##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936298_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2309276 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50457892430355 34.0 31.0 34.0 31.0 34.0 2 32.633272506188085 34.0 31.0 34.0 31.0 34.0 3 32.72181194452287 34.0 31.0 34.0 31.0 34.0 4 36.19510747091296 37.0 35.0 37.0 35.0 37.0 5 36.15841501838671 37.0 35.0 37.0 35.0 37.0 6 36.19239839672694 37.0 37.0 37.0 35.0 37.0 7 36.14242256014439 37.0 37.0 37.0 35.0 37.0 8 36.12192479374488 37.0 36.0 37.0 35.0 37.0 9 37.90080570707009 39.0 38.0 39.0 35.0 39.0 10-11 37.83053779626169 39.0 38.0 39.0 35.0 39.0 12-13 37.77814670052432 39.0 38.0 39.0 35.0 39.0 14-15 39.14622894794732 41.0 39.0 41.0 36.0 41.0 16-17 39.06916540075764 40.0 38.0 41.0 36.0 41.0 18-19 39.07781573099101 40.0 38.5 41.0 36.0 41.0 20-21 39.024592123245554 40.0 38.5 41.0 35.0 41.0 22-23 38.911425269218576 40.0 38.0 41.0 35.0 41.0 24-25 39.06937888758208 40.0 39.0 41.0 35.0 41.0 26-27 38.991307881777665 40.0 39.0 41.0 35.0 41.0 28-29 38.905084970354345 40.0 38.0 41.0 35.0 41.0 30-31 38.80048876790821 40.0 38.0 41.0 35.0 41.0 32-33 38.661370923181124 40.0 38.0 41.0 34.5 41.0 34-35 38.5339383425801 40.0 38.0 41.0 34.0 41.0 36-37 38.39590655252989 40.0 38.0 41.0 34.0 41.0 38-39 38.26585453622694 40.0 38.0 41.0 34.0 41.0 40-41 38.129337506647104 40.0 38.0 41.0 33.0 41.0 42-43 38.16421467161136 40.0 38.0 41.0 33.5 41.0 44-45 38.04921434250389 40.0 38.0 41.0 33.0 41.0 46-47 37.87647275596334 40.0 37.5 41.0 33.0 41.0 48-49 37.66582881387933 40.0 37.0 41.0 33.0 41.0 50-51 37.466437965838644 40.0 37.0 41.0 32.5 41.0 52-53 37.253247338126755 40.0 36.5 41.0 32.0 41.0 54-55 37.06101024736758 39.5 36.0 41.0 31.5 41.0 56-57 36.79788795276095 39.0 35.5 41.0 31.0 41.0 58-59 36.52981215757666 39.0 35.0 41.0 31.0 41.0 60-61 36.24064122261696 38.5 35.0 40.5 31.0 41.0 62-63 35.93668231948021 38.0 35.0 40.0 30.0 41.0 64-65 35.557055761199614 37.0 35.0 40.0 29.5 41.0 66-67 35.20302943433353 37.0 34.0 39.5 29.0 41.0 68-69 34.84678639538973 36.0 34.0 39.0 29.0 41.0 70-71 34.467318111823786 36.0 34.0 39.0 29.0 40.5 72-73 34.061036879091105 35.0 34.0 38.5 28.0 40.0 74-75 33.639449333903784 35.0 33.5 37.0 27.5 39.5 76-77 32.92272015125087 35.0 32.5 36.0 26.5 39.0 78-79 32.973809540306135 35.0 33.0 36.0 27.0 39.0 80-81 32.80879093707292 35.0 33.0 36.0 27.0 37.5 82-83 32.53180001004644 35.0 33.0 35.5 26.5 37.0 84-85 32.247484709493364 35.0 33.0 35.0 26.0 37.0 86-87 31.857030515191774 35.0 32.5 35.0 25.5 36.0 88-89 31.369987389987166 35.0 32.0 35.0 24.5 36.0 90-91 31.15238239171065 34.5 32.0 35.0 24.0 36.0 92-93 30.866246390643646 34.0 32.0 35.0 22.0 35.0 94-95 30.706106156215192 34.0 31.5 35.0 20.0 35.0 96-97 30.454105096142687 34.0 31.0 35.0 19.0 35.0 98-99 30.000963938481153 34.0 31.0 35.0 8.5 35.0 100-101 26.589526544250234 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15777173146329204 1101 2 0.14815735690416432 1101 3 0.21965418764700928 1101 4 0.24999628731782053 1101 5 0.13569029216800743 1101 6 0.13679788755663935 1101 7 0.16835252523378585 1101 8 0.18341638982925446 1101 9 0.22990154709524546 1101 10-11 0.23750673435156955 1101 12-13 0.3299739795329515 1101 14-15 0.32827262029789495 1101 16-17 0.4242450559665869 1101 18-19 0.3727224424820079 1101 20-21 0.3083805844165681 1101 22-23 0.3797923758965851 1101 24-25 