##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936297_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2297687 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49206876306477 34.0 31.0 34.0 31.0 34.0 2 32.620924434006895 34.0 31.0 34.0 31.0 34.0 3 32.70709805121411 34.0 31.0 34.0 31.0 34.0 4 36.1904811229728 37.0 35.0 37.0 35.0 37.0 5 36.151403998891055 37.0 35.0 37.0 35.0 37.0 6 36.18726179849562 37.0 37.0 37.0 35.0 37.0 7 36.135939751584964 37.0 36.0 37.0 35.0 37.0 8 36.11308328767147 37.0 36.0 37.0 35.0 37.0 9 37.88412607983594 39.0 38.0 39.0 35.0 39.0 10-11 37.81964427704905 39.0 38.0 39.0 35.0 39.0 12-13 37.76477975459669 39.0 38.0 39.0 35.0 39.0 14-15 39.12392418984831 40.5 38.5 41.0 36.0 41.0 16-17 39.047481445471035 40.0 38.0 41.0 36.0 41.0 18-19 39.06369318362336 40.0 38.5 41.0 36.0 41.0 20-21 39.014379025515666 40.0 38.5 41.0 35.0 41.0 22-23 38.89566594579679 40.0 38.0 41.0 35.0 41.0 24-25 39.04867373145254 40.0 39.0 41.0 35.0 41.0 26-27 38.97429893627809 40.0 38.5 41.0 35.0 41.0 28-29 38.88548222625623 40.0 38.0 41.0 35.0 41.0 30-31 38.77741811656679 40.0 38.0 41.0 35.0 41.0 32-33 38.63256396541391 40.0 38.0 41.0 34.5 41.0 34-35 38.49691102399935 40.0 38.0 41.0 34.0 41.0 36-37 38.35451869641078 40.0 38.0 41.0 34.0 41.0 38-39 38.22500170823963 40.0 38.0 41.0 33.5 41.0 40-41 38.0845158631267 40.0 38.0 41.0 33.0 41.0 42-43 38.10742564152558 40.0 38.0 41.0 33.0 41.0 44-45 37.98149530375547 40.0 38.0 41.0 33.0 41.0 46-47 37.78059196052378 40.0 37.0 41.0 33.0 41.0 48-49 37.55182516156465 40.0 37.0 41.0 33.0 41.0 50-51 37.34687796901841 40.0 37.0 41.0 32.0 41.0 52-53 37.127341104336665 40.0 36.0 41.0 32.0 41.0 54-55 36.921780686403324 39.5 36.0 41.0 31.0 41.0 56-57 36.66067005645242 39.0 35.0 41.0 31.0 41.0 58-59 36.38728947850599 39.0 35.0 41.0 31.0 41.0 60-61 36.10291001341784 38.5 35.0 40.5 30.0 41.0 62-63 35.791081857537606 38.0 35.0 40.0 30.0 41.0 64-65 35.41219126016729 37.0 35.0 40.0 29.0 41.0 66-67 35.05546969626411 37.0 34.0 39.5 29.0 41.0 68-69 34.6940357846826 36.0 34.0 39.0 29.0 41.0 70-71 34.31623236759402 36.0 34.0 39.0 28.5 40.5 72-73 33.91215143751085 35.0 34.0 38.0 28.0 40.0 74-75 33.4860999344123 35.0 33.5 37.0 27.0 39.0 76-77 32.77156745022277 35.0 32.5 36.0 26.0 39.0 78-79 32.830664489984926 35.0 33.0 36.0 26.5 39.0 80-81 32.666287879941876 35.0 33.0 36.0 26.0 37.5 82-83 32.39622563908836 35.0 33.0 35.5 26.0 37.0 84-85 32.11370543507449 35.0 33.0 35.0 26.0 37.0 86-87 31.72120789298107 35.0 32.5 35.0 25.0 36.0 88-89 31.234242087803953 35.0 32.0 35.0 23.5 36.0 90-91 31.021146701008448 34.0 32.0 35.0 23.0 35.5 92-93 30.7454559737684 34.0 31.5 35.0 20.0 35.0 94-95 30.588981223291075 34.0 31.0 35.0 20.0 35.0 96-97 30.33846363756247 34.0 31.0 35.0 18.5 35.0 98-99 29.881796998459755 34.0 31.0 35.0 2.0 35.0 100-101 26.48082767583226 31.0 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13869559148905353 1101 2 0.16013282385029015 1101 3 0.2432047095092642 1101 4 0.15332936546642628 1101 5 0.17395031643190606 1101 6 0.21032450442870498 1101 7 0.20687427896005772 1101 8 0.14898905507268978 1101 9 0.2048323928553728 1101 10-11 0.17201802631051066 1101 12-13 0.30116657953899306 1101 14-15 0.39049360742638584 1101 16-17 0.3988767478077193 1101 18-19 0.3721951304428046 1101 20-21 0.2637647809539132 1101 22-23 0.2095928296983871 1101 24-25 0.19707042108975514 1101 26-27 0.27413792980635066 1101 28-29 