##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936299_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2200422 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78917316769238 33.0 31.0 34.0 30.0 34.0 2 31.87920180765326 34.0 31.0 34.0 30.0 34.0 3 31.953085362716788 34.0 31.0 34.0 30.0 34.0 4 35.45312899071178 37.0 35.0 37.0 33.0 37.0 5 35.43992379643541 37.0 35.0 37.0 33.0 37.0 6 35.528407278240266 37.0 35.0 37.0 33.0 37.0 7 35.47038658948147 37.0 35.0 37.0 33.0 37.0 8 35.47478029214396 37.0 35.0 37.0 33.0 37.0 9 37.15273797480665 39.0 37.0 39.0 34.0 39.0 10-11 37.07299077177014 39.0 37.0 39.0 33.5 39.0 12-13 36.97802944162529 39.0 37.0 39.0 33.0 39.0 14-15 38.23889054008731 40.0 38.0 41.0 33.0 41.0 16-17 38.17820331736367 40.0 38.0 41.0 33.0 41.0 18-19 38.11709708410478 40.0 38.0 41.0 33.0 41.0 20-21 38.03270349960144 40.0 38.0 41.0 33.0 41.0 22-23 37.869852464663595 40.0 38.0 41.0 33.0 41.0 24-25 38.029203034690624 40.0 38.0 41.0 33.0 41.0 26-27 37.9874199130894 40.0 38.0 41.0 33.0 41.0 28-29 37.861113913603845 40.0 38.0 41.0 33.0 41.0 30-31 37.71586972862478 40.0 38.0 41.0 33.0 41.0 32-33 37.53907659530763 40.0 37.5 41.0 32.0 41.0 34-35 37.27956523794072 40.0 37.0 41.0 31.5 41.0 36-37 37.090035002376815 40.0 37.0 41.0 31.0 41.0 38-39 36.92605213908968 40.0 37.0 41.0 31.0 41.0 40-41 36.74061679986839 40.0 36.0 41.0 30.0 41.0 42-43 36.551711671670255 40.0 36.0 41.0 30.0 41.0 44-45 36.37310365920719 40.0 36.0 41.0 30.0 41.0 46-47 36.10734395493228 39.0 35.5 41.0 29.5 41.0 48-49 35.93842317519094 39.0 35.0 41.0 28.5 41.0 50-51 35.63491412101861 38.5 35.0 40.5 28.5 41.0 52-53 35.64770280428027 39.0 35.0 40.5 28.0 41.0 54-55 35.71883620505521 39.0 35.0 41.0 28.0 41.0 56-57 35.546140240372075 39.0 35.0 41.0 28.0 41.0 58-59 35.2999004281906 38.5 35.0 41.0 28.0 41.0 60-61 34.997266887896956 38.0 35.0 40.0 27.0 41.0 62-63 34.683484122591025 37.5 34.0 40.0 26.5 41.0 64-65 34.3427947002893 37.0 34.0 40.0 26.0 41.0 66-67 33.983905814430145 36.5 34.0 39.5 26.0 41.0 68-69 33.600617972370756 36.0 34.0 39.0 26.0 41.0 70-71 33.203864076981596 35.0 33.0 39.0 25.0 40.5 72-73 32.80580315957575 35.0 33.0 37.5 24.0 40.0 74-75 32.39562002197761 35.0 33.0 37.0 23.0 39.0 76-77 32.005024490756774 35.0 32.5 36.5 22.0 39.0 78-79 31.620297833779155 35.0 32.0 36.0 20.5 38.5 80-81 31.24187065026618 35.0 32.0 36.0 19.5 37.0 82-83 30.907633399411566 35.0 31.5 35.0 18.0 37.0 84-85 30.604012548502062 35.0 31.0 35.0 17.5 36.5 86-87 30.28933427315306 34.0 31.0 35.0 11.5 36.0 88-89 29.969642414046035 34.0 31.0 35.0 7.0 36.0 90-91 29.624995114573476 34.0 30.0 35.0 2.0 35.5 92-93 29.320719616509926 34.0 30.0 35.0 2.0 35.0 94-95 28.94066660849601 34.0 29.5 35.0 2.0 35.0 96-97 28.507638307561002 34.0 29.0 35.0 2.0 35.0 98-99 27.997838596414688 34.0 29.0 35.0 2.0 35.0 100-101 24.64961334689437 30.0 22.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1865105100412876 1101 2 0.11248736489490696 1101 3 0.18838572613292115 1101 4 -0.030770511911271115 1101 5 0.30360293022732776 1101 6 0.3080360322146518 1101 7 0.4073187224249466 1101 8 0.385715624114475 1101 9 0.4133531780767967 1101 10-11 0.5005310575969233 1101 12-13 0.38500214742688144 1101 14-15 0.6005727613315983 1101 16-17 0.5413512169434966 1101 18-19 0.5185689786367504 1101 20-21 0.6527830869945319 1101 22-23 0.5010456134272303 1101 24-25 