0.30908316392167023 1101 26-27 0.30774751313413873 1101 28-29 0.4233522415825135 1101 30-31 0.3895984211018728 1101 32-33 0.45714421864489196 1101 34-35 0.45332095620324964 1101 36-37 0.3782562150092801 1101 38-39 0.36336560183944044 1101 40-41 0.34026477733447535 1101 42-43 0.31294177458446626 1101 44-45 0.359834016894105 1101 46-47 0.25014583711262617 1101 48-49 0.2814911385138785 1101 50-51 0.3953439776030123 1101 52-53 0.44204238979680355 1101 54-55 0.3204044563582613 1101 56-57 0.3709159669106228 1101 58-59 0.19011317011754159 1101 60-61 0.2831382974876533 1101 62-63 0.26055790350556407 1101 64-65 0.35471609174819463 1101 66-67 0.33578692559244416 1101 68-69 0.5081430439554353 1101 70-71 0.40366478661250227 1101 72-73 0.42184814593489506 1101 74-75 0.35177685692993776 1101 76-77 0.09983273976746432 1101 78-79 0.2009556537994328 1101 80-81 0.39916218421961247 1101 82-83 0.4286523450961468 1101 84-85 0.4459015019019361 1101 86-87 0.44950332648956604 1101 88-89 0.4608976233323183 1101 90-91 0.7104900697603647 1101 92-93 0.9412314513815225 1101 94-95 0.8153414915509174 1101 96-97 0.684546946922751 1101 98-99 0.6891835554524448 1101 100-101 0.7155842525098386 1102 1 0.0674447717289226 1102 2 0.14471994466646265 1102 3 0.1567224545622281 1102 4 0.09227324387649105 1102 5 0.10952096364949426 1102 6 0.09137690786354113 1102 7 0.13809993053085634 1102 8 0.08407961449231038 1102 9 0.09933191768838157 1102 10-11 0.13964646990346097 1102 12-13 0.20726058181318052 1102 14-15 0.19511651418501685 1102 16-17 0.22471176961836647 1102 18-19 0.2653553456796729 1102 20-21 0.22307353213530234 1102 22-23 0.2739213033428598 1102 24-25 0.16965753010096307 1102 26-27 0.2568107570809772 1102 28-29 0.23594249087142316 1102 30-31 0.21192507168341024 1102 32-33 0.19266587962207637 1102 34-35 0.2149943454136647 1102 36-37 0.17962704766346604 1102 38-39 0.2120597754563036 1102 40-41 0.022005705726577673 1102 42-43 0.14040388231811107 1102 44-45 0.19024646810114376 1102 46-47 0.2897131914913089 1102 48-49 0.29362615259793046 1102 50-51 0.19441605995397282 1102 52-53 -0.029159334725058272 1102 54-55 -0.06484474455052691 1102 56-57 0.09494655467597823 1102 58-59 0.03780833623744684 1102 60-61 -0.001977141253014736 1102 62-63 0.09572549265674724 1102 64-65 0.203077390404367 1102 66-67 0.19309567362293478 1102 68-69 0.13303619874535144 1102 70-71 0.16794033973972944 1102 72-73 0.1587984909387501 1102 74-75 0.2755262992687264 1102 76-77 0.0625564833940615 1102 78-79 0.1586724988794188 1102 80-81 0.2342379457954351 1102 82-83 0.2274344053549342 1102 84-85 0.206903019352616 1102 86-87 0.26787986087096627 1102 88-89 0.2698583877050531 1102 90-91 0.3189005573877459 1102 92-93 0.2777776020078271 1102 94-95 0.26114562180221057 1102 96-97 0.15870896290533665 1102 98-99 0.33441383988070683 1102 100-101 0.7910665194571926 1103 1 0.06373489381763164 1103 2 0.08623927247562335 1103 3 0.19427511617416826 1103 4 0.0361348722250483 1103 5 0.14490131446583376 1103 6 0.14900861964790835 1103 7 0.16578701005023078 1103 8 0.14658568334853328 1103 9 0.1751201648248113 1103 10-11 0.1870705199436955 1103 12-13 0.22391463514203025 1103 14-15 0.2973966004548032 1103 16-17 0.3297514713654337 1103 18-19 0.326897439266439 1103 20-21 0.35415156113386814 1103 22-23 0.3079068981945454 1103 24-25 0.306895834225962 1103 26-27 0.2786371436895436 