FastQCFastQC Report
Thu 26 May 2016
SRR936298_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936298_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2309276
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37570.16269168345403495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28320.1226358391114791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39000.035.4977261
GTATCAA59700.033.5490261
TCAACGC72550.027.3965784
CAACGCA73700.027.0334535
ATCAACG73600.027.0057323
TATCAAC76650.026.3694932
AACGCAG76850.026.0488366
TACATGG87750.025.250742
GTACATG92650.024.8526151
ATAACGC1151.293856E-424.7498823
ACATGGG86950.023.9505043
GTACCTG20800.021.7286361
ACGCAGA93100.021.3493217
CGCAGAG93650.021.1732858
GTACACG3201.8189894E-1120.8137451
GTGTAGC15400.020.3889751
CATGGGG50500.020.1021334
GAGTACA72500.019.7515261
CCTATAC5200.019.1573623
AGTACAT65300.018.8914172
Produced by FastQC (version 0.11.5)