##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936299_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2200422 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.426099175521784 34.0 31.0 34.0 31.0 34.0 2 32.560179820052696 34.0 31.0 34.0 31.0 34.0 3 32.645364843652715 34.0 31.0 34.0 31.0 34.0 4 36.146546889642075 37.0 35.0 37.0 35.0 37.0 5 36.105447046066615 37.0 35.0 37.0 35.0 37.0 6 36.142125465024435 37.0 36.0 37.0 35.0 37.0 7 36.087662275690754 37.0 36.0 37.0 35.0 37.0 8 36.06456806921582 37.0 35.0 37.0 35.0 37.0 9 37.82621333544202 39.0 38.0 39.0 35.0 39.0 10-11 37.75778623373153 39.0 37.5 39.0 35.0 39.0 12-13 37.69535502735384 39.0 37.0 39.0 35.0 39.0 14-15 39.02924007304054 40.0 38.0 41.0 35.5 41.0 16-17 38.943551509664964 40.0 38.0 41.0 35.0 41.0 18-19 38.953073092343196 40.0 38.0 41.0 35.0 41.0 20-21 38.90174225671258 40.0 38.0 41.0 35.0 41.0 22-23 38.78104813531223 40.0 38.0 41.0 34.5 41.0 24-25 38.94107880215704 40.0 38.0 41.0 35.0 41.0 26-27 38.85505939315277 40.0 38.0 41.0 35.0 41.0 28-29 38.76587490944919 40.0 38.0 41.0 35.0 41.0 30-31 38.65368665646862 40.0 38.0 41.0 34.0 41.0 32-33 38.49932967403525 40.0 38.0 41.0 34.0 41.0 34-35 38.3545406290248 40.0 38.0 41.0 34.0 41.0 36-37 38.20641995035498 40.0 38.0 41.0 33.5 41.0 38-39 38.06339238564239 40.0 38.0 41.0 33.0 41.0 40-41 37.90913197559377 40.0 37.0 41.0 33.0 41.0 42-43 37.94089974559425 40.0 37.5 41.0 33.0 41.0 44-45 37.81352167902338 40.0 37.0 41.0 33.0 41.0 46-47 37.61934392584695 40.0 37.0 41.0 33.0 41.0 48-49 37.377240820169945 40.0 37.0 41.0 32.0 41.0 50-51 37.16362656799468 40.0 36.0 41.0 31.5 41.0 52-53 36.93347639680025 39.5 36.0 41.0 31.0 41.0 54-55 36.723022674741486 39.0 35.5 41.0 31.0 41.0 56-57 36.44552794872983 39.0 35.0 41.0 31.0 41.0 58-59 36.16361066195485 38.5 35.0 40.5 30.0 41.0 60-61 35.87217860937584 38.0 35.0 40.0 30.0 41.0 62-63 35.555500035902206 37.5 35.0 40.0 29.0 41.0 64-65 35.1637722218738 37.0 34.0 40.0 29.0 41.0 66-67 34.80746397736434 36.5 34.0 39.0 28.0 41.0 68-69 34.44579766971972 36.0 34.0 39.0 28.0 41.0 70-71 34.06826531456239 35.5 34.0 39.0 28.0 40.0 72-73 33.6646913637475 35.0 33.5 37.5 27.0 40.0 74-75 33.23477292083064 35.0 33.0 37.0 26.0 39.0 76-77 32.53142874412272 35.0 32.0 36.0 26.0 39.0 78-79 32.60965237577156 35.0 33.0 36.0 26.0 38.5 80-81 32.458907200527904 35.0 33.0 36.0 26.0 37.0 82-83 32.17585263190424 35.0 33.0 35.0 26.0 37.0 84-85 31.88689624081199 35.0 32.5 35.0 25.5 36.5 86-87 31.479221940155114 35.0 32.0 35.0 24.5 36.0 88-89 30.981249960234898 34.0 31.5 35.0 22.0 36.0 90-91 30.75407194619941 34.0 31.0 35.0 20.0 35.5 92-93 30.451614054031452 34.0 31.0 35.0 19.5 35.0 94-95 30.26646411461074 34.0 31.0 35.0 18.0 35.0 96-97 29.986939096227907 34.0 31.0 35.0 12.0 35.0 98-99 29.485731146116517 34.0 31.0 35.0 2.0 35.0 100-101 25.99018029268931 30.5 24.