FastQCFastQC Report
Thu 26 May 2016
SRR936297_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936297_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2297687
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47090.20494523405494308No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35470.15437263648181845No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23390.10179802557963726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63400.039.6875951
GGTATCA40850.039.0068741
ATCAACG78650.031.7818683
CAACGCA79300.031.5811715
TCAACGC79100.031.5410944
TATCAAC80300.031.2537522
AACGCAG81650.030.7884066
GTACATG101450.028.4593521
TACATGG101450.028.011532
ACATGGG100700.026.6125493
ACGCAGA97400.025.56637
TACCTGG21000.025.076432
GTACCTG22400.025.0566141
CGCAGAG99800.024.8088978
CATGGGG61450.024.0824244
TATACCG2103.5030098E-822.5864625
AGTACAT70100.021.3183982
GAGTACA76100.021.0011461
GCAGAGT117500.020.9506249
GAGTACT55750.020.03611812-13
Produced by FastQC (version 0.11.5)