632386169229562 48-49 23.893098720981033 26.046801037385407 26.68176819339084 23.37833204824271 50-51 23.621408175017283 26.122108298015256 26.60405767781013 23.65242584915733 52-53 23.706502442861062 26.256796439809026 26.43149193237317 23.605209184956742 54-55 23.661446650946367 25.991830374137642 26.572029917865546 23.774693057050445 56-57 23.53394285103927 26.354371576655193 26.573041131832337 23.5386444404732 58-59 23.800250093631057 26.138778457622045 26.567507136158614 23.493464312588284 60-61 23.774985113746045 26.109702048973894 26.770996350842246 23.34431648643782 62-63 23.52227901512046 26.286216123198493 26.613150593891017 23.57835426779003 64-65 23.562154504786296 26.130067900272348 26.615578469193597 23.692199125747763 66-67 23.55297548025704 26.35155737203568 26.429451078735404 23.666016068971878 68-69 23.268217267982653 26.120295452906372 26.770375834281996 23.84111144482898 70-71 23.426958060583267 26.05944030567787 26.71408611363048 23.79951552010838 72-73 23.53592035313603 26.192702521784 26.50645058439485 23.764926540685117 74-75 23.45931132195183 26.328529317520584 26.32239002449327 23.889769336034316 76-77 23.526549335309056 26.34032953852944 26.31023636631806 23.82288475984345 78-79 23.466082637052722 26.282909068809836 26.452342237637332 23.79866605650011 80-81 23.33450491264507 26.546313519188413 26.444796932592347 23.67438463557417 82-83 23.608525811961442 26.46208746917801 26.303036416734297 23.62635030212625 84-85 23.666031584895705 26.1725429227942 26.136551405563463 24.02487408674663 86-87 23.400503484793887 26.115126046003713 26.569642522140285 23.91472794706212 88-89 23.615701469059935 26.237892990692718 26.36094021856034 23.785465321687006 90-91 23.540585096068767 26.376323267787207 26.40395561517 23.679136020974028 92-93 23.487414885328096 26.195839967478573 26.410566533876946 23.90617861331639 94-95 23.491722739267313 26.30108898232167 26.413629784353475 23.793558494057546 96-97 23.423142759328996 26.196005934040855 26.46280954011183 23.918041766518318 98-99 23.33202556112671 26.05013765843044 26.369777270188706 24.248059510254144 100-101 23.215350644460038 26.166047597039878 26.360224348550464 24.25837740994962 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 50.0 1 40.5 2 68.5 3 130.0 4 112.5 5 101.5 6 215.5 7 415.0 8 650.5 9 787.0 10 746.5 11 713.0 12 799.0 13 898.5 14 993.5 15 1086.0 16 1159.5 17 1312.5 18 1444.5 19 1521.0 20 1728.0 21 2047.5 22 2465.5 23 3149.5 24 4007.0 25 4972.5 26 6517.5 27 8642.0 28 10603.0 29 13002.5 30 16210.0 31 19869.5 32 23649.5 33 27465.0 34 31647.0 35 36330.0 36 41676.0 37 46882.0 38 50155.0 39 53022.5 40 56490.5 41 61967.0 42 67277.0 43 71615.5 44 76340.0 45 79615.0 46 81954.5 47 83783.5 48 86863.0 49 87816.5 50 86401.5 51 84898.0 52 84370.0 53 85337.0 54 86242.0 55 83255.5 56 75509.5 57 68739.0 58 64118.5 59 57224.5 60 49286.5 61 41602.5 62 34272.5 63 27819.5 64 22141.5 65 17279.0 66 12693.5 67 10467.0 68 7821.0 69 4980.0 70 3803.5 71 2868.0 72 2252.0 73 1926.5 74 1587.0 75 1260.0 76 784.0 77 380.0 78 214.5 79 122.5 80 73.0 81 37.0 82 21.0 83 12.5 84 5.5 85 3.5 86 3.5 87 3.0 88 2.5 89 1.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2708135727216376 2 0.02140764631829564 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.651624105466207E-4 12-13 1.5975855461414657E-4 14-15 0.0 16-17 5.020983145016036E-4 18-19 0.0 20-21 2.2822650659163795E-5 22-23 2.2822650659163795E-5 24-25 0.0020540385593247417 26-27 0.0020540385593247417 28-29 0.0022137971139388883 30-31 9.813739783440434E-4 32-33 0.0012780684369131726 34-35 0.0015062949435048106 36-37 4.564530131832759E-5 38-39 1.1411325329581897E-4 40-41 5.020983145016036E-4 42-43 0.0039254959133761735 44-45 0.0036288014548070432 46-47 0.0129860882250642 48-49 0.01364794509417995 50-51 0.005454613507540148 52-53 0.0062534062806108804 54-55 3.651624105466207E-4 56-57 0.0026246048258038368 58-59 0.0012096004849356814 60-61 0.0012096004849356814 62-63 0.0 64-65 8.900833757073881E-4 66-67 0.0 68-69 0.0 70-71 1.3693590395498278E-4 72-73 0.0016204081968006294 74-75 0.0 76-77 0.19460874217068969 78-79 0.1064676653249991 80-81 0.0014150043408681554 82-83 0.0 84-85 5.477436158199311E-4 86-87 0.12125674295213726 88-89 0.5833697734988857 90-91 0.3090871578770553 92-93 0.29212992843729657 94-95 0.0020540385593247417 96-97 0.002556136873826345 98-99 0.0026246048258038368 100-101 8.444380743890604E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2190806.