##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936297_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2297687 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.701700013970573 33.0 31.0 34.0 30.0 34.0 2 31.792298951075583 34.0 31.0 34.0 30.0 34.0 3 31.863169787703896 34.0 31.0 34.0 30.0 34.0 4 35.34930258124801 37.0 35.0 37.0 33.0 37.0 5 35.33688052376151 37.0 35.0 37.0 33.0 37.0 6 35.425352974534825 37.0 35.0 37.0 33.0 37.0 7 35.366092074333885 37.0 35.0 37.0 33.0 37.0 8 35.3670817652709 37.0 35.0 37.0 33.0 37.0 9 37.03474711742722 39.0 37.0 39.0 33.0 39.0 10-11 36.95389189215067 39.0 37.0 39.0 33.0 39.0 12-13 36.855610664115694 39.0 37.0 39.0 33.0 39.0 14-15 38.10112408696224 40.0 38.0 41.0 33.0 41.0 16-17 38.04989452436298 40.0 38.0 41.0 33.0 41.0 18-19 37.99972994581072 40.0 38.0 41.0 33.0 41.0 20-21 37.916176137132695 40.0 38.0 41.0 33.0 41.0 22-23 37.75480385274409 40.0 38.0 41.0 32.5 41.0 24-25 37.90718361552292 40.0 38.0 41.0 33.0 41.0 26-27 37.86287079136541 40.0 38.0 41.0 33.0 41.0 28-29 37.73415656701718 40.0 38.0 41.0 32.5 41.0 30-31 37.5822150710693 40.0 38.0 41.0 32.0 41.0 32-33 37.40958080887432 40.0 37.0 41.0 32.0 41.0 34-35 37.15102405157883 40.0 37.0 41.0 31.0 41.0 36-37 36.95251028534348 40.0 37.0 41.0 31.0 41.0 38-39 36.79513636974923 40.0 37.0 41.0 30.0 41.0 40-41 36.61627845742262 40.0 36.0 41.0 30.0 41.0 42-43 36.43225208655487 40.0 36.0 41.0 30.0 41.0 44-45 36.24559633231158 40.0 36.0 41.0 29.5 41.0 46-47 35.97274759355822 39.0 35.0 41.0 28.5 41.0 48-49 35.80143879475315 39.0 35.0 41.0 28.0 41.0 50-51 35.48668421765018 38.5 35.0 40.5 27.5 41.0 52-53 35.49787351366831 39.0 35.0 40.5 28.0 41.0 54-55 35.56664528284314 39.0 35.0 41.0 28.0 41.0 56-57 35.39477330898421 39.0 35.0 41.0 27.5 41.0 58-59 35.15639292906301 38.5 35.0 41.0 27.0 41.0 60-61 34.85370004704731 38.0 34.5 40.0 26.0 41.0 62-63 34.540223494322774 37.5 34.0 40.0 26.0 41.0 64-65 34.20413224255523 37.0 34.0 40.0 26.0 41.0 66-67 33.84260606427246 36.5 34.0 39.5 25.5 41.0 68-69 33.458521547974115 36.0 33.5 39.0 24.5 41.0 70-71 33.04793755633382 35.5 33.0 39.0 23.5 40.5 72-73 32.65271575284188 35.0 33.0 37.5 22.5 40.0 74-75 32.240754506597284 35.0 33.0 37.0 21.5 39.0 76-77 31.85668674628006 35.0 32.0 36.5 20.0 39.0 78-79 31.472455995964637 35.0 32.0 36.0 19.0 39.0 80-81 31.089549403378268 35.0 32.0 36.0 18.0 37.0 82-83 30.751260724371946 35.0 31.0 35.0 15.5 37.0 84-85 30.443734285827443 35.0 31.0 35.0 9.5 36.5 86-87 30.138776517428177 34.0 31.0 35.0 7.0 36.0 88-89 29.826777972804827 34.0 31.0 35.0 2.0 36.0 90-91 29.487334219151695 34.0 30.0 35.0 2.0 35.5 92-93 29.187702023817863 34.0 30.0 35.0 2.0 35.0 94-95 28.827181422012657 34.0 29.5 35.0 2.0 35.0 96-97 28.414299467246845 34.0 29.0 35.0 2.0 35.0 98-99 27.921666005857194 34.0 29.0 35.0 2.0 35.0 100-101 24.6653140745454 30.0 22.