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13987477385666836 1101 2 0.15641298209430232 1101 3 0.2248415748692878 1101 4 0.17225406667731846 1101 5 0.1386992322774816 1101 6 0.1687117128135398 1101 7 0.14101057419762952 1101 8 0.0917506045997456 1101 9 0.1669185919180194 1101 10-11 0.1664738170328377 1101 12-13 0.2335775922264105 1101 14-15 0.3666952417953837 1101 16-17 0.3613388034567251 1101 18-19 0.32620104301324915 1101 20-21 0.2727339666267028 1101 22-23 0.3118162970169891 1101 24-25 0.2065579797922723 1101 26-27 0.26109707953221317 1101 28-29 0.3148668054235344 1101 30-31 0.27085293879300565 1101 32-33 0.30626976795839767 1101 34-35 0.27663589599603 1101 36-37 0.21391433335453058 1101 38-39 0.3142647417938065 1101 40-41 0.37128342080184495 1101 42-43 0.3752533250427561 1101 44-45 0.37598028801259176 1101 46-47 0.32525367166946495 1101 48-49 0.4098113557712324 1101 50-51 0.3098474792168915 1101 52-53 0.3410090301497348 1101 54-55 0.3595814023026733 1101 56-57 0.2947316477039905 1101 58-59 0.20524105711625396 1101 60-61 0.3582242982382553 1101 62-63 0.25839272851823836 1101 64-65 0.38733781437949233 1101 66-67 0.32033467864614096 1101 68-69 0.4365855997589776 1101 70-71 0.34984341570982025 1101 72-73 0.4207308849533433 1101 74-75 0.29668124462848766 1101 76-77 0.2101763084053374 1101 78-79 0.2237716156886691 1101 80-81 0.32759741160781175 1101 82-83 0.3099639974422814 1101 84-85 0.44537970804951854 1101 86-87 0.3221815287280698 1101 88-89 0.2648487318177537 1101 90-91 0.4781431103315832 1101 92-93 0.7276908237273858 1101 94-95 0.6047629453781589 1101 96-97 0.4242211212408442 1101 98-99 0.4251288780025 1101 100-101 0.7114189455325182 1102 1 0.13641846720958029 1102 2 0.1634792325631622 1102 3 0.16212317167872214 1102 4 0.180207704275837 1102 5 0.18034444271802386 1102 6 0.15219942501826011 1102 7 0.19249598078516783 1102 8 0.1767702383711054 1102 9 0.16017233059920244 1102 10-11 0.22622437383515148 1102 12-13 0.23025505587796857 1102 14-15 0.28146078087115 1102 16-17 0.30052920971579766 1102 18-19 0.4066814929476621 1102 20-21 0.28228437127523165 1102 22-23 0.26422771194119576 1102 24-25 0.13470823059171977 1102 26-27 0.22009979414215763 1102 28-29 0.24435926236803596 1102 30-31 0.31636954114397753 1102 32-33 0.12214091193020238 1102 34-35 0.14260944709152312 1102 36-37 0.16160167004881032 1102 38-39 0.2483965116304887 1102 40-41 0.17811460639342158 1102 42-43 0.07953752414805848 1102 44-45 0.16396787412887193 1102 46-47 0.13600631369908456 1102 48-49 0.21743589194589674 1102 50-51 0.05947451173074825 1102 52-53 0.09941960589710419 1102 54-55 0.2279021585790204 1102 56-57 0.3042303984748216 1102 58-59 0.16257972218933503 1102 60-61 0.20402343405562817 1102 62-63 0.2500041387803549 1102 64-65 0.3590892357882467 1102 66-67 0.320694136656563 1102 68-69 0.16030092808691876 1102 70-71 0.13563054663624285 1102 72-73 0.031697983287493514 1102 74-75 0.10474814990958237 1102 76-77 0.07654825658828912 1102 78-79 0.03693768581926804 1102 80-81 0.05915459324508987 1102 82-83 0.10550814852801693 1102 84-85 0.19612723237186813 1102 86-87 0.18812461728029817 1102 88-89 0.24808744584280973 1102 90-91 0.47300156281319516 1102 92-93 0.30113336660275536 1102 94-95 0.1777880189650638 1102 96-97 0.026983199771688504 1102 98-99 0.3061893347605249 1102 100-101 0.7291240405650541 1103 1 0.04030540318755271 1103 2 0.13330920323604545 1103 3 0.14758826551879167 1103 4 0.06952679962780195 1103 5 0.14444355899367167 1103 6 0.12455737326278182 1103 7 0.11034970338330652 1103 8 0.11033730349335258 1103 9 0.2576817645226299 1103 10-11 0.21586815670850257 1103 12-13 0.17782647349574177 1103 14-15 0.23811889486866278 1103 16-17 0.36009672357014466 1103 18-19 0.23923983712621322 1103 20-21 0.27512678551568825 1103 