0.5490486231947003 1101 26-27 0.5648095744911998 1101 28-29 0.5015949719687001 1101 30-31 0.6530041781286329 1101 32-33 0.6491915099811223 1101 34-35 0.4428676258505675 1101 36-37 0.38975288503981176 1101 38-39 0.4968280863427381 1101 40-41 0.3619044039233046 1101 42-43 0.5205650915351256 1101 44-45 0.5560422016185527 1101 46-47 0.6787384121159192 1101 48-49 0.7577222004174047 1101 50-51 0.8563206254955773 1101 52-53 0.8618311069560392 1101 54-55 0.806243959863977 1101 56-57 0.8368734517604892 1101 58-59 0.8542630740288146 1101 60-61 0.6470688390843335 1101 62-63 0.618332800019644 1101 64-65 0.6213550513722907 1101 66-67 0.8237639622188837 1101 68-69 0.8763669584013485 1101 70-71 0.7688918571972465 1101 72-73 0.8511293459504969 1101 74-75 0.7134833212244942 1101 76-77 0.5758927888757697 1101 78-79 0.6359005943728526 1101 80-81 0.694218266924306 1101 82-83 0.7643143698429107 1101 84-85 0.7743489003496222 1101 86-87 0.6538175946403442 1101 88-89 0.5888372825851533 1101 90-91 0.6108916966285847 1101 92-93 0.7461416092272408 1101 94-95 1.0322216906298074 1101 96-97 1.0658538374660438 1101 98-99 1.0422116035970816 1101 100-101 0.9744754682330914 1102 1 0.36774727870184876 1102 2 0.3165848153654629 1102 3 0.25973906832423665 1102 4 0.30205634852895713 1102 5 0.37229623016311564 1102 6 0.3172743944635599 1102 7 0.21855903003835664 1102 8 0.3112964256131221 1102 9 0.37138585935277035 1102 10-11 0.4519364139186166 1102 12-13 0.5125575574686039 1102 14-15 0.5750451500823957 1102 16-17 0.5304029788969942 1102 18-19 0.5332026546339534 1102 20-21 0.5099937564075887 1102 22-23 0.5801250512635008 1102 24-25 0.6536040184024898 1102 26-27 0.61980532562864 1102 28-29 0.5737975568876763 1102 30-31 0.5933966628337899 1102 32-33 0.34671514361685496 1102 34-35 0.4151737304382763 1102 36-37 0.44366602387907506 1102 38-39 0.42538759478853905 1102 40-41 0.41332252802399694 1102 42-43 0.4661491882862805 1102 44-45 0.40287520966490575 1102 46-47 0.47931471045600915 1102 48-49 0.4951406402689784 1102 50-51 0.3567391543507554 1102 52-53 0.44562111354654377 1102 54-55 0.5167054329583962 1102 56-57 0.5750319989673613 1102 58-59 0.5259346041067374 1102 60-61 0.5956112461232479 1102 62-63 0.6655558345763595 1102 64-65 0.45065911735363073 1102 66-67 0.6092862014648048 1102 68-69 0.5724474621617546 1102 70-71 0.6147672818186152 1102 72-73 0.6045705537717936 1102 74-75 0.6008293926689632 1102 76-77 0.40736658261203473 1102 78-79 0.5248365456836375 1102 80-81 0.5457878031095689 1102 82-83 0.5679639414600857 1102 84-85 0.6598478821060034 1102 86-87 0.6746929133050372 1102 88-89 0.6337719179140713 1102 90-91 0.7157167515671183 1102 92-93 0.6984544844463088 1102 94-95 0.5141465419196187 1102 96-97 0.6412149907832294 1102 98-99 0.81241330271828 1102 100-101 0.6315562347660411 1103 1 0.26160052375023213 1103 2 0.21882142583255515 1103 3 0.2973530588854203 1103 4 0.2256303762031706 1103 5 0.28678137440321194 1103 6 0.1621872802277906 1103 7 0.24070514276788657 1103 8 0.21210939232409487 1103 9 0.16413621146852364 1103 10-11 0.21327217411042199 1103 12-13 0.26424403370202043 1103 14-15 0.37598036026860626 1103 16-17 0.43739553210545523 1103 18-19 0.30257120829114825 1103 20-21 0.47506495403342797 1103 22-23 0.44158699162844783 1103 24-25 0.3826372425889488 1103 26-27 0.47819265347529694 1103 28-29 0.35423402089931244 1103 