FastQCFastQC Report
Thu 26 May 2016
SRR936296_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936296_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2384629
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29270.12274446045904834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53700.036.325491
GGTATCA32650.033.7879031
TCAACGC61000.031.1154124
ATCAACG61650.030.7873483
CAACGCA63050.029.9535855
AACGCAG66000.028.826276
TATCAAC66900.028.5835552
GTACATG85000.026.569781
TACATGG85000.026.2278672
ACATGGG82900.025.521963
ACGCAGA76950.024.7242897
CGCAGAG77850.024.37928
GTGTAGC17250.022.5067751
GTACCTG22400.022.4050751
TAGCGCG1503.1187956E-522.088584
CATGGGG51800.021.1991864
TACCTGG22200.021.1078132
TAGCCCT18850.020.590344
GAGTACA67700.020.561141
AGTACAT56800.020.4163822
Produced by FastQC (version 0.11.5)