1103 28-29 0.2735444445674986 1103 30-31 0.2851659434441842 1103 32-33 0.33575968801743983 1103 34-35 0.40256913287088736 1103 36-37 0.3814062324687555 1103 38-39 0.4662134674334055 1103 40-41 0.45312650394701137 1103 42-43 0.31121639623169983 1103 44-45 0.3521012884592878 1103 46-47 0.5600080026284999 1103 48-49 0.5679584672281877 1103 50-51 0.5526064400663628 1103 52-53 0.504335933605482 1103 54-55 0.38232291252877104 1103 56-57 0.4863961199790907 1103 58-59 0.49524863660536766 1103 60-61 0.4972024358628957 1103 62-63 0.43153528994644574 1103 64-65 0.47118098927008134 1103 66-67 0.42722640215993124 1103 68-69 0.47682180115763373 1103 70-71 0.44137007801234773 1103 72-73 0.41481255759004654 1103 74-75 0.45825156100475084 1103 76-77 0.4930383376066061 1103 78-79 0.46289916537807585 1103 80-81 0.4959395035982439 1103 82-83 0.6162261513645646 1103 84-85 0.6204258023021509 1103 86-87 0.360070265197006 1103 88-89 0.5920284846807427 1103 90-91 0.7215624040545272 1103 92-93 0.7255483767682058 1103 94-95 0.7636424697031465 1103 96-97 0.7635808986116572 1103 98-99 0.7021237691842437 1103 100-101 1.0829373035324146 1104 1 0.0194690710717893 1104 2 0.03996437003462461 1104 3 0.012870422780238755 1104 4 -0.009739587763739621 1104 5 0.03127369658218271 1104 6 0.049926464059794284 1104 7 0.006183815130427206 1104 8 0.05270880602292749 1104 9 -0.03839238801595002 1104 10-11 0.003075027822184495 1104 12-13 0.13186366646676362 1104 14-15 0.10437481340162691 1104 16-17 0.052724625144570325 1104 18-19 0.1443010092428736 1104 20-21 0.12269688939603896 1104 22-23 0.09135230884223233 1104 24-25 0.14763198527541022 1104 26-27 0.2104623490198989 1104 28-29 0.24127673967110042 1104 30-31 0.1004075741140511 1104 32-33 0.023249401666753045 1104 34-35 -0.024232899605443947 1104 36-37 0.09920749792373584 1104 38-39 0.058658839955000985 1104 40-41 0.11520339600208018 1104 42-43 0.05548060868264315 1104 44-45 0.06292133350491724 1104 46-47 0.030273907309940284 1104 48-49 -0.05467259360803922 1104 50-51 0.10514858657526105 1104 52-53 0.06632482778045556 1104 54-55 0.028933277286618875 1104 56-57 0.03881062693639592 1104 58-59 0.14646207669494515 1104 60-61 -0.08727576583553542 1104 62-63 -0.014161421902407767 1104 64-65 -0.1355656964233205 1104 66-67 -0.28166282825881694 1104 68-69 -0.03914166788172935 1104 70-71 -0.0936467318087395 1104 72-73 -0.18228644878696088 1104 74-75 -0.11639188299925252 1104 76-77 -0.006702712765076058 1104 78-79 0.08632945346220566 1104 80-81 0.091677218765021 1104 82-83 0.19146883819843907 1104 84-85 0.21004463365904513 1104 86-87 0.24186206874860616 1104 88-89 0.35541719873949873 1104 90-91 0.5414853751243953 1104 92-93 0.42255363082892927 1104 94-95 0.3932956003413999 1104 96-97 0.328354063710222 1104 98-99 0.11662291182061679 1104 100-101 0.48694584851493516 1105 1 0.042875097359043934 1105 2 0.10534942726278729 1105 3 0.05175418409105248 1105 4 0.05127265855694674 1105 5 -0.02642438530296687 1105 6 -0.009221362513578413 1105 7 0.08522148237288008 1105 8 0.03816929065649077 1105 9 0.10868111325384433 1105 10-11 0.08777876514471927 1105 12-13 0.11334428757326265 1105 14-15 0.14849136251923767 1105 16-17 0.14886694513825915 1105 18-19 0.10310498374382604 1105 20-21 0.11607364572636669 1105 22-23 -0.01614468799962765 1105 24-25 0.08256972276298313 1105 26-27 0.1747278298176056 