FastQCFastQC Report
Thu 26 May 2016
SRR936297_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936297_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2297687
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44830.19510925552523037No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31030.1350488556535333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61350.039.817711
GGTATCA39400.036.8878481
TCAACGC77900.030.9221884
ATCAACG78450.030.7663943
CAACGCA78200.030.7430445
TATCAAC81400.029.8251632
AACGCAG82300.029.3252226
GTACATG102800.028.4140551
TACATGG102950.027.6272742
ACATGGG100300.027.366953
TACCTGG21450.026.2548522
GTACCTG23250.025.6559891
ACGCAGA95250.025.0406727
CGCAGAG98350.024.3476268
CATGGGG58650.024.0457384
ACCGTAC1000.001690295423.6619458
GAGTACA76350.021.0819631
CATGGGA40350.020.9942044
AGTACAT71450.020.864032
GCAGAGT112000.020.788719
Produced by FastQC (version 0.11.5)