##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936298_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2309276 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.67525492838448 33.0 31.0 34.0 30.0 34.0 2 31.773278724587275 34.0 31.0 34.0 30.0 34.0 3 31.84168457992895 34.0 31.0 34.0 30.0 34.0 4 35.32458181698507 37.0 35.0 37.0 33.0 37.0 5 35.30508219892295 37.0 35.0 37.0 33.0 37.0 6 35.39168856386157 37.0 35.0 37.0 33.0 37.0 7 35.33655873096156 37.0 35.0 37.0 33.0 37.0 8 35.33359979491408 37.0 35.0 37.0 33.0 37.0 9 37.00151605957885 39.0 37.0 39.0 33.0 39.0 10-11 36.91817846805665 39.0 37.0 39.0 33.0 39.0 12-13 36.82441661369191 39.0 37.0 39.0 33.0 39.0 14-15 38.075021781718604 40.0 38.0 41.0 33.0 41.0 16-17 38.02251874613515 40.0 38.0 41.0 33.0 41.0 18-19 37.96386183375222 40.0 38.0 41.0 33.0 41.0 20-21 37.87641884296204 40.0 38.0 41.0 33.0 41.0 22-23 37.71965196018146 40.0 38.0 41.0 32.0 41.0 24-25 37.86825741054772 40.0 38.0 41.0 33.0 41.0 26-27 37.8230573132012 40.0 38.0 41.0 33.0 41.0 28-29 37.69581353636378 40.0 38.0 41.0 32.0 41.0 30-31 37.55573586699901 40.0 38.0 41.0 32.0 41.0 32-33 37.38799693063973 40.0 37.0 41.0 32.0 41.0 34-35 37.13267080245063 40.0 37.0 41.0 31.0 41.0 36-37 36.937367599195596 40.0 37.0 41.0 30.5 41.0 38-39 36.779209371248825 40.0 37.0 41.0 30.0 41.0 40-41 36.60102062291385 40.0 36.0 41.0 30.0 41.0 42-43 36.42100987495648 40.0 36.0 41.0 30.0 41.0 44-45 36.24345833932367 40.0 36.0 41.0 29.5 41.0 46-47 36.0017284638129 39.5 35.0 41.0 28.5 41.0 48-49 35.84380472494409 39.0 35.0 41.0 28.0 41.0 50-51 35.54172065184066 39.0 35.0 40.5 28.0 41.0 52-53 35.563736859517874 39.0 35.0 40.5 28.0 41.0 54-55 35.64014955336651 39.0 35.0 41.0 28.0 41.0 56-57 35.475166459098006 39.0 35.0 41.0 28.0 41.0 58-59 35.23654361799976 39.0 35.0 41.0 27.0 41.0 60-61 34.937970169005354 38.0 35.0 40.5 26.5 41.0 62-63 34.62919720293287 37.5 34.0 40.0 26.0 41.0 64-65 34.29295177362948 37.0 34.0 40.0 26.0 41.0 66-67 33.93465830849149 36.5 34.0 39.5 26.0 41.0 68-69 33.5531519402618 36.0 34.0 39.0 25.0 41.0 70-71 33.14727819455102 35.5 33.0 39.0 24.0 40.5 72-73 32.74418172622069 35.0 33.0 38.0 23.0 40.0 74-75 32.32597835858512 35.0 33.0 37.0 22.0 39.5 76-77 31.936278946301783 35.0 32.0 37.0 20.5 39.0 78-79 31.54862389770647 35.0 32.0 36.0 20.0 39.0 80-81 31.162686053984018 35.0 32.0 36.0 18.0 37.5 82-83 30.8199693323795 35.0 31.0 35.0 17.0 37.0 84-85 30.51162485558244 35.0 31.0 35.0 12.5 36.5 86-87 30.200545105911985 34.0 31.0 35.0 7.0 36.0 88-89 29.877957636939023 34.0 31.0 35.0 4.5 36.0 90-91 29.530353669288555 34.0 30.0 35.0 2.0 35.5 92-93 29.23150372670915 34.0 30.0 35.0 2.0 35.0 94-95 28.861520450565457 34.0 29.5 35.0 2.0 35.0 96-97 28.44740407816129 34.0 29.0 35.0 2.0 35.0 98-99 27.964768611460908 34.0 29.0 35.0 2.0 35.0 100-101 24.7925334607037 30.5 23.