##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936296_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2384629 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49717629031602 34.0 31.0 34.0 31.0 34.0 2 32.636618107051454 34.0 31.0 34.0 31.0 34.0 3 32.72614566039413 34.0 31.0 34.0 31.0 34.0 4 36.202372360648134 37.0 35.0 37.0 35.0 37.0 5 36.162399685653405 37.0 35.0 37.0 35.0 37.0 6 36.193968957015954 37.0 37.0 37.0 35.0 37.0 7 36.14517688076426 37.0 37.0 37.0 35.0 37.0 8 36.12301536213809 37.0 36.0 37.0 35.0 37.0 9 37.9016052392217 39.0 38.0 39.0 35.0 39.0 10-11 37.837153703993366 39.0 38.0 39.0 35.0 39.0 12-13 37.78336462401489 39.0 38.0 39.0 35.0 39.0 14-15 39.15290240117016 41.0 39.0 41.0 36.0 41.0 16-17 39.077919668007056 40.0 38.0 41.0 36.0 41.0 18-19 39.08853788157403 40.0 38.5 41.0 36.0 41.0 20-21 39.03639182447249 40.0 39.0 41.0 35.5 41.0 22-23 38.91839422400717 40.0 38.0 41.0 35.0 41.0 24-25 39.080604991384405 40.0 39.0 41.0 35.5 41.0 26-27 39.01105308205176 40.0 39.0 41.0 35.0 41.0 28-29 38.93066908940553 40.0 38.0 41.0 35.0 41.0 30-31 38.83449249338157 40.0 38.0 41.0 35.0 41.0 32-33 38.70623208054586 40.0 38.0 41.0 35.0 41.0 34-35 38.57992773718679 40.0 38.0 41.0 34.0 41.0 36-37 38.44827350501902 40.0 38.0 41.0 34.0 41.0 38-39 38.33036040407124 40.0 38.0 41.0 34.0 41.0 40-41 38.20466558949002 40.0 38.0 41.0 33.5 41.0 42-43 38.251696385475476 40.0 38.0 41.0 34.0 41.0 44-45 38.14999335326375 40.0 38.0 41.0 33.0 41.0 46-47 37.98772890877365 40.0 38.0 41.0 33.0 41.0 48-49 37.798682310749385 40.0 37.5 41.0 33.0 41.0 50-51 37.61855680694984 40.0 37.0 41.0 33.0 41.0 52-53 37.41120778116847 40.0 37.0 41.0 32.0 41.0 54-55 37.22653419043382 40.0 36.0 41.0 32.0 41.0 56-57 36.979920146907546 39.0 36.0 41.0 31.5 41.0 58-59 36.72444183141277 39.0 35.0 41.0 31.0 41.0 60-61 36.4484435524352 39.0 35.0 41.0 31.0 41.0 62-63 36.1510425730795 38.0 35.0 40.0 30.5 41.0 64-65 35.78405257169983 37.5 35.0 40.0 30.0 41.0 66-67 35.43323154251668 37.0 35.0 40.0 29.0 41.0 68-69 35.07632256422278 36.5 34.0 39.0 29.0 41.0 70-71 34.69844743144531 36.0 34.0 39.0 29.0 41.0 72-73 34.28382129882678 35.5 34.0 38.5 28.5 40.0 74-75 33.851223188177286 35.0 34.0 37.5 28.0 39.5 76-77 33.11805190660686 35.0 33.0 37.0 27.0 39.0 78-79 33.16133788526433 35.0 33.0 36.5 27.0 39.0 80-81 32.992021819746384 35.0 33.0 36.0 27.0 38.5 82-83 32.70348280592076 35.0 33.0 36.0 27.0 37.0 84-85 32.40112759678759 35.0 33.0 35.0 26.5 37.0 86-87 31.99216418990124 35.0 33.0 35.0 26.0 36.0 88-89 31.497504852956162 35.0 32.0 35.0 24.5 36.0 90-91 31.277114595184408 35.0 32.0 35.0 24.0 36.0 92-93 30.99114076026082 34.0 32.0 35.0 23.0 35.0 94-95 30.83205584600372 34.0 32.0 35.0 23.0 35.0 96-97 30.576441031288304 34.0 31.0 35.0 20.0 35.0 98-99 30.113579512787943 34.0 31.0 35.0 10.0 35.0 100-101 26.695602754139113 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.028741135042452015 1101 2 0.09080318345949223 1101 3 0.13365674697142538 1101 4 0.05283388820578239 1101 5 0.1049334798461743 1101 6 0.08871141447821884 1101 7 0.16030686679486195 1101 8 0.08615007858261237 1101 9 0.1051988637576784 1101 10-11 0.1216001316761961 1101 12-13 0.19714638641275428 1101 14-15 0.22989892213306717 1101 16-17 0.3398301533113539 1101 18-19 0.32455050465259916 1101 20-21 0.28826605443692443 1101 22-23 0.2897521175964215 1101 24-25 0.17617292416375818 1101 26-27 0.19905162723679837 1101 28-29 0.24640185334902043 1101 30-31 0.35179067800984853 