30-31 0.4364388451950134 1103 32-33 0.38093855662586407 1103 34-35 0.3327394051085264 1103 36-37 0.36039225561525257 1103 38-39 0.35295955788341615 1103 40-41 0.261427869942942 1103 42-43 0.21185046856218293 1103 44-45 0.30131316396113306 1103 46-47 0.3968947939551697 1103 48-49 0.49443756660847527 1103 50-51 0.5700098916145109 1103 52-53 0.5460914331601003 1103 54-55 0.5454294333844842 1103 56-57 0.6658446723557532 1103 58-59 0.5754782044511089 1103 60-61 0.5553200436910473 1103 62-63 0.5682986838090613 1103 64-65 0.48555652906481583 1103 66-67 0.5117157873190408 1103 68-69 0.5890721631142313 1103 70-71 0.5482216493147618 1103 72-73 0.7057907757397999 1103 74-75 0.6975966432534193 1103 76-77 0.5746561738284086 1103 78-79 0.5356539739855712 1103 80-81 0.6116187834296802 1103 82-83 0.6400829604452625 1103 84-85 0.6615148035001823 1103 86-87 0.6386101181157287 1103 88-89 0.49472260215333463 1103 90-91 0.543274460213393 1103 92-93 0.610571048784557 1103 94-95 0.662525317097117 1103 96-97 0.7762056482014863 1103 98-99 0.840123410460297 1103 100-101 0.9421806104906629 1104 1 0.0552594545159657 1104 2 0.08505778016202825 1104 3 0.052546653869313786 1104 4 0.03936953675235344 1104 5 0.12778681368482125 1104 6 0.0555137176274485 1104 7 0.1204660331028009 1104 8 0.13765153574432532 1104 9 0.1661632210484072 1104 10-11 0.058676429235667626 1104 12-13 0.2513194743050917 1104 14-15 0.23799903093309638 1104 16-17 0.2762634597862501 1104 18-19 0.21766920193898898 1104 20-21 0.2878293424598084 1104 22-23 0.3600391583034721 1104 24-25 0.3319669396083569 1104 26-27 0.22115242162610826 1104 28-29 0.2891654185106489 1104 30-31 0.2479060690045145 1104 32-33 0.43777760644518793 1104 34-35 0.32490211572689986 1104 36-37 0.2810533235128503 1104 38-39 0.3315320388550447 1104 40-41 0.3304669504446096 1104 42-43 0.39256114465228364 1104 44-45 0.3882410406970749 1104 46-47 0.43950946163648297 1104 48-49 0.40727919252989153 1104 50-51 0.4844942383007478 1104 52-53 0.4369063229087047 1104 54-55 0.44995568729763136 1104 56-57 0.366364829291399 1104 58-59 0.4532306920756426 1104 60-61 0.5005752160210335 1104 62-63 0.5765582352088288 1104 64-65 0.5754850483073639 1104 66-67 0.5792378383580612 1104 68-69 0.5153999721059748 1104 70-71 0.4089264471530214 1104 72-73 0.4165898990144896 1104 74-75 0.4364579635968724 1104 76-77 0.5879038851834579 1104 78-79 0.4692331458355703 1104 80-81 0.5006865261050315 1104 82-83 0.5578895826680927 1104 84-85 0.37723525389274926 1104 86-87 0.37723790326030837 1104 88-89 0.35475147697695064 1104 90-91 0.5488831023068421 1104 92-93 0.2940507308425211 1104 94-95 0.06581554325032712 1104 96-97 0.47107235466407005 1104 98-99 0.3630850468926994 1104 100-101 0.530241607606218 1105 1 0.16434595631032778 1105 2 0.15261187211292437 1105 3 0.20296356441534869 1105 4 0.21719454665533533 1105 5 0.1199476932665462 1105 6 0.19528467795048954 1105 7 0.22092659559208272 1105 8 0.22728089822888364 1105 9 0.1402597834825059 1105 10-11 0.2710825479965351 1105 12-13 0.3194054203501011 1105 14-15 0.23723290484105775 1105 16-17 0.19032381968322198 1105 18-19 0.20404644538282923 1105 20-21 0.3051768020743637 1105 22-23 0.354905620801226 1105 24-25 0.23264004666104654 1105 26-27 0.21628862948424654 1105 28-29 0.23892799332494974 1105 30-31 0.32219147782516444 1105 32-33 0.20635776540526507 1105 34-35 