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3333852078852537 1101 2 0.2683676802679642 1101 3 0.29956032269764776 1101 4 0.2108598609686041 1101 5 0.35147241519738515 1101 6 0.3298089395953383 1101 7 0.4139579464354597 1101 8 0.3670374176053599 1101 9 0.4895313779745223 1101 10-11 0.6751578740396198 1101 12-13 0.5564498667464335 1101 14-15 0.7119014841511202 1101 16-17 0.6934756572608833 1101 18-19 0.7334240337664895 1101 20-21 0.7349988571481987 1101 22-23 0.718964471426979 1101 24-25 0.6415127238154668 1101 26-27 0.60403191339784 1101 28-29 0.6290485349674242 1101 30-31 0.6936398899829328 1101 32-33 0.5690717157623837 1101 34-35 0.5260373861572774 1101 36-37 0.3049594716009878 1101 38-39 0.4091136302041889 1101 40-41 0.40657379283751993 1101 42-43 0.5031536408945811 1101 44-45 0.48393824589556544 1101 46-47 0.30606066035643664 1101 48-49 0.40418432165089513 1101 50-51 0.4511628983120701 1101 52-53 0.5715580866276468 1101 54-55 0.4846937752295233 1101 56-57 0.43379280623050676 1101 58-59 0.4766132622137107 1101 60-61 0.3432699742384102 1101 62-63 0.28387830740322073 1101 64-65 0.27884472646817215 1101 66-67 0.32371970930297067 1101 68-69 0.34197051469517703 1101 70-71 0.3849541402318266 1101 72-73 0.4742941374932741 1101 74-75 0.3778561130023874 1101 76-77 0.3770919807034758 1101 78-79 0.4401548206157173 1101 80-81 0.35903091421015887 1101 82-83 0.6053921925829364 1101 84-85 0.503298347184117 1101 86-87 0.43127075793854175 1101 88-89 0.36675127638416427 1101 90-91 0.5971625883275742 1101 92-93 0.4898199947374664 1101 94-95 0.7817918193479727 1101 96-97 0.8064279613335827 1101 98-99 0.929635119006246 1101 100-101 1.0323113619474498 1102 1 0.33676063650959165 1102 2 0.2882835509043318 1102 3 0.23899161921884016 1102 4 0.2509755024951801 1102 5 0.2399087982010215 1102 6 0.28462900728757035 1102 7 0.188648277068296 1102 8 0.25636605854959527 1102 9 0.3013277013514539 1102 10-11 0.31870082677060907 1102 12-13 0.4742025857992047 1102 14-15 0.4783411071377017 1102 16-17 0.45533958869017255 1102 18-19 0.5064335371553099 1102 20-21 0.38673015129717214 1102 22-23 0.3324013709607314 1102 24-25 0.438538581695461 1102 26-27 0.5223084322450688 1102 28-29 0.39434107849177735 1102 30-31 0.43793376271143813 1102 32-33 0.17911443854182352 1102 34-35 0.24543450751583862 1102 36-37 0.17338739116309654 1102 38-39 0.2847576667301368 1102 40-41 0.33116804886962825 1102 42-43 0.31558510500109804 1102 44-45 0.2611186342803009 1102 46-47 0.46300125633270284 1102 48-49 0.4513626515674787 1102 50-51 0.268125670647791 1102 52-53 0.18208290417773298 1102 54-55 0.30076658531402956 1102 56-57 0.2691746597242002 1102 58-59 0.32090583405157247 1102 60-61 0.1838419988145361 1102 62-63 0.4291242790422771 1102 64-65 0.14222059892579608 1102 66-67 0.24854047335431062 1102 68-69 0.3954700253307166 1102 70-71 0.3956528179912624 1102 72-73 0.30794083242753345 1102 74-75 0.25011821645073695 1102 76-77 0.31209450978794706 1102 78-79 0.3264600386411658 1102 80-81 0.3453436314875944 1102 82-83 0.38671668292076333 1102 84-85 0.3927842428511532 1102 86-87 0.4242830620684117 1102 88-89 0.3142771736734886 1102 90-91 0.3198505804181586 1102 92-93 0.44999514053287726 1102 94-95 0.5374325194720129 1102 96-97 0.6562703622573665 1102 98-99 0.798411218124933 1102 100-101 1.0046360611930112 1103 1 0.43418403179282095 1103 2 0.3818384441258189 1103 3 0.48583377558815144 1103 4 0.40938642453387786 1103 5 0.36659884339901794 1103 6 0.40968191288914824 1103 7 0.42616595241901933 1103 8 0.4348535599795582 1103 9 0.43601961841326187 1103 10-11 0.4629517019830658 1103 12-13 0.563703915450084 1103 14-15 