22-23 0.27212477938429913 1103 24-25 0.2200873603293303 1103 26-27 0.29877714598465843 1103 28-29 0.38330025461145567 1103 30-31 0.3095326804778651 1103 32-33 0.344539256355759 1103 34-35 0.3574417411880617 1103 36-37 0.3495699891508508 1103 38-39 0.3847256463339974 1103 40-41 0.4436757556622055 1103 42-43 0.4635975255992335 1103 44-45 0.4613699622769474 1103 46-47 0.40270254419505846 1103 48-49 0.26693703770509813 1103 50-51 0.347862462244656 1103 52-53 0.26594282752598986 1103 54-55 0.37428338119902804 1103 56-57 0.3705566822886155 1103 58-59 0.3416153392705894 1103 60-61 0.3223546549517309 1103 62-63 0.25213548760626736 1103 64-65 0.24546043912615545 1103 66-67 0.3063380543554004 1103 68-69 0.29141136507067955 1103 70-71 0.31988087327924575 1103 72-73 0.21338122268040394 1103 74-75 0.4083037085527508 1103 76-77 0.41564216252848496 1103 78-79 0.36383310293685867 1103 80-81 0.2860021781967177 1103 82-83 0.4065889908064051 1103 84-85 0.42108147147506614 1103 86-87 0.3602433532349103 1103 88-89 0.38264802544824406 1103 90-91 0.41068903840180937 1103 92-93 0.4491905161350047 1103 94-95 0.5147082501444338 1103 96-97 0.32696884240288426 1103 98-99 0.4163476620601614 1103 100-101 0.8228833194649212 1104 1 0.047072987438696146 1104 2 0.07899840927712631 1104 3 0.016890576264145807 1104 4 -0.015211921951156171 1104 5 0.030538379600663745 1104 6 0.022773999753795238 1104 7 0.08751185959290808 1104 8 0.04215186059668952 1104 9 0.04131576967786543 1104 10-11 0.05800177985332766 1104 12-13 0.1399087113021693 1104 14-15 0.16154099436148783 1104 16-17 0.11966103422739849 1104 18-19 0.17914838246393572 1104 20-21 0.05463272376518091 1104 22-23 0.11941592297009151 1104 24-25 0.16171457283800095 1104 26-27 0.20010491700320898 1104 28-29 0.2045565904403901 1104 30-31 0.15016104585298962 1104 32-33 0.07394942618711298 1104 34-35 0.054926320270297424 1104 36-37 0.0636849325176172 1104 38-39 0.18052968334116315 1104 40-41 0.25166425433845774 1104 42-43 0.23717888470573456 1104 44-45 0.2561711269910347 1104 46-47 0.2749098049752732 1104 48-49 0.21461110307064502 1104 50-51 0.34897697575476627 1104 52-53 0.26253815013873094 1104 54-55 0.2544072953552856 1104 56-57 0.2127548598786504 1104 58-59 0.3150219456965715 1104 60-61 0.2139722982132355 1104 62-63 0.18519556577030727 1104 64-65 0.07445039749232762 1104 66-67 0.07319397946616135 1104 68-69 0.2525598418136141 1104 70-71 0.23543150750767694 1104 72-73 0.1241274872564162 1104 74-75 0.1769565256327894 1104 76-77 0.2071575059809163 1104 78-79 0.2236121140842755 1104 80-81 0.3498666503160379 1104 82-83 0.18421183791565454 1104 84-85 0.3148495372264257 1104 86-87 0.22712639121266776 1104 88-89 0.3369309470761124 1104 90-91 0.5376387725134428 1104 92-93 0.33078437298210517 1104 94-95 0.368433952990447 1104 96-97 0.2980662983501752 1104 98-99 0.248871786436073 1104 100-101 0.4261761277959373 1105 1 -0.0562737272352507 1105 2 0.07449772641490426 1105 3 0.09346377062072975 1105 4 0.06806081105464301 1105 5 0.005314848148600504 1105 6 0.04543113196893245 1105 7 0.052224732284926745 1105 8 0.0625382049287424 1105 9 0.0011027718437119916 1105 10-11 0.16152118082648315 1105 12-13 0.1678390573842421 1105 14-15 0.12446985253307474 1105 16-17 0.11829801954339558 1105 18-19 0.1601937824556643 1105 20-21 0.06501040668455715 