1101 32-33 0.4056676843828697 1101 34-35 0.3979084667522059 1101 36-37 0.3763679214077982 1101 38-39 0.35883451030600355 1101 40-41 0.3861372692752454 1101 42-43 0.34609623554137414 1101 44-45 0.394903339317608 1101 46-47 0.3355138350010378 1101 48-49 0.32599629575906874 1101 50-51 0.34967112197542605 1101 52-53 0.412920805214668 1101 54-55 0.398080065024935 1101 56-57 0.31305528183622755 1101 58-59 0.2856359334186749 1101 60-61 0.3604848496061024 1101 62-63 0.4451541196791311 1101 64-65 0.3943028904041128 1101 66-67 0.2050291531339994 1101 68-69 0.36385267271062816 1101 70-71 0.34938914813307065 1101 72-73 0.40026699625399687 1101 74-75 0.39231855357750334 1101 76-77 0.24570827944870643 1101 78-79 0.24887886941125004 1101 80-81 0.33532194346697963 1101 82-83 0.3334433149759235 1101 84-85 0.5452624760569407 1101 86-87 0.5071495397945895 1101 88-89 0.4633253134266262 1101 90-91 0.4036903319135554 1101 92-93 0.615929064492402 1101 94-95 0.46487605690568756 1101 96-97 0.3710916360704104 1101 98-99 0.42423202256848214 1101 100-101 0.8091769157947546 1102 1 0.07493215403077613 1102 2 0.0781395006786525 1102 3 0.12912147827309894 1102 4 0.07333107143767847 1102 5 0.0905556401593941 1102 6 0.14495784522312505 1102 7 0.13069205143136742 1102 8 0.12535393065759592 1102 9 0.10669377866938135 1102 10-11 0.13300071537656066 1102 12-13 0.20612844971996225 1102 14-15 0.23769355799704073 1102 16-17 0.2493747700859501 1102 18-19 0.3609785107484029 1102 20-21 0.28341632550598916 1102 22-23 0.1959946161595738 1102 24-25 0.12540731465958288 1102 26-27 0.25505778338738594 1102 28-29 0.17260646122591083 1102 30-31 0.21587698757039675 1102 32-33 0.259569986126607 1102 34-35 0.28993224182043775 1102 36-37 0.20310010462368666 1102 38-39 0.2313451763490093 1102 40-41 0.19493161662680336 1102 42-43 0.1502570096507938 1102 44-45 0.32625704775364994 1102 46-47 0.28676061095492855 1102 48-49 0.3388771629971785 1102 50-51 0.22076906027766796 1102 52-53 0.290495412648049 1102 54-55 0.12066841451908061 1102 56-57 0.28483387622756595 1102 58-59 0.17329624412504785 1102 60-61 0.32745417996012094 1102 62-63 0.31057382749973783 1102 64-65 0.20120361790345953 1102 66-67 0.29140649809744446 1102 68-69 0.16078954884827823 1102 70-71 0.16817077990619111 1102 72-73 0.05625881823041112 1102 74-75 0.13067700943716432 1102 76-77 0.09791197012133779 1102 78-79 0.04269743397287584 1102 80-81 0.12768914431596556 1102 82-83 0.08886115272404282 1102 84-85 0.10962566150822539 1102 86-87 0.11568507185764787 1102 88-89 0.19532346530120392 1102 90-91 0.2787893794568319 1102 92-93 0.3004921876689082 1102 94-95 0.20122201299967557 1102 96-97 0.0795668823004334 1102 98-99 0.17763656039766929 1102 100-101 0.3060842091231706 1103 1 0.08885396207023177 1103 2 0.0891364920678015 1103 3 0.02755492572615026 1103 4 0.018960605302510203 1103 5 0.14062068042051123 1103 6 0.09330869553475907 1103 7 0.13649779253122318 1103 8 0.1050916947090812 1103 9 0.09076889019546996 1103 10-11 0.13940698158675247 1103 12-13 0.17745088182670798 1103 14-15 0.23102593368235347 1103 16-17 0.2517648999270605 1103 18-19 0.14917669600540506 1103 20-21 0.25120144525189403 1103 22-23 0.2971820996999952 1103 24-25 0.1708716542415658 1103 26-27 0.145454967902225 1103 28-29 0.15664765605141895 1103 30-31 0.19190586065809612 1103 32-33 0.17113318690550017 1103 34-35 0.18180580036066374 1103 36-37 0.18200990216093516 1103 38-39 0.14055615418743628 1103 40-41 