FastQCFastQC Report
Thu 26 May 2016
SRR936299_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936299_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2200422
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35330.16056011074239396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25460.11570507838950891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52300.042.464281
GGTATCA29350.042.4326631
TCAACGC63850.034.4751744
ATCAACG65050.033.767193
CAACGCA66250.033.083355
TATCAAC66900.032.833422
AACGCAG68200.032.135966
TACATGG88400.027.2577362
TACCTGG19000.026.4104962
GTACATG93650.026.3946171
ACGCAGA82900.026.3810427
CGCAGAG84150.025.9329138
ACATGGG90950.025.6606983
GTACCTG21700.024.2223261
GTGTACG2653.274181E-1123.2300451
GCAGAGT98650.021.7373039
GTACGCT2005.569018E-721.3028053
CATGGGG52100.021.170574
GAGTACA70500.020.8221321
AGTACAT63450.020.741372
Produced by FastQC (version 0.11.5)