1105 28-29 0.09071848857355747 1105 30-31 0.08606002510022392 1105 32-33 0.10982218164696178 1105 34-35 0.1444105235302331 1105 36-37 0.13255983141863936 1105 38-39 0.1171917038780137 1105 40-41 0.02412558987816027 1105 42-43 0.1791731629538731 1105 44-45 0.15727539619944508 1105 46-47 0.16132671273464183 1105 48-49 0.19407407760757422 1105 50-51 0.14505169961997666 1105 52-53 0.1410058643016825 1105 54-55 0.2201968319487193 1105 56-57 0.34188701197767557 1105 58-59 0.357311053379064 1105 60-61 0.19093629584610738 1105 62-63 0.1185728515922051 1105 64-65 0.22760401593226476 1105 66-67 0.10782025870805967 1105 68-69 0.07347034812556075 1105 70-71 0.04671118315697953 1105 72-73 0.030685137187958844 1105 74-75 0.00869112713485265 1105 76-77 0.05370289214389601 1105 78-79 -0.05447353219822304 1105 80-81 0.0967468178361841 1105 82-83 0.045444159716076626 1105 84-85 0.09738604182989974 1105 86-87 0.08794076593850164 1105 88-89 0.18242546612123434 1105 90-91 0.2978080718344316 1105 92-93 0.3135649363873547 1105 94-95 0.0791206476857802 1105 96-97 0.09224770071329047 1105 98-99 0.07648218118350414 1105 100-101 0.3982458363335688 1106 1 -0.06294461411756203 1106 2 -0.05763527881476449 1106 3 -0.04882595927449529 1106 4 -0.03826115940483277 1106 5 -0.0352270194386648 1106 6 0.01678438346552724 1106 7 0.0011934143829321897 1106 8 0.022834775475693903 1106 9 -0.09543889395081351 1106 10-11 0.022240354658570993 1106 12-13 0.07042856252493124 1106 14-15 0.01196990249398766 1106 16-17 -0.030396001725627286 1106 18-19 0.10682088252018929 1106 20-21 0.0854882408326958 1106 22-23 0.08181167858248273 1106 24-25 0.12888026471812708 1106 26-27 0.04063572290831985 1106 28-29 0.09049023762794661 1106 30-31 0.06767517670409973 1106 32-33 -0.0030395927745558993 1106 34-35 0.13071746315753074 1106 36-37 0.13862933238976183 1106 38-39 0.2221270261222159 1106 40-41 0.14164965050019163 1106 42-43 0.05169157164772287 1106 44-45 -0.08047114365817976 1106 46-47 -0.062453930342982744 1106 48-49 -0.04833197572285286 1106 50-51 -0.04890237868540481 1106 52-53 0.0428965540587356 1106 54-55 0.13391708723487028 1106 56-57 0.12429609534494546 1106 58-59 0.08164324745403206 1106 60-61 0.06609123587089982 1106 62-63 0.021154837823509354 1106 64-65 0.19746357838251782 1106 66-67 0.12306593569282853 1106 68-69 0.14649334890015098 1106 70-71 0.11129910258195963 1106 72-73 0.015631665688502494 1106 74-75 0.19727243548967976 1106 76-77 -0.01588835899070773 1106 78-79 -0.16068641646501192 1106 80-81 4.6761879514889415E-4 1106 82-83 0.09634589513307645 1106 84-85 0.12828520859194725 1106 86-87 0.09402091418404623 1106 88-89 0.18050074944964578 1106 90-91 0.4024011285693234 1106 92-93 0.4794899538444497 1106 94-95 0.4681550862949244 1106 96-97 0.4531482693664728 1106 98-99 0.34734728997646513 1106 100-101 0.540411017261814 1107 1 0.07474121284337798 1107 2 0.14265641056128686 1107 3 0.15414864842669118 1107 4 0.13242162679300407 1107 5 0.12025837407131945 1107 6 0.1573104887012562 1107 7 0.17531013417578833 1107 8 0.1696249995232293 1107 9 0.20720282634669474 1107 10-11 0.14221653504170462 1107 12-13 0.13694479674993687 1107 14-15 0.2818511622544264 1107 16-17 0.22379559938203641 1107 18-19 0.26895530737186135 1107 20-21 0.24768368280771114 1107 22-23 0.238562145157033 1107 24-25 0.20813967282450108 1107 26-27 