0.05339614339218457 1105 36-37 0.19612147178362704 1105 38-39 0.2405211859768741 1105 40-41 0.20783381201768947 1105 42-43 0.2735714161614595 1105 44-45 0.14734513136698268 1105 46-47 0.10999000745328402 1105 48-49 0.18928577283863035 1105 50-51 0.10464374866980819 1105 52-53 0.13045046655216908 1105 54-55 0.3261041374072988 1105 56-57 0.2615203504246537 1105 58-59 0.3113874961621761 1105 60-61 0.314579261955096 1105 62-63 0.23803273478807085 1105 64-65 0.26362369200305125 1105 66-67 0.20652439218002172 1105 68-69 0.4422543913971779 1105 70-71 0.38542238840913967 1105 72-73 0.34512733228022796 1105 74-75 0.3710601101294557 1105 76-77 0.4539700173847976 1105 78-79 0.4161411003429514 1105 80-81 0.3475337324791994 1105 82-83 0.317754626231352 1105 84-85 0.3347625536012764 1105 86-87 0.3453431625804413 1105 88-89 0.2604672210405816 1105 90-91 0.24302462722786444 1105 92-93 0.11350880625494852 1105 94-95 0.06200486655652426 1105 96-97 0.25569189299796236 1105 98-99 0.391150881511944 1105 100-101 0.5321345056419808 1106 1 0.19464476708859024 1106 2 0.18974588535995096 1106 3 0.19797523083111912 1106 4 0.24809455418205317 1106 5 0.2599305699788772 1106 6 0.28906008063000144 1106 7 0.18739317695592206 1106 8 0.20513352505411575 1106 9 0.2746597157420325 1106 10-11 0.2911520760712065 1106 12-13 0.3345651749790406 1106 14-15 0.30639922653306684 1106 16-17 0.34344599716327195 1106 18-19 0.2811769775219233 1106 20-21 0.21205904654222962 1106 22-23 0.2527750886621405 1106 24-25 0.29969512343358673 1106 26-27 0.19259216327922246 1106 28-29 0.2888635457861852 1106 30-31 0.20090591407385006 1106 32-33 0.18505808905421617 1106 34-35 0.07471087318294423 1106 36-37 0.2342076150923731 1106 38-39 0.2737944623580262 1106 40-41 0.26508862911840225 1106 42-43 0.27529315332839843 1106 44-45 0.40834402117580737 1106 46-47 0.39039184020956696 1106 48-49 0.33526838611576437 1106 50-51 0.2951544366120018 1106 52-53 0.2751921118931264 1106 54-55 0.31058780442521083 1106 56-57 0.341933473260724 1106 58-59 0.4449980636768487 1106 60-61 0.5786876279042161 1106 62-63 0.49622886229070673 1106 64-65 0.3863703802463121 1106 66-67 0.4391212046141604 1106 68-69 0.4612645336521837 1106 70-71 0.47366471115488196 1106 72-73 0.5464346294351259 1106 74-75 0.4723641143444155 1106 76-77 0.5444148465282623 1106 78-79 0.673710157997629 1106 80-81 0.502218645169247 1106 82-83 0.6025798181627096 1106 84-85 0.6009708743900788 1106 86-87 0.5348048139427775 1106 88-89 0.3963927253308128 1106 90-91 0.47770916358192395 1106 92-93 0.49437729564227695 1106 94-95 0.6049885523912337 1106 96-97 0.5286126366378845 1106 98-99 0.7129976563124849 1106 100-101 0.8853037251211298 1107 1 -0.14725785993673668 1107 2 -0.12177192964712447 1107 3 -0.057984734561358664 1107 4 1.4249346158834442E-4 1107 5 -0.08203006396795587 1107 6 -0.013370311064193174 1107 7 -0.13697562908465954 1107 8 -0.013149470069372171 1107 9 -0.06884842489034781 1107 10-11 -0.019498093033725183 1107 12-13 2.614546707135901E-4 1107 14-15 0.13449768099271608 1107 16-17 -0.1659231038277582 1107 18-19 0.04732109031097309 1107 20-21 0.07636905912678316 1107 22-23 0.0775818961184811 1107 24-25 0.06901585255109666 1107 26-27 0.01740486974453148 1107 28-29 0.13900463041730404 1107 30-31 0.13189537451115996 1107 32-33 -0.049426534195738725 1107 34-35 -0.09707318376216278 1107 36-37 