1105 22-23 0.005472526632118502 1105 24-25 0.1381499137665756 1105 26-27 0.15061464951434544 1105 28-29 0.017868166778079342 1105 30-31 0.08018885302767131 1105 32-33 0.03542757163972965 1105 34-35 0.03434314804515992 1105 36-37 0.15252575171579963 1105 38-39 0.14309567857590366 1105 40-41 0.15118004294086518 1105 42-43 0.010842819923489344 1105 44-45 -0.019000426131313475 1105 46-47 0.061590493473822505 1105 48-49 0.10199278462626893 1105 50-51 0.04115367054392749 1105 52-53 -0.04009224895630581 1105 54-55 0.033866691165094664 1105 56-57 0.15565013660043547 1105 58-59 0.19146797790244108 1105 60-61 0.15464507023972374 1105 62-63 0.11667359297837265 1105 64-65 0.25466246907758006 1105 66-67 0.1951715997159411 1105 68-69 0.18651115068215063 1105 70-71 0.10655801340119098 1105 72-73 0.02684918099123479 1105 74-75 0.11574871676326381 1105 76-77 -0.013209919224486555 1105 78-79 -0.04535825186287923 1105 80-81 0.12831850409483536 1105 82-83 0.09637314120699614 1105 84-85 0.007548232397908805 1105 86-87 0.04727299473674762 1105 88-89 0.1785835229349111 1105 90-91 0.3371046520656087 1105 92-93 0.41608598524642915 1105 94-95 0.20315271820211578 1105 96-97 0.15443539277434937 1105 98-99 0.09832579226552696 1105 100-101 0.40537297145497675 1106 1 0.0067992443137470104 1106 2 0.04875352198919103 1106 3 0.028913680300092892 1106 4 0.04765022848652478 1106 5 0.030121035112323113 1106 6 0.11829166185000872 1106 7 0.12066895929079635 1106 8 0.12442101390058724 1106 9 0.005752403034442466 1106 10-11 0.11788078868437424 1106 12-13 0.11376004564255737 1106 14-15 0.1781217229229597 1106 16-17 0.07635960692861232 1106 18-19 0.12170590047462326 1106 20-21 0.14740399768260204 1106 22-23 0.1049710589217483 1106 24-25 0.22497203855959214 1106 26-27 0.1145042701676644 1106 28-29 0.1241059358583172 1106 30-31 0.08783364830089369 1106 32-33 0.09543952030749381 1106 34-35 0.19043885028811758 1106 36-37 0.22214414701912943 1106 38-39 0.30828648092424515 1106 40-41 0.16009732428965862 1106 42-43 0.10401984837449874 1106 44-45 0.11349571642476519 1106 46-47 0.17160808718148957 1106 48-49 0.17649743237578974 1106 50-51 0.28128849291333324 1106 52-53 0.15517579635454837 1106 54-55 0.18369451744840148 1106 56-57 0.3479432458884091 1106 58-59 0.37665021860694026 1106 60-61 0.22844596968275255 1106 62-63 0.20859130711355078 1106 64-65 0.36220094943283954 1106 66-67 0.2193502464117003 1106 68-69 0.11352360740351486 1106 70-71 0.16342191148940088 1106 72-73 0.03853676566683362 1106 74-75 0.2501227759232876 1106 76-77 0.2947444124027925 1106 78-79 0.1378040054004117 1106 80-81 0.14426009674875928 1106 82-83 0.14221933268675002 1106 84-85 0.28572450817692996 1106 86-87 0.21732605811173045 1106 88-89 0.2982353180575963 1106 90-91 0.44584646675776796 1106 92-93 0.5608201038675347 1106 94-95 0.512395530184655 1106 96-97 0.5066068514526592 1106 98-99 0.42367860725875417 1106 100-101 0.627659588947072 1107 1 -0.11008076984387571 1107 2 -0.00445848839306251 1107 3 0.019151803628709274 1107 4 0.0029613028926931406 1107 5 0.021042460312891365 1107 6 -4.730856724037835E-4 1107 7 -0.0736561940834406 1107 8 -0.056114222846304074 1107 9 -0.016209607347811072 1107 10-11 -0.016109792564641623 1107 12-13 0.053158051777529636 1107 14-15 0.023179828161275395 1107 16-17 0.009095665604085923 