FastQCFastQC Report
Thu 26 May 2016
SRR936296_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936296_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2384629
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34130.14312498925409362No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25000.1048381110856238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53850.036.4884761
GGTATCA33200.034.2492641
ATCAACG63050.030.3956153
TCAACGC63000.030.3444424
CAACGCA64350.029.6341295
TATCAAC67650.028.6158852
AACGCAG67000.028.4620326
GTACATG79950.027.3140181
TACATGG77150.027.2447382
ACATGGG77350.026.2481123
ACGCAGA80300.023.5706757
CGCAGAG80800.023.248698
CATGGGG47750.022.0543484
GTACCTG22250.020.9549881
GTATAGC10300.020.7857971
GCAGAGT97900.018.897169
TACCTGG23150.018.8560922
GGACCGT3105.682523E-918.3626026
GAGTACT45950.017.70489112-13
GTATAGG6800.017.4913161
Produced by FastQC (version 0.11.5)