0.13736653439393365 1107 38-39 0.026985334624818336 1107 40-41 0.10944819337827028 1107 42-43 -0.2422367349836989 1107 44-45 0.003013774990499485 1107 46-47 0.12335697706657811 1107 48-49 0.024569550186868128 1107 50-51 -0.07680757377026026 1107 52-53 -0.15853940454544357 1107 54-55 -0.09885454259382698 1107 56-57 -0.11582028244165343 1107 58-59 0.06544796142735976 1107 60-61 0.09547571583878067 1107 62-63 0.09970878599686017 1107 64-65 -0.04456697227846007 1107 66-67 0.01048200766592089 1107 68-69 0.1366474776509321 1107 70-71 0.15147165625477044 1107 72-73 0.029214930677852635 1107 74-75 0.09031873130209789 1107 76-77 0.060806226109825445 1107 78-79 0.10133423381956064 1107 80-81 0.13415145545712193 1107 82-83 0.31462461130412933 1107 84-85 0.27175455773127766 1107 86-87 0.06246625015482721 1107 88-89 0.3585727040943816 1107 90-91 0.43873785242886143 1107 92-93 -0.077160477678472 1107 94-95 0.011849591403347404 1107 96-97 0.18753390453154495 1107 98-99 0.30950986726104546 1107 100-101 0.2684999064959932 1108 1 0.08614152334438074 1108 2 0.05781238211620732 1108 3 0.03651786289785264 1108 4 0.03308644482153511 1108 5 0.08983673309287354 1108 6 0.00745674420738851 1108 7 0.044587338216352634 1108 8 0.11720488742668067 1108 9 0.10446161564934897 1108 10-11 0.04492003251235843 1108 12-13 -0.04199298072068558 1108 14-15 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-1.6891558006705658 2316 80-81 -1.4364258726263905 2316 82-83 -1.5645054286800004 2316 84-85 -1.6788188787672134 2316 86-87 -1.8281329013270238 2316 88-89 -1.5933353171490943 2316 90-91 -1.8377436205742192 2316 92-93 -2.0300307123944634 2316 94-95 -1.8979427047844233 2316 96-97 -2.154036617093457 2316 98-99 -1.902892843113463 2316 100-101 -1.7865999775195114 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16827.0 3 3674.0 4 3051.0 5 2673.0 6 2637.0 7 2752.0 8 3214.0 9 3885.0 10 4594.0 11 5480.0 12 7294.0 13 10449.0 14 10559.0 15 9163.0 16 10581.0 17 12799.0 18 11996.0 19 9470.0 20 9270.0 21 9697.0 22 10206.0 23 11360.0 24 13331.0 25 15572.0 26 18359.0 27 22056.0 28 27072.0 29 32981.0 30 40516.0 31 51167.0 32 65518.0 33 86049.0 34 122811.0 35 184192.0 36 310198.0 37 497456.0 38 458980.0 39 82487.0 40 46.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.67633654146934 17.430690440336672 12.506138015320486 24.3868350028735 2 16.060028416141023 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24.393086596606107 25.293926900659873 27.05674978054473 23.256236722189286 22-23 23.861295147661778 25.215537517776816 26.98515178413569 23.93801555042571 24-25 23.74936674855469 25.2621700757129 27.326575469367448 23.661887706364972 26-27 23.628270379802753 25.685776752343642 26.966784160330104 23.719168707523494 28-29 23.385563066584726 25.937057959367714 27.160021890670283 23.51735708337727 30-31 23.643311227605366 25.576738601755324 27.041302735751444 23.73864743488787 32-33 23.44242545997568 25.488587448955037 27.214474874025264 23.854512217044025 34-35 23.89074470685607 25.77389811456843 26.924143279221614 23.41121389935389 36-37 23.971035558889177 25.566896555502243 26.75451351051528 23.707554375093302 38-39 23.65940478350732 25.708801110224726 26.94050231734304 23.691291788924914 40-41 23.88996901474562 25.5585199404221 27.040364846119353 23.51114619871292 42-43 23.813816542193724 25.577020885704343 