0.6609775442910362 1103 16-17 0.6491897896758658 1103 18-19 0.5870237474756124 1103 20-21 0.6556686271167536 1103 22-23 0.6958960240667622 1103 24-25 0.7048105904874831 1103 26-27 0.7710504957871294 1103 28-29 0.7398444915053304 1103 30-31 0.7935765203700598 1103 32-33 0.6707165004581199 1103 34-35 0.49477227639550847 1103 36-37 0.48729853149531266 1103 38-39 0.6886119123845873 1103 40-41 0.6323704792288112 1103 42-43 0.5178769768970284 1103 44-45 0.6878838159333682 1103 46-47 0.7833303742662991 1103 48-49 0.7756930760501533 1103 50-51 0.7743197954760959 1103 52-53 0.7833762839914016 1103 54-55 0.8601986317904036 1103 56-57 0.8782629834795515 1103 58-59 0.8635035837135092 1103 60-61 0.7894300904399927 1103 62-63 0.7739531687992312 1103 64-65 0.7477944835595878 1103 66-67 0.9380307339055989 1103 68-69 0.7954026471284976 1103 70-71 0.8872235009868348 1103 72-73 0.9056814760116652 1103 74-75 0.9093380722411197 1103 76-77 0.7479919456853921 1103 78-79 0.8574149733223564 1103 80-81 0.9423985097608529 1103 82-83 0.9599123393863138 1103 84-85 0.9046076719346701 1103 86-87 0.9119319976682441 1103 88-89 0.9125953623537058 1103 90-91 1.0192415081544794 1103 92-93 0.9447234818505379 1103 94-95 0.9767022010352662 1103 96-97 1.1632954107896865 1103 98-99 1.111009632426999 1103 100-101 1.0905076897812336 1104 1 0.2900309060091395 1104 2 0.19017948753534597 1104 3 0.26283469132497217 1104 4 0.23071946927710485 1104 5 0.25922011695757163 1104 6 0.24309666873173796 1104 7 0.36396641746271285 1104 8 0.2904754665260896 1104 9 0.3855257433356769 1104 10-11 0.3256655367132879 1104 12-13 0.39438566931698915 1104 14-15 0.5369521575183214 1104 16-17 0.4068966492242865 1104 18-19 0.23638534843080805 1104 20-21 0.3854942961173151 1104 22-23 0.44427252711720655 1104 24-25 0.45319384256801953 1104 26-27 0.4583653266742047 1104 28-29 0.32664080743477086 1104 30-31 0.3259902174437528 1104 32-33 0.3857105246063881 1104 34-35 0.3510682571231243 1104 36-37 0.34858086142153155 1104 38-39 0.37745545778980727 1104 40-41 0.42729859495408107 1104 42-43 0.38222135361699827 1104 44-45 0.25562193651556697 1104 46-47 0.33841240576698794 1104 48-49 0.37285081082936955 1104 50-51 0.3812757011024033 1104 52-53 0.41622585000509105 1104 54-55 0.4927539637728344 1104 56-57 0.3593384662897776 1104 58-59 0.4236375602491407 1104 60-61 0.2930523067314894 1104 62-63 0.374187158856607 1104 64-65 0.29300943306382266 1104 66-67 0.37234230560314785 1104 68-69 0.18941990428003663 1104 70-71 0.2215760168002845 1104 72-73 0.3600818317921153 1104 74-75 0.24437851550794676 1104 76-77 0.4193121672714355 1104 78-79 0.37176258247252747 1104 80-81 0.4446557643169804 1104 82-83 0.4944460607375696 1104 84-85 0.31844647525822367 1104 86-87 0.4685828826555358 1104 88-89 0.5789177495495892 1104 90-91 0.6879718782452429 1104 92-93 0.6448476166426431 1104 94-95 0.5229765114560792 1104 96-97 0.7667025106272582 1104 98-99 0.721405412128238 1104 100-101 0.5621992974688759 1105 1 0.1603464820503504 1105 2 0.21115594328403375 1105 3 0.18093428684342783 1105 4 0.0723792420287026 1105 5 0.14277686399123013 1105 6 0.12739654860541094 1105 7 0.19349289968912586 1105 8 0.1347252248056634 1105 9 0.15622199293030548 1105 10-11 0.2881506191594241 