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1764216576686195 1101 2 0.3005733870465903 1101 3 0.33544827644543673 1101 4 0.09469412208277816 1101 5 0.3469848087883918 1101 6 0.2508677514518567 1101 7 0.40787495932281814 1101 8 0.29381611248500406 1101 9 0.42203342987014025 1101 10-11 0.5250870077491854 1101 12-13 0.47272206517332194 1101 14-15 0.5720787906232161 1101 16-17 0.620269341760249 1101 18-19 0.6180404450984867 1101 20-21 0.731275191421382 1101 22-23 0.6280102881781531 1101 24-25 0.45624538939308223 1101 26-27 0.48940829464274316 1101 28-29 0.39607639171023834 1101 30-31 0.6292292225906593 1101 32-33 0.6685512688607318 1101 34-35 0.38091554664890737 1101 36-37 0.3722573268631635 1101 38-39 0.4140963648008906 1101 40-41 0.35936753372616437 1101 42-43 0.33315627540218173 1101 44-45 0.34707650285763947 1101 46-47 0.26124195042393694 1101 48-49 0.2926291807622192 1101 50-51 0.378136638735576 1101 52-53 0.3923678716343204 1101 54-55 0.26708467293017435 1101 56-57 0.27150978815764404 1101 58-59 0.16543805175147241 1101 60-61 0.10379948381579851 1101 62-63 0.2552060563880758 1101 64-65 0.2343525552846799 1101 66-67 0.2905836375536026 1101 68-69 0.18544449658341478 1101 70-71 0.32532749550704665 1101 72-73 0.341118188862751 1101 74-75 0.27003016029089366 1101 76-77 0.2778173761469276 1101 78-79 0.3639138351678639 1101 80-81 0.364259292019657 1101 82-83 0.4659282240811997 1101 84-85 0.4384267343602879 1101 86-87 0.3605530182428147 1101 88-89 0.4227851595809149 1101 90-91 0.3975668033486883 1101 92-93 0.4043068237051344 1101 94-95 0.5795156702815056 1101 96-97 0.5699854705867118 1101 98-99 0.5058235996476341 1101 100-101 0.8354997986140198 1102 1 0.308506820058593 1102 2 0.35154449950804434 1102 3 0.2537916238901339 1102 4 0.2289275067683718 1102 5 0.23650289596285035 1102 6 0.2492911277412304 1102 7 0.2400566908605839 1102 8 0.19493056583555557 1102 9 0.32393632114538207 1102 10-11 0.44049609029185177 1102 12-13 0.39488657563330065 1102 14-15 0.4974788718119001 1102 16-17 0.47917765654037225 1102 18-19 0.40628888751125913 1102 20-21 0.3438571680380349 1102 22-23 0.3970374359591986 1102 24-25 0.5193126070670004 1102 26-27 0.4574116329600173 1102 28-29 0.43063264008709723 1102 30-31 0.40277091499649487 1102 32-33 0.22729695413607942 1102 34-35 0.1680332727606526 1102 36-37 0.1983143556095044 1102 38-39 0.23480918630934156 1102 40-41 0.2479035404028025 1102 42-43 0.22201527183946013 1102 44-45 0.26252920540701297 1102 46-47 0.29164080873960785 1102 48-49 0.17120356295920658 1102 50-51 0.11303756080705085 1102 52-53 0.28301944863623874 1102 54-55 0.2880365219898309 1102 56-57 0.31202016744999383 1102 58-59 0.2631786362595605 1102 60-61 0.3102301570010937 1102 62-63 0.5067852014863661 1102 64-65 0.25289559663291783 1102 66-67 0.20671711477412913 1102 68-69 0.27340600507923085 1102 70-71 0.3143527774612238 1102 72-73 0.273514742630077 1102 74-75 0.30700883187258654 1102 76-77 0.2900265655964347 1102 78-79 0.19769286579516177 1102 80-81 0.2209617707958742 1102 82-83 0.13055971066615868 1102 84-85 0.27190458523029193 1102 86-87 0.10507139537685362 1102 88-89 0.0487814228843888 1102 90-91 0.16004729411158536 1102 92-93 0.18753883927722725 1102 94-95 0.13923070304694107 1102 96-97 0.2655697643586308 1102 98-99 0.2100232229523158 1102 100-101 0.20362542423334062 1103 1 0.20945535007984262 1103 2 0.2718643482062504 1103 3 0.2568337964206826 1103 4 0.1658116844793156 1103 5 0.14518205110947946 1103 6 0.18098117290909244 1103 7 0.25393861091410486 1103 8 0.16243486607701385 1103 9 0.32616798338060704 1103 10-11 0.2520301300075971 1103 12-13 0.3407469402820098 1103 14-15 0.4016734894349767 1103 16-17 0.47413692012126063 1103 18-19 0.34422685122491714 1103 20-21 0.48340116832447677 