1107 18-19 0.019782823551551587 1107 20-21 -0.06250781213334733 1107 22-23 -0.11697423760176662 1107 24-25 0.06404854870589105 1107 26-27 0.05920990962023609 1107 28-29 0.023978123997380862 1107 30-31 -0.07243701789385426 1107 32-33 -0.006843887746612154 1107 34-35 -5.602451971711275E-4 1107 36-37 -0.0757597384614499 1107 38-39 0.04174762611852145 1107 40-41 0.042508445887889934 1107 42-43 -0.10840739718158687 1107 44-45 -0.08324852220981427 1107 46-47 -0.08490491030160285 1107 48-49 -0.237977260875752 1107 50-51 -0.05610090066854667 1107 52-53 0.03645479981530286 1107 54-55 0.0901254203900379 1107 56-57 -0.14418698456620405 1107 58-59 -0.19824114131125725 1107 60-61 -0.03946587945992519 1107 62-63 -0.10209228970884965 1107 64-65 0.030539493557427022 1107 66-67 -0.15954408267433706 1107 68-69 -0.052019298055036245 1107 70-71 0.08732420423755372 1107 72-73 0.14137861323644074 1107 74-75 0.14270526373199743 1107 76-77 -0.054478393165872774 1107 78-79 -0.1109970210271527 1107 80-81 0.02868278040457284 1107 82-83 0.20717626992129112 1107 84-85 0.04825793589440153 1107 86-87 -0.027319608083015368 1107 88-89 0.026188263629411068 1107 90-91 -0.14460549564588376 1107 92-93 0.009926404009110712 1107 94-95 0.11388601311685775 1107 96-97 -0.029066422104047973 1107 98-99 -0.04539977090704994 1107 100-101 0.23138776261389538 1108 1 -0.129826103230279 1108 2 -0.07544133435928302 1108 3 -0.030881268726787425 1108 4 -0.07224087438650173 1108 5 -0.015511495008155407 1108 6 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-1.1384422631800746 2316 84-85 -0.9797462151296195 2316 86-87 -1.101775549236148 2316 88-89 -0.9277082812535404 2316 90-91 -1.0106941474787448 2316 92-93 -0.9500223186353338 2316 94-95 -1.0303139990920407 2316 96-97 -1.0024201290295451 2316 98-99 -1.2350828737975057 2316 100-101 -1.1666572882056236 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 14.0 5 31.0 6 80.0 7 182.0 8 367.0 9 699.0 10 1164.0 11 1940.0 12 2790.0 13 4116.0 14 5362.0 15 6901.0 16 8713.0 17 11338.0 18 10667.0 19 7684.0 20 7317.0 21 7757.0 22 8302.0 23 9391.0 24 11374.0 25 14048.0 26 17213.0 27 21730.0 28 27232.0 29 34206.0 30 42209.0 31 52585.0 32 65902.0 33 84990.0 34 117838.0 35 178046.0 36 304868.0 37 530353.0 38 517404.0 39 85578.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.89395815004367 17.924988643824314 12.945037932132669 24.23601527399935 2 16.325537103493417 18.966406741833506 39.290180204955014 25.417875949718066 3 20.26365851641185 22.85902431442696 28.053891480815953 28.823425688345232 4 13.170791784484976 14.83829010980621 35.29186674192496 36.69905136378386 5 14.496128469902592 37.025079734705436 33.37059891239044 15.108192883001534 6 35.364443729430086 34.157947884542146 16.17008010281664 14.307528283211129 7 31.240780177620476 30.991009906281615 19.13464780846583 18.63356210763208 8 27.79762245605616 35.0633196723174 18.326848213660835 18.81220965796561 9 27.080487288347417 15.643044834127274 18.859882331661836 38.41658554586348 10-11 26.601348566245125 25.58076650225799 26.051564912191406 21.766320019305486 12-13 26.625330646878336 22.287093631833717 27.116327485438585 23.97124823584936 14-15 23.302757380175258 24.029413448874806 24.81794401255759 27.849885158392347 16-17 23.836229835035514 27.258201074071092 25.36989646943551 