0.24766682185100564 1101 30-31 0.2436953419727459 1101 32-33 0.3519719839925912 1101 34-35 0.32772095445535143 1101 36-37 0.3067315170506717 1101 38-39 0.37728013683662454 1101 40-41 0.42097681864134273 1101 42-43 0.40569092329795353 1101 44-45 0.39289464502207494 1101 46-47 0.27744764359585616 1101 48-49 0.34107378441961345 1101 50-51 0.3757465768680319 1101 52-53 0.3562195003167261 1101 54-55 0.3092769180365522 1101 56-57 0.2811941168660965 1101 58-59 0.2115111129880134 1101 60-61 0.26367268363909346 1101 62-63 0.23335443591923166 1101 64-65 0.3959800242111129 1101 66-67 0.31968966360143725 1101 68-69 0.44621004944051634 1101 70-71 0.3780656058221794 1101 72-73 0.440872486134424 1101 74-75 0.3715916492418927 1101 76-77 0.3834738551779182 1101 78-79 0.2601480473389586 1101 80-81 0.3713967712811339 1101 82-83 0.39115237513694723 1101 84-85 0.44927312407873643 1101 86-87 0.5337096653294786 1101 88-89 0.5893115323517044 1101 90-91 0.7095162015259078 1101 92-93 0.8285586203118278 1101 94-95 0.6814600194013174 1101 96-97 0.561760792185094 1101 98-99 0.5074143123635473 1101 100-101 0.634914798752817 1102 1 0.12015229800204708 1102 2 0.21160468390318954 1102 3 0.16647610915677546 1102 4 0.16031033151089957 1102 5 0.11171389599555681 1102 6 0.1715647245962728 1102 7 0.16468011698107432 1102 8 0.21430081205125617 1102 9 0.09075523334987423 1102 10-11 0.20209616411443676 1102 12-13 0.24332468567428833 1102 14-15 0.282294054720559 1102 16-17 0.25992130829203575 1102 18-19 0.2917044936159172 1102 20-21 0.26278950820280755 1102 22-23 0.2665759389223794 1102 24-25 0.2397925616470431 1102 26-27 0.2967932283764725 1102 28-29 0.27250841567057904 1102 30-31 0.20724654001008957 1102 32-33 0.23377323813081574 1102 34-35 0.2443877051682719 1102 36-37 0.10196596828324545 1102 38-39 0.13973049701684914 1102 40-41 0.10504984307106469 1102 42-43 0.14747460336411677 1102 44-45 0.017978670266209917 1102 46-47 0.1185128542451892 1102 48-49 0.12269400292306898 1102 50-51 -0.02828688698913595 1102 52-53 -0.05449286734194203 1102 54-55 -0.044867739016901 1102 56-57 0.11739569676760908 1102 58-59 0.036202684241168015 1102 60-61 0.04331159571074039 1102 62-63 0.015003146103367726 1102 64-65 0.01212207414972255 1102 66-67 0.012497531339519696 1102 68-69 0.013928723089755124 1102 70-71 0.07641006267744643 1102 72-73 0.012525533105353759 1102 74-75 0.09502517411664257 1102 76-77 -0.14415135063034512 1102 78-79 -0.09126090409155552 1102 80-81 -0.014321962881396644 1102 82-83 0.06878338013686403 1102 84-85 0.1851901653815844 1102 86-87 0.08343596869780256 1102 88-89 0.11133875325299769 1102 90-91 0.33980197560453007 1102 92-93 0.31405131206966175 1102 94-95 0.199775883720406 1102 96-97 -0.024232899302955246 1102 98-99 0.01824594165904614 1102 100-101 0.5598237136232029 1103 1 -0.001346751211741548 1103 2 0.016384266793259883 1103 3 0.049407767811530334 1103 4 0.0031390197986951307 1103 5 0.06917963998024845 1103 6 0.08114537319089266 1103 7 0.16073448910167798 1103 8 0.09152971536338583 1103 9 0.1424466616578428 1103 10-11 0.16394712439134196 1103 12-13 0.15932590009092706 1103 14-15 0.16657120951307292 1103 16-17 0.2036928616123248 1103 18-19 0.1845283016753072 1103 20-21 0.1974812616958488 1103 22-23 0.25134435369177055 1103 24-25 0.14601676571611932 1103 26-27 0.16449514108267493 1103 28-29 0.2385466854898297 1103 30-31 0.287850133525815 1103 32-33 0.13554552127823882 1103 34-35 