0.20861800286220955 1103 42-43 0.2376344107536852 1103 44-45 0.2043065581561052 1103 46-47 0.19619304489715716 1103 48-49 0.12785343726757503 1103 50-51 0.11738896179952718 1103 52-53 0.1205448113249048 1103 54-55 0.14004083585228244 1103 56-57 0.12782349372698576 1103 58-59 7.168364558651774E-5 1103 60-61 0.11810633825934502 1103 62-63 0.1858541390545625 1103 64-65 0.1672051182186891 1103 66-67 0.1432144577485417 1103 68-69 0.04932530503108978 1103 70-71 -0.06327678487470223 1103 72-73 -0.06828929161181208 1103 74-75 0.03279939685366173 1103 76-77 0.03703548277425739 1103 78-79 0.015570058806282816 1103 80-81 0.14588645172896264 1103 82-83 0.2462326483308317 1103 84-85 0.2836422767013147 1103 86-87 0.15038385165446755 1103 88-89 0.21336116471145417 1103 90-91 0.4178538371487406 1103 92-93 0.44212832930505286 1103 94-95 0.34139973651073063 1103 96-97 0.29994762719098134 1103 98-99 0.19128902993249497 1103 100-101 0.4309635055710963 1104 1 0.03479194999015789 1104 2 0.01761493738783315 1104 3 0.017446871439432243 1104 4 0.0482929468144917 1104 5 0.037959422279634225 1104 6 0.032414351628460736 1104 7 0.09404512681764032 1104 8 0.06336546064699178 1104 9 -0.03297632019850738 1104 10-11 0.09168623353649963 1104 12-13 0.1310948567057295 1104 14-15 0.1877887217851466 1104 16-17 0.19057320333141092 1104 18-19 0.17422696507568958 1104 20-21 0.0900634077409066 1104 22-23 0.1375811915635765 1104 24-25 0.15257332522809008 1104 26-27 0.15577417165565066 1104 28-29 0.18542500378165983 1104 30-31 0.1269047564819843 1104 32-33 0.08788702537034254 1104 34-35 0.18340298141842482 1104 36-37 0.21039303964829514 1104 38-39 0.189587384936992 1104 40-41 0.17374831501472698 1104 42-43 0.25965497554944506 1104 44-45 0.23439148285903855 1104 46-47 0.19472855868774275 1104 48-49 0.13422182141238181 1104 50-51 0.18900543688194915 1104 52-53 0.20137147117279142 1104 54-55 0.07507944327311833 1104 56-57 0.10765387677924565 1104 58-59 0.18945241519131173 1104 60-61 0.07624837792444339 1104 62-63 0.10188929447219408 1104 64-65 0.01848421907064335 1104 66-67 -0.015646458678887143 1104 68-69 0.12577400345929135 1104 70-71 0.11499825958291865 1104 72-73 -0.08873592116266593 1104 74-75 0.011719318902706277 1104 76-77 0.11851108823083223 1104 78-79 0.06145632479262275 1104 80-81 0.15792778278420627 1104 82-83 0.10535817959451066 1104 84-85 0.2173026011353798 1104 86-87 0.25518178742628805 1104 88-89 0.19070812009111293 1104 90-91 0.2788622792531079 1104 92-93 0.24029074779941695 1104 94-95 0.24770942497809756 1104 96-97 0.15839224985332478 1104 98-99 0.15656753414192082 1104 100-101 0.3804203284108816 1105 1 0.04445485812138372 1105 2 0.0943717823430319 1105 3 0.09665691494434014 1105 4 0.08771677718141291 1105 5 0.030673821018318392 1105 6 0.08623673094646023 1105 7 0.1230243647513376 1105 8 0.1444879744829919 1105 9 0.15996309737079883 1105 10-11 0.15037611546801344 1105 12-13 0.17505321494102333 1105 14-15 0.20596762503652855 1105 16-17 0.3008238925227289 1105 18-19 0.19035489568033626 1105 20-21 0.12446131161270557 1105 22-23 0.07376008343121043 1105 24-25 0.1554097767608127 1105 26-27 0.14406977733765558 1105 28-29 0.10743112444563252 1105 30-31 0.10869644520467148 1105 32-33 0.1640756313729952 1105 34-35 0.14916160413621782 1105 36-37 0.22412469046308559 1105 38-39 0.205827255769357 1105 40-41 0.23869546594620772 1105 42-43 0.3164048764159446 1105 44-45 0.27087735657148926 1105 46-47 0.31585737153316984 