23.53567262145788 18-19 23.563275589864126 25.953862486377616 27.12191116067736 23.3609507630809 20-21 24.172533347027684 25.316246300139838 27.25844281617018 23.252777536662304 22-23 23.738139201543703 25.1594353989532 27.26616861046136 23.836256789041734 24-25 23.585303535866306 25.2790899332202 27.56286908632473 23.572737444588764 26-27 23.495358614121784 25.69774171711849 27.18574382949386 23.621155839265867 28-29 23.282430519056003 25.89621879416162 27.279486468068203 23.541864218714174 30-31 23.553236374940123 25.58105502923567 27.209536110351145 23.656172485473057 32-33 23.331445026994146 25.425488136278002 27.39164450576706 23.851422330960794 34-35 23.67224668642537 25.747910418220847 27.17020987960946 23.409633015744323 36-37 23.903118495559937 25.556416502304106 27.008639259777677 23.53182574235828 38-39 23.53220668504043 25.729968431033978 27.11468103482948 23.623143849096113 40-41 23.78777567336959 25.538837692563764 27.170649072955804 23.50273756111084 42-43 23.706631702373272 25.56298117563649 26.91006844396775 23.82031867802249 44-45 23.581624426109855 25.752044916817173 27.008672319747816 23.657658337325152 46-47 23.73755577544285 26.031274700390934 26.643287606977268 23.587881917188948 48-49 23.999643655152727 25.940859481954586 26.524692334404186 23.534804528488497 50-51 23.78600523795186 26.000965782880648 26.4905836169137 23.722445362253797 52-53 24.06922532932412 25.938228321692307 26.386992651790877 23.6055536971927 54-55 23.902435809923386 25.85520114814549 26.411481818454497 23.83088122347663 56-57 23.712046981573856 26.23289364610753 26.368712824129958 23.686346548188652 58-59 23.84103763187973 25.8988272560154 26.511080965008553 23.749054147096317 60-61 23.792505200776677 25.91015012094433 26.634498163395847 23.66284651488314 62-63 23.752512018149247 25.987810519982073 26.639094682747217 23.62058277912146 64-65 23.637253144258576 26.061676527376054 26.55358720086966 23.747483127495713 66-67 23.67073225045014 26.259781078356788 26.322837164871103 23.74664950632197 68-69 23.65723483949897 25.920755200593344 26.723169464766304 23.698840495141386 70-71 23.634117782594537 25.767530131200044 26.53463420708379 24.06371787912163 72-73 23.70059096221155 26.093775457082806 26.325552077138898 23.880081503566746 74-75 23.54416561914033 26.284344548454797 26.188067561585914 23.98342227081896 76-77 23.77006802999849 26.198413202504838 26.163832481791623 23.86768628570505 78-79 23.692474817077372 26.295458851535447 26.24937706569111 23.762689265696068 80-81 23.675882493156355 26.36257643214335 26.129505119411274 23.832035955289026 82-83 23.837882006269705 26.382439368448416 26.13205557842996 23.647623046851923 84-85 23.80332257016322 26.339560217323626 25.860330257384433 23.996786955128716 86-87 23.678348778011372 26.106127949866014 26.306806767979023 23.908716504143595 88-89 23.80017667905096 26.37111971777064 25.98514570307634 23.843557900102052 90-91 23.674449592945745 26.471826448121412 26.114541377030225 23.739182581902615 92-93 23.612238902776898 26.278061121585676 26.149525157610388 23.96017481802703 94-95 23.733714117431052 26.101904587042558 26.30746073845695 23.85692055706944 96-97 23.787404072602598 26.00174425844475 26.185304963456012 24.02554670549664 98-99 23.427593923523215 26.042582239039717 26.117002082392197 