1105 12-13 0.23108841086462917 1105 14-15 0.1930806688787854 1105 16-17 0.21750595112090565 1105 18-19 0.22968374643055967 1105 20-21 0.16650529887511567 1105 22-23 0.22014192897501772 1105 24-25 0.22211685932213499 1105 26-27 0.2768699815982103 1105 28-29 0.29089796699382475 1105 30-31 0.3838647789318088 1105 32-33 0.47920068092778934 1105 34-35 0.2612001510670865 1105 36-37 0.30962648515500035 1105 38-39 0.3831166738140226 1105 40-41 0.22505192986382383 1105 42-43 0.21739576100033275 1105 44-45 0.3760116524816439 1105 46-47 0.36514762094843434 1105 48-49 0.25403508026737853 1105 50-51 0.14016642451621664 1105 52-53 0.19697346244524283 1105 54-55 0.3958249400089926 1105 56-57 0.3839038029220134 1105 58-59 0.29268749900713686 1105 60-61 0.19273538311190208 1105 62-63 0.0810722210544057 1105 64-65 0.23576139150200248 1105 66-67 0.19242938274265242 1105 68-69 0.2538313765868452 1105 70-71 0.18222534154332237 1105 72-73 0.22192996964915324 1105 74-75 0.32870224959906835 1105 76-77 0.2680964227482576 1105 78-79 0.341791214467829 1105 80-81 0.3381338548423507 1105 82-83 0.3435379708987014 1105 84-85 0.29844903635056497 1105 86-87 0.37183727286014445 1105 88-89 0.31793885752713535 1105 90-91 0.46144545681010385 1105 92-93 0.38007296849812633 1105 94-95 0.26924981005748805 1105 96-97 0.3155203284332764 1105 98-99 0.3415801627626749 1105 100-101 0.20126291310716482 1106 1 0.09166690378889086 1106 2 0.009963049274993807 1106 3 0.14508557928945365 1106 4 0.1255419705347407 1106 5 0.12270874805305709 1106 6 0.011632282810403183 1106 7 0.12227239374838916 1106 8 0.07153853105943142 1106 9 0.08753598194054035 1106 10-11 0.15011735707410878 1106 12-13 0.0859220294561851 1106 14-15 0.049726875033080375 1106 16-17 0.1619694349623373 1106 18-19 0.05698883698119772 1106 20-21 0.13953542001448227 1106 22-23 0.08230874692170431 1106 24-25 0.16291867894134526 1106 26-27 0.09465330335263644 1106 28-29 0.15787857298344932 1106 30-31 0.15416247672489902 1106 32-33 0.24207223753268892 1106 34-35 0.043047590708134464 1106 36-37 0.08352850716114091 1106 38-39 0.10940585911119882 1106 40-41 0.09497850359753102 1106 42-43 -0.024425579509262718 1106 44-45 0.13295023722817945 1106 46-47 0.0928311555596224 1106 48-49 0.24414864232191036 1106 50-51 0.13770856775932572 1106 52-53 0.06244071364727688 1106 54-55 0.23834687117614095 1106 56-57 0.3249598262182545 1106 58-59 0.16388586748978895 1106 60-61 0.25629750419093256 1106 62-63 0.16267193411035663 1106 64-65 0.1041624104461718 1106 66-67 0.158104677250698 1106 68-69 0.17437819841978808 1106 70-71 0.3052208812226098 1106 72-73 0.2792361515454118 1106 74-75 0.1873056687938046 1106 76-77 0.22760162172363607 1106 78-79 0.21212770826711846 1106 80-81 0.257152681885362 1106 82-83 0.26820118429936457 1106 84-85 0.30553590657536844 1106 86-87 0.30396466927325605 1106 88-89 0.25898611900378654 1106 90-91 0.2722291938082293 1106 92-93 0.1856215737495397 1106 94-95 0.1550877372460988 1106 96-97 0.2846290362179431 1106 98-99 0.3461593780408023 1106 100-101 0.5168087545035931 1107 1 0.05133825546956672 1107 2 0.11910891965443682 1107 3 0.20049600890505914 1107 4 0.10953438055512521 1107 5 0.15526574171644825 1107 6 0.21683240979942298 1107 7 0.11874624630532793 