FastQCFastQC Report
Thu 26 May 2016
SRR936299_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936299_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2200422
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40050.18201054161429037No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30010.1363829301833921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32450.044.4154931
GTATCAA53050.036.941831
TCAACGC65550.029.2381044
ATCAACG65600.029.2158163
TACATGG85550.028.9547182
CAACGCA67400.028.3651895
TATCAAC68650.028.3408012
GTACATG90000.028.011731
AACGCAG69650.027.516986
ACATGGG87500.027.1083133
TACCTGG19200.026.198322
GTACCTG20600.025.39991
CATGGGG50750.024.0235714
GTGTAGC13150.023.150521
ACGCAGA84750.022.5022967
CGCAGAG86550.021.9246888
AGCGTAC1456.090319E-419.6829321
AGTACAT58400.019.6639772
GAGTACA68000.019.5164971
GTAGCCC13450.019.399073
Produced by FastQC (version 0.11.5)