1105 48-49 0.263589624746686 1105 50-51 0.26289737092449883 1105 52-53 0.3449869887445658 1105 54-55 0.2717271180469183 1105 56-57 0.29765438904808406 1105 58-59 0.35197444749461226 1105 60-61 0.30787168198256154 1105 62-63 0.46056855156854226 1105 64-65 0.4936221047663878 1105 66-67 0.35189504932673543 1105 68-69 0.34982616381081044 1105 70-71 0.33656971065080654 1105 72-73 0.2612727115159075 1105 74-75 0.3023294310895892 1105 76-77 0.19403943661917822 1105 78-79 0.11831911453283084 1105 80-81 0.2231747058509086 1105 82-83 0.2697376607610451 1105 84-85 0.3151231818205602 1105 86-87 0.36910563392019213 1105 88-89 0.38990860124436466 1105 90-91 0.4536161530490368 1105 92-93 0.46423950573721484 1105 94-95 0.34100781019288107 1105 96-97 0.4717708146278241 1105 98-99 0.5190508616885161 1105 100-101 0.8011600066681019 1106 1 -0.07160915651053301 1106 2 -0.0719839561114739 1106 3 -0.028310620492334237 1106 4 -0.0026005524939449742 1106 5 -0.05435224208181921 1106 6 -0.04416656123461138 1106 7 0.026133646872963823 1106 8 0.02463978061010863 1106 9 0.04605180336073289 1106 10-11 0.15450688821606917 1106 12-13 0.05114649340467281 1106 14-15 0.06608102238450186 1106 16-17 0.07892082896534447 1106 18-19 0.1222149779848607 1106 20-21 0.14696428372061376 1106 22-23 0.029983220148182 1106 24-25 0.1252021864544588 1106 26-27 0.0581347064643154 1106 28-29 0.11427977556589752 1106 30-31 0.11037271406611637 1106 32-33 0.042802654692614794 1106 34-35 0.1966311971860577 1106 36-37 0.0938997247566391 1106 38-39 0.17695391329107935 1106 40-41 0.08127159763345304 1106 42-43 0.19387904193948913 1106 44-45 0.14786958336618738 1106 46-47 0.2372126527402969 1106 48-49 0.18307659319660985 1106 50-51 0.1610976452729176 1106 52-53 0.07021241169838532 1106 54-55 0.18354970606703347 1106 56-57 0.1718272285542568 1106 58-59 0.2898793400414803 1106 60-61 0.2942575987635436 1106 62-63 0.2423079205671712 1106 64-65 0.2528484422149546 1106 66-67 0.08486898622660277 1106 68-69 0.18344481950354208 1106 70-71 0.16726289766498326 1106 72-73 0.05597961595120893 1106 74-75 0.11640350516366027 1106 76-77 0.17098319519256222 1106 78-79 -0.0021591662170550308 1106 80-81 0.03454004650020437 1106 82-83 0.040354030216917636 1106 84-85 0.13005870994127378 1106 86-87 0.10581802532393425 1106 88-89 0.02267769132209807 1106 90-91 0.15479127879206445 1106 92-93 0.312287059463781 1106 94-95 0.1065743396853378 1106 96-97 0.3178878699547525 1106 98-99 0.306514053275162 1106 100-101 0.6049579033301988 1107 1 -0.042520046557378066 1107 2 -0.006973166069890624 1107 3 -0.0600697657105016 1107 4 0.020534770417789616 1107 5 0.030699813289515987 1107 6 0.06312245397671745 1107 7 -0.008326061532741846 1107 8 0.04609964691886148 1107 9 0.05403896057953972 1107 10-11 0.057695582357872865 1107 12-13 0.040302133175188715 1107 14-15 0.10712865647050052 1107 16-17 0.07941317066753584 1107 18-19 0.10996664912545384 1107 20-21 0.0566343716199782 1107 22-23 0.053299184146283096 1107 24-25 0.13715692091416543 1107 26-27 0.1780820721151386 1107 28-29 0.09887338575590121 1107 30-31 -0.016901458258736568 1107 32-33 0.033615223775193215 1107 34-35 0.0670384817799885 1107 36-37 0.021704030246404216 1107 38-39 0.07692454332423182 1107 40-41 0.07973583259318673 1107 42-43 -0.13003653256442504 1107 44-45 -0.06507393997715383 1107 46-47 0.03588584606626455 1107 48-49 -0.11062760176101705 1107 50-51 -0.1316402883025347 1107 52-53 -0.049985282917120344 1107 