0.29980641303492206 1107 28-29 0.40727829625576817 1107 30-31 0.18858611189014596 1107 32-33 0.17712240998176298 1107 34-35 0.23410111497272368 1107 36-37 0.29956566537993723 1107 38-39 0.3221707932918818 1107 40-41 0.3111806811482225 1107 42-43 0.23773415701205636 1107 44-45 0.2260848480398181 1107 46-47 0.3365530962604808 1107 48-49 0.12346838726021048 1107 50-51 0.22828143792190048 1107 52-53 0.2937003284953974 1107 54-55 0.30043969843742246 1107 56-57 0.3173278939829345 1107 58-59 0.42482946987934156 1107 60-61 0.4241475147622751 1107 62-63 0.3324075423042743 1107 64-65 0.39514804736838727 1107 66-67 0.3244488222707105 1107 68-69 0.36446027670175596 1107 70-71 0.4754690219659494 1107 72-73 0.4891300480465688 1107 74-75 0.42445914238177807 1107 76-77 0.40218628926622557 1107 78-79 0.31811380338014317 1107 80-81 0.5091593474423703 1107 82-83 0.5373992250164221 1107 84-85 0.4480009422012827 1107 86-87 0.4145404222300506 1107 88-89 0.5964164008464259 1107 90-91 0.4842709684934192 1107 92-93 0.6049468941063907 1107 94-95 0.6615194970713745 1107 96-97 0.4635897672496867 1107 98-99 0.62844537798426 1107 100-101 0.6956089880066294 1108 1 -0.1514560899489581 1108 2 -0.1321395807576664 1108 3 -0.032489579401278945 1108 4 0.009543609285891819 1108 5 -0.0866475795855024 1108 6 -0.06334471191075863 1108 7 -4.2470792757853815E-4 1108 8 -0.02211020465681912 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-0.726999469410174 2316 74-75 -0.6240186759962327 2316 76-77 -0.515755115610709 2316 78-79 -0.7452799398247691 2316 80-81 -0.7943316728223238 2316 82-83 -0.858835417061222 2316 84-85 -0.6258242533746561 2316 86-87 -0.6920382863556718 2316 88-89 -0.6487267762713884 2316 90-91 -0.7738107115386228 2316 92-93 -0.619714802438228 2316 94-95 -0.5787626064019911 2316 96-97 -0.5000858341923049 2316 98-99 -0.7476619864613845 2316 100-101 -0.6908598547233424 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 12.0 5 32.0 6 68.0 7 156.0 8 369.0 9 649.0 10 1080.0 11 1794.0 12 2654.0 13 3788.0 14 4832.0 15 6309.0 16 7895.0 17 10407.0 18 10202.0 19 7406.0 20 6872.0 21 7135.0 22 7851.0 23 8977.0 24 10729.0 25 13179.0 26 16546.0 27 20568.0 28 26048.0 29 32540.0 30 40475.0 31 50187.0 32 63932.0 33 82920.0 34 115378.0 35 173025.0 36 298721.0 37 555717.0 38 606510.0 39 114234.0 40 66.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.69513659164138 17.979366779568586 12.917927785899403 24.40756884289063 2 16.072188087155155 19.224169071232613 39.50867159678483 25.194971244827403 3 20.35191982248982 22.85240049262193 28.246775179753307 28.548904505134942 4 13.036596751535978 15.177917234665756 35.53650581394342 36.24898019985485 5 14.326438242981784 36.951667968662036 33.72264727126597 14.999246517090207 6 34.87573594494551 34.457596233624734 16.477545343215798 14.18912247821395 7 30.677666939768134 31.10243210426125 19.419549677041637 18.800351278928982 8 27.424829253844063 35.4175074785344 18.529487163942292 18.628176103679248 9 26.811953183595204 15.759701308981688 18.92108175895822 38.50726374846489 10-11 26.215255223733237 25.782195916628126 26.335747951298856 21.666800908339788 12-13 26.41757694514512 22.565220686146084 27.265397148455683 23.75180522025311 14-15 23.046054261162375 24.30489036390626 24.911574017137838 27.737481357793527 16-17 23.392396961625387 27.553316840322513 25.53829991499445 