0.18880644053677997 1103 36-37 0.18886358501512035 1103 38-39 0.25501878118249977 1103 40-41 0.35760472847398006 1103 42-43 0.3627604842012033 1103 44-45 0.16462450645042992 1103 46-47 0.12041050285794341 1103 48-49 0.009462403959147991 1103 50-51 0.08552715129441424 1103 52-53 0.08206082816810323 1103 54-55 0.06385872246436008 1103 56-57 0.12622277923954783 1103 58-59 0.1434395724898181 1103 60-61 0.05911740480110694 1103 62-63 0.05776780285163596 1103 64-65 0.04763885600448958 1103 66-67 0.01786504687164836 1103 68-69 0.07792163768477423 1103 70-71 -0.08970432889540092 1103 72-73 0.03969185177985679 1103 74-75 0.11751358541346235 1103 76-77 0.09368989137060879 1103 78-79 -0.002160563162227902 1103 80-81 0.14953354508171657 1103 82-83 0.24070291375379327 1103 84-85 0.2308353830765597 1103 86-87 0.15677145521733493 1103 88-89 0.15527993742692914 1103 90-91 0.24388157678932032 1103 92-93 0.38735111651719123 1103 94-95 0.2417723336607871 1103 96-97 0.2707728613284637 1103 98-99 0.24819249457469894 1103 100-101 0.2735974593677213 1104 1 0.022079757018047985 1104 2 0.11520248816415801 1104 3 0.033807046822033726 1104 4 0.037645938698680936 1104 5 0.033423985354559704 1104 6 0.03268340877703224 1104 7 0.03378581157496541 1104 8 0.03728457893477355 1104 9 -0.020696988153716234 1104 10-11 0.05180811354824044 1104 12-13 0.12197824867465812 1104 14-15 0.1274578118783225 1104 16-17 0.1446724411104583 1104 18-19 0.27000595721636245 1104 20-21 0.15728440218143902 1104 22-23 0.17592085711873295 1104 24-25 0.17226140491059994 1104 26-27 0.30192740705773957 1104 28-29 0.2702743198419242 1104 30-31 0.11648728821648291 1104 32-33 0.14869185502404747 1104 34-35 0.16921866458596924 1104 36-37 0.059074881270611 1104 38-39 0.1944713433508909 1104 40-41 0.21067627933594935 1104 42-43 0.24115001064218688 1104 44-45 0.25892736159143936 1104 46-47 0.25800239229116073 1104 48-49 0.11839270077402375 1104 50-51 0.16564258871919435 1104 52-53 0.13395257803673388 1104 54-55 0.13936909375134832 1104 56-57 0.13592732045629674 1104 58-59 0.08161161550057017 1104 60-61 -0.0161040904045322 1104 62-63 0.08451462027066015 1104 64-65 0.032543138433482 1104 66-67 0.06445249509997097 1104 68-69 0.23094756166445762 1104 70-71 0.2633968865030383 1104 72-73 0.17845902189608154 1104 74-75 0.23487991136174458 1104 76-77 0.1573330354724547 1104 78-79 0.04759322118506759 1104 80-81 0.05838784417934306 1104 82-83 0.13771611871030842 1104 84-85 0.253417158315429 1104 86-87 0.09672972710270145 1104 88-89 0.23212168136265277 1104 90-91 0.4180456018996459 1104 92-93 0.40353877154970874 1104 94-95 0.2719870766528949 1104 96-97 0.24879567759479926 1104 98-99 0.11387217973508612 1104 100-101 0.3960802115586546 1105 1 -0.06491450822865374 1105 2 0.04177981888224025 1105 3 0.05403096573121502 1105 4 0.015343411084963066 1105 5 0.007587503788471395 1105 6 0.06258295712759576 1105 7 0.05504675516027646 1105 8 0.039107180008770115 1105 9 0.09901746873168094 1105 10-11 0.062114985312810234 1105 12-13 0.0998250398481133 1105 14-15 0.08556693906327695 1105 16-17 0.11351667722290415 1105 18-19 0.10970984552317731 1105 20-21 0.022783965851431276 1105 22-23 -0.09364389343455315 1105 24-25 0.06477239522926936 1105 26-27 0.012061456574123497 1105 28-29 -0.09219065293417827 1105 30-31 -0.007294224886642553 1105 32-33 0.15231551423251943 1105 34-35 0.18199932691395304 1105 36-37 0.2946599191177981 1105 38-39 