54-55 -0.002502823013436739 1107 56-57 -0.13774864386008545 1107 58-59 -0.23210318535460317 1107 60-61 -0.10985324917582062 1107 62-63 -0.10372755498417519 1107 64-65 -0.030527314403819616 1107 66-67 -0.3110612206263923 1107 68-69 -0.12766355462141377 1107 70-71 0.02485003383003459 1107 72-73 -0.056476197512658644 1107 74-75 -0.14888729961553793 1107 76-77 -0.17251656795286863 1107 78-79 -0.06949313116258082 1107 80-81 0.1310497575376246 1107 82-83 0.16549609032534818 1107 84-85 -0.016766280847711812 1107 86-87 -0.07393511957842591 1107 88-89 0.2032670907641716 1107 90-91 -0.10041636371543561 1107 92-93 0.09904636258499977 1107 94-95 0.09821400493087395 1107 96-97 -0.08980021889203016 1107 98-99 -0.15767427242618837 1107 100-101 -0.13987403519288222 1108 1 -0.24897001844038158 1108 2 -0.14169110880025926 1108 3 -0.05711844241078978 1108 4 0.01126759502136565 1108 5 -0.07079231992130275 1108 6 -0.05216390330115672 1108 7 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64-65 -0.6105714362288381 2316 66-67 -0.7685318217218722 2316 68-69 -0.7711791933071694 2316 70-71 -0.7446323601870404 2316 72-73 -0.7302453722207147 2316 74-75 -0.7962317898103208 2316 76-77 -0.703384312177235 2316 78-79 -0.7384074465970158 2316 80-81 -0.7544098388584644 2316 82-83 -0.8952393023885321 2316 84-85 -0.7250799150905678 2316 86-87 -0.7712424252347105 2316 88-89 -0.6286701996412525 2316 90-91 -0.6382854763122019 2316 92-93 -0.60201508414724 2316 94-95 -0.5928205724229727 2316 96-97 -0.5191328382739471 2316 98-99 -0.6119858557396505 2316 100-101 -0.5952068162534552 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 10.0 4 19.0 5 30.0 6 66.0 7 143.0 8 351.0 9 635.0 10 971.0 11 1687.0 12 2533.0 13 3596.0 14 4604.0 15 6005.0 16 7428.0 17 10069.0 18 9611.0 19 7015.0 20 6754.0 21 7180.0 22 7835.0 23 9038.0 24 10897.0 25 13140.0 26 16500.0 27 20982.0 28 26385.0 29 33135.0 30 40926.0 31 51385.0 32 65013.0 33 84616.0 34 116014.0 35 172851.0 36 296401.0 37 561030.0 38 657172.0 39 132522.0 40 79.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.385067665138514 18.114459254998035 13.155152533563438 24.345320546300012 2 15.83840432724814 19.08694426688276 40.15874853406752 24.91590287180158 3 20.15437202181136 23.001104154985956 28.32696406862451 28.517559754578176 4 12.946122856008211 15.120255603701876 36.0677489034982 35.86587263679172 5 14.220073646676276 37.01582090966771 34.05561200505404 14.70849343860198 6 34.74095970484298 34.81883345375738 16.506047691276084 13.934159150123563 7 30.471742145214204 30.933491121679722 19.84166090406516 18.753105829040912 8 26.835620970809295 35.990881600450216 18.628390412093452 18.545107016647034 9 26.302204661605643 16.035114896279463 19.227099896881235 38.43558054523366 10-11 25.898862881527652 25.950275599004286 26.80812572548125 21.342735793986808 12-13 26.126826936282498 22.706795925149738 27.58514694643084 23.581230192136925 14-15 22.85353402982183 24.54639694476583 25.090255129833615 27.509813895578727 16-17 23.238664801099212 27.674971908504443 25.74904539237618 23.337317898020167 18-19 23.045807125552862 26.898125452638546 27.33557295495442 22.720494466854174 20-21 23.77338361648709 25.98794193981537 27.47540602752042 22.763268416177105 22-23 23.1695935738391 25.93373598182525 27.331228881659715 23.565441562675932 24-25 23.130262734669895 25.993039424668485 27.660675517168066 23.216022323493558 26-27 23.134390323785976 26.314587667624632 27.342278625613265 