23.515986283057654 18-19 23.333308794617878 26.42815323937026 27.238493796324043 23.00004416968782 20-21 23.99341265258303 25.598093428003715 27.325756498087824 23.082737421325433 22-23 23.471279195574542 25.515470111729847 27.23183093571988 23.781419756975726 24-25 23.369465193330928 25.669282404891337 27.504333701277528 23.45691870050021 26-27 23.293134429703812 25.95673311246563 27.26780039454787 23.48233206328269 28-29 23.12513586756474 26.18145237771486 27.311567385548628 23.38184436917177 30-31 23.350911171472156 25.975873145097815 27.187477333033094 23.485738350396936 32-33 23.06904005267519 25.875691486378784 27.262334272239887 23.792934188706145 34-35 23.452439645173225 26.036861919259568 27.188691341889832 23.322007093677378 36-37 23.616133735595575 25.782205048948303 27.125268428635334 23.47639278682079 38-39 23.28827659434883 26.02578256754776 27.175494803885293 23.51044603421812 40-41 23.585987032215066 25.89835016483628 27.198441406146816 23.317221396801834 42-43 23.461680119019597 25.874918992490688 27.033199167070666 23.630201721419045 44-45 23.354448263729125 26.086339892278126 27.07683979754784 23.482372046444908 46-47 23.608251195498262 26.203061142511537 26.7137423994938 23.4749452624964 48-49 23.637737329745587 26.21098316089721 26.611139767454855 23.540139741902344 50-51 23.642132400116147 26.336636793409852 26.570489633714995 23.450741172759002 52-53 23.745793037472957 26.22985784402143 26.528153492339175 23.496195626166436 54-55 23.661465238249583 26.22294626071145 26.59475214997643 23.52083635106253 56-57 23.45086056505484 26.55640382563817 26.40873424433773 23.584001364969268 58-59 23.499194324720264 26.198477163295504 26.643168345644003 23.659160166340225 60-61 23.40096650574116 26.28907308076466 26.76316711966436 23.546793293829825 62-63 23.39677024314114 26.26985687289003 26.843543171106443 23.489829712862388 64-65 23.45138434196988 26.283422019905977 26.673634278746057 23.591559359378085 66-67 23.28625941637119 26.47520261761695 26.542929472267502 23.69560849374436 68-69 23.487448014009583 26.199856578425447 26.738899984237484 23.573795423327486 70-71 23.50585075655812 26.144840326440256 26.51662383935384 23.83268507764778 72-73 23.464483560120776 26.301338423014425 26.565601998053477 23.668576018811322 74-75 23.41348543872625 26.470958863297415 26.48661312030264 23.628942577673694 76-77 23.535556117175034 26.317351402536183 26.377030100755256 23.770062379533528 78-79 23.485346001344652 26.396942580842886 26.481991301795947 23.63572011601651 80-81 23.474257692855655 26.39093680943578 26.409384333375343 23.725421164333227 82-83 23.540105210464233 26.547562959126587 26.401478212218894 23.51085361819029 84-85 23.45937285172214 26.541763874830167 26.27182920954201 23.727034063905684 86-87 23.426826960449485 26.414180619464556 26.446761655307384 23.712230764778578 88-89 23.56308197004059 26.428250680648247 26.212133156618133 23.796534192693034 90-91 23.311940367551294 26.597506381294885 26.349942762179296 23.740610488974525 92-93 23.365901091990562 26.470847534769632 26.392652776185365 23.77059859705444 94-95 23.29837490510861 26.394529925485628 26.528081572167906 23.779013597237856 96-97 23.440647370247827 26.241766969315822 26.431593012271033 23.88599264816532 98-99 23.212731769728723 26.301031670712412 26.294146215457154 24.192090344101707 100-101 