1107 8 0.23228974819046755 1107 9 0.2575535438614551 1107 10-11 0.265460823837671 1107 12-13 0.125207193211466 1107 14-15 0.3053106299809727 1107 16-17 0.10844663657942277 1107 18-19 0.37330229249558045 1107 20-21 0.45068811146602883 1107 22-23 0.32088845180069114 1107 24-25 0.3419415424703516 1107 26-27 0.34046491367251264 1107 28-29 0.35694831417909256 1107 30-31 0.35338797215641193 1107 32-33 0.3738988149400271 1107 34-35 0.3659155698393519 1107 36-37 0.3770139887559836 1107 38-39 0.3196061787858753 1107 40-41 0.5042632452794749 1107 42-43 0.30923027290454286 1107 44-45 0.49561743790936674 1107 46-47 0.5456002318980069 1107 48-49 0.516157472830109 1107 50-51 0.5807518357813919 1107 52-53 0.5265681504326238 1107 54-55 0.5281126021245726 1107 56-57 0.556179856717641 1107 58-59 0.5546257620448571 1107 60-61 0.5986643538172203 1107 62-63 0.6880181259995766 1107 64-65 0.3978148862732951 1107 66-67 0.47821862787545655 1107 68-69 0.5485340517511403 1107 70-71 0.4559808884896057 1107 72-73 0.4220737964496095 1107 74-75 0.420125219597665 1107 76-77 0.5000272496494702 1107 78-79 0.539350841923067 1107 80-81 0.6699537073400528 1107 82-83 0.7038088506191968 1107 84-85 0.6356568948142076 1107 86-87 0.4625890038195877 1107 88-89 0.6724282830288288 1107 90-91 0.8718545578464756 1107 92-93 0.5710291222558581 1107 94-95 0.6043765436777626 1107 96-97 0.8512010116496036 1107 98-99 0.9805117432113128 1107 100-101 1.1477411787276637 1108 1 -0.18949261385282767 1108 2 -0.06665904982917681 1108 3 0.024755015013344206 1108 4 -0.10836767506494738 1108 5 -0.04799439851795739 1108 6 -0.12318637951984357 1108 7 -0.06969423580153489 1108 8 -0.11351919220353324 1108 9 -0.10775590647186561 1108 10-11 -0.08073469054191662 1108 12-13 -0.08751593544747038 1108 14-15 -0.11959284186865915 1108 16-17 -0.07983455815060125 1108 18-19 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-1.5597539294309435 2316 100-101 -1.4384526723812137 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21633.0 3 4675.0 4 3711.0 5 3124.0 6 3018.0 7 3197.0 8 3801.0 9 4533.0 10 5453.0 11 6283.0 12 7879.0 13 11264.0 14 11734.0 15 9781.0 16 11073.0 17 13210.0 18 12422.0 19 10232.0 20 10133.0 21 10586.0 22 11256.0 23 12697.0 24 14320.0 25 16727.0 26 19599.0 27 23546.0 28 28780.0 29 34839.0 30 42786.0 31 52721.0 32 67114.0 33 88065.0 34 122809.0 35 184557.0 36 308505.0 37 512861.0 38 504049.0 39 96224.0 40 79.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.37776899762416 17.5403208337449 12.578219904723781 24.503690263907156 2 15.813854381226754 18.886325957519052 40.054863862436704 25.244955798817493 3 20.39096818283986 22.8261392086831 27.91821872061816 28.864673887858878 4 12.773875148486852 14.795548274304531 35.6846548500797 36.74592172712891 5 14.388779898400655 36.97449622575322 33.59031991234566 15.046403963500458 6 34.81794087842227 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23.51832267552897 28-29 23.183649374029144 26.217990877290397 27.127013472270978 23.471346276409484 30-31 23.463362323786736 26.006628204256447 27.02909714104554 23.500912330911277 32-33 23.196921066046016 25.922214171532808 27.103384566611854 23.777480195809318 34-35 23.60681304420221 26.04950200796761 