23.208743382976127 28-29 22.977363242811187 26.504200123040896 27.394736919356212 23.123699714791705 30-31 23.204288174412152 26.26437633578231 27.38837824050068 23.142957249304864 32-33 22.96498333992775 26.18966293416467 27.42729526017747 23.418058465730113 34-35 23.22519580958883 26.368738066591824 27.322131673115724 23.083934450703623 36-37 23.278138141462733 26.202808152885904 27.343740826661435 23.17531287898993 38-39 23.159811400233956 26.42518650195324 27.34839187604432 23.06661022176848 40-41 23.304251009599053 26.2669576995819 27.401252196375925 23.027539094443117 42-43 23.261962564734624 26.162623194465358 27.2965930998099 23.278821140990118 44-45 23.190832376467903 26.369016515679032 27.21358829987729 23.22656280797577 46-47 23.306941174200833 26.461438899733004 27.002479559987147 23.229140366079015 48-49 23.302054221275096 26.48795624517282 26.965793316278607 23.244196217273476 50-51 23.312751575399858 26.653509327423997 26.861311970690394 23.172427126485758 52-53 23.437617296392336 26.533151656293207 26.802165362173515 23.22706568514094 54-55 23.41548819572071 26.462343620426292 26.87723109213303 23.24493709171997 56-57 23.202458487546213 26.75251215582946 26.794218041895018 23.250811314729308 58-59 23.266416910401635 26.511039731543118 26.969399161239988 23.253144196815263 60-61 23.282468597884126 26.570829187796235 26.95422802847866 23.19247418584098 62-63 23.241078591260948 26.52735498897313 27.007660311100807 23.223906108665123 64-65 23.21745559338609 26.59192631418709 26.866499399692444 23.324118692734373 66-67 23.133388883553792 26.71484746683866 26.829938745188453 23.321824904419096 68-69 23.189833722562295 26.557485462099134 26.98996363794955 23.26271717738902 70-71 23.211285466537813 26.369287910947826 26.937197030391996 23.482229592122366 72-73 23.255100555943926 26.564973561526013 26.733786436511387 23.44613944601867 74-75 23.14494204339543 26.73071995685702 26.648631715876974 23.475706283870572 76-77 23.20278485858153 26.676105559569546 26.699951404898464 23.42115817695046 78-79 23.15306588303204 26.638553774302732 26.770414294335264 23.437966048329965 80-81 23.176354155090674 26.66095642674573 26.6759274596841 23.486761958479498 82-83 23.312976567843467 26.74642470589765 26.686016986290113 23.254581739968774 84-85 23.250447662301173 26.69465866535828 26.557151903246236 23.49774176909432 86-87 23.140461861153618 26.549448039413072 26.76270386894109 23.547386230492222 88-89 23.34165678349236 26.57111888641391 26.575358298903044 23.511866031190685 90-91 23.12102462759113 26.796738980007934 26.65683195129923 23.42540444110171 92-93 23.207803281928992 26.711247236057268 26.67865295183301 23.40229653018073 94-95 23.08371514594779 26.657757996887067 26.761697137238876 23.496829719926268 96-97 23.160639361896195 26.574721175560324 26.696273743848458 23.568365718695024 98-99 23.028994484090756 26.505132819219675 26.659626924414052 23.80624577227552 100-101 23.05199080438176 26.69639614629454 26.599378348026136 23.65223470129757 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 47.0 1 35.0 2 54.5 3 105.5 4 92.5 5 74.5 6 185.0 7 387.5 8 607.0 9 681.5 10 588.0 11 527.5 12 585.0 13 706.5 14 776.5 15 847.5 16 992.5 17 1152.0 18 1299.5 19 1498.0 20 1746.0 21 2091.0 22 2613.0 23 3419.5 24 4554.5 25 5846.0 26 7841.0 27 10491.0 28 13124.5 29 16269.5 30 20013.5 31 24234.0 32 29144.5 33 33885.5 34 38653.0 35 43656.0 36 50024.5 37 56256.5 38 60842.5 39 64499.0 40 67914.5 41 71769.0 42 74567.0 43 78192.5 44 82566.0 45 86291.0 46 90430.0 47 93768.0 48 97559.5 49 98573.5 50 95206.0 51 92941.5 52 92038.0 53 91314.5 54 89763.0 55 84835.0 56 78160.0 57 70857.5 58 65067.5 59 59655.5 60 50947.0 61 41313.5 62 32623.0 63 24932.5 64 18463.0 65 13775.5 66 9943.5 67 7564.5 68 6000.5 69 4648.0 70 3548.0 71 2430.5 72 1733.0 73 1446.0 74 1168.5 75 865.0 76 580.5 77 332.0 78 201.5 79 104.0 80 51.0 81 28.5 82 14.5 83 7.5 84 4.0 85 4.0 86 3.