1103 22-23 0.5073817299422032 1103 24-25 0.4421212496326987 1103 26-27 0.3984041537034386 1103 28-29 0.42332529507358174 1103 30-31 0.3997475031429829 1103 32-33 0.3971254692861237 1103 34-35 0.2178551321620006 1103 36-37 0.1416566204965264 1103 38-39 0.1527109921900447 1103 40-41 0.14247711879716718 1103 42-43 0.14120040612422002 1103 44-45 0.25866744878964454 1103 46-47 0.3535775286081204 1103 48-49 0.38922899311823755 1103 50-51 0.14692486137369798 1103 52-53 0.1794061912873275 1103 54-55 0.22959590946475572 1103 56-57 0.2000341302984623 1103 58-59 0.2170234469582013 1103 60-61 0.18221502191686767 1103 62-63 0.20115672861528822 1103 64-65 0.27696444169621515 1103 66-67 0.366068343586214 1103 68-69 0.32306392313945764 1103 70-71 0.2956979204568171 1103 72-73 0.3240791815092692 1103 74-75 0.346947002566786 1103 76-77 0.1781339639736501 1103 78-79 0.3786433969552938 1103 80-81 0.2754437578483113 1103 82-83 0.23787200947935716 1103 84-85 0.33836091160035053 1103 86-87 0.36995462058492734 1103 88-89 0.3811903330533646 1103 90-91 0.513552892150468 1103 92-93 0.45236136817725026 1103 94-95 0.32571563393162606 1103 96-97 0.5031512997324405 1103 98-99 0.6841897706787421 1103 100-101 0.8019815229738505 1104 1 0.22991116687166624 1104 2 0.15467530317830835 1104 3 0.21971498571432235 1104 4 0.27763144840611176 1104 5 0.20016738835288095 1104 6 0.17745250566608917 1104 7 0.2563470887561081 1104 8 0.18225783942413187 1104 9 0.35497366089036575 1104 10-11 0.26686381083978716 1104 12-13 0.2977305372059291 1104 14-15 0.4988968888213421 1104 16-17 0.4952962102065257 1104 18-19 0.2813684047425653 1104 20-21 0.4399016570830341 1104 22-23 0.47141347947179923 1104 24-25 0.4408463808629577 1104 26-27 0.512583660876615 1104 28-29 0.5564604410513567 1104 30-31 0.5168334285917666 1104 32-33 0.634092849596783 1104 34-35 0.6379650039547045 1104 36-37 0.6107002640926638 1104 38-39 0.4714239468343777 1104 40-41 0.5203814181773225 1104 42-43 0.585669806448557 1104 44-45 0.6982746903267767 1104 46-47 0.632276555046289 1104 48-49 0.7146574337806868 1104 50-51 0.750735906751963 1104 52-53 0.6707716922789686 1104 54-55 0.777136860651062 1104 56-57 0.6299551943795052 1104 58-59 0.6254638551422218 1104 60-61 0.6264880712616971 1104 62-63 0.7544292920267708 1104 64-65 0.6971399992482432 1104 66-67 0.7122993935977959 1104 68-69 0.5747230182863419 1104 70-71 0.6071925045600182 1104 72-73 0.5582672772720443 1104 74-75 0.5214062604448415 1104 76-77 0.5711106730747275 1104 78-79 0.4808949352734011 1104 80-81 0.48919369428144677 1104 82-83 0.65740342281088 1104 84-85 0.514042772160078 1104 86-87 0.5512981698195816 1104 88-89 0.4444626704493224 1104 90-91 0.47562254448209984 1104 92-93 0.500710324833129 1104 94-95 0.2470714162560803 1104 96-97 0.5525250954935181 1104 98-99 0.7799737337478092 1104 100-101 0.8454541948727723 1105 1 0.1059412385355678 1105 2 0.18131286294001825 1105 3 0.12465317457546021 1105 4 -0.04226159830810161 1105 5 0.09435416860213053 1105 6 0.014317513275649674 1105 7 0.19541202943437952 1105 8 0.09966813644769701 1105 9 0.10077974069544382 1105 10-11 0.17289692815101176 1105 12-13 0.18868450872091813 1105 14-15 0.11832433448069679 1105 16-17 0.19148331508165484 1105 18-19 0.22009675123874928 1105 20-21 0.22626385932313298 1105 