0.12366266471501319 1105 40-41 0.21494702461679083 1105 42-43 0.30908413164922877 1105 44-45 0.29090266905135564 1105 46-47 0.2700939085219929 1105 48-49 0.2816951353769568 1105 50-51 0.24499104815965467 1105 52-53 0.24356465010732364 1105 54-55 0.30751527023293335 1105 56-57 0.28689203380526607 1105 58-59 0.24677962307274726 1105 60-61 0.3723496463038103 1105 62-63 0.3717174530874203 1105 64-65 0.3566119819172968 1105 66-67 0.32532586638535577 1105 68-69 0.20366200395992706 1105 70-71 0.18536357222779287 1105 72-73 0.20541835794497132 1105 74-75 0.33771735586302754 1105 76-77 0.18596394405106054 1105 78-79 0.09513164968576149 1105 80-81 0.1578449849001089 1105 82-83 0.15109667781456437 1105 84-85 0.18826469813453173 1105 86-87 0.2891689304197165 1105 88-89 0.26579534754694123 1105 90-91 0.30608101803628784 1105 92-93 0.3181773243383468 1105 94-95 0.21891587416020641 1105 96-97 0.21968672092961938 1105 98-99 0.10068687489223294 1105 100-101 0.32094516354411695 1106 1 0.03962577431808967 1106 2 0.0867976000904207 1106 3 0.013131122331934364 1106 4 0.050962303514673124 1106 5 0.0752151977301665 1106 6 0.08980376939367574 1106 7 0.053931024296360874 1106 8 0.05380081042670071 1106 9 -0.07333504005384839 1106 10-11 0.12016254167177465 1106 12-13 0.09973737058952992 1106 14-15 0.1970964644497073 1106 16-17 0.11397560007088714 1106 18-19 0.1220612510551149 1106 20-21 0.1987293819422078 1106 22-23 0.031144157103220493 1106 24-25 0.1274556962165505 1106 26-27 -0.045528161984698556 1106 28-29 0.1125448660962931 1106 30-31 0.12775277065174606 1106 32-33 0.15446083899842478 1106 34-35 0.21897835152394407 1106 36-37 0.18264717303642186 1106 38-39 0.21376389698798448 1106 40-41 0.029594839364754932 1106 42-43 0.08738663590354179 1106 44-45 -0.04130154406396258 1106 46-47 0.06942882610413648 1106 48-49 0.08155858585509179 1106 50-51 0.1616615820512095 1106 52-53 0.08385490211759361 1106 54-55 0.11248816390511962 1106 56-57 0.20527009341861202 1106 58-59 0.20880865527855264 1106 60-61 0.11675632571626693 1106 62-63 -0.020106671653785213 1106 64-65 0.08770530184814618 1106 66-67 0.1803535287179585 1106 68-69 0.1859094716208034 1106 70-71 0.17380819480780474 1106 72-73 0.07730139292554838 1106 74-75 0.21750879277639967 1106 76-77 0.2493680811644694 1106 78-79 0.09570418138569181 1106 80-81 0.233277869649946 1106 82-83 0.2647567522491556 1106 84-85 0.3439567642964718 1106 86-87 0.30968528812430307 1106 88-89 0.39340391842638667 1106 90-91 0.46729329234540984 1106 92-93 0.5474844241692232 1106 94-95 0.6425462089057312 1106 96-97 0.6374170568572026 1106 98-99 0.47411785784302296 1106 100-101 0.9186145184428511 1107 1 0.08047434357876426 1107 2 0.10350601614297261 1107 3 0.09397075271994737 1107 4 0.07680188193248938 1107 5 0.07932084273219431 1107 6 0.08882153280458027 1107 7 0.0614095346228396 1107 8 0.07920903626835951 1107 9 0.07744607190033292 1107 10-11 0.08626758381960542 1107 12-13 0.07483585409939053 1107 14-15 0.13294965905728162 1107 16-17 0.20159993875022053 1107 18-19 0.1284535844127035 1107 20-21 0.12628033871340705 1107 22-23 0.09956810912434122 1107 24-25 0.15616143060730536 1107 26-27 0.26064901478670066 1107 28-29 0.26314148387633196 1107 30-31 0.13196755305256858 1107 32-33 0.0853222968661882 1107 34-35 0.05093534099479058 1107 36-37 0.07426594051140256 1107 38-39 0.08744515341929571 1107 40-41 0.06596617676562033 1107 42-43 -0.0703117083262157 