26.75962036285407 23.849542209247858 44-45 23.719760306233763 25.726028911017135 26.83358288115633 23.720627901592767 46-47 23.92318529190822 26.051081940711885 26.409651043581306 23.616081723798583 48-49 24.159932555754647 25.9310747031332 26.3883602046984 23.52063253641375 50-51 23.858616889259153 26.006667497934394 26.35344772856094 23.781267884245512 52-53 24.076113526358803 25.942485111385178 26.331560849285275 23.64984051297075 54-55 23.95725858982388 25.90021600846225 26.29337378459566 23.84915161711821 56-57 23.784011592267955 26.26410307748962 26.200589430388515 23.751295899853908 58-59 23.932506289580985 25.917593932780246 26.364005789610662 23.78589398802811 60-61 23.861145665373552 25.945572413774208 26.485090707697424 23.708191213154812 62-63 23.83987132141863 25.95034798324494 26.562991165218246 23.646789530118177 64-65 23.673046855021955 26.056986972944934 26.441866858090584 23.82809931394253 66-67 23.735977433328753 26.29645827617329 26.1829447059033 23.784619584594658 68-69 23.713232691915152 25.93549347523411 26.522121917706254 23.829151915144482 70-71 23.692167282603908 25.737943339557006 26.4333067691613 24.136582608677788 72-73 23.77305361552286 26.09957846904633 26.240050737131256 23.887317178299558 74-75 23.6542562312667 26.322488045051028 26.071298829313495 23.95195689436878 76-77 23.881649931349507 26.172640347676957 26.063603185090585 23.88210653588295 78-79 23.857953198991268 26.278160488128066 26.103075639668123 23.760810673212543 80-81 23.83083366285818 26.35510054236898 25.99261559929535 23.82145019547749 82-83 23.95038965273031 26.455564246468192 26.000250225225535 23.593795875575964 84-85 23.95041880054647 26.302655995441228 25.776712461370995 23.970212742641312 86-87 23.829209561345852 26.142400098995182 26.19171504363802 23.836675296020946 88-89 23.9262227702425 26.3270767685071 25.873863999634654 23.872836461615744 90-91 23.833553701604533 26.468357301285415 25.985907241592983 23.71218175551707 92-93 23.79907971712343 26.246719759499655 26.064435330476737 23.889765192900178 94-95 23.884389917484317 26.086626970800232 26.253859182943362 23.77512392877209 96-97 23.96993777892609 25.9939657358422 26.120023837603018 23.916072647628692 98-99 23.55292655423449 26.117374375331348 26.041011720731778 24.28868734970239 100-101 23.748044256018442 26.14060814936121 25.878792374452935 24.232555220167416 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 70.0 1 51.0 2 90.5 3 181.0 4 162.0 5 140.0 6 288.0 7 549.5 8 854.5 9 994.0 10 944.5 11 976.0 12 1120.5 13 1330.0 14 1548.0 15 1736.5 16 1908.0 17 2031.5 18 2173.0 19 2356.0 20 2455.5 21 2581.0 22 2865.5 23 3299.5 24 4046.0 25 5059.0 26 6836.5 27 9325.0 28 11347.0 29 13798.5 30 17017.0 31 20656.5 32 25182.0 33 28794.5 34 31963.0 35 36053.5 36 41159.0 37 45868.0 38 49037.5 39 51747.0 40 54876.0 41 58180.0 42 61037.0 43 64677.5 44 68124.0 45 71726.0 46 75549.0 47 78926.5 48 82885.0 49 84252.0 50 82662.0 51 81583.5 52 83304.0 53 85819.5 54 87996.5 55 86022.0 56 79403.5 57 73196.5 58 69218.0 59 64419.0 60 56842.5 61 48845.5 62 40864.5 63 32291.5 64 24159.0 65 18143.5 66 13388.5 67 10901.0 68 8327.5 69 6036.5 70 4845.0 71 3318.5 72 2511.5 73 2158.0 74 1654.5 75 1197.0 76 865.0 77 500.5 78 297.0 79 203.0 80 127.5 81 81.0 82 61.0 83 41.5 84 29.0 85 29.5 86 29.0 87 21.5 88 20.5 89 21.5 90 18.5 91 17.0 92 15.5 93 16.5 