22-23 0.3518698843131105 1105 24-25 0.16988076932159402 1105 26-27 0.10462683885457125 1105 28-29 0.11931099824980151 1105 30-31 0.28820334069657605 1105 32-33 0.2919711367757927 1105 34-35 0.02301244857453355 1105 36-37 0.18157961268888556 1105 38-39 0.32460596438424005 1105 40-41 0.23558273181790668 1105 42-43 0.16956667572371487 1105 44-45 0.2495311566281444 1105 46-47 0.1746266686197515 1105 48-49 0.25308753024746267 1105 50-51 0.2960065134284946 1105 52-53 0.299756566171844 1105 54-55 0.31916155045552586 1105 56-57 0.41146465002020705 1105 58-59 0.41918929733100896 1105 60-61 0.24723043411866286 1105 62-63 0.08165503102938487 1105 64-65 0.1703239408447601 1105 66-67 0.1945187175917482 1105 68-69 0.18336155466131032 1105 70-71 0.23243722779366038 1105 72-73 0.32309622861189524 1105 74-75 0.32830663495003876 1105 76-77 0.3538464804949797 1105 78-79 0.4029985155503759 1105 80-81 0.36342145398899106 1105 82-83 0.33887316649321164 1105 84-85 0.44007161279886375 1105 86-87 0.5377087923808972 1105 88-89 0.4374717016143208 1105 90-91 0.42812446624908773 1105 92-93 0.34147563035076445 1105 94-95 0.35850027622651837 1105 96-97 0.31668655765955833 1105 98-99 0.4619352035735602 1105 100-101 0.3963844395184992 1106 1 0.15241076137401066 1106 2 0.13463035086661534 1106 3 0.21263392717828467 1106 4 0.15970604571574398 1106 5 0.1806638422535869 1106 6 0.21256058193173288 1106 7 0.24407120419193973 1106 8 0.19150808762434934 1106 9 0.2835356681661523 1106 10-11 0.14458621516461534 1106 12-13 0.1877707126661079 1106 14-15 0.17459902361053992 1106 16-17 0.18646340179213894 1106 18-19 0.1784649078377143 1106 20-21 0.21333674804818514 1106 22-23 0.2285237249271077 1106 24-25 0.26732925460257206 1106 26-27 0.18539539945449945 1106 28-29 0.17332642541251175 1106 30-31 0.2057013147814999 1106 32-33 0.1780002250198791 1106 34-35 0.05772838047576556 1106 36-37 0.17221804627892823 1106 38-39 0.2783535898716707 1106 40-41 0.20373832384698431 1106 42-43 0.2676780982072344 1106 44-45 0.2282859729648834 1106 46-47 0.3280947156376186 1106 48-49 0.3440913946996247 1106 50-51 0.2865696455829507 1106 52-53 0.24062534772895106 1106 54-55 0.2910146837613965 1106 56-57 0.37177127133672627 1106 58-59 0.30027946510872994 1106 60-61 0.42535499495149764 1106 62-63 0.3804488289930035 1106 64-65 0.2907787658936911 1106 66-67 0.31138096368471935 1106 68-69 0.3530136094930114 1106 70-71 0.47949193472440754 1106 72-73 0.4847828581853122 1106 74-75 0.37119277261244576 1106 76-77 0.4134574172404655 1106 78-79 0.42344460650837945 1106 80-81 0.5031249896102921 1106 82-83 0.4210646834362528 1106 84-85 0.41713061453823386 1106 86-87 0.40193778667330804 1106 88-89 0.23430459765937428 1106 90-91 0.2729541470979626 1106 92-93 0.3440696991368739 1106 94-95 0.38695852749077275 1106 96-97 0.39954352914964986 1106 98-99 0.3627946249870959 1106 100-101 0.3342651331497919 1107 1 0.1093326709666691 1107 2 0.2439652363762903 1107 3 0.34175386398253593 1107 4 0.1318292358538784 1107 5 0.12440611168783988 1107 6 0.3345502086818257 1107 7 0.23700325836566094 1107 8 0.35903399476401177 1107 9 0.2956811015455685 1107 10-11 0.37672146238681137 1107 12-13 0.25684689057045773 1107 14-15 0.39871527053130507 1107 16-17 0.3053114305628313 1107 18-19 0.3833573815148199 1107 20-21 0.5102892161125041 