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.758736422823446 #Duplication Level Percentage of deduplicated Percentage of total 1 72.8267240752519 36.2376576779815 2 14.411313934199057 14.341775431167472 3 5.1985833552621 7.7602481683969184 4 2.371415397291267 4.719945348113652 5 1.3793128746336358 3.4316432886751 6 0.8197032564488933 2.447243896954232 7 0.5423961295992826 1.8892262234643205 8 0.38415274450207876 1.5291964127826538 9 0.290254268837553 1.2998417092818424 >10 1.5790845650747867 14.594850739614692 >50 0.12927691636146413 4.415247077120648 >100 0.06438621922631538 5.501962333284174 >500 0.0019276089065834935 0.6469264247719572 >1k 0.0014686544050159472 1.184235268390772 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3465 0.15816096906800511 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2476 0.11301776606417913 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 9.129060263665518E-5 16-17 0.0 0.0 0.0 4.564530131832759E-5 9.129060263665518E-5 18-19 0.0 0.0 0.0 6.846795197749139E-5 1.1411325329581898E-4 20-21 0.0 0.0 0.0 1.1411325329581898E-4 1.3693590395498278E-4 22-23 0.0 0.0 0.0 1.5975855461414657E-4 1.5975855461414657E-4 24-25 0.0 0.0 0.0 3.1951710922829314E-4 1.8258120527331036E-4 26-27 0.0 0.0 0.0 3.651624105466207E-4 1.8258120527331036E-4 28-29 0.0 0.0 0.0 6.618568691157501E-4 1.8258120527331036E-4 30-31 0.0 0.0 0.0 0.0019399253060289226 1.8258120527331036E-4 32-33 0.0 0.0 0.0 0.004587352782491923 1.8258120527331036E-4 34-35 0.0 0.0 0.0 0.0075771200188423805 1.8258120527331036E-4 36-37 0.0 0.0 0.0 0.01346536388890664 1.8258120527331036E-4 38-39 0.0 0.0 0.0 0.023210635720369584 1.8258120527331036E-4 40-41 0.0 0.0 0.0 0.036014142740160474 1.8258120527331036E-4 42-43 0.0 0.0 0.0 0.04806450228819895 1.8258120527331036E-4 44-45 0.0 0.0 0.0 0.062465594854131314 1.8258120527331036E-4 46-47 0.0 0.0 0.0 0.08120299104530479 1.8258120527331036E-4 48-49 0.0 0.0 0.0 0.10019143639372907 1.8258120527331036E-4 50-51 0.0 0.0 0.0 0.12216964897850381 1.8258120527331036E-4 52-53 0.0 0.0 0.0 0.1448553637337126 1.8258120527331036E-4 54-55 0.0 0.0 0.0 0.16977769825351946 1.8258120527331036E-4 56-57 0.0 0.0 0.0 0.20038287278745814 1.8258120527331036E-4 58-59 0.0 0.0 0.0 0.2357123360078437 1.8258120527331036E-4 60-61 0.0 0.0 0.0 0.2757204426133578 1.8258120527331036E-4 62-63 0.0 0.0 0.0 0.3158883077734861 1.8258120527331036E-4 64-65 0.0 0.0 0.0 0.36073481631874293 1.8258120527331036E-4 66-67 0.0 0.0 0.0 0.41000891909187764 1.8258120527331036E-4 68-69 0.0 0.0 0.0 0.4695303920109768 1.8258120527331036E-4 70-71 0.0 0.0 0.0 0.5359214827784843 1.8258120527331036E-4 72-73 0.0 0.0 0.0 0.6053479860836606 1.8258120527331036E-4 74-75 0.0 0.0 0.0 0.673131258541377 1.8258120527331036E-4 76-77 0.0 0.0 0.0 0.7527823093418586 1.8258120527331036E-4 78-79 0.0 0.0 0.0 0.8460356599352019 1.8258120527331036E-4 80-81 0.0 0.0 0.0 0.9505405773035129 1.8258120527331036E-4 82-83 0.0 0.0 0.0 1.0606826893846375 1.8258120527331036E-4 84-85 0.0 0.0 0.0 1.169706491583463 1.8258120527331036E-4 86-87 0.0 0.0 0.0 1.287174674526179 1.8258120527331036E-4 88-89 0.0 0.0 0.0 1.4230150912495219 1.8258120527331036E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2695 0.0 42.010105 1 GTATCAA 4335 0.0 37.858692 1 ATCAACG 5245 0.0 30.83854 3 TCAACGC 5400 0.0 29.865519 4 CAACGCA 5450 0.0 29.678558 5 TATCAAC 5590 0.0 28.943193 2 AACGCAG 5655 0.0 28.686554 6 GTACATG 7510 0.0 27.61736 1 TACATGG 7350 0.0 27.112265 2 ACATGGG 7270 0.0 26.293934 3 ACGCAGA 6845 0.0 23.491524 7 CATGGGG 4670 0.0 23.259668 4 GTACCTG 2035 0.0 22.908533 1 CGCAGAG 7030 0.0 22.805851 8 TGGTATC 1630 0.0 22.41336 2 TACCTGG 1975 0.0 22.101645 2 GTGGTAT 1755 0.0 21.955507 1 GTACTAG 450 0.0 21.142342 1 GTGTAGC 1380 0.0 21.027437 1 GAGTACA 5755 0.0 20.747416 1 >>END_MODULE