94 18.5 95 20.0 96 25.0 97 23.0 98 14.5 99 10.5 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0460820697120825 2 0.011997698623264084 3 0.010134419670408675 4 0.005271716061737249 5 0.007816682436369024 6 0.005907957655395192 7 0.0058170659991583435 8 0.009770853045461278 9 0.01495167745096168 10-11 0.00229501431998044 12-13 0.009089165623684912 14-15 0.00897555105338885 16-17 0.018882741583205402 18-19 0.01177046948267196 20-21 0.003976509960362149 22-23 0.010225311326645526 24-25 0.021200478817245055 26-27 0.007339501241125566 28-29 0.018087439591132973 30-31 0.014247267115126099 32-33 0.012656663130981238 34-35 0.2577914600017633 36-37 0.45186786898149534 38-39 0.44875482975538333 40-41 0.4702734293694573 42-43 0.4599117805584565 44-45 0.47540880794683926 46-47 0.4820438988521292 48-49 0.4890652792964259 50-51 0.49967688016207795 52-53 0.4883381460465311 54-55 0.4754315308608985 56-57 0.46997803148668754 58-59 0.4677284629948255 60-61 0.46479720708118716 62-63 0.4616614449410158 64-65 0.4779992201495895 66-67 0.45150430235654804 68-69 0.4787717992276027 70-71 0.4602980700974631 72-73 0.4685464879009572 74-75 0.48347544243785967 76-77 0.4700462002288652 78-79 0.46995530857262835 80-81 0.47263661243161537 82-83 0.4724093832910232 84-85 0.47072788765064155 86-87 0.47338646859556943 88-89 0.48727016908574805 90-91 0.5127880015742434 92-93 0.49929059062307146 94-95 0.47052338142410866 96-97 0.44380123449047504 98-99 0.43448483972619795 100-101 0.4435285595217645 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2200422.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.46186307800015 #Duplication Level Percentage of deduplicated Percentage of total 1 71.00093238735307 33.69836531378898 2 14.520029907290466 13.782953426965744 3 5.719219948321806 8.143345023006498 4 2.729820685891686 5.182495024851345 5 1.6060494111083248 3.8113048623263044 6 0.9795252565225435 2.7894056163909555 7 0.6696377958359175 2.22475801644727 8 0.4579893250616375 1.7389621309808911 9 0.35310878275639535 1.5083280628941003 >10 1.7571355299753795 15.142713254998933 >50 0.13427307408921926 4.405843013987731 >100 0.06824325565427894 5.7581925763048005 >500 0.0029779486741870853 1.0012271836508495 >1k 0.0010566914650341108 0.81210649340568 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3533 0.16056011074239396 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2546 0.11570507838950891 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.8178331247369823E-4 2 0.0 0.0 0.0 0.0 1.8178331247369823E-4 3 0.0 0.0 0.0 0.0 2.2722914059212279E-4 4 0.0 0.0 0.0 0.0 2.2722914059212279E-4 5 0.0 0.0 0.0 0.0 2.2722914059212279E-4 6 0.0 0.0 0.0 0.0 2.2722914059212279E-4 7 0.0 0.0 0.0 0.0 2.2722914059212279E-4 8 0.0 0.0 0.0 0.0 2.2722914059212279E-4 9 0.0 0.0 0.0 0.0 2.2722914059212279E-4 10-11 0.0 0.0 0.0 0.0 2.2722914059212279E-4 12-13 4.544582811842456E-5 0.0 0.0 0.0 9.316394764277034E-4 14-15 4.544582811842456E-5 0.0 0.0 0.0 0.0011815915310790384 16-17 4.544582811842456E-5 0.0 0.0 0.0 0.001386097757611949 18-19 4.544582811842456E-5 0.0 0.0 2.272291405921228E-5 0.0015224352419672227 20-21 4.544582811842456E-5 0.0 0.0 4.544582811842456E-5 0.0017042185544409209 22-23 4.544582811842456E-5 0.0 0.0 9.089165623684912E-5 0.001886001866914619 24-25 4.544582811842456E-5 0.0 0.0 2.2722914059212279E-4 0.001954170609092256 