1107 44-45 -0.08338440533473346 1107 46-47 -0.04589562379140233 1107 48-49 -0.13859753259535523 1107 50-51 -0.10228721691770204 1107 52-53 0.00964147094171608 1107 54-55 0.08070059725334744 1107 56-57 -0.0502933154100802 1107 58-59 -0.053708364958637844 1107 60-61 0.11447223587020261 1107 62-63 0.04829909080086736 1107 64-65 0.1333767047314538 1107 66-67 9.576302053417862E-4 1107 68-69 0.12564483941383742 1107 70-71 0.26971658175705215 1107 72-73 0.08561402479990221 1107 74-75 0.06663673298989181 1107 76-77 -0.07601987630312834 1107 78-79 0.06752334591292453 1107 80-81 0.26269559564232026 1107 82-83 0.3881495741265226 1107 84-85 0.22112002345102155 1107 86-87 0.19735691967644797 1107 88-89 0.3878483051261732 1107 90-91 0.16434346436673763 1107 92-93 0.4256039348179179 1107 94-95 0.40547899066211457 1107 96-97 0.044260491463116836 1107 98-99 0.24051705126568024 1107 100-101 0.3862708707591622 1108 1 -0.11765824173400574 1108 2 -0.073775597258809 1108 3 -0.0495571424551855 1108 4 -0.0016868186570349053 1108 5 -0.04633348692652106 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66-67 -0.6716168690618787 2316 68-69 -0.6289556093619595 2316 70-71 -0.6389985284083011 2316 72-73 -0.7367725185500191 2316 74-75 -0.7031660374968851 2316 76-77 -0.5420350452564549 2316 78-79 -0.683096991414132 2316 80-81 -0.7379184488875019 2316 82-83 -0.8315765235939878 2316 84-85 -0.5453193297156602 2316 86-87 -0.5978336444397065 2316 88-89 -0.5410001957804838 2316 90-91 -0.6321599759717493 2316 92-93 -0.760655245628044 2316 94-95 -0.5708702761442019 2316 96-97 -0.5180143547359535 2316 98-99 -0.6675077556034061 2316 100-101 -0.5766412867594894 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 18.0 5 35.0 6 68.0 7 190.0 8 349.0 9 709.0 10 1186.0 11 1793.0 12 2708.0 13 4112.0 14 5043.0 15 6911.0 16 8820.0 17 12139.0 18 11478.0 19 7661.0 20 7058.0 21 7434.0 22 8140.0 23 9170.0 24 11025.0 25 13425.0 26 16611.0 27 20879.0 28 26509.0 29 32689.0 30 40520.0 31 50471.0 32 63460.0 33 82403.0 34 115822.0 35 172759.0 36 301309.0 37 553304.0 38 593916.0 39 107492.0 40 65.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.00202924734562 17.99065237029182 13.07642625540587 23.930892126956696 2 16.136089886153552 19.147211604302075 39.55111772200885 25.165580787535525 3 20.220204057384667 23.243200662231192 28.20484252206676 28.331752758317386 4 13.088205660736211 14.945638809811781 35.33510003755951 36.6310554918925 5 14.237709487845821 37.245543017826186 33.50599972929298 15.010747765035012 6 34.99323450060866 34.25971422565389 16.344219208273365 14.402832065464095 7 30.84697785207472 31.256737754097923 19.39006487828847 18.506219515538888 8 27.760264996929525 34.96577210037747 18.518666815802153 18.75529608689086 9 27.178506036723014 15.762286159951291 18.907231489754697 38.151976313571 10-11 26.424780490394976 25.760740678036548 26.1951138943068 21.619364937261675 12-13 26.473254601914537 22.57639754796676 27.252582490637316 23.69776535948139 14-15 23.206794485062588 24.18488680137895 24.89575386029516 27.712564853263306 16-17 23.454216941142985 27.516665190539058 25.60440897192536 23.4247088963926 18-19 23.382688764831762 26.298403568458195 27.412175809847035 22.90673185686301 20-21 23.99009264103102 25.437157209470744 27.53422356231443 23.038526587183803 22-23 23.645108804102566 25.2448062386285 27.466129488186912 23.643955469082027 24-25 23.465821970542702 