FastQCFastQC Report
Thu 26 May 2016
SRR936295_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936295_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741669
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37450.21502363537503397No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25730.14773185949798728No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24670.14164574324972196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18940.10874626579447644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG17050.030.261062
GTACCTG17450.030.1202961
GTACATG64450.026.8164711
TACATGG63200.026.5884672
ACATGGG65000.024.395013
GTATCAA72100.023.314431
TACCGGG1852.6300404E-723.0278192
CATGGGG43800.021.9370924
CCGTATC1303.014168E-421.8450229
CAACGCA83300.020.1716045
TCAACGC83200.020.138964
ATCAACG83300.020.115363
TATCAAC84100.019.8683012
AACGCAG85800.019.8039476
GAGTACA51550.019.5651261
AGTACAT46950.019.3574282
GGTATCA55850.019.245731
ACCTGGG27200.018.7942243
GTACTTT39750.018.10145214-15
AGAGTAC67750.017.99000410-11
Produced by FastQC (version 0.11.5)