26-27 4.544582811842456E-5 0.0 0.0 3.408437108881842E-4 0.0020677851793883175 28-29 4.544582811842456E-5 0.0 0.0 7.044103358355806E-4 0.002135953921565954 30-31 4.544582811842456E-5 0.0 0.0 0.002363183062158077 0.002135953921565954 32-33 4.544582811842456E-5 0.0 0.0 0.0054534993742109475 0.002181399749684379 34-35 4.544582811842456E-5 0.0 0.0 0.008998273967448062 0.0022268455778028033 36-37 4.544582811842456E-5 0.0 0.0 0.015951485669567018 0.0022495684918620155 38-39 4.544582811842456E-5 0.0 0.0 0.02704026773046261 0.0022722914059212277 40-41 4.544582811842456E-5 0.0 0.0 0.04049223285351628 0.0022722914059212277 42-43 4.544582811842456E-5 0.0 0.0 0.05539846447635954 0.0022722914059212277 44-45 4.544582811842456E-5 0.0 0.0 0.0706682627241502 0.0024313518043357135 46-47 4.544582811842456E-5 0.0 0.0 0.09052808961190173 0.002613135116809412 48-49 4.544582811842456E-5 0.0 0.0 0.11316011201487715 0.0027267496871054737 50-51 4.544582811842456E-5 0.0 0.0 0.14072300676870164 0.0029085329995791717 52-53 4.544582811842456E-5 0.0 0.0 0.16742243078827607 0.003113039226112082 54-55 4.544582811842456E-5 0.0 0.0 0.19503077137021899 0.003203930882348931 56-57 4.544582811842456E-5 0.0 0.0 0.2280244425841952 0.003272099624526568 58-59 4.544582811842456E-5 0.0 0.0 0.2651082383288297 0.0033175454526449925 60-61 4.544582811842456E-5 4.544582811842456E-5 0.0 0.3057595315807604 0.0034084371088818417 62-63 4.544582811842456E-5 4.544582811842456E-5 0.0 0.3496602015431586 0.0036356662494739646 64-65 4.544582811842456E-5 4.544582811842456E-5 0.0 0.3958558858255371 0.003772003733829238 66-67 4.544582811842456E-5 4.544582811842456E-5 0.0 0.45145885652842954 0.0037947266478884504 68-69 4.544582811842456E-5 4.544582811842456E-5 0.0 0.5165600053080728 0.0038174495619476626 70-71 4.544582811842456E-5 4.544582811842456E-5 0.0 0.589705065664677 0.0038856183041253 72-73 4.544582811842456E-5 4.544582811842456E-5 0.0 0.664418007091367 0.004271907843131908 74-75 4.544582811842456E-5 4.544582811842456E-5 0.0 0.7376085132760898 0.004544582811842456 76-77 4.544582811842456E-5 4.544582811842456E-5 0.0 0.8247963345212873 0.004658197382138517 78-79 4.544582811842456E-5 4.544582811842456E-5 0.0 0.9290717871390124 0.004680920296197729 80-81 4.544582811842456E-5 4.544582811842456E-5 0.0 1.0452994925518833 0.004680920296197729 82-83 4.544582811842456E-5 4.544582811842456E-5 0.0 1.1646402371908662 0.004771811952434578 84-85 4.544582811842456E-5 4.544582811842456E-5 0.0 1.287503033509027 0.0048172577805530034 86-87 4.544582811842456E-5 4.544582811842456E-5 0.0 1.4171145353027739 0.004930872350849064 88-89 4.544582811842456E-5 4.544582811842456E-5 0.0 1.563041089391035 0.005067209835204338 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5230 0.0 42.46428 1 GGTATCA 2935 0.0 42.432663 1 TCAACGC 6385 0.0 34.475174 4 ATCAACG 6505 0.0 33.76719 3 CAACGCA 6625 0.0 33.08335 5 TATCAAC 6690 0.0 32.83342 2 AACGCAG 6820 0.0 32.13596 6 TACATGG 8840 0.0 27.257736 2 TACCTGG 1900 0.0 26.410496 2 GTACATG 9365 0.0 26.394617 1 ACGCAGA 8290 0.0 26.381042 7 CGCAGAG 8415 0.0 25.932913 8 ACATGGG 9095 0.0 25.660698 3 GTACCTG 2170 0.0 24.222326 1 GTGTACG 265 3.274181E-11 23.230045 1 GCAGAGT 9865 0.0 21.737303 9 GTACGCT 200 5.569018E-7 21.302805 3 CATGGGG 5210 0.0 21.17057 4 GAGTACA 7050 0.0 20.822132 1 AGTACAT 6345 0.0 20.74137 2 >>END_MODULE