1107 22-23 0.3738583609798738 1107 24-25 0.5523207834616173 1107 26-27 0.430728476401562 1107 28-29 0.5648802301501163 1107 30-31 0.5189230507937239 1107 32-33 0.6033450472298583 1107 34-35 0.3903375958298412 1107 36-37 0.3877929924897856 1107 38-39 0.36154746986210995 1107 40-41 0.4896523235767418 1107 42-43 0.25031485776195694 1107 44-45 0.44255646740926835 1107 46-47 0.7314977236722058 1107 48-49 0.6800137096597609 1107 50-51 0.592593510294158 1107 52-53 0.5757109426317157 1107 54-55 0.7513952094918395 1107 56-57 0.7402477132496443 1107 58-59 0.7490446533967301 1107 60-61 0.7759588885581294 1107 62-63 0.8253318440955439 1107 64-65 0.6418607852473954 1107 66-67 0.6150713668694507 1107 68-69 0.6286972773411392 1107 70-71 0.7451713410169631 1107 72-73 0.6987441299998025 1107 74-75 0.5422682437222193 1107 76-77 0.5574096462419078 1107 78-79 0.6038799088189784 1107 80-81 0.6438160891955711 1107 82-83 0.8382293977561197 1107 84-85 0.7057964358231494 1107 86-87 0.4077818345274622 1107 88-89 0.6253257079505126 1107 90-91 0.7780498242175682 1107 92-93 0.4622681005338336 1107 94-95 0.7203213105983828 1107 96-97 0.7459456329029344 1107 98-99 0.8819482384744006 1107 100-101 0.9172355413236666 1108 1 0.07372266448204456 1108 2 0.07919737889193712 1108 3 0.1318064015907332 1108 4 0.07304539803549659 1108 5 0.1577593219234572 1108 6 0.08750914346530436 1108 7 0.05568831435419952 1108 8 0.07860929548184004 1108 9 0.1374923973051807 1108 10-11 0.07147754584894273 1108 12-13 -0.0011868477185359438 1108 14-15 -0.017012350133946086 1108 16-17 0.1939647762279577 1108 18-19 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-1.4523949254017054 2316 54-55 -1.5192708334151064 2316 56-57 -1.4873378398127102 2316 58-59 -1.5525161259165259 2316 60-61 -1.4740899634203757 2316 62-63 -1.4513649341578798 2316 64-65 -1.3697118344606523 2316 66-67 -1.3737260464596872 2316 68-69 -1.3866464667076173 2316 70-71 -1.3033966336131293 2316 72-73 -1.3721949871869867 2316 74-75 -1.2770825217634467 2316 76-77 -1.5664088396779583 2316 78-79 -1.6891659706408397 2316 80-81 -1.4748685505831887 2316 82-83 -1.4585202896740164 2316 84-85 -1.4451888009433453 2316 86-87 -1.5965519983656549 2316 88-89 -1.6082735236658259 2316 90-91 -1.6444475054836083 2316 92-93 -1.6031337690893466 2316 94-95 -1.5888371105719123 2316 96-97 -1.7575266663692553 2316 98-99 -1.6960910583883084 2316 100-101 -1.8605005420724616 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20290.0 3 4471.0 4 3692.0 5 3005.0 6 3012.0 7 3281.0 8 3858.0 9 4412.0 10 5314.0 11 6218.0 12 8137.0 13 11417.0 14 11942.0 15 10089.0 16 11631.0 17 14597.0 18 13578.0 19 10487.0 20 10168.0 21 10716.0 22 11298.0 23 12344.0 24 14044.0 25 16703.0 26 19455.0 27 23589.0 28 28056.0 29 34584.0 30 42524.0 31 52465.0 32 66761.0 33 87551.0 34 123262.0 35 184113.0 36 311243.0 37 513449.0 38 494128.0 39 91740.0 40 63.