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2765629370438756 2 0.020170852572874022 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.096762221712476E-4 12-13 6.290286665137428E-5 14-15 0.0 16-17 4.4032006655962E-4 18-19 0.0 20-21 0.0 22-23 6.290286665137428E-5 24-25 0.0023483736883179733 26-27 0.0025161146660549714 28-29 0.0023483736883179733 30-31 0.0010274134886391134 32-33 0.0014887011774158579 34-35 0.00176128026623848 36-37 4.193524443424952E-5 38-39 1.048381110856238E-4 40-41 7.967696442507409E-4 42-43 0.0036064310213454586 44-45 0.004487071154464699 46-47 0.014048306885473589 48-49 0.014404756463164711 50-51 0.006499962887308676 52-53 0.007464473509296414 54-55 2.725790888226219E-4 56-57 0.0030822404659173394 58-59 0.0015725716662843572 60-61 0.0015306364218501075 62-63 0.0 64-65 7.548343998164913E-4 66-67 0.0 68-69 0.0 70-71 1.8870859995412284E-4 72-73 0.0015096687996329827 74-75 0.0 76-77 0.19959079588481057 78-79 0.1076268048405014 80-81 0.0010064458664219885 82-83 0.0 84-85 7.967696442507409E-4 86-87 0.1250718665251492 88-89 0.5878272888570927 90-91 0.30763695316965445 92-93 0.2898354419073156 94-95 0.0025370822882720963 96-97 0.002683855643791969 98-99 0.0030193375992659654 100-101 6.290286665137429E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2384629.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.56633018417625 #Duplication Level Percentage of deduplicated Percentage of total 1 72.75020661806563 40.42462001906479 2 14.692404959647975 16.328060503748564 3 5.469776603358331 9.118062383276733 4 2.4804018433787665 5.513073112744958 5 1.3824836476748499 3.8409771420462544 6 0.8027547534782834 2.6763681413214777 7 0.4963191865330766 1.9305045056947086 8 0.3762403039648997 1.6725034366966758 9 0.2690983391549216 1.3457526448946233 >10 1.1880530037390404 11.522505174534293 >50 0.06379192126233868 2.4420447917970347 >100 0.027413652372046172 2.579484953939295 >500 7.536909785175352E-4 0.24734234548851988 >1k 3.014763914065718E-4 0.3587008447521601 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3413 0.14312498925409362 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2500 0.1048381110856238 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.387048886849904E-5 2 0.0 0.0 0.0 0.0 8.387048886849904E-5 3 0.0 0.0 0.0 0.0 8.387048886849904E-5 4 0.0 0.0 0.0 0.0 8.387048886849904E-5 5 4.193524443424952E-5 0.0 0.0 0.0 1.2580573330274857E-4 6 8.387048886849904E-5 0.0 0.0 0.0 1.6774097773699807E-4 7 1.2580573330274857E-4 0.0 0.0 0.0 1.6774097773699807E-4 8 1.2580573330274857E-4 0.0 0.0 8.387048886849904E-5 1.6774097773699807E-4 9 1.2580573330274857E-4 0.0 0.0 1.2580573330274857E-4 1.6774097773699807E-4 10-11 1.2580573330274857E-4 0.0 0.0 1.2580573330274857E-4 1.6774097773699807E-4 12-13 2.096762221712476E-4 0.0 0.0 2.096762221712476E-4 3.774171999082457E-4 14-15 2.5161146660549713E-4 0.0 0.0 2.096762221712476E-4 4.4032006655961997E-4 16-17 2.5161146660549713E-4 0.0 0.0 