25.413414947831047 27.767295967019944 23.353467114606303 26-27 23.23705955458915 25.794584553702364 27.541775084472857 23.426580807235624 28-29 23.164920049189785 25.94297649557816 27.623772567533134 23.26833088769892 30-31 23.314005677521564 25.606797357743705 27.52877659094099 23.550420373793745 32-33 23.25269296890301 25.634213217853024 27.512632472308663 23.600461340935304 34-35 23.533584837337727 25.77192115053507 27.437344514641953 23.257149497485248 36-37 23.656327204049465 25.53793806854768 27.42370528951852 23.38202943788434 38-39 23.319955372463017 25.849480204962866 27.340372448871896 23.49019197370222 40-41 23.508032049859207 25.666936505242266 27.43313878842479 23.391892656473733 42-43 23.41130340430477 25.601249308514873 27.30170426329142 23.685743023888932 44-45 23.40649679338613 25.775256300609982 27.273779756658595 23.544467149345294 46-47 23.596711446278068 26.091681221400627 26.87414371915621 23.437463613165097 48-49 23.564759527479914 26.027802135642435 26.913499799659736 23.49393853721791 50-51 23.606892232267253 26.24168513929881 26.68163337965818 23.469789248775758 52-53 23.782439675571144 26.100858122984643 26.653977591871563 23.46272460957265 54-55 23.696742075520273 26.106461119710385 26.57943934182148 23.617357462947865 56-57 23.505270357581747 26.438203623801993 26.582331370210277 23.474194648405987 58-59 23.664906540683283 25.980003547090895 26.715752891803934 23.639337020421884 60-61 23.443365011262564 26.199085894693138 26.907133420509115 23.45041567353519 62-63 23.400054054359885 26.135130677067853 26.95090758662951 23.513907681942754 64-65 23.382895979809064 26.151794572271104 26.755189889559595 23.71011955836024 66-67 23.364648883855807 26.308718289305723 26.60936846489535 23.71726436194312 68-69 23.472496471451507 26.069194803295666 26.888018254879796 23.57029047037303 70-71 23.541149932344887 26.07428875088513 26.68316299506938 23.7013983217006 72-73 23.54800493896152 26.209551589472547 26.514907194266833 23.727536277299098 74-75 23.32412987495686 26.48815961442964 26.496189428760314 23.691521081853185 76-77 23.497531435683342 26.38999151597955 26.4286503909823 23.68382665735481 78-79 23.596898819521144 26.374520478116807 26.535962235132914 23.492618467229132 80-81 23.46894719868248 26.432256247793944 26.43673908700669 23.662057466516888 82-83 23.693980076485612 26.468552940413552 26.37245630061884 23.46501068248199 84-85 23.537911416424972 26.469226242910217 26.281209850666237 23.71165248999857 86-87 23.479402360707883 26.271813670753698 26.547385736618207 23.701398231920212 88-89 23.561964411003977 26.409303007781332 26.267424258179485 23.76130832303521 90-91 23.318041845439687 26.59238790402121 26.385326284142245 23.704243966396856 92-93 23.414826959727684 26.4325443699162 26.353076189054388 23.799552481301735 94-95 23.42733363378727 26.280898898410516 26.477209495243915 23.814557972558298 96-97 23.48617264821859 26.175716611101922 26.4922833192608 23.845827421418686 98-99 23.25942335856965 26.369517956663756 26.311261895487277 24.059796789279318 100-101 23.23324511364324 26.39163623370253 26.357057771057935 24.01806088159629 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 97.0 1 72.0 2 107.5 3 195.0 4 168.0 5 146.5 6 298.0 7 610.0 8 1002.0 9 1178.5 10 1062.5 11 969.5 12 1042.5 13 1147.5 14 1240.5 15 1387.5 16 1505.0 17 1635.0 18 1828.5 19 2015.5 20 2246.5 21 2555.0 22 3140.0 23 4010.0 24 