27.034219137334826 23.309465810495354 36-37 23.714247429713005 25.791859829738062 26.95407939524297 23.539813345305962 38-39 23.420660354724287 26.035685596620812 26.971601044696285 23.57205300395861 40-41 23.665004687438472 25.951569590240208 27.067982761017657 23.315442961303667 42-43 23.58018498101721 25.88742245685908 26.88912016108611 23.643272401037592 44-45 23.473814139057264 26.100896661458574 26.950147833497855 23.475141365986307 46-47 23.735514646860597 26.221652422024373 26.60148053761693 23.4413523934981 48-49 23.742049987454937 26.183016921365187 26.501637604116958 23.573295487062918 50-51 23.671255665518 26.350638163773677 26.509901625758285 23.468204544950034 52-53 23.80423597161628 26.22652270691168 26.462712800114723 23.506528521357318 54-55 23.73489797374802 26.228092162176747 26.514858775058354 23.522151089016884 56-57 23.58345188710997 26.522860337305527 26.344893695390294 23.548794080194206 58-59 23.644366258467407 26.180237435515423 26.542865273358906 23.63253103265826 60-61 23.519758740689753 26.225376086576276 26.686156654950626 23.568708517783346 62-63 23.444202168820063 26.31902978945951 26.743008506332703 23.493759535387724 64-65 23.51173626381278 26.349227555012856 26.593248020094318 23.545788161080054 66-67 23.485391143303815 26.48797294513568 26.418930574132776 23.60770533742773 68-69 23.556451333901524 26.257153419400293 26.62141453245396 23.564980714244218 70-71 23.555083053356203 26.145165206086663 26.48923034351693 23.810521397040205 72-73 23.560160899869658 26.31100998847141 26.468263525425012 23.66056558623392 74-75 23.503123884152235 26.49947636072773 26.36654836096447 23.630851394155563 76-77 23.721122498153427 26.334280098034203 26.272165661703678 23.672431742108692 78-79 23.635201842161774 26.44604760440864 26.354452967894666 23.56429758553492 80-81 23.59177299187429 26.470277241836655 26.336320969410888 23.601628796878167 82-83 23.714763685004428 26.55214059736518 26.298366678509737 23.43472903912065 84-85 23.57850738189501 26.633173154221655 26.193755734215664 23.59456372966767 86-87 23.629050014838487 26.46089471288616 26.346494766496175 23.563560505779176 88-89 23.728096202083215 26.523552532883848 26.10085577325436 23.647495491778574 90-91 23.495803974796857 26.692775558142305 26.224178055297507 23.587242411763324 92-93 23.621215613226752 26.552052613125994 26.285300286252212 23.541431487395045 94-95 23.57776164877827 26.50706789583567 26.360383928206225 23.55478652717983 96-97 23.680736705899907 26.373476986749672 26.263222540193716 23.682563767156704 98-99 23.50180894063608 26.387936481009653 26.20235559194823 23.907898986406032 100-101 23.57980800706461 26.53337146946264 26.057467376752697 23.829353146720056 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 79.0 1 58.0 2 95.5 3 167.0 4 141.5 5 140.0 6 270.0 7 490.5 8 819.0 9 999.5 10 943.5 11 965.0 12 1094.5 13 1297.0 14 1565.5 15 1808.5 16 1978.0 17 2095.0 18 2196.5 19 2276.0 20 2381.0 21 2552.5 22 2849.5 23 3348.5 24 4270.5 25 5600.0 26 7586.5 27 10033.0 28 12378.5 29 15337.5 30 18867.0 31 22827.5 32 27709.5 33 31165.0 34 33751.5 35 37738.5 36 42665.0 37 47718.5 38 51879.0 39 55246.0 40 59498.0 41 64143.0 42 67860.0 43 73023.0 44 77708.0 45 81897.5 46 86918.0 47 