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.66746392837098 17.56091911081154 12.682600579723777 24.089016381093707 2 15.868038537510698 18.85167480776078 40.083825266671255 25.196461388057273 3 20.26756749580729 23.183988780550866 27.90063196454835 28.647811759093493 4 12.912506419909992 14.605839288978647 35.397903844982025 37.083750446129336 5 14.29180792669524 37.22927367716848 33.44529673202386 15.033621664112415 6 35.057881246493544 33.971636731142624 16.57396643118384 14.396515591179993 7 31.111105307850632 31.120593638727012 19.303530399001723 18.464770654420626 8 27.836690741110715 34.877431873588925 18.48635251932898 18.799524865971375 9 27.36878715172704 15.819235845344345 18.91747659027674 37.89450041265187 10-11 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27.24634335480532 23.321752920450376 36-37 23.80527887193985 25.495073264218004 27.21075584096696 23.488892022875184 38-39 23.447522678624917 25.813096208969128 27.188834398483504 23.550546713922458 40-41 23.55996268282986 25.694765492727043 27.32094034720014 23.424331477242955 42-43 23.543815478420097 25.598584088444614 27.168918169837227 23.688682263298062 44-45 23.48719370827072 25.84320234166193 27.139217041784118 23.530386908283234 46-47 23.72481998732333 26.074181713835287 26.743796938789348 23.457201360052036 48-49 23.7293399908484 25.969951940953777 26.78659701181747 23.514111056380358 50-51 23.636391079945103 26.255435469779027 26.575187001160707 23.532986449115164 52-53 23.87285176465236 26.122489332643905 26.539822025542158 23.464836877161577 54-55 23.759952148767468 26.12185224510416 26.50719900798359 23.61099659814478 56-57 23.561072611125656 26.43570174396952 26.46288463628612 23.540341008618704 58-59 23.7023277865275 25.986880806290706 26.61926653052959 23.6915248766522 60-61 23.545876768732153 26.190673519384188 26.771717079829145 23.49173263205451 62-63 23.474493064833908 26.158313780458204 26.847194554205377 23.519998600502507 64-65 23.438588402951392 26.174750724626904 26.70224717995308 23.684413692468624 66-67 23.434211052797636 26.305870900920226 26.528061200505427 23.73185684577671 68-69 23.512252220648016 26.070300508670226 26.807935762941916 23.609511507739846 70-71 23.600488499809213 26.08494954829074 26.636600093807704 23.677961858092335 72-73 23.622195673942514 26.23857893662987 26.412411123947884 23.726814265479735 74-75 23.341600832874025 26.556433339457406 26.406284174518603 23.69568165314996 76-77 23.559675416608897 26.431883280841262 26.327263614005915 23.68117768854393 78-79 23.691393786605307 26.409332994515793 26.41300691966307 23.486266299215835 80-81 23.548893332703503 26.480542565270714 26.338371114505204 23.63219298752058 82-83 23.750845519419556 26.511814965250874 26.29775886234474 23.439580652984834 84-85 23.691278235313284 26.496762188183887 26.17546313578865 23.63649644071418 86-87 23.566180965109048 26.364612741087544 26.418849305673557 23.650356988129847 88-89 23.69153441005092 26.45166582092425 26.184513289821343 23.672286479203485 90-91 23.419618656777317 26.63591814463984 26.278566120078946 23.6658970785039 92-93 23.512863820105366 26.46293127228126 26.306282972760787 23.717921934852594 94-95 23.549301084839495 26.353315730150822 26.40269619989074 23.694686985118942 96-97 23.62609877772934 26.234022002008768 26.419507357542045 23.72037186271985 98-99 23.467973256959624 26.38567804948857 26.221283072192346 23.925065621359455 100-101 23.528124416529693 26.468115137848752 26.057854893148686 23.945905552472865 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 112.0 1 79.5 2 114.5 3 224.0 4 202.0 5 172.0 6 354.0 7 731.0 8 1160.0 9 1364.5 10 1307.0 11 1270.0 12 1480.5 13 1781.0 14 1970.0 15 2162.0 16 2401.0 17 2569.0 18 2635.0 19 2736.0 20 2869.5 21 3025.5 22 3412.0 23 4030.0 24 4846.5 25 5970.0 26 7720.0 27 10026.0 28 12116.0 29 14598.0 30 17533.5 31 21691.5 32 26653.5 33 30126.5 34 33718.5 35 37883.5 36 42645.5 37 47905.5 38 52277.0 39 55875.5 40 58817.5 41 62188.0 42 65953.5 43 70083.0 44 74454.5 45 78495.5 46 81629.5 47 83719.5 48 86191.5 49 88325.0 50 89283.0 51 90125.5 52 90527.0 53 91365.0 54 92178.0 55 88058.5 56 80752.5 57 74282.5 58 69512.0 59 63724.5 60 54698.0 61 46109.5 62 38362.5 63 29637.5 64 22389.5 65 17172.0 66 12386.5 67 9479.5 68 7352.0 69 5462.5 70 4206.0 71 2842.5 72 2103.0 73 1742.0 74 1340.0 75 1026.0 76 695.0 77 389.5 78 231.5 79 132.5 80 80.5 81 62.0 82 47.0 83 39.5 84 33.5 85 22.5 86 17.0 87 19.0 88 19.5 89 18.0 90 22.0 91 22.5 92 17.5 93 14.0 94 21.0 95 25.0 96 22.5 97 22.5 98 17.0 99 13.5 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046829703088366696 2 0.012577866349942356 3 0.010793463165348457 4 0.006397738247202512 5 0.00913962606743216 6 0.006006039987169706 7 0.005570819698244365 8 0.010010066645282844 9 0.015276232141279468 10-11 0.00228490651685804 12-13 0.008834971865184423 14-15 0.008856732879630689 16-17 0.019280258799392606 18-19 0.012099124032124481 20-21 0.0038734605714355348 22-23 0.010075349688621645 24-25 0.02189158053294465 26-27 0.007681638099532268 28-29 0.01838805720709566 30-31 0.015624408372419744 32-33 0.012926042581082629 34-35 0.26870500638250555 36-37 0.47567401478095145 38-39 0.4718658372528547 40-41 0.49353980764133665 42-43 0.48324684780825233 44-45 0.49821842574728414 46-47 0.5047684910956105 48-49 0.5113403174583832 50-51 0.5225254788837644 52-53 0.5109921412272429 54-55 0.4976526393716812 56-57 0.49277817213571734 58-59 0.4903191775032891 60-61 0.4873379185241506 62-63 0.4853576662095403 64-65 0.5009603135675137 66-67 0.4747600521742082 68-69 0.5018089931309182 70-71 0.48285514954821956 72-73 0.49121137909558615 74-75 0.506030629933494 76-77 0.4922341467745606 78-79 0.49197301460120546 80-81 0.494540814305865 82-83 0.494867229522559 84-85 0.4936921347424606 86-87 0.4964340225626902 88-89 0.5103828328227474 90-91 0.533819445381377 92-93 0.5219814535226077 94-95 0.49432320416140235 96-97 0.46629501755461034 98-99 0.45752532873276475 100-101 0.4667302378435357 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2297687.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.39204027270764 #Duplication Level Percentage of deduplicated Percentage of total 1 70.81687175769552 34.26972910085574 2 14.750522603123489 14.276157677076725 3 5.6197203118373285 8.158491949553554 4 2.8200454804313475 5.458710178396037 5 1.609086608287217 3.8933491975254775 6 0.9787369055275987 2.8417845449206083 7 0.6627556346175434 2.245046815296325 8 0.466586667922603 1.8063264659855254 9 0.3506441193364035 1.5271545909883804 >10 1.7419538596012973 15.012222313085639 >50 0.11976990392703123 3.981765843007327 >100 0.05961767373148696 4.943853619203566 >500 0.003148697284079643 1.005393237186037 >1k 5.397766772706798E-4 0.580014466918997 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4483 0.19510925552523037 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3103 0.1350488556535333 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.35220288925341E-5 2 0.0 0.0 0.0 0.0 4.35220288925341E-5 3 0.0 0.0 0.0 0.0 4.35220288925341E-5 4 0.0 0.0 0.0 0.0 4.35220288925341E-5 5 0.0 0.0 0.0 0.0 8.70440577850682E-5 6 0.0 0.0 0.0 0.0 8.70440577850682E-5 7 0.0 0.0 0.0 0.0 8.70440577850682E-5 8 0.0 0.0 0.0 0.0 1.305660866776023E-4 9 0.0 0.0 0.0 0.0 1.305660866776023E-4 10-11 0.0 0.0 0.0 0.0 1.740881155701364E-4 12-13 0.0 0.0 0.0 0.0 3.2641521669400577E-4 14-15 0.0 0.0 0.0 2.176101444626705E-5 3.916982600328069E-4 16-17 0.0 0.0 0.0 1.0880507223133525E-4 5.222643467104092E-4 18-19 0.0 0.0 0.0 1.5232710112386935E-4 5.440253611566762E-4 20-21 0.0 0.0 0.0 3.2641521669400577E-4 6.528304333880115E-4 22-23 0.0 0.0 0.0 6.093084044954774E-4 6.528304333880115E-4 24-25 0.0 0.0 0.0 0.0010445286934208185 6.528304333880115E-4 26-27 0.0 0.0 0.0 0.0013927049245610911 6.963524622805456E-4 28-29 0.0 0.0 0.0 0.0018496862279326993 7.398744911730797E-4 30-31 0.0 0.0 0.0 0.003285913181386325 7.616355056193468E-4 32-33 0.0 0.0 0.0 0.006419499261648779 8.486795634044149E-4 34-35 0.0 0.0 0.0 0.01040176490531565 9.139626067432162E-4 36-37 0.0 0.0 0.0 0.016973591268088298 9.139626067432162E-4 38-39 0.0 0.0 0.0 0.02870277805462624 9.139626067432162E-4 40-41 0.0 0.0 0.0 0.041759386722386466 9.139626067432162E-4 42-43 0.0 0.0 0.0 0.05542530379464218 9.792456500820172E-4 44-45 0.0 0.0 0.0 0.07196367477380514 0.0010227676789745514 46-47 0.0 0.0 0.0 0.09283248762777524 0.0010445286934208185 48-49 0.0 0.0 0.0 0.11563803076746311 0.0010880507223133525 50-51 0.0 0.0 0.0 0.14186005317521488 0.0012186168089909548 52-53 0.0 0.0 0.0 0.16679817573063693 0.0012621388378834888 54-55 0.0 0.0 0.0 0.19517453856856917 0.0012621388378834888 56-57 0.0 0.0 0.0 0.22968750748034872 0.001283899852329756 58-59 0.0 0.0 0.0 0.2685962013102742 0.001305660866776023 60-61 0.0 0.0 0.0 0.31314099788178285 0.001305660866776023 62-63 0.0 0.0 0.0 0.3578163605399691 0.0013927049245610911 64-65 0.0 0.0 0.0 0.40551650420618646 0.0013927049245610911 66-67 0.0 0.0 0.0 0.46050658771190334 0.0014144659390073581 68-69 0.0 0.0 0.0 0.526159568296291 0.0014362269534536254 70-71 0.0 0.0 0.0 0.5999294072691363 0.0014579879678998924 72-73 0.0 0.0 0.0 0.675026668123204 0.0015885540545774947 74-75 0.0 0.0 0.0 0.7504503441939655 0.00169735912680883 76-77 0.0 0.0 0.0 0.8378425782101739 0.001740881155701364 78-79 0.0 0.0 0.0 0.9377691565474322 0.0017844031845938983 80-81 0.0 0.0 0.0 1.0556920938317536 0.0018714472423789663 82-83 0.0 0.0 0.0 1.1792511338576577 0.002089057386841637 84-85 0.0 0.0 0.0 1.304115834750338 0.002110818401287904 86-87 0.0 0.0 0.0 1.4367492178003358 0.0022849065168580405 88-89 0.0 0.0 0.0 1.5894462561697917 0.0024589946324281766 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6135 0.0 39.81771 1 GGTATCA 3940 0.0 36.887848 1 TCAACGC 7790 0.0 30.922188 4 ATCAACG 7845 0.0 30.766394 3 CAACGCA 7820 0.0 30.743044 5 TATCAAC 8140 0.0 29.825163 2 AACGCAG 8230 0.0 29.325222 6 GTACATG 10280 0.0 28.414055 1 TACATGG 10295 0.0 27.627274 2 ACATGGG 10030 0.0 27.36695 3 TACCTGG 2145 0.0 26.254852 2 GTACCTG 2325 0.0 25.655989 1 ACGCAGA 9525 0.0 25.040672 7 CGCAGAG 9835 0.0 24.347626 8 CATGGGG 5865 0.0 24.045738 4 ACCGTAC 100 0.0016902954 23.661945 8 GAGTACA 7635 0.0 21.081963 1 CATGGGA 4035 0.0 20.994204 4 AGTACAT 7145 0.0 20.86403 2 GCAGAGT 11200 0.0 20.78871 9 >>END_MODULE