24.41282175504487 100-101 23.41709289492323 26.082238023855147 26.154929171009616 24.345739910212004 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 79.0 1 55.5 2 81.5 3 142.0 4 121.5 5 109.0 6 245.5 7 482.5 8 779.5 9 901.5 10 783.0 11 746.0 12 816.5 13 911.5 14 1022.0 15 1158.5 16 1212.0 17 1270.5 18 1498.0 19 1687.0 20 1926.5 21 2364.0 22 2859.0 23 3481.5 24 4351.5 25 5513.5 26 7483.0 27 10102.0 28 12140.5 29 14556.0 30 17694.0 31 21241.5 32 25792.5 33 29300.5 34 32389.0 35 36157.5 36 41189.0 37 46011.5 38 49183.0 39 51703.5 40 54618.5 41 57959.5 42 60916.0 43 64376.0 44 68113.0 45 72502.5 46 76156.0 47 79356.0 48 83017.5 49 84063.0 50 82364.0 51 81645.5 52 83654.5 53 86228.0 54 89082.5 55 86915.0 56 79570.0 57 73220.5 58 69389.5 59 64590.5 60 56800.0 61 48739.5 62 40563.0 63 31705.5 64 23736.0 65 17952.5 66 13047.0 67 10436.5 68 7920.0 69 5720.5 70 4620.5 71 3053.5 72 2301.0 73 2042.0 74 1553.5 75 1130.5 76 776.5 77 438.5 78 270.5 79 169.5 80 98.5 81 53.0 82 25.5 83 11.0 84 6.0 85 3.5 86 2.0 87 2.0 88 1.5 89 0.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25363316672892744 2 0.019405368606567285 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.999041093026701E-4 12-13 2.272291405921228E-5 14-15 0.0 16-17 4.0901245306582107E-4 18-19 0.0 20-21 4.544582811842456E-5 22-23 4.544582811842456E-5 24-25 0.0025449663746317753 26-27 0.0025222434605725627 28-29 0.002385905976217289 30-31 0.0012724831873158877 32-33 0.001704218554440921 34-35 0.001886001866914619 36-37 9.089165623684912E-5 38-39 6.816874217763684E-5 40-41 6.589645077171561E-4 42-43 0.0037265579057108133 44-45 0.003999232874421361 46-47 0.01386097757611949 48-49 0.013906423404237913 50-51 0.006112463881928102 52-53 0.0071804408427110795 54-55 1.8178331247369823E-4 56-57 0.003181207968289719 58-59 0.001227037359197463 60-61 0.0013406519294935245 62-63 0.0 64-65 0.0010225311326645527 66-67 0.0 68-69 0.0 70-71 1.5906039841448596E-4 72-73 0.0012497602732566755 74-75 0.0 76-77 0.18685052230890256 78-79 0.09800392833738256 80-81 0.0010225311326645527 82-83 0.0 84-85 7.725790780132175E-4 86-87 0.11959069669363423 88-89 0.5835017101265121 90-91 0.30882712497875403 92-93 0.29464802660580564 94-95 0.002272291405921228 96-97 0.0027267496871054737 98-99 0.00309031631205287 100-101 7.498561639540052E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2200422.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.7961905793666 #Duplication Level Percentage of deduplicated Percentage of total 1 69.29600808025269 31.7349319243258 2 15.219515418540189 13.939916572661499 3 6.057092994925264 8.321753554576313 4 2.976764872019339 5.45297965555846 5 1.7029388021288272 3.8994054963645017 6 1.0541648891016098 2.896604169802451 7 0.7062707759404827 2.264115773892523 8 0.49813554238687013 1.8250168186804205 9 0.3674166700802693 1.5143655460529044 >10 1.9005523322135387 15.701336798090379 >50 0.14306815085767408 4.520587734423538 >100 0.07400113483335455 6.056970444020406 >500 0.0030775716662723857 1.0433730169903606 >1k 9.927650536362474E-4 0.828642494560383 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4005 0.18201054161429037 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3001 0.1363829301833921 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3633748435527368E-4 2 0.0 