90107.0 48 93271.0 49 94531.5 50 91385.0 51 88912.5 52 88531.5 53 88667.0 54 89490.0 55 85725.5 56 78483.0 57 71733.5 58 66258.5 59 60429.0 60 52803.0 61 45753.5 62 38094.0 63 28716.5 64 21176.0 65 16504.5 66 12103.5 67 9350.5 68 7416.0 69 5813.5 70 4536.0 71 3083.5 72 2364.0 73 2007.5 74 1600.0 75 1257.0 76 876.0 77 574.5 78 374.0 79 218.0 80 138.5 81 97.5 82 72.5 83 45.5 84 38.5 85 39.5 86 32.0 87 30.0 88 27.0 89 21.0 90 24.0 91 26.5 92 22.0 93 18.5 94 16.5 95 16.5 96 16.5 97 17.5 98 16.5 99 11.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.045035760125684415 2 0.012471441265574144 3 0.010349564105806322 4 0.006452238710314402 5 0.008920544794125951 6 0.005889291708743347 7 0.0061491134017761405 8 0.010003135181762596 9 0.015502694350956749 10-11 0.0023600470450478852 12-13 0.009570099026707938 14-15 0.009266973718169678 16-17 0.019638189631728732 18-19 0.012428137650068678 20-21 0.0038540217799864548 22-23 0.010133046028278993 24-25 0.021738414983743824 26-27 0.007361614635929182 28-29 0.01829577755105929 30-31 0.015372783504440353 32-33 0.012774566574112406 34-35 0.26313441961896283 36-37 0.4633486859084839 38-39 0.45977613762928293 40-41 0.48121142730448857 42-43 0.47105672946845684 44-45 0.48723062985974824 46-47 0.4934230468770298 48-49 0.5005681434354317 50-51 0.5120652533521328 52-53 0.5001784108958826 54-55 0.48673263828143537 56-57 0.4803453549943792 58-59 0.4785915585664078 60-61 0.4764263777911345 62-63 0.4732868656669883 64-65 0.4898938022133344 66-67 0.46280739071466553 68-69 0.4909114371777128 70-71 0.47088351500643494 72-73 0.4796524971462917 74-75 0.49433242280264467 76-77 0.48060517668741204 78-79 0.4809516056114557 80-81 0.48231566949987786 82-83 0.4835931261572891 84-85 0.4815145526130268 86-87 0.48491388643020583 88-89 0.49926903497026776 90-91 0.5240603548471469 92-93 0.5121302087753911 94-95 0.48231566949987786 96-97 0.4547962218461544 98-99 0.44509621197293003 100-101 0.4544064893066052 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2309276.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.533877610573285 #Duplication Level Percentage of deduplicated Percentage of total 1 71.55149368639108 36.87325918488185 2 14.644457100632433 15.093713197945654 3 5.621602601034533 8.691089412509822 4 2.7157669699153604 5.598160105858226 5 1.5660518362929414 4.0352361831667 6 0.9180144160480704 2.83853055368179 7 0.6150232263738007 2.2186172172925147 8 0.4411167708689872 1.8185966145546948 9 0.30755571854896374 1.42645848823208 >10 1.4908975528049375 13.422587959314331 >50 0.08622159036804339 3.07923778143696 >100 0.03935732024830904 3.7417710483309254 >500 0.0018519527723031646 0.6289818275293682 >1k 5.892577002779552E-4 0.5337604252650525 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3427 0.14840149033723124 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2417 0.1046648386767108 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.3303615505465784E-5 4.3303615505465784E-5 2 0.0 0.0 0.0 4.3303615505465784E-5 4.3303615505465784E-5 3 0.0 0.0 0.0 4.3303615505465784E-5 4.3303615505465784E-5 4 0.0 0.0 0.0 4.3303615505465784E-5 4.3303615505465784E-5 5 0.0 0.0 0.0 4.3303615505465784E-5 1.7321446202186314E-4 6 0.0 0.0 0.0 4.3303615505465784E-5 2.598216930327947E-4 7 0.0 0.0 0.0 4.3303615505465784E-5 2.598216930327947E-4 8 0.0 0.0 0.0 4.3303615505465784E-5 2.598216930327947E-4 9 0.0 0.0 0.0 4.3303615505465784E-5 2.598216930327947E-4 10-11 0.0 0.0 0.0 8.660723101093157E-5 3.031253085382605E-4 12-13 0.0 0.0 0.0 1.0825903876366446E-4 9.093759256147815E-4 14-15 0.0 0.0 0.0 1.5156265426913024E-4 0.0010392867721311788 16-17 0.0 0.0 0.0 2.598216930327947E-4 0.0011691976186475762 18-19 0.0 0.0 0.0 3.6808073179645916E-4 0.0012991084651639735 20-21 0.0 0.0 0.0 6.495542325819868E-4 0.001602233773702234 22-23 0.0 0.0 0.0 0.001017634964378446 0.0016888410047131656 24-25 0.0 0.0 0.0 0.0014723229271858367 0.0016888410047131656 26-27 0.0 0.0 0.0 0.0016888410047131656 0.001818751851229563 28-29 0.0 0.0 0.0 0.0021002253520150905 0.0019053590822404945 30-31 0.0 0.0 0.0 0.0037241109334700574 0.0019053590822404945 32-33 0.0 0.0 0.0 0.006582149556830799 0.0019053590822404945 34-35 0.0 0.0 0.0 0.010046438797268062 0.001991966313251426 36-37 0.0 0.0 0.0 0.016152248583538736 0.002013618121004159 38-39 0.0 0.0 0.0 0.027649358500239904 0.0020785735442623576 40-41 0.0 0.0 0.0 0.041744685347269016 0.0020785735442623576 42-43 0.0 0.0 0.0 0.05726903150597849 0.002186832583026022 44-45 0.0 0.0 0.0 0.07387596805232463 0.0023600470450478852 46-47 0.0 0.0 0.0 0.09466170349494821 0.0025549133148224813 48-49 0.0 0.0 0.0 0.11754766428958686 0.0027281277768443444 50-51 0.0 0.0 0.0 0.14333496732309176 0.002814735007855276 52-53 0.0 0.0 0.0 0.16942539566513487 0.0031178603163935364 54-55 0.0 0.0 0.0 0.1970964059731275 0.003182815739651735 56-57 0.0 0.0 0.0 0.2301154127960452 0.003247771162909934 58-59 0.0 0.0 0.0 0.26787616551681137 0.0032694229706626667 60-61 0.0 0.0 0.0 0.3099239761726186 0.0032910747784153996 62-63 0.0 0.0 0.0 0.3509325000562947 0.003420985624931797 64-65 0.0 0.0 0.0 0.3980468337262415 0.003420985624931797 66-67 0.0 0.0 0.0 0.45228461214683735 0.003420985624931797 68-69 0.0 0.0 0.0 0.5170668209430141 0.003420985624931797 70-71 0.0 0.0 0.0 0.5923718083070192 0.0034859410481899956 72-73 0.0 0.0 0.0 0.6666808125143985 0.0038756735877391877 74-75 0.0 0.0 0.0 0.7456882590041207 0.0043087097427938455 76-77 0.0 0.0 0.0 0.8256483850349634 0.004373665166052044 78-79 0.0 0.0 0.0 0.9249652271967491 0.00441696878155751 80-81 0.0 0.0 0.0 1.03660194796984 0.004460272397062976 82-83 0.0 0.0 0.0 1.1587614473107588 0.004633486859084839 84-85 0.0 0.0 0.0 1.2810941611136997 0.004633486859084839 86-87 0.0 0.0 0.0 1.4107451859370643 0.004828353128859435 88-89 0.0 0.0 0.0 1.560813865471256 0.005023219398634031 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6015 0.0 36.359787 1 GGTATCA 3715 0.0 34.53229 1 ATCAACG 6955 0.0 30.756958 3 TCAACGC 7055 0.0 30.253914 4 CAACGCA 7175 0.0 29.681969 5 TATCAAC 7375 0.0 29.19852 2 AACGCAG 7435 0.0 28.96101 6 GTACATG 9300 0.0 25.552635 1 TACATGG 9060 0.0 25.544191 2 ACATGGG 9085 0.0 24.74405 3 ACGCAGA 8715 0.0 24.327826 7 CGCAGAG 8875 0.0 23.889755 8 GTACCTG 2350 0.0 23.367115 1 GTAGGAC 550 0.0 21.51193 3 TACCTGG 2360 0.0 21.056627 2 AGTACAT 6425 0.0 20.477793 2 GAGTACA 7115 0.0 20.292704 1 CATGGGG 5230 0.0 19.998281 4 GCAGAGT 10460 0.0 19.772057 9 GTGTAGC 1405 0.0 19.541893 1 >>END_MODULE