2.096762221712476E-4 4.6128768877674474E-4 18-19 2.935467110397466E-4 0.0 0.0 2.096762221712476E-4 5.451581776452438E-4 20-21 3.3548195547399614E-4 0.0 0.0 2.5161146660549713E-4 6.290286665137429E-4 22-23 3.774171999082457E-4 0.0 0.0 3.1451433325687143E-4 6.499962887308676E-4 24-25 4.193524443424952E-4 0.0 0.0 4.193524443424952E-4 7.128991553822418E-4 26-27 4.193524443424952E-4 0.0 0.0 5.24190555428119E-4 7.128991553822418E-4 28-29 5.24190555428119E-4 0.0 0.0 9.435429997706143E-4 7.548343998164913E-4 30-31 6.290286665137429E-4 0.0 0.0 0.002432244177186472 7.548343998164913E-4 32-33 6.290286665137429E-4 0.0 0.0 0.005220937932064065 7.548343998164913E-4 34-35 7.548343998164913E-4 0.0 0.0 0.008932207064495146 7.967696442507409E-4 36-37 7.967696442507409E-4 0.0 0.0 0.014866044151941454 7.967696442507409E-4 38-39 7.967696442507409E-4 0.0 0.0 0.025999851549234704 7.967696442507409E-4 40-41 8.177372664678656E-4 0.0 0.0 0.03790946096856157 7.967696442507409E-4 42-43 9.225753775534895E-4 0.0 0.0 0.05074164576544192 8.387048886849904E-4 44-45 0.0010064458664219885 0.0 0.0 0.06483188789534976 8.387048886849904E-4 46-47 0.0010903163552904876 0.0 0.0 0.08322049257976817 8.596725109021152E-4 48-49 0.001132251599724737 0.0 0.0 0.10553004261878891 9.225753775534895E-4 50-51 0.001132251599724737 0.0 0.0 0.13008312823504203 9.225753775534895E-4 52-53 0.001132251599724737 0.0 0.0 0.15415395854030123 9.225753775534895E-4 54-55 0.001132251599724737 0.0 0.0 0.1793570404452852 9.225753775534895E-4 56-57 0.001132251599724737 0.0 0.0 0.20986493077120172 9.64510621987739E-4 58-59 0.001132251599724737 0.0 0.0 0.24559375902918232 0.0010064458664219885 60-61 0.001132251599724737 0.0 0.0 0.28501288879737685 0.0010274134886391131 62-63 0.001132251599724737 0.0 0.0 0.3262771693206784 0.0011741868441589867 64-65 0.0011741868441589867 0.0 0.0 0.3706446579321144 0.001237089710810361 66-67 0.0011741868441589867 0.0 0.0 0.41928954147584385 0.0012580573330274857 68-69 0.0011741868441589867 0.0 0.0 0.4765311501285944 0.0012580573330274857 70-71 0.0011951544663761113 0.0 0.0 0.5440468936677362 0.0013419278218959846 72-73 0.001216122088593236 0.0 0.0 0.6140158490062815 0.0014257983107644836 74-75 0.001216122088593236 0.0 0.0 0.6859347932110194 0.0015096687996329827 76-77 0.001216122088593236 0.0 0.0 0.7650665994584482 0.0015096687996329827 78-79 0.0012580573330274857 0.0 0.0 0.8572612343471457 0.0015096687996329827 80-81 0.0016983773995871056 0.0 0.0 0.96501384492095 0.0015096687996329827 82-83 0.0018032155106727293 0.0 0.0 1.0791196450265428 0.0017193450218042302 84-85 0.0018032155106727293 0.0 0.0 1.1939593119097354 0.0017193450218042302 86-87 0.0018032155106727293 0.0 0.0 1.3177311858574225 0.0017822478884556045 88-89 0.0018032155106727293 0.0 0.0 1.455425560957281 0.001845150755106979 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5385 0.0 36.488476 1 GGTATCA 3320 0.0 34.249264 1 ATCAACG 6305 0.0 30.395615 3 TCAACGC 6300 0.0 30.344442 4 CAACGCA 6435 0.0 29.634129 5 TATCAAC 6765 0.0 28.615885 2 AACGCAG 6700 0.0 28.462032 6 GTACATG 7995 0.0 27.314018 1 TACATGG 7715 0.0 27.244738 2 ACATGGG 7735 0.0 26.248112 3 ACGCAGA 8030 0.0 23.570675 7 CGCAGAG 8080 0.0 23.24869 8 CATGGGG 4775 0.0 22.054348 4 GTACCTG 2225 0.0 20.954988 1 GTATAGC 1030 0.0 20.785797 1 GCAGAGT 9790 0.0 18.89716 9 TACCTGG 2315 0.0 18.856092 2 GGACCGT 310 5.682523E-9 18.362602 6 GAGTACT 4595 0.0 17.704891 12-13 GTATAGG 680 0.0 17.491316 1 >>END_MODULE