23.20009284367533 26.382933392457662 26.30788764183127 24.109086122035738 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 77.0 1 60.0 2 82.0 3 129.5 4 109.0 5 102.5 6 221.0 7 443.5 8 708.0 9 813.5 10 713.5 11 648.5 12 691.0 13 781.5 14 894.5 15 1020.5 16 1170.0 17 1278.5 18 1359.0 19 1519.5 20 1750.0 21 2082.0 22 2618.0 23 3386.0 24 4468.5 25 5791.0 26 7853.0 27 10544.0 28 12986.0 29 15740.0 30 19191.5 31 23168.5 32 28240.0 33 31487.0 34 33890.0 35 37718.0 36 42623.0 37 47840.5 38 51991.0 39 55609.5 40 60032.0 41 64642.0 42 67978.0 43 73061.5 44 78046.5 45 82639.0 46 87657.0 47 90586.5 48 93500.0 49 94814.0 50 91720.5 51 89002.0 52 88921.5 53 89398.5 54 90152.5 55 86440.0 56 79257.0 57 71825.0 58 66229.0 59 60728.0 60 52818.5 61 45741.5 62 37889.5 63 28306.0 64 20726.0 65 16082.0 66 11615.5 67 8822.5 68 7092.5 69 5548.5 70 4299.0 71 2905.0 72 2282.0 73 2030.0 74 1596.0 75 1176.5 76 799.5 77 495.5 78 302.5 79 163.0 80 86.0 81 42.5 82 25.0 83 14.5 84 8.0 85 7.0 86 5.5 87 2.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26402214373682487 2 0.02052591374959078 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.4642892404372627E-4 12-13 4.3303615505465784E-5 14-15 0.0 16-17 6.495542325819868E-4 18-19 0.0 20-21 2.1651807752732892E-5 22-23 2.1651807752732892E-5 24-25 0.0021218771597678234 26-27 0.002186832583026022 28-29 0.0018187518512295627 30-31 0.001017634964378446 32-33 0.0012774566574112406 34-35 0.0015805819659495011 36-37 1.2991084651639735E-4 38-39 1.0825903876366446E-4 40-41 6.495542325819868E-4 42-43 0.0033127265861681325 44-45 0.003832369972233722 46-47 0.013056040074897933 48-49 0.013207602729167064 50-51 0.005694425438968751 52-53 0.00682031944211086 54-55 2.814735007855276E-4 56-57 0.002879690431113474 58-59 0.0014290193116803709 60-61 0.0012774566574112406 62-63 0.0 64-65 8.660723101093157E-4 66-67 0.0 68-69 0.0 70-71 8.660723101093157E-5 72-73 0.0012341530419057746 74-75 0.0 76-77 0.19198657934348254 78-79 0.10232644343941563 80-81 9.95983156625713E-4 82-83 0.0 84-85 5.84598809323788E-4 86-87 0.11776418236711421 88-89 0.5760679970692113 90-91 0.30483580135072635 92-93 0.289787794962577 94-95 0.0024683060838115493 96-97 0.002446654276058817 98-99 0.00261986873808068 100-101 7.79465079098384E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2309276.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.55223344483233 #Duplication Level Percentage of deduplicated Percentage of total 1 69.55161099284159 34.46437664381453 2 15.485556498609332 15.3468782128446 3 6.140032717453834 9.12757003722542 4 2.9272332694438417 5.802037852598441 5 1.625976395364747 4.028538095945048 6 1.0216369958567508 3.0374636948742553 7 0.6816356040732935 2.36435966041444 8 0.4578641846329855 1.8150554370369207 9 0.34727439341012184 1.5487399630503813 >10 1.6219634435584416 14.03396434277647 >50 0.09187656234231166 3.1679442481229363 >100 0.04479948763914491 4.046848502465519 >500 0.0018389155258152939 0.5885203445602114 >1k 7.005392479292247E-4 0.627702964270741 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3757 0.16269168345403495 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2832 0.1226358391114791 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.660723101093157E-5 2 0.0 0.0 0.0 0.0 8.660723101093157E-5 3 0.0 0.0 0.0 0.0 8.660723101093157E-5 4 0.0 0.0 0.0 4.3303615505465784E-5 8.660723101093157E-5 5 0.0 0.0 0.0 4.3303615505465784E-5 8.660723101093157E-5 6 0.0 0.0 0.0 4.3303615505465784E-5 8.660723101093157E-5 7 0.0 0.0 0.0 8.660723101093157E-5 8.660723101093157E-5 8 0.0 0.0 0.0 8.660723101093157E-5 8.660723101093157E-5 9 0.0 0.0 0.0 8.660723101093157E-5 8.660723101093157E-5 10-11 0.0 0.0 0.0 8.660723101093157E-5 8.660723101093157E-5 12-13 0.0 0.0 0.0 1.0825903876366446E-4 5.845988093237881E-4 14-15 0.0 0.0 0.0 1.5156265426913024E-4 6.712060403347197E-4 16-17 0.0 0.0 0.0 2.598216930327947E-4 8.660723101093157E-4 18-19 0.0 0.0 0.0 3.247771162909934E-4 9.093759256147815E-4 20-21 0.0 0.0 0.0 5.629470015710552E-4 0.001407367503927638 22-23 0.0 0.0 0.0 9.093759256147815E-4 0.0015589301581967682 24-25 0.0 0.0 0.0 0.001385715696174905 0.001602233773702234 26-27 0.0 0.0 0.0 0.001602233773702234 0.001970314505498693 28-29 0.0 0.0 0.0 0.002035269928756892 0.002013618121004159 30-31 0.0 0.0 0.0 0.003615851894706393 0.0021218771597678234 32-33 0.0 0.0 0.0 0.006473890518067135 0.002208484390778755 34-35 0.0 0.0 0.0 0.009981483374009864 0.0022517880062842208 36-37 0.0 0.0 0.0 0.016000685929269606 0.0022734398140369537 38-39 0.0 0.0 0.0 0.02780092115450903 0.0023167434295424194 40-41 0.0 0.0 0.0 0.04215606969457094 0.0023383952372951523 42-43 0.0 0.0 0.0 0.05781032669979682 0.0023383952372951523 44-45 0.0 0.0 0.0 0.074655433131423 0.0024683060838115497 46-47 0.0 0.0 0.0 0.09565768665157391 0.002598216930327947 48-49 0.0 0.0 0.0 0.11878181733149264 0.0026631723535861457 50-51 0.0 0.0 0.0 0.14491554928904124 0.002814735007855276 52-53 0.0 0.0 0.0 0.1714173619783863 0.003204467547404468 54-55 0.0 0.0 0.0 0.19928323855615354 0.0033343783939208654 56-57 0.0 0.0 0.0 0.2324321562255876 0.003420985624931797 58-59 0.0 0.0 0.0 0.2703661234083756 0.0035292446636954614 60-61 0.0 0.0 0.0 0.3128686220269903 0.00359420008695366 62-63 0.0 0.0 0.0 0.3541586194114519 0.003767414548975523 64-65 0.0 0.0 0.0 0.40198746273723884 0.003940629010997386 66-67 0.0 0.0 0.0 0.4562685447733402 0.003983932626502852 68-69 0.0 0.0 0.0 0.5210940571850224 0.004048888049761051 70-71 0.0 0.0 0.0 0.5972001614358786 0.004092191665266517 72-73 0.0 0.0 0.0 0.6718772463750544 0.00441696878155751 74-75 0.0 0.0 0.0 0.751252773596573 0.004806701321106702 76-77 0.0 0.0 0.0 0.8316892393979758 0.004958263975375832 78-79 0.0 0.0 0.0 0.9314174659070635 0.005066523014139497 80-81 0.0 0.0 0.0 1.0431840975266709 0.005218085668408627 82-83 0.0 0.0 0.0 1.1653435968675896 0.005412951938183223 84-85 0.0 0.0 0.0 1.2877845697092942 0.0054995591691941546 86-87 0.0 0.0 0.0 1.4171324692241205 0.005737729054474216 88-89 0.0 0.0 0.0 1.567049586104043 0.006019202555259744 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3900 0.0 35.497726 1 GTATCAA 5970 0.0 33.549026 1 TCAACGC 7255 0.0 27.396578 4 CAACGCA 7370 0.0 27.033453 5 ATCAACG 7360 0.0 27.005732 3 TATCAAC 7665 0.0 26.369493 2 AACGCAG 7685 0.0 26.048836 6 TACATGG 8775 0.0 25.25074 2 GTACATG 9265 0.0 24.852615 1 ATAACGC 115 1.293856E-4 24.749882 3 ACATGGG 8695 0.0 23.950504 3 GTACCTG 2080 0.0 21.728636 1 ACGCAGA 9310 0.0 21.349321 7 CGCAGAG 9365 0.0 21.173285 8 GTACACG 320 1.8189894E-11 20.813745 1 GTGTAGC 1540 0.0 20.388975 1 CATGGGG 5050 0.0 20.102133 4 GAGTACA 7250 0.0 19.751526 1 CCTATAC 520 0.0 19.157362 3 AGTACAT 6530 0.0 18.891417 2 >>END_MODULE