0.0 0.0 0.0 1.3633748435527368E-4 3 0.0 0.0 0.0 0.0 1.3633748435527368E-4 4 0.0 0.0 0.0 0.0 1.8178331247369823E-4 5 0.0 0.0 0.0 0.0 1.8178331247369823E-4 6 0.0 0.0 0.0 0.0 3.181207968289719E-4 7 0.0 0.0 0.0 0.0 3.181207968289719E-4 8 0.0 0.0 0.0 0.0 3.181207968289719E-4 9 0.0 0.0 0.0 0.0 3.181207968289719E-4 10-11 0.0 0.0 0.0 0.0 3.408437108881842E-4 12-13 0.0 0.0 0.0 0.0 9.543623904869156E-4 14-15 0.0 0.0 0.0 4.544582811842456E-5 0.0011134227889014016 16-17 0.0 0.0 0.0 4.544582811842456E-5 0.001227037359197463 18-19 0.0 0.0 0.0 6.816874217763684E-5 0.0012952061013750999 20-21 0.0 0.0 0.0 9.089165623684912E-5 0.0014769894138487983 22-23 0.0 0.0 0.0 1.3633748435527368E-4 0.0016133268982040719 24-25 0.0 0.0 0.0 2.7267496871054736E-4 0.0016814956403817087 26-27 0.0 0.0 0.0 3.862895390066087E-4 0.0016814956403817087 28-29 0.0 0.0 0.0 7.725790780132174E-4 0.0016814956403817087 30-31 0.0 0.0 0.0 0.002454074718394926 0.0017269414685001333 32-33 0.0 0.0 0.0 0.005521668116388583 0.0018178331247369823 34-35 0.0 0.0 0.0 0.009134611451803335 0.0019768935231514683 36-37 0.0 0.0 0.0 0.016019654411744653 0.0019996164372106805 38-39 0.0 0.0 0.0 0.027085713558581036 0.0020450622653291053 40-41 0.0 0.0 0.0 0.04042406411133864 0.0020905080934475297 42-43 0.0 0.0 0.0 0.05555752487477402 0.002113231007506742 44-45 0.0 0.0 0.0 0.0706909856382094 0.0021813997496843785 46-47 0.0 0.0 0.0 0.09052808961190173 0.0022950143199804403 48-49 0.0 0.0 0.0 0.11316011201487715 0.0024995205465133505 50-51 0.0 0.0 0.0 0.14108657339364905 0.0025904122027501997 52-53 0.0 0.0 0.0 0.16799050363975637 0.0029767017417568088 54-55 0.0 0.0 0.0 0.1956215671357585 0.002999424655816021 56-57 0.0 0.0 0.0 0.22900152788874134 0.0030675933979936575 58-59 0.0 0.0 0.0 0.2662898298599087 0.0030903163120528697 60-61 0.0 0.0 0.0 0.30705473768213554 0.0030903163120528697 62-63 0.0 0.0 0.0 0.35145531175383626 0.0033175454526449925 64-65 0.0 0.0 0.0 0.3981281772314583 0.0034084371088818417 66-67 0.0 0.0 0.0 0.45382203959058764 0.0034766058510594788 68-69 0.0 0.0 0.0 0.5190140800264677 0.0035674975072963275 70-71 0.0 0.0 0.0 0.592590875750197 0.0036583891635331768 72-73 0.0 0.0 0.0 0.6678491671143081 0.003885618304125299 74-75 0.0 0.0 0.0 0.7417440836348663 0.004181016186895059 76-77 0.0 0.0 0.0 0.8287728444816493 0.004362799499368757 78-79 0.0 0.0 0.0 0.932775622130664 0.004499136983724031 80-81 0.0 0.0 0.0 1.0484352546920546 0.004544582811842456 82-83 0.0 0.0 0.0 1.1673669868779717 0.004771811952434578 84-85 0.0 0.0 0.0 1.2899343853133627 0.004771811952434578 86-87 0.0 0.0 0.0 1.419182320482162 0.004976318178967489 88-89 0.0 0.0 0.0 1.5651315974844824 0.005180824405500399 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3245 0.0 44.415493 1 GTATCAA 5305 0.0 36.94183 1 TCAACGC 6555 0.0 29.238104 4 ATCAACG 6560 0.0 29.215816 3 TACATGG 8555 0.0 28.954718 2 CAACGCA 6740 0.0 28.365189 5 TATCAAC 6865 0.0 28.340801 2 GTACATG 9000 0.0 28.01173 1 AACGCAG 6965 0.0 27.51698 6 ACATGGG 8750 0.0 27.108313 3 TACCTGG 1920 0.0 26.19832 2 GTACCTG 2060 0.0 25.3999 1 CATGGGG 5075 0.0 24.023571 4 GTGTAGC 1315 0.0 23.15052 1 ACGCAGA 8475 0.0 22.502296 7 CGCAGAG 8655 0.0 21.924688 8 AGCGTAC 145 6.090319E-4 19.682932 1 AGTACAT 5840 0.0 19.663977 2 GAGTACA 6800 0.0 19.516497 1 GTAGCCC 1345 0.0 19.39907 3 >>END_MODULE