4872.5 25 6154.5 26 8128.0 27 10390.5 28 12591.5 29 15094.0 30 17994.0 31 22005.0 32 27005.0 33 30589.0 34 34017.5 35 38004.5 36 42985.5 37 48295.0 38 52436.5 39 56218.5 40 59377.0 41 63387.5 42 67172.0 43 70621.0 44 75025.5 45 78897.5 46 81981.5 47 84471.0 48 86917.5 49 89053.5 50 89970.5 51 90197.5 52 90100.5 53 91404.0 54 92703.5 55 88578.0 56 81092.5 57 74340.0 58 69503.0 59 63904.0 60 54829.5 61 45644.0 62 37782.0 63 29249.5 64 21564.5 65 16328.0 66 11965.5 67 9231.5 68 7104.0 69 5164.0 70 3939.0 71 2594.5 72 1925.5 73 1639.0 74 1280.5 75 996.5 76 617.5 77 329.5 78 197.5 79 96.5 80 48.0 81 27.5 82 17.5 83 10.0 84 6.0 85 3.5 86 1.5 87 2.5 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26970601304703384 2 0.020455353579491027 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.6993724558653985E-4 12-13 8.704405778506821E-5 14-15 0.0 16-17 3.6993724558653985E-4 18-19 0.0 20-21 4.3522028892534105E-5 22-23 1.0880507223133527E-4 24-25 0.0024372336179819096 26-27 0.0024154726035356426 28-29 0.0024154726035356426 30-31 0.0012838998523297558 32-33 0.0016103150690237617 34-35 0.0018714472423789663 36-37 6.528304333880115E-5 38-39 1.0880507223133527E-4 40-41 7.398744911730797E-4 42-43 0.004178114773683274 44-45 0.004004026658113137 46-47 0.014122898375627316 48-49 0.015036860982370531 50-51 0.00626717216052491 52-53 0.007507549983962132 54-55 5.440253611566762E-4 56-57 0.0031553470947087224 58-59 0.0012838998523297558 60-61 0.00132742188122229 62-63 0.0 64-65 9.792456500820172E-4 66-67 0.0 68-69 0.0 70-71 1.7408811557013642E-4 72-73 0.0016538370979162957 74-75 0.0 76-77 0.1981340365332615 78-79 0.10584557426664293 80-81 0.0015667930401312275 82-83 0.0 84-85 6.528304333880115E-4 86-87 0.12292797060696257 88-89 0.5960124246688082 90-91 0.3179719430888542 92-93 0.2973860234226855 94-95 0.00228490651685804 96-97 0.002915975935799785 98-99 0.003133586080262455 100-101 6.093084044954774E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2297687.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.2007385913783 #Duplication Level Percentage of deduplicated Percentage of total 1 68.68018415793514 31.730752346884827 2 15.528573114687932 14.348630943376046 3 6.2137914120304 8.612452580657038 4 3.019875729030691 5.580819565415797 5 1.7382260378138588 4.015366339288266 6 1.083556412842032 3.0036663947235787 7 0.7104136876146184 2.297514595125405 8 0.5130210562131652 1.896156136798179 9 0.3980540558468483 1.655135224147632 >10 1.914481033053212 15.816298525744136 >50 0.13033450465024307 4.138994259154736 >100 0.06590911016488929 5.306644901468081 >500 0.0030144742036736804 0.9824258776264682 >1k 5.652139131887488E-4 0.6151423095898343 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4709 0.20494523405494308 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3547 0.15437263648181845 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2339 0.10179802557963726 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.305660866776023E-4 2 0.0 0.0 0.0 0.0 1.305660866776023E-4 3 0.0 0.0 0.0 0.0 1.305660866776023E-4 4 0.0 0.0 0.0 0.0 1.305660866776023E-4 5 0.0 0.0 0.0 0.0 1.305660866776023E-4 6 0.0 0.0 0.0 0.0 1.305660866776023E-4 7 0.0 0.0 0.0 0.0 1.305660866776023E-4 8 0.0 0.0 0.0 0.0 1.305660866776023E-4 9 0.0 0.0 0.0 0.0 1.305660866776023E-4 10-11 0.0 0.0 0.0 0.0 1.305660866776023E-4 12-13 0.0 0.0 0.0 0.0 5.005033322641422E-4 14-15 0.0 0.0 0.0 