FastQCFastQC Report
Thu 26 May 2016
SRR936298_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936298_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2309276
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34270.14840149033723124No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24170.1046648386767108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60150.036.3597871
GGTATCA37150.034.532291
ATCAACG69550.030.7569583
TCAACGC70550.030.2539144
CAACGCA71750.029.6819695
TATCAAC73750.029.198522
AACGCAG74350.028.961016
GTACATG93000.025.5526351
TACATGG90600.025.5441912
ACATGGG90850.024.744053
ACGCAGA87150.024.3278267
CGCAGAG88750.023.8897558
GTACCTG23500.023.3671151
GTAGGAC5500.021.511933
TACCTGG23600.021.0566272
AGTACAT64250.020.4777932
GAGTACA71150.020.2927041
CATGGGG52300.019.9982814
GCAGAGT104600.019.7720579
GTGTAGC14050.019.5418931
Produced by FastQC (version 0.11.5)