2.176101444626705E-5 5.440253611566762E-4 16-17 0.0 0.0 0.0 1.0880507223133525E-4 5.657863756029433E-4 18-19 0.0 0.0 0.0 1.5232710112386935E-4 6.528304333880115E-4 20-21 0.0 0.0 0.0 3.2641521669400577E-4 7.398744911730797E-4 22-23 0.0 0.0 0.0 6.093084044954774E-4 7.833965200656138E-4 24-25 0.0 0.0 0.0 0.0010445286934208185 7.833965200656138E-4 26-27 4.35220288925341E-5 0.0 0.0 0.0013927049245610911 7.833965200656138E-4 28-29 4.35220288925341E-5 0.0 0.0 0.0018496862279326993 7.833965200656138E-4 30-31 4.35220288925341E-5 0.0 0.0 0.0033511962247251255 8.051575345118809E-4 32-33 4.35220288925341E-5 0.0 0.0 0.006593587377218916 8.269185489581479E-4 34-35 4.35220288925341E-5 0.0 0.0 0.010597614035332055 9.139626067432162E-4 36-37 4.35220288925341E-5 0.0 0.0 0.01734352851367484 9.139626067432162E-4 38-39 4.35220288925341E-5 0.0 0.0 0.029225042401336648 9.357236211894832E-4 40-41 4.35220288925341E-5 0.0 0.0 0.04245573918466702 9.574846356357502E-4 42-43 4.35220288925341E-5 0.0 0.0 0.05620870031470779 9.574846356357502E-4 44-45 4.35220288925341E-5 0.0 0.0 0.07259474419274688 9.792456500820172E-4 46-47 4.35220288925341E-5 0.0 0.0 0.09365940617673338 0.0010445286934208185 48-49 4.35220288925341E-5 0.0 0.0 0.1168348865620078 0.0012186168089909548 50-51 4.35220288925341E-5 0.0 0.0 0.1431874750564372 0.001305660866776023 52-53 4.35220288925341E-5 0.0 0.0 0.16799503152518164 0.001349182895668557 54-55 4.35220288925341E-5 0.0 0.0 0.19632787233422133 0.0013927049245610911 56-57 4.35220288925341E-5 0.0 0.0 0.23125430052047993 0.0014362269534536254 58-59 4.35220288925341E-5 0.0 0.0 0.2706852586971158 0.0014362269534536254 60-61 4.35220288925341E-5 0.0 0.0 0.3153606213553021 0.0014362269534536254 62-63 4.35220288925341E-5 0.0 0.0 0.36023183314350476 0.0014797489823461594 64-65 4.35220288925341E-5 0.0 0.0 0.40795373782416844 0.0014797489823461594 66-67 4.35220288925341E-5 0.0 0.0 0.4632919975610255 0.0015015099967924264 68-69 4.35220288925341E-5 0.0 0.0 0.5292931543765536 0.0015232710112386934 70-71 4.35220288925341E-5 0.0 0.0 0.6030629933493987 0.0015232710112386934 72-73 4.35220288925341E-5 0.0 0.0 0.6788348456513006 0.0015667930401312277 74-75 4.35220288925341E-5 0.0 0.0 0.7544108488231861 0.00169735912680883 76-77 4.35220288925341E-5 0.0 0.0 0.8416289947238245 0.00169735912680883 78-79 4.35220288925341E-5 0.0 0.0 0.9412291578443888 0.001719120141255097 80-81 4.35220288925341E-5 0.0 0.0 1.0593697052731725 0.0017844031845938983 82-83 4.35220288925341E-5 0.0 0.0 1.1826893741401678 0.0018714472423789663 84-85 4.35220288925341E-5 0.0 0.0 1.3073799869172782 0.0018714472423789663 86-87 4.35220288925341E-5 0.0 0.0 1.4394040615627803 0.0019802523146103014 88-89 4.35220288925341E-5 0.0 0.0 1.5922751880478063 0.0020020133290565684 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6340 0.0 39.687595 1 GGTATCA 4085 0.0 39.006874 1 ATCAACG 7865 0.0 31.781868 3 CAACGCA 7930 0.0 31.581171 5 TCAACGC 7910 0.0 31.541094 4 TATCAAC 8030 0.0 31.253752 2 AACGCAG 8165 0.0 30.788406 6 GTACATG 10145 0.0 28.459352 1 TACATGG 10145 0.0 28.01153 2 ACATGGG 10070 0.0 26.612549 3 ACGCAGA 9740 0.0 25.5663 7 TACCTGG 2100 0.0 25.07643 2 GTACCTG 2240 0.0 25.056614 1 CGCAGAG 9980 0.0 24.808897 8 CATGGGG 6145 0.0 24.082424 4 TATACCG 210 3.5030098E-8 22.586462 5 AGTACAT 7010 0.0 21.318398 2 GAGTACA 7610 0.0 21.001146 1 GCAGAGT